6 Lacsamana Lanuza Latonio Lee March 11, 2014

Pink
K. oxytoca
Gram-negative
cocci-bacilli
MICRO 200L WORKSHEET FOR BIOCHEMICAL TESTS
Group # ______ Surnames of members ___________/___________/___________/___________ Date ______________ Score ________
Name of
medium
Name of test Color of
medium
Appearance of
Positive result
Appearance of
Negative
result
Results for known and unknown organisms (+/-)
S. aureus M. luteus B. subtilis E. coli E. aerogenes K. oxytoca Unknown
MSA Test for
mannitol
fermenters
Bloody
mary
Yellow
colonies
Colorless
colonies
+ - No growth + + - -
MCA Test for lactose
fermenters
Red
Red or intense
pink colonies
Cream-colored
or colorless
No growth No growth No growth + + + +
EMB Test for lactose
fermenters
Red Greenish
metallic sheen
No greenish
metallic sheen
- - - + - - -
TSIA Test for sugar
util. & H2S
Red-
orange
Red color Yellow color
A/K K/A K/A + + + gas prod. K/A K/A
Tryptone
broth
indole
production test
Yellow
Red ring on the
surface
No red ring
on surface
- - + + - + +
NB+G (MR)
production of
acids and
(AMC) test
yellow Red color Yellow color
- - + - - + +
NB+G (VP) Yellow Pink to red color No change in
color
- - - - - + +
Simmons
citrate
Citrate
utilization test
Green
Blue color and
growth
No color change
- - - - + + +
Starch agar Champagne Colorless Colorless zone
surrounding
colonies
Formation of
blue-black
complex
- - + - - - -
LIA Purple Violet
Lysine decarboxylation=
purple slant, purple butt
Lysine deamination= red
slant yellowbutt
H2S production-
blackening
Purple slant
Yellow butt
+ decarbox. - - + + +
decarbox.
+
decarbox.
OF
medium
Oxidation-
fermentation
test
Green
Oxidation, w/o oil-
yellow w/oil-no
change
Alkaline: blue for both
Fermentation: both
yellow
No reaction/ No
color change
No reaction Oxidation No growth Ferment. + ferment. + ferment. + ferment.
Catalase test Colorless Bubble
formation
No bubbles
+ + + + + + +
Ng Gelatin
hydrolysis
Light
yellow
Fluid Gelatin
- - - - - - -

Probable identity of given Unknown: _______________
Gram stain of Unknown: _________________________
Gram reaction: _________________________________
Cell shape: ____________________________________