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Vitamin C content in fruit juices

Objective Problem statement Hypothesis Variables


To determine the vitamin C content in various fruit juices Do different types of fruit juices contain similar amounts of vitamin C?

Apparatus and materials

_____________contains higher concentration of vitamin C compared to _______________. Manipulated : Responding : Constant : Materials : freshly prepared lime and orange juices, 0.1% ascorbic acid solution, DCPIP solution Apparatus : 5ml syringe(with needle), 1ml syringe, specimen tubes, 5ml measuring cylinder Measure and record the volume of fruit juice needed to decolourise 1 ml DCPIP using a ___________. Calculate the % of vitamin C in fruit juice by using formula :

Technique

Vol of 0.1% ascorbic acid used X 0.1% Volume of fruit juice used Calculate the concentration of vitamin C in fruit juice by using formula :

Vol of 0.1% ascorbic acid mg cm Volume of fruit juice

Procedure

1. 1 ml of DCPIP solution is placed in a specimen tube using a 1 ml syringe. 2. 5 ml syringe was filled with 5 ml of 0.1% ascorbic acid solution. 3. The needle of the 5 ml syringe was immersed into the DCPIP solution. 4. Ascorbic acid solution was added drop by drop until the DCPIP solution, and gently stirred with the needle of the syringe. (Do not shake the tube vigorously). 5. Ascorbic acid solution is added continuously until the DCPIP solution is decolourise.

6. The volume of ascorbic acid solution used measured using a syringe and recorded. 7. Steps 1-6 are repeated using fresh orange and lime juice. 8. The volume of fruit juices required to decolourise the DCPIP solution in each fruit juice is measured using a syringe and recorded in a table. 9. The percentage and the concentration of vitamin C in each fruit juice are calculated using the above formula. Precaution: 1. The juice must be freshly prepared. 2. Do not shake the DCPIP too vigorously. Recording data/ result
Juice Volume of juice used (cm) Percentage of Vitamin C in juice (%) (show your calculation) Concentration of vitamin C in juice (mg cm) (show your calculation)

0.1% ascorbic acid Lime juice Orange juice

Discussion

Conclusion

_____________contains higher concentration of vitamin C compared to ____________. Hypothesis is accepted.