ABX Pentra 60-C Plus Analyzer - Service Manual

Pentra 60C+
Technical Manual
P/n: RAA021B
ABX DIAGNOSTICS B.P. 7290 Rue du caduce Parc Euromdecine 34184 MONTPELLIER Cedex 04 - FRANCE
Pentra 60 C+
Notice of Liability
The information in this manual is distributed on an as is basis, without warranty. While every precaution has been taken in the preparation of the manual, ABX DIAGNOSTICS will not assume any liability to any persons or entities with respect to loss or damage, caused or alleged to be caused directly or indirectly by the instructions contained in this manual or by the computer software and hardware products described herein.
Trademarks
Microsoft and Windows are registered trademarks of Microsoft corporation. Other product names mentioned within this publication may be trademarks or registered trademarks of other companies.
Graphics
All graphics including screens and printouts, photographs are for illustrations purposes only and are not contractual.
Copyright 2004 by ABX DIAGNOSTICS

All rights reserved. No part of this book may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of ABX DIAGNOSTICS.
II
Introduction
REVISIONS
INDEX
A B
TECHNICAL NOTE SOFTWARE REVISION

RAH801A RAN038A V1.61 V2.2.9
SECTION
ALL ALL
DATE
14/04/2001 15/02/2004
This document applies to the latest higher software version. When a subsequent software version changes the information in this document, a new issue will be released.
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Pentra 60 C+
1. WARNINGS AND PRECAUTIONS
NOTE
NOTE: Emphasizes the important information especially helpful to the operator before, during or after a specific operational function.
IMPORTANT
IMPORTANT: Emphasizes an operating procedure that must be followed to avoid erroneous results.
CAUTION
CAUTION: Emphasizes an operating procedure that must be followed to avoid possible damage to the instrument or erroneous test results.
WARNING
WARNING: Flags a procedure that if not followed properly, can prove to be extremely hazardous to either the operator or the environment or both.
1.1. Warnings
User manual must be enterely read and personnel trained by ABX DIAGNOSTICS before attempting to operate instrument. The user always operates with full knowledge and appreciation of instrument warnings, alarms and flags. Always refer to labeling and ABX DIAGNOSTICS instructions in order to avoid to compromise system integrity. The ABX PENTRA 60 C+ responds to the Standards and directives named in the Declaration of Conformity added at the beginning of this manual. The reagents and accessoiries stipulated by ABX DIAGNOSTICS have been validated in accordance with the European Directive for in-vitro medical devices (98/79/CE). The use of any other reagents and accessoiries may place at risk the performance of the instrument, engaging the Users reponsability. In this case, ABX DIAGNOSTICS takes no responsability for the device nor for the results rendered. Disposal gloves, eyes protection and lab coat must be worn by the operator. Local or national regulations must be applied in all the operations. Portable/mobile should not be used in proximity All peripheral devices should be IEC compatible.
IV
Introduction
1.2. Limited garantee

The duration of guarantee is stipulated in the Sales conditions associated with the purchase of this instrument. To validate the guarantee, ensure the following is adhered to: 1 - The system is operated under the instructions of this manual. 2 - Only software or hardware specified by ABX DIAGNOSTICS is installed on the instrument. This software must be the original copyrighted version. 3 - Services and repairs are provided by an ABX DIAGNOSTICS authorized technician, using only ABX DIAGNOSTICS approved spare parts. 4 - The electrical supply of the laboratory follows the national regulations. 5 - Specimens are collected and stored in normal conditions. 6 - Reagents used are those specified in this user manual. 7 - Proper tools are used when maintenance or troubleshooting operations are performed.
CAUTION
If this instrument has been supplied to you by anyone other than ABX Diagnostics or an authorised representative, ABX Diagnostics cannot guarantee this product in terms of specification, latest revision and latest documentation. Further information may be obtained from your authorised representative.
1.3. Safety precautions, electronic and moving parts

The following parts must not be handled or checked by the user: - Electrical power supply, - Electronic components. Operator injury may occur from an electric shock. Electronic components can shock and injure the user. Do not tamper with the instrument and do not remove any components (covers, doors, panels and so on) unless otherwise instructed within this document. Danger ! The battery may explode if it is not replaced correctly ! Replace only with the same or equivalent type recommanded by the manufacturer. Dispose of used batteries according to the manufacturers instructions. Moving parts: It is strictly forbidden to disable sensors as it may cause operator injuries. Protection covers must not be opened during instrument operations.
Pentra 60 C+
1.4. Biological risks

Consider all Specimens, Reagents, Calibrators, Controls, etc that contain human blood or serum as potentially infectious! Use established, good laboratory working practices when handling specimens. Wear protective gear, Gloves, Lab coats, Safety glasses and/or Face shields, and follow other bio-safety practices as specified in OSHA Blood borne Pathogens Rule (29 CFR part 1910. 1030) or equivalent bio-safety procedures. ABX DIAGNOSTICS uses disinfectant product for instrument decontamination and highly recommends it to decontaminate your instrument.
1.5. Instrument cleaning

Instrument external cleaning
The external surfaces of the instrument must be decontaminated considering the biological environment.
WARNING
Never spill liquid on the instrument. Never use Disinfectant product* that contains alcohol.
Computer screen: Use a soft clot, slightly wet with disinfectant product*. Wipe gently the screen and dry to remove any trace of moisture. All contaminated surfaces (covers, counting assembly area...): Slightly wet a sponge with disinfectant product* and wipe the dirty surfaces. Stainless steel parts: Slightly wet a sponge with disinfectant product* and wipe the dirty surfaces. Dry with a soft cloth. *Products having the following microbiological properties: Bactericidal Fungicidal Active on Aspergillus fumigatus Active on Mycobacterium tuberculosis (B.K) Antiviral (VIH, HBV and rotavirus) Product Example validated by ABX DIAGNOSTiCS: ANIOS detergent disinfectant ; WIPANIOS ; ref: 1316.424
NOTE
Please also refer to the W.H.O (World Health Organization) guidelines: Laboratory Biosafety Manual, 2nd edition, for further information.
VI
Introduction
Instrument internal cleaning

Concentrated cleaning: Counting chambers and hydraulics parts are decontaminated by using the Concentrated cleaning function as described in 2.1.2. Concentrated cleaning, page 5-11.
Sampling probe
Sampling probe must be decontaminated as follows: 1- Prepare a solution of Sodium Hypochlorite to 100ml/l. 2- Fill a 5ml tube with this solution. 3- Run 5 analysis on bleach.
NOTE
Please also refer to the W.H.O (World Health Organization) guidelines: Laboratory Biosafety Manual, 2nd edition, for further information.
VII
Pentra 60 C+
1.6. Graphics and symbols

Switch off position Switch on position
Alternating current
Manufacturer This product conforms to the EEC Standards and Directives named in the declaration of conformity
In Vitro Diagnostics medical device
Caution, consult accompanying documents
Biological risk
Reagent
Up
Fragile, handle with care
Keep dry
Do not stack
Temperature limitation
Batch code
Catalogue number
Use by
Consult Instructions for use
Calibrator
Control
Content
VIII
Introduction
2. WORKING CONDITIONS
2.1. Environment
The operation of the ABX PENTRA 60 C+ should be restricted to indoor location use only ! Operation of the instrument at altitudes of over 3000 Meters (9800 feet) is not recommended. The instrument is designed for safety from voltages surges according to INSTALLATION CATEGORY II and POLLUTION DEGREE 2(IEC EN 61010-1). Please contact your local ABX DIAGNOSTICS representative for information regarding operation locations, when it does not comply with the recommended specifications.
2.2. Location
The ABX PENTRA 60 C+ should be placed on a clean and levelled table or workbench. Please note that the ABX PENTRA 60 C+, printer and reagents weigh approximately 40 kilograms (88 lbs). Avoid exposure to sunlight. Place your instrument where it is not exposed to water or vapor. Place your instrument where it is free from vibration or shock. Place your instrument where an independent power receptacle can be used. Use a receptacle different from the one used by a device that easily generate noise such as a centrifuge, etc... Provide a space of at least 20 cm (8 inches) at the back of the instrument for arranging the power cable and tubings. The Power switch and Input voltage supply connection should always be accessible ! When positioning the system for operational use, leave the required amount of space for easy accessibility to these items.
2.3. Grounding
Proper grounding is required when installing the system. Check the wall outlet ground (Earth) for proper grounding to the facilities electrical ground. If you are unsure of the outlet grounding, contact your facilities engineer to verify the proper outlet ground !
2.4. Humidity and temperature conditions

ABX PENTRA 60 C+ must operate between temperatures of 16 to 34C (61 to 93F). Maximum relative humidity should be 80% for temperatures up to 31C (88F) and decreasing linearly to 50% relative humidity at 40C (104F). If the system is kept at a temperature of 10C (50F) or less, it must be allowed to sit at room temperature for 1 hour before it can be used for operation.
IX
Pentra 60 C+
2.5. Electromagnetic environment check

The ABX PENTRA 60 C+ has been designed to produce less than the accepted level of electromagnetic interference in order to operate in conformity with its destination, allowing the correct operation of other instruments also in conformity with their destination. In case of suspected electromagnetic noise, check that the instrument has not been placed in the proximity of electromagnetic fields or short wave emissions, i. e. (Radar, X-rays, Scanners, Cell phones, etc...).
2.6. Environment protection

Disposal Used accessories and consumables must be collected by a laboratory specialized in elimination and recycling of this kind of material according to the local legislation. Disposal ABX PENTRA 60 C+ instrument: It should be disposed of, in accordance with local legislation, and should be treated as being contaminated with blood. The appropriate biological precautions should be taken.
2.7. Transport and storage conditions

Storage temperature: -20C +50C
Prior to the shipping of an instrument by transporter, whatever the destination, an external decontamination of the instrument must be carried out.
Introduction
INTRODUCTION
REVISIONS ........................................................................................................ III 1. WARNINGS AND PRECAUTIONS .................................................................. IV 1.1. Warnings ................................................................................................ IV 1.2. Limited garantee .................................................................................... V 1.3. Safety precautions, electronic and moving parts ................................... V 1.4. Biological risks ....................................................................................... VI 1.5. Instrument cleaning ............................................................................... VI 1.6. Graphics and symbols ......................................................................... VIII 2. WORKING CONDITIONS .............................................................................. IX 2.2. Location ................................................................................................ IX 2.3. Grounding ............................................................................................. IX 2.1. Environment .......................................................................................... IX 2.4. Humidity and temperature conditions ................................................... IX 2.5. Electromagnetic environment check ..................................................... X 2.6. Environment protection ......................................................................... X 2.7. Transport and storage conditions .......................................................... X SPECIFICATIONS ............................................................................................. XII HYDRAULIC & PNEUMATIC PRINCIPLES ........................................................XIII ELECTRIC & ELECTRONIC PRINCIPLES ......................................................... XIV ANALYSIS CYCLE TECHNOLOGY ................................................................... XV SOFTWARE VERSIONS ................................................................................... XVI OUTPUT FORMAT ......................................................................................... XVII ALARMS & ERROR LIST ............................................................................... XVIII MAINTENANCE .............................................................................................. XIX EXPLODED VIEWS .............................................................. XX
SPARE PART LIST .......................................................................................... XXI
XI
Pentra 60 C+
SPECIFICATIONS
1. TECHNICAL SPECIFICATIONS .................................................................... 1-2 1.1. Parameters .......................................................................................... 1-2
1.1.1. CBC Mode .................................................................................................. 1-2 1.1.2. CBC + 5DIFF Mode ..................................................................................... 1-3
1.1.3. Units .................................................................................................. 1.2. Throughput Analyses .......................................................................... 1.3. Tube Identification ............................................................................... 1.4. Reagents ............................................................................................. 1.5. Workstation Computer ........................................................................ 1.6. Measurements and computation ........................................................ 2. PHYSICAL SPECIFICATIONS ...................................................................... 2.1. Power requirements ............................................................................ 2.2. Operating temperature and humidity .................................................. 2.3. Dimensions and Weight ...................................................................... 2.4. Minimum specimen volume ................................................................ 2.5. Dilution ratios ...................................................................................... 2.6. Hgb measurement ............................................................................. 2.7. Counting aperture diameters .............................................................. 2.8. Reagent consumption (ml) .................................................................
1-4 1-5 1-5 1-5 1-5 1-5 1-6 1-6 1-6 1-6 1-6 1-7 1-7 1-7 1-8
3. SUMMARY OF PERFORMANCE DATA ....................................................... 1-9 3.1. Precision (Reproducibility)* .................................................................. 1-9 3.2. Precision (Repeatability) .................................................................... 1-10
Precision claims* ............................................................................................... 1-10
3.2. Linearity* ............................................................................................. 1-11 3.3. Carryover* .......................................................................................... 1-11 3.4. Normal ranges* ................................................................................. 1-12 3.5. Accuracy* .......................................................................................... 1-13 3.6. Leukocyte differential count ............................................................... 1-13 3.7. Sample stability study ........................................................................ 1-13 3.8. Flags giving an analysis default .......................................................... 1-14 4. REAGENTS SPECIFICATIONS .................................................................... 1-15 4.1. Reagent specifications ....................................................................... 1-15 4.2. Waste handling precautions .............................................................. 1-15 5. LIMITATIONS ............................................................................................. 1-16 5.1. Maintenance ...................................................................................... 1-16 5.2. Blood specimens ............................................................................... 1-16 5.3. Known interfering substances ........................................................... 1-17
XII
Introduction
HYDRAULIC & PNEUMATIC PRINCIPLES
1. HYDROPNEUMATIC CONNECTIONS .......................................................... 2-2 2. FUNCTION OF VALVES .............................................................................. 2-5 3. VALVES LOCATIONS .................................................................................. 2-6 4. PNEUMATIC DIAGRAM .............................................................................. 2-7
XIII
Pentra 60 C+
ELECTRIC & ELECTRONIC PRINCIPLES
1. MAIN BOARD TEST POINTS ....................................................................... 3-2 2. MAIN BOARD POTENTIOMETERS .............................................................. 3-4 3. PENTRA 60 C+ BOARDS ............................................................................ 3-5 3.1 Main board XAA 425 C ......................................................................... 3-5 3.2. Optical Preampli board XAA 423 B ..................................................... 3-6 3.3. Motor relay board XAA 427 B ............................................................. 3-7 3.4. Carriage board XAA 428 C ................................................................. 3-8 3.5. LED board XAA 429 A ......................................................................... 3-9 4. PENTRA 60 C+ CONNECTORS ................................................................ 3-10 4.1. Chamber Heating Connector XBA 386 A .......................................... 3-10 4.2. Reagent Heating Coil Connector XBA 387 A ..................................... 3-11 4.3. Horizontal Carriage Motor XBA 391 A ............................................... 3-12 4.4. Upper Fan XBA 393 A ....................................................................... 3-13 4.5. IR sensor XBA 394 AS ...................................................................... 3-14 4.6. IR sensor XBA 395 AS ...................................................................... 3-15 4.7. IR sensor XBA 396 AS ....................................................................... 3-16 4.8. IR sensor XBA 397 AS ....................................................................... 3-17 4.9. RBC / WBC coaxe XBA 398 B .......................................................... 3-18 4.10. LMNE flowcell coaxe XBA 399 AS ................................................... 3-19 4.11. Barcode reader cable XBA 402 AS .................................................. 3-20 4.12. Diluent level sensor XDA 605 AS ..................................................... 3-21 5. PENTRA 60 C+ SYNOPTIC ....................................................................... 3-22
XIV
Introduction
ANALYSIS CYCLE TECHNOLOGY
1. ANALYSIS CYCLE DESCRIPTION ................................................................ 2-2 2. MEASURING PRINCIPLES .......................................................................... 2-4 2.1. Multi Distribution Sampling System (MDSS) ......................................... 2-4 2.2. RBC / Plt detection principles ............................................................. 2-5 2.3. Hgb measurement ............................................................................. 2-6 2.4. Hct measurement ............................................................................... 2-7 2.5. RDW calculation .................................................................................. 2-7 2.6. MCV, MCH, MCHC calculation ............................................................ 2-7 2.7. MPV Measurement ............................................................................. 2-8 2.8. Pct calculation .................................................................................... 2-8 2.9. PDW calculation .................................................................................. 2-8 2.10. WBC and differential count ................................................................ 2-9
2.10.1. General principles .................................................................................... 2-9 2.10.2. BASO / WBC Count ................................................................................ 2-9 2.10.3. LMNE matrix .......................................................................................... 2-10
XV
Pentra 60 C+
SOFTWARE VERSIONS
1. SERVICE SOFTWARE OVERVIEW ............................................................... 5-2 2. SOFTWARE RELEASES .............................................................................. 5-3 2.1. Software releases table ....................................................................... 5-3 2.2. Software modifications ....................................................................... 5-3
XVI
Introduction
OUTPUT FORMAT
Refer to document ABX Diagnostics OUTPUT FORMAT RAA024 7-1
XVII
Pentra 60 C+
ALARMS & ERROR LIST
1. TRANSMISSION .......................................................................................... 7-2 2. DATABASE ................................................................................................. 7-2 3. WORKLIST ................................................................................................. 7-2 4. CALIBRATION ............................................................................................ 7-3 5. TECHNICAL OPERATIONS ......................................................................... 7-3 6. PRINTER .................................................................................................... 7-3
XVIII
Introduction
MAINTENANCE
1. MAINTENANCE .......................................................................................... 8-2 1.1. Introduction .......................................................................................... 8-2 1.2. Daily customer maintenance ............................................................... 8-2 1.3. Weekly customer maintenance ........................................................... 8-2 1.4. Maintenance table ............................................................................... 8-3 2. MAINTENANCE KITS .................................................................................. 8-4 2.1. 6 month maintenance kit ..................................................................... 8-4 2.2. Yearly maintenance kit ........................................................................ 8-4 2.3. Pistons maintenance kit ...................................................................... 8-5 2.4. Fitting kit ............................................................................................. 8-5 2.5. Screws kit ........................................................................................... 8-6 3. PROCEDURES ............................................................................................ 8-7 3.1. Required tools and product ................................................................. 8-7 3.2. Procedure chart table ......................................................................... 8-8 3.3. Procedure chart table in maintenance order ...................................... 8-9
XIX
Pentra 60 C+
EXPLODED VIEWS
XX
Introduction
SPARE PART LIST
SPARE PART LIST ......................................................................................... 11-2
XXI
Pentra 60 C+
SPECIFICATIONS
1. TECHNICAL SPECIFICATIONS ................................................. 1-2 1.1. Parameters ....................................................................... 1-2 1.1.1. CBC Mode ................................................................ 1-2 1.1.2. CBC + 5DIFF Mode ................................................... 1-3 1.1.3. Units .............................................................................. 1-4 1.2. Throughput Analyses ....................................................... 1-5 1.3. Tube Identification ............................................................ 1-5 1.4. Reagents ......................................................................... 1-5 1.5. Workstation Computer ..................................................... 1-5 1.6. Measurements and computation ..................................... 1-5 2. PHYSICAL SPECIFICATIONS ................................................... 1-6 2.1. Power requirements ........................................................ 1-6 2.2. Operating temperature and humidity .............................. 1-6 2.3. Dimensions and Weight .................................................. 1-6 2.4. Minimum specimen volume ............................................ 1-6 2.5. Dilution ratios .................................................................. 1-7 2.6. Hgb measurement .......................................................... 1-7 2.7. Counting aperture diameters ........................................... 1-7 2.8. Reagent consumption (ml) .............................................. 1-8 3. SUMMARY OF PERFORMANCE DATA .................................... 1-9 3.1. Precision (Reproducibility)* .............................................. 1-9 3.2. Precision (Repeatability) ................................................. 1-10 Precision claims* .............................................................. 1-10 3.2. Linearity* ........................................................................ 1-11 3.3. Carryover* ...................................................................... 1-11 3.4. Normal ranges* .............................................................. 1-12 3.5. Accuracy* ...................................................................... 1-13 3.6. Leukocyte differential count ........................................... 1-13 3.7. Sample stability study ..................................................... 1-13 3.8. Flags giving an analysis default ...................................... 1-14 4. REAGENTS SPECIFICATIONS ................................................ 1-15 4.1. Reagent specifications .................................................... 1-15 4.2. Waste handling precautions ........................................... 1-15 5. LIMITATIONS .......................................................................... 1-16 5.1. Maintenance ................................................................... 1-16 5.2. Blood specimens ........................................................... 1-16 5.3. Known interfering substances ........................................ 1-17
Pentra 60 C+
1. TECHNICAL SPECIFICATIONS
The ABX PENTRA 60 C+ is a fully automated hematology analyzer used for in vitro diagnostic testing of whole blood specimens. A control station (or workstation) is directly connected to the instrument. The ABX PENTRA 60 C+ is able to operate either in CBC mode (Cell Blood Count:12 parameters) or in CBC + 5DIFF mode (5 population Differential count: 26 parameters).
1.1. Parameters
1.1.1. CBC Mode
WBC RBC Hgb Hct MCV MCH MCHC RDW Plt PDW * MPV Pct * White Blood Cell Red Blood Cell Hemoglobin concentration Hematocrit Mean Corpuscular Volume Mean Corpuscular Hemoglobin Mean Corpuscular Hemoglobin Concentration Red Distribution Width Platelets Platelet Distribution Width Mean Platelet Volume Plateletcrit
* PDW and PCT have not been established as indications for this product,in the United States. The use of PCT and PDW should be restricted to research and Investigational measurements only.
1-2
Specifications
1.1.2. CBC + 5DIFF Mode

WBC LYM MON NEU EOS BAS LIC * ALY * RBC Hgb Hct MCV MCH MCHC RDW Plt PDW * MPV Pct * White Blood Cell Lymphocytes % and # Monocytes % and # Neutrophils % and # Eosinophils % and # Basophils % and # Large Immature Cell % and # Atypical Lymphocyte % and # Red Blood Cell Hemoglobin concentration Hematocrit Mean Corpuscular Volume Mean Corpuscular Hemoglobin Mean Corpuscular Hemoglobin Concentration Red Distribution Width Platelets Platelet Distribution Width Mean Platelet Volume Plateletcrit
* PCT, PDW, ALY and LIC have not been established as indications for this product,in the United States. The use of PCT, PDW, ALY and LIC should be restricted to research and Investigational measurements only.
1-3
Pentra 60 C+
1.1.3. Units
UNITS
STD SI mmol/l JAPAN
WBC 103/mm3 109/l 109/l 102/mm3 MCH pg pg fmol pg LYC % % % % EOS # # # #
RBC 106/mm3 1012/l 1012/l 104/mm3 MCHC g/dl g/l mmol/l g/dl LYC # # # # BAS % % % %
HGB g/dl g/l mmol/l g/dl RDW % % % % MON % % % % BAS # # # #
HCT % l/l l/l % MPV m3 fl fl m3 MON # # # # ALY % % % %
PLT 103/mm3 109/l 109/l 104/mm3 PCT % 10-2/l 10-2/l % NEU % % % % ALY # # # #
MCV m3 fl fl m3 PDW % % % % NEU # # # # LIC % % % %
STD SI mmol/l JAPAN STD SI mmol/l JAPAN
EOS % % % % LIC # # # #
STD SI mmol/l JAPAN
To select the set of units refers to Chapter 4. Instrument configuration
1-4
Specifications
1.2. Throughput Analyses

CBC Mode (CBC): .............. 60/h CBC + 5DIFF Mode (DIF): .. 60/h
1.3. Tube Identification

By means of the ABX WORKSTATION: Barcode labels or keyboard.
1.4. Reagents
ABX DILUENT (20 litres) ABX CLEANER (1 litre, Integrated), ABX EOSINOFIX (1litre, Integrated), ABX BASOLYSE II (1 litre, Integrated), ABX ALPHALYSE or BIOLYSE (0.4 litre, Integrated)
1.5. Workstation Computer

Processor frequency: ......... Memory capacity: .............. Hard drive: .......................... Floppy + CD-ROM Keyboard: ........................... Mouse: ............................... RS232 port: ........................ Screen: ............................... Operating System: ............. * Pentium II 350 Mhz (min.) * 128 Mb (min.) * 4 Gb (min.) * 105 keys (Qwerty default or Option) * Standard PS/2 compatible * x2 (Analyser/Local network) * 15 monitor (800x600 min.) * Microsoft Windows NT 4.0 Service pack 4 GB or US
1.6. Measurements and computation

Impedance for WBC, Plt, RBC, BASO. Photometry for Hgb. Impedance and light scattering for LYM, MON, NEU, EOS, ALY and LIC. Computation from stored data that was directly measured for Hct, MCV, MCH, MCHC, RDW, MPV, Pct, PDW.
1-5
Pentra 60 C+
2. PHYSICAL SPECIFICATIONS
2.1. Power requirements

Power supplies: ................. * from 100Vac to 240Vac (+10%) .................................. * 50 Hz to 60 Hz Power Consumption: ......... * Analyzer and computer: 400 VA .................................. * Printer (Depends on printer see Printers manual) .................................. * EPSON EPL5900: 900 VA
2.2. Operating temperature and humidity

16 - 34C (61 - 93F) room temperature Maximum relative humidity 80% for temperatures up to 31C (88F) decreasing linearly to 50% relative humidity at 40C (104F).
2.3. Dimensions and Weight

Analyzer dimensions: ......... .................................. .................................. Analyzer weight: ................. * Height: approximately 516 mm (20.3 in.) * Width: approximately 444 mm (17.5 in.) * Depth: approximately 481 mm (19 in.) * approximately 35Kgs (77lbs)
Computer dimensions: ....... * See computers manual Computer weight: .............. * See computers manual
2.4. Minimum specimen volume

CBC Mode (CBC): .............. * 30l CBC + 5DIFF Mode (DIF): .. * 53l
1-6
Specifications
2.5. Dilution ratios

WBC/BASO ........................ LMNE ................................. RBC/Plt .............................. Hgb .................................. * 1/200 * 1/80 * 1/10 000 * 1/250
2.6. Hgb measurement

Hgb chamber, LED 555 nm. Modified Drabkin method (cyanmethemoglobin) Light source ....................... * Electroluminescent diode Wavelength ........................ * 550nm + 10nm
2.7. Counting aperture diameters

WBC/BASO ........................ * 80 m LMNE ................................. * 60 m RBC/Plt .............................. * 50 m
1-7
Pentra 60 C+
2.8. Reagent consumption (ml)

CYCLE
DILUENT BASOLYSE II
REAGENT
CLEANER EOSINOFIX ALPHALYSE
APPROXIMATE DURATION
CBC (CBC) 22.6 5DIFF (DIF) 28.5 Startup* 65.4 Shutdown 27 Diluent Prime 44.9 Cleaner Prime X Eosinofix Prime X Basolyse II Prime X Alphalyse Prime 2.1 All Reagents Prime 49 Autoclean 13.4 Autocontrol 25.4
2.1 2.1 2.1 X X X X 23.6 X 24 1 X
0.9 0.9 3.7 14 X 24.8 X 1.1 X 25.1 1 1.4
X 1 1 X X X 23.6 X X 24 1 X
0.4 0.4 1.4 1 X X X X 8.4 8.2 0.3 1
60 60 450 300 310 120 110 220 130 6 135 150
* for one background count only (maxi = 3)
1-8
Specifications
3. SUMMARY OF PERFORMANCE DATA
3.1. Precision (Reproducibility)*
The ABX PENTRA 60 C+ was initially calibrated with Minocal Calibrator (Lot No. CX322, Expiry Date: 05-Aug-2002). Three levels of ABX MINOTROL material (Lot No: JX108) were run in duplicate once daily for a prolonged period (26th July 2002 to 30 August 2002) on all parameters. The results were used to quantify within run precision, and Total Precision in accordance with the NCCLS EP 5-A Guidelines.
PARAMETER ABX MINOTROL CONTROL WITHIN RUN SD SD OF RUN
MEANS
SD OF
MEANS
DAILY
TOTAL PRECISION (SD)
WBC
RBC
HGB
HCT
PLT
PARAMETER
JX108 Low JX108 Normal JX108 High JX108 Low JX108 Normal JX108 High JX108 Low JX108 Normal JX108 High JX108 Low JX108 Normal JX108 High JX108 Low JX108 Normal JX108 High
ABX MINOTROL CONTROL
0.001 0.008 0.031 0.000 0.001 0.002 0.001 0.002 0.005 0.021 0.064 0.104 6.271 40.229 154.146
WITHIN RUN CV%
N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
CV% OF RUN
MEANS
CV% OF DAILY
MEANS
0.001 0.013 0.045 0.000 0.001 0.003 0.001 0.007 0.012 0.025 0.122 0.181 20.646 72.103 381.388
TOTAL PRECISION (CV%)
WBC
RBC
HGB
HCT
PLT
JX108 Low JX108 Normal JX108 High JX108 Low JX108 Normal JX108 High JX108 Low JX108 Normal JX108 High JX108 Low JX108 Normal JX108 High JX108 Low JX108 Normal JX108 High
1.8 0.9 0.9 0.8 0.6 0.7 0.4 0.3 0.4 0.9 0.7 0.7 3.1 2.6 2.5
1.93 1.12 1.05 0.86 0.79 0.88 0.57 0.59 0.60 0.99 0.97 0.89 5.69 3.46 4.01
*Source: 510K submission K030144

1-9
Pentra 60 C+
Precision claims*
PARAMETER % CV NOMINAL VALUES
WBC RBC HGB HCT PLT
< 2.0% < 2.0% < 1.0% < 2.0% < 5.0%
10.0 x 103/mL 4.67 x 106/mL 13.6 g/dl 36.0 % 243 x 103/mL
3.2. Precision (Repeatability)

Based on 10 consecutive samples of the same fresh whole blood sample without alarm:
PARAMETERS
%CV
TEST
LEVEL
WBC RBC Plt Hgb Hct
<2% <2% <5% <1% <2%
at 10x103/L at 5x106/L at 300x103/L at 15 g/dL at 45%
1-10
Specifications
3.2. Linearity*
Linearity range: The Manufacturers tested linearity zone of the instrument using linearity kits and/or human blood. Linearity limits: Maximum and minimum values within instrument returns no dilution alarm. Visible range: Range values given by the instrument. These values (above linearity limits) are given as an indication. They are given associated with a D flag. This Visible range is outside Manufacturers range. Linearity kits Linearity was tested using available Low Range and Full Range Linearity Test kits. The Test kits were analyzed and data was computed according to the Manufacturers instructions. Human Blood Linearity was also obtained on human blood, using a minimum of 5 dilution points. The re-sults of this study are as followed:
PARAMETER LINEARITY
RANGE
LINEARITY
LIMITS
VISIBLE
RANGE
DIFFERENCE
WBC (103/L) RBC (106/L) HGB (g/dL) HCT (%) PLT (103/L) PLT (103/L)
6
0.40 130.80 0.23 9.76 0.00 31.06 1.80 88.90 3.30 2007 7.00 - 2895
0 120 0 8.00 0 24 0 67 0 1900 0 2800
120 150 8.00 18.00 24 30 67 80 1900 2800 2800 3200
0.3 7% 0.07 2% 0.3 2% 2.0 3% 10 10% 10 10%
(Hgb>2g/dL,RBC>0.5x106/L)
(Hgb<2g/dL,RBC<0.5x10 /L)
3.3. Carryover*
The ABX PENTRA 60 C+ carry-over effects were evaluated by assaying a sample with high cell concentrations three consecutive times (i1-3), followed immediately by testing a diluted sample consecutively 3 times (j1-3). (j1-j3) Carryover = (i3-j3) Carry-over % is then:
WBC RBC HGB PLT
X 100
Mean low levels Mean high levels Carry over %
1.06 58.81 -0.260
1.58 6.37 0.000
5.28 22.03 -0.179
31.33 1106.67 -0.186
This is the method as described in Guidelines for the Evaluation of blood cell analyzers including those used for differential leukocyte and reticulocyte counting and cell marker applications. ISLH, 14 January, 1994.
1-11
Pentra 60 C+
Carry-over Conclusion: Results provided are extremely satisfactory. In order to provide for eventual possibilities within the laboratory environment the following claims shall be made :
WBC RBC HGB PLT
Claims
< 2.0%
< 2.0%
< 2.0%
< 2.0%
3.4. Normal ranges*

PARAMETERS MALE FEMALE
WBC (103/L) RBC (106/L) HGB (g/dL) HCT (%) MCV (m3) MCH (pg) MCHC (g/dL) RDW (%) PLT (103/L) MPV (m3) PCT (%) PDW (%) NEU (%) LYM (%) MON (%) EOS (%) BAS (%)
4 - 10 4.50 - 6.50 13.0 - 17.0 40.0 - 54.0 80 - 100 27.0 - 32.0 32.0 - 36.0 11.0 - 16.0 150 - 500 6 - 11 0.15 - 0.50 11 - 18 50 - 80 25 - 50 2 - 10 0-5 0-2
4 - 10 3.80 - 5.80 11.5 - 16.0 37.0 - 47.0 80 - 100 27.0 - 32.0 32.0 - 36.0 11.0 - 16.0 150 - 500 6 - 11 0.15 - 0.50 11 - 18 50 - 80 25 - 50 2 - 10 0-5 0-2
IMPORTANT
Expected values will vary with sample population and/or geographical location. It is highly recommended that each Laboratory establish its own Normal ranges based upon the local population!
*Bibliography: AIDE MEMOIRE DHEMATOLOGIE Prof : C.SULTAN / M. GOUAULT- HELMANN / M. IMBERT Service Central dHmatologie de lHopital Henri Mondor Facult de mdecine de Crteil (Paris XII)
1-12
Specifications
3.5. Accuracy*
The data shows good correlation between results achieved on the ABX PENTRA 60 C+ versus the reference system, which can be resumed as follows:
PARAMETER R(COMPARISON OF
MEANS)
ACCURACY CLAIMS
WBC PLT RBC HGB HCT LYM NEU MON EOS BAS
0.997 0.998 N/A N/A N/A N/A N/A N/A N/A N/A
>0.95 >0.95 N/A N/A N/A N/A N/A N/A N/A N/A
3.6. Leukocyte differential count

Not available at the time of publication.
3.7. Sample stability study

Not available at the time of publication.
1-13
Pentra 60 C+
3.8. Flags giving an analysis default
Results displayed
PARAMETER LINEARITY
LIMITS
VISIBLE
RANGE
>VISIBLE
RANGE
WBC(x103/mm3) RBC(x106 /mm3) PLT(x103/mm3) HGB(g/dL) HCT(%)
HGB>2g/dL HGB<2g/dL
result result result result result result
result+ D result+ D result+ D result+ D result+ D result+ D
DIL DIL DIL DIL DIL DIL
Results transmitted and printed out

PARAMETER LINEARITY
LIMITS
VISIBLE
RANGE
>VISIBLE
RANGE
WBC(x103/mm3) RBC(x106 /mm3) PLT(x103/mm3) HGB(g/dL) HCT(%)
HGB>2g/dL HGB<2g/dL
result result result result result result
result+D result+D result+D result+D result+ D result+ D
--.-- + D --.-- + D --.-- + D --.-- + D --.-- + D --.-- + D
NOTE
Use the instrument diluent to dilute the sample if a ---- D flag occurs on WBC or Hct.
1-14
Specifications
4. REAGENTS SPECIFICATIONS
4.1. Reagent specifications

In order for the instrument to operate correctly, high-quality reagents must be used. ABX DIAGNOSTICS provides all the necessary reagents.
NOTE
The ABX Diagnostics reagents specified for this instrument may have followed one or both of the approval methods below: 1 - have been registered by the A.F.S.S.A.P.S. Agence Franaise de Scurit Sanitaire des Produits de Sant according to the procedure relative to laboratory reagents used for biological analyses. 2 - or approved in accordance with the European Directive 98/79/CE (Annex III) for in-vitro medical devices. Please refer to the packaging of each reagent concerned to establish the approval method.
Refer to Chapter Annex of this manual for all reagent speifications.
4.2. Waste handling precautions
WARNING
When disposing of waste, protective clothing must be worn (lab coat, gloves, eye protection, etc). Follow your local and /or national guidelines for biohazard waste disposal.
If required, waste can be neutralized before being discarded. Follow your laboratorys protocol when neutralizing and disposing of waste. Dispose of the waste container according to the local or national regulatory requirements.
1-15
Pentra 60 C+
5. LIMITATIONS
WARNING
Whilst every effort is taken by ABX Diagnostics to investigate and indicate all known interferences, it is by no means possible to guarantee that all interferences have been identified. At all times, results should be validated and communicated only once all information relating to the patient has been assessed and taken into account.
5.1. Maintenance
In Chapter 5. Maintenance & Troubleshooting, specific maintenance procedures are listed. The maintenance procedures identified are mandatory for proper use and operation of the ABX PENTRA 60 C+. Failure to execute any of these recommended procedures may result in poor reliability of the system.
IMPORTANT
Failure to execute any of these recommended procedures may result in poor reliability of the system.
5.2. Blood specimens

Verification of any abnormal test result (including flagged results or results outside of the normal range) should be performed using reference methods or other standard laboratory procedures for conclusive verification of the results. The sections below list known limitations of automated blood cell counters which use the principles of impedance and light absorbance as principles of measurement.
1-16
Specifications
5.3. Known interfering substances

White Blood Cells (Leukocytes):
WBC results that exceed the linearity limits of the system will require dilution of the blood sample (Leukemia sample followed by a leukopenia). Re-assaying the diluted sample will help to obtain the correct assay value. Unlysed Red Cells - In some rare instances, the erythrocytes in the blood sample may not be completely lysed. These non-lysed red blood cells may be detected on the WBC histogram with an L1 alarm or as an elevated baseline on the side (leading edge) of the lymphocytes population. Non-lysed erythrocytes will cause a falsely elevated WBC count. Multiple myeloma - The precipitation of proteins in multiple myeloma patients may give high WBC counts. Leukemia - A very low WBC count may result from this disease because of possible increased fragility of the leukocytes leading to destruction of some of these cells during counting. These white cell fragments will also interfere with the white cell differential parameters. These white cell fragments will also interfere with the white cell partial differential parameters: LYM% + #, MON% + #, GRAN% + #. A suspiciously low WBC count may also be seen in patients with lymphocytic leukemias due to the presence of abnormally small lymphocytes which may not be counted by the instrument. Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of the leukocytes which may cause low WBC counts. Cryoglobulins - Increased levels of cryoglobulin that may be associated with myeloma, carcinoma, leukemia, macroglobulinemia, lymphoproliferative disorders, metastic tumors, autoimmune disorders, infections, aneurism, pregnancy, thromboembolic phenomena, diabetes, etc, which can increase the WBC, RBC or PLT counts and the HGB concentration. The specimen must be warmed up to 37C (99F) in a bain marie for 30 minutes and analyzed again immediately after (analyzer or manual method). Macrothrombocytes - in excessive numbers may affect and increase Leukocyte numeration.
RBC: Red Blood Cells (Erythrocytes)

The red blood cell dilution contains all the formed elements in the blood: erythrocytes, leukocytes and platelets. During erythrocytes counting (red blood cells), platelets are not counted as their size falls below the minimum threshold. Agglutinated erythrocytes - May cause a low incorrect RBC count. Blood samples containing the agglutinated red blood cells may be suspected by elevated MCH and MCHC values and shown by examination of the stained blood film. Cold agglutinins - IgM immunoglobulins which are high in cold agglutinin disease may cause lower RBC and PLT counts and increase MCV.
1-17
Pentra 60 C+
Hgb (Hemoglobin):
Turbidity of the blood sample - Any number of physiological and/or therapeutic factors may produce high incorrect HGB results. To obtain accurate hemoglobin results when increased turbidity of the blood sample occurs, determine the cause of the turbidity and follow the appropriate method below: High WBC: An extremely high WBC will cause excessive light scatter. In these cases use reference (manual) methods.The diluted sample should be centrifuged, and the supernatant fluid measured with a spectrophotometer. High lipid concentration: A high concentration of lipids in the blood sample will give the plasma a milky appearance. This condition can occur with hyperlipidemia, hyperproteinemia (as in gammapathies) and hyperbilirubinemia. Accurate hemoglobin determinations can be achieved by using reference (manual) methods and a plasma blank. Increased turbidity may also be seen in cases where the red blood cells are resistant to lysing. This condition will cause an incorrect high HGB result, but may be detected by observing the abnormal MCH, MCHC values, and the increased baseline on the leading edge of the WBC histogram. Erroneous hemoglobin results will cause the results of the MCH and MCHC to be incorrect as well. Fetal bloods - The mixing of fetal and maternal bloods may produce a high inaccurate HGB value.
Hct (Hematocrit)
Red blood cells agglutination - May produce an inaccurate HCT and MCV values. Red blood cell agglutination may be detected by observing abnormal MCH and MCHC values, as well as by examination of the stained blood film In such cases, manual methods may be required to obtain an accurate HCT value.
MCV (Mean Corpuscular Volume)

Red blood cell agglutination - May produce an inaccurate MCV value. Red blood cell agglutination may be detected by observing abnormal MCH and MCHC values, as well as by examination of the stained blood film. In such cases, manual methods may be required to obtain an accurate MCV value. Excessive numbers of large platelets and/or the presence of an excessively high WBC count may interfere with the accurate determination of the MCV value. In such cases, careful examination of the stained blood film may reveal the error.
MCH (Mean Corpuscular Hemoglobin)

The MCH is determined according to HGB value and the RBC count. The limitations listed for the HGB and RBC will have an effect on the MCH and may cause inaccurate values.
MCHC (Mean Corpuscular Hemoglobin Concentration)

The MCHC is determined according to the HGB and HCT values. The limitations listed for the HGB and HCT will have an effect on the MCHC and may cause inaccurate values.
1-18
Specifications
RDW (Red blood cell Distribution Width)

The red blood cell distribution width is determined according to the RBC count. Nutritional deficiency or blood transfusion - May cause high RDW results due to iron and/or cobalamin and /or folate deficiency.
Plt (Platelets)
Very small erythrocytes (microcytes), erythrocyte fragments (schizocytes) and WBC fragments may interfere with the proper counting of platelets and cause elevated PLT counts. Agglutinated erythrocytes - May trap platelets, causing an erroneously low platelet count. The presence of agglutinated erythrocytes may be detected by observation of abnormal MCH and MCHC values and by careful examination of the stained blood film. Giant platelets in excessive numbers - may cause a low inaccurate platelet count as these large platelets may exceed the upper threshold for the platelet parameter and are not counted. Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of these cells which may cause low PLT counts. Reference (manual) methods may be necessary to obtain an accurate platelet count. Hemolysis - Hemolysed specimens contain red cell stroma which may increase platelet counts. A.C.D. blood - Blood anticoagulated with acid-citrate-dextrose may contain clumped platelet which could decrease the platelet count. Elevated triglycerides and/or cholesterol: may interfere with correct platelet counting. Platelet agglutination - Clumped platelets may cause a decreased platelet count and/or a high WBC count. The specimen should be recollected in sodium citrate anticoagulant to ensure the anticoagulated character depending on agglutination and reanalyzed only for the platelet count. The final PLT result must be corrected for the sodium citrate dilution effect. However, these platelet clumps do trigger flags L1, LL and LL1.
MPV (Mean Platelet Volume)

Giant platelets that exceed the upper threshold of the Platelet parameter may not be counted as platelets. Consequently, these larger platelets will not be included in the instruments calculation of Mean Platelet Volume. Very small erythrocytes (microcytes), erythrocytic fragments (Schizocytes) and white blood cell fragments may interfere with the proper counting and sizing of Platelets. Agglutinated erythrocytes - May trap Platelets, causing an incorrect MPV result. The presence of agglutinated erythrocytes may be detected by observation of abnormal MCH and MCHC values and by careful examination of the stained blood film. Chemotherapy - May also affect the sizing of PLTs.
IMPORTANT
Blood samples collected in EDTA will not maintain a stable Mean Platelet Volume. Platelets collected in EDTA swell depending on the time post-collection and storage temperature.
1-19
Pentra 60 C+
LYM# (Lymphocyte count absolute value), LYM% (Lymphocyte percentage)
The Lymphocyte count is derived from the WBC count. The presence of erythroblasts, certain parasites and erythrocytes that are resistant to lysis may interfere with an accurate LYM count. Limitations listed for the WBC count pertain to the LYM # and % counts as well.
MON# (mononuclear cell count absolute), MON% (Mononuclear percentage)

The mononuclear cell count absolute is derived from the WBC count. The presence of large lymphocytes, atypical lymphocytes, blasts and an excessive number of basophils may interfere with an accurate monocyte count. Limitations listed for the WBC count pertain to the MON # and % counts as well.
NEU# (neutrophil count absolute), NEU% (Neutrophil percentage)

The neutrophils cell count is derived from the WBC cell count. The excessive presence of eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere with an accurate neutrophils count.
EOS# (Eosinophil cell count absolute), EOS% (Eosinophil percentage)

The eosinophil cell count is derived from the WBC cell count. The presence of abnormal granules (degranulated areas, toxic granules...) may interfere with the eosinophil counting.
BAS# (Basophil cell count absolute), BAS% (Basophil percentage)

The Basophil cell count is derived from the WBC cell count.
1-20
Pentra 60 C+
HYDRAULIC&PNEUMATIC PRINCIPLES
1. HYDROPNEUMATIC CONNECTIONS ..................................... 2-2 2. FUNCTION OF VALVES .......................................................... 2-5 3. VALVES LOCATIONS .............................................................. 2-6 4. PNEUMATIC DIAGRAM .......................................................... 2-7
Pentra 60 C+
1. HYDROPNEUMATIC CONNECTIONS
Read this table as follow: LV3_2S means connection to port 2 of valve 3 with a Sleeving.
FROM ABX CLEANER
LENGTH
DIAM.
TO
Abx Cleaner Bottle LV7_3 LV7_2 LV12_1 LV12_2

ABX EOSINOFIX
2.05 2.05 1.52 1.52 1.52 2.05 2.05 1.52 1.52 2.05 2.05 1.52 1.52 1.52 1.52 1.52 1.52 1.02 1.30 1.30 1.02 1.02 1.02 2.05 1.85 0.19 1.52 1.52 1.52 1.52 1.52 1.52 1.52
400 120 230 350 280 400 120 500 120 400 120 500 60 120 350 130 480 205 20 320 130 240 95 420 11 4 20 70 80 335 120 160 120
LV7_1 Diluter Syringe_2 LV12_3 Baso/WBC chamber_1 T3_3 LV8_1 Diluter Syringe_3 Reagent Heater_3S LMNE chamber_3 LV11_1 Diluter Syringe_5 Reagent Heater_10S Reagent Heater_11S BASO/WBC chamber_2 LV6_1 Diluter Syringe_1 DIL1/HGB chamber_2 Sampling Syringe_1 M1_2 photocell LV4_1 T2_2 Injector Syringe_5 LV5_1 T6_2 LMNE flowcell_6 LMNE flowcell_6 E1_1 Anode fitting Isolator_1 E2_1 Ground fitting LV24_2 T4_2 Reagent Heater_5S LMNE chamber_2
Abx Eosinofix Bottle LV8_3 LV8_2 Reagent Heater_4S

ABX BASOLYSE II
Abx Basolyse II Bottle LV11_3 LV11_2 Reagent Heater_9S Reagent Heater_12S

ABX ALPHALYSE
Abx Alphalyse Bottle LV6_3 LV6_2

SAMPLING
Probe_1
LMNE COUNTING
LMNE chamber_4 M1_1 photocell LV4_2 T2_3 Injector Syringe_3 LV5_2 T2_1S LMNE flowcell_Output E1_2 Anode fitting Isolator_2 E2_2 Ground fitting LV24_1 T4_1 Reagent Heater_6S
2-2
Hydraulic & Pneumatic Principles
FROM DILUENT
LENGTH
DIAM.
TO
Diluent Input LV25_1S Diluent Tanker_1 LV3_3S LV3_2S LV2_1S LV1_1S Isolator_2S T1_2 T1_2 T1_3 T1_3 LV1_2 LV2_2 Injector Syringe_1 LMNE flowcell_7 Diluent Tanker_3 LV9_3 LV9_2 LV10_1 T3_1 LV18_1 LV19_1 T12_3 T12_2 Isolator_3 LV32_2 LV19_2 LV18_2 LV10_2 LV21_1 LV22_1 Reagent Heater_8S LV22_2 Reagent Heater_2S LV21_2
2.54 2.54 2.05 2.05 2.05 1.52 1.52 1.52 1.52 1.14 XBA403 1.14 XBA403 1.52 1.02 1.02 1.02 2.05 2.05 2.05 1.52 1.52 1.52 1.52 1.52 1.52 1.30 1.52 1.52 1.52 1.02 1.52 1.52 1.52 1.52 1.52 1.52 1.52
170 140 65 350 120 50 50 200 100 35 35 250 175 205 10 65 480 120 50 15 640 50 190 75 15 215 780 300 115 380 50 200 120 260 120 220
2-3
LV25_2S Diluent Tanker_4S Diluent Tanker_1 LV3_1 Injector Syringe_2 LV2_3S LV1_3S Isolator_1S T1_1 LMNE flowcell_4 LMNE flowcell_4 LMNE flowcell_2 LMNE flowcell_2 T4_3 Injector Syringe_4 LMNE flowcell_5 (Cap) Diluent Tanker_3 LV9_1 Diluter Syringe_4 LV10_3 T3_2 LV18_3 LV19_3 T12_1 Probe Rinsing Body_1 Isolator_1 LV32_1 T5_2 Probe Rinsing Body_2 Sampling Syringe_2 LV21_3 LV22_3 Reagent Heater_7S RBC chamber_1 Reagent Heater_1S DIL1/HGB chamber_1 Baso/WBC chamber_4
Pentra 60 C+
FROM LENGTH WBC/RBC COUNTING
DIAM.
TO
BASO/WBC chamber_3 LV23_1 RBC chamber_2 LV14_1 LV23_2

DRAININGS
1.52 1.52 1.52 1.52 1.52 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.05 2.54 2.54 2.05 2.54 2.54 2.05 1.52 2.05 1.52 1.52 2.05 1.30 2.05 1.52
200 220 430 70 310 30 30 30 70 20 20 70 20 20 60 20 20 55 20 30 20 50 15 280 150 130 125 340 20 140 70 100 400 70 70 185 300 90
2-4
LV23_3 RBC chamber_3 LV14_2 Counting Syringe_2 Counting Syringe_3 Filter_1 LV27_2 T7_3 LV28_2 T7_2 T8_3 LV29_2 T8_2 T9_3 LV30_2 T9_2 T10_2 LV31_2 T10_1 T6_3 T5_3 M2_1 photocell Isolator_1 LV26_1 Draining Syringe_2S T11_3 LV16_1 T11_2 Waste Output LV13_2 Counting Syringe_1 LV15_1 Diluent Tanker_2 Counting Syringe_4 LV17_2 Isolator_2 LV20_2 Draining Syringe_1
Rinse chamber_3 Filter_2 LV27_1 DIL1/HGB chamber_3 LV28_1 T7_1 LMNE chamber_5 LV29_1 T8_1 RBC chamber_4 LV30_1 T9_1 BASO/WBC chamber_5 LV31_1 T10_3 T6_1 T5_1 M2_2 photocell Isolator_2 LV26_3S LV26_2S Counting Syringe_5 LV16_2S T11_1
AIR
Atmosphere LV13_1 Atmosphere LV15_3 LV15_2 Atmosphere LV17_1 Rinse_2 chamber (Atmo.) LV20_1

2. FUNCTION OF VALVES
VALVE # VALVE SUBJECT VALVE FUNCTIONS
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32
DIL LMNE DIL LMNE DIL LMNE LMNE LMNE LYSE CLEANER EOSINOFIX DIL DIL BASOLYSE II CLEANER COUNTING SYRINGE COUNTING SYRINGE TANK COUNTING SYRINGE NEEDLE NEEDLE NEEDLE DRAINING SYRINGE DIL DIL COUNTING SYRINGE LMNE DILUENT TANK FLUSHING SYRINGE CHAMBER GROUP CHAMBER GROUP CHAMBER GROUP CHAMBER GROUP CHAMBER GROUP NEEDLE
Directing optical chamber / LMNE chamber Directing Internal / External sleeving Control Entry / Exit syringe Transfer control LMNE tray /Injection syringe Transfer control LMNE tray /Injection syringe Control Entry / Exit syringe Control Entry / Exit syringe Control Entry / Exit syringe Control Entry / Exit syringe Directing Circuit chamber / Needle circuit Control Entry / Exit syringe Directing WBC/BASO chamber / Needle circuit Set in the atmosphere RBC/PLT count Set in the atmosphere Draining Flushing out of rinsing unit Commutation Diluent interior/exterior Needle Probe cleaning Set in the atmosphere Directing head for WBC count / Valve 22 Directing diluent chamber dil.1 WBC/RBC selection count Exit from the optical chamber Entry of the diluent Flushing of the syringe Drain rinse chamber Drain first dilution chamber Drain LMNE chamber Drain RBC/PLT chamber Drain WBC/BASO chamber Probe cleaning
2-5
Pentra 60 C+
3. VALVES LOCATIONS
Valve blocks are located close to the elements concerned. There are 5 different blocks:
Valves 1 to 11: On the left side, Horizontal block, On the top of the reagent and injection syringes. Valves 12 to 16: On the left side, Vertical block, Beside the counting syringe.
Valves 17 to 19 & 32: On the right side, Horizontal block, On ampling carriage.
Valves 20 to 26: On the right side, Vertical block, Beside the draining syringe. Valves 27 to 31: On the right side, Horizontal block, On top of the blending and count chambers.
2-6

4. PNEUMATIC DIAGRAM
See Pneumatic diagram on next page.
2-7
Pentra 60 C+
ELECTRIC&ELECTRONIC PRINCIPLES
1. MAIN BOARD TEST POINTS ................................................... 3-2 2. MAIN BOARD POTENTIOMETERS ......................................... 3-4 3. PENTRA 60 C+ BOARDS ........................................................ 3.1 Main board XAA 425 C .................................................... 3.2. Optical Preampli board XAA 423 B ................................. 3.3. Motor relay board XAA 427 B ......................................... 3.4. Carriage board XAA 428 C ............................................. 3.5. LED board XAA 429 A .................................................... 3-5 3-5 3-6 3-7 3-8 3-9
4. PENTRA 60 C+ CONNECTORS ............................................. 3-10 4.1. Chamber Heating Connector XBA 386 A ....................... 3-10 4.2. Reagent Heating Coil Connector XBA 387 A .................. 3-11 4.3. Horizontal Carriage Motor XBA 391 A ........................... 3-12 4.4. Upper Fan XBA 393 A ................................................... 3-13 4.5. IR sensor XBA 394 AS ................................................... 3-14 4.6. IR sensor XBA 395 AS ................................................... 3-15 4.7. IR sensor XBA 396 AS ................................................... 3-16 4.8. IR sensor XBA 397 AS ................................................... 3-17 4.9. RBC / WBC coaxe XBA 398 B ...................................... 3-18 4.10. LMNE flowcell coaxe XBA 399 AS ............................... 3-19 4.11. Barcode reader cable XBA 402 AS .............................. 3-20 4.12. Diluent level sensor XDA 605 AS .................................. 3-21 5. PENTRA 60 C+ SYNOPTIC ................................................... 3-22
Pentra 60 C+
1. MAIN BOARD TEST POINTS
TEST POINT DESIGNATION POTENTIOMETER
TARGET
TOLERANCE
REMARK
TP1 HB Blank adjustment TP2 PLT Threshold adjustment TP3 LMNE CIS Threshold adjustment TP4 LMNE OD Threshold adjustment TP5 Draining syringe motor TP6 Counting syringe motor TP7 Dilutor syringe motor TP8 Injection syringe motor TP9 UNUSED TP10 Horizontal carriage motor TP11 Sample syringe motor TP12 Probe carriage motor TP13 RBC Threshold adjustment TP14 BASO Threshold adjustment TP15 BASO Comparator TP16 RBC Comparator TP17 PLT Comparator TP18 LMNE CIS Comparator TP19 LMNE OD Comparator TP20 RBC line height adjsutment TP21 BASO gain adjustment TP22 PLT line height adjustment TP23 LMNE Resistive gain adjustment TP24 LMNE Optical gain adjustment TP25 RBC line width adjustment TP26 RBC line reject adjustment TP27 RBC line pulse adjustment TP28 BASO line width adjustment TP29 BASO line reject adjustment TP30 BASO line pulse adjustment TP31 PLT line width adjustment TP32 PLT line reject adjustment TP33 PLT line pulse adjustment TP34 LMNE Resistive line width adjustment TP35 LMNE Resistive line reject adjustment TP36 LMNE Resistive line pulse adjustment TP37 LMNE Optical line width adjustment TP38 LMNE Optical line reject adjustment TP39 LMNE Optical line pulse adjustment
R248 R159 R160 R161 R149 R150 R151 R152 R154 R155 R156 R158 R157
4.7V 300mV 650mV 350mV 4.8V 4.8V 4V 3V 3V 2V 4.5V 300mV 300mV
+/- 0.05V +/- 5mV +/- 5mV +/- 5mV +/- 0.05V +/- 0.05V +/- 0.05V +/- 0.05V +/- 0.05V +/- 0.05V +/- 0.05V +/- 5mV +/- 5mV
R133 R134 R135 R136 R148 R142
XX V XX V XX V XX V XX V 10s
+/- 50mV +/- 50mV +/- 50mV +/- 50mV +/- 50mV +/- 0.5s
Factory Adjusted Factory Adjusted Factory Adjusted Factory Adjusted Factory Adjusted Factory Adjusted
R139
10s
+/- 0.5s
Factory Adjusted
R144
10s
+/- 0.5s
Factory Adjusted
R145
15s
+/- 0.5s
Factory Adjusted
R147
5s
+/- 0.5s
Factory Adjusted
3-2
Electric & Electronic Principles

TEST POINT DESIGNATION POTENTIOMETER
TARGET
TOLERANCE
REMARK
TP40 5V Power supply TP41 12V Power supply TP42 24V Power supply TP43 -12V Power supply TP44 GROUND TP45 GROUND TP46 GROUND TP47 GROUND TP48 LMNE Draining sensor
5V 12V 24V -12V
R286
<1V
With water
TP49 UNUSED TP50 Reagent heating system CTN voltage TP51 Thermostated room heating sytem CTN voltage TP52 Draining sensor TP53 LMNE Lamp TP54 GROUND TP55 TP56 TP57 GROUND GROUND GROUND R287 R11 <1V 6V With water
TP58 GROUND TP59 GROUND TP60 LMNE Resistive line reject2 adjustment TP61 LMNE Resistive line reject2 adjustment
R409 R410
50s 250s
+/- 2s +/- 5s
Factory Adjusted Factory Adjusted
3-3
Pentra 60 C+
2. MAIN BOARD POTENTIOMETERS
POTENTIOMETER DESIGNATION
TEST
POINT
TARGET
TOLERANCE
REMARK
R133 RBC line height adjsutment R134 BASO gain adjustment R135 PLT line height adjustment R136 LMNE Resistive gain adjustment R139 BASO line reject adjustment R142 RBC line reject adjustment R144 PLT line reject adjustment R145 LMNE Resistive line reject adjustment R147 LMNE Optical line reject adjustment R148 LMNE Optical gain adjustment R149 R150 R151 R152 Draining syringe motor Counting syringe motor Dilutor syringe motor Injection syringe motor
TP20 TP21 TP22 TP23 TP29 TP26 TP32 TP35 TP38 TP24 TP5 TP6 TP7 TP8 TP10 TP11 TP12 TP14 TP13 TP2 TP3 TP4 TP1 4.8V 4.8V 4V 3V 3V 2V 4.5V 300mV 300mV 300mV 650mV 350mV 4.7mV +/- 0.05V +/- 0.05V +/- 0.05V +/- 0.05V +/- 0.05V +/- 0.05V +/- 0.05V +/- 5mV +/- 5mV +/- 5mV +/- 5mV +/- 5mV +/- 0.1mV
F.A. F.A. F.A. F.A. F.A. F.A. F.A. F.A. F.A. F.A.
R154 Horizontal carriage motor R155 Sample syringe motor R156 Needle carriage motor R157 BASO Threshold adjustment R158 RBC Threshold adjustment R159 PLT Threshold adjustment R160 LMNE CIS Threshold adjustment R161 LMNE OD Threshold adjustment R248 HB Gain adjustment
3-4

3. PENTRA 60 C+ BOARDS
3.1 Main board XAA 425 C
3-5
Pentra 60 C+
3.2. Optical Preampli board XAA 423 B
OPTICAL PRE-AMPLI BOARD: Perform an absorbance measurement This is an Intensity / Tension convertissor A photodiode measures absorbance of light through the cell, the optical pre-ampli board returns an intensity proportionnal to the light signal (Light signal is proportionnal to the cell size)
3-6

3.3. Motor relay board XAA 427 B
MOTOR RELAY BOARD: Interconnection board where all the different motors and end of run sensor are connected This board is connected to the main board with a flat cable
3-7
Pentra 60 C+
3.4. Carriage board XAA 428 C
CARRIAGE BOARD: Interconnection board where all the carriage electrical functions are connected This board is connected to the main board with a flat cable
3-8

3.5. LED board XAA 429 A
LED BOARD: This board supports LEDs on the front panel. They give instrument state: Red: instrument is busy Green: instrument is ready to sample
3-9
Pentra 60 C+
4. PENTRA 60 C+ CONNECTORS
4.1. Chamber Heating Connector XBA 386 A
3-10

4.2. Reagent Heating Coil Connector XBA 387 A
3-11
Pentra 60 C+
4.3. Horizontal Carriage Motor XBA 391 A
3-12

4.4. Upper Fan XBA 393 A
3-13
Pentra 60 C+
4.5. IR sensor XBA 394 AS
3-14

3-15
Pentra 60 C+
3-16

3-17
Pentra 60 C+
4.9. RBC / WBC coaxe XBA 398 B
3-18

4.10. LMNE flowcell coaxe XBA 399 AS
3-19
Pentra 60 C+
4.11. Barcode reader cable XBA 402 AS
3-20

4.12. Diluent level sensor XDA 605 AS
3-21
Pentra 60 C+
5. PENTRA 60 C+ SYNOPTIC
3-22
jhfh
Pentra 60 C+
ANALYSIS CYCLE TECHNOLOGY
1. ANALYSIS CYCLE DESCRIPTION ............................................ 2-2 2. MEASURING PRINCIPLES ...................................................... 2-4 2.1. Multi Distribution Sampling System (MDSS) .................... 2-4 2.2. RBC / Plt detection principles ......................................... 2-5 2.3. Hgb measurement ......................................................... 2-6 2.4. Hct measurement .......................................................... 2-7 2.5. RDW calculation ............................................................. 2-7 2.6. MCV, MCH, MCHC calculation ....................................... 2-7 2.7. MPV Measurement ........................................................ 2-8 2.8. Pct calculation ................................................................ 2-8 2.9. PDW calculation ............................................................. 2-8 2.10. WBC and differential count ........................................... 2-9 2.10.1. General principles .................................................. 2-9 2.10.2. BASO / WBC Count .............................................. 2-9 2.10.3. LMNE matrix ........................................................ 2-10
Pentra 60 C+
1. ANALYSIS CYCLE DESCRIPTION
Aspiration of blood sample from the tube trough the cap (53l) Translation of sampling carriage over Rinse chamber.
Cleaning of the needles: Internal cleaning: 1ml inside piercing needle External cleaning: 2ml outside piercing needle Internal cleaning: 1ml inside piercing needle and reject of 3l of blood (not used).
Translation of sampling carriage DIL1 chamber (first dilution), descent of

the needle.
Positioning the needle point into a tangential flux of ABX diluent (1.65ml)
and synchronised distribution of 10l of blood.
Translation of sampling carriage over WBC/BASO count chamber. Positioning the needle point into a tangential flux of 2ml of ABX BASOLYSE
II and synchronised distribution of 10l of blood.
Dilution Blood Volume BASOLYSE II Volume Dilution Rate
10 l 2000 l 1/200
Translation of sampling carriage over LMNE mixing chamber. Positioning the needle point into a tangential flux of 1ml of ABX Eosinofix
and synchronised distribution of 25l of blood.
Dilution Blood Volume Eosinofix Volume Diluent Volume Final Dilution Rate
4-2
25 l 1000 l 1000 l 1/80
Analysis Cycle Technology

Translation of sampling carriage over Rinse chamber Rinsing of the needles: Internal rinsing: 0.8ml inside sampling needle External rinsing: 0.5ml outside sampling needle Internal rinsing: 0.5ml inside sampling needle External rinsing: 0.3ml outside sampling needle
WBC and LMNE counting: 1ml of diluent into LMNE chamber Dilution transfer trough LMNE flowcell and counting during 12 seconds WBC\BASO counting
Translation of sampling carriage over DIL1 chamber Sample of the 42.5l of the first dilution.
Translation of sampling carriage over Rinse chamber. Rinsing of the exterior of sampling needle with 0.4ml of diluent
Translation of sampling carriage over RBC and PLT count chamber. Distribution of the 42.5l of first dilution into a flux of 2.20ml of diluent. Distribution of 0.3ml of diluent from the interior of the needle.
Dilution Initial Blood Volume Reagent Volume (RBC chamber) Final Dilution Rate
10 l 2500 l 1/10000
Hgb: Distribution of 0.36ml of ABX ALPHALYSE into DIL1 chamber while bubbling. WBC\BASO chamber rinsing: Draining and filling with 1ml of ABX CLEANER and 1.5ml of diluent RBC\Plt counting and Hgb measurement Chambers cleaning and Hgb blank Draining and filling of DIL1 chamber with 2.7ml of diluent Measurement of Hgb blank Flowcell rinsing (about 1.6ml of diluent) Draining and filling of RBC chamber with 2.5ml of diluent
4-3
Pentra 60 C+
2. MEASURING PRINCIPLES
2.1. Multi Distribution Sampling System (MDSS)

+ ABX PENTRA 60 C+ analysis
cycle needs 3 blood specimens distributed as follows: one specimen for the first RBC/Plt dilution and the Hgb measurement. another specimen for the BASO/WBC count. the last specimen for the LMNE matrix. These 3 specimens are provided by means of a multidistribution principle: 53 l of blood are aspirated inside the needle (sufficient volume for all dilutions) then divided up and distributed to the chambers with reagents. Extremities of the specimen are not delivered to the dilutions.
Diluent Air bubble Not used LMNE Dilution WBC\BASO Dilution RBC\PLT\HGB first dilution Not used
SPECIMENS INSIDE THE NEEDLE
+ Specimen distribution in
the chambers is carried out in a tangential flow of reagent which allows perfect mixing of the dilution and avoids any viscosity problems (this multi distribution in a reagent flow is an ABX DIAGNOSTICS patent). Needle
Reagent input
Tangential Flow Mix Mixing chamber
BLOOD DISTRIBUTION IN A TANGENTIAL FLOW
4-4

2.2. RBC / Plt detection principles
+ Measurement of
impedance variation generated by the passage of cells through a calibrated micro aperture. The specimen is diluted in an electrolytic diluent (current conductor) and pulled through the calibrated micro-aperture. Two electrodes are placed on either side of the aperture. Electric current passes through the electrodes continuously. When the cell passes through the aperture, electric resistance between the two electrodes increases proportionately with the cell volume. The generated impulses have a very low voltage, which the amplification circuit increases, so that the electronic system can analyze them and eliminate the background noise. Pulse Time Solution to be ananlyzed
Vacuum = Constant
Volts Electrodes
Analyzing electronic circuit
TECHNICAL
COUNTS
CHARACTERISTICS OF THE
RED BLOOD CELL
AND
PLATELET
Initial blood volume Vol. ABX DILUENT Final dilution rate** Temperature of reaction
10 l 2500 l 1/10000 35C
Method Aperture diameter Count vacuum Count period

OUT
Impedance 50 m 200 mb 2 X 5 seconds
**: TWO SUCCESSIVE DILUTIONS ARE CARRIED PRIMARY DILUTION FOR RBC AND PLT:
Blood (l): 10 ABX DILUENT (L): Dilution (l): 42.5 ABX DILUENT(L):
FINAL
DILUTION:
1700
AND
dilution:
PLT (FROM
1/170
SECONDARY DILUTION RBC
THE PRIMARY DILUTION):
2500
dilution:
1/58.8
1/170 X 1/58.8 =1/10000
4-5
Pentra 60 C+
Results RBC
Number of cells counted per volume unit x Calibration coefficient.
Histograms
RBC: Distribution curves on 256 counting channels from 30fl to 300fl. Plt: Distribution curves on 256 channels from 2fl to a mobile threshold. This threshold moves according to the microcyte population present in the analysis area.
2.3. Hgb measurement

The hemoglobin released by the lysis of the red blood cells combines with the potassium cyanide to form the chromogenous cyanmethemoglobin compound. This compound is then measured through the optical part of the first dilution chamber using a spectrophotometric technique at a wavelength of 550 nm.
TECHNICAL
CHARACTERISTICS FOR THE MEASUREMENT OF THE
HEMOGLOBIN:
Volume of blood Volume ABX DILUENT Volume ABX ALPHALYSE Complement ABX DILUENT Final dilution rate Temperature of reaction
10 l 1700 l 400 l 400 l 1/250 35C
Method Wavelength
Photometry 550 nm
Result
Final Hgb result represents: Absorbance value obtained x coefficient of calibration.
4-6
2.4. Hct measurement

The height of the impulse generated by the passage of a cell through the microaperture is directly proportional to the volume of the analyzed RBC. The hematocrit is measured as a function of the numeric integration of the MCV.
2.5. RDW calculation

The study of the RBC distribution detects erythrocyte anomalies linked to anisocytosis. A Red Cell Distribution Width (RDW) will enable you to follow the evolution of the width of the curve in relation to the cell number and average volume. K SD RDW= MCV With: K = system constant SD = Determined standard deviation according to statistical studies on cell distribution. MCV = Mean Corpuscular Volume of erythrocytes
2.6. MCV, MCH, MCHC calculation

MCV (Mean Cell Volume) is calculated directly from the RBC histogram. MCH (Mean Cell Hemoglobin) is calculated from the Hgb value and the RBC number. The mean hemoglobin weight in each RBC is given by the formula: Hgb MCH (pg) = RBC MCHC (Mean Corpuscular Hemoglobin Contained) is calculated according to the Hgb and Hct values. Mean Hgb concentration in the total volume of RBC is given by the formula: Hgb MCHC (g/dL) = Hct x 100 x 10
4-7
Pentra 60 C+
2.7. MPV Measurement

The MPV (Mean Platelet Volume) is directly derived from the analysis of the platelet distribution curve.
2.8. Pct calculation

Thrombocrit is calculated according to the formula: Plt (103/l) x MPV (m3) Pct% = 10 000
2.9. PDW calculation

PDW (Platelet Distribution Width) is calculated from the Plt histogram. The PDW is represented by the width of the curve between 15% of the number of platelets starting from 2 fl (S1), and 15% of the number of platelets beginning with the variable top threshold (S2).
S1 PDW
S2
4-8

2.10. WBC and differential count
2.10.1. General principles
The WBC count is carried out twice by two different sensors: In the BASO count chamber at the same time as the BASOS count, In the optical chamber during the acquisition of the LMNE matrix. The reference count is the one obtained in the WBC and BASO count chamber.
2.10.2. BASO / WBC Count

Detection principle is the same as for RBC. Differentiation betwen BASOs and other leukocytes is obtained by means of the BASOLYSE II specific lysing action. All the WBCs are counted between the electrical threshold <0> threshold <BA3>. The basophils are located from threshold <BA2> to threshold <BA3>.
BA1
BA2
BA3
WBC
BASO
TECHNICAL CHARACTERISTICS
OF THE
WBC/BASO
COUNT
Blood volume BASOLYSE II volume Dilution rate Reaction temperature
10 l 2000 l 1/200 35 C
Method Ruby diameter Depression of count Duration of the count
Impedance 80 m 200 mb 2 X 6 seconds
Results
WBC: Number of cells per volume x coefficient of calibration. BASO: Number of cells per volume x coefficient of calibration in percentage regarding the total number of leukocytes (BASO + WBC nuclei)
4-9
Pentra 60 C+
2.10.3. LMNE matrix
The WBC and Differential count are based on 3 essential principles:
2) SECOND FOCUSED FLOW FOR OPTICAL DETECTION
1- The double hydrodynamic sleeving

DHSS (ABX DIAGNOSTICS patent)
2- The volume measurement

impedance changes.
3- The measurement of transmitted light

with 0 angle, which permits a response according to the internal structure of each element and its absorbance by means of incident light diffusion. 25l of whole blood is delivered to the LMNE chamber in a flow of EOSINOFIX. This reagent lyses the RBC, stabilizes the WBC in their native forms and stains the eosinophil nuclei with a specific coloration. The solution is then stabilized with diluent and transferred to the measuring chamber. Each cell is measured both in absorbance (cytochemistry) and resistivity (volume).
1) PRIMARY FOCUSED FLOW FOR IMPEDANCE MEASUREMENT

TECHNICAL
THE MATRIX CHARACTERISTICS OF THE
WBC
COUNT DURING THE ACQUISITION OF
Blood volume Eosinofix volume Diluent volume Final dilution rate Reaction temperature Incubation duration
25 l 1000 l 1000 l 1/80 35 C 12 s.
Method Ruby diameter Flow diameter Injection duration Volume injected
Impedance with hydrofocus 60 m 42 m 12 seconds 72 l
Results
From these measurements, a matrix is drawn up with volumes on the X-axis and optical transmission on the Y-axis. The study of the matrix image permits the clear differentiation of 4 out of 5 leukocyte populations. As a matter of fact, the basophil population is very small compared to the other 5 in a small blood sample.
4-10
NEUTROPHILS
EOSINOPHILS
ABSORBANCE
LMNE
LIC
VOLUME
LYMPHOCYTES
ALY
MONOCYTES
4-11
Pentra 60 C+
MONOCYTES: The monocytes, being cells with large kidney shaped nuclei and a large non-granular cytoplasm, will neither be scattered nor absorb a large amount of light. They will therefore be positioned in the lower part of the optical axis but clearly to the right of the volume axis. Certain large monocytes can be found on the right side of the matrix in the lower LIC (Large Immature Cells) zone. The immature granulocytic cells are detected by their larger volumes and by the presence of granules which increase the intensity of the scattered light. Therefore, cells such as metamyelocytes will be found clearly to the right of the neutrophils and nearly at the same level. Myelocytes and promyelocytes will be found in saturation position on the far right of the matrix. These last three populations will be counted as LIC (Large Immature Cells) and their given results are included in the neutrophil value. The blast cells will be found generally to the right of the monocytes, and, as such, will increase the LIC count. Small blasts will be found between the normal lymphocytes and monocytes. Platelets and debris from erythrocyte lysis represent the background noise population located in the lower left area of the matrix. Most of the population partition thresholds are fixed and give the limits of the morphological normality of leukocytes. Changes in the morphology of a population will be expressed on the matrix by a shifting of the corresponding population.
LYMPHOCYTES: The lymphocytes being small with regular shape, are positioned in the lower part of both the optical axis and volume axis. Normal lymphocyte populations are generally observed with a good volume homogeneity. Large lymphocytes are detected in the ALY (Atypical Lymphocytes) zone, where reactive lymphoid forms, stimulated lymphocytes and plasmocytes are also to be found. The far left side of the lymphocyte zone should normally be empty, but when small lymphocytes are present, population may exist in this area. The presence of platelet aggregates is detected by a distribution pattern that moves from the origin of the matrix (background zone) into the lymphocyte zone. The NRBCs with their cytoplasmic membranes lysed like the erythrocytes, will have their nuclei situated to the far left side of the lymphocyte zone.
EOSINOPHILS: With reagent action on cytoplasmic membranes, the leukocytes keep their native size and only eosinophils are colored for optical separation. Eosinophils will be situated in the upper part of the optical Y-axis due to their strong absorbance qualities and their size, which is nearly equivalent to large neutrophils.
NEUTROPHILS: The neutrophils, with their cytoplasmic granules and their generally segmented nuclei, will scatter light depending on their morphological complexity. A hypersegmented neutrophil will give an increased optical response with respect to a young neutrophil population which will be in the upper position of the optical axis depending on the presence of segmentation and/or granules.
4-12
Pentra 60 C+
SOFTWARE VERSIONS
1. SERVICE SOFTWARE OVERVIEW ........................................... 5-2 2. SOFTWARE RELEASES .......................................................... 5-3 2.1. Software releases table .................................................. 5-3 2.2. Software modifications ................................................... 5-3
Pentra 60 C+
1. SERVICE SOFTWARE OVERVIEW
MECHANICAL SYSTEMS
+ Initialization + Check motors + Check valves + Sampling needle adjustment + Maintenance carriage position + Park syringe position
HYDRAULIC SYSTEMS
+ Drain chamber + Rinse + Cleaning cycle + Prime cycles
TECHNICIAN ADJUSTMENT
+ Sampling needle adjustment + Draining sensor adjustment + Transfer sensor adjustment + Vacuum adjustment + Cycles + Heating coil adjustment + Thermostatic compartment adjustment + Bublbing + Carriage dilution positions
TECHNICIAN MEASUREMENT
+ Gain adjustment + LMNE calibration + Pulse adjustment

BURN IN CYCLE
+ Burn in parameters
RS232 TO P60
+ Connection test & command + Service mode + Configure Analyzer Main board
5-2
Software Versions
2. SOFTWARE RELEASES
2.1. Software releases table

TECHNICAL NOTE
RAH801A RAN015A
SOFTWARE REVISION
V1.61 V2.2.9
DATE
14/04/2001 15/02/2004
2.2. Software modifications

Between software released, the software or techchnical evolutions may be described in Technical notes.
5-3
Pentra 60 C+
OUTPUT FORMAT
Refer to document ABX Diagnostics OUTPUT FORMAT RAA024
Pentra 60 C+
ALARMS & ERROR LIST
1. TRANSMISSION ...................................................................... 7-2 2. DATABASE ............................................................................. 7-2 3. WORKLIST ............................................................................. 7-2 4. CALIBRATION ........................................................................ 7-3 5. TECHNICAL OPERATIONS ..................................................... 7-3 6. PRINTER ................................................................................ 7-3
Pentra 60 C+
1. TRANSMISSION
DISPLAYED
MESSAGE
CAUSES
ACTION
No ENQ Character Received On RS232 No ACK Character Received On RS232 Write Error On RS232 Internal Error On RS232 Time Out Reached Too Many Characters Received On RS232 Connection To Analyser Failed Defect on transmission operations Check RS232 setup Menu\Service\RS232 TO P60
Time out
Disconnect/Reset/Connect from menu RS232 TO P60
Communication to S.I.L. : Spooler full, result lost ! Result transmission capacity exceeded, only the first XX will be sent Connection Failure - - - - - -
2. DATABASE
DISPLAYED
MESSAGE
Similar Reference Found In The Database Result Not Recorded, File Full Result Not Recorded, Write Error In Database Insufficient free disk space ! There Is No Valid Backup Abnormal Backup Termination ! Abnormal Restore Termination ! File XX Not Found In YY Directory
3. WORKLIST
DISPLAYED
MESSAGE
Sample Id Already Exists In Worklist ! Rejected Result Not Recorded Invalid Date Invalid Gender Entry Patient Name Field Cannot Be Empty ! Sample Id Field Cannot Be Empty ! Patient Information Cannot Be Modified, Result Already Exist !
7-2
Alarms & Error list

4. CALIBRATION
DISPLAYED
MESSAGE
Minimum Of 3 Results Required For Calibration Calibration Failed : Confirm To Force Calibration Calibration Failed : Coef. Out Of Range Minimum Of 5 Results Required To Calibrate
5. TECHNICAL OPERATIONS
DISPLAYED
MESSAGE
Values Out Of Range : [XX] YY Value Out Of Range [XX - ZZ] YY Value Cannot Be Less Than XX Value YY Value Cannot Be Less Than 1 YY Value Must Be Positive Access denied Invalid Value(s) On XX Pathological Limits Thresholds not saved. - - - - Acceptance conditions violated:
6. PRINTER
DISPLAYED
MESSAGE
Are windows messages
7-3
Pentra 60 C+
MAINTENANCE
1. MAINTENANCE ...................................................................... 1.1. Introduction ..................................................................... 1.2. Daily customer maintenance .......................................... 1.3. Weekly customer maintenance ...................................... 1.4. Maintenance table .......................................................... 2. MAINTENANCE KITS ............................................................. 2.1. 6 month maintenance kit ................................................ 2.2. Yearly maintenance kit ................................................... 2.3. Pistons maintenance kit ................................................. 2.4. Fitting kit ......................................................................... 2.5. Screws kit ...................................................................... 3. PROCEDURES ....................................................................... 3.1. Required tools and product ............................................ 3.2. Procedure chart table .................................................... 3.3. Procedure chart table in maintenance order .................. 8-2 8-2 8-2 8-2 8-3 8-4 8-4 8-4 8-5 8-5 8-6 8-7 8-7 8-8 8-9
Pentra 60 C+
1. MAINTENANCE
1.1. Introduction
WARNING
Customer maintenance has to be carried out according to the recommended frequency chart table and after having attended an ABX approved customer training course. The system warranty may be affected if damage occurs after a non trained technician intervenes or if replaced spare parts and consumables do not come from an ABX approved origin.
1.2. Daily customer maintenance

No special adjustments or maintenance has to be done on your equipment if the recommended startup and shutdown procedures are explicitly respected. See the ABX PENTRA 60 C+ User Manual for the daily rinsing and cleaning of the system.
1.3. Weekly customer maintenance

An overall check for cleanliness of the system is recommended every week. All traces of blood or reagent have to be wiped off as soon as possible using a piece of cloth and distilled water.
CAUTION
Never use solvent or abrasive cleaning material to clean the system.
8-2
Maintenance
1.4. Maintenance table
The ABX PENTRA 60 C+ maintenance is scheduled on 3 different maintenance kits. The maintenance schedule depends on the number of cycles per day, follow the chart table to know the kit part number to use:
CYCLES PER DAY -
<30 2Y X X X X X X X X X X X X X
30 to 120 6M 1Y X X X X X X X X X X X X X
18M
>120 2Y X X X X X X X X X X X X X 6M X X X X X X X X X X 1Y X
MAINTENANCE EACH - 1Y Sampling probe Oring Reagent syringe Oring Draining and Counting syringes Oring Sampling syringe 5DIFF syringe Draining chamber Optical bench lamp LMNE flowcell coaxial Diluent reservoir joint Counting heads Sampling probe replacement Reagent pistons replacement Counting and Draining syringe pistons replacement X X X X X X X X X X
PART NUMBER KIT TO USE
X X X
XEA710BS X X X X X XEA486BS X X X X X XEA630CS X X Procedure: RAS309 Procedure: RAS308 Procedure: RAS307
8-3
Pentra 60 C+
2. MAINTENANCE KITS
2.1. 6 month maintenance kit

KIT XEA710BS P/N DESIGNATION QTY
FAA013A FAA055A FAA057A FAA065A XDA621A XDA622A XEA019A
Oring 1.80x1.20 Nitrile (Rinsing block) Oring 1.07x1.27 Fluorocarbon (Rinsing block) Oring 5x1 Nitrile (Rinsing block) Oring 6.30x2.40 Silicon (Reagent syringe) Oring 30.80x3.60 + Washer (Vacuum/Waste pump) Oring 15.54x2.62 + Washer (Reagent syringe) Silicon grease
1 1 1 1 2 4 1
2.2. Yearly maintenance kit

KIT XEA486BS P/N DESIGNATION QTY
DAJ007A FAA040A FAA046A FAA064A FAA066A FAA067A GBG275A JAJ007A XEA286AS XEA663A JAJ007A
9.5V 20W lamp Oring 12.10x2.70 Silicon Oring 2.75x1.60 Viton Oring 1.42x1.52 Fluorocarbon Oring 13.10x1.60 Silicon Oring 2.40x1.90 Fluorocarbon Flat EPO joint 0.5 Polyethylene ribbon Waste chamber Joint + Washer Coaxial kit LMNE flowcell Polyethylene tape
1 1 2 2 4 4 4 1m 1 1 1m
8-4
Maintenance
2.3. Pistons maintenance kit

KIT XEA630CS P/N DESIGNATION QTY
GBC030A GBC031A GBG169A GBG260A GBG274A GBG288A KAA009A
Reagent syringe piston Reagent syringe piston Sampling carriage percutor 31mm piston spacer 31mm piston Sampling carriage CHC M3 screw
4 1 1 2 2 1 6
2.4. Fitting kit

The XEA311 kit contains the most common pneumatic and hydraulic parts.
KIT XEA311 P/N DESIGNATION
QTY
EAA005A EAA006A EAA009A EAB002A EAB003A EAB005A EAB006A EAB009A EAB010A EAA013A EAA014A EAB026A EAB021A EAB035A EAB014A
Straight connector 1.6mm 1/8 Straight connector Bent connector I=1.5 10/32 L Connector Connector 4mm H19.5 T Connector 1.6mm T10-6 T Connector 2.3mm Straight connector 1.6mm Straight connector 2.3mm Bent connector 1/8 Straight connector 1/8 Y Connector 2.5mm Y Connector 3mm T Connector 2.3mm T220-6 Straight connector Y 1.5mm/1.5mm
10 10 10 10 20 30 30 30 30 10 10 3 3 10 10
8-5
Pentra 60 C+
2.5. Screws kit
The XEA293 kit contains the most common screws used in ABX PENTRA 60 C+:
KIT XEA293 AS P/N DESIGNATION
QTY
KAA002A KAA003A KAA005A KAA006A KAA009A KAA011A KAA015A KAA016A KAA017A KAA021A KAA028A KAB002A KAB003A KAB004A KAB005A KAC002A KAE003A KAH001A KAH002A KAH003A KAH024A KAJ001A KAJ002A KAJ003A KAJ007A KAJ008A KAJ009A KAJ010A KAM002A KAM003A KAM004A KAM005A KAM006A KAM010A KAM011A KAM013A KAM017A KAB016A
CHC M3x6 Screw CHC M3x8 Screw CHC M3x12 Screw CHC M3x14 Screw CHC M3x20 Screw CHC M3x30 Screw CHC M4x8 Screw CHC M4x12 Screw CHC M4x16 Screw CHC M5x10 Screw CHC M4x20 Screw FHC M3x6 Screw FHC M3x8 Screw FHC M3x10 Screw FHC M3x12 Screw TC M3x5 Screw BHC M4x12 Screw HU M3 Nut HU M4 Nut HU M5 Nut Nut 1/4-28 (US Std) M Washer Diam.3 M Washer Diam.4 M Washer Diam.5 AZ Washer Diam.3 AZ Washer Diam.4 AZ Washer Diam.5 W Grower Washer Diam.5 Plastic Crosspiece M3x6 Lg.8 Plastic Crosspiece M4x6 Lg.8 Plastic Crosspiece M3.5x6 Lg.6 Plastic Crosspiece M3.5x6 Lg.8 Plastic Crosspiece M3.5x6 Lg.12 Plastic Crosspiece M3x6 Lg.10 Plastic Crosspiece M3.5x6 Lg.5.7 Plastic Crosspiece M3.5x6 Lg.10 Brass Crosspiece M3.5x6 Lg.3 FX M3x8 Screw
20 20 20 20 20 20 20 20 20 20 20 20 5 20 20 20 20 20 20 20 10 20 20 20 20 20 20 20 10 10 10 10 10 10 10 10 10 5
8-6
Maintenance
3. PROCEDURES
NOTE
Maintenance and adjustments required for ABX PENTRA 60 C+ are divided into procedures according to concerned assemblies. This should make any update easier as all interventions can be done with the corresponding procedure on its own. Each procedure has to be read entirely before starting intervention: RED Procedure concerns an Adjustment BLEU Procedure concerns Maintenance GREEN Procedure concerns one Dismantling
WARNING
Procedures must be performed on a clean instrument. If the instrument is suspected not to be perfectly clean, perform a Concentrated cleaning. Disposable gloves should be worn.
3.1. Required tools and product

TOOLS / PRODUCTS DESIGNATION P/N
HEXAGONAL KEYS EMPTY SAMPLE TUBES DYNAMOMETRIC SCREW DRIVER A302 SILICONE GREASE DYNAMOMETRIC SCREW DRIVER A301 GREASE FOR MECHANICAL ASSEMBLIES DYNAMOMETRIC SCREW DRIVER A300 SOFT TISSUE CLAMPS LIQUID SOAP SCALPEL DISTILLED WATER CUTTING PLIERS MICROPIPETTE TIP PAIR OF SCISORS FLAT PIECE OF STIFF PLASTIC VOLTMETER SYRINGE 5ML FLAT SCREWDRIVER LATEX RBC BARFLEX FELT PEN THERMOMETER TORX KEYS
MAG019A LAM004A MAG020A XEA381AS MAG013
LAD002AS
8-7
Pentra 60 C+
3.2. Procedure chart table
P/N PROCEDURE CONCERNS
RAS290 RAS297 RAS298 RAS299 RAS300 RAS303 RAS305
RAS306 RAS307 RAS308
RAS309 RAS310 RAS311 RAS312 RAS313
RAS314 RAS315 RAS316 RAS317 RAS318 RAS319 RAS320
Probe Home position Check and Adjustment - Probe position Check and Adjustment CHANGEMENT OF KEYBOARD TYPE INSTALLATION Unpacking - Working conditions - General description - Printer & instrument connection - Reagent connections - Priming & Startup CHECK UP AFTER Instrument check up: Repeatability - Calibration INTERVENTION TUBE HOLDER ADJUSTMENT LMNE BALANCE ADJUSTMENT TEMPERATURE ADJUSTMENT Reagent heating system temperature Check - Reagent heating system temperature Adjustment - Thermostatedcompartment temperature Check - Thermostated compartment temperature Adjustment FRONT PANEL DISMANTLING Front panel Dismantling - Front panel Mounting 6 MONTH MAINTENANCE Sampling Probe: O'ring Replacement - Reagents syringe: O'rings Replacement - Vacuum/Waste pump: O'rings Replacement YEARLY MAINTENANCE Sampling syringe: O'ring Replacement - 5DIFF syringe: O'ring Replacement - Draining chamber: O'ring Replacement - Optical Bench: Lamp & LMNE flowcell coaxial Replacment - Diluent reservoir: Joint and washer Replacement - Counting Heads: RBC/WBC Coaxial, O'ring and Aperture joint Replacement EVERY 2 YEARS Sampling probe: Probe & Guide Replacement - Reagents syringe: MAINTENANCE KIT Pistons Replacement - Vacuum/Waste pump: Piston Replacement DECONTAMINATION & RINSE Instrument Decontamination & Rinse LMNE FLOWCELL Optical Bench Check and Adjustment ADJUSTMENT CHAMBERS ADJUSTMENT Alignment Check and Adjustment of chambers assembly MAIN BOARD ADJUSTMENT HGB Blank Adjustment - RBC/PLT Gain Adjustment - WBC/BASO Gain Adjustment - Drain sensor Adjustment - LMNE sensor Adjustment Motor Current Adjustment - Thresholds Adjustment VACUUM ADJUSTMENT Draining syringe vacuum Check - Counting syringe vacuum Check Counting syringe vacuum Adjustment BUBBLING ADJUSTMENT Bubbling Adjustment Menu presentation STEP BY STEP ANALYSIS CYCLE OPTICAL BENCH DISMANTLING Optical bench Dismantling - Optical bench Replacement - Emission & REPLACEMENT gun location Control - Optical bench lamp alignement Control BARCODE READER Instrument configuration for Barcode reader CONFIGURATION POWER SUPPLY REPLACEMENT HEATER ASSY REPLACEMENT
PROBE ADJUSTMENT
8-8
Maintenance
3.3. Procedure chart table in maintenance order
P/N PROCEDURE CONCERNS
RAS298 RAS307 RAS308
RAS309 RAS310 RAS312 RAS290 RAS313
RAS305
RAS314 RAS315 RAS311 RAS303 RAS300 RAS319 RAS299 RAS317 RAS306 RAS318 RAS320 RAS301 RAS297 RAS316
Unpacking - Working conditions - General description - Printer & instrument connection - Reagent connections - Priming & Startup 6 MONTH MAINTENANCE Sampling Probe: O'ring Replacement - Reagents syringe: O'rings Replacement - Vacuum/Waste pump: O'rings Replacement YEARLY MAINTENANCE Sampling syringe: O'ring Replacement - 5DIFF syringe: O'ring Replacement - Draining chamber: O'ring Replacement - Optical Bench: Lamp & LMNE flowcell coaxial Replacment - Diluent reservoir: Joint and washer Replacement - Counting Heads: RBC/WBC Coaxial, O'ring and Aperture joint Replacement EVERY 2 YEARS Sampling probe: Probe & Guide Replacement - Reagents syringe: MAINTENANCE KIT Pistons Replacement - Vacuum/Waste pump: Piston Replacement DECONTAMINATION & RINSE Instrument Decontamination & Rinse CHAMBERS ADJUSTMENT Alignment Check and Adjustment of chambers assembly PROBE ADJUSTMENT Probe Home position Check and Adjustment - Probe position Check and Adjustment MAIN BOARD ADJUSTMENT HGB Blank Adjustment - RBC/PLT Gain Adjustment - WBC/BASO Gain Adjustment - Drain sensor Adjustment - LMNE sensor Adjustment Motor Current Adjustment - Thresholds Adjustment TEMPERATURE ADJUSTMENT Reagent heating system temperature Check - Reagent heating system temperature Adjustment - Thermostatedcompartment temperature Check - Thermostated compartment temperature Adjustment VACUUM ADJUSTMENT Draining syringe vacuum Check - Counting syringe vacuum Check Counting syringe vacuum Adjustment BUBBLING ADJUSTMENT Bubbling Adjustment Menu presentation LMNE FLOWCELL Optical Bench Check and Adjustment ADJUSTMENT LMNE BALANCE ADJUSTMENT TUBE HOLDER ADJUSTMENT POWER SUPPLY REPLACEMENT CHECK UP AFTER Instrument check up: Repeatability - Calibration INTERVENTION OPTICAL BENCH DISMANTLING Optical bench Dismantling - Optical bench Replacement - Emission & REPLACEMENT gun location Control - Optical bench lamp alignement Control FRONT PANEL DISMANTLING Front panel Dismantling - Front panel Mounting BARCODE READER Instrument configuration for Barcode reader CONFIGURATION HEATER ASSY REPLACEMENT WORKSTATION SOFTWARE UPGRADE CHANGEMENT OF KEYBOARD TYPE STEP BY STEP ANALYSIS CYCLE
INSTALLATION
8-9
Probe adjustment Procedure

CONCERNS
Check and adjsutment of the probe
REQUIRED TOOLS
None
REQUIRED PRODUCTS
None
INTERVENTION TIME
15 min.
15/02/04
FREQUENCY
On request
SPECIFIC KIT OR CONSUMABLES

None
Pentra 60C+
RAS290B
Probe adjustment
Probe adjustment Introduction Needle home check & adjustment Carriage home adjustment All carriage positions check & adjustment
WARNING
Disposal gloves and white coat must be worn by the operator. Local or national regulations must be applied in all the operations.
Pentra 60C+
Page 2/8
RAS290B
Probe adjustment Introduction
IMPORTANT
Check that the chamber assy is parallel to the carriage motion before checking or making an adjustment of the Probe position. Follow CHAMBERS ADJUSTMENT procedure of this manual.
Most of the probe adjustments can be accessed by the menu Service\Technician Adjustment\Carriage dilution positions as explained below (Diag.1):
Probe adjustment
Needle Home: Adjustment to get the best probe extremity cleaning
Check (2): Checks the deepness of the probe in front of LMNE-3 inlet
Check (1): Allows to check probe cleaning position
Carriage Home: Allows adjustment of the carriage sampling position
Needle position : Probe deepness adjustment (Up/Down)
All Carriage pos.: Probe Left/Right adjustment
Diag.1 Check cycle: Allows to check that probe is aligned with each distribution inlets
IMPORTANT
Check Probe position before any adjustment, running Check functions.
Page 3/8
Probe adjustment
Needle home check & adjustment
IMPORTANT
When the probe is in NEEDLE HOME position it is also in cleaning position. Check and adjust the NEEDLE HOME position to achieve the best cleaning of the probe extrimity
1. Needle Home Check

Open the right access door. Run Service\Technician Adjustment\Carriage dilution positions\Check (1) (Diag.1). Check that the probe is about 1mm out from the percutor (piercing needle) (Diag.2).
Pentra 60C+
Page 4/8
Diag.2
Press ESC to continue.
2. Needle Home Adjustment

Open the right access door. Run Service\Technician Adjustment\Carriage dilution positions\Check (1) (Diag.1). Check that the probe is about 1mm out from the percutor (Diag.2). If Probe is too low (gap>1mm) increase Needle Home number of step. If Probe is too high (gap<1mm) decrease Needle Home number of step. Restart the Workstation. Run the Check (1) cycle again to control the gap.
RAS290B
Carriage home adjustment
IMPORTANT
The CARRIAGE HOME position is a factory made adjustment and must not be changed. It represent a gap of 1.4mm between the tooth wheel and the front of the carriage (as shown on Diag.3) This carriage position is also the sampling position, if you need to readjust the sampling position move the tube holder, but do not change the CARRIAGE HOME value !
Probe adjustment
1.4mm
Diag.3
NOTE
Workstation may have to be restarted after a HOME adjustment.
Page 5/8
Probe adjustment
All carriage positions check & adjustment
IMPORTANT
To check the right position of the probe you must see inlet as in Diagram 4 and not as in diagram 5 (where circles are not concentric).
Diag.4
Diag.5
1. All Carriage Positions Check

Open the right access door
Pentra 60C+
Run Service\Technician Adjustment\Carriage dilution positions\Check cycle (Diag.1) The sampling needle moves over a chamber Check in front of the inlet the right location of the probe: Disconnect the tube connected to the inlet, if the probe is well adjusted you will see it as in the following diagram (Diag.6):
Diag.6 Press ENTER key or click end of the cycle button, to move to the next chamber and continue until the
When the cycle is ended check that no tube is disconnected
2. All Carriage Positions Adjustment
IMPORTANT
Probe Up/Down adjustment is the same for all the chambers and cannot be modified from one chamber to another (adjustment by the NEEDLE POSITION value). Probe Left/Right position in the chambers can be adjusted separately for each chamber (except the Rinse chamber).
Open the right access door Before any adjustment check the probe position in each chamber with the Service\Technician Adjustment\Carriage dilution positions\Check cycle option (Diag.1)
Page 6/8
RAS290B
3. Left/Right adjustment:
Adjust probe position for all the chambers separately in the software cycle order (Diag.7&8)
Probe adjustment
Diag.7
If you want to modify only one of the position, remind to increase or decrease the next motion with the same number of steps, not to loose the other adjustments.
GB (2)
/B
SO
CA
RR
IA
P GE IA
OS
. OS .
L (3) S PO . R CA RIA G
MN S.
A EC
RR
P GE
(1
L1 )DI
CA
IA RR
GE
O EP
2 4
R (4)
BC
3 1
Diag.8
Page 7/8
Probe adjustment
The right location for the probe is when you see it through the inlet as in the following diagram (Diag.9):
Diag.9
When the cycle is ended check that no tube is diconnected
Pentra 60C+
4. Up/Down adjustment:
If the Probe is too high increase Needle position value:
Diag.10
If the Probe is too low decrease Needle position value:
Diag.11
Page 8/8
Changement of keyboard type Procedure

CONCERNS
Changement of keyboard type.
REQUIRED TOOLS
REQUIRED PRODUCTS
None
INTERVENTION TIME
15 min
15/02/04
FREQUENCY
On request

None
Pentra 60C+
None
RAS297B
Changement of keyboard type

Installation
WARNING
Start the Workstation, press ALT+CTRL+DEL to logon. In Logon information window open an Administrator session User name: administrator Password: xxxxx
IMPORTANT
Do not give the administrators password to the customer neither change it. In case of password modification the system may have to be entirely re-installed and all datas will be then lost.
Open Start menu in Taskbar and select Run option (Diag.1):
Pentra 60C+
Diag.1
Diag.2
In Run window enter regedt32 (Diag.2). Select HKEY_USERS on Local Machine(1)window, click over HKEY_USERS(2) directory in this window (Diag.3):
1 2
Diag.3 Page 2/8
RAS297B
Open Registry menu in Toolbar and select Load Hive option (Diag.4):
Diag.4
Select C:\winnt\profiles\abx directory (Diag.5):
Diag.5
IMPORTANT
There are two files named ntuser.dat select the one with upper case initial.
Select Ntuser.dat(1) file and click Open(2) button (Diag.6):
1 2
Diag.6
Enter abx(1) key name and click OK(2) button (Diag.7):
1 2
Diag.7 Page 3/8

A new profile called abx has been created (Diag.8):
Pentra 60C+
Diag.8
Open .DEFAULT(1)\Control Panel(2)\International(3) directorty and double click on Locale : REG_SZ : 00000409(4) in the right side of the window, the String Editor window is opened (Diag.9). Enter keyboard type code in the String Editor(5) window and click OK(6) to validate (Diag.9).
NOTE
The new keyboard is the french standard type, code: 0000040c
1 2
3 5
Diag.9
Page 4/8
RAS297B
Open .DEFAULT(1)\Keyboard Layout(2)\Preload(3) directorty and double click on 1 : REG_SZ : 00000409(4) in the right side of the window, the String Editor window is opened (Diag.10). Enter same keyboard type code in the String Editor(5) window and click OK(6) to validate (Diag.10).
NOTE
The new keyboard is the french standard type, code: 0000040c
1 5 2 3
Diag.10
Open abx(1)\Control Panel(2)\International(3) directorty and double click on Locale : REG_SZ : 00000409(4) in the right side of the window, the String Editor window is opened (Diag.11). Enter same keyboard type code in the String Editor(5) window and click OK(6) to validate (Diag.11).
1 2 4 3 5 6
Diag.11 Page 5/8

Open abx(1)\Keyboard Layout(2)\Preload(3) directorty and double click on 1 : REG_SZ : 00000409(4) in the right side of the window, the String Editor window is opened (Diag.12). Enter same keyboard type code in the String Editor(5) window and click OK(6) to validate (Diag.12).
4 1 5 2 3 6
Pentra 60C+
Diag.12
Close the window session and turn off the computer. Replace the keyboard, connector is shown on Diag.13:
Diag.13
Restart the computer and start a session under user name abx. Check that the keyboard operates normally in the workstation application.
NOTE
As the barcode reader is connected on the keyboard, make sure that the barcode reader works properly after keyboard replacement.
Page 6/8
RAS297B
SPECIAL CASE: Administrator profile
Start the Workstation, press ALT+CTRL+DEL to logon. In Logon information window open an Administrator session User name: administrator Password: xxxxx
NOTE
In the Logon information window the keyboard type is still a QWERTY. To logon with an AZERTY keyboard as an administrator, enter password as if the keyboard was a QWERTY.
Open the Control panel window: Start menu Settings\Control panel (Diag.14):
Diag.14
Run the keyboard program manager (Diag.15):
Diag.15 Page 7/8

Enter Input locales tab and select the new keyboard in the list, click Set as Default (Diag.16) and Apply (Diag.17):
Pentra 60C+
Diag.16
Diag.17
Click OK to close the Keyboard window. Restart the Workstation and check the correct operation of the keyboard.
Page 8/8
Installation Procedure
CONCERNS
Installation of the ABX PENTRA 60 C+ and ABX WORKSTATION.
REQUIRED TOOLS
None
REQUIRED PRODUCTS
None
INTERVENTION TIME
10 min
15/02/04
FREQUENCY
On request

Installation kit: XEA602C
Pentra 60C+
RAS298B
Installation
Installation Instrument installation Workstation installation Start Up Instrument first start up Check up after intervention
WARNING
INSTALLATION KIT XEA602C P/N DESIGNATION
QTY
DAJ007A DBH001A DBH002A DBK003A EAB021A EAB033A EAB035A EAC010A EAC019A EAE005A EAE006A EAE007A EAE008A EAE011A EAE028A EAE034A EBB057AS FAA013A FAA055A FAA057A FBH016A FBL001A GAK302A GBG138A GBG245A GBG145A GBG155A GBG169A MAB001A MAB002A MAB018A MAB090A XDA483B XDA621A XEA018A XEA019A XDA692A XDA693A
LAMPE 20W 9.5V TYRAP Lg=92 TYRAP Lg=140 FLAT CABLE ADHESIVE SUPPORT RACCORD Y =3mm RACCORD T =1.6mm T410-6 RACCORD T =2.3mm T220-6 LUER FEM. I=3mm LUER MAL. I=3mm TYGON TUBE 1.02mm (0.040) TYGON TUBE 1.295mm (0.051) TYGON TUBE 1.52mm (0.060) TYGON TUBE 2.06mm (0.081) CRISTAL TUBE 3x6 CRISTAL TUBE 4x6 TYGON TUBE 2.54mm (0.100) FILTER ORING 1.8x1.2 ORING 1.07x1.27 ORING 5x1 PENTRA 60 COVER RUBBER STOPPER (2 HOLES) STOPPER 1/4 TURN DOOR KEY REAGENT STRAW STOPPER =28 REAGENT STRAW STOPPER =20 CAPSULE =25 PERCUTOR ALLEN BENT KEY 2mm ALLEN BENT KEY 2.5mm ALLEN BENT KEY 3mm TORX BENT KEY T10 3v LIQUID VALVE (WITHOUT SELF) ORING 30.8x3.8 + WASHER DILUENT STRAW GREASE DILUENT INPUT TUBING WASTE OUTPUT TUBING
1 3 3 1 1 1 1 2 1 2 ml 2 ml 2 ml 2 ml 2 ml 2 ml 2 ml 1 1 1 1 1 2 3 1 3 1 1 1 1 1 1 1 1 1 1 1 1 1
Pentra 60C+
Page 2/6
RAS298B
Installation: Instrument installation
Unpack the Instrument. Open the pneumatic door on the right side of the instrument. Remove the 2 plastic parts blocking the carriage for transportation (Diag.1):
Installation
Diag.1
1. Reagent connections
Use Luer connectors, straws and stoppers delivered with the Installation kit to connect Reagents. Bottles & Containers locations (Diag.2): 1 - ABX ALPHALYSE or ABX LYSEBIO 2 - ABX BASOLYSE II 3 - ABX EOSINOFIX 4 - ABX CLEANER 5 - ABX DILUENT (see connector location on Diag.5). 6 - WASTE CONTAINER (see connector location on Diag.5).
IMPORTANT
Risk of erroneous results if diluent container is installed further than 80cm (31.5 in.) bellow the instrument. DILUENT INPUT TUBING: cristal 3x6 / 2 meters (80 in.) maximum. WASTE OUTPUT TUBING: cristal 4x6 / 2 meters (80 in.) maximum. 2. Diluent & Waste containers connection
5 Remove the diluent straw. Install the straw deep in the container. 6
Diag.2 Page 3/6
Installation
3. Bottle connection
Open the reagent front door. Open the reagent bottles and replace the cap by the bottle stoppper and reagent straw assembly, tighten to ensure an adequate seal.. Put the reagent botlles in the compartment and close the door.
Pentra 60C+
Diag.4 Diag.3 4. Electrical connections

Check that the power supply module voltage of the instrument corresponds to the nominal voltage supply of the laboratory and the country. Connect the power supply cable (Diag.5). Connect the RS 232 link to the Worstation (Diag.5).
IMPORTANT
Power requirements: Analyzer and Computer: 400VA Printer (EPSON EPL5700): 700VA
+ Workstation connector + Main supply cable + Diluent input

Cristal tube 3 x 6, length: 2 meters maximum
+ Waste output
Cristal tube 4 x 6, length: 2 meters maximum
Diag.5 Page 4/6
RAS298B
Workstation installation
1.Computer installation
Install the workstation computer next to the ABX PENTRA 60 C+. Check that the power supply module voltage of the computer corresponds to the nominal voltage supply of the laboratory and the country. Connect the power supply cable (Diag.6). Connect the RS 232 link to the ABX PENTRA 60 C+ (Diag.6). Connect all different peripherals: keyboard, mouse, printer, screen (Diag.6).
+ Printer connector + Mouse port

Connect standard mouse to this port.
+ RS Output to LIS
connection
Installation
+ Main supply
From 100Vac to 240Vac.
+ Keyboard/
Barcode connector
Supports standard 105 keys keyboards and barcode reader.
Diag.6
+ Analyzer connector
Connect to ABX PENTRA 60 C+.
+ Screen connector
SVGA output .
Connect the barcode between keyboard and computer:
+ To Computer keyboards input
+ To Keyboard
NOTE
This diagram is given for information, connector locations from one PC to another may be different.
Page 5/6
Installation
2. Printer installation
The default printer is an OKI 4200 (Printer model may change, refer to latest Technical notes for informations about new models). Unpack the printer. Check that the power supply module voltage of the printer corresponds to the nominal voltage supply of the laboratory and the country. Connect the power suppply cable. Connect the parallel cable. Feed the printer with paper.
Pentra 60C+
Page 6/6
Start Up Instrument first start up

1. Start Up & Priming
Check that all is correctly connected. Turn on workstation computer by pressing ON/OFF switch of the computer and screen. After computer boot press ALT+CTRL+DEL (or ALT+CTRL+SUPPR) to start a session. Start the Analyzer. Enter system password and wait until workstation starts. Analyzer indicator turns green if connection is succesfull and reagent levels are updated on the screen. Prime all the reagents: Menu\Hydraulic Systems\Prime Cycles\ All reagents.
2. Check vacuum value

As it depends on altitude the instrument is installed check the vacuum value. Follow procedure RAS314 and eventually adjust vacuum value.
3. Check up after intervention

Follow procedure RAS299 to check instrument accuracy and eventually re-calibrate it.
Check up after intervention Procedure

CONCERNS
Check up and control of the instrument accuracy
REQUIRED TOOLS
None
REQUIRED PRODUCTS
Fresh and normal blood samples Calibration blood samples
INTERVENTION TIME
45 min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
RAS299B
Check up after intervention

Check up after intervention Preliminary Repeatability Calibration
WARNING
Pentra 60C+
Page 2/5
RAS299B
Check up after intervention Preliminary
WARNING
This procedure must be performed on a clean instrument. If the instrument is suspected to not be perfectly clean, perform a Concentrated cleaning.
WARNING
Turn on workstation computer by pressing ON/OFF switch of the computer and screen. After computer boot press ALT+CTRL+DEL to start a session. Start the Analyzer. Enter system password and wait until workstation starts. Run a startup, click button.
Repeatability
The measurement of repeatability is based on the set of 10 results obtained from the consecutive analyses of the same blood sample. CBC or DIFF type analyses can be invoked (combination is not supported) with a limit of 30 results per test. Beyond the 30th result, data generated from a new analysis will be disregarded. To remain undisturbed the CV calculation, the potential results containing defaults generated directly from the analyses channels are rejected. In that case, a dialogue box informs the user: Run several samples in DIFF mode from the Calibration&QC\Repeatability tab and check that obtained coefficient of variation are within the following limits:
PARAMETERS
%CV
TEST LEVEL
WBC RBC Hgb Hct Plt
<2% <2% <1% <1% <5%
at 10x103/L at 5x106/L at 15 g/dL at 45% at 300x103/L
Page 3/5

Calibration
In Calibration & Qc(1)\Calibrators(2) Tab, change Calibrator Lot number(3) according to the Calibrator you are going to use:
1 3
Pentra 60C+
2
Diag.1 Prepare the calibrator according to the specific instructions detailed in the calibrator package insert (temperature, mixing, etc...). Open the vial and locate it in the tube holder. In Calibration & Qc(1)\Calibrators(2) Tab close the tube holder. When the tube holder opens, remove and recap the vial.
IMPORTANT
Risk of erroneous results if the calibrator is not continously mixed between each analysis. Continue mixing the calibrator between each analysis.
When the analysis cycle ends, the first result is displayed on the result chart table. Run another calibrator sample when Green LED turns on.
IMPORTANT
In order to obtain the best possible calibration, it is recommended to run at least 5 calibrator samplings.
The calibration of the ABX PENTRA 60 C+ can be performed on 3 to 11 analyses. The auto-calculation module performs statistics on these results in order to obtain the best calibration coefficients.
Page 4/5
RAS299B
Select results to be involved in the statiscal calculation by clicking checked box in Sel(1) column. To discard a result from the statiscal calculation click again in checked box to remove the mark. The instrument calculates the statistical calibration factors for each parameter.
1 2
Diag.2
If the Coefficient of variation is within the limits setup as described Chapter 4. Instrument configuration and the percentage difference between the target and the mean value is less than 20, instrument will allow auto-calibration: Click on Confirm the changement by clicking (2) to update new calibration coefficients. in the following dialog box:
Diag.3
Page 5/5
Tube holder adjustment Procedure

CONCERNS
XDA654 Tube holder assy replacement Tube holder mechanical adjustments Tube holder software adjustment Tube holder door adjustment Compatible tube list
REQUIRED TOOLS
REQUIRED PRODUCTS
None
INTERVENTION TIME
30 min
15/02/04
FREQUENCY
On request

None
Pentra 60C+
Allen keys Flat screw driver
RAS300B
Tube holder adjustment

XDA654 Tube holder assy replacement Tube holder mechanical adjustments Tube holder parallelism check & adjustment Tube holder position adjustment (Sampling position) Centering of the tube retainer plate Tube holder opening speed adjustment Tube holder software adjustment Introduction Adjustment Tube holders door adjustment Compatible tube list
Pentra 60C+
WARNING
WARNING
The probe is a very fragile part, take care during this procedure not to bend or damage it. If the teflon part of the needle comes into contact with the tube holder, the needle will be damaged.
Page 2/14
RAS300B
XDA 654 Tube holder assy replacement
Remove the front panel (follow the procedure RAS306 Front panel dismantling). Unscrew the lower screws from the tube holders support (Diag.1):
Diag.1
Open and remove the tube holder, then unscrew the 2 top screws from the tube holders support (Diag.2):
Diag.2
Remove the plastic collar (Diag.3) and disconnect all the wires from tube holder:
Diag.3
Unscrew the 3 screws from the top of tube holders support (Diag.4):
Diag.4 Page 3/14

Connect the new tube holder assy as shown below (Diag.5):
SAMPLING POSITION DETECTION SWITCHES
SW3 SW4 SW5 SW6
E10 E12 E14 E16 START CYCLE SWITCH
Pentra 60C+
ELECTRO-MAGNET COMMAND E7
Diag.5
E8 SW7 E18
Install the new tube holder assy and tube retainer plate (Diag.1,2&4). Locate the screws but do not tighten them before you adjust tube holder position. Replace the plastic collar (Diag.3).
Page 4/14
RAS300B
Tube holder mechanical checks & adjustments Tube holder parallelism check & adjustment
Check tube holder parallelism with a key: Put the key across the tube holder and compare the gap up and down between the tube holder and the key (Diag.6&7):
Diag.6
Diag.7
NOTE
The gap must be nearly equal all along the tube holder. If necessary adjust tube holder parallelism by untightening the lockers screws (Diag.8) and adjusting locker.
Diag.8
Once parallelism is adjusted, push backwards the tube holder (closed position) and check presence of a 1 or 2 mm. play (Diag.9):
IMPORTANT
Without this play the tube holder will not open correctly.
Diag.9 Page 5/14

If necessary untight the 4 screws (Diag.10-1&11-1) and adjust behind tube holder the tube holder stopper (Diag.12-7) to get the right adjustment.
Diag.10
Pentra 60C+
Diag.11
Diag.12
Page 6/14
RAS300B
Tube holders position adjustment (Sampling position)
Switch on workstation computer by pressing ON/OFF switch off the computer and screen. After computer boot, press ALT+CTRL+DEL (or ALT+CTRL+SUPPR) to start a session. Start the Analyzer. Enter system password and wait for workstation start. Run an Initialization from Menu:\Mechanical systems\Initialization\Run to put the carriage in sampling position. Switch off the Pentra 60C+, remove the tube holder, then untight all the tube holders support screws (Diag.13,14&17):
Diag.13
Diag.14
Put the tube holder and close the door (beware to the probe). Push down the probe into one hole, adjust left/right position of the tube holder to center the probe in the hole by moving the whole tube holder assy (Diag.15):
Diag.15
Lift the probe and open tube holder. Tight gently the 4 screws (do not tight too much the screws).
Page 7/14

Adjust forward/backward position of the tube holder: Unscrew the tube holders axis to center the probe in one of the tube holders hole (Diag.15&16):
Diag.16
Tight the axis.
Pentra 60C+
Centering of the tube retainer plate

Untight the 3 screws from the top of the tube holders support (Diag.17):
Diag.17
Adjust the tube retainer plate to be centered with probe (Diag.18):
Diag.18
Tight gently the 3 screws from the top of the tube holders support (Diag.17).
Page 8/14
RAS300B
Tube holders opening speed adjustment
NOTE
This adjustment must done instrument fitted with door and tube holder.
Switch off the instrument Close the door and open it, as shown on diagram 16, to control the opening speed:
Diag.19
If necessary untight the screw (Diag.20-1) and adjust the spin to get an acceptable speed:
Faster
IMPORTANT
If opening speed is too fast the tube risks to be ejected. Once the adjustment is done, make
1
Diag.20
sure that the screw (Diag.20-1) is well tightened.
Page 9/14

Tube holder software adjustment Introduction
From menu System\Technician Adjustment(1)\Sampling needle adjustment(2) (Diag.21), the Adjustment function returns the number of steps between the Needle in home position and the top of the holder:
1 2
Pentra 60C+
Diag.21
Adjustment: Returns the number of steps between the Needle in home position and the top of the holder.
Control: Check the number of steps between the Needle in home position and the top of the holder.
This value is stored in the Tube holder position field in menu System\Mechanical Systems(1)\Sampling needle adjustment(2) (Diag.22).
1 2
Diag.22 Page 10/14
RAS300B
Adjustment
Switch on workstation computer by pressing ON/OFF switch off the computer and screen. After computer boot press ALT+CTRL+DEL (or ALT+CTRL+SUPPR) to start a session. Start the Analyzer. Enter system password and wait until workstation starts. Remove the tube holders door (Diag.29). Close the tube holder. Enter menu System\Technician Adjustment(1)\Sampling needle adjustment(2) and click Control(3) button (Diag.23), the needle moves down to the level of a tube position. .
Diag.23
CAUTION
If the teflon part of the needle comes into contact with the tube holder, the needle will be damaged. Instrument running be carefull during intervention.
Gently press and turn the tube holder (Diag.24), check that the needle nearly touch the surface of the tube holder without any contact. Turn the tube holder to its original position. If the adjustment is correct, click on OK, the needle moves up. If not: Click on OK, the needle moves up, modify the number of steps in the menu System\Mechanical Systems(1)\Sampling needle adjustment(2) (Diag.22) Tube holder position field. Increase number of steps if the needle is above the tube holder, decrease number of step if the needle is too low inside the tube holder. Click on enter then escape to validate the value. Return in the menu sampling needle adjustment and rerun the control cycle (Diag.23).
Diag.24 Page 11/14

Tube holders door adjustment
After a complete tube holder assy adjustment the door may have to be adjusted. Install the front panel (follow the procedure RAS306 Front panel dismantling). Start the instrument.
CAUTION
Instrument running be carefull during intervention.
Remove the tube holder and close the door smoothly, do not lock it, control where the door jams. Remove the door (Diag.25):
Pentra 60C+
Diag.25
Enter Menu\Mechanical systems\Sampling needle adjustment, click Id holder position button and maintain the tube holder for few seconds closed, to lock it in this position. Release the 2 screws (Diag.26-1) and adjust the door (Diag.26-2) according to the previous control:
1
Diag.26
Gently tight one of the screws, open the tube holder and fit the door. Control again the door position, repeat until you get an acceptable adjustment of the door.
Page 12/14
RAS300B
Compatible tube list
From menu System\Mechanical Systems(1)\Sampling needle adjustment(2) you can setup parameters for each sampling position (Diag.27): Level: Sampling level (from the bottom of each tube holders hole, in mm.).
1 2
Diag.27
Depth: Hole depth in mm. Standard values depending on the tube holder. Do not modify. Id holder position: Returns in Position Id. field the tube holder position after you close it.
Tube holder position: Number of steps between the needle home position and the top of the tube holder (about 400 steps).
Sampling positions are as follows (Diag.28):
3
Diag.28
See compatible tube list on next page.
Page 13/14
POSITION 1
PIERCIENG MAN.
CONDITIONS OF CAP
MODEL
P/N
ADDITIVE
VOL. VACUUM STICKERS
TYPE
OBSERVATIONS
Becton D Vacutainer 368452 K3-EDTA Becton D Vacutainer 367651 K3-EDTA Becton D Vacutainer 367856 K3-EDTA Becton D Vacutainer 367652 K3-EDTA Becton D Vacutainer 367654 K3-EDTA Terumo Venoject II VP-053SDK K3-EDTA Terumo Venoject VT-050STK K3-EDTA Terumo Venoject VT-053STK K3-EDTA CML ABX 3004002 TH5C0C K3-EDTA Greiner Vacuette 454087 K3-EDTA Greiner Vacuette 454086 K3-EDTA Greiner Vacuette 454036 K3-EDTA Greiner Vacuette 454223 K3-EDTA LDM Paris EDTAKE
POSITION 2
5ml Man+BC 5ml 2ml Man+BC 5ml 3ml Man+BC 5ml 3ml Man+BC 5ml 4,5ml Man+BC 5ml 3ml Man 5ml 5ml Man 5ml 3ml Man 5ml 4ml Man+BC 5ml 2ml Man+BC 5ml 3ml Man+BC 5ml 4ml Man+BC 5ml 4,5ml Man+BC 5ml 4,5ml Man+BC 3ml 3ml 3ml 5ml 2,2ml 0,5ml 0,5ml 0,5ml Man Man *without cap with cap without cap *out of format without cap *out of format without cap *out of format without cap
with cap with cap with cap with cap with cap with cap with cap with cap with cap with cap with cap with cap with cap with cap
rubber with groove Hmogard Hmogard Hmogard Hmogard UltraSeal rubber with groove rubber with groove rubber strongly not advisable Hmogard Hmogard Hmogard Hmogard Hmogard rubber strongly not advisable rubber strongly not advisable with thread unlostable unlostable ** Microgard
Becton D Vacutainer
6385 VT-030STK K3-EDTA
K3-EDTA
*Because of the cap thickness and the lack of space between the holder and the top of the tube, the tube holder may not open correctly.

Terumo
POSITION 3
Venoject
Comar R&D SystemsTX2B 18533 IF

POSITION 4
Sarstedt 901091 KABE ABX 30010010777008RED

POSITION 5
*The tube accepts a small sticker (not supplied by the manufacturer) *The tube accepts a small sticker (not supplied by the manufacturer)
Becton D Microtainer
POSITION 6
365975
*The tube accepts a small sticker (not supplied by the manufacturer) **Cap fitted with an adaptator (other probe adjustment) 365973 0,5ml *out of format without cap
GBG 214 AS Standard tube holder positions: Position 1, position 2, position 3, position 4 GBG 215 AS Optional tube holder positions: Position 1, position 3, position 5, position 6
Becton D Microtainer
Man: Manufacturer BC: Barcode
*The tube accepts a small sticker (not supplied by the manufacturer)
Page 14/14
Pentra 60C+
LMNE Balance adjustment Procedure

CONCERNS
LMNE Balance calibration and forced calibration.
REQUIRED TOOLS
REQUIRED PRODUCTS
Fresh and normal blood samples (5 different samples at least)
INTERVENTION TIME
15 min
15/02/04
FREQUENCY
On request

None
Pentra 60C+
None
RAS303B
LMNE Balance adjustment

LMNE Balance adjustment Introduction
WARNING
WARNING
Pentra 60C+
Turn on workstation computer by pressing ON/OFF switch of the computer and screen. After computer boot press ALT+CTRL+DEL (or ALT+CTRL+SUPPR) to start a session. Start the Analyzer. Enter system password and wait until workstation starts. Run a startup, click button.
Calibration
Enter menu Technician Measurement(1)\LMNE Calibration(2) (Diag.1).
1 2
Diag.1
Page 2/4
RAS303B
Prepare a minimum of 5 different human blood samples without WBC alarm, insert one of the tube and close the tube holder. When the analysis cycle ends, the first result is displayed on the result chart table. When the Green LED turns on, run another specimen, and so on until you get a minimum of 5 different results.
NOTE
At least 5 results are necessary to calibrate the LMNE Balance.

Select results to be involved in the statistical calculation by clicking the checked box in Sel(1) column. To discard a result from the statistical calculation click the checked box again to remove the mark. The auto-calculation module performs statistics on these results in order to obtain the best calibration coefficient.
NOTE
The variation between the old and the new coefficient must be within 20% or the calibration would fail.
Click
(2) button to apply new calibration coefficient (Diag.2):
Diag.2
Page 3/4

Confirm new coefficient by clicking OK(1) in the following box (Diag.3):
Pentra 60C+
Diag.3
New LMNE Balance coefficient is applied to the instrument.
Forced calibration
NOTE
LMNE Balance coefficient can be modified dircetly in the software (Forced calibration). Enter System\Restricted tab and modify LMNE Coef. value (1).
Diag.4 Page 4/4
Temperature adjustment Procedure

CONCERNS
Heating coil and Thermostatic compartment temperature check & Adjustment.
REQUIRED TOOLS
REQUIRED PRODUCTS
None
INTERVENTION TIME
30 min
15/02/04
FREQUENCY
On request

None
Pentra 60C+
Thermometer
RAS305B
Temperature adjustment
Heating coil Heating coil temperature check Heating coil temperature adjustment Thermostatic compartment Thermostatic compartment temperature check Thermostatic compartment temperature adjustment
WARNING
Pentra 60C+
Page 2/8
RAS305B
Heating coil Heating coil temperature check
Concerns the control of the Heating coil temperature (reagent heating system). Start the instrument. Enter Menu: Service\Technician adjustment(1)\Heating coil adjustment(2) (Diag.1)
1 2
Diag.1
+ Reference CTN value

Capture the CTN constant in this field.
+ Run Cycle Number

Number of cycles to check the temperature adjustment.
+ Consigne temperature
Allows the modification of the temperature instructions.
+ Run
Starts the temperature adjustment check cycle (The number of cycles to run depends on the Run Cycle Number).
WARNING
This control must be made with the right side door closed and under stabilised thermic conditions (5min at least after startup)
Page 3/8
Open the right side of the instrument and plunge the thermometer probe deeply in the LMNE chambers inlet 1 (Diag.2):
IMPORTANT
Make sure you have plunged the thermometer probe deeply Diag.2 into the liquid.
Pentra 60C+
Close the right side door. Enter in Run Cycle Number(1) (Diag.3) 5 cycles to run.
Diag.3
Click Run(2) button (Diag.3) and check that temperature is aroud 35C +/-1C every time you ear a bip.
NOTE
Check is validated if you get during control at least 10 times the right temperature. If an adjustment is required, when heating block has been replaced for example, follow the procedure. Page 4/8
RAS305B
Heating coil temperature adjustment
Check and eventualy change the CTN value.
+ A LED near the connector shows the

heating command: - when lit off, heating is off - when blinking, heating is on - when lit on, heating is maximum
Diag.4
NOTE
The Heating coil CTN value is located on the CTN wire, close to the main board, connected on J31 (Diag.4). Be sure to read the right value, the sticker may be up or down.
Make a Heating coil temperature check (previous page of this procedure). If you dont get the right temperature, adjust it by the mean of the Consigne temperature (Diag.1).
NOTE
Consigne temperature must be a rounded value 34, 35, 36... When Consigne temperature increases, reagent preheating temperature increases. It may be necessary to restart the entire system after a Consigne temperature changement.
Make a Heating coil temperature check as described at the begining of the procedure. If required change the Consigne temperature value and check temperature again, until you get an acceptable temperature.
Page 5/8
Thermostatic copartment Thermostatic compartment temperature check
Concerns the control of the Thermostatic compartment temperature. Start the instrument. Enter Menu: Service\Technician adjustment(1)\Thermostatic compartment adjustment(2) (Diag.5).
1 2
Pentra 60C+
Diag.5
+ Reference CTN value

Capture the CTN constant in this field.
+ Run Cycle Number

Number of cycles to check the temperature adjustment.
+ Consigne temperature
Allows the modification of the temperature instructions.
+ Run
Starts the temperature adjustment check cycle (The number of cycles to run depends on the Run Cycle Number).
WARNING
This control must be made with the right side door closed and under stabilised thermic conditions (5min at least after startup).
Page 6/8
RAS305B
Open the right side of the instrument and put the thermometer probe in the CTN sensor (Diag.6)
Diag.6
Close the right side door. Enter 5 cycles to run in Run Cycle Number(1) (Diag.7).
Diag.7
Click Run(2) button (Diag.7) and check that temperature is aroud 35C +/-1C every time you ear a bip.
NOTE
Check is validated if you get during control at least 10 times the right temperature. If an adjustment is required, when heating block has been replaced for example, follow the procedure. Page 7/8
Thermostatic compartment temperature adjustment
Check and eventualy change the CTN value, thermostated compartment CTN value is located on the CTN wire, close to the main board, connected on J33 (Diag.8).
A LED near the connector shows the heating command: - when lit off, heating is off - when blinking, heating is on - when lit on, heating is maximum
Pentra 60C+
Diag.8
NOTE
The Thermostatic compartment CTN value is located on the CTN wire, close to the main board, connected on J33 (Diag.8) Be sure to read the right value, the sticker may be up or down.
Make a Thermostated compartment temperature check (previous page of this procedure). If you dont get the right temperature, adjust it by the mean of the Consigne temperature (Diag.7).
NOTE
Consigne temperature must be a rounded value 34, 35, 36... When Consigne temperature increases, Thermostatic compartment temperature increases. It may be necessary to restart the entire system after a Consigne temperature changement.
Make a Thermostated compartment temperature check as described at the begining of the procedure. If required change the Consigne temperature value and check temperature again, until you get an acceptable temperature.
Page 8/8
Front panel dismantling Procedure

CONCERNS
REQUIRED TOOLS
REQUIRED PRODUCTS
None
INTERVENTION TIME
10 min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
Hexagonal key size 3 Flat screw driver Torx screwdriver N10
RAS306B
Front panel dismantling

Front panel Dismantling Mounting
WARNING
Pentra 60C+
Page 2/4
RAS306B
Front panel Dismantling
Switch off the instrument. Disconnect power supply cable. Open the reagent door and remove the 4 bottles (disconnect tubes from the stoppers). Open the pneumatic access door (Right side of the instrument). Open the left side panel and the main board door (lock it).
WARNING
A flat cable is connected at the back of the main board, becarefull to not disconnect it, nor damage it, when you open the door.
Remove the cover. Make sure the probe is lift and push the carriage back. Unscrew the K30x16 torx screw (several turns) (Diag.1&2). Unscrew the 2 CHC M4x6 screws (Diag.1-1):
Diag.1
Diag.2
Page 3/4

Unscrew the 4 CHC M4x10 screws in the reagent compartment (Diag.3):
Diag.3
Pentra 60C+
Lift and pull a little the front panel, to free space in order to disconnect the sampling LEDs command in the internal side of the panel (Diag.4):
Diag.4
Mounting
Reassemble following Dismantling procedure in reverse order.
WARNING
Beware to center the front panel and the tube holders door as precisly as possible. Becarefull not to clamp one of the reagents tube behind the reagent compartment.
Turn on workstation computer by pressing ON/OFF switch of the computer and screen. After computer boot press ALT+CTRL+DEL (or ALT+CTRL+SUPPR) to start a session. Start the Analyzer. Enter system password and wait until workstation starts. Run an Autocontrol cycle to check that all is operating normally and especially the tube holders door.
Page 4/4
6 month maintenance Procedure

CONCERNS
REQUIRED TOOLS
REQUIRED PRODUCTS
Minoclair
INTERVENTION TIME
2 h.
15/02/04
FREQUENCY
See Maintenance table

6 month spare parts maintenance kit: XEA710BS
Pentra 60C+
Hexagonal keys Dynamometric screw driver A302, A301, A300 Cutting pliers Flat screw driver Philips screw driver Torx keys
RAS307B
6 month maintenance
6 month maintenance Rinsing block Reagent syringe Draining & counting syringes Instrument cleaning
WARNING
Pentra 60C+
KIT XEA710BS P/N DESIGNATION
QTY
FAA013A FAA055A FAA057A FAA065A XDA621A XDA622A XEA019A
Oring 1.80x1.20 Nitrile (Rinsing block) Oring 1.07x1.27 Fluorocarbon (Rinsing block) Oring 5x1 Nitrile (Rinsing block) Oring 6.30x2.40 Silicon (Reagent syringe) Oring 30.80x3.60 + Washer (Vacuum/Waste pump) Oring 15.54x2.62 + Washer (Reagent syringe) Silicon grease
1 1 1 1 2 4 1
WARNING
This procedure must be performed on a clean instrument. If the instrument is suspected not to be perfectly clean, perform a Concentrated cleaning. Sampling probe must be in perfect condition in order to get correct MDSS functions.
Page 2/9
RAS307B
6 month maintenance Rinsing block: Oring replacement (1x FAA013A, 1x FAA055A & 1x FAA057A)
Switch off the instrument and disconnect power supply cable. Open the right side door. Move the carriage to access to the rinsing block. Remove the 2 rinsing block screws (Diag.1). Lift the locker (Diag.2) to free the probe and remove the rinsing block.
6 month maintenance
Diag.1
Diag.2
Replace all the Orings from the rinsing block (use a small amount of silicon grease to lubricate FAA057A Oring):
FAA055A
FAA013A
FAA057A
Diag.3
Reassemble in reverse order.
IMPORTANT
Do not tight too much the rinsing block screws.
Page 3/9
6 month maintenance
Reagent syringe: Oring replacement (1x FAA065A & 4x XDA622A)
Drain manually all the syringes before starting operations. Switch off the instrument and disconnect power supply cable. Remove the left side panel and open the main board door (lock it).
WARNING
A flat cable is connected behind the main board, be careful when you open the door not to disconnect nor to damage it.
Disconnect the following tubes (Diag.4): Valve 6 inlet 3, Valve 7 inlet 3, Valve 8 inlet 3, Valve 9 inlet 3, Valve 11 inlet 3.
Pentra 60C+
1
Diag.4 Unscrew the 2 fixation screws and remove carefully the reagents syringe (1). Push several times the pistons above the waste container to drain the syringe.
WARNING
A washer is adapted to each Oring joint thickness, keep the Oring and the washer together. Lyse piston Oring (small one) had no washer.
On a piece of absorbant paper open the reagents syringe (9x CHC M3x12, 2x FX M3x12). Replace all Orings and washers by new one from the 6 month maintenance kit (use one drop of silicon grease in between 2 fingers to lubricate the Orings) (Diag.5):
TIGHTENING TORQUES
9x CHC M3x12: 400mN.m (56.8 Ozf.in) 2x FX M3x12: 400mN.m (56.8 Ozf.in)
Diag.5
Reassemble in reverse order (Use a dynamometric screw driver to tighten the syringe).
Page 4/9
RAS307B
Draining & counting syringes 1- Oring replacement on Draining Syringe (1x XDA621A)
Switch off the instrument. Disconnect power supply cable. Open the pneumatic access door (Right side of the instrument). Remove the back side panel (6x CHC M4x6) (Diag.6).
6 month maintenance
Diag.6
Disconnect electrical connectors behind the instrument (Diag.7).
Diag.7
Diag.8
Cut the tyraps (Diag.8). Disconnect grounding wire from the frame (Diag.8). Put a piece of aborbant paper under the syringe and disconnect both tube.
Page 5/9
6 month maintenance
Diag.10
Diag.9
Pentra 60C+
Unscrew the 4 CHC M4x16 syringe screws (just few turns to free silent block) (Diag.9). Remove the syringe. Drain the syringe pushing the piston nut up and down several times. According to the type of syringe fitted, unscrew the 4x CHC M4x16 screws (Diag.10) or unscrew the syringe body and open it (Diag.11).
Diag.11
Diag.12
Replace the Oring (Diag.13) by the new one from the 6 month maintenance kit (use one drop of silicon grease in between 2 fingers to lubricate Oring and piston). Reassemble in reverse order according to diagram 12 (Molded syringe body Diag.11 has to be rotated until the mark on the body is located between the two marks of the motor Diag.14).
Anti-extrusion ring
Oring Oring thickness wedge
Diag.13
Diag.14
Page 6/9
RAS307B
2- Oring replacement on Counting Syringe (1xXDA 621 A)
Switch off the instrument. Disconnect power supply cable. Remove the left side panel and open the mian board door (lock it).
WARNING
A flat cable is connected behind the main board, be careful when you open the door not to disconnect or damage it.
Remove the back side panel.
6 month maintenance
Disconnect the electrical connectors behind the instrument (Diag.15). Unscrew the 4 CHC M4x16 syringe screws (just few turns to free silent block).
Diag.15
Diag.16
Disconnect grounding wire from the frame (Diag.16). Put a piece of absorbant paper under the syringe and disconnect all the tubes from the syringe (Diag.17).
Diag.18
Diag.17
Page 7/9
6 month maintenance
Remove the syringe. Drain the syringe pushing the piston nut up and down several times. According to the type of syringe fitted (Diag.20&21), unscrew the 4x CHC M4x16 screws (Diag.18) or unscrew the syringe body and open it.
Diag.19
Pentra 60C+
Replace the old Oring joint and washer by new one from the 6 month maintenance kit (use one drop of silicon grease in between 2 fingers to lubricate the Oring) (Diag.19).
Diag.20
Diag.21
Reassemble in reverse order according to diagram 22 (Molded syringe body Diag.20 has to be rotated until the mark on the body is located between the two marks of the motor Diag.23).
Anti-extrusion ring
Oring Oring thickness wedge
Diag.22
Diag.23
Page 8/9
RAS307B
Instrument cleaning
Dilute the 12cl bleach to 1 part of bleach for 4 of deionize water (1/5). Instrument environment must be cleaned. No sponge, nor cloth must be used. Only absorbant paper, thrown after use, in contamination bins, have to be used. For small or sensitive assemblies use accurate drier papers. All assemblies suspected to have been in contact with biohazardous material must be disinfected with diluted bleach (the stainless steel must be bleached below 30Celsius). Blood stains or salt marks must be cleaned with spray detergent first. Concerned assemblies: All the thermostated compartment Outer surfaces of the instrument (perpex, covers, reagent locations....) Waste connector plug Liquid valve push button Assemblies close to the needle Tube holder assembly Overflow trays Clean the filter under the rinse chamber (Diag.24) from the pieces of cap rubber:
6 month maintenance
Diag.24
Reinstall all the assemblies and setup the instrument back to its initial configuration. Follow procedure RAS299 to check instrument accuracy and eventually re-calibrate it.
Page 9/9
Yearly maintenance Procedure

CONCERNS
REQUIRED TOOLS
REQUIRED PRODUCTS
Minoclair
INTERVENTION TIME
2 h.
15/02/04
FREQUENCY

Yearly spare parts maintenance kit: XEA486BS
Pentra 60C+
RAS308B
Yearly maintenance
Yearly maintenance Sampling needle Sampling syringe Reagent syringe Draining & counting syringes
WARNING
KIT XEA486BS P/N DESIGNATION
QTY
Pentra 60C+
DAJ007A FAA040A FAA046A FAA064A FAA066A FAA067A GBG275A JAJ007A XEA286AS XEA663A
9.5V 20W lamp Oring 12.10x2.70 Silicon Oring 2.75x1.60 Viton Oring 1.42x1.52 Fluorocarbon Oring 13.10x1.60 Silicon Oring 2.40x1.90 Fluorocarbon Flat EPO joint 0.5 Polyethylene tape Waste chamber Joint + Washer Coaxial kit LMNE flowcell
1 1 2 2 4 4 4 1m 1 1
WARNING
Page 2/13
RAS308B
Yearly maintenance Sampling needle: Needle XDA655DS
Switch off the instrument and disconnect power supply cable. Open the right side door. Pull carriage to access to the probe. Remove the 2 rinsing block screws (Diag.1). Lift the locker (Diag.2) to free the probe and remove the rinsing block.
Yearly maintenance
Diag.1
TORQUE
Diag.2
Rinsing block screws: 120mN.m (17 Ozf.in)
Replace the needle and tight the rinsing block.
Page 3/13
Yearly maintenance
Sampling syringe: Oring replacement (2x FAA064A)
Switch on the instrument Run Menu: Maintenance\Technician\Others\Maintenance carriage position Open the pneumatic access door (Right side of the instrument) Disconnect the diluent tube between valve 18 and sampling syringe (Diag.3) The diluent is drained through the sampling syringe Unscrew both fixation screws of the Sampling syringe on the carriage (Diag.4)
Pentra 60C+
Diag.3
Diag.4
Disconnect from the syringe output tube (Diag.5) Unscrew the 3 FX M3x12 screws from the bottom of the syringe (Diag.6)
Diag.5
Diag.6
Remove the bottom plate (3x FX M3x12), the piston axle and the 2 Oring joints Replace both Oring joints by new one in the yearly maintenance kit (use one drop of silicon grease between 2 fingers for both Oring) Reassemble following previous steps backward When startup and prime are done check that there is no leak
TORQUE
3x FX M3x12: 400mN.m (56.8 Ozf.in) Page 4/13
RAS308B
5DIFF syringe: Oring replacement (1x FAA040A; 4x FAA067A)
Switch off the instrument. Open the left side panel and the mother board door (lock it).
WARNING
A flat cable is connected at the back of the mother board, becareful when you open the door that you dont disconnect nor damage it.
Disconnect the tubes (Diag.7): Valve 2 inlet 2, Valve 3 inlet 3, Valve 5 inlet 1 Unscrew both fixation screws of the 5DIFF syringe (Diag.8) Disconnect gently from the syringe the 2 left tubes.
Yearly maintenance
Diag.7
Diag.8 Remove the bottom plate (2x FX M3x12, 4x CHC M3x12), the piston axles and the Oring joints (Diag.9):
Diag.9
Diag.10
TORQUES
Replace all Oring joints by new one in the yearly maintenance kit (use one drop of silicon grease between 2 fingers for each Oring (Diag.10). Reassemble following previous steps backward. When startup is done check that there is no leak.
4x CHC M3x12: 400mN.m (56.8 Ozf.in) 2x FX M3x12: 400mN.m (56.8 Ozf.in) Page 5/13
Yearly maintenance
Optical bench: Lamp replacement (1x DAJ007A)
Switch off the instrument. Open the cover. Disconnect the lamp supply (Diag.12). Unscrew lamp fixation screws (few turns) (Diag.11).
Pentra 60C+
Diag.11
Diag.12 Turn the lamp and remove it (Diag.12). Change the lamp. Put back the fixation system and block the screws. Reconnect the lamp supply.
WARNING
Do not touch lamp bulbe with the fingers, use a piece of cloth or paper to manipulate the lamp.
Page 6/13
RAS308B
LMNE Flowcell: 1- Coaxial replacement (1x XEA663A)
Switch off the instrument Open the cover Open the left side panel and the mother board door (block it)
WARNING
A flat cable is connected at the back of the mother board, becareful when you open the door that you dont disconnect nor damage it.
Open the optical bench cover (4 screws Diag.13)
Yearly maintenance
Diag.13
Disconnect the T connector (few diluent drops will leak) (Diag.14&15)
Diag.14 Diag.15
Page 7/13
Yearly maintenance
To access ground fitting under the flowcell, unscrew the 2 fixation srews (Diag.16)
Diag.16
Pentra 60C+
Turn slowly the flowcell (take care to the washers) Unscrew the ground screw using a small philips screwdriver (Diag.17)
Diag.17
Put the new LMNE coaxial instead Reassemble following previous steps backward (except optical bench cover) Run Menu: Maintenance\Hydraulics systems\Rinse\Cytometer to get rid of the air bubbles stuck to the inner optical surfaces Check that there is no leak and that flowcell contains no or just a very few air bubbles Put back the optical bench cover
Page 8/13
RAS308B
2- LMNE Flowcell adjustment check
Run Menu: Service\Technician Measurement(1)\Gain Adjustment(2)\LMNE Adjustment(3) (Diag.18). Mix RBC/PLT Latex thoroughly, close tube holder to start sampling. When optical bench is well adjusted:
1 2
Yearly maintenance
Diag.18
Check that the displayed values are within the range. Target values:
PARAMETER
TARGET VALUE
RANGE
LMNE LAMP TRANSFER TIME RESISTIVE CHANNEL ABSORBANCE CHANNEL
6.00 200 50 180
5.50 to 6.50 150 to 250 45 to 55 180 10
If you are out of range perform a RAS311 LMNE Flowcell adjustment procedure.
Page 9/13
Yearly maintenance
Diluent reservoir: Joint & Washer replacement (1x XEA286AS)
Switch on the instrument. Open the pneumatic access door (Right side of the instrument). Run Menu: System\Hydraulics Systems\Drain chambers\Diluent reservoir. When cycle is finished disconnect the drain tube under the reservoir. Unscrew the 2 fixation screws under diluent reservoir (few turns to release diluent reservoir support) (Diag.19):
Pentra 60C+
Diag.19
Lift reservoir, take care that no tube is diconnected from reservoir. Unscrew the 4 reservoir screws on top. Replace joint and washer by new one in the yearly maintenance kit. Locate new washer and joint against the reservoir top, then the diluent reservoir body (Diag.20):
NOTE
Locate reservoir body to have inferior support turned back and tubes in the upper part in front.
Diag.20
Screw the 4 reservoir screws on top. Put back diluent reservoir in its location and block screws. Connect drain tube under diluent reservoir. When startup is done check that there is no leak.
TORQUE
4x CHC M3x20: 120mN.m (17 Ozf.in)
Page 10/13
RAS308B
Draining chamber: Oring replacement (1x FAA066A)
Switch on the instrument. Run Menu: Service\Hydraulics Systems\Drain chambers\All. Open the pneumatic access door (Right side of the instrument). Turn gently the chambers bottom between 2 fingers (Diag.21):
Yearly maintenance
Diag.21
Replace the Oring joint (Diag.22):
Diag.22
Repeat with the other chambers (Diag.23):
Diag.23 When startup is done check that there is no leak.
Page 11/13
Yearly maintenance
Counting Heads: 1- RBC/WBC Coaxial cable Oring replacement (2x FAA046A)
Switch on the instrument. Run Menu: Systems\Hydraulics Systems\Drain Chambers\All. Switch off the instrument. Open the pneumatic access door (Right side of the instrument). Put a piece of white paper or cloth under the Counting chamber assembly. Record the tube positions before dismantling the Counting chamber assembly. Disconnect the chamber tubes (not the wastes). Dismantle the Counting chamber assembly, 3 screws (Diag.24):
Pentra 60C+
Diag.24
Dismantle the electrode loosening the 2 fixation screws (Diag.25):
Diag.25
Diag.26
Use a previously cut micropipette tip to replace the electrode Oring joint as shown in diagram 26. Reassemble electrode. Repeat with the other electrode (Diag.27). When startup is done check that there is no leak.
Page 12/13
RAS308B
2- Counting head aperture joint replacement (4x GBG275A)
Carefully dismantle the counting head (Diag.27) and plunge the aperture in distilled water. Replace both EPO joints by new one (Diag.28). Clean the chamber and the counting head with liquid soap, do not introduce any sharp instrument inside so as to avoid damaging the inside of the chamber and the aperture.
Yearly maintenance
Diag.27
TORQUE
2x CHC M3x12: 120mN.m (17 Ozf.in)
Diag.28
CAUTION
Do not manipulate the aperture using hard instruments. Clean the aperture with a piece of soft paper or preferably, in between 2 fingers.
Rinse thoroughly with distilled water. Dry the exterior of the chamber with a soft paper.
CAUTION
Do not apply too much pressure on the electrode fixation screws, as it can break the aperture (tightening torque = 120mN.m / 17 Ozf.in). It is recommended to reconnect the tubes on the counting head before reassembling the "electrode/chamber/counting head" assy in order to avoid applying constraint on the chamber.
Repeat operations for the other counting head. Reassemble electrode. Repeat with the other electrode (Diag.27). When startup is done check that there is no leak. Follow procedure RAS299 to check instrument accuracy and eventually re-calibrate it.
Page 13/13
Every 2 years maintenance Procedure

CONCERNS
REQUIRED TOOLS
REQUIRED PRODUCTS
Minoclair
INTERVENTION TIME
2 h.
15/02/04
FREQUENCY

Every 2 years spare parts maintenance kit: XEA630CS
Pentra 60C+
RAS309B
Every 2 years maintenance

Every 2 years maintenance Sampling carriage Reagents syringe
KIT XEA630CS P/N DESIGNATION
QTY
Pentra 60C+
GBC030A GBC031A GBG169A GBG260A GBG274A GBG288A KAA009A
Reagent syringe piston Reagent syringe piston Sampling carriage percutor 31mm piston spacer 31mm piston Oring tightener CHC M3 screw
4 1 1 2 2 1 6
WARNING
WARNING
Page 2/8
RAS309B
Every 2 years maintenance Sampling carriage: Percutor, Oring tightener replacement (1x GBG169A, 1x GBG288A)
Switch off the instrument and disconnect power supply cable. Open the right side door. Pull carriage to access to the probe. Remove the 2 rinsing block screws (Diag.1). Lift the locker (Diag.2) to free the probe and remove the rinsing block.
Diag.1
Diag.2
Replace the probe, the percutor and the rinsing block part (Diag.3).
GBG170A
GBG169A
Diag.3
IMPORTANT
Use new Orings when performing 6 Month maintenance at the same time.
TORQUE
Rinsing block screws: 120mN.m (17 Ozf.in)
Page 3/8

Reagents syringe: Pistons (1x GBC031A & 4x GBC030A)
Switch off the instrument and disconnect power supply cable. Remove the left side panel and open the main board door (lock it).
WARNING
A flat cable is connected behind the main board, be careful when you open the door not to disconnect nor damage it.
Disconnect the following tubes (Diag.4): Valve 6 inlet 3, Valve 7 inlet 3, Valve 8 inlet 3, Valve 9 inlet 3, Valve 11 inlet 3.
Pentra 60C+
1
Diag.4
Unscrew the 2 fixation screws and remove carefully the reagents syringe (1). Push several times the pistons above the waste container to drain the syringe. On a piece of absorbant paper open the reagents syringe (9x CHC M3x12, 2x FX M3x12). Replace the pistons.
IMPORTANT
Replace at the same time the Orings and washers from the 6 month maintenance kit and according to the RAS307 procedure.
TIGHTENING TORQUES
9x CHC M3x12: 400mN.m (56.8 Ozf.in) 2x FX M3x12: 400mN.m (56.8 Ozf.in)
Diag.5 Reassemble in reverse order (Use a dynamometric screw driver to tighten the syringe).
Page 4/8
RAS309B
Draining & counting syringes 1- Draining Syringes Piston replacement ((1x GBG274A + 1x GBG260A) or 1x GBG052A)
Switch off the instrument. Disconnect power supply cable. Open the pneumatic access door (Right side of the instrument). Remove the back side panel (6x CHC M4x6) (Diag.6).
Diag.6
Disconnect electrical connectors behind the instrument (Diag.7).
Diag.7
Diag.8
Cut the tyraps (Diag.8). Disconnect grounding wire from the frame (Diag.8). Put a piece of aborbant paper under the syringe and disconnect both tube.
Page 5/8
Diag.10
Diag.9
Pentra 60C+
Unscrew the 4 CHC M4x16 syringe screws (just few turns to free silent block) (Diag.9). Remove the syringe. Drain the syringe pushing the piston nut up and down several times. According to the type of syringe fitted, unscrew the 4x CHC M4x16 screws (Diag.10) or unscrew the syringe body and open it (Diag.11).
Diag.11
Diag.12
Replace the piston according to the syringe model: - Diag.11 replace piston without crosspiece GBG260A (new molded syringe) - Diag.12 replace piston with crosspiece GBG260A (old machined syringe)
IMPORTANT
Use new Orings when performing 6 Month maintenance at the same time.
Reassemble in reverse order (Molded syringe body Diag.11 has to be rotated until the mark on the body is located between the two marks of the motor Diag.13).
Diag.13
Page 6/8
RAS309B
2- Counting Syringes Piston replacement ((1x GBG274A + 1x GBG260A) or 1x GBG052A)
Proceed the same maner as for Draining syringe except for the following points: Disconnect the electrical connectors behind the instrument (Diag.14).
Diag.14 Diag.15
Disconnect grounding wire from the frame (Diag.15). Put a piece of absorbant paper under the syringe and disconnect all the tubes from the syringe (Diag.16):
Diag.16
Page 7/8

Follow procedure RAS299 to check instrument accuracy and eventually re-calibrate it.
Pentra 60C+
Page 8/8
Decontamination & Rinse Procedure

CONCERNS
Instrument decontamination before maintenance operation in the following cases: - Instrument removed from a biohazardous area, - Maintenance intervention on contaminated assemblies. and instrument rinse
REQUIRED TOOLS
REQUIRED PRODUCTS
Fungicidal, bactericidal, virus killing detergent spray, non corrosive for metals, Non plastic altering. Bleach solution 12Cl / Deionize water / Protection gloves / Absorbant paper / Distilled water
INTERVENTION TIME
1h30min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
Hexagonal keys Clamps Flat screw driver Torx keys
RAS310B
Decontamination & Rinse

Decontamination Preparation Manual decontamination Analysis circuit decontamination Drain & Rinse
WARNING
Pentra 60C+
Page 2/6
RAS310B
Decontamination Preparation
Switch on the instrument Open the pneumatic access door (Right side of the instrument) Run Menu: System\Hydraulics Systems(1)\Cleaning Cycles(2)\Concentrated cleaning(3) (Diag.1):
2 3
Diag.1 Confirm by clicking Ok, a rinse cycle is started. Wait until the instrument displays the following screen (Diag.2):
Diag.2 Pour 3ml of Minoclair in each chamber (Diag.3) and click Ok to continue (Diag.2).
Diag.3 When cycle is finished, switch off the instrument and remove the supplying cable Open the instrument cover Spray the bactericidal cleaner on all biohazardous areas and wait for 10 minutes (assemblies in contact with the biohazardous material such as instrument cover, tube holder, keyboard, start key, assemblies close to the SN...
Page 3/6

Manual decontamination
Dilute the 12cl bleach to 1 part of bleach for 4 of deionize water (1/5). Instrument environment must be cleaned and decontaminated. No sponge, nor cloth must be used. Only absorbant paper, thrown after use, in contamination bins, have to be used. For small or sensitive assemblies use accurate drier papers. All assemblies suspected to have been in contact with biohazardous material must be disinfected with diluted bleach (the stainless steel must be bleached below 30Celsius). Blood stains or salt marks must be cleaned with spray detergent first. Concerned assemblies: Outer surfaces of the instrument (perpex, covers, reagent locations....) Waste connector plug Liquid valve push button Assemblies close to the needle Tube holder assembly Overflow trays
Pentra 60C+
Page 4/6
Reinstall all the assemblies and setup the instrument back to its initial configuration.
RAS310B
Analysis circuit decontamination
Prepair 1 bottle containing 1/2 litre of bleach diluted to 1 part of bleach for 9 parts of deionize water (1/ 10). Prepair 1 bottle containing 1/2 litre of distilled water. Switch on the instrument. Replace the reagent bottles by the diluted bleach bottle. Run 6 All reagents prime cycles Menu: Systems\Hydraulic Systems(1)\Prime cycles(2)\All Reagents(3) (Diag.4).
1 2 3
Diag.4
Fill a sample tube with diluted bleach to 1 part of bleach for 4 of deionize water (1/5) Run 15 burn-in cycles(2) Menu: Systems\Burn in cycle(1)\Run burn in(3) (Diag.5). leave the instrument operating until it stops
1 2 3
Diag.5
Page 5/6

Drain & Rinse
Remove the 3 reagent straws from the bottle containing the diluted bleach. Wrap the straws in absorbant paper. Run 2 All reagents prime cycles Menu: Systems\Hydraulic Systems(1)\Prime cycles(2)\All Reagents(3) (Diag.4): the bleach is drained. Replace the diluted bleach by the distilled water bottle and re-plunge the straws in distilled water Run 6 All reagents prime cycles Menu: Systems\Hydraulic Systems(1)\Prime cycles(2)\All Reagents(3) (Diag.4) (Rinse). Remove the 3 reagent straws from the distilled water (Wrap the straws in absorbant paper). Run 2 All reagents prime cycles Menu: Systems\Hydraulic Systems(1)\Prime cycles(2)\All Reagents(3) (Diag.4): the distilled water is drained. Run a STAND BY cycle. Re-install the reagent bottles and the straws.
Pentra 60C+
Switch off the instrument. Close the instrument cover.
WARNING
When rinse is finished check that LMNE chamber is empty.
Page 6/6
LMNE Flowcell adjustment Procedure

CONCERNS
Instrument adjustment: LMNE Flowcell
REQUIRED TOOLS
REQUIRED PRODUCTS
Latex RBC\Plt Fresh blood sample Calibrator
INTERVENTION TIME
1h
15/02/04
FREQUENCY
On request

None
Pentra 60C+
Hexagonal keys Flat screw driver Torx keys
RAS311B
LMNE Flowcell adjustment

LMNE Flowcell Preparation LMNE Flowcell adjustment check LMNE Flowcell position adjustment
WARNING
Pentra 60C+
Page 2/6
RAS311B
LMNE Flowcell Preparation
This adjustment procedure must be carried out when LMNE flowcell is moved or replaced, or when LMNE tube is changed. No adjustment is required when LMNE Lamp is replaced. Menu: Maintenance\Technician\Measurement\LMNE Adjustment allows the adjustment of the entire optical bench parameters. A 1:400th dilution of the RBC/PLT Latex is prepared (5l of Latex, 2ml of diluent) in the LMNE chamber. This dilution is then transfered toward the optical chamber and injected The measurments are continuously displayed every 700ms during a total of 27 seconds.
WARNING
Never dismantle Emission gun or even unlock it. The adjustment is factory made and cannot be performed in the field.
Open the cover. Open the left side panel and the main board door (block it).
WARNING
A flat cable is connected at the back of the mother board, becareful when you open the door that you dont disconnect nor damage it
Diag.1
Run Menu: Systems\Hydraulics systems\Rinse\Rinse cytometer to get rid of air bubbles stuck to the inner optical surfaces. Check that the flowcell contains no or just a very few air bubbles.
Page 3/6

LMNE Flowcell adjustment check
Run Menu: Service\Technician Measurement(1)\Gain Adjustment(2)\LMNE Adjustment(3) (Diag.2). Mix RBC/PLT Latex thoroughly, close tube holder to start sampling. When optical bench is well adjusted:
1 2
Pentra 60C+
Diag.2
Check that the displayed values are within the range. Target values:
PARAMETER
TARGET VALUE
RANGE
LMNE LAMP TRANSFER TIME RESISTIVE CHANNEL ABSORBANCE CHANNEL
6.00 200 50 180
5.50 to 6.50 150 to 250 45 to 55 180 10
If you are out of range or to get a better adjustment follow the procedure.
Page 4/6
RAS311B
LMNE Flowcell position adjustment
Run Menu: Service\Technician Measurement(1)\Gain Adjustment(2)\LMNE Adjustment(3) (Diag.2). Mix RBC/PLT Latex thoroughly, close tube holder to start sampling. Adjust LMNE lamp voltage around 6V: Adjust by R11 potentiometer located on optical bench board (Diag.3).
NOTE
Test points on Optical bench board, near R11 potentiometer, can be used to check 6V with a voltmeter.
Diag.3
Adjust Resistive channel to 50: Adjustment is made by R136 potentiometer located on mother board (Diag.6 on next page). Adjust Absorbance channel to the maximum (and above 170): Adjustment is made by lateral or axial reception gun movement, use toothed wheel (Diag.4) and lateral screw (Diag.5).
Diag.4
Diag.5
NOTE
If you have difficulties to adjust Absorbance, you can increase gain with the R148 potentiometer (Diag.6 on next page). Once you get the highest absorbance adjustment, decrease slowly gain (R148) to get a correct optical gain adjustment with normal fresh blood. Page 5/6

Check that Transfer time is in limits of range (around 200): Transfer time is not adjustable and must be between 150 and 250.
NOTE
Transfer time bargraph is used to ensure the correct Right/Left location of the light rectangle in the flowcell. When the optimal value is reached the transfer time is around 200 because of the constant height between guns alignment and flowcell. This height is guaranteed by the washers under the flowcell, always put them back even when the flowcell is replaced by a new one.
R148
Pentra 60C+
R136
Diag.7 Put back the Optical Bench cover
NOTE
After intervention check, with normal fresh blood, that you have a correct matrix. Page 6/6
Chambers adjustment Procedure

CONCERNS
Instrument adjustment: Chambers assembly adjustment
REQUIRED TOOLS
REQUIRED PRODUCTS
INTERVENTION TIME
15min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
Hexagonal keys Dynamometric screw river A300, A301, A302 Flat screw driver Torx keys
RAS312B
Chambers adjustment
Chambers adjustment Alignment check Alignment adjustment
WARNING
Pentra 60C+
NOTE
It is mandatory to perform the Probe adjustment procedure after this adjustment.
WARNING
Needle extrimity is teflon coated and is a very fragile part. Beware not touch any part with the needle extremity during adjustments.
Page 2/4
RAS312B
Chambers adjustment Alignment check
This adjustment procedure must be carried out when chamber assembly is moved or replaced. Switch off the instrument and disconnect power supply cable. Open the cover and the right access door. Locate probe as shown in diagrams 1 & 2:
Chambers adjustment
Diag.1 Diag.2
Push the carriage backward (without lifting the probe), check that there is the same height between probe and first and last tops of chamber (Diag.3 & 4):
Diag.3
Diag.4
If you need to get a better adjustment follow this procedure.
Page 3/4
Chambers adjustment
Alignment adjustment
Free the chamber support by loosening the 5 screws (Diag.5)
Diag.5
WARNING
Do not touch the chamber with the needle extremity or the needle will be damaged.
Pentra 60C+
Locate probe as shown in diagrams 6 & 7:
Diag.6 Diag.7 Tighten gently the left upper screw. Push the carriage backward (without lifting the probe), check that there is the same height between probe and last top of chamber (Diag.8 & 9)
Diag.8 If necessary adjust position moving up and down chamber Perform an alignment check as described at the begenning of this procedure When adjustment is correct, tighten screws and perform a Probe Adjustment procedure
Diag.9
Page 4/4
Main board adjustment Procedure

CONCERNS
Instrument adjustment: Main board adjustment
REQUIRED TOOLS
REQUIRED PRODUCTS
INTERVENTION TIME
30min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
Hexagonal keys Small Flat screw driver Voltmeter
RAS313B
Main board adjustment

Main board adjustment Hgb blank adjustment RBC\Plt gain adjustment WBC\Baso gain adjustment Drain sensor adjustment Transfer sensor adjustment Motor current adjustment Threshold adjustment
Pentra 60C+
WARNING
Page 2/9
RAS313B
Main board adjustment Hgb blank adjustment
Open the cover and the left access door to main board. Run Menu: Service\Technician Measurment\Gain adjustment\Hgb Blank Adjustment (Diag.1). The utility carries out a rinsing cycle of the WBC chamber and continuously displays the voltage used by the converter. The adjustment cycle lasts for 20 seconds. The adjustment is carried out with the R248 potentiometer (Diag.2). The final adjustment voltage is approximately 4.7 Volts +/- 0.05V. Once adjustment is done, check correct voltage between TP1 and ground.
Diag.1
Diag.2
Page 3/9

RBC\Plt gain adjustment
Open the cover and the left access door to main board A special cycle allows the dilution of the RBC/Plt LATEX to a 1/220th (10 l of LATEX with 2.2 ml of diluent). A special count programme carries out the calculations of the mean volume in the predefined zones and displays them every 700 ms. The duration of the measurement cycle is 21 seconds The adjustments are made using R133 potentiometer for the RBC and R135 for the Plt (Diag.4) Run Menu: Service\Technician Measurment\Gain adjustment\RBC/Plt Gain (Diag.3) Two graphs allow a visual check of the adjustments (Diag.3) Target Values:
RBC=78 / Plt=112
IMPORTANT
Mix RBC/PLT Latex thoroughly before sampling.
Pentra 60C+
Page 4/9
Diag.3
Diag.4
RAS313B
WBC\Baso gain adjustment
Open the cover and the left access door to main board A special cycle allows the dilution of the RBC/Plt LATEX to a 1/733rd (3L of LATEX with 2.2 ml of diluent). A special count programe carries out the calculations of the mean volume in the predefined zones and displays them every 700 ms. The duration of the measurement cycle is 21 seconds The adjustments are made with the aid of a R134 potentiometer (Diag.6) Run Menu: Service\Technician Measurment\Gain adjustment\WBC/BASO Gain (Diag.5) A graph allows a visual assessment of the adjustments (Diag.5) Target Value:
WBC/Baso=102
IMPORTANT
Mix RBC/PLT Latex thoroughly before sampling.
Diag.5
Diag.6
Page 5/9

Drain sensor adjustment
Open the cover and the left access door to main board. Run Menu: Service\Technician Adjustment\Draining sensor adjustment\Run (Diag.7). The utility empties the drain cell in order to adjust the voltage to 4.5Volts +/- 0.3 (test point TP52, R287 potentiometer on the mother card Diag.8). The utility then fills the sensor with the diluent. check that the voltage on TP52 is less than 1 Volt.
Pentra 60C+
Page 6/9
Diag.7
Diag.8
RAS313B
Transfer sensor adjustment
Open the cover and the left access door to main board Run Menu: Service\Technician Adjustment\Transfer sensor adjustment\Run (Diag.9) The utility empties the drain cell in order to adjust the voltage to 4.5Volts +/- 0.3 (test point TP48, R286 potentiometer on the mother card Diag.10) The utility then fills the sensor with the diluent. check that the voltage on TP48 is less than 1 Volt.
Diag.9
Diag.10
Page 7/9

Motor current adjustment
Open the cover and the left access door to main board Check and adjust, when necessary, all following voltages: MOTORS TEST POINTS VOLTAGE POTENTIOMETER
Draining syringe Counting syringe Dilutor syringe Optical Bench Injector syringe Horizontal carriage Sample syringe Probe carriage
TP5 TP6 TP7 TP8 TP10 TP11 TP12
4.8 V + 0.05 V 4.8 V + 0.05 V 4 V + 0.05 V 3 V + 0.05 V 3 V + 0.05 V 2 V + 0.05 V 4.5 V + 0.05 V
R149 R150 R151 R152 R154 R155 R156
Pentra 60C+
Potentiometers and Test points are located on Main board as shown on the next diagram:
Draining syringe
Counting syringe
Reagents syringe
Optical Bench Injector syringe
Horizontal carriage
Sample syringe
Probe carriage Up/Down
Page 8/9
RAS313B
Threshold adjustment
Open the cover and the left access door to main board Check and adjust, when necessary, all following voltages: THRESHOLDS TEST POINTS VOLTAGE POTENTIOMETER
BASO RBC PLT LMNE CIS LMNE OD
TP14 TP13 TP2 TP3 TP4
300 mV + 5 300 mV + 5 300 mV + 5 650 mV + 5 350 mV + 5
R157 R158 R159 R160 R161

Diag.18
Potentiometers and Test points are located on Main board as shown on the next diagram:
PLT LMNE OD LMNE CIS RBC BASO
Page 9/9
Vacuum adjustment Procedure

CONCERNS
Instrument adjustment: Concerns counting and draining syringe vacuum control and adjustment.
REQUIRED TOOLS
Barflex
REQUIRED PRODUCTS
INTERVENTION TIME
15min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
RAS314B
Vacuum adjustment
Vacuum adjustment Draining syringe vacuum check Counting syringe vacuum check & adjustment
WARNING
Pentra 60C+
Page 2/5
RAS314B
Vacuum adjustment Draining syringe vacuum check (No adjustment available)
Open the right access door. Disconnect the tube on the draining syringe and replace it by the Barflex tube (Diag.1):
Vacuum adjustment
Diag.1 Enter Menu:Service\Technician Adjustment(1)\Vacuum adjustment(2)\Draining syringe(3) (Diag.2):
1 2 3
Diag.2
Diag.3 Check vacuum on the Barflex, vacuum must be around 260mb and must be stable. Press Ok (Diag.3). Disconnect the Barflex and connect back the tube on the draining syringe. Close the right side door.
Page 3/5
Vacuum adjustment
Counting syringe vacuum check
Open the left side panel and the mother board door (lock it)
WARNING
A flat cable is connected at the back of the mother board, becareful when you open the door that you dont disconnect or damage it
Disconnect the lowest tube on the counting syringe and replace it by the Barflex tube (Diag.4):
Pentra 60C+
Diag.4 Enter Menu:Service\Technician Adjustment(1)\Vacuum adjustment(2)\Counting syringe(3) (Diag.5):
1 2 3
Diag.5 Check vacuum on the Barflex while the following screen is displayed (Diag.6), vacuum must be at 220mb. Press Ok (Diag.6). Disconnect the Barflex and connect back the tube on the counting syringe. Close the right side door.
Diag.6
Page 4/5
RAS314B
Counting syringe vacuum adjustment
Open the left side panel and the mother board door (lock it)
WARNING
A flat cable is connected at the back of the mother board, becareful when you open the door that you dont disconnect or damage it
Disconnect the lowest tube on the counting syringe and replace it by the Barflex tube (Diag.7):
Vacuum adjustment
Diag.7
Enter Menu:Service\Technician Adjustment(1)\Vacuum adjustment(2)\Vacuum value(3) (Diag.8):
1 3 2
Diag.8
Check vacuum on the Barflex while the following screen is displayed, vacuum must be around 220mb. Press Ok (Diag.6). Change the step value. If vacuum is too low increase step value. Repeat vacuum check and step value adjustment until you get the correct vacuum. Disconnect the Barflex and connect back the tube on the counting syringe. Close the right side door.
Page 5/5
Bubbling adjustment Procedure

CONCERNS
Instrument adjustment: Bubbling adjustment.
REQUIRED TOOLS
None
REQUIRED PRODUCTS
None
INTERVENTION TIME
15min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
RAS315B
Bubbling adjustment
Bubbling adjustment Bubbling adjustment
WARNING
Pentra 60C+
Page 2/3
RAS315B
Bubbling adjustment Bubbling adjustment
Bubbling is factory adjusted and normally does not required further adjustment. If an adjustment is necessary follow this procedure. Enter Menu:Service\Technician Adjustment(1)\Bubbling(2) (Diag.1):
1 2
Bubbling adjustment
3 4
Diag.1
Enter new step value for each bubbling(3) (Diag.1) and click Accept(4) (Diag.1).
NOTE
When number of steps rises, bubbling is increasing.
BUBBLING
LOW
LIMIT
NORMAL
HIGH
LIMIT
FIRST DILUTION LMNE WBC/BASO RBC/Plt Hgb
100 100 100 100 300
300 300 300 300 400
400 400 400 400 500
Page 3/3
Step by step analysis cycle Procedure

CONCERNS
Instrument control: Analysis cycle
REQUIRED TOOLS
None
REQUIRED PRODUCTS
Fresh blood sample
INTERVENTION TIME
15 min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
RAS316B
Step by step analysis cycle

Step by step analysis cycle Dismantling Mounting
WARNING
Pentra 60C+
Page 2/6
RAS316B
Step by step analysis cycle introduction A-Introduction
The main cycle consists of four principal phases:
1 - Preparation Of The Sample Phase: Dilutions 2 - Count And Measurement Of The WBC Group 3 - RBC/PLT Group Count 4 - Filling Of Tank
A special cycle is required when no other cycle is started during RBC/PLT count or during the filling up of the tank:
5 - Rinsing Of The RBC/PLT Count Chamber
B-Start cycle condition

Run a startup cycle Needle in the sampling position and green LED lit (Diag.1) All chambers (execpt Rinse chamber) are filled with diluent (Diag.2) Reagents syringe & LMNE syringe down (Diag.3) Draining & Counting syringe down (Diag.3&4)
Diag.2
Diag.1
Draining Syringe Counting Syringe LMNE Syringe Reagents Syringe
Diag.3
Diag.4
Page 3/6

C-Step by step analysis cycle
1 - Preparation Of The Sample Phase: Dilutions
1
First series of dilutions:
Press sampling bar, all the chambers are drained (Draining syringe). Blood sample of 53 l (Sampling syringe). Transfer of the needle toward the rinsing chamber (Sampling needle motor, Carriage motor). Distribution of 3 l of blood during the rinsing of the needle (Sampling syringe, Reagents syringe). Transfer of the needle from rinse chamber to the first dilution chamber (Sampling needle motor, Carriage motor). Distribution of 10 l of blood into a flux of 1.7 ml of diluent (Sampling syringe, Reagents syringe). Transfer of the needle from the first dilution chamber to the WBC and BASO count chamber (Sampling needle motor, Carriage motor).
Pentra 60C+
Distribution of 10 l of blood into a flux of 2 ml of BASOLYSE ll (Sampling syringe, Reagents syringe). Transfer of the needle from the WBC and BASO count chamber to the LMNE mixing chamber (Sampling needle motor, Carriage motor). Distribution of 25 l of blood into a flux of 1 ml of EOSINOFIX (Sampling syringe, Reagents syringe). Transfer of the needle from the LMNE mixing chamber to the rinse chamber (Sampling needle motor, Carriage motor). Distribution of the 5 l of residual blood during the double rinsing (interior and exterior) of the needle (Sampling syringe, Reagents syringe).
REALISATION OF THE LMNE DILUTION

The dilution in the LMNE chamber is completed by 1 ml of diluent after 12 seconds of incubation: Positioning of the needle opposite the distribution nipple for the EOSINOFIX. Control of valve 8 then set the syringe group for a dose of 1 ml. Simultaneously: Setting of the distribution syringe to distribute 25 l of blood. Stop valve 8. Incubation for 12 seconds. Control of valves 1 and 3 and setting of the LMNE injection syringe to distribute 1 ml of the stop diluent. Stop valves 1 and 3.
Page 4/6
RAS316B
Second dilution for the RBC and the Plt:
Transfer of the needle from the rinse chamber to the first dilution chamber (Sampling needle motor, Carriage motor). Sample of 42.5 l of the first dilution (Sampling syringe). Rinsing of needle exterior (in the first dilution chamber) with 0.4 ml of diluent (Reagents syringe). Transfer of the needle from the first dilution chamber to the RBC and Plt count chamber (Sampling needle motor, Carriage motor). Distribution of the 42.5 l of first dilution into a flux of 2 ml of diluent (Sampling needle motor, Carriage motor). Distribution of 0.5 ml of diluent from the interior of the needle (Sampling needle motor, Carriage motor).
NOTE
In the case of this RBC/Plt dilution, it is the entire blood sample that is distributed (and not a portion as in the preceding dilution). The distribution in a flux is kept in order to benefit from the homogenisation aspect of the procedure. In order to attain the hemoglobin measurement we add 0.4 ml of lysing agent into the first dilution chamber immediately following the blood sample for the RBC/Plt dilution.
2 - Count And Measurement Of The WBC Group

LMNE: Loading of the vacuum injection circuit of the optical bench (Draining syringe, control of valves 4 and 5). Start flux in the optical chamber (approximately 2 seconds). Injection, acquisition of the matrix and WBC count for 12 seconds. Simultaneously: WBC and BASO counts: Rinsing of the counting head (Diluter pushed into position, control of valves 9, 10, 21, 23 and 13). Start the vacuum and first part of count (duration of 6 seconds, control of valve 23). Rinsing of counting head and adjustment of the vacuum count. Second part of count (duration of 6 seconds). After this first series of measurements: - Measurement of the hemoglobin. - Rinsing of the WBC/BASOS count chamber with a mixture of 1.06 ml of ABX Cleaner and 1.44 ml of ABX Diluent. - Rinsing of the WBC/BASOS counting head.
Page 5/6

3 - RBC/Plt Group Count
Rinsing of the RBC/Plt counting head. Start the vacuum for the RBC/Plt counting head. First series of counts (duration of 5 seconds, control valve 14). Rinsing of the RBC/Plt counting head and adjustment of the vacuum. Second series of counts (duration of 5 seconds).
NOTE
P.R.F. Process (Permanent Rinse Flow): The WBC/BASOS and the RBC/Plt counting heads are in series during the RBC/Plt count. The count flux from the WBC chamber circulates in the RBC/Plt count head and limits the possibility of the circulation of the RBC behind the count orifice.
Pentra 60C+
Autocleaning of the WBC ruby: The P.R.F. allows as well an auto cleaning of the WBC/BASOS inasmuch as a UNICLEAN/DIL (cleaning solution) mixture is drawn through the WBC/BASO ruby during the entire duration of the RBC and Plt counts.
4 - Filling Of Tank
At the end of the RBC/Plt count, the RBC count vacuum is finished and used for the filling up of the diluent tank (control valves 15 and 25). In order to improve the filling speed, the syringe is initialised for a few seconds then drawn again to complete the filling. A float located in the tank controls the level of liquid.
5 - Rinsing Of The RBC/PLT Count Chamber

For reasons of maximising the mechanical resources, the rinsing of the RBC/Plt chamber is normally programmed for the beginning of the succeeding cycle (which can be started during the RBC and Plt count). If no other cycle is started during RBC/Plt count or during the filling up of the tank, a special rinsing sequence is initiated. The sample signal light changes to red and the instrument is jammed for a few seconds. This sequence involves draining of the RBC chamber followed by a refilling with the diluent.
Page 6/6
Optical bench Dismantling & Replacement Procedure

CONCERNS
Instrument control: Dismantling, Replacement and Control of Optical Bench
REQUIRED TOOLS
REQUIRED PRODUCTS
None
INTERVENTION TIME
30 min.
15/02/04
FREQUENCY
On request

None
Pentra 60C+
Hexagonal keys Dynamometric screw driver A302, A301, A300 Flat screw driver Torx keys
RAS317B
Optical bench Dismantling & Replacement

Optical bench Dismantling & Replacement Control
WARNING
Pentra 60C+
Page 2/6
RAS317B

Switch off the instrument. Disconnect power supply cable. Open the pneumatic access door (Right side of the instrument). Open the left side panel and the mother board door (lock it).
WARNING
A flat cable is connected at the back of the main board, be carefull to not disconnect it, or damage it, when you open the door.
Open the cover. Disconnect tubes from LMNE syringe: - Inlet 1 - Inlet 5 Disconnect tubes from valves: - 1 Inlet 1 - 4 Inlet 2 Disconnect tube from ground fitting (Diag.1)
Diag.1
Disconnect following connections (Diag.2): - Lamp supply. - LMNE CIS & LMNE OD coaxes. - Optical pre-ampli board supply. - Optical bench grounding wire.
Diag.2
Page 3/6

Remove gently the Optical bench. Untighten the silent blocks (5mm allen key) (Diag.3):
Diag.3 Locate new Optical bench. Tighten the silent blocks (5mm allen key) (Diag.3).
Pentra 60C+
NOTE
Do not tighten silent blocks too hard, check that optical bench Cannot be lifted.
Connect all tubing and wiring as previously disconnected.
Page 4/6
RAS317B
Control A-Emission gun location control

Open Optical bench cover (Diag.4):
Diag.4
Check that gap between emission gun and flowcell is around 3mm (Diag.5):
WARNING
Becareful, if you use a 3mm allen key to control gap, that you dont damage flowcell or emission gun
Diag.5
WARNING
This adjustment is factory made and must not be changed
Page 5/6

B-Optical bench lamp alignement control
Open Optical bench cover (Diag.6):
Diag.6 To control lamp put a piece of white paper between reception gun and flowcell (Diag.7):
Pentra 60C+
Diag.7 Check that projection of lamp filament is vertical (Diag.8):
Diag.9
Diag.8
WARNING
A small screw (1.2mm allen key) allows adjutment of lamp (Diag.9). This adjustment is factory made and must not be changed.
Page 6/6
Barcode reader configuration Procedure

CONCERNS
Instrument adjustment: Barcode Reader test and configuration
REQUIRED TOOLS
None
RAS318B
REQUIRED PRODUCTS
None
INTERVENTION TIME
15min.
21/11/00
FREQUENCY
On request

None
Pentra 6 0 C+
Barcode reader configuration

Barcode reader configuration Barcode reader test Barcode reader setup (DATALOGIC DLC6165-M1 P/n: XBA453A)
WARNING
Pentra 60C+
Page 2/4
RAS318B
Barcode reader configuration Barcode reader test
Check that barcode reader is working properly by means of the reading test (Diag.1):
INTERLEAVED 2 OF 5 (no check digit control) with C.D.
1224488 reading 12244881 without C.D.
121314151617 reading 121314151617
CODE 39 (no check digit control) with C.D.
reading 12345ABCDE without C.D.
12345ABCDE reading 12345ABCDEW
CODE 128 (control without check digit transmission)
CODABAR (no egality control between start/stop character)
37859 reading 37859
Diag.1
123456/$ reading 123456/$
Page 3/4

Barcode reader setup (DATALOGIC DLC6165-M1 P/n: XBA453A)
An audible beep occurs after each reading. Read all the labels from top to bottom and from left to right When the last label is read, check once more on the test labels (Diag.1).
WARNING
For code I2 of 5, to avoid a bad interpretation, it is mandatory to hold the reader in order to read the entire code.
If the reading test has failed for the DATALOGIC reader, proceed to the barcode setup as follows:
$+$* Restore system default configuration
Pentra 60C+
$+ Enter configuration environment
CP507 WEDGE for IBM AT - ALT mode
AZ0 Disable all family codes
AB11AB*0ll6 Code39, no C.D. , 1 to 16 char
AC110416 ITF, no C.D. , 4 to 16 char
AD111AD*0316 Codabar, no start/stop, 3 to 16 char
AI11 Code128, C.D. control without transmission
EA10 no Terminator
ED3 4 good reads before accepting code
$- Exit and save configuration
Page 4/4
Power supply replacement Procedure

CONCERNS
Instrument dismantling: Power supply
REQUIRED TOOLS
REQUIRED PRODUCTS
None
INTERVENTION TIME
30 min.
15/02/04
FREQUENCY
On request

Power supply: DBN 004 A
Pentra 60C+
Hexagonal keys Dynamometric screw driver A302, A301, A300 Flat screw driver
RAS319B
Power supply replacement

Power supply replacement Dismantling & Replacement
WARNING
Pentra 60C+
Page 2/5
RAS319B
Power supply replacement Dismantling & Replacement
Switch off the instrument Disconnect power supply cable Open the left side panel Open the cover Disconnect , on the mother board, the printer RS flat cable in J2 (Diag.1):
Diag.1 With a flat screwdriver disconnect, on the mother board, the supply connector in J37 (Diag.2):
Diag.2 Open the left side panel and the main board door (block it). Disconnect the Optical bench lamp supply cable (Diag.3):
WARNING
A flat cable is connected at the back of the main board, be carefull to not disconnect it, or damage it, when you open the door.
Diag.3
Page 3/5

Unscrew the 2 CHC M3x6 back screws of the power supply (Diag.4):
Diag.4 Unscrew the 2 CHC M3x6 front screws of the power supply (Diag.5):
Pentra 60C+
Diag.5 Remove the power supply.
WARNING
Becareful to the lamp power supply cable when you remove the power supply.
Reassemble following previous steps backward.
Page 4/5
RAS319B
Switch on the instrument. Check following voltages (Diag.6):
TEST
POINT
DESIGNATION
TARGET
TP40 TP41 TP42 TP43
+5V Power supply +12V Power supply +24V Power supply -12V Power supply
+5V +12V +24V -12V
Diag.6
Page 5/5
Heater assy replacement Procedure

CONCERNS
Instrument dismantling: Replacement of the heater assy
REQUIRED TOOLS
REQUIRED PRODUCTS
None
INTERVENTION TIME
1h.
15/02/04
FREQUENCY
On request

Heating coil: XDA625BS
Pentra 60C+
Hexagonal keys Dynamometric screw driver A302, A301, A300 Flat screw driver Torx keys
RAS320B
Heater assy replacement

WARNING
Pentra 60C+
Page 2/8
RAS320B
To access Heater assy you need to remove the Front panel, follow RAS 306 Front panel dismantling of this manual. Pull the Reagents syringe and the LMNE syringe to free space behind (no need to disconnect tubes). Unscrew several turns the 4 silent block screws of each syring (Diag.1):
Diag.1 Unscrew the 2 CHC M3x6 screws shown on next diagram (Diag.2)
Diag.2
Disconnect following tubes on the Chambers assy: - BASO 2 - RBC 1 - LMNE 2 & 3 - DIL 1
Page 3/8

The heater assy is free, let it lay on the frame and by the front aperture disconnect following tubes: - From valve 22_2 (rep.1 on heater) - From valve 8_2 (rep.3 on heater) - From T4 (rep.5 on heater) - From valve 22_1 (rep.7 on heater) - From valve 11_2 (rep.10 on heater)
Diag.3
Pentra 60C+
Page 4/8
The heating system is now as shown on diagram 4:
Diag.4
RAS320B
Disconnect heating system wiring on the mother board plugged in J31: (Diag.5):
Diag.5
Remove the cable through the instrument. Remove the heater assy and drain it over absorbant paper:
Diag.6
Page 5/8

Take new Heater assy and cut tubes as follow: mark: Length: Destination: Exit 1 1.52x260 Valve 22_2 Exit 2 1.52x120 DIL1 chamber inlet 1 Exit 3 1.52x500 Valve 8_2 Exit 4 1.52x120 LMNE chamber inlet 3 Exit 5 1.52x160 side of T4 (2.3) Side of T4 1.52x250 Valve 1_2 Center of T4 1.52x120 Valve 24_1 Exit 6 1.52x120 LMNE chamber inlet 2 Exit 7 1.52x200 Valve 22_1 Exit 8 1.52x120 RBC chamber inlet 1 Exit 9 1.52x60 Exit 11 of heating coil Exit 10 1.52x500 Valve 11_2 Exit 11 1.52x60 Exit 9 of heating coil Exit 12 1.52x120 BASO chamber inlet 2 Note: Already at good length Cut 40mm. Cut 50mm. Cut 40mm. Already at good length Cut 50mm. Already at good length Cut 40mm. Already at good length Cut 40mm. Already at good length Cut 75mm. Already at good length Cut 40mm.
Pentra 60C+
Diag.7
Put new heater on the instrument frame and connect following tubes: FROM TO FROM TO
1 3 5 7 10
Valve 22 Inlet 2 Valve 8 Inlet 2 T4 Valve 22 Inlet 1 Valve 11 Inlet 2
2 4 6 8 9 11 12
Diluent chamber: DIL 1 LMNE chamber: LMNE 3 LMNE chamber: LMNE 2 RBC chamber: RBC 1 rep.11 of the heater rep.9 of the heater BASO chamber: BASO 2
Page 6/8
RAS320B
Dismantle chamber assy to get the tubes through the chambers support (Diag.8):
Diag.8
Lift the heater assy, make the 5 tubes go through the chambers support steel sheet (Diag.9):
Diag.9
Screw back the 2 heater assy screws (Diag.10):
Diag.10
Page 7/8

Put back the chamber assy and connect the following tubes on chambers: - BASO 2 - RBC 1 - LMNE 2 & 3 - DIL 1 Connect heating system wiring on the mother board in J33: (Diag.11):
Pentra 60C+
Diag.11 Push back the Reagents syringe and the LMNE syringe and screw silent blocks (check that no tube is disconnected). Put back the Front panel, follow RAS 306 Front panel dismantling of this manual. Check chambers position, follow RAS 312 Chambers Adjustment of this manual. When startup and prime are done check that there is no leak.
Page 8/8
Pentra 60 C+
TRAINING SLIDES
Pentra 60 C+
EXPLODED VIEWS
Pentra 60 C+
SPARE PART LIST
SPARE PART LIST ...................................................................... 11-2
Pentra 60 C+
SPARE PART LIST
REF DESIGNATION SPECIFIC PACKAGING
CAE006A CAE010A CAX005A CBK043A CBK044A CBK045A CCC006A DAC024A DAC025C DAC029B DAD075A DAD078A DAD112A DAD113A DAJ007A DAL008A DAM009A DBE014A DBN004B DZZ018A EAC008A EAC008A EAC010A EAC010A EAC019A EAE005AS EAE006AS EAE007AS EAE009AS EAE011AS EAE025AS EAE026AS EAE028AS EAE033AS EAE034AS EAE051AS EBB057AS FAA013A FAA017A FAA040A FAA055A FAA057A
SWITCH, MICROSWITCH XC5-81-82 SWITCH, MICROSWITCH XC5-81 PHOTOMETER, HGB PHOTOCELL PCB, QWERTY KEYBOARD P80/P60C+ PCB, MOUSE P80/P60C+/P400 PCB, AZERTY KEYBOARD P80/P60C+ PCB, PC MONITOR P60C+ CABLE, PRINTER MIC/P60/P80 CABLE, L=1.5M FOR SIDE PC P60C+ CABLE, L=2.9M FOR SIDE PC CABLE, LIQUID VALVE L=350 10F14 CABLE, RS232 OUTPUT CABLE, CARRIAGE BOARD P60C+/P80 CABLE, MOTOR RELAY OPTICAL, LAMP FOR BENCH P60/P80 MOTOR, STEPPER MICROS/P60 VALVE, SOLENOID PIERC. BLOCK CABLE, BUSHING D=3, 2 BLACK PCB, POWER SUPPLY BLOCK P60 CABLE, FERRITE FOR COAXIALS FITTING, ANTI ROTATION WASHER FITTING, ANTI ROTATION WASHER FITTING, LUER FEMALE I=3MM FITTING, LUER FEMALE I=3MM FITTING, LUER MALE I=3MM TUBING, TYGON 1, 016(0, 040) L=2M TUBING, TYGON 1, 295(0, 051) L=2M TUBING, TYGON 1, 52(0, 060) L=2M TUBING, TYGON 2, 29(0, 090) L=2M TUBING, CRYSTAL 3X6 L=10M TUBING, SILICON 1, 5X3, 5 L=10M TUBING, SILICON 2X4 L=10M TUBING, CRYSTAL 4X6 L=2M TUBING, TYGON 1, 143(0, 045) L=2M TUBING, TYGON 2.54(0, 100) L=2M TUBING, SILICONE 4, 5X8 L=2M FILTER, RUBBER CAP DEBRIS 13MM ORING, NEEDLE RINSE BLOC C+/80 ORING, TANK MIG+WASTE MIC ORING, 5DIFF SYRINGE D=12, 1 ORING, SAMPL. NEEDLE CT/C+/P80 ORING, PIERC NEEDLE P120/C+/80
11-2
PACK OF 20
PACK OF 5 PACK OF 5 PACK OF 5 PACK OF 5 PACK OF 5
PACK OF 10 PACK OF 10 PACK OF 10 PACK OF 10 PACK OF 10
Spare part list
REF
DESIGNATION
SPECIFIC PACKAGING
FAA064A FAA065A FAA066A FAA067A FAK001A FAK003A FAL009A FAL010A FAM004A FBH016A FBL001A FBR011A FBR012A FZZ024A GAK302A GAL094A GBC015A GBC030A GBC031A GBG003A GBG007A GBG013A GBG022A GBG033A GBG034A GBG035A GBG037A GBG038A GBG040A GBG042A GBG044A GBG045A GBG048A GBG082A GBG093A GBG130A GBG138A GBG145A GBG147A GBG155A GBG157A
ORING, SAMPL. SYRINGE P60/P80 ORING, REAGENT SYRINGE D=6, 3 ORING, DRAINING CHAMBER P60/80 ORING, 5DIFF SYRINGE D=2, 4 CHAMBER, APERTURE 50 CHAMBER, APERTURE 80 SILENT BLOC, FOR SUB ASSEMBLIES SILENT BLOC, OPTIC BENCH P60/80 COVER, PLASTIC FOOT FOR CHASSIS COVER, DUST COVER PENTRA60 REAGENT, REAGENT CAP 2 HOLES BELT, NEEDLE L=364 P60/P80 BELT, CARRIAGE L=544 P60 DOOR, PUSH PUSH P60 REAGENT, BOTTLE STOPPER D=40 CHAMBER, WAST.P120/DIL TANK P60 CLIP, MIX CH. HOLDER MIC/P120 SYRINGE, REAG PISTON P60/SPS/80 SYRINGE, LYSE PISTON MIC/P60/80 CHAMBER, DRAINING 1/4 TURN P60 CHAMBER, DRAINING CHICANE P60 CUP, OVERFLOW T ROOM CHAMBER, T ROOM COVER P60 SYRINGE, REAG BLOC BODY P60/P80 SYRINGE, 5DIFF BLOCK BOTTOM P60 SYRINGE, REAGENT HOLDER PLATE SYRINGE, 5DIFF BLOC BODY P60/80 SYRINGE, 5DIFF BOTTOM PLATE SYRINGE, 5DIFF PISTON P60/P80 SYRINGE, CROSSPIECE P60/SPS/P80 SYRINGE, SAMPL.SYR. BODY P60/80 SYRINGE, SAMPLING BOTTOM PLATE SYRINGE, SAMPLING CROSSPIECE NEEDLE, BLOCK SHAFT MOTOR, PULLEY P60 COVER, TOP COVER P60 DOOR, KEY FOR T ROOM P60 REAGENT, STRAW D=20 P60/P80 CUP, OVERFLOW REAGENTS SYR. P60 REAGENT, BOTTLE STOPPER D=25 CHAMBER, COUNTING HEAD P60/P80
PACK OF 10 PACK OF 10 PACK OF 10 PACK OF 10
PACK OF 3 PACK OF 12 PACK OF 10
PACK OF 4
PACK OF 4
11-3
Pentra 60 C+
GBG158A GBG169A GBG176AS GBG181A GBG188A GBG199A GBG202A GBG214AS GBG215AS GBG218A GBG219A GBG245A GBG272A GBG273A GBG275A GBG292A GBG296A GBG297A HAE026B HAE027B HAE028B HAE029B HAW002A HAW003A HAX0017 LAD002BS MAB090A NAA068A RAA021B RAB092D RAB110B RAB111B RAB112B RAB119B RAB187A RAB188A RAB189A RAC053C XAA423BS XAA427BS XAA429AS XAA472DS
CARRIAGE, HORIZ. STOP BAR P60 NEEDLE, PIERCING P60C+/P80 CARRIAGE, NEEDLE STOP P60C+/P80 CARRIAGE, SAMPLING NEEDLE STOP SAMPLING, SAMPLE TUBE STOP C+ COVER, SAMPLING HOLDER DOOR C+ SAMPLING, DOOR LOCK P60C+/ACTCP SAMPLING, STD TUBE HOLDER P60C+ SAMPLING, OPT TUBE HOLDER P60C+ CARRIAGE, VERTICAL STOP BAR C+ ORING, ANTI EXTRUSION RING REAGENT, STRAW D=28 P60/P80/ACT SYRINGE, WASTE PUMP BODY P60/80 SYRINGE, VACCUM PUMPBODY P60/80 ORING, APERTURE D=0, 5 P60/P80 NEEDLE, PIERC. RINSE BLOC C+/80 NEEDLE, GUIDE P60C+/P80 NEEDLE, RINSING BLOCK P60C+/P80 STICKER, VALVES JOUCO (1-10) STICKER, VALVES JOUCO (11-20) STICKER, VALVES JOUCO (21-30) STICKER, VALVES JOUCO (31-40) STICKER, FOR REAGENT DOOR P60 STICKER, REAGENTS NAMES P60 STICKER, FRONT PANEL P60C+ TOOL, LATEX RBC/PLT (2 x 2ML) TOOL, TORX KEY T10 STICKER, FOR TUBING (169 ITEMS) MANUAL, TECHNICAL P60C+ MANUAL, USERS PENTRA60C+ GB MANUAL, USERS P60C+ I MANUAL, USERS PENTRA60C+ SP MANUAL, USERS PENTRA60C+ D MANUAL, USERS PENTRA60C+ P MANUAL, USERS PENTRA60C+ DN MANUAL, USERS PENTRA60C+ SW MANUAL, USERS PENTRA60C+ G MANUAL, USERS PENTRA60C+ FR PCB, PREAMP+MECA ASSY PENTRA60 PCB, LINK BOARD PENTRA60 PCB, LED BOARD FOR COVER P60/80 PCB, MAIN BOARD P60 OT/C+ V2, 01
11-4
PACK OF 3
PACK OF 12
Spare part list
REF
DESIGNATION
SPECIFIC PACKAGING
XAA504A XAA505A XAA507A XAA508A XAA524A XAA525A XBA144A XBA193A XBA389A XBA390A XBA391A XBA392A XBA393A XBA394A XBA395A XBA396A XBA398B XBA399C XBA403A XBA404A XBA411A XBA421B XBA453A XBA490A XBA547A XBA548A XCA166A XCA167A XCA191A XDA481B XDA483B XDA555AS XDA591AS XDA592AS XDA593AS XDA595AS XDA600A XDA601BS XDA602C XDA605A XDA609B XDA610C
PRINTER, LEXMARK E210 220V PRINTER, LEXMARK E210 110V PRINTER, EPL5900 220V P60C+/P80 PRINTER, EPL5900 110V P60C+/P80 PRINTER, EPL6100 220V PRINTER, EPL6100 110V CABLE, REAGENT GROUND FITTING CABLE, COAX BASO-O.D LMNE-LMNE PHOTOMETER, HB CPTE P60/P80 MOTOR, FOR SYRINGE BLOCKS P60 MOTOR, CARRIAGE PENTRA60 MOTOR, SAMPLING NEEDLE PENTRA60 FAN, MAIN FAN 24V P60/P80/P400 SENSOR, CARRIAGE MOVE PENTRA60 SENSOR, DRAINING DETECTION P60 SENSOR, SAMP NEEDLE MOVE P60/80 CABLE, COAX RBC/WBC P60/C+/P80 FITTING, GROUND FLOWCELL P60/80 TUBING, SHIELD REPARTITOR P60 CABLE, SAMPLING BLOCK P60C+ MOTOR, PIERCING MECHA P60C+/P80 CABLE, LED PCB/MAIN BOARD P60C+ PCB, BARCODE EXTERNAL P60C+/P80 CABLE, LMNE FLOWCEL CATHOD WIRE MOTOR, FOR SAMPLING SYRINGES MOTOR, FOR 5DIFF SYRINGE CHAMBER, ISOLATOR (LONG) CHAMBER, ISOLATOR (SMALL) CHAMBER, ISOLATOR VENT. P80/C+ VALVE, LIQ. 2WAYS/NC W/O COIL VALVE, LIQ. 3WAYS W/O COIL TOOL, FLOWCELL ADJ. KNOB SYRINGE, 5DIFF ASSY P60/P80 SYRINGE, REAGENT ASSY P60/P80 SYRINGE, SAMPLING ASSY P60/P80 MOTOR, BLOCK FOR REAG. SYR. P60 OPTICAL, BENCH PENTRA60 CHAMBER, LMNE FLOWCELL P60/P80 CHAMBER, 4 CHAMBERS BLOC P60/80 CHAMBER, DIL. TANK ASSY P60/P80 SAMPLING, MECHANISM ASSY P60C+ CHAMBER, WBC/BASO CPTE P60/P80
11-5
Pentra 60 C+
XDA611C XDA612CS XDA613CS XDA614CS XDA615CS XDA616AS XDA617AS XDA621A XDA622A XDA623AS XDA624AS XDA625AS XDA625BS XDA626AS XDA629AS XDA644AS XDA654A XDA655BS XDA655DS XDA657A XDA657C XDA692A XDA693A XDA725A XDA725B XDA756CS XDA757AS XDA758AS XDA759AS XDA812A XDA824AS XDA826AS XDA827AS XDA828AS XDA832AS XDA834AS XDA930A XEA018A XEA286AS XEA381A XEA410AS XEA486BS
VALVE, LIQ 11 VALVE ASSY (1-11) VALVE, LIQ 5 VALVE ASSY (12-16) VALVE, LIQ 2 VALVE ASSY (17-18) VALVE, LIQ 7 VALVE ASSY (20-26) VALVE, LIQ 5 VALVE ASSY (27-31) NEEDLE, 190L SYR. P60/SPS/P80 NEEDLE, 100L SAMPL SYR. P60/80 ORING, VAC/WASTE PUMP + WASHER ORING, REAGENT SYR. + WASHER REAGENT, BLOCK EQUIPED P60/P80 DOOR, FOR T ROOM EQUIPPED P60 HEATER, BLOCK COMPLETE P60/P80 HEATER, BLOCK COMPLETE P60/P80 CHAMBER, DIL.TANK COVER P60/P80 COVER, REAGENT DOOR EQUIPED P60 COVER, LEFT SIDE EQUIPED P60 SAMPLING, PIERCING BLOCK P60C+ NEEDLE, SAMPL. NEEDLE P60C+/P80 NEEDLE, TEFLON SAMPL. NEEDLE P60C+/P80 CHAMBER, 5 CHAMBERS BLOCK P60 CHAMBER, 5 CHAMBERS BLOC P60/80 TUBING, DILUENT ASPIRATION P60 TUBING, WASTE P60 SAMPLING, MECHANISM ASSY P80/C+ SAMPLING, MECHANISM ASSY P80/C+ NEW CAME VALVE, LIQ 4 VALVE ASSY (17-32) CARRIAGE, NEEDLE CARRIAGE P60C+ VALVE, SOLENOID PIERC. BLOC C+ COVER, FRONT COVER EQUIPED C+ KIT, T ROOM FAN + HARNESS P60 MOTOR, BLOCK SAMPL. SYR.P60/P80 MOTOR, BLOCK 5DIFF SYR. P60/P80 SYRINGE, WASTE CPTE P60/C+/P80 SYRINGE, VACUUM CPTE P60/C+/P80 SYRINGE, 5DIFF BLOCK+MOTOR ASSY SYRINGE, SAMPL BLOCK+MOTOR ASSY CARRIAGE, PIERCING ASSY REAGENT, STRAW DILUENT L=360 KIT, ORING+WASHER P60/P80/P120 GREASE, AIR+LIQ. SYRINGE MICROS KIT, TYGON TUBINGS KIT, MAINTENANCE 1 YEAR P60/P80
11-6
PACK OF 12 PACK OF 10
Spare part list
REF
DESIGNATION
SPECIFIC PACKAGING
XEA486CS XEA487AS XEA488AS XEA602C XEA616AS XEA629AS XEA630CS XEA663A XEA665AS XEA701AS XEA708AS XEA710BS XEA726BS XEA727AS XEA747AS XEA770AS
KIT, MAINTENANCE 1 YEAR P60/P80 KIT, T ROOM FAN+HARNESS P60 KIT, HEATER HARNESS P60/P80 KIT, INSTALLATION P60C+ KIT, FLOWCELL INJECTOR TUBE P60 KIT, MAINT. P60 C+ 6 MONTHS KIT, PISTONS + NEEDLE P60C+ CABLE, COAX LMNE FLOWCEL P60/80 KIT, V1.61 PC P60C+ KIT, V1.7 PC P60 C+ KIT, SOFT SETUP PC FOR P60C+ KIT, MAINT.6 MONTHS P60/P80 KIT, V2.21PC + V2, 11 SYST P60C+ KIT, PC MEMORY EXTENSION P60C+ KIT, PISTON + CROSS PIECE KIT, V2.29PC + V2, 16 SYST P60C+
11-7

ABX Pentra 60-C Plus Analyzer - Service Manual

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ABX Pentra 60-C Plus Analyzer - Service Manual

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Pentra 60C+

Copyright 2004 by ABX DIAGNOSTICS

TECHNICAL NOTE SOFTWARE REVISION

1.2. Limited garantee

1.3. Safety precautions, electronic and moving parts

1.4. Biological risks

1.5. Instrument cleaning

Instrument internal cleaning

1.6. Graphics and symbols

In Vitro Diagnostics medical device

Caution, consult accompanying documents

Fragile, handle with care

Consult Instructions for use

2.4. Humidity and temperature conditions

2.5. Electromagnetic environment check

2.6. Environment protection

2.7. Transport and storage conditions

SPARE PART LIST .......................................................................................... XXI

1.1.2. CBC + 5DIFF Mode

STD SI mmol/l JAPAN

WBC 103/mm3 109/l 109/l 102/mm3 MCH pg pg fmol pg LYC % % % % EOS # # # #

HGB g/dl g/l mmol/l g/dl RDW % % % % MON % % % % BAS # # # #

HCT % l/l l/l % MPV m3 fl fl m3 MON # # # # ALY % % % %

MCV m3 fl fl m3 PDW % % % % NEU # # # # LIC % % % %

STD SI mmol/l JAPAN STD SI mmol/l JAPAN

STD SI mmol/l JAPAN

To select the set of units refers to Chapter 4. Instrument configuration

1.2. Throughput Analyses

1.3. Tube Identification

1.5. Workstation Computer

1.6. Measurements and computation

2.1. Power requirements

2.2. Operating temperature and humidity

2.3. Dimensions and Weight

2.4. Minimum specimen volume

2.5. Dilution ratios

2.6. Hgb measurement

2.7. Counting aperture diameters

2.8. Reagent consumption (ml)

2.1 2.1 2.1 X X X X 23.6 X 24 1 X

0.9 0.9 3.7 14 X 24.8 X 1.1 X 25.1 1 1.4

0.4 0.4 1.4 1 X X X X 8.4 8.2 0.3 1

60 60 450 300 310 120 110 220 130 6 135 150

* for one background count only (maxi = 3)

TOTAL PRECISION (SD)

*Source: 510K submission K030144

WBC RBC HGB HCT PLT

10.0 x 103/mL 4.67 x 106/mL 13.6 g/dl 36.0 % 243 x 103/mL

*Source: 510K submission K030144

3.2. Precision (Repeatability)

WBC RBC Plt Hgb Hct

<2% <2% <5% <1% <2%

at 10x103/L at 5x106/L at 300x103/L at 15 g/dL at 45%

0 120 0 8.00 0 24 0 67 0 1900 0 2800

120 150 8.00 18.00 24 30 67 80 1900 2800 2800 3200

0.3 7% 0.07 2% 0.3 2% 2.0 3% 10 10% 10 10%

*Source: 510K submission K030144

Mean low levels Mean high levels Carry over %

1.06 58.81 -0.260

1.58 6.37 0.000

5.28 22.03 -0.179

31.33 1106.67 -0.186

*Source: 510K submission K030144