Q1.

Objective Type Questions

10X0.5=5

1. Which of the following is not comes under organ -----------a) ovary culture

b) Nucellus culture

c) Anther culture

d) None

2. Ovary culture has been used successfully to develop ----------a) Polyembryony

b) Monoembryony

c) haploids

d) All

3. In most of the cases endosperms are produced by ----------a) Fission of one polar nuclei and one male gamete
b) Fission of two polar nuclei and one male gamete
c) Fission of one polar nuclei and two male gamete
d) Both a & b
4. Outermost layer of plant cell wall is ------------------5. Fusion of Two protoplast is called -----------------------a) Fertilization b) Somatic hybridization

c) Double fertilization

d) Triple fusion

6. Primary cell culture means -------------a) Culture of cells

b) Culture of tissues

c) both

d) None

7. After isolating the tissue cells are disaggregated in ---------- manner

a) Mechanical disaggregation

b) Enzymatic disaggregation

c) both

d) None

8. Steps to establish primary culture is -------a) Isolation of tissues

b) Dissection/ disaggregation

c) Culture following seeding into culture vessel

d) All

9. The filter used in laminar air flow is ----------------------10. In animal cell culture the incubator used are of --------- type
a) BOD incubator

b) Shaker Incubator

c) CO2 Incubator

d) Platform shaker

Q 2. Short Answer Type Questions (attempt any four)

4X2.5=10

1. Write short notes on Synchronization of suspension culture cells

2. Write short notes on Bergmann cell plating techniques
3. Define primary cell culture
4. Write short notes on continuous cell culture
5. Describe in brief the growth kinetics of cell line
6. Describe the different media and supplements in cell culture
Q 3. Long Answer Type Questions (attempt any three)
1. What is somatic hybridization? Explain different method of

5X3=15
somatic hybridization and

discuss its significance

2. What is suspension culture? Explain the different types and discuss the significance of
suspension culture
3. Discuss organ culture in details
4. Explain the different components of laboratory organization
5. Describe trypsinization in details