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# 5

5
-.
. . 107-115

.-. . 326-328
S () (
enzyme), , SE,
:
k

k2
1
ZZZ
X
S + E YZZ
SE

P + E
Z
k

(1)

- , .
- P.
SE:

d[SE]/dt=0,

k1 [S]*[E] = k2*[SE]+k-1*[SE]

(2)

(3)

[E]0 .

(4)
[SE]

(5)

(6)

## d[P]/dt = k2 [SE] = k2k1 [S]* [E]0/{(k2+k-1)+ k1 [S]}= r

= k2 [S]* [E]0/{(k2+k-1)/k1 + [S]}

(7)

(4), (5)

(7) [S]

[S]0.
(7) -.

(k2+k-1)/k1 = KM

(8)

. - !
r [S]

r=

[S]0:

k2 [ E ]0 [ S ]
K M + [S ]
rmax=k2[E0]

r = (1/2)*rmax

[S] = KM

[S]

[S] [S] .
:

rmax = k2 E0 [ S ]0 [ S ]  K M
[ S ]0

(9)

[ S ] .

r=

k2 [ E ]0 [ S ] k2 [ E ]0

K M  [ S ] [ S ]0
K M + [S ]
KM

(10)

[S] ,

r=

rmax k2 [ E ]0 [ S ] k2 [ E ]0
=
=
; K M = [ S ] [ S ]0
2
2[ S ]
2
(11)

- :

1
KM 1
1
1
=
+
=a
+b
r k2 [ E ]0 [ S ] k2 [ E ]0
[S ]

(12)

(12) (7).

1
1
= f

r
[S ]

1/r

tg = Km/k2[E0]

1/[S]=-1/Km

1/[S]

5
a b (12)
:

k2 =

1
a
; KM =
b[ E ]0
b

(13)

- .
, (2)
, .
:

k1[ S ][ E ] = k1[ SE ], k1  k2 ; K =

[ SE ]
k
= 1
[ S ][ E ] k1

[ E ] = [ E ]0 [ SE ],

## k1[ S ][ E ]0 k1[ S ][ SE ] = k1[ SE ],

[ SE ] =

k1[ S ][ E ]0 [ S ][ E ]0
=
,
k
k1 + k1[ S ] 1 + [ S ]
k1
r=

(14)

k2 [ S ][ E ]0
1
+ [S ]
K

,
.
- -.
`
:
k1

Agas + Cs
ACs
k 1

(15)

[ AC ]s = Kp A [C ]s

(16)
4

K=

k1
k1

(17)

AC =

[ AC ]s
Kp A
; AC =
1 + Kp A
[C ]0

(18)

, :
k2
ACs
Agas + Cs
P+C
k1

k 1

(19)

r = k2 [ AC ]s = k2 AC [C ]0 =

k2 Kp A [C ]0 k2 p A [C ]0
=
; (20)
1
1 + Kp A
+ pA
K

(20) -.
-. - , - ,
[]
[C]. - :

k2 [ S ][ E ]0
k p [C ]
k p [C ]
2 A 0= 2 A 0
1
k1
K M + [S ]
+ pA
+ pA
K
k1

(21)

- ,
.
. .
. . 117-122
5

.-. . 330-333
- , .
1) I EI :
k1
k2

SE
P + E
S + E

k1

E + I =EI

(22)

- . S I
.
2) ES, :
k2

SE
P + E
S + E
k1

k1

ES + I =ESI

(23)

.
3) , ,
:
k1
k2

SE
S + E
P + E

k1

SE + S =S2 E

(24)

.
.
(22). :
) SE ;
) EI
(. !)
) EI.

d [ SE ]
= k1[ S ][ E ] k1[ SE ] k2 [ SE ] = 0
dt

(25)

[ IE ] = K I [ E ][ I ], [ I ]0 [ I ]  [ EI ]

[ E ] = [ E ]0 [ SE ] [ IE ] =
[ E ]0 [ SE ] K I [ I ]0 [ E ]

(26)

(27)

(25)-(27) :

[E] =

[ E ]0 [ SE ]
;
1 + K I [ I ]0

k1[ S ]

[ E ]0 [ SE ]
= ( k1 + k2 )[ SE ];
1 + K I [ I ]0

k1[ S ]

[ E ]0
k1[ S ]
= [ SE ] ( k1 + k2 ) +

+
1 + K I [ I ]0
1
K
[
I
]
I
0

[ E ]0
1 + K I [ I ]0
[ S ] [ E ]0
=
[ SE ] =
;
k1[ S ]
K
K
I
S
+
+
1
[
]
[
]
M (
I
0)
( k1 + k2 ) +
1 + K I [ I ]0
k1[ S ]

rI =

k2 [ S ] [ E ]0
K M* + [ S ]

(28)

K M

K M* , ,

K M* = K M (1 + K I [ I ]0 ) > K M

(29)

, , .
.

rI = k2 [ E ]0 , [ S ]  K M*

(30)
7

r
rI

[S]

r [ S ]0 + K M (1 + K I [ I ]0 )
KM KI
=
=1+
[ I ]0
[ S ]0 + K M
[ S ]0 + K M
rI

(31)

- ,
, .
.

:

AC =

K A pA
;
1 + K A p A + K B pB

AC

- ,

(32)

AC .

r=

k2 K A p A[C ]0
k2 p A [C ]0
;
=
1
1 + K A p A + K B pB
(1 + K B pB ) + p A
KA

(33)

5
:

k2 [ S ][ E ]0
k2 p A[C ]0
;

1
K M (1 + K I [ I ]o ) + [ S ]
(1 + K B pB ) + p A
KA

(34)

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