Important Reactions for Enterobacteriaceae:

+
Indole + VP + Citrate = TSI A/A TDA + E. coli Klebsiella Edward Enterobacter C. diversus Serratia K. oxytoca Hafnia P. vulgaris (VIP) Y. entero @25 C Morganella Prov. (x3) Add KOH and alpha naphtol to
API (VP1 and VP2) Add Kovacs indole to API (James reagent)

E. coli E. coli Proteus Shigella Klebsiella Prov (x3) S. typhi Enterobacter M. morgani Edward Hafnia Hafnia Y. entero PDA/TDA Morganella interchangeable Y. pestis K/A Red/Yellow Add Ferric Chloride Y. entero
K/K Red/Red

Gas = Shigella S. typhi P. stuartii Y. entero Y. pestis

H2S+ Edward Salm (not typhi on API) C. freundii P. mirabilis P. vulgaris

Urea + Klebsiella Proteus Prov. rett Morganella

Gel + P. mirabilis P. vulgaris Serratia Carboxylases-LDC ODC ADH + -

ONPG + Nitrate *(API) Add Reagent 1 (Sulfanilic acid) and Reagent 2 (N,N dimethyl 1-naphthylamine) to glucose well. *If yellow (-) after the addition of Reagent 1 and 2, add zinc dust + +
(NO2 production)

(reduction to N2 gas)