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PRINCIPLES

OF RIBOSOME
STRUCTURE

A. S. SPIRIN

. .

.., 1998

Several basic principles


of structural organization
of the ribosome are formulated: 1) the ribosome
is constructed of two
unequal subparticles (ribosomal subunits); 2) the
core of each ribosomal
subparticle is formed by
high-polymer ribosomal
RNA self-folded into a
compact body of specific
conformation; 3) diverse
ribosomal proteins are
assembled on the RNA
core, each protein recognizing its specific binding
site.
: 1)
; 2) fl


,
fl
fl ; 3)
fl
,

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65

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66

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, 11, 1998

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2:

. 2. Escherichia coli
() :
,
(30S) (50S) ;
,
(50S) , (30S) ( .. , , )

,
2 : 1.
( ) ,
(S). 3 106
70S 70S-,
(, )
80S-. :
70S

50S + 30S;

80S

60S + 40S.

..


,

. , . 23S ( 3000 ) 16S
( 1500 ), 28S
(47004800 ) 18S (
1900 ). ,

( , ), , 23S
,
16S (. 3).
.
, , -, ( )
( )
, -,

.

, ,
,
, . ,
, ,
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, ,
. ,

67

50S

23S

10
30S

16S

. 3.
: (50S)
23S . (30S) 16S
(.. , , )


.

()
.
, 5S
5S 5,8S
.

.
3:

,
(. 4). ,

.

68

, . ,
. : .
. , . 23S
- 32 , 16S
21 .
, , .
.
. -,
,
, ,

S1
L1
L2
L3
L4
S3
L9 L10
S4
L5
L11
S5/L6
L7/L12 S6
S7
L13
S8
L15 L16
L14
S10
L17
S9/S11
L18
S12/L20
L19
L21
L22
L23
S13
L24
L25
S15 S14
S16
S18
S17
S19
S2

L29
L30

S20/L26
L27
S21

L32/L33

. 4. (Escherichia coli) 70S



. S (small)
(30S) , L (large)
(50S) ( .. , , )

, 11, 1998

;
. -, , , , ,
. -,

.
.
,

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.


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-

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-, (. 5). - .
(. 3, ). . ,

(. 5).

, (. 3,
). (, )
- , .
. 2 ( ) . 5 ,
,

3'

30S

50S

5'

. 5.
( )
() . .
, . (- -)
-
, .
5'- ( )
3'- ( ), ,
.
,

69

.
, , ,
, - ,
: , .
, ,
. 3 ( ) . 2 (),

(. 2, ).

: -

1 (EF1).
.
, . 2 (EF2),
.
. 3 ( )
( ), -, .

70


(. 3, ).

.

1. .. : . .: . ., 1986. 300 .
2. . . .: ,
1978. 700 .

* * *
, , . ,
,
, ,
.

, , , . (
),
, ( ) ,
, .
300 .

, 11, 1998