Velp Scientifica UDK 152 manual

1 - Kjeldahls method to determine the protein content on milk and derived products
the protein content of raw cow milk is meanly 3,2 g/100 ml (512 mg of nitrogen). Human milk is poorer in proteins (1 g/100 ml), while the milk of other animals is richer than cow milk (sheep 5,6 g/100 ml). Procedure 1 Sample: 5 ml (2526 mg of nitrogen in fresh cow milk) in a test tube. 2 Reagents for digestion- for each sample add in the test tube: 7 g anhydrous potassium sulphate (K2SO4) 5 mg Selenium (Se) powder 7 ml sulphuric acid (H2SO4) concentrated 5 ml hydrogen peroxide (H2O2) 35% (130 voll.) 3 Digestion: heat for 30 min at 420C. 4 Cooling: let cool the digestion tubes to 50-60 C. 5 Distillation-titration: place in position the test tube with the digested sample in the unit UDK 152. Select the pre-defined method n. 1 and push START. The analytical parameters memorized in the instrument are: factor: 6.38 Addition of reagents: dilution water=50ml H3BO3=30ml NaOH=50 ml Titrant: HCl 0.1N 6 References: AOAC, Official methods of analysis, method 991.20

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