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sonr014 [Please select Print from the file menu to print your Abstract. Submission Number: 11691 [Title of Activity: 2015 United States & Canadian Academy of Pathology Annual Meeting [Date of Activity: March 21-27, 2015 |Contact/Presenting Author: Mostafa Belghasem M.A. linstitution: Pathology, Boston University |Address: 670 Albany street, R345 \City/State/Zip/Country: Boston, MA, 02118, United States Phone: 6174147001 Cell Phone: E-Mail: mbelgha@bu.edu Submit Info Submi: ion Category: Kidney/Renal Pathology (including Transplantation) [Presentation format: Poster Only |Authors: 17. Mostafa Belghasem M.A.', 2, Mei Cao PHD!, 3. Philip Bondzie PhD! and 4, Joel Henderson IM.D.,PhD! uthors who are Pathologists-In-Training: Institutions: Boston University, Pathology, Boston, MA, United States. [Award Information: |Award: I am NOT applying for any award Title: 18 sonr014 lPodocyte-Specific Myh9 Deletion in Mice Results in Susceptibility to Glomerular Injury in Response {to Subtotal Nephrectomy. |Submission Body: IBackground: The gene MYH9 encodes non-muscle myosin IIA, an essential podocyte contractile protein. [The increased incidence of chronic kidney disease in African Americans has been linked to genetic variation near MYH9, and rare hereditary syndromes involving mutations in MYHO often include renal linvolvement. The genetic features of these forms of MYH9-associated kidney disease suggest that altered IMYHO expression levels could play a role in disease pathogenesis. The objective of this study is to ldetermine the effect of MYH9 ablation on non-muscle myosin expression and kidney structure, and lresponse to hemodynamic stress, using podocyte-specific Myh9 knockout mice. [Design: We investigated the role of Myh9 in renal injury after subtotal nephrectomy. Podocyte-specific IMyh9 knockout mice on a CS7BL/6 background and controls were subjected to subtotal 5/6 nephrectomy for 8 weeks. During treatment, systemic blood pressure was measured by the tail cuff method, and kidney lfunction was assessed with urine and serum biomarkers. At the end of the treatment period, kidney lwas collected and kidney morphology was evaluated by light and electron microscopy. IResults: Myh9 knockout mice showed glomerular hypertrophy, podocyte foot effacement, glomerular lbasement membrane thickening and exacerbation of urinary albumin excretion, after subtotal nephrectomy |compared with control mice. In addition, the knockout mice showed persistent elevation of systolic blood pressure. |Conclusion: Podocyte-specific Myh9 ablation renders mice susceptible to glomerular injury when |subjected to 5/6 nephrectomy. This suggests that non-musele myosin ILA plays an important role in protecting the glomerulus from hemodynamic stress indeuded by subtotal nephrectomy. Nevertheless, funder normal physiological conditions podocyte-specific Myh9 ablation had no significant effect on kidney| structure and function. Disclosure Info: lis this abstract original material? Yes |Has this abstract been previously published? No lis this abstract accepted for publication or presentation at another meeting before March 27, 2015? No [Does this abstract include the use of unlabeled (or off-label) investigational products? No [Does your abstract include references to any commercial products or services? No IRB Approval: Yes I, affirm that this research study has been approved by my local Institutional Review [Board/Human Subject Research Review Committee or its designee, or that my study is exempt from such 28 sonr014 review according to the governing guidelines, [Disclosures by Author: |Author: Mostafa Belghasem [Financial Relationships(s): [Do you have any relevant financial relationships with commercial interests? |Author: Mei Cao Financial Relationships(s): !Do you have any relevant financial relationships with commercial interes INo, I do not have any relevant financial relationships with any commercial interests |Author: Philip Bondzie |Financial Relationships(s): [Do you have any relevant financial relationships with commercial interests? |Author: Joel Henderson Financial Relationships(s): [Do you have any relevant financial relationships with commercial interests? INo, I do not have any relevant financial relationships with any commercial interests INo, I do not have any relevant financial relationships with any commercial interests. INo, I do not have any relevant financial relationships with any commercial interests. a8