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Xc nh hm lng trong nguyn li u ! trong "#n $h%m& nh gi #nh h'ng c(a ch ) ch bin xi gc n cht lng "#n $h%m. ). C+ ,- ./ 0"'%10 ).1 V203425 A ).1.1 V32 0678 59':5 9;c * th+c $h%m )ng !,t& !itamin - t.n t/i d0i d/ng cc e"te c(a cc acid b1o b,c cao trong gan& th,n& $h2i ! m3 d+ tr4. * th+c $h%m th+c !,t& !itamin ny t.n t/i d0i d/ng $ro!itamin -. 5rong 6& 78caroten c6 ho/t t9nh !itamin - cao nht& nhng c:ng ch; b<ng =>? lng caroten trong th+c $h%m xut hi n trong c@ thA nh l !itamin d/ng retinol. 5rong c@ thA& !itamin - duy trB tBnh tr/ng bBnh thCng c(a biAu m. Dhi thiu !itamin - & da ! nim m/c Eh& "Fng h6a& !i Ehu%n dG xHm nh,$ gHy !im nhiGm. I6 l cc biAu hi n Eh mJt& Eh gic m/c. Kitamin c6 !ai trL Muan trNng Oi !0i th gic. PJc tO nh/y c#m !0i nh "ng n<m ' !Qng m/c l rodo$"in g.m $rotein ! dRn xut c(a !itamin -. Dhi ti$ xSc !0i nh "ng& rodo$xin $hHn gi#i thnh o$"in T$roteinU ! retinen T-nehy c(a !itamin -U. Dhi mJt ngh;& !itamin - dVn dVn c $hWc h.i tF retinen nhng Ehng hon ton. Xo 6& !i c b2 "ung !itamin - thCng xuyn tF thYc Zn l cVn thit. ).1.) <"=+59 >"?> @?c A5"

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3. N9'%&5 0Nc cO3 >"=+59 >"?> [h@ng $h$ d+a trn Eh# nZng hLa tan c(a caroten trong cc dung mi h4u c@ cho mu !ng& cCng ) mu c(a dung dch t\ l thu,n !0i hm lng Caroten ! o c trn my Muang i n hay Muang $h2 E ' b0c "6ng ]]^ _ ]`^ nm. KB caroten tinh thA dG b oxy h6a trong Ehng Eh9 nn !i c chu%n b thang Cng chu%n thCng "a dWng dung dch Eali bicromat hay abobenben. 5rong dung mi h4u c@ Tete $etrol hay xZngU Ehng ch; carotene m cLn c6 cc "Jc tO nh di $ lWc& xanthocin ! cc cht Ehc c:ng b hLa tan. KB th& cVn thit $h#i tch chSng ra Ehdi caroten b<ng $h@ng $h$ "Jc E9 h$ $hW. P. Q'% 06R5" B=Sc 1T C"'F5 PA Chun b oxyt nhm c m 10% 5hm =^ ml n0c ct !o e^^g oxyt nhm Ehan ! tr)n fu. 5huOc tha c b#o Mu#n trong lN c6 nSt mi ! c "a dWng A $hHn t9ch Ehng "0m h@n m)t ngy "au lSc chu%n b. Chun b dung dch gc kalibicromat gLa tan ^&hig Eali bicromat Tj "y Eh n EhOi lng Ehng 2i ' =]^ oCU trong n0c ct ! a thA t9ch dung dch n !/ch mYc =^^^ml. =ml dung dch ny t@ng Yng theo mu "Jc !0i ^&^^]=?mg Caroten. Xung dch c b#o Mu#n !0i thCi h/n m)t nZm "au Ehi chu%n b. Chun b ct hp ph 3. Ikt !o chl hm$ nht c(a Ong -llin m)t nSt bng hSt %m ! 2 ln 6 ] _ `cm cht h$ $hW n oxyt nhm c6 ) %m =^o. Xpng :a th(y tinh d.n nhm l0$ oxyt xuOng& $h( ln 6 m)t l0$ bng mdng ! 2 ti$ ln = _ icm natri "ul$hat Ehan. qJ$ Ong h$ $hW !o bBnh runden. P. 5r0c Ehi ch/y "Jc Es cVn 2 m)t lng ete $etrol ln trn ! hSt nhm b<ng chHn Ehng cho n Ehi ete $etrol thm 0t fu c)t h$ $hW& ch#y xuOng $h9a d0i ! cLn l/i m)t 9t $h9a trn cht h$ $hW. Chun b thang dung dch chun

a. Xpng buret cho !o cc bBnh nh mYc dung t9ch =^^ml lVn lt =^t i^t u^t ]^t `^ml dung dch gOc Eali bicroamat ! a thA t9ch n !/ch mYc b<ng n0c ct& c%n th,n tr)n fu. 5hCi h/n "a dWng c(a dch chu%n ny Ehng Mu u thng. b. Io m,t ) Muang c(a cc dung dch chu%n trn my "o mu cu!et c6 chifu dy i^ _ u^ mm ' b0c "6ng ]]^ _ ]`^ nm. Xung dch Oi chYng l n0c ct. c. 5F nh4ng Et Mu# o m,t ) Muang thu c& tin hnh xHy d+ng . th chu%n !0i trWc honh l thA t9ch c(a dung dch gOc Eali bicromat ly A chu%n b thang chu%n ! trWc tung l gi tr m,t ) Muang t@ng Yng c(a cc dung dch chu%n. B=Sc )T T215 "$5" vRu c cJt nhd& #o fu trn m#nh nylon ! tF nhifu chl Ehc nhau cHn m)t lng i _ ] g mRu !0i ) ch9nh xc n ^&^`g cho !o cOi "Y j chYa "wn ` _ =^g ct "/ch hay th(y tinh gij nhd. xhanh ch6ng nghifn nt hln h$ trong cOi "Y !0i thCi gian Ehng 9t h@n u _ ] $hSt. IA nh,n c EhOi lng rCi& j Eha n0c c(a mRu cVn thm !o 6 =` _ i`g natri "ulcat Ehan& #o fu. ChuyAn c%n th,n lng mRu j xa ls !o bBnh n6n c6 dung t9ch =^^ _ =`^ml ! r6t !o 6 i^ _ i`ml ete $etrol. yaa cOi chy "Y !i lVn b<ng lng Ehng nhifu ete $etrol ! chuyAn c# !o bBnh n6n. Xpng :a th(y tinh Ehuy fu& ,y nJ$ bBnh l/i A yn i^ ni]h trong b6ng tOi. Pau thCi gian chit "ut& c%n th,n chJt dch chit ln c)t "Jc Es j chu%n b "wn ! tin hnh lNc trong chHn Ehng yu. yaa $hVn cLn l/i trong bBnh n6n b<ng m)t lng Ehng nhifu ete $etrol ! l/i 2 ln c)t. Ki c raa ny c tin hnh !i lVn n Ehi dung dch ch#y ra tr' nn Ehng mu. Io thA t9ch dung dch ete $etrol c6 chYa carotene thu c tF bBnh bunden b<ng Ong ong. Pau 6& o m,t ) Muang c(a dung dch trn my "o mu !0i cu!1t c6 bf dy i^ _ u^mm ' b0c "6ng ]]^ _ ]`^ nm. Xung dch Oi chYng l ete $etrol. 5rong Mu trBnh $hHn t9ch m)t lng ng EA carotene b mt i do tc dWng m/nh c(a nh "ng ! "+ $h h(y c(a Ehng Eh9. KB th& !i c o m,t ) Muang c(a dch chit cVn tin hnh trong Eho#ng thCi gian ngJn nht.

xu m,t ) Muang c(a dch chit l0n h@n ^&? thB cVn $ha lojng b<ng cch ly ch9nh xc i`ml dch chit ny cho !o bBnh nh mYc `^ml ! thm ti$ ete $etrol n !/ch mYc. Dt Mu# trong trCng h$ ny c nhHn ln g$ i. c. T!5" 0C?5 U10 V'W gm lng carotene TXU biAu th b<ng mg trong =Eg mRu c t9nh theo cng thYcz X X Y3 Z M8MMK1[ Z V\Z1MMM] 4 Z P 5rong 6z 3 qng t@ng @ng !0i dung dch gOc tBm thy trn . th chu%n& t9nh b<ng ml M8MM1K[ g "O chuyAn =ml dung dch gOc Eali bicromat ra lng t@ng !0i carotene& mg. V 5hA t9ch dch lNc c(a mRu tha "au Ehi i Mua c)t h$ $hW& t9nh b<ng ml P 4 1MMM 5hA t9ch c(a dung dch chu%n& t9nh b<ng ml DhOi lng c(a mRu tha& t9nh b<ng gt g " chuyn ! =Eg mu.

Dt Mu# cuOi cpng l tr "O trung bBnh c(a hai lVn xc nh "ong "ong. P+ "ai Ehc cho $h1$ gi4a hai lVn xc nh "ong "ong $hW thu)c !o hm lng carotene c6 trong mRu. gm lng Caroten TX=U biAu th b<ng mg trn =Eg ' d/ng !,t cht Eh c t9nh theo cng thYcz 5rong 6z X _ gm lng carotene trong m)t Eg mRu tBnh b<ng mg I) %m c(a mRu& t9nh b<ng o X1 X YX Z1MM\] Y1MM ^ _\

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>"a U1 xguyn li u l mng h/t gc t@i& mng h/t gc "y "@ b) n %m ) =`o. u^^mg nguyn li u j nghifn nhuyGn b<ng chy ! cOi em tr9ch ly !0i =^ ml dung

mi l h nnhexan z acetone Tt\ l uzi !>!U. [hVn ckn c tr9ch l/i u lVn& !0i ` ml dung mi cho m)t lVn. Cc dch chit c tr)n l/i !0i nhau ! raa i lVn& mli lVn !0i i` ml n0c ct trong $hGu chit A lo/i bd acetone. [hVn trn mkt c chuyAn !o bBnh nh mYc i` ml& nh mYc n !/ch b<ng nnhexane. Pau 6& dung dch ny +@c $ha lojng tF i n =^ lVn A $hHn t9ch hm lng carotenoid b<ng Muang $h2 E ! ho/t t9nh chOng oxy h6a li$o$hillic. Pa dWng ncarotene lm cht chu%n& o ) h$ thu ' b0c "6ng ]hu nm. =b59 c"'F5 c^c36C0D5D
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f. T"gc "$5" f.1 Vh0 .2G' #$ E59 c 0"! 59"2G4 f.1.1 Vh0 .2G' }c t@i& Cng& muOi& dVu Zn& yu trJng u` ) tr' ln& n$ ngon. ~te $etrol& xatri "ulcat Ehan& nnhexan& acetone& Dali bicroamat. f.1.) i59 c 0"! 59"2G4 rercher& buret& b$ i n& my X& "oxhlet. f.) T215 "$5" 0"! 59"2G4 f.).1 T215 "$5" 5e' @j2 9ec

Ko "/ch n$& ngHm n0c Eho#ng u giC. y.i 2 !o r cho ro n0c& tr)n thm i mulng c $h muOi qy $hVn n/c gc ring& ch !o `^cc ru trJng& dpng tay b6$ cho ru !0i gc nhuyGn !o nhau& lNc bd nh4ng m "i di Ehng tan ra. 5r)n $hVn n/c ! h)t gc !o n$ ! #o cho th,t fu Cho n$ j tr)n gc !o xang h$ ln cho ch9n& trong Mu trBnh . xi chFng m@i $hSt $h#i #o n$ m)t lVn cho xi ch9n fu. 5hCng b' "ung Cng& dVu Zn A tZng thm ) c#m Muan "au Ehi xi j ch9n f.).) T215 "$5" 0"! 59"2G4 @?c A5" "$4 .=B59 c36C0D5C2E ck 06C59 9ec 0=+2 #$ @j2 9ec 0"DC >"=+59 >"?> c"'F5 d. 5in hnh th9 nghi m = yu tO& i lVn lk$ l/i. u tO thay 2i l mRu em i xc nh hm lng carotenoid. u tO cO nh l Muy trBnh xc nh carotenoid theo $h@ng $h$ chu%n ). f.).f T215 "$5" 0"! 59"2G4 @?c A5" "$4 .=B59 c36C0D5C2E ck 06C59 9ec 0=+2 #$ @j2 9ec 0"DC >"=+59 >"?> V'359 >"a. 5in hnh th9 nghi m = yu tO& i lVn lk$ l/i. u tO thay 2i l mRu em i xc nh hm lng carotenoid. u tO cO nh l Muy trBnh xc nh carotenoid theo $h@ng $h$ chu%n ). *&' c(' P?C c?C 0"! 59"2G4 =. 5rBnh by c Muy trBnh ch bin xi gc& Muy trBnh xc nh hm lng carotenoid b<ng i $h@ng $h$. i. Po "ch hi u Mu# c(a i $h@ng $h$ Ehi "a dWng cho gc t@i ! xi gc Tc6 b#ng -no!a "o "nhU. u. Po "nh cht lng c(a xi gc Oi !0i gc t@i ban Vu Tc6 b#ng -no!a "o "nhU.

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