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2009 /

ELISA
(IBV)
Dr. Bart van Leerdam, BioChek B.V. , Burg. Bracklaan 57, 2811 BP Reeuwijk, Netherlands
Dr Pieter Kuhne, Intervet/Schering Plough Animal Health, P.O. Box 31 5830 AA Boxmeer, Netherlands
, ELISA, ,
(IBV).
.

(IBV) ELISA. , :
(CV)
IBV?
IBV ELISA?. IBV
ELISA ,

www.intervet.ru

www.intervet.ru

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IBV

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IBV, .
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, (,
) IBV.

www.intervet.ru

www.intervet.ru

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ELISA:
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ELISA

ELISA , :
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.
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.
IBV
1 2 ( ). , ( ) . H120
,
, IB 4/91.
2. , %CV ( ). ? %CV , ?

www.intervet.ru

www.intervet.ru

:
%CV

40%

40-60%

60%

,
, IBV,
. , , , IBV
, ELISA. , H120, CV
40-70%. CV <30% H120
, . ,
, 4/91,
CV 45% .
-,

, (100% )
, %CV.
,

. , (100%) .
3. ,
.
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)

. , , (,
)
. , IBV

www.intervet.ru

www.intervet.ru

.
,
IBV ,
.
, ,
.
. , , , 4/91,
- .
,
H120 MA5,
IBV 4/91
10-15 , ,
IBV 18 .

24 , , 4/91.

BioChek :
ELISA 24 :
4/91

2000 5000

4/91 8

6000 16000

, ,

4/91 . 4-8 .



, ,

. 100% .

: .. , , .


. IBV -
. .
,

www.intervet.ru

www.intervet.ru

,
.
IBV,
. ,
IBV.

IBV

, , , .
.

IBV
IBV. IBV
( ).
ELISA : , .
, .. , .
, ,
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.
, , . ,
,
, .

IBV
IBV ,
, ,
IBV:
1) . ,
, ,
, ,
,
.
2) CV .
(CV) ,
,

www.intervet.ru

www.intervet.ru

CV . ,
- H120, CV 50-80%.
CV 35%,
.
3)
. IBV , -

IBV, IBV.
IBV
- .
3 .

1. IBV (34 ) (37 ).


(CV)
. IBV .
35 15%
, 34

, 37
# samples

# samples
8
7
6
5
4
3
2
1
0

Mean Titer:
%CV:

10

3922
58

Mean Titer:
%CV:

15498
28

6
4
2
0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

Titer group

Titer group

: , 15 %
%CV
2. 44 , H120. ,
(CV) . .
IBV
1- 120 19 120 .
28- . 44 .
# samples
6
5
4
3
2
1
0

Assay:
Lot:
Bleeding Date:
Testing Date:

IBV
FS4063
03/02/2005
04/02/2005

Mean Titer:
G.M.T.:
%CV:

16704
15637
33

Expected Vac. Titer:


Expected % CV range:

1000  2000
40  60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

Titer group
Details Vaccination Program:
Vaccine
Method
H120
M. spraying
H120
M. drinking

Age Vacc.Batch/Applicator
OID
19D/

IBV
,
IBV,

:

www.intervet.ru

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1) IBV , ,
(ART) ( ,
(APV)), Ornithobacterium rhinotracheale (OR),
Mycoplasma gallisepticum (MG) Mycoplasma synoviae
(MS).
. -

www.intervet.ru

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IBV
.
2) IBV
NDV - ,
( / ).
,

IBV NDV.
(ART), IBV, IBV.

,
. ( ) :

/ (%CV).

.
1
,

(IBV)

IBV MA5.

21
10-15%. , E. coli.
ELISA
, . ,
, , -
E. coli.
IBV,
. , ART OR (. 1).
, ,
,
. (ART) (OR),
,
ART ( )
OR ( ).
IBV ,
( 2
). (CV) ,
,
, ART OR.
,
ART 7 ,
.

1
# samples
3.2
2.8
2.4
2
1.6
1.2
0.8
0.4
0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

Titer group

# samples
2
1.6
1.2
0.8
0.4
0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

Titer group

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Assay:
Lot:
Bleeding Date:
Testing Date:

O.r.
FS3784
05/11/2002
11/11/2003

Mean Titer:
Min.Max Titer:
G.M.T.:
%CV:

6957
1235  23286
4823
93

Assay:
Bleeding Date:
Testing Date:

ART
05/11/2002
11/11/2003

Mean Titer:
Min.Max Titer:
G.M.T.:
%CV:
Target Titer:
Target % CV:
Interpretation titer:
Interpretation CV:

11373
2311  20033
9728
50
0  1600
50  200
HIGH
NORMAL

www.intervet.ru

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2
IBV


Massachusetts (H120) 1 20 .
NDV
Avinew 1 () 20 (
). 14-21 , . .
. . (
1), () ( 2)
( 3).

, ART OR .
,
IBV (4/91) 14 .
.
,
ELISA, Intervet , ,
(VN).
:
2
IBV
QX-

- H120
1 20 .
14-21 , .
46 .

(25 )

ELISA
46 . MG MS
( ). IBV
CV (25%), (50
100%).
IBV.
ART OR , ,
.
NDV
, , ,

. NDV
, . (
). ,

www.intervet.ru

www.intervet.ru

46 ,

46 ,

ELISA

500 - 2 000

4 446

BioChek

CV 50-100%

CV 25%

VN M41

>7,0

8,4

VN 4/91

<7,0

8,1

VN QX (D338)

<7,0

9,6

(VN)
QX- ( D388) ,
120 Massachusetts ( VN
M41). , ELISA

( IBV) ,
IB 4/91.

(VN)
( D338).
.

.

www.intervet.ru

www.intervet.ru

10

# samples

8
6
4
2
0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

Titer group
Details Vaccination Program:
Vaccine
Method
Bioral H120
Coarse Spray
Bioral H120
Dr. Water

10

6
4
2
0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

Titer group

14
12
10
8
6
4
2
0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

# samples

10
8
6
4
2
0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

Titer group
Details Vaccination Program:
Vaccine
Method
Avinew
Coarse Spray
Avinew
Dr. Water

www.intervet.ru

Mean Titer:
Min.Max Titer:
G.M.T.:
%CV:
Target Titer:
Target % CV:
Interpretation titer:
Interpretation CV:

4466
1776  6824
4289
25
500  2000
50  100
HIGH
LOW

Assay:
LOT:
Bleeding Date:
Testing Date:

ART
FS4723
06/02/2008
07/02/2008

Mean Titer:
Min.Max Titer:
G.M.T.:
%CV:
Target Titer:
Target % CV:
Interpretation titer:
Interpretation CV:

2713
851  5083
2476
42
0  1600
50  200
HIGH
LOW

Assay:
LOT:
Bleeding Date:
Testing Date:

O.r.
FS4701
06/02/2008
07/02/2008

Mean Titer:
Min.Max Titer:
G.M.T.:
%CV:

3187
298  26532
1671
166

Assay:
LOT:
Bleeding Date:
Testing Date:

NDV
FS4708
06/02/2008
07/02/2008

Mean Titer:
Min.Max Titer:
G.M.T.:
%CV:
Target Titer:
Target % CV:
Interpretation titer:
Interpretation CV:

6778
2967  11162
6459
30
1000  4000
40  70
HIGH
LOW

# samples

Titer group

12

IBV
FS4712
06/02/2008
07/02/2008

Age Vacc.Batch/Applicator
OID
20D/

# samples

Assay:
LOT:
Bleeding Date:
Testing Date:

Age Vacc.Batch/Applicator
OID
20D/

www.intervet.ru

www.intervet.ru

www.intervet.ru



ELISA
:



,
.

. ,
IBV . .
.

ELISA

, IBV. , . :
,
ELISA.
IBV .
ELISA

.
IBV, .
ELISA
.

.
, , .

1. BIOCHECK:
, ,
. .

IBV


(35 40 .)

, 1

(H120)

300 1 500

> 3 000

, 1

(MA5, IB Primer)

1000 2 000

> 4 000

, 2

(H120)

1000 2000

> 4 000

, 2

(MA5, IB Primer)

2000 4 000

> 6 000

, 2

(H120 + 4/91)

3 000 6 000

> 9 000

.
BioChek , .
2. BIOCHECK: -/
, ,
. .

IBV

(H120)

1000 2 000

3 5

> 4 000

(MA5)

1 000 4 000

3 5

> 6 000

(l- H120,
2- 4/91)

6 000 10 000

3 5

> 12 000

6 000 17 000

5 8


16-18 .
.
BioChek , .
125445, , , . , . 24 ,
. (495) 956 7140/44, (495) 956 7141/45. www.intervet.ru