0 оценок0% нашли этот документ полезным (0 голосов)
14 просмотров2 страницы
Two widely used terms in absorption spectrometry are: i)transmittance ii) absorbance. These terms are related to the ratio of power of incident beam of radiation(P o) and transmitted beam of radiation (P). Beer's Law (beer-lambert's law): for a monochromatic radiation, absorbance is directly proportional to the path length(b) through the concentration (c) of the absorbing species.
Two widely used terms in absorption spectrometry are: i)transmittance ii) absorbance. These terms are related to the ratio of power of incident beam of radiation(P o) and transmitted beam of radiation (P). Beer's Law (beer-lambert's law): for a monochromatic radiation, absorbance is directly proportional to the path length(b) through the concentration (c) of the absorbing species.
Two widely used terms in absorption spectrometry are: i)transmittance ii) absorbance. These terms are related to the ratio of power of incident beam of radiation(P o) and transmitted beam of radiation (P). Beer's Law (beer-lambert's law): for a monochromatic radiation, absorbance is directly proportional to the path length(b) through the concentration (c) of the absorbing species.
Two widely used terms in absorption spectrometry are:
i)Transmittance ii)Absorbance -These terms are related to the ratio of power of incident beam of radiation(P o ) & transmitted beam of radiation(P). i)Transmittance(T): -A beam of parellel radiation having power of P o is passed through a medium having a thickness of b cm & a concentration c of an absorbing species. -Interaction takes place between the photons & absorbing atoms or molecules. -As a consequence, the power beam is weaken from P o to P -Hence , the transmittance(T) is the fraction of radiation transmitted by the medium: T= P/ P o Expression of transmittance in percentage: %T = P/P o X 100%
ii)Absorbance(A):
A = -log 10 T = log log P o /P
-With the decrease in transmittance, absorbance of the medium increases. Beers Law (Beer-Lamberts Law):
For a monochromatic radiation, absorbance is directly proportional to the path length(b) through the concentration (c) of the absorbing species.
A= abc -------(i)
Where, a is a proportionality constant called absorptivity. its magnitude depends upon the units used for b & c. -When c is in moles per liter & b is in centimeters, then then the absorptivity(a) is called molar absorptivity ( , epsilon)
-Then the equation i) becomes: A = bc ---------(ii) has a unit of L mol -1 cm -1 . -Beers law is the basis for quantitative analysis. -It is used in both atomic & molecular absorption measurements.
Macromolecular Microsymposium — 16: Main Lectures Presented at the Sixteenth Microsymposium on Macromolecules (Advances in Scattering Methods), Prague, 12 - 16 July 1976