Pre Bromalain treated pooled R1R1 cell Treated 22/1/09 Test date 22/1/09

Anti-D 73/515
Saline card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
4 4 3 1 0 0
AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 2 1 0
Exp2 4 3 3 2 0.5 0
Exp3 4 3 3 2 0.5 0

reated cells washed in cellstab

AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 2 2 0 0 0
Exp2 4 2 1 0 0 0
Exp3 4 3 1 0 0 0

Pre bromalain treated pooled rr red cells Treated 22/1/09 Test date 22/1/09
Anti-D 73/515
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Sal 0 0 0 0 0 0
AHG 0 0 0 0 0 0
 Pre Bromalain treated R1R1 pooled
Treated
cells
ate
date
26/1/09
22/1/09
test
Test
date
date
26/1/09
26/1/09

Anti-D 73/515
Saline card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 1 0 0

AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp1 4 4 2 2 1 0

reated cells washed in cellstab

AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp1 4 3 2 1 0 0

Pre bromalain treated pooled rr red

treated
cells Treated
date 26/1/09
26/1/09
tdatetest
26/01/09
Test
date
date
26/1/09
26/1/09
Anti-D 73/515
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Sal 0 0 0 0 0 0
AHG 0 0 0 0 0 0
Pre Bromalain treated R1R1 pooled Treatedcells
date 26/1/09 Test date 26/1/09
Anti-D 86 i.u.
Saline card
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 2 1 0 0 0
Exp 2 4 2 1 0 0 0
Exp 3 4 2 1 0 0 0

AHG card
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 3 2 0 0
Exp 2 4 4 3 2 0 0
Exp 3 4 3 3 1 0 0

Untreated cells in cellstab

Standard AHG technique
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 1 0 0
Exp 3 4 3 2 1 0 0

Pre Bromalain treated R1R1 pooled

Treatedcells
ate
date26/1/09
26/1/09
9 Test
test
Test
date
date
date
27/1/09
27/1/09
Anti-D lot 24311
Saline card
Dilution 1:4 1:8 1:16 1:32 1:64 1:128
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 0 0
Exp 3 4 4 3 2 0 0
AHG card
Dilution 1:4 1:8 1:16 1:32 1:64 1:128
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 1 0
Exp 3 4 4 3 3 2 0

Untreated cells in cellstab

Standard AHG technique
Dilution 1:4 1:8 1:16 1:32 1:64 1:128
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 0 0 0
Exp 3 4 3 2 1 0 0

Pre Bromalain treated R1R1 pooled

Treated
cells
date 26/1/09 Test date 26/1/09
Anti-D Lot 32372B
Saline card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 2 1 0 0
Exp 2 4 4 2 1 0 0
Exp 3 4 4 2 1 0 0

AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 4 2 1 0
Exp 2 4 4 3 2 1 0
Exp 3 4 4 4 2 1 0

Untreated cells in cellstab

Standard AHG technique
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 1 0 0
Exp 3 4 3 2 1 0 0

Pre Bromalain treated R1R1 pooled

Treated
cells
date 26/1/09 Test date 26/1/09
Anti-D Lot 101
Saline card
Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Exp 1 4 2 0 0 0 0
Exp 2 4 2 1 0 0 0
Exp 3 4 2 1 0 0 0

AHG card
Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Exp 1 4 4 2 1 0 0
Exp 2 4 4 2 0 0 0
Exp 3 4 4 2 1 0 0
Untreated cells in cellstab
Standard AHG technique
Dilution 1:50 1:100 1:200 1:400 1:800 1:1600
Exp 1 2 2 1 0 0 0
Exp 2 3 2 0 0 0 0
Exp 3 3 2 0 0 0 0

Pre Bromalain treated R1R1 pooled

Treated
cells
date 26/1/09 Test date 26/1/09
Anti-D Patient Pool A
Saline card
Dilution Neat 1:2 1:4 1:8 1:16 1:32
Exp 1 2 1 0 0 0 0
Exp 2 2 1 0 0 0 0
Exp 3 3 1 0 0 0 0

AHG card
Dilution Neat 1:2 1:4 1:8 1:16 1:32
Exp 1 4 2 1 0 0 0
Exp 2 4 2 1 0 0 0
Exp 3 4 2 1 0 0 0

Untreated cells in cellstab

Standard AHG technique
Dilution Neat 1:2 1:4 1:8 1:16 1:32
Exp 1 2 1 0 0 0 0
Exp 2 2 1 0 0 0 0
Exp 3 2 1 0 0 0 0

Pre Bromalain treated R1R1 pooled

Treated
cells
date 26/1/09 Test date 27/1/09
Anti-D Lot 102
Saline card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 3 1 0 0 0
Exp 2 4 4 1 0 0 0
Exp 3 4 3 1 0 0 0

AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 1 0 0
Exp 3 4 3 2 1 0 0

Untreated cells in cellstab

Standard AHG technique
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 3 2 1 0 0 0
Exp 2 3 2 1 0 0 0
Exp 3 3 2 0 0 0 0
Pre Bromalain treated R1R1 pooled
Treated
cells
date 26/1/09 Test date 27/1/09
Anti-D Lot 201
Saline card
Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600
Exp 1 2 0 0 0 0 0
Exp 2 2 0 0 0 0 0
Exp 3 2 0 0 0 0 0
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
3 0 0 0 0 0

AHG card
Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600
Exp 1 3 2 0 0 0 0
Exp 2 3 2 0 0 0 0
Exp 3 3 2 0 0 0 0
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
4 2 1 0 0 0
Untreated cells in cellstab
Standard AHG technique
Dilution 1:300 1:600 1:1200 1:2400 1:4800 1:9600
Exp 1 2 1 0 0 0 0
Exp 2 2 1 0 0 0 0
Exp 3 2 1 0 0 0 0
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
4 3 1 0 0 0

Pre Bromalain treated R1R1 pooled

Treated
cells
date 26/1/09 Test date 27/1/09
Anti-D Lot 202
Saline card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 2 0 0 0 0 0
Exp 2 2 0 0 0 0 0
Exp 3 2 0 0 0 0 0

AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 2 1* 0 0 0
Exp 2 4 2 1* 0 0 0
Exp 3 4 2 1* 0 0 0

Untreated cells in cellstab

Standard AHG technique
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 2 1 0 0 0 0
Exp 2 2 1 0 0 0 0
Exp 3 2 1 0 0 0 0
Pre bromalain treated cells R1R1 pool treated date 26/1/09 test date 27/1/09

Anti-D 73/515
Saline card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 3 1 0 0
Exp 2 4 3 3 1 0 0
Exp3 4 3 3 1 0 0

AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 1 0
Exp3 4 4 3 2 1 0

R1R1 cells in cellstab

Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 3 2 1 0 0
Exp 2 4 3 2 1 0 0
Exp3 4 3 2 1 0 0

Pre Bromalain treated R1R1 pooled

Treated
cells
date 26/1/09 Test###
date 27/1/09
Anti-D Lot 103
Saline card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 3 3 2 1 0 0
Exp 2 3 3 2 1 0 0
Exp 3 3 2 2 1 0 0

AHG card
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 3 2 2 1 0
Exp 2 4 3 2 2 1 0
Exp 3 4 3 2 1 0 0

Untreated cells in cellstab

Standard AHG technique
Dilution 1:100 1:200 1:400 1:800 1:1600 1:3200
Exp 1 4 4 3 1 0 0
Exp 2 4 4 3 1 0 0
Exp 3 4 3 3 1 0 0

Pre Bromalain treated R1R1 pooled

Treated
cells
date 26/1/09 Test date 29/1/09
Anti-D Lot 203
Saline card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 1 0
Exp 3 4 4 3 3 1 0

AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 4 3 2 0
Exp 2 4 4 4 2 2 0
Exp 3 4 4 3 2 1 0

Untreated cells in cellstab

Standard AHG technique
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400
Exp 1 4 4 3 2 1 0
Exp 2 4 4 3 2 0 0
Exp 3 4 4 3 2 1 0
te 27/1/09
Pooled R1R1 cells Pool B
Test date 19/2/09 Treated date 14/2/09
Dilutions made in AB serum

Anti-D 73/515
Bromalised cell AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 1:12800
Exp 1 4 3 2 2 1 0
Exp 2 4 4 2 2 1 0
Exp 3 4 4 3 2 1 0

Anti-D 86 i.u.
Bromalised cell AHG card
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 3 3 2 0
Exp 2 4 4 3 3 2 1
Exp 3 4 4 3 3 2 1

Anti-D Lot 32372B

Bromalised cell AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 2 1 0 0
Exp 2 4 4 3 2 0 0
Exp 3 4 4 3 1 0 0

Anti-D Lot 203

Bromalised cell AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 2 1 0 0
Exp 2 4 4 3 2 0 0
Exp 3 4 4 3 2 0 0
Pooled R1R1 cells Pool B
Test Date 25/2/09 Cell treated 14/2/09
very weak non specific reactions
Dilutions made in Albumin saline

Anti-D 73/515
Bromalised cell AHG card
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 3 1 1 0
Exp 2 4 4 3 1 1 0
Exp 3 4 3 3 1 1 0

Anti-D 86 i.u.
Bromalised cell AHG card
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600 1:512001:102400
Exp 1 Dilutions discarded 4 3 3 1 1 0
Exp 2 4 3 2 1 1 0
Exp 3 4 3 2 1 0 0

Anti-D Lot 32372B

Bromalised cell AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 3 3 1 0 0
Exp 2 4 3 3 1 1 0
Exp 3 4 4 3 1 1 0

Anti-D Lot 203

Bromalised cell AHG card
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 Dilutions discarded 4 4 3 2 1 0
Exp 2 4 4 3 2 1 1
Exp 3 4 4 3 2 1 0
Pooled R1R1 cells Pool B
Cells Treated 14/2/09

Dilutions made in PBSS

Anti-D 73/515
Bromalised cell AHG card Test date 19/2/09
Dilution 1:100 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 3 2 2 1
Exp 2 4 4 3 2 2 1
Exp 3 4 4 3 2 2 1
Exp 1 Dilutions made 3 2 2 1 0 0
Exp 2 and discarded 3 2 2 1 0 0
Exp 3 3 2 2 1 0 0

Anti-D 86 i.u. Test date 19/2/09

Bromalised cell AHG card Test date 19/2/09
Dilution 1:400 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 3 2 1 1
Exp 2 4 4 3 2 1 1
Exp 3 4 4 3 2 1 1

Anti-D Lot 32372B

Bromalised cell AHG card Test date 19/2/09
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 4 2 2 1
Exp 2 4 4 4 3 2 1
Exp 3 4 4 4 3 2 1
Exp 1 Dilutions made 4 3 2 1 0 0
Exp 2 and discarded 4 3 2 1 0 0
Exp 3 4 3 2 1 0 0

Anti-D Lot 203

Bromalised cell AHG card Test date 19/2/09
Dilution 1:200 1:400 1:800 1:1600 1:3200 1:6400 1:12800 1:25600
Exp 1 4 4 3 3 2 1
Exp 2 4 4 4 3 2 1
Exp 3 4 4 4 3 2 1
Exp 1 Dilutions made 4 3 2 1 0 0
Exp 2 and discarded 4 3 2 1 0 0
Exp 3 4 3 2 1 0 0