Course No: GEB 1101, Course Name: Fundamentals of Genetic Engineering and Biotechnology Full marks: 30, Time:

2 hours, Date: 11-08-10 Answer any five

1. a. What is cloning? b. Describe the steps of cloning. c. Mention the feature of pBR322 that made it a useful cloning vector. 2. a. Define plasmid. b. discuss the desirable properties of a plasmid which will be used as a cloning vector. c. Briefly describe the various types of host cell used for genetic engineering. 3. a. classify bacteriophages based on their structure. b. What are differences between virulent and temperate phage? c. What are the reasons that have made phage lambda a good cloning vehicle? 4. a. What are the restriction endoneucleases and what are their sources? b. Write the recognition sequence for BamHI, EcoRI, HindIII and PstI. C. Explain the term restriction-modification system. 5. a. What is YAC? b. What are the applications of YAC vector? c. Show the way of cloning a piece of passenger DNA in YAC vector. 6. a. What is marker gene? b. What do you understand by the terms reporter gene and selectable marker gene and describe each type with example. c. Write down Ti-plasmid organization. 7. a. Discuss briefly the functions of nucleases, polymerase and DNA ligase. b. What is the DNA delivery method that is very important in transforming plant cells? 8. Write short notes on (any three): a. Genetic testing b. PCR technology c. Genomic library d. Electroporation e. GEP