Lymphatic &

Respiratory
System
Group D
Alex ~ Carlos ~ Dominique ~ Eren ~ Harris ~
Hector ~ Jessica ~ Marlon ~ Melanie
A lymph node is stationed along
the lymphatic system, filtering
out antigens and preparing
lymphocytes in return. Lymph
enters from afferent ducts to
subcapsular sinus into the node
proper, through the follicles of
the outer cortex, through the
inner cortex, into the medulla,
and out the hilus and efferent
vessel. Lymphocytes are added
while the lymph flows through.

4x 3x4 3-channel brightfield
image montage. R: 9 ms.
G: 16 ms. B: 12 ms.
Lymph node
Harris
Outer
Cortex
Subcapsular
Sinus
Inner
Cortex
Lymphatic follicle
Medulla
Germinal center
Capsule
Afferent
duct
Efferent
duct
left, lymph node medulla, 10x.

bottom, detail showing the reticular
tissue stained blue.
Lymph node medulla Harris
Medullary
cord
Reticular
tissue
Lymphocytes

SPLEEN
Function: Giant lymph node for blood, not for lymph.

Filters blood
Reservoir for blood
Site of immune responses to bloodborne antigens
Macrphage breakdown of hemoglobin of worn-out erythrocytes
Hemopoietic organ in fetal life that ceases after birth

Anatomy:

Red pulp- dense network of reticular fibers
Venous sinuses
Splenic cord

White pulp- Consists mainly of lymphatic tissue
Lymphatic nodule
Germinal center
Central Artery

Largest lymphoid organ
Not essential for life

SPLEEN
A B
C
D
Leica
A, BF 10x, Exp. 18.2ms, Gain. 1.0x
Sat. 1.05 Gamma 0.73
B. BF, 10x, Exp. 22.1ms, Gain 1.0x,
Sat. 1.05, Gamma 0.73
C. BF, 40x, Exp. 188.7ms Gain 1.0x
Sat. 1.15, Gamma 0.73
D. UV channel 40x, Exp. 1.0s, Gain 1.0x
Sat. 1.15, Gamma 0.73
Lymphatic nodule

Central Artery
Spleneic cord (Billroth)
Venous sinuses
Thymus
The thymus is a primary lymphoid
organ found within the superior
mediatinum, behind the upper part
of the sternum. This organ is
active in children, but at the start
of puberty, until old age, it starts to
atrophy, producing fewer T-cells. It
also produces thymic hormones
that support the growth and
differentiation of T-cell
progenitors.This is an endocrine
gland because it produces a
hormone, thymosin that promotes
the maturation of T lymphocytes.

Leica DM500, Mag:10x
BF:1.59ms, G:1.0, S:1.00, Ga:1.28
R:2.65.6ms, G:1.0, S:1.00, Ga:1.11
G:2.0s, G:1.0, S:1.00, Ga:1.48
Overlay
Lymphatic Vessel Carlos
Lymphatic vessels are
found in close association
to the cardiovascular
system. They convey fluids
from the tissues to the
bloodstream.

The red V in the vessel is
a valve
Lymphatic Vessel
Cy3 2.5x
Cy3 10x
DIC 10x
B
Palatine Tonsil
Blue Fl. 100x Oil RGB Overlay 100x OIl
Green Fl. 100x Oil Red Fl.
100x Oil
Brightfield 100x Oil
Functions:
- Located on each side of the oral
pharynx in the back of the throat
- Part of the immune system
- Assists in defending the body from
respiratory infections
Epithelial Nuclei
Crypt
Lumen
Lymphocyte Nuclei
Dominique
Trachea
The trachea is the windpipe. Hyaline cartilage
rings protect it from collapsing.
20x 3-channel brightfield overlay, 3x2
montage. R: 32 ms. G: 60 ms. B: 46 ms.
pseudostratified
epithelium

hyaline
cartilage
lamina propria

submucosa
Trachea
20x 2-channel fluorescent overlay, 3x2
montage. R: 165 ms. G: 340 ms.
Alveoli
Alveoli are the tiny sacs where the gas exchage
happens in the lungs. They are lined with simple
squamous epithelium. Alveoli are clustered into
alveolar sacs, which in turn are connected to each
other via alveolar ducts. The ducts are connected
to respiratory bronchioles.
20x 3-channel brightfield 2x2 montage. R: 16 ms.
G: 33 ms. B: 24 ms. Contrast-stretched.


alveolus

alveolar sac
alveolar duct

terminal bronchiole
respiratory bronchiole
Alveoli
20x 2-channel fluorescent overlay, 2x2
montage. R: 1.14 s. G: 2.7 s.
Bronchus
Bronchi are the last part of the conducting zone,
bringing air into the lungs. They are smooth
muscle with hyaline cartilage rings. The smooth
muscle is lined with pseudostratified epithelium.

20x 3-channel brightfield 2x2 montage. R: 16 ms.
G: 33 ms. B: 24 ms. Contrast-stretched.

smooth muscle
epithelium
Bronchus
20x 2-channel fluorescent overlay, 2x2
montage. R: 770 ms. G: 1.15 s.