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  • Drugs and Behaviors

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    Godstruth
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    This document provides an overview of labeled immunoassays, which are used to detect antigens and antibodies that are too small or present at low concentrations to be detected by precipitation or agglutination tests. It describes different types of labeled immunoassays including radioimmunoassays which use radioactive labels, enzyme immunoassays which use enzymatic labels, fluorescent immunoassays which use fluorescent labels, and chemiluminescent immunoassays which detect light energy from chemical reactions. Key elements, methods, and techniques are discussed for each type of labeled immunoassay.

    Исходное описание:

    Actions and reactions of drugs.

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    This document provides an overview of labeled immunoassays, which are used to detect antigens and antibodies that are too small or present at low concentrations to be detected by precipitation or agglutination tests. It describes different types of labeled immunoassays including radioimmunoassays which use radioactive labels, enzyme immunoassays which use enzymatic labels, fluorescent immunoassays which use fluorescent labels, and chemiluminescent immunoassays which detect light energy from chemical reactions. Key elements, methods, and techniques are discussed for each type of labeled immunoassay.

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    12 просмотров13 страниц

    Drugs and Behaviors

    Загружено:

    Godstruth
    This document provides an overview of labeled immunoassays, which are used to detect antigens and antibodies that are too small or present at low concentrations to be detected by precipitation or agglutination tests. It describes different types of labeled immunoassays including radioimmunoassays which use radioactive labels, enzyme immunoassays which use enzymatic labels, fluorescent immunoassays which use fluorescent labels, and chemiluminescent immunoassays which detect light energy from chemical reactions. Key elements, methods, and techniques are discussed for each type of labeled immunoassay.

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    © All Rights Reserved
    Скачайте в формате PPT, PDF, TXT или читайте онлайн в Scribd
    Отметить как неприемлемый контент
    из 13

    CLS 1113 Introduction to Clinical Laboratory Practices

    Unit 5 Labeled Immunoassays Chapter 10

    Labeled Immunoassays
    Designed for Ags and Abs that DO NOT react in precipitation or agglutination tests due to their small size or low concentrations.
    Indirect method of detection:

    Competitive vs. Non-Competitive


    Test Antigen or Antibody competes for binding sites

    Elements of Labeled Immunoassays


    Ligands
    Antibodies Standards or Calibrators Separation Methods Detection of Label

    Radioimmunoassay (RIA)
    Competitive binding assay

    Uses a radioactive substance as a label


    3H - Tritiated hydrogen 125I - Iodine 125

    Radioimmunoassay
    Two Types
    Number 1

    Radioimmunoassay
    Number 2

    Enzyme Immunoassay
    Immunoassay labels
    Enzymes
    Cheap Readily available Long shelf life Easily adapted to automation

    ELISA, Figure 10-4, page 149

    Enzyme Immunoassay
    Enzymes are naturally occurring molecules that catalyze specific biochemical reactions.
    They react with suitable substances to produce products that are chromogenic (color), fluorogenic, or luminescent.

    ELISA: Sandwich method

    Figure 10-5, page 149

    Fluorescent Immunoassay
    Similar to ELISA but a fluorochrome is used rather than an enzyme.
    Fluorochromes have the ability to absorb energy from light an emit it at a longer wavelength.

    Fluorescent Immunoassay: Direct and Indirect

    Chemiluminescent Immunoassays
    Chemiluminescence is the production of light energy due to a chemical reaction.
    Certain substances when oxidized can give off short or long bursts of light energy.

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