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(III)

2014 . 1, . 1, . -2, . 1, . T2,


. 1
1 , . , . ,
2 , . , .
3 3 , .
E-mail: bourosh.xray@phys.asm.md

[CoCl(DmgH)2H2O] (DmgH = )
(),
[Co(DH)2(N3)2]- : {[Na2(-H2O)2L2(H2O)2][CoIII(DmgH)2(N3)2]}+
[CoIII(DmgH)2(N3)2]- (L=(H2N)2C=NN) (I) [H3N-C(=NH)-NH-NH2]+
[Co(DmgH)2(N3)2]- (II).
, : (H3)
(2980-2930 -1); (H3) (1480 - 1360 -1); (N) (2065-2022-1); (NO-) (~1230 ~1084 -1)
~ 1750 -1,

[Co(DH)2(N3)2]-. I II (NH2) (NH)
3400-3200 -1, (NH2) 1650-1590 -1, (N) (I) 2024 -1.

, I
[CoIII(DmgH)2(N3)2]-, , N3-
Na [Na2(-H2O)2L2(H2O)2]2+ {[Na2(-H2O)2L2(H2O)2][CoIII(DmgH)2(N3)2]}+, Na
. Na 6
ON3 , L N3- [CoIII(DmgH)2(N3)2]-.
II ,
. I II NH2 NH
, I,
, N3-,
DmgH-.
The increased interest in obtaining and studying the coordination complexes of transition metals,
in many ways, is due to their biological properties, including the possibility of their use as regulators
and stimulants of biosynthesis of the microorganisms - producers of valuable biologically active
substances - enzymes, vitamins, antibiotics, lipids etc.

The obtained coordination compounds were tested in the possibility to stimulate the enzymatic
activity of Fusarium gibbosum CNMN FD -12 micromycete strain producer of acid, neutral and
alkaline proteases, about the possibility to use these hydrolases in biotechnological production.
The results shown that during 6 days of micromycete Fusarium gibbosum submerged cultivation,
with the supplementation of coordination compound I in optimally chosen concentration of 5-10 mg/L,
the acid proteases biosynthesis increased by 40-50%. This can ensure the obtaining of the proteolytic
enzyme preparation enriched with acid proteases.

H2NNHC(=S/Se)NH2 [14].
() [5] ,
,
,
, .. Ni(II)


H2L, (I). 0,65 NaN3 30 0,34
[Co(DH)2H2OCl] . 0,84 (CN 6h)
15 . H2L , ,
3--3--2
3
. 65%.
164165.
: 3673., 3415., 3242., 3196., 3154., 2971.., 2901., 1599 .., 1506.., 1471.,
1453., 1428., 1375., 1285., 1250., 1241., 1231., 1175., 1150., 1080., 1066.,
1056., 1047., 1038.., 1021., 990., 955., 938., 900., 854., 838., 748., 725.,
711., 696.
, %:

37.72; 7.36; N 29.25.

C6H14N4O1S1
; %: 37.87; 7.41; N 29.45.
[Co(HL)2]Cl2H2O (II). 0.24 (0.001 ) CoCl26H2O 10
0.38 (0.001) H2L, 10 .
5560 ~ 15 . -

, .
. 0.36
(65 %).
: 3416., 3256., 3160.., 2982., 2913., 2833., 1619., 1604., 1568., 1496..,
1467., 1442., 1393.,1365., 1339.., 1290., 1201., 1162., 1124., 1072., 1046.,
981., 952., 848., 784., 717., 688., 676., 655.
, %:

Co 11.78; C 28.20; H 5.87 N 22.00.

C12H30Cl1C1N8O4S2
; %: Co 11.58; C 28.32; H 5.94; N 22.02.
,

( 4 2013 .)

:
1. [Co(N3)(DH)2](NC=GU)

2. [Co(N3)(DH)2]()].



,
.

(pH 3,6), (pH 7,4) (pH 9,0)
Fusarium gibbosum CNMN FD 12.
,

(,
)
,
.
5 :
1,26 / ;
3,61 /
3,28 / .

, , 6 .
(. 510 /) 1 3,02 3,15 /, 2 1,34 /
1,26 / .

,
(5 )
1 [Co(N3)
(DH)2](NC=GU) 140 -150%. ,
15 /
.

-.

, 5
. , . 5 -10 / 3,36
/ 3,27 ,, 3,44 / 3,61 / 3,28
/, .
2 4
2,85 / (. 10 /),
(3,61 / 5 ) 26,7 %.

5 2,94 / (. 10 /),
(3,28 /) 11,6 %. 6

1,47 /, -
2,18 0,5 /,
5 / 15 /; 2
2,60 /.

, (/):
20,0

10,0
CaCO3
2,0
(NH4)2SO4
1,0
H2O
1 ,
pH 6,25 t 28-30 ,
180 200 /.
4-6 ,
.
5, 10, 15 /.
.
,
Fusarium gibbosum 6 1
5 10 /,
140 150 %,
, .

1. ( , )
Fusarium gibbosum CNMN FD 12 (/)

.
/
5

[Co(N3)(DH)2](NC=GU)

10
15
5

[Co(N3)(DH)2]()]
.

10
15

(pH 3,6)

(pH 7,4)

(pH 9,0)

4 c.

5c.

6 c.

4 c.

5c.

6 c.

4 c.

5c.

6 c.

0.50

0.84

3.02

2.10

3.36

0.00

1.68

3.44

2.18

0.75

3.15

2.10

3.27

0.00

1.68

3.44

1.59

0.25

1.43

1.90

2.35

0.04

1.43

2.43

0.59

0.55

1.34

2.52

0.34

0.00

1.26

1.43

0.00

0.61

1.34

2.85

0.67

0.00

2.68

2.94

0.08

0.52

1.26

2.18

0.84

0.00

2.26

0.92

0.00

1.26

0.42

3.40

3.61

1.47

2.61

3.28

2.60

0.50
0.25
0.38
0.34
0.42
0.65