Assignment 3 -Siddhant Sethi Q. What kind of peaks can we observe in HNCA? A.

In HNCA, each signal represents a single amino acid. At the frequency of each amide proton there are two cross signals in the Calpha dimension: One from the intraresidual and one from the interresidual Calphs atom. Using these cross signals a chain of correlations through the whole amino acid sequence can be established. However, proline residues interrupt that chain due to their missing amide proton.