Endolimax nana

Diagnostic features of trophs in wet preparations

Irregular shape with blunt pseudopods next slide Small size: usually 8-10uM similar to E. hartmanii Non-directional, sluggish motility Numerous bacteria filled vacuoles next slide One indistinct nucleus with NO peripheral chromatin = clear zone where nuclear membrane is present ball in socket appearance (best seen with permanent stain) Huge dense blot-like karyosome (best seen with permanent stain) Very difficult to differentiate from I.butschlii

Drawing of E. nana troph

Irregular shape of E. nana vs other amoeba

Endolimax nana
Diagnostic features of cysts in wet preparations Oval round shape Size 5-14uM One to four small difficult to see nuclei which are often grouped together, sometimes on one end (best seen with iodine or permanent stain) No chromatoid bars / bodies Glycogen vacuoles almost never seen

E. nana cysts

E. nana same features as wet preps

Diagnostic features of trophs in permanent stains
Small size with irregular shape blunt pseudopods Small size, usually 6-8uM similar to E. hartmanii Numerous bacteria filled vacuoles One indistinct nucleus with NO peripheral chromatin = clear zone where nuclear membrane is present ball in socket appearance (best seen with permanent stain) Huge dense blot-like karyosome (best seen with permanent stain)

E. nana trophs - trichrome

E. nana same features as wet preps

Diagnostic features of cysts in permanent stains Oval round shape Size 5-14uM One to four small difficult to see nuclei which are often grouped together, sometimes on one end (best seen with iodine or permanent stain) No chromatoid bars / bodies

E. nana cysts - trichrome

Iodamoeba butschlii
Diagnostic features of troph in wet preparations
Irregular shape with blunt pseudopods Non-directional sluggish motility Numerous bacteria filled vacuoles One indistinct nucleus (best seen with permanent stain) Huge dense karyosome vs E. nana: larger usually 12-15uM other distinguishing characteristics best seen in permanent stain

Drawing of I. butschlii troph