SPERM PREPARATION TECHNIQUES FOR

ART WITH BRIEF REFERENCE TO THE

WHO SPECIFIED QUALITY CONTROL
PROTOCOLS

BY
ARITRI GHOSH

SUPERVISED BY
DR. S.GHOSH DASTIDAR

GHOSH DASTIDAR INSTITUTE OF INFERTILITY AND RESEARCH

208, RASHBEHARI AVENUE, KOLKATA-700069
ACKNOWLEDGEMENTS
 CONTENTS

SUBJECT PAGE

 GENERAL INTRODUCTION:

 INTRODUCTION

 SPERMATOGENESIS

 SPERM MORPHOLOGY

 DIFFERENT TECHNIQUES OF SPERM PREPARATION:

 SPERM ASSESSMENT:

EOSIN-NIGROSIN TEST

HOS TEST

 SEMEN WASH TECHNIQUES:

DIRECT WASH

SWIM UP TECHNIQUE

SWIM DOWN TECHNIQUE/DENSITY GRADIENT TECHNIQUE

 QUALITY CONTROL WITH RESPECT TO WHO PROTOCOLS:

 RESULTS AND DISCUSSION:

 REFERENCE AND BIBLIOGRAPHY: