REGIN-X° Red Ginger Extract , ee me 6B PT. PHYTOCHEMINDO REKSA Your Partner on feral Health, MS REGIN-X° Red Ginger Extract Ginger (Zingiber officinale) is a very popular spice widely consumed in foods and drinks since earlier times. Ginger also has a long tradition of medicinal uses. The German Commission E monograph contains reviews of herbal drugs, including ginger. In the German "Hager's Handbuck der Pharmazeuthischen Praxis", ginger is listed as being used against rheumatism, chronic inflammation of the intestines, coughs and sore throat. Red ginger (Zingiber officinale var. Rubra) is a special kind of ginger which is mostly cultivated in Indonesia, known locally as "Jahe merah". It has been proven to contain a higher concentration of the active ingredients. Besides having Gingerols and Shogaols, it is loaded with Anthocyanin and Tannin in its root bark. For the last 20 years, pharmacological studies have been performed on the contents and benefit of Gingerol, Shogaols, and Anthocyanidin. Clinical studies both in vitro and vivo have proven that these substances provide a potent anti-inflammatory agent which acts as a natural analgesic and prevents and treat © Osteoarthritis of the knee © Rheumatism/arthritis © Coughs and sorethroats Phytochemindo has developed a Red Ginger Extract with high levels of Gingerol, Shogaol and Anthocyanidin which are suitable for anti arthritic preparations. Phytochemindo's Red Ginger Extract is manufactured from select, high quality raw materials fresh from native Indonesia plants, resulting in a standardized extracts from our WHO GMP certified extraction plant. eo UU ~z 1. Acute and Chronic Inflammation The effect of Red Ginger Extract on acute inflammation was experimented on acetic acid induced abdominal inflammation model in mice. The analgesic effect of Red Ginger Extract was determined by Writhing Counts. Oral administration of Red Ginger Extract (10 - 100mg/kg) demonstrated a dose dependent pain relief effect with significant reduction in Writhing counts. Similarly, it exerted a dose-dependent anti-inflammatory effect where the amount of leaked dye, an indicator on extend of inflammation, reduced significantly at 50 and 400mg/kg. In addition, (Young H-Y et al.,) showed that intraperitoneal injection of [6]-gingerol (50mg/kg) relieved pain by 50%. As revealed in this experiment, Red Ginger Extract in low oral dose of 0.2 - 2mg/kg demonstrated effective analgesic and anti-inflammatory effects suggesting the synergisms of various physiological compounds presents in the extract. (Young H-¥, Luo ¥-L., Cheng H-¥,, Hsieh W-C, Liao J-C., Peng W-H. Analgestic and anti-inflammatory activities of (6)- gingerol. J. Ethnopharmacol. 96, 207-210 (2005))2. Anti-arthritic effect The effect of Red Ginger Extract on chronic inflammation was experimented using rat adjuvant arthritis model. This model is generally used for the diagnosis of rheumatism. Edema was induced and observed in rats treated with adjuvant (control). No significant changes observed in group treated with Red Ginger Extract 1mg/kg. However, marked anti-inflammatory effect was observed on day-13 in group treated with Red Ginger Extract 10mg/kg. Indomethacin (0.5mg/kg), a commonly prescribed NSAIDS drug was used as positive control, showing a significant anti-inflammatory effect (p<0.01) upon induction of inflammation throughout end of experiment. (Shimoda H., Shan S.J, Tanaka J, Seki A, Seo J. W., Kasajima N., Tamura S, Ke ¥., Murakami N. Anti-inflammatory properties of red ginger (Zingiber officinale var. rubra) extract and suppression of nitric oxide production by its constituents. J. Med. Food 13, 1-7 (2010)) ae mee ee 10 ene Figure 1. Figure 2. ‘The effect of Red Ginger Extract (RGE) on rat X-ray images of limbs of rat treated with adjuvant adjuvant arthritis model 3. Antioxidant The study compares the protective properties of two varieties of ginger [red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe)] on Fe2+ peroxidation in rat brain in vitro. Incubation of the brain tissue homogenate in the presence of Fe caused a significant increase in the malondialdehyde (MDA is the end-product of lipid peroxidation and the production of this aldehyde is used as a biomarker to measure the level of oxidative an organism) contents of the brain. The aqueous extract of red ginger had a significantly inhibitory effect on both Fe2+-induced lipid peroxidation in the rat brain homogenates than that of white ginger. This higher inhibitory effect of red ginger could be attributed to its significantly higher phytochemical content, Fe2+ chelating ability, OH scavenging ability and reducing power. However, part of the mechanisms ‘through which the extractable phytochemicals in ginger (red and white) induced the protect be through their antioxidant activity, Fe2+ chelating and OH scavenging ability. Therefore, oxidative stress in the brain could be potentially managed/prevented by dietary intake of ginger varieties (red ginger and white ginger rhizomes) (Ganiyu Oboh, Ayodele J. Akinyerni, Adedayo 0. Ademiluyi, Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe2+ induced lipid peroxidation in rat brain in vitro, Experimental and Toxicologic Pathology 64 (2012) 31-36)® g | omsmeren z j- ae ginger & fe | a oi a ame as Spl erat (0. 93me/mi : concentration e109 el Figure 3. Figure 4. Fe’ chelating abilty of Red Ginger and White Ginger OH radical scavenging ability of Red Ginger and White extract Ginger extract 4 Extract Specification Product Name REGIN-X" Dry Extract Paar sa ee ee eee FU cary awe Common Name i Red Ginger heal edracts accepted standardization feria co co and eartications Coen Dry Extract high concentrate | Liquid Exiract high concentrate Appearance co Dark brown Odour and Taste Spicy, Pungent Pomc Active Ingredient (Orocol Groce’ Identification fo fom ay ry Soluble in water Pe et Perea CL Heavy Metal Coe Coe) Rod Tr ar fo Br 10kg @ REGIN-X”* available grade : CU ST orca sot for Red Ginger Dry Extract 15% corr Peace corny Poca es Een fees water soluble Application of REGIN-X° © Pharmaceutical -- Red ginger oil is used in the treatment of fractures, rheumatism, arthritis, bruising, carbuncles, diarrhea, colic, and flu, sores on the skin, sore throat, catarrh, congestion, coughs, sinusitis, and fever. Red ginger is an excellent natural remedy for nausea, motion sickness, morning sickness and general stomach upset due to its carminative effect that helps break up and expel intestinal gas. © Cosmetics -- In cosmetics and essence, its can be used in a variety of ways such as room fragrances, bath oil, massage oil, perfumes, inhalations, natural preservatives and in cosmetic preparations. © Food industry -- Red ginger is used mainly as flavoring condiment and a popular spice. Red ginger is a seasoning and flavor sweets, including cakes, cookies, breads and beverages. FACTORY: Jl. Mercedez Benz No. 105 / Cicadas, Gunung Putri, Bogor - Indonesia 16964 P +6221 867 1037 - 868 62478 | F +62 21 867 1036 E, sales@phytochemindo.com www.phytochemindo.com