Assignment

Exercise 4: Culture Media Show all computations. Box your final answer. Write your answer on a sheet of yellow paper. Write legibly. Erasures will be given corresponding deductions. 1. What is the total volume of medium needed for 20 petri plates and 20 agar slants in big tubes? (Use the maximum volume allowed). (5 pts) 2. Compute the amount of each component of the medium if the total volume needed is 560 ml. (5 pts) Tryptone glucose yeast extract agar Yeast extract 2.5 g Tryptone 5 g Glucose 1 g Agar 20 g Distilled water 1000 ml 3. Compute the amount of 2.0 M NaOH needed to adjust the pH of 400 ml of TGYA to 9.0 if, initially, the amount of 0.10 M NaOH used in adjusting 50 ml of the said medium is 1.0 ml. (5 pts)