Neuronal Bursting Models in vivo and in silico

Lars Crawford Thida Myint

Recording in Leech Ganglion

Intrinsic burster Likely to be part of central pattern generator circuits Possibly a heart interneuron*

*Angstadt, JD, and RL Calabrese. "Calcium Currents and Graded Synaptic Transmission between Heart Interneurons of the Leech." The Journal of Neuroscience 11.3 (1991): 746-59. The Society for Neuroscience, 2013. Web. 19 Feb. 2013. <http://www.jneurosci.org/content/11/3/746>

Intrinsic Burster

Determining source of bursting

T-type Ca2+ channel
Activates at fairly negative thresholds Inactivates quickly, but incompletely Inactivation accumulates

Ca2+ dependent K+ channel

Stops constant frequency of spikes with increased [Ca2+]

Anode-Break Excitation

Discovering T-Ca2+ Threshold

Bursting at T-Ca2+ Threshold

Calcium Current in Response 1 nA Current Injection

-25 0 Membrane Voltage (mV) -27 50 100 150 200 250 300

-29
-31 -33 -35 -37 -39 y = 1E-10x5 - 9E-08x4 + 3E-05x3 - 0.0051x2 + 0.3615x - 37.438 R = 0.9868 Time (ms)

Voltage (mV)

Poly. (Voltage (mV))

Standard Hodgkin-Huxley Gating Variables

= 4 = 3

Standard Hodgkin-Huxley Model

Matlab Code for Bursting

= 2

Shorten, Paul and David Wall, "A Hodgkinhuxley Model Exhibiting Bursting Oscillations." Bulletin of Mathematical Biology. 62.4 (2000): 695-715. Print.

Bursting Model Based on HodgkinHuxley Equations

Optimal VCa= 53 mV

Slightly More Negative VCa

VCa= 50 mV

Even More Negative VCa

VCa= 40 mV

Bursting Goes Away (Vca<40 mV)

Definition of bursting:
Two or more action potentials per burst with an inter-burst period of at least 80 ms.