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  • Glycerol Storage For Bacteria

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    Ray Mrinal
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    To store bacterial cultures with glycerol: add 0.15 ml of sterile glycerol to 0.85 ml of bacterial culture to make a 15% glycerol solution, vortex to mix, transfer to a labeled storage tube, freeze in dry ice or liquid nitrogen and store at -70°C. Cultures can be recovered by scraping bacteria from the frozen surface with a loop, streaking onto agar plates with antibiotics, and incubating overnight at 37°C.

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    erfwsfd

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    Glycerol Storage for Bacteria

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    To store bacterial cultures with glycerol: add 0.15 ml of sterile glycerol to 0.85 ml of bacterial culture to make a 15% glycerol solution, vortex to mix, transfer to a labeled storage tube, freeze in dry ice or liquid nitrogen and store at -70°C. Cultures can be recovered by scraping bacteria from the frozen surface with a loop, streaking onto agar plates with antibiotics, and incubating overnight at 37°C.

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    94 просмотров1 страница

    Glycerol Storage For Bacteria

    Загружено:

    Ray Mrinal
    To store bacterial cultures with glycerol: add 0.15 ml of sterile glycerol to 0.85 ml of bacterial culture to make a 15% glycerol solution, vortex to mix, transfer to a labeled storage tube, freeze in dry ice or liquid nitrogen and store at -70°C. Cultures can be recovered by scraping bacteria from the frozen surface with a loop, streaking onto agar plates with antibiotics, and incubating overnight at 37°C.

    Авторское право:

    Attribution Non-Commercial (BY-NC)
    Скачайте в формате DOC, PDF, TXT или читайте онлайн в Scribd
    Отметить как неприемлемый контент
    из 1

    Glycerol storage of bacterial cultures

    1. To 0.85 ml [3 ml] of bacterial culture, add 0.15 ml [0.53 ml] of sterile glycerol (sterilized by autoclave, final glycerol concentration : 15%, v/v). 2. Vortex the culture to ensure that the glycerol is evenly dispersed. 3. Transfer the culture to a labeled storage tube equipped with a screw cap. 4. Freeze the culture in dry ice or in liquid nitrogen, and then transfer the tube to 70 C for long-term storage. * To recover the bacteria : 1) Scrape the frozen surface of the culture with a sterile inoculating loop. 2) Immediately streak the bacteria that adhere to the loop onto the surface of an LB agar plate containing the appropriate antibiotics. 3) Return the frozen culture to storage at 70 C. 4) Incubate the plate overnight at 37 C.

    Dr. Lees Lab

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