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PROJECT OVERVIEW

Name of Project:

Duration: 4 weeks

Genetic Engineering

Subject/Course: Biotechnology

Teacher(s): M. Penarroyo

Grade Level: 12

Other subject areas to


be included, if any:

Project Idea
Summary of the issue,
challenge, investigation,
scenario, or problem:

Students will perform several lab procedure used in genetic engineering process. Students will use the AMGEN
protocol.

Driving Question

Is genetic engineering a cure or a treatment?

Content and Skills


Standards to be
addressed:

HS-LS1-1. , HS-LS1-6. , HS-LS3-1. , HS-LS3-2. , RST.11-12.9, WHST.9-12.1, WHST.9-12.9

T+A
21st Century Skills
to be explicitly taught and
assessed (T+A) or that
will be encouraged (E) by
project work, but not
taught or assessed:

T+A

Collaboration

Problem Solving

Presentation

Analysis

Critical Thinking:

Presentation Audience:

Group:
Culminating
Products and
Performances

Lab results plate colony of bacteria, gel electrophoresis

Class:
School:
Community:
Experts:

Individual:

Web:
2008 Buck Institute for Education

Other:

PROJECT OVERVIEW
Entry event to
launch inquiry,
engage students:

https://www.youtube.com/watch?v=FBcyUKz_8cA

Assessments

Quizzes/Tests
Formative
Assessments
(During
Project)

Practice Presentations

Journal/Learning Log

Notes

Preliminary Plans/Outlines/Prototypes

Checklists

Rough Drafts

Concept Maps

Online Tests/Exams

Other:

Written Product(s), with rubric:


Summative
Assessments
(End of Project)

Other Product(s) or Performance(s), with rubric:

_Lab report

__________________________________________________

Oral Presentation, with rubric

Peer Evaluation

Multiple Choice/Short Answer Test

Self-Evaluation

Essay Test

Other:

Resources
Needed

Reflection
Methods

On-site people, facilities:

Equipped laboratory

Equipment:

Different lab equipment supplied by AMGEN & those present in the lab

Materials:

Misc. lab supplies, reagents, plastic ware, glassware etc

Community resources:

AMGEN

(Individual,
Group, and/or
Whole Class)

Journal/Learning Log

Focus Group

Whole-Class Discussion

Fishbowl Discussion

Survey

Other:
2008 Buck Institute for Education

PROJECT TEACHING AND LEARNING GUIDE


Project:

Course/Semester:
Knowledge and Skills Needed by Students
to successfully complete culminating products and
performances, and do well on summative assessments

Students learn to use two basic biotechnology lab tools the micropipette
and gel electrophoresis)

students make the recombinant plasmid of interest from two starting


plasmids and use it to create a genetically modified organism)

students prepare an overnight culture of E. coli and purify red fluorescent


protein from lysed cells)

Scaffolding / Materials / Lessons to be Provided


by the project teacher, other teachers, experts,
mentors, community members

Laboratories 1.1 and 1.2

Laboratories 25

Protein purification labs

2008 Buck Institute for Education

P R O J E C T
Project: Genetic Engineering
M O N D A Y

C A L E N D A R
Start Date: September 2014

T U E S D A Y

W E D N E S D A Y

T H U R S D A Y

F R I D A Y

PROJECT WEEK ONE


Introduction
Project Overview

Pre-lab for Lab 1.1 &


1.2

PROJECT
Lab 1.2

Lab 1.1.

WEEK

Post lab 1.2


Prelab 2

Prelab 3

TWO
Lab 2

PROJECT

WEEK

Post lab 1.1

Lab 2

THREE
Lab 3

Lab 3

Post lab 2

2008 Buck Institute for Education

P R O J E C T
Project: Genetic Engineering
M O N D A Y

C A L E N D A R
Start Date: September 2014

T U E S D A Y

W E D N E S D A Y

T H U R S D A Y

F R I D A Y

PROJECT WEEK FOUR


Pre lab 4

Pre-lab for Lab 4

Lab 4

PROJECT
Prelab 5

Prelab 5

Prelab 6

FIVE
Lab 5

PROJECT
Overnight culture of E.coli

WEEK

WEEK

Post lab 4

Post Lab 5

SIX
Lab 6

Postlab 6

2008 Buck Institute for Education

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