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Objectives
To measure gene expression of the Ced-3
before and after treatments with glyphosate.
We will use this data to determine if the gene
is being overexpressed, under expressed, or
not changing. The Ced-3 gene is responsible
for programmed cell death (apoptosis) in C.
elegans. .
Methods
Results
Discussion
References
Analysis
To quantify data a technique called real time
PCR was used. This techniques allows one to
replicate specific DNA sequences. With every
cycle of the machine our specific sequence
was replicated, and tagged with a fluorescence
(SYBR green). The real time PCR machine is
able to measure the abundance of this
fluorescence after each cycle, and give real
time feed back. That is how we were able to
see if our gene was up regulated in the worms
treated with glyphosate vs. the control (treated
with water).