UNIT 7: DNA Structure & Replication DAYSHEET 83-DNA Replication Laboratory

Name: _______________________________

BIOLOGY I
Date: _______________

Bellringer: Using the terms and phrases provided below, complete the concept map showing the discovery of DNA structure Amount of base pairs DNA polymerases Double helix Deoxyribose Franklin nitrogen base phosphate group replication pyrimidines (cytosine & thymine) purine (adenine & guanine) Watson and Crick

Objective: Students will learn how DNA replicates, or makes copies of itself. Homework: HW 83 is due next class! Study for your Unit 7 Examination!!!!!

Activity 1: DNA Replication Review

DNA replication is the process of ____________________ an original strand of _________. Two enzymes are needed to turn the ________________ DNA molecule into two ________________ daughter DNA molecules. The enzyme ________________ breaks the weak bonds that hold the two strands of DNA together. These bonds are called _____________________ bonds. DNA replication cannot take place until helicase separates the two strands of DNA from one another. Each _______________ strand can now be used as a ________________ for the new strand that needs to be created. The enzyme ___________________________ can now use the base-pairing rule ( ____=____, and _____=_____) to build the complimentary strand of DNA. After base-pairing, DNA polymerase must ______________-_______________ the new DNA strands to fix base pairing mistakes. Mistakes during DNA replication could lead to __________________.

Activity 2: Caption This!

Activity 3: QuizStar Formative DNA Replication Quiz

Homework 83: Unit 7 Practice Quiz Name: ___________________________________ Fill in the answers to the following questions using this word bank: Double helix Deoxyribose Sugars Wilkins Hydrogen bonds spiral staircase ribose guanine Chargaff Covalent Bonds one polymer adenine Franklin Proteins two nucleotide thymine Watson four nitrogen base cytosine Crick

Biology I Date: __________________

1) What does DNA control the production of in cells? _______________________ 2) How many strands does DNA have? _______________________ 3) What is the shape of a DNA molecule called? _______________________________ 5) The monomers, or subunits, of DNA are called ________________________. 6) Please label the DNA Nucleotide below. a.

_______________
c.

____________

O C b._____________ C C O C

7) The base pairing rules of DNA state that cytosine will always pair with _______________________ and thymine will always pair with ___________________________. 8) If you were given the one strand of DNA below, what would the complementary strands bases be?

___ ___ ___ ___ ___ ___ ___ ___ ___

9) How do two strands of DNA stick together to form a double-stranded molecule? ____________________________________ 10) Who took the picture shown below? _____________________________________ 11) Who used this picture to determine the structure of DNA (2 scientists)? __________________________ __________________________

12) Which enzyme is responsible for base pairing nucleotides and proofreading DNA during DNA replication? a. Ligase b. Helicase c. Arginase d. DNA polymerase

13) Which enzyme begins the process of DNA replication by unzipping the parent molecule of DNA? a. Ligase b. Helicase c. Arginase d. DNA polymerase