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SarahLockhart

EXTRACTIONLABWRITEUP
I.ABSTRACT
Forthislab,wetookourownsalivasamplesandusedsaltwater,detergent,isopropylalcohol,
meattenderizertoextractamacromolecule.Wefirstgargledsaltwatersolutionandspititintoa
plasticcup.Thenweaddedadropofdetergentandapinchofmeattenderizerandcarefully
stirred.Thenwepouredathinlayerofisopropylalcoholandsawacouplethin,whitestrings
appear,whichwepredictedwerenucleicacids.

II.FINALMIXTURE
MygroupandIsawtwothin,whitestringsinthesolution.Wethoughttheywerenucleicacids
becauseDNAandRNAcanbefoundinsaliva.Wealsothoughtthatmeattenderizerwould
probablybreakdowntheproteins,lipids,andcarbohydratesthatmightalsobefoundinsaliva.I
thinkitisimportanttoisolatenucleicacidsbecausetheycontainagreatamountofgenetic
information.

III.MATERIALS
Thesaltwatersolutionprovidesaconvenientvesseltocollectthesalivawithandthesalthelps
theDNAsticktogetherafteritisextractedandmakesitmorevisibletothenakedeye.Adding
thedetergentbreaksdownthecellmembranebyremovingthelipidsandproteins,sowecan
getthenucleicacidsoutofthenucleusofthecell.Themeattenderizerisanenzymethat
removestheproteinssurroundingtheDNAinthenucleus.Isopropylalcoholisaddedontop
becauseDNAusuallydissolvesinwater,butitclumpstogetherandrisesintothealcohol,
makingitvisible.

IV.THEMACROMOLECULE
ThemacromoleculeweextractedwasDNA,whichisanucleicacidthatisstoredinsidethe
nucleus.FromthislabIlearnedthatcellsareveryresponsivetotheirsurroundingsandcommon
householditemscanbeusedtomanipulatethem.Butitalsoshowedmethatnucleicacidsare
veryresilientandarehardertobreakdownthanothermacromoleculeslikelipidsandproteins
andcarbohydrates.

V.IMPROVINGOUTCOME
Ithinkmygroupwouldhaveisolatedmorenucleicacidsifwehaddonetheprocedureproperly
thefirsttime.Iwasthegroupmemberthesalivasamplewastakenfromandweendedup
havingtotrythreetimestogetitright,somymouthwasmostlydampfromtheothersaltwater
trials,notactualsaliva.Ialsothinkthatmanygroupsdidnotusetheproperamountofeach
material,soifeveryonewentthroughtheprocessslowerandwithmorecarewewouldhave
morepreciseresults.
VI.RESOURCES
Myownnotesfromclass
HowtoExtractDNA,GeneticScienceLearningCenter:UniversityofUtahHealth
Sciences

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