.

639.3/.6
1

.. 2, .. 2, .. 1,2
,
690087, . , . , 52
2
. .. , 690041,
. , . , 17

.
( , )
. . . , : , ; , - ;
; Skeletonema costatum . . ,
.
.
: , , , , , .

.. Begun, S.I. Maslennikov, A.Yu. Zvyagintsev

PHYTOPLANKTON IN TREATMENT FACILITIES AREA NEAR VLADIVOSTOK
(AMURSKII BAY, JAPAN SEA)
First time the species composition and quantitative parameters dynamics of phytoplankton in northern of Amurskii Bay in treatment facilities area near Vladivostok are studied. The following peculiarities
in the phytoplankton quantitative development is a typical to anthropogenic eutrophication waters were
revealed: increase number of bloom species; increase general phytoplankton density appropriate to extremel-eutrophic type in local mounts; increase density of flagellates phytoplankton components relatively
diatoms; Skeletonema costatum diatom bloom and connection to them decrease Shannon and Pielou indexes. The results of obtain investigation shown presence of anthropogenic eutrophication waters in
treatment facilities area near Vladivostok that generally caused by uncontrolled water escape including
mineral and organic dissolved elements together underpurified waters in northern area of Amurskii Bay.
Key words: phytoplankton, Amurskii Bay, treatment facilities, diatoms, eutrophication, bloom.

, - . .
, 3

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. . -
. [5, 10, 11]. 20 1990- . :
, - , [9].

. , , , ,
.
.
90- . [15, 16, 20].
. - -,
. , .
.
. .


. . 2002 2003 .
1-2 . . ,
- 2 .
0,05
0,07 , 1 .
1 [4]. () Jenamed 2, Olympus BX41, UPLanF1 100/1/.30, .
Pseudo-nitzschia
() JEM 100.
, .
() [21], , (25, 50, 75, 96 100 %) . Edwards, AUTO 306
.
Leo-430 500-5000 . (),
().
4

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

83 : Cyanophyta (2 ), Chrysophyta (1), Bacillariophyta (46),
Cryptophyta (2), Dinophyta (27), Euglenophyta (2), Raphidophyta (1) Chlorophyta (2).

Chaetoceros, Eucampia, Melosira, Leptocylindrus, Skeletonema, Thalassionema,
Protoperidinium, Dinophysis Dictyocha. (75,9 %), ,
, 3,6 %. Pleurosigma naviculaceum, Grammatophora
marina Licmophora abbreviata. (10,8 %), ,
, Melosira moniliformis, Odontella aurita
Cylindrotheca closterium.

. 1. -
. ( ., ) 2002-2003 .:
1-2 Nitzschia frigida Grun.; 3-4 Navicula vanhoeffenii Gran; 5-6 Detonula confervacea (Cl.) Gran
Fig. 1. Ice and neritic diatoms dominants of winter phytoplankton in treatment facilities area near
Vladivostok (Amurskii Bay, Japan Sea) in 2002-2003: 1-2 Nitzschia frigida Grun.;
3-4 Navicula vanhoeffenii Gran; 5-6 Detonula confervacea (Cl.) Gran

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

(49,9 %)
(40,9 %), . (69,9 %), - (8,4 %), (3,6 %), - ( 1, 2 % ).

, .
28,9 % ,
-- (14,4 %), (12 %) - (10,8) (4,8 %), (2,4 %)
(1,2 %).
10,6 .
15 ./, 0,07 51,7 /3. : , , , (. 2, ) - : , (. 2, ).
(1,13 ./) ,
Skeletonema costatum Dactyliosolen fragilissimus. (1,3-4,6 ./) Detonula confervacea Thalassiosira nordenskioeldii, Chaetoceros pseudocrinitus, Navicula
vanhoetfenii Nitzschia frigida (. 1, 1-6)
(-1,8 ) (. 2, ). (15 ./)
14 (. 2, ),
Chlamydomonas sp.
(11,4 ./)
S. costatum. (966 . ./)
S. costatum
Plagioselmis prolonga
C. globosa.
(5 /3) D. fragilissimus P. pungens (. 2, ).
(9,2-51,7 /3), , , T. nordenskioeldii D. onfervacea.
.
, , - . ,
, , .. ,
[8]. , , , .
( ). , , , , [19].
6

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. 2.
. ( ., ) 2002-2003 .:
;
; ;
; (H)
(e) ;
Fig. 2. Quantitative parameters phytoplankton dynamic in treatment facilities area near Vladivostok
(Amurskii Bay, Japan Sea) in 2002-2003: seasonal dynamic of absolutely phytoplankton density;
seasonal dynamic of relatively phytoplankton density; seasonal dynamic of absolutely
phytoplankton biomass; seasonal dynamic of relatively phytoplankton biomass, seasonal
dynamic of Shannon and Pielou indexes; seasonal dynamic of temperature and salinity water

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

D. confervacea, N. vanhoetfenii N. frigida

, .
, -
, ,
, [13].
,
81,5 %
77,6 % (. . 2, , ). 98-100 %
, ,
68 %. , S. costatum (85,1 %).
1,23 %
11,6 % (. . 2, , ). - (70-96 %).
(0-1,2 %
0-17,5 % ). , (14 %). (2,79 % 22,8 % ). (99 98 % ). (77,3
47,1 % ) (77,5 2,44 % ).
0,1 % .
, , ,
, () . .
, . - -, , , [11]. , , , ,
[14].
( 15 ./), . (1,92 ./). 1991-1993 . [6] , ., .
(17,9 ./),
(5,3 ./).
,
S. costatum

. . 8

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

, , -, , [18].
S. costatum - . ,
[3].
8 (S. costatum, T. nordenskioeldii, C. pseudocrinitus,
D. confervacea, H. triqetra, E. gimnastica, Plagioselmis sp., Chlamydomonas sp.).

(15 ./ Chlamydomonas sp.),
(4,4 ./ D. confervacea) (3,6 ./ S. costatum) - . , ,
. [6]. .
., - [11].
, .,
, ,
, [14].
, , , , , . - .
,
,
.
( ., ) 2002-2003 .
Total density and biomass parameters of phytoplankton, Shannon and Pielou indexes,
density and biomass of diatoms and flagellates phytoplankton components in treatment
facilities area near Vladivostok (Amurskii Bay, Japan Sea) in 2002-2003
,
./

0,001
1,7
15,0
()

0,004
1,1
2,39
, ./

0,084
0,65
3,59
3
, /

0,0027
8,65
51,8

,
/3

0,007
8,9
51,7
()

0,001
0,4
0,87
, ./

0,0006
1,05
15
3
, /

0,0016
0,22
2,46

, ,
, , (. ). 9

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

, . .
36- , -
[15, 20] 44- ,
[2].

. [15, 20]
.
S. costatum.

,
[12]. ,
,
[17].
, . ,
. , ; , - ;
; S. costatum . , , - D. confervacea,
N. vanhoetfenii N. frigida, .
.
,
. .
, , . , , ,
[1].
, .
.
,
10

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

,
. - , .
[7].

1. .. -
[] / .. , .. , .. . -: . Pilies
Studio, 2006. 498 .
2. .. . (
) [] / .. // . . 2004.
. 138. . 330-344.
3. ..
[] / .. , .. // .
. 2010. . 161. . 177-198.
4. .. : . [] / .. . , 1989. . 136-540.
5. .. [] / .. // . . 2000. . 26, 3. .
149-159.
6. .. [] / .. , .. , .. . .: , 1989. 167 .
7. .. . [] / .. , .. , .. , .. // . . 2003,
, . .. . :
, 2003. . 193-195.
8. .. [] / .. , .. // . 1987. . 2. . 317-321.
9. ..
( ) [] / .. // . .
2007. 1. . 7376.
10. .. -
[] / .. . : - , 2001. 193 .
11. : / . . . -. , 2002. . 1. . 1. 57 .
12. .. ()
[] / .. , .. // . 2001. . 11, 1. . 70-82.
13. [] / .. . .: , 1974. 239 .
14. - 1999 : /
; . . .. . -, 1999. 263 .
15. .. [] / .. , .. // . . 1995. . 21,
6. . 403-406.
11

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

16. .. -
[] / .. , .. // . . 1998. . 24, 4. . 205-211.
17. .. : . .
. . . , 2004. 21 .
18. .. - [] / .. , .. // . 1972. . 12. . 1. . 111-121.
19. Eide I., Martin S. The formation of brine drainage features in young sea ice // J. Glaciol. 1975. Vol. 14, . 70. P. 137-154.
20. Stonik I.V., Orlova T.Yu. Phytoplankton of the coastal waters off Vladivostok (the
North-western part of the East Sea) under Eutrophic Conditions // Ocean and Polar Research.
2002. Vol. 24, . 4. P. 359-365.
21. Truby E.W. Preparation of single-celled marine dinoflagellates for electron microscopy // Microscopy Research and Technique. 1997. Vol. 36. P. 377-340.
: , ,
, e-mail: andrejbegun@yandex.ru;
, , , e-mail: ayzvyagin@gmail.com;
, ,
, , e-mail: aqua@imb.dvo.ru.

12

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

639.3/6
.. 2, .. 1,2, .. 3
1
,
690087, . , . , 52
2
. .. ,
690041, . , . , 17
3
, . , . , 19

SARGASSUM PALLIDUM (TURNER) C. AGARDH
( , )
Sargassum pallidum 19
Bacillariophyceae (11), Fragilariophyceae (4)
Coscinodiscophyceae(4).
Tabularia fasciculata, Achnanthes brevipes, Licmophora abbreviata Melosira moniliformis , . Sargassum pallidum
, . -
-,
. , .
: , , , .

.. Begun, S.I. Maslennikov, A.B. Kruchkova

DIATOMS OF EPIPHYTON OF SARGASSUM PALLIDUM (TURNER)
C. AGARDT (PHAEOPHYTA) IN THE TICHAYA ZAVOD BAY
(VOSTOK BAY, JAPAN SEA)
In total, 19 species and intraspecific taxa of diatoms including Bacillariophyceae (11), Fragilariophyceae (4) Coscinodiscophyceae classes in epiphyton of Sargassum pallidum (Phaeophyta)
are revealed. The diatoms Tabularia fasciculata, Achnanthes brevipes, Licmophora abbreviata and
Melosira moniliformis typical for fouling of natural and artificial surfaces colonial attached algae
determining peculiarities of epiphyton quantitative abundance. The fouling of S. pallidum by diatoms
make it possible to consider as temperate level, that is character for macrophytes having axial type
thallus. The saprobiological analysis of diatoms has shown domination - and -mesosaprobiontes in
epiphyton, that evidences to temperate level of organic water pollution by caused generally natural
eutrophication in Tichaya Zavod Bay (Vostok Bay) in summer.
Key words: epiphyton, diatoms, saprobiontes, macrophytes.

-
. , ,
[2, 10]. - ,
13

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

-
,
[1].
[10, 12].

- [5, 8, 9, 11],
.
- . [6, 2]. - . .
Sargassum
pallidum (Turner) C. Agardh . .

S. pallidum ( , . ), 1-1,5 2010 .
.
Nitex (Sefar, ) 20 80 .
4-5 . Jenamed 2,
Olympus BX41, UPLanF1 100/1/.30 . [1].

S.
pallidum 19 Bacillariophyceae (11), Fragilariophyceae (4) Coscinodiscophyceae (4)
().
Pleurosigma W. Sm. (3).
Tabularia fasciculata
, . , Achnanthes brevipes Licmophora abbreviata Melosira moniliformis ,
(). (Berkeleya, Fragilaria,
Licmophora, Navicula, Parlibellus, Rhoicosphenia) , [16].
14

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

, -

Sargassum pallidum (Turner) C. Agardh . 2010 .
List of species, ecological and geographical characteristic and relatively quantitative
abundance of diatoms in epiphyton of Sargassum pallidum (Phaeophyta) in Vostok Bay
(Japan Sea) in august 2010

Achnanthes brevipes Agardh

Arahnodiscus ehrenbergii Bail.

Cocconeis scutellum Ehrenb.

-

Coscinodiscus oculus-iridis Ehrenb.

Donkinia recta (Donkin) Grunov

Gyrosigma tenuissimum (W. Sm.)

Griff. et Henfr.
Licmophora abbreviata Agardh

Melosira moniliformis (O.F. Mll.)

Agardh
Nitzschia longissima (Brb. ex Ktz.)

Ralfs ex Pritch.
Parlibellus rhombica (Greg.)

L.I. Ryab.
Pleurosigma formosum W. Sm.

P. intermedium W. Sm.

P. naviculaceum Brb.

Rhabdonema arcuatum (Lyngb.) Kutz.

Rhizosolenia setigera Brightw.

Rhoicosphenia marina (W. Sm.)

M. Schmidt
Tabularia fasciculata (Agardh)

Williams et Round
Trachyneis aspera (Ehrenb.) Cleve

Undatella quadrata (Brb. ex Ktz.)

Padd & Sims

. : , -; : , ; : , ; - -; : , -; : -, -; -
: , , , -, -;
[1]: , , , , .

15

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

Cocconeis scutellum, Gyrosigma

tenuissimum, Pleurosigma formosum Rhabdonema arcuatum Rhoicosphenia
marina. , . 6 (-) 2 (-) . - (8) - (5) . (12), , - (6),
, . ,
, , - [13]. S. pallidum Rhizosolenia setigera
. [15].
(19)
S. pallidum , ,
, [3, 9].
-, . [12].
Cladophora, Polysiphonia, Ceratium .
, .
Sargassum [6], Saccharina (Laminaria),
,
[11].
S. pallidum , , ,
, ,
. C. scutellum,
L. abbreviata T. fasciculat . ,
,
[2, 10]. - - .
[1] , . [7].
, S. pallidum .
. [3]. , , , ,
16

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

, . , .

Sargassum pallidum . : 1 Achnanthes brevipes;

2 Cocconeis scutellum; 3 Licmophora abbreviata; 4 Melosira moniliformis;
5 Rhabdonema arcuatum; 6 Pleurosigma naviculaceum; 7 Rhoicosphenia marina;
8-10 Tabularia fascuculata
Cells and colonial general view of mass epiphyton diatoms of Sargassum pallidum
(Phaeophytes) in Vostok Bay (Japan Sea): 1 Achnanthes brevipes; 2 Cocconeis scutellum;
3 Licmophora abbreviata; 4 Melosira moniliformis; 5 Rhabdonema arcuatum;
6 Pleurosigma naviculaceum; 7 Rhoicosphenia marina; 8-10 Tabularia fascuculata

. , . . 0,4, . . (5,9) (41,0) [7].

, . , - ,
- [9], [16]. , -
(), , [18].
17

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

( .),
[3].
,
[9].
, S. pallidum . - -,
2010 .,
- . - ,

.
. .
, , , ,
,
.
, , ,
[14]. , . , . .

.

1. .. -
[] / .. , .. , .. . -: . Pilies
Studio, 2006. 498 .
2. .. Phyllospadix iwatensis Makino [] / .. , .. //
: . . . VII
. --, 9-13 2008 . -/., 2008. . 44-46.
3. ..
( ) [] / .. , .. , .. //
. 2009. . 19, 3. . 257-272.
4. . . .: , 1974.
. I. 400 .
18

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

5. .. Cystoseira
barbata (Turn.) Ag. (Phaeophyta, Phycales) [] / .. // . .
1982. . 106. . 154-158.
6. ..
( , ) [] / .. , .. // . . 2008. . 154. . 248-258.
7. .. -
[] / .. . : - , 2001. 193 .
8. .. [] / .. //
. . 1975. . 98. . 193-198.
9. .. -
: . . ... . . . , 1986. 24 .
10. .. : . . - . . : , 2009. 44 .
11. .. ,
[] / .. , .. //
: . . 2- . . . (, ., 1982).
, 1982. . 3. . 91-92.
12. ..
[] / .. , .. // . . 1992. . 77. 5.
. 33-39.
13. ..
() [] / .. , .. , .. , .. // . 2004. . 14, 1. . 48-61.
14. .. i . [] / .. , .. //
. . . 1999. . 56, 3. . 266-275.
15. ..
[] / .., ..
, .. , .. // . 1999. T. 9, 3. . 32-42.
16. .. [] / .. , .. // . . 1995. . 21,
6. . 403-406.
17. Hoagland K.D., Rosowsk J.R., Gretz M.R., Roemer S.K. Diatom extracellular polymeric substances: function, fine structure, chemistry and physiology [Text] // J. Phycol.
1993. Vol. 29. P. 537-566.
18. Lewin J.C., Lewin R. Auxotrophy and heterotrophy in marine littoral diatoms [Text] //
Can. J. Microbiol. 1960. Vol. 6. P. 127-134.
: , ,
, e-mail: andrejbegun@yandex.ru;
, , ,
, e-mail: aqua@imb.dvo.ru;
, , e-mail: ankin88@mail.ru.

19

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

541.183.628515
.. , .. , ..

690087, . , . , 52

, ,
- , .
.
: , , ,
.

L.S. Byankina, N.A. Vaskova, I.G. Khalchenko

APPLICATION OF ELECTROCHEMICAL METHOD AND MODIFIED SORBENTS
FOR THE NEUTRALIZATION OF CHEMICAL POLLUTION OF WASTEWATER
FROM ELECTROPLATING PRODUCTION
In this paper we explored the possibility of water purification obtained after electrolytic
production of heavy metals by electroflotation with the use of ruthenium oxide-titanium anode, and
sea water quality in the space of additional coagulant, and the effect of modified sorbents to clean up
galvanic. Concluded that the adsorption method as the most-more promising for galvanic treatment of
heavy metals.
Keywords: wastewater, galvanic, heavy metals, electro-chemical adsorption and purification
methods.

.

( , , , ), ( ,
, - , ).
() , . , , , , , , , , , , .
,
.
0,46 . ,
3,3 . , . , , . ,
20

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

, , , ,
. ,
(0,001 /) , ( 0,004 /)
. , .
, , ,
, .
, -. ( ).

[1].
( ) .
( ), .
, , , , . , .

, , - ()
. , .
- =6. , 9, NaOH (10 /).
.
, 35 % , 0,5 .
. 30, 60,
90 . - 180-50.
. 1.
, , . 35 %
,
84 % (Ni) 99,6 % (Zn). 56,8 %. , , , .
21

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

1
Table 1

,
/

+ 35 %

Cd
Pb
Ni
Cr
Cu
Zn
Fe

12,0
0,37
0,91
8,00
1,14
5,61
0,30

0,68
0,16
0,15
0,52
0,01
0,02
0,04

94,3
56,8
83,5
93,5
99,1
99,6
86,7

30
60
90
4,00
0,15
0,14
0,94
0,08
0,24
0,08

4,00
0,14
0,17
0,73
0,31
0,71
0,28

6,00
0,13
0,14
1,18
0,245
2,07
0,72

50
64,86
84,6
85,25
78,5
63,1
-140

,
. Cd, Cr, Zn, Fe, ..
, .
, ,
: , , , , . , , ,
.
,
, : (
). , ( ) . ( ), c .
. , .
. , : 96 2/ ( ) 32 2/
.
.
. ,
, . , ,
.
,
22

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

, . , . , .
[2].
, , .
, 1 ,
100 0,5 . .
0,25 , 0,2-0,315 . . 2.
2
Table 2

1
2
3
4
5
6
7
8
/
Cd
12,0
20,0
13,0
18,0
10,0
52,0
14,0
14,0
0,02
Pb
0,37
0,06
0,01
0,05
0,03
0,075
0,09
0,07
0,05
Ni
0,91
0,61
0,45
0,40
0,18
0,18
0,38
0,15
0,01
Cr
8,00
3,53
2,11
2,71
2,5
1,31
1,44
1,72
1,25
Cu
1,14
0,87
0,14
0,13
0,01
0,03
0,01
0,02
0,01
Zn
5,61
13,7
8,06
7,94
1,94
0,61
4,53
2,08
0,03
Fe
0,30
2,5
0,30
0,54
0,15
0,25
0,12
0,19
52,0
. 1 ; 2 + 3 % ; 3 + FeCl3 + K4[Fe(CN)6] ; 4 + 3 % + FeCl3 + K4[Fe(CN)6]; 5 ; 6 + 3 %
(); 7 + 3 % (); 8 (0,05-0,2 ) +
FeCl3 + K4[Fe(CN)6]

,
,
.
( ) (0,05-0,2 ), FeCl3 + K4[Fe(CN)6]. ,
, , .
, , , . + FeCl3 + K4[Fe(CN)6] + 3 % + FeCl3 + K4[Fe(CN)6], . (0,05-0,2 ), FeCl3 + K4[Fe(CN)6], ,
3 % ().
:
1)
;
23

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

2) , ,
,
, : ,
, .

1. .. :
[] / .. , .. . .: , 2005.
204 .
2. ..
[]: . / .. , .. , .. . , , 2006. 97 .
: , ,
, e-mail: lbyankina@mail.ru;
, , , e-mail: natalyvaskov@yandex.ru;
, , e-mail: khalch@mail.ru.

24

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

594
..
,
690087, . , . , 52
CORBICULA JAPONICA
Corbicula japonica.
. ,
. , . .
: , , , , .

G.G. Kalinina
SPERMATOGENESIS OF CORBICULA JAPONICA
Gonad morphology of male Corbicula Japonica was investigated. There were selected five stages
of gonad maturation. Stages of active gametogenesis, prespawning and spawning of males are partly
synchronized and pass in a very short time. When spawning begins in July gametogenesis doesnt stop
and spermiogenesis occurs till the mid-August. Dynamic of gonads cell composition and ultrastructure of sperms were also investigated.
Key words: gametogenesis, spermiogenesis, gonad, ultrastructure, sperms.

. - .
. ; ,
.
. 1995-1996 .
1,0-1,5 . 0,5
. 3
[2].
.
710, , .
, I II, ,
. -4, -2 .
2%- 0,5
, 2,9%- NaCl, 1%- OsO
. JEM-100 B [2].
[3].
,
.
, , .
25

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

, .
,
, . . ,
, , ,
.
. ,
.
.

, ().
. .
.
, . (. 1, , ).
. (. 1, ).
. . . , (. 1, ).
.
, . I . . .
(. 1, ).
, , . . .
; .
, , .
(. 2).

,
, .
[1, 5].
, , , [1].
.
26

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. 1.

(): ;
;
;
;

Fig.1. Testis Corbicula Japan
in different seasons (stages):
a poslenerestovaya;
the beginning of
gametogenesis; active
gametogenesis;
perednerestovaya;
spawning

. 2.
:
1 ;
2 ;
3
.
,
(. .)
Fig. 2. Morphometric characteristics
of the testes Corbicula Japan for a year:
1 the average area of follicles;
2 the average area of the growth zone;
3 the average area of the formation
zone. Along the horizontal axis
months, along the vertical axis
ploschadt

27

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

8 1,5 . 0,7 ,
. , ,
. ,
, . . 60 (. 3).

,
.

Maturity stage of testes Corbicula Japan for a year

- -
;

;
,
;

- ;
,
,




, ;
,

- ,

;

;

;

-
,
; - ;

28

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. 3.
: ;
; ;
;
; ;

Fig. 3. Scheme sperm Corbicula Japanese:
akrosomny bubble; distal centriole;
flagellum; mitochondria;
postakrosomny material;
proximal centriole; sattelity

1. ..
[] / .. , .. // . 1985. . 1,5, 5. . 783-740.
2. .. /. .. . .: ,
1969. 420 .
3. .. / .. . .: - , 1970. 367 .
4. .. [] / .. , .. . : , 2002. 145 .
5. Franzen A. Ultrastructural studies of spermatozoa in three Bivalvia species with on
evolution of elongated nucleus of primitive spermatozoa // Gamete Res. 1983. Vol. 7.
P. 199-214.
: , , .

29

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

639.3/.6
.. 1, .. 1,2, .. 2
1
,
690087, . , . , 52
2
. .. ,
690041, . , . , 17


( , )

.
2010 . .
Foraminifera, Harpacticoida, Nematoda, Ostracoda; Polychaeta, Bivalvia.
: , , , .

O.V. Shatkovskaya, S.I. Maslennikov, L.S. Belogurova

COMMUNITY OF MEIOBENTHOS IN THE ZONE OF SUSPENDED SCALLOP
PLANTATION, BAY OF SEVERNAYA, BAY OF SLAVYANSKII
(PETER THE GREAT BAY, SEA OF JAPAN)
We studied the community of meiobenthos of soft soils in the area overhead plantation in the bay
scallop in North Slavic Bay. Research was conducted in July 2010. Meiofauna was represented by 6
groups of animals. Evmeyofauna include Foraminifera, Harpacticoida, Nematoda, Ostracoda;
pseudo-meyofauna represented immature Polychaeta, Bivalvia juveniles.
Key words: meiobenthos, suspended mollusk plantations, yesso scallop.

, [1, 2].
. . .

.
,
2010 . , 10 (. 1). .

20 2, 5 .
. 9
. [3]. 50 .
.
30

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. 10 2. .

. 1. - : 1-3 -

Fig. 1. A schematic map of the study area: 1-3 - the sampling stations in the area
suspended facilities of mariculture

6 . : Foraminifera, Harpacticoida, Nematoda, Ostracoda; Polychaeta, Bivalvia (, . 2, 3).
.
(./ 10 2)

Density of meiobenthos (ind / 10 cm2) area of yesso scallop suspended facilities in bay
of Severnaya

Nematoda
Foraminifera
Harpacticoida
Ostracoda
Polychaeta
Bivalvia

1
4,2
-

2
314,7
5,8
18,6
3,9
-

31

3
138,1
18,3
0,8
7,2

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

Foraminifera
2%
Harpacticoida
5%

Polychaeta
1%

Polychaeta
0%

Bivalvia
0%
Ostracoda
0%

Nematoda
92%

Foraminifera
11%

Bivalvia
4%

Ostracoda
1%

Harpacticoida
0%

Nematoda
84%

.2.
( 2)
Fig. 2. Composition of meiobenthos plantations
(station 2)

. 3.
( 3)
Fig. 3. Composition of meiobenthosat
(station 3)

1, - ,
Nematoda (. ). ( 2) Nematoda, Harpacticoida (. . 2, ). 3 - Nematoda Foraminifera (. . 3).
, ,
. , 84 100 % (. . 2, 3). Nematoda .
314,7 ./10 2.
, . 2 11 %
(. . 2, 3). ( 3) 18,3 ./10 2.
18,6 ./10 2.
5 % (. . 2, 3).
(0,8 ./10 2)
1 % (. . 2, 3). 3 (. . 1).
1 4 % . .
3, ( 2).
. 4.
.
1
4,2 ./10 2. . 343 ./10 2. ,
, .
.
32

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. 4.
.
Fig. 4. The total density of meiobenthos in area suspended consruction in the yesso scallop
in Severnaya bay Slavic Bay

, .
. .
. (343 ./10 2) , (4,2 ./10 2)
.

1. ..
- (. , ) [] /
.. , .. // : . ., 70- .. . : , 2008. . 705-708.
2. .. ( , ) [] / .. , .. // . 20. : , 1987. 49 .
3. .. [] / .. // . . 1971. . 7, 2. . 132-136.
: , , - , e-mail:
shatkovskaya@inbox.ru;
, , , ;
, , .

33

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

581:582.252
.. 1,2, .. 1,2, .. 1
1
,
690087, . , . , 52
2
. .. ,
690041, . , . , 17

( ) 2008, 2009 .
.
2008, 2009 . 6
: Pseudo-nitzschia pungens P. delicatissima, Dinophysis acuminata, D. acuta, D. fortii Prorocentrum minimum.
Pseudo-nitzschia 2009 .,
108,5103 ./. D. acuminata,
(200500 ./) 10 : 2008 . 2,5103 ./, 2009 . 5103 ./. P. minimum 2008 . 36103 ./ 2009 . 5103 ./.
: , , ,
.

O.G. Shevchenko, S.I. Maslennikov, T.V. Blozhko

MONITORING OF POTENTIALLY TOXIC PHYTOPLANKTON SPECIES
IN SEVERNAYA BIGHT (SLAVYANSKII BAY) IN 2008, 2009
The study of phytoplankton in the area of mollusk farm of FESTFU in Severnaya Bight (Slavyanskii Bay) was investigated from May to November 2008, 2009. Six species of microalgae known to be
toxic were found: diatoms Pseudo-nitzschia pungens and P. delicatissima, dinoflagellates Dinophysis
acuminata, D. acuta, D. fortii and Prorocentrum minimum. The massive development species of the
genus Pseudo-nitzschia (108,5103 cells/L) was observed in September 2009. The greatest density of
D. acuminata was recorded in June 2009 (5103 cells/L) it is exceeded safety level concentration of
potentially toxic Dinophysis cells for mollusk farm in some foreign countries. The maximum density of
P. minimum (36103 cells/L) was observed in July 2008.
Key words: phytoplankton, potentially toxic phytoplankton species, aquaculture, Severnaya
Bight.

. -
. ., [1]. .
. , . , ., ,
[2-4].
, , . ,
.
. 2004 . - . 34

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

[5-7]. , (. ), 30 , , [8]. , .
.
[9-11]. : - , , , , , -
[10, 12].


2008-2009 .

, , .,
.. (. 1).

. 1. - : 1-3
Fig. 1. The map of study area: 1-3 sampling stations

35

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

1-4 - 2008-2009 . . , .
,
0,07 . , 20 %
[13].

Olympus BX41 (, ). [14].
[15] , : ()
< 3104 ./; () 3104 3106 ./; -
() > 3106 ./.

6 : Pseudo-nitzschia pungens P. delicatissima, Dinophysis acuminata, D. acuta, D. fortii Prorocentrum minimum.
Pseudo-nitzschia , . [16]. P. pungens P. delicatissima .
Pseudonitzschia 16-24 . 2008 .
P. pungens 223 ./. 2009 . P. pungens 250 35,4103 ./.
, 15 % (. 2). P. delicatissima 100-75,3103 ./.
2009 . 17,8 ( 3) (. 3).
P. delicatissima 51 %
. Pseudo-nitzschia. , P. pungens 2008, 2009 ., P. delicatissima 2009 .

Pseudo-nitzschia 500103 ./,
[12]. 2009 . Pseudo-nitzschia 108,5103 ./.
Dinophysis 1524 . Dinophysis (DSP).
, DSP . ,
Dinophysis Prorocentrum [11, 17, 18].
36

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. 2. P. pungens 1-3 2009 .

Fig. 2. The seasonal dynamics of P. pungens density on 1-3 stations in Severnaya Bight in 2009
80000
70000

, /

60000
50000
40000
30000
20000
10000
0
3

24

28

. 3. P. delicatissima 1-3
2009 .
Fig. 3. The seasonal dynamics of P. delicatissima density on 1-3 stations in study area in 2009

D. acuminata
, 90 5103 ./ (. 4, 5). 2008 2009
. D. acuminata (t = 16-21 )
( 3). 5103 ./ 2009 .,
21 % . D.
fortii (t = 15 ) 21-180 ./. D. cuta
2008 . 57 ./.

Dinophysis. , D. acuminata D. fortii . D. cuta 2008 .
37

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

Dinophysis sp. 200-500 ./ [12].

D. acuminata, (200-500 ./)
10 : 2008 . 2,5103 ./, 2009 . 5103 ./.

. 4. D. acuminata 1-3 2008 .

Fig. 4. The seasonal dynamics of D. acuminata density on 1-3 stations in Severnaya Bight in 2008

. 5. D. acuminata 1-3
2009 .
Fig. 5. The seasonal dynamics of D. acuminata density on 1-3 stations in study area in 2009

Prorentrum minimum
, . 70 36103 ./.
P. minimum ( 3) :
36103 ./ 2008 . (. 6), 5103
./ 2009 . (. 7). P. minimum
. . , [19]. P. minimum
, [20].
38

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. 6. P. minimum 1-3 , 2008 .

Fig. 6. The seasonal dynamics of P. minimum density on 1-3 stations in Severnaya Bight in 2008

. 7. P. minimum 1-3 , 2009 .

Fig. 7. The seasonal dynamics of P. minimum density on 1-3 stations in Severnaya Bight in 2008

- 52 0,4106 ./ (. 8, 9), .
, . , , - [21].
. . (. ) [8, 22]. - .
, ,
.
, - 2008, 2009 .
.
39

 . 24
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. 8. 1-3
- 2008 .
Fig. 8. The dynamics of phytoplankton density in study area on 1-3 stations from May
to November 2008

. 9. 1-3
- 2009 .
Fig. 9. The dynamics of phytoplankton density in study area on 1-3 stations from May
to November 2009

6 : P. pungens, P. delicatissima, D. acuminata, D. acuta, D. fortii P. minimum. Pseudo-nitzschia . Pseudo-nitzschia
, 108,5103 ./ Pseudo-nitzschia 500103 ./,
. Dinophysis . D. acuminata 10 ( 200-500 ./),
40

.
______________________________________________________________________________________________________________________________________________________________________________________________________________________________
______________________________________________________________________________________________________________________________________________________________________________________________________________________________

. 2008 . D. acuminata 2,5103 ./, 2009 . 5103 ./. P. minimum

. 70 36103 ./,
. -


.

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Tkalin A.V., Belan T.A., Shapovalov E.N. Mar. Pollut. Bull., 1993. Vol. 26, 8.
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( ) [] / .. , .. , .. //
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3. .. -
[] / .. , .. // . . 1998. . 24, 4. . 205-211.
4. .. , Oxyrrhis marina Dujardin, 1841 [] / .. ,
.. , .. // . . 2004. . 30, 1. . 68-71.
5. .. ( )
20 [] /
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. . 2002. . 28, 2. . 107-112.
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Q. Copenhagen: Fish Inspection Serv. 1989. 61 p.
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[] / J. World Aquacul. Soc. 1990. Vol. 21. . 65-104.
11. Taylor J.R. Taxonomy of harmful dinoflagellates [] / Taylor J.R., Fukuyo Y.,
Larsen J. Manual on harmful marine microalgae. Paris: IOC of UNESCO, 1995.
. 283-317.
12. Andersen P. Design and implementation of some harmful algal monitoring systems
[] / IOC Techn. Ser. UNESCO. 1996. 44. . 1-102.
13. ..
[] / .. // . . 1984. 1. . 13-23.
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14. .. Dinphyta [] /
.. , .. // . . 1994. . 79, 6. . 117-121.
15. Yamada M. A list phytoplankton as eutrophic level indicator [] / Yamada M.,
Tsuruta A., Yoshida Y. Bull. Jap. Soc. Sci. Fish. 1980. Vol. 46, 12. . 1435-1438.
16. .. Pseudo-nitzschia (Bacillariophyta) [] / .. , .. ,
.. // . . 2001. . 27, 6. . 416-420.
17. Lee J.S. Determination of diarrhetic shelfish toxins in various dinoflagellate species
[] / Lee J.S., Igarashi T., Fraga S., Dahl E., Hovgaard P., Yasumoto T. J. Appl. Phycol. 1989. 1. . 147-152.
18. Marcaillou-Le Baut, C. Dinophysis acuminata distribution and specific toxin content
in relation to mussel contamination [] / Marcaillou-Le Baut C., Gentien P., Lunven M.
et al. Harmful Algae Blooms. 2000. IOC UNESCO. . 356-359.
19. .. Prorentrum
minimum [] / .. // . .
1994. . 20, 6. . 419-425.
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toxins in relation to the mussel fisheries 19911994 [] / Emsholm H., Andersen P.,
Hald B. Harmful and toxic algal blooms // IOC of UNESCO. 1996. . 15-18.
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6. . 403-406.
22. ..
[] / .., .. // . .
2005. . 31, 1. . 11-16.
: , , e-mail: 713553@mail.ru;
, , e-mail:
aqua@imb.dvo.ru;
, ; e-mail: tanay.beauty@ mail.ru.

42