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Name: Ashlei Sickles Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report

Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Which of the following is true of osmosis? You correctly answered: c. It is a type of diffusion. 2. Which of the following occurs when a hypertonic solution is added to cells? You correctly answered: d. The cells shrink. 3. The variable that affects osmotic pressure is You correctly answered: a. the concentration of nondiffusing solutes. 4. The net movement of water would be into the cell in a You correctly answered: b. hypotonic solution.

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Experiment Results Predict Question: Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have? Your answer : a. increased osmotic pressure Predict Question 2: What do you think will be the pressure result of the current experimental conditions? Your answer : c. pressure above the left beaker Stop & Think Questions: Why do you think there is no pressure change? You correctly answered: b. Sodium is able to diffuse through the pores. Why do you think there was no pressure change? You correctly answered: c. There is no net movement of water because the solute concentrations are the same. Experiment Data: Run Number 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 6 6 6 7 7 7 Solute Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin Glucose MWCO 20 20 20 20 20 20 50 50 50 100 100 100 100 100 100 200 200 200 200 200 200 Start Conc. L 5.00 0.00 0.00 10.00 0.00 0.00 10.00 0.00 0.00 0.00 0.00 8.00 0.00 0.00 8.00 0.00 0.00 8.00 0.00 9.00 0.00 Pres. L 170 170 170 340 340 340 0 0 0 136 136 136 0 0 0 0 0 0 153 153 153 Start Conc. R 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 8.00 0.00 0.00 0.00 0.00 0.00 10.00 Pres. R 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Rate 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0167 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0036 0.0000 0.0000 0.0044

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Post-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. 1. If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will You correctly answered: c. double. 2. Which membrane did not allow Na+ Cl- to pass through? You correctly answered: a. 20 MWCO 3. When a solute is able to diffuse through a membrane You correctly answered: b. equilibrium is reached. 4. Water diffuses Your answer: a. against its concentration gradient. Correct answer: c. toward solutes.

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Review Sheet Results 1. Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has this effect. How well did the results compare with your prediction? Your answer: Increasing the NaCl increased the osmotic pressure within the beaker. It is because the water needs to diffuse to the higher concentration gradient to diffuse. I predicted correctly. 2. Describe one way in which osmosis is similar to simple diffusion and one way in which it is different. Your answer: Both are passive transport from high to low concentration gradients. Osmosis is different from simple diffusion because ions and molecules are transported across the membrane. However, in osmosis only solvents (water) are being transported through a selectively permeable membrane.

3. Solutes are sometimes measured in milliosmoles. Explain the statement, Water chases milliosmoles. Your answer: As the solute concentraion increases, the concentratin of water decreases. 4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO membrane in place. Explain the results. How well did the results compare with your prediction? Your answer: The 10mM glucose was able to diffuse but the 9mM albumin was not. It remained in the left beaker creating osmotic pressure of 153mmHg. The results supported my prediction.

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