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Ch. 9 - 2
1. Introduction
Classic
Classic
methods for organic structure determination Require large quantities of sample and are time consuming
Ch. 9 - 4
Spectroscopic methods for organic structure determination a) Mass Spectroscopy (MS) Molecular Mass & characteristic fragmentation pattern b) Infrared Spectroscopy (IR) Characteristic functional groups c) Ultraviolet Spectroscopy (UV) Characteristic chromophore d) Nuclear Magnetic Resonance (NMR)
Ch. 9 - 5
Spectroscopic methods for organic structure determination Combination of these spectroscopic techniques provides a rapid, accurate and powerful tool for Identification and Structure Elucidation of organic compounds Rapid Effective in mg and microgram quantities
Ch. 9 - 6
General steps for structure elucidation 1. Elemental analysis Empirical formula e.g. C2H4O 2. Mass spectroscopy Molecular weight Molecular formula e.g. C4H8O2, C6H12O3 etc. Characteristic fragmentation pattern for certain functional groups
Ch. 9 - 7
General steps for structure elucidation 3. From molecular formula Double bond equivalent (DBE) 4. Infrared spectroscopy (IR) Identify some specific functional groups e.g. C=O, CO, OH, COOH, NH2 etc.
Ch. 9 - 8
General steps for structure elucidation 5. UV Sometimes useful especially for conjugated systems e.g. dienes, aromatics, enones 6. 1H, 13C NMR and other advanced NMR techniques Full structure determination
Ch. 9 - 9
Electromagnetic spectrum
X-rays
ultraviolet
visible
infrared
microwave
radiowave
0.1nm
200nm
400nm
800nm IR
50m
X-Ray Crystallography
NMR
Ch. 9 - 10
graph that shows the characteristic energy absorption frequencies and intensities for a sample in a magnetic field is called a nuclear magnetic resonance (NMR) spectrum
Ch. 9 - 11
Ch. 9 - 12
1.
The number of signals in the spectrum tells us how many different sets of protons there are in the molecule The position of the signals in the spectrum along the x-axis tells us about the magnetic environment of each set of protons arising largely from the electron density in their environment
Ch. 9 - 13
2.
3.
The area under the signal tells us about how many protons there are in the set being measured The multiplicity (or splitting pattern) of each signal tells us about the number of protons on atoms adjacent to the one whose signal is being measured
4.
Ch. 9 - 14
Typical 1H NMR spectrum Chemical Shift () Integration (areas of peaks no. of H) Multiplicity (spin-spin splitting) and coupling constant
Ch. 9 - 15
Record as:
4.35 (2H, t, J = 7.2 Hz, Hc) 2.05 (2H, sextet, J = 7.2 Hz, Hb) 1.02 (3H, t, J = 7.2 Hz, Ha) chemical shift () in ppm coupling constant no. of H (integration) multiplicity in Hz
Ch. 9 - 16
The position of a signal along the x-axis of an NMR spectrum is called its chemical shift The chemical shift of each signal gives information about the structural environment of the nuclei producing that signal Counting the number of signals in a 1H NMR spectrum indicates, at a first approximation, the number of distinct proton environments in a molecule
Ch. 9 - 17
Ch. 9 - 18
Ch. 9 - 19
"upfield" (more shielded) "downfield" (deshielded) 15 (low field strength) -10 (high field strength)
ppm
Ch. 9 - 20
Me Si Me Me
Resonance position at higher field than other organic compounds Unreactive and stable, not toxic Volatile and easily removed (B.P. = 28oC) Ch. 9 - 21
NMR solvent Normal NMR solvents should not contain hydrogen Common solvents
Ch. 9 - 23
area under each signal in a 1H NMR spectrum is proportional to the number of hydrogen atoms producing that signal It is signal area (integration), not signal height, that gives information about the number of hydrogen atoms
Ch. 9 - 24
Ha R O
Ha Hb
Hb Hb
2H
3H
a
Ch. 9 - 25
is caused by the magnetic effect of nonequivalent hydrogen atoms that are within 2 or 3 bonds of the hydrogens producing the signal The n+1 rule Rule of Multiplicity: If a proton (or a set of magnetically equivalent nuclei) has n neighbors of magnetically equivalent protons. Its multiplicity is n + 1
Ch. 9 - 26
(1) Hb
Examples
Hb Ha C C Cl Hb Ha H : multiplicity = 3 + 1 = 4 (a quartet) Hb: multiplicity = 2 + 1 = 3 (a triplet)
a
(2) Ha Hb Cl C C Cl Cl Hb
Ch. 9 - 27
Ch. 9 - 28
(3) Hb
Examples
Hb Ha C C H Br
b
Hb Hb
Ch. 9 - 29
Pascals Triangle Use to predict relative intensity of various peaks in multiplet Given by the coefficient of binomial expansion (a + b)n singlet (s) doublet (d) triplet (t) quartet (q) quintet sextet 1 11 121 1331 14641 1 5 10 10 5 1
Ch. 9 - 30
Pascals Triangle
Ha Hb
For
Br
Br
Cl Cl
Ha Hb
For
Cl
Br
Cl Br
Count the number of signals to determine how many distinct proton environments are in the molecule (neglecting, for the time being, the possibility of overlapping signals) Use chemical shift tables or charts to correlate chemical shifts with possible structural environments Ch. 9 - 32
2.
3.
Determine the relative area of each signal, as compared with the area of other signals, as an indication of the relative number of protons producing the signal Interpret the splitting pattern for each signal to determine how many hydrogen atoms are present on carbon atoms adjacent to those producing the signal and sketch possible molecular fragments Join the fragments to make a molecule in a fashion that is consistent with the data
Ch. 9 - 33
4.
5.
Example: 1H NMR (300 MHz) of an unknown compound with molecular formula C3H7Br
Ch. 9 - 34
(ppm): Integral:
3.4 2
1.8 2
1.1 3
Ch. 9 - 36
(ppm):
3.4
1.8 sextet
5 H's on adjacent C
1.1 triplet
2 H's on adjacent C
Multiplicity: triplet
2 H's on adjacent C
Ch. 9 - 37
Complete structure:
most downfield signal Br 2 H's from integration triplet
CH2 CH2
Ch. 9 - 38
Ch. 9 - 39
Ch. 9 - 40
Ch. 9 - 41
1H:
Spin quantum number (I) I = (two spin states: + or -) (similar for 13C, 19F, 31P) I=0 These nuclei do not give an NMR spectrum
Ch. 9 - 42
Ch. 9 - 43
Ch. 9 - 44
All protons do not absorb energy at the same frequency in a given external magnetic field Lower chemical shift values correspond with lower frequency Higher chemical shift values correspond with higher frequency
"upfield" (more shielded) "downfield" (deshielded) 15 (low field strength) -10 (high field strength)
ppm
Ch. 9 - 45
Ch. 9 - 46
Deshielding
by electronegative groups
Electro4.0 negativity
Ch. 9 - 47
Shielding
and deshielding by circulation of electrons If we were to consider only the relative electronegativities of carbon in its three hybridization states, we might expect the following order of protons attached to each type of carbon:
In fact, protons of terminal alkynes absorb between 2.0 and 3.0, and the order is (higher (lower 2 < sp < sp3 sp frequency) frequency)
Ch. 9 - 49
This upfield shift (lower frequency) of the absorption of protons of terminal alkynes is a result of shielding produced by the circulating electrons of the triple bond
H
Shielded ( 2 3 ppm)
Ch. 9 - 50
Aromatic system
Shielded region
Deshielded region
Ch. 9 - 51
e.g.
Hd Hc
H Ha
(ppm) H & H : 7.9 & 7.4 (deshielded) H & H : 0.91 1.2 (shielded)
c d
Ch. 9 - 52
Alkenes
H
Deshielded ( 4.5 7 ppm)
Ch. 9 - 53
Aldehydes
R H
Ch. 9 - 54
Me Me Si Me Me
as a reference standard (0 ppm) Reasons for the choice of TMS as reference Resonance position at higher field than other organic compounds Unreactive and stable, not toxic Volatile and easily removed (B.P. = 28oC)
Ch. 9 - 55
chemical shift of a proton, when expressed in hertz (Hz), is proportional to the strength of the external magnetic field Since spectrometers with different magnetic field strengths are commonly used, it is desirable to express chemical shifts in a form that is independent of the strength of the external field
Ch. 9 - 56
Since chemical shifts are always very small (typically 5000 Hz) compared with the total field strength (commonly the equivalent of 60, 300, or 600 million hertz), it is convenient to express these fractions in units of parts per million (ppm) This is the origin of the delta scale for the expression of chemical shifts relative to TMS
(observed shift from TMS in hertz) x 106 (operating frequency of the instrument in hertz)
Ch. 9 - 57
For example, the chemical shift for benzene protons is 2181 Hz when the instrument is operating at 300 MHz. Therefore
= 2181 Hz x 106 300 x 106 Hz = 7.27 ppm
Thus, the chemical shift expressed in ppm is the same whether measured with an instrument operating at 300 or 60 MHz (or any other field strength) Ch. 9 - 58
or more protons that are in identical environments have the same chemical shift and, therefore, give only one 1H NMR signal equivalent protons are chemical shift equivalent in 1H NMR spectra
Ch. 9 - 59
Chemically
replacing the hydrogens by a different atom gives the same compound, the hydrogens are said to be homotopic hydrogens have identical environments and will have the same chemical shift. They are said to be chemical shift equivalent
Ch. 9 - 60
Homotopic
Br H H C H C H H
H
H C H
Br C H H
same compounds
same compounds
H Br C H
H H C
H C H
H C H
H C H H
H H C H
H H C H
H C H
H C Br H
C H
Br
Ethane
The six hydrogens of ethane are homotopic and are, therefore, chemical shift equivalent Ethane, consequently, gives only one Ch. 9 - 61 signal in its 1H NMR spectrum
Br H
If
replacing hydrogens by a different atom gives different compounds, the hydrogens are said to be heterotopic atoms have different chemical shifts and are not chemical shift equivalent
Heterotopic
Ch. 9 - 62
same Cl Br compounds H C C H these 3 H H Hs of the H Br CH3 group H Cl C C H are homotopic H H the CH3 H Br group gives H C C H H Br only one 1H Cl H NMR signal H C C
Cl H
C H
Ch. 9 - 63
H H C H
CH3CH2Br
Br C H H
two sets of hydrogens that are heterotopic with respect to each other two 1H NMR signals
Ch. 9 - 64
Other
H (1) H C
examples
CH3 C CH3
2 1H NMR signals
H (2) H H
CH3 H CH3
Ch. 9 - 65
4 1H NMR signals
Other
H (3) H3C
examples
H H CH3 H H H
3 1H NMR signals
Ch. 9 - 66
Application
(1)
13C
NMR signal
13C
NMR signals
Ch. 9 - 67
(3) HO OH
13C
NMR signals
(4)
HO OH
13C
NMR signals
Ch. 9 - 68
replacement of each of two hydrogen atoms by the same group yields compounds that are enantiomers, the two hydrogen atoms are said to be enantiotopic
Ch. 9 - 69
Enantiotopic
hydrogen atoms have the same chemical shift and give only one 1H NMR signal:
H G Br H3C
enantiotopic
H H3C H Br
enantiomer
G H3C H Br
Ch. 9 - 70
H H3C H
b
OH Ha CH3
Hb
H H3C G Ha
OH CH3
diastereotopic
Ch. 9 - 71
diastereomers
chirality centre
H H3C
OH CH3
G Br
Ha Br H Hb
Ha Br H G
diastereotopic
Ch. 9 - 72
diastereomers
Hb
coupling is coupling between hydrogen atoms on adjacent carbons (vicinal hydrogens), where separation between the hydrogens is by three bonds
Ha Hb
3
J or vicinal coupling
Ch. 9 - 73
coupling between heterotopic protons generally follows the n + 1 rule. Exceptions to the n + 1 rule can occur when diastereotopic hydrogens or conformationally restricted systems are involved Signal splitting is not observed for protons that are homotopic (chemical shift equivalent) or enantiotopic
Ch. 9 - 74
Hb Ha C C
Ch. 9 - 75
Splitting
Hb H a C Hb Hb H a Hb C C C C
Ch. 9 - 76
Splitting
H Hb C
H C
Hb
Ch. 9 - 77
Pascals Triangle Use to predict relative intensity of various peaks in multiplet Given by the coefficient of binomial expansion (a + b)n singlet (s) doublet (d) triplet (t) quartet (q) quintet sextet 1 11 121 1331 14641 1 5 10 10 5 1
Ch. 9 - 78
Ch. 9 - 79
Ch. 9 - 80
Karplus
curve
Ch. 9 - 81
Karplus
curve Examples
H
H Ha (axial, axial) H
b
Hb a
(equatorial, equatorial) H
a b
= 60 Ja,b = 4-5 Hz
Ch. 9 - 82
Karplus
curve Examples
Ha (equatorial, axial) Hb Ha
= 60 Ja,b = 4-5 Hz
Ch. 9 - 83
Ch. 9 - 84
The
Ch. 9 - 85
Ch. 9 - 86
The
Ch. 9 - 87
Protons of alcohols (ROH) and amines may appear over a wide range from 0.5 5.0 ppm Hydrogen-bonding is the reason for this range
R O H in conc. solution (H-bonded): R R H O O H R + H O proton more deshielded
Ch. 9 - 88
= ~0.5-1.0 ppm
Why
dont we see coupling with the OH proton, e.g. CH2OH (triplet?) Because the acidic protons are exchangeable about 105 protons per second (residence time 10-5 sec), but the NMR experiment requires a time of 10-2 10-3 sec. to take a spectrum, usually we just see an average (thus, OH protons are usually a broad singlet)
Ch. 9 - 89
Trick: Run NMR in d6-DMSO where Hbonding with DMSOs oxygen prevents Hs from exchanging and we may be able to see the coupling
Ch. 9 - 90
Deuterium
Exchange
To determine which signal in the NMR spectrum is the OH proton, shake the NMR sample with a drop of D2O and whichever peak disappears that is the OH peak (note: a new peak of HOD appears)
R O H D2O R O D + HOD
Ch. 9 - 91
Phenols
Phenol protons appear downfield at 4-7 ppm They are more acidic - more H+ character More dilute solutions - peak appears upfield: towards 4 ppm
OH O H
Ch. 9 - 92
Phenols
12.1 ppm
H O
Ch. 9 - 93
with natural abundance ~99.98%, only 1.1% of carbon, namely 13C, is NMR active
Ch. 9 - 94
NMR spectra have only become commonplace more recently with the introduction of the Fourier Transform (FT) technique, where averaging of many scans is possible (note 13C spectra are 6000 times weaker than 1H spectra, thus require a lot more scans for a good spectrum)
Ch. 9 - 95
Note
for a 200 MHz NMR (field strength 4.70 Tesla) 1H NMR Frequency = 200 MHz 13C NMR Frequency = 50 MHz
Ch. 9 - 96
Example:
2-Butanol
Ch. 9 - 97
Example:
2-Butanol
Ch. 9 - 98
electron density around an atom deshields the atom from the magnetic field and causes its signal to occur further downfield (higher ppm, to the left) in the NMR spectrum Relatively higher electron density around an atom shields the atom from the magnetic field and causes the signal to occur upfield (lower ppm, to the right) in the NMR spectrum
Ch. 9 - 99
In 1H NMR, (i) and (iii) most significant; in 13C NMR, (ii) most significant (since chemical shift range >> 1H NMR)
Ch. 9 - 100
Electronegative substituents cause downfield shift Increase in relative atomic mass of substituent causes upfield shift
Electronegativity Atomic Mass 2.8 2.7 2.2 35.5 79.9 126.9
13
X Cl Br I
123.3 ppm
71.9 ppm
5.7 ppm
Ch. 9 - 102
Ch. 9 - 104
Ch. 9 - 105
Cl
(a) CH2
(b) CH OH
(c) CH3
1-Chloro-2-propanol
Ch. 9 - 106
NMR spectrometers can differentiate among carbon atoms on the basis of the number of hydrogen atoms that are attached to each carbon In an off-resonance decoupled 13C NMR spectrum, each carbon signal is split into a multiplet of peaks, depending on how many hydrogens are attached to that carbon. An n + 1 rule applies, where n is the number of hydrogens on the carbon in question. Thus, a carbon with no hydrogens produces a singlet (n = 0), a carbon with one hydrogen produces a doublet (two peaks), a carbon with two hydrogens produces a triplet (three peaks), and a methyl group carbon produces a quartet (four peaks) Ch. 9 - 107
9 2
7 6
N O
4 5
Broadband proton-decoupled
13C
NMR
Ch. 9 - 108
NMR spectra indicate how many hydrogen atoms are bonded to each carbon, while also providing the chemical shift information contained in a broadband proton-decoupled 13C NMR spectrum. The carbon signals in a DEPT spectrum are classified as CH3, CH2, CH, or C accordingly
Ch. 9 - 109
Cl
(a) CH2
(b) CH
(c) CH3
(b)
(a)
(c)
OH 1-Chloro-2-propanol
Ch. 9 - 110
The
Ch. 9 - 111
Ch. 9 - 112
HCOSY
of 2-chloro-butane
H2 H3 H1 H4
H4 H1 H3
H2
Ch. 9 - 113
HETCOR
of 2-chloro-butane
C3 C2 C1 C4
H4 H1 H3
H2
Ch. 9 - 114
57 71
85
M+ 114
Ch. 9 - 115
The
[C8H18] + 2 e(M+)
The
largest or most abundant peak is called the base peak and is assigned an intensity of 100%, other peaks are then fractions of that e.g. 114(M+,40), 85(80), 71(60), 57(100) etc.
Ch. 9 - 116
Masses
[C8H18]
-CH3CH2CH2 (29+14)
Molecular ion (parent peak)
Ch. 9 - 117
In the mass spectrometer, a molecule in the gaseous phase under low pressure is bombarded with a beam of high-energy electrons (70 eV or ~ 1600 kcal/mol) This beam can dislodge an electron from a molecule to give a radical cation which is called the molecular ion, M+ or more accurately
M 70 eV eM
Ch. 9 - 118
This
molecular ion has considerable surplus energy so it can fly apart or fragment to give specific ions which may be diagnostic for a particular compound
- m1 A - m2 B - m3 C etc.
Ch. 9 - 119
H3C
OH
H3C
N CH3
CH3
H2C
CHCH2CH3 1-Butene
Ch. 9 - 120
Methanol
Trimethylamine
Ionization
molecules
potentials of selected
Ionization Potential (eV) 8.7 9.2 10.5 10.8 11.5 12.7
Ch. 9 - 121
16. Fragmentation
1.
2.
3.
The reactions that take place in a mass spectrometer are unimolecular, that is, they do not involve collisions between molecules or ions. This is true because the pressure is kept so low (10-6 torr) that reactions involving bimolecular collisions do not occur We use single-barbed arrows to depict mechanisms involving single electron movements The relative ion abundances, as indicated by peak intensities, are very important
Ch. 9 - 122
a molecular ion fragments, it will yield a neutral radical (not detected) and a carbocation (detected) with an even number of electrons The fragmentation will be dictated to some extent by the fragmention of the more stable carbocation:
+ ArCH2
>
+ CH2=CHCH2
>
3o
>
2o
>
1o
>
+ CH3
Ch. 9 - 123
e.g.
R+ R CH + CH3
CH3
Site of ionization:
n > >
non-bonding
Ch. 9 - 124
As
the carbon skeleton becomes more highly branched, the intensity of the molecular ion peak decreases Butane vs. isobutane
70eV ea b+ M (58)
(43)
+ CH3
(29)
+ CH2CH3
70eV e
M+(58)
(43)
+ CH3
Ch. 9 - 125
vs. isooctane
(85) + (71) +
M+(114)
(57) + (43) +
+
M+(114)
(57)
Ch. 9 - 126
Carboncarbon
bonds next to an atom with an unshared electron pair usually break readily because the resulting carbocation is resonance stabilized Ethers
Cleavage (to ether oxygen) CC bonds
O
CH3
(m/e = 59)
Ch. 9 - 128
Alcohols
CH3 +
OH (m/e = 59)
OH
M+(74)
CH3CH2 +
OH (m/e = 45)
OH
Ch. 9 - 129
Carboncarbon
bonds next to the carbonyl group of an aldehyde or ketone break readily because resonance-stabilized ions called acylium ions are produced
Ch. 9 - 130
Aldehydes
M+ peak usually observed but may be fairly weak Common fragmentation pattern -cleavage
H O R H R + H C O (m/e = 29)
Ch. 9 - 131
+ R C O acylium ion
Ketones
-cleavage
O
a b
O
(m/e = 71)
a b
(m/e = 99)
Ch. 9 - 132
Alkyl-substituted
benzenes ionize by loss of a electron and undergo loss of a hydrogen atom or methyl group to yield the relatively stable tropylium ion (see Section 14.7C). This fragmentation gives a prominent peak (sometimes the base peak) at m/z 91
Ch. 9 - 133
Aromatic
hydrocarbons very intense M+ peaks characteristic fragmentation pattern (when an alkyl group attached to the benzene ring): tropylium cation
CH3
CH3 + CH2 rearrangement tropylium cation (m/e = 91)
benzyl cation
Ch. 9 - 134
frequently show a prominent + peak at M - 18. This corresponds to the loss of a molecule of water May lose H2O by 1,2- or 1,4elimination
Ch. 9 - 135
1,2-elimination:
OH M (M - 18)
H2O
1,4-elimination: M
OH
OH + CH3CH2
(M - 18)
H2O
Ch. 9 - 136
Cycloalkenes
e.g.
+
Ch. 9 - 137
Aromatic
hydrocarbons
e.g.
Ch. 9 - 138
Ketones
McLafferty rearrangement
H O OH OH
OH
(m/e = 86)
+
Ch. 9 - 139
(m/e = 58)
OH
OH
i
H
2 radical
ii
i
ii
OH
OH
1 radical
Ch. 9 - 140
Ch. 9 - 141
not
17. How To Determine Molecular Formulas and Molecular Weights Using Mass Spectrometry
17A. Isotopic Peaks & the Molecular Ion
Ch. 9 - 143
The
presence of isotopes of carbon, hydrogen, and nitrogen in a compound + gives rise to a small M + 1 peak The presence of oxygen, sulfur, chlorine, or bromine in a compound + gives rise to an M + 2 peak
M + 1 Elements: M + 2 Elements:
C, H, N O, S, Br, Cl
Ch. 9 - 144
The
M + 1 peak can be used to determine the number of carbons in a molecule M + 2 peak can indicate whether bromine or chlorine is present isotopic peaks, in general, give us one method for determining molecular formulas
Ch. 9 - 145
+
The
The
Example
H H
1
H H
1
12 1
13 1
12
H1 M + 1 = 17
M : 16
H1 H
1
H1 H
1
H1 H
1
12
13
C12 H1
H2
H1 M : 16
H1 M + 1 = 17
100
m/z
M +1 1.17
Ch. 9 - 148
Ch. 9 - 149
Is
M odd or even? According to the nitrogen rule, if it is even, then the compound must contain an even number of nitrogen atoms (zero is an even number) For our unknown, M is even. The compound must have an even number of nitrogen atoms
+
Ch. 9 - 150
The
relative abundance of the + M +1 peak indicates the number of carbon atoms. Number of C atoms = relative + abundance of (M +1)/1.1 For our unknown
Number of C atoms =
4.5 1.1
~4
Ch. 9 - 151
The relative abundance of the M +2 peak indicates the presence (or absence) of S (4.4%), Cl (33%), or Br (98%) + For our unknown M +2 = 0.3%; thus, we can assume that S, Cl, and Br are absent The molecular formula can now be established by determining the number of hydrogen atoms and adding the appropriate number of oxygen atoms, if necessary Ch. 9 - 152
Since
M is m/z 72 molecular weight = 72 As determined using the relative + abundance of M +1 peak, number of carbons present is 4 Using the nitrogen rule, this unknown must have an even number of N. Since M.W. = 72, and there are 4 C present, (12 x 4 = 48), adding 2 N will be greater than the M.W. of the unknown. Thus, this unknown contains zero N
Ch. 9 - 153
For
For
Ch. 9 - 155
Example
O2, N2H4 and CH3OH all have M.W. of 32 (by MS), but accurate masses are different O2 = 2(15.9949) = 31.9898
Example
Both C3H8O and C2H4O2 have M.W. of 60 (by MS), but accurate masses are different
Ch. 9 - 157
Ch. 9 - 158
Ch. 9 - 159
END OF CHAPTER 9
Ch. 9 - 160