MULTIPLE EXTRACTION OF CAFFEINE FROM DRIED TEA LEAVES USING

DICHLOROMETHANE

AUTHORS: Dizon, S., Donato, A.K., Dy, A., Enriquez, K.B., Fauni, M.B.

ABSTRACT:

Caffeine was extracted from dried tea leaves by multiple extraction technique. 10
g. of tea leaves was boiled in a solution of 4.4 g. anhydrous sodium carbonate and 100
ml distilled water and was extracted three times using 20 ml of dichloromethane. The
residue was collected by decanting the mixture of organic extract and anhydrous sodium
sulfate and evaporating it to dryness. The percentage yield was computed by getting the
ratio of the weight of the crude caffeine (residue) and weight of the tea leaves used. The
percentage yield was 5.33%.

I.)INTRODUCTION II.) EXPERIMENTAL

Extraction is the process of A.) Weight of specimen (before

obtaining something from a mixture or extraction)
compound using chemical, physical, or
mechanical means. The theory of The weight of tea leaves used and
extraction lies in the concept of empty evaporating dish was obtained using an
immiscibility between two phases to analytical balance.
separate a solute from the other phase.
There are two main types of extraction B.) Extraction
in chemistry namely: Liquid-liquid
A solution of 4.4 g. anhydrous sodium
extraction (also known as Solvent
carbonate and 100 ml distilled water in an
extraction or Partitioning), which is
Erlenmeyer flask was heated in a water bath
further divided into two types (simple
until the anhydrous sodium carbonate
and multiple), and Solid-liquid
dissolved. 10 g. of tea leaves contained in a
extraction. The type of extraction used
tea bag was added to the mixture. The tea
in this experiment was Multiple Liquid-
mixture was left to boil and upon the first sign
liquid extraction. In this experiment, the
of bubbles, the tea mixture was timed to boil
group should be able to attain the
for 10 minutes. The tea bag was removed
following objectives: (1) extract caffeine
from the Erlenmeyer flask and its excess liquid
from dried tea leaves (2) know the
was collected by pressing it against the side of
amount of caffeine present in 10.3095
the flask using a glass rod.
g. of tea leaves, and (3) calculate the
percentage yield of caffeine in 10.3095 20 ml dichloromethane was added to
g. of tea leaves. the aqueous solution in a separatory

funnel. It was left to stand for 2

minutes (or more) until the separation
between two layers was clearly visible.
The organic layer was drained into a
clean Erlenmeyer flask and the aqueous
layer was discarded. This procedure was
repeated for three times and all the
organic layers obtained were combined.

Half a spatula of anhydrous sodium

sulfate was added to the extract in an
Erlenmeyer flask. The solution was
decanted onto a tared evaporating dish
and was evaporated to dryness.

Fig. 1 (Extraction Set-up)

C.) Weight of specimen (after
extraction)

The weight of the evaporating

dish containing the crude caffeine
(residue) was obtained using an
analytical balance. The weight of the
crude caffeine was obtained by
subtracting the weight of the empty
evaporating dish to the weight of the
evaporating dish containing the crude
caffeine (residue).

D.) Percentage yield

Fig. 2 (Schematic diagram)

The percentage yield was obtained

by getting the ratio of the weight of the
crude caffeine (residue) and the weight
of the tea leaves used, then multiplying
it by 100%.

III.) RESULTS AND After subjecting the tea mixture to the

DISCUSSION entire process of extraction, decantation, and
evaporation, crude caffeine was successfully
collected. 0.0550 g. of crude caffeine was
collected from 10.3095 g. of tea leaves. A
WEIGHT OF TEA 10. 3095 g. percentage yield of 5.33% was computed after
LEAVES USED getting the ratio of the weight of caffeine
(residue) and weight of tea leaves used then
WEIGHT OF 130.7495 g.
multiplying it by 100%. This means that for every
EVAPORATING
10.3095 g. of tea leaves, an amount of 0.00550
DISH +
g. of caffeine can be collected using the technique
CAFFEINE
of multiple liquid-liquid extraction and this
(RESIDUE)
0.00550 g. of caffeine constitutes 5.33% of the
tea leaves.
WEIGHT OF 130.6945 g.
EMPTY
EVAPORATING
DISH IV.) REFERENCES

WEIGHT OF 0.0550 g. http://www.chemicool.com/definition/extraction.h

CAFFEINE tml
(RESIDUE)
Date accessed: 07/ 27/ 2009 8:58 P.M.
PERCENTAGE 5.33%
YIELD http://orgchem.colorado.edu/hndbksupport/ext/e
xt.html
Fig. 3 (Data obtained)
Date accessed: 07/ 27/ 2009 9:01 P.M.

http://www.thefreedictionary.com/extraction

Date accessed: 07/ 27/ 2009 8:56 P.M.

http://www.tutorbene.com/cms_images/LIQUID-
LIQUID%20EXTRACTION.bmp

Date accessed: 07/ 27/ 2009 9:19 P.M.

Fig. 4 (Claculations)