Plant antibiotics production through

tissue culture
• Antibiotics:
• Antibiotics are substances produced by
certain microorganisms that suppress the
growth of other microorganisms and
eventually destroy them.
• PREPARATION OF PLANT TISSUE CULTURE
MEDIUM
• Measure approximately 90% of the required
volume of the deionized-distilled water in a
flask/container of double the size of the required
volume.
• Add the dehydrated medium into the water and
stir to dissolve the medium completely. Gentle
heating of the solution may be required to bring
powder into solution
• Add desired heat stable supplements to
the medium solution.
• Add additional deionized-distilled water
to the medium solution to obtain the final
required volume.
• Set the desired pH with NaOH or HCl.
• Dispense the medium into culture
vessels
• Sterilize the medium by autoclaving at 15
psi (121°C) for appropriate time period.
Higher temperature may result in poor
cell growth.
• Add heat labile supplements after
autoclaving.
 Examples of some antibiotics
• 4-Bromo-antibiotic A23187
• Alamethicin system
Amikacin
Antibiotic, Amoxicillin
Antibiotic, Amphotericin B
• Antibiotic, Ampicillin
Antibiotic, Anisomycin
Antibiotic, Antimycotics