Preparation of FeEDTA Solution

To prepare FeEDTA solution, sodium salt of EDTA and ferrous sulfate along with NaOH
solution is used. To prepare 1 L of FeEDTA solution, the following composition and procedure
should be adopted:
1. Dissolve 33.2 g NaEDTA salt (C10H14N2Na2O8 2H2O FW 372.24 g mol1) in about 200
mL of deionized water.
2. Make an 89.2 mL solution of 1 N NaOH.
3. Dissolve 29.4 g of FeSO4 7H2O in about 100 mL of deionized water.
4. Mix NaEDTA and NaOH solutions slowly and stir. Then, mix this solution with FeSO 4
7H2O solution. Leave this solution overnight in a dark ambience. Next day, complete the
volume to 1 L. This solution will contain 106 mM or 5936 ppm Fe. According to Novais
et al. (1991), FeEDTA solution can also be prepared by mixing 14.1 g of Na 2 EDTA and
10.3 g of FeCl3 6H2O, diluted separately in about 300400 mL of deionized water and
then mixed together to make a volume of 1 L. This solution contains 38 mM Fe. Care
should be taken while chelating iron used in nutrient solution.