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Activity No. 09
ACETONE
I. Objectives:
1. To detect the presence of acetone in a sample by performing Lieben’s
Iodoform test, Legal’s test, and Reynold’s test;
2. To understand the principles behind each test, and
3. Interpret the results after performing each method of detection.
I. Data:
Method of
Reagents Observations and Results
Detection
Lieben’s Iodoform Lugol’s solution Actual: Acetone + 1 ml Lugol’s sol’n
Test (Aqueous iodo- Dark brown; Acetone + 1 mL of
potassium lugol’s sol’n + KOH Colorless; The
iodide solution), solution turned yellow after heating.
potassium Ideal (+): Iodoform immediately
hydroxide separates, even in the cold, as a
yellowish white precipitate which is
usually amorphous (Autenrieth,
1915).
Legal’s Test 0.5% sodium Actual: 2 mL acetone + 2 gtt 0.5%
nitroprusside sodium nitroprusside sol’n rusty
solution, red at the bottom.; 2 mL acetone +
potassium 2 gttd o.5% sodium nitroprusside +
hydroxide conc. NaOH sol’n violet sol’n. The
solution turned light blue green after
it was acidified with acetic acid.
Ideal (+): A reddish yellow color
appears after the addition of 0.5%
sodium nitroprusside solution to the
liquid containing acetone and
potassium hydroxide. The solution
changes its color to carmine to
purplish red color upon the addition
of acetic acid. Heating the solution
changes its color to violet
(Autenrieth, 1915).
Reynold’s Test Mercuric Actual: Acetone + 2 gtts mercuric
chloride, chloride + alcoholic KOH clear
alcoholic KOH solution. The solution formed a layer
solution, at the top of he clear solution.
ammonium Ideal (+): If acetone is present, there
sulfide will be a black zone (HgS) where two
solutions meet (Autenrieth, 1915).
Data Analysis
Lieben’s Iodoform Test
The red color caused by aldehyde fades upon addition of acetic acid,
and changes to green with heat. Le Nobel states that ammonium hydroxide,
or ammonium carbonate solution, may be substituted for potassium
hydroxide solution in Legal’s test, but under these conditions the red color is
very slow to appear.
Answers to Questions:
1. What are the long term health effects of exposure to acetone?
2. Will acetone act in a synergistic manner with other materials (will its effects
be more than the sum of the effects from the exposure to each chemical
alone?
I. Conclusion/s:
II. Bibliography:
Autenrieth, W. (1915). Laboratory Manual for The Detection of Poisons and Powerful
Drugs. (W. H. Warren, Trans.) Philadelphia: P. Blakiston's Son & Co.
Canadian Centre for Occupational Health and Safety. (1997, December 18). 2-
Health Effects of Acetone. Retrieved January 04, 2010, from ccohs.ca:
http://www.ccohs.ca/oshanswers/chemicals/chem_profiles/acetone/health_ace.html
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