membrane against the concentration gradient, that is from a region of low concentration to a region of high concentration. Active transport also requires specific carrier proteins that bind to the substance to be transported, enabling the substance to pass rapidly across the plasma membrane. Sometimes the carrier proteins are referred to biological pumps. Active transport requires an input of energy provided by ATP. So, it can only take place in living organism. Sodium-potassium pump Ion intake by root hairs of a plant Mineral ions are absorbed across the membranes of the root hairs by active transport.