CLASSIFICATION OF ENZYMES

(Enzyme Comission-EC)
EC 1. OXIDOREDUCTASES
EC 1 enzyme

Type of redox
reaction

Succinatedehydrogenase
(citric acid cycle)

C=C formation

Alcoholdehydrogenase

Alcohol-aldehyde
conversion

Aldehydedehydrogenase

Aldehyde-acid
conversion

Possible reaction type

Phe hydroxylase
(monooxygenase)

EC 2. TRANSFERASES
EC 2 enzyme

Transferred group

Acyl-transferase

Acetyl, succinyl,
aminoacyl

(eg. synthesis of
triacylglycerols and
phospholipids)
Phosphotransferase

-H2PO3 (phosphoryl)

(eg. hexokinase,
glucokinase)

Amino- transferase
(eg. ASAT, ALAT)

-NH2 (amino)

Sulfo- transferase
(eg. GAG synthesis)

-SO3H (sulfuryl)

Possible reaction type

EC 3. HYDROLASES
E.C. 3 enzyme

Substrate

Esterases (eg.
Lipase,
neutral fats
phospholipids
phospholipase,
acetylcholine
acetylcholine
esterase)
PhosphodiesteNucleotides
rases (eg. cAMP
phosphodieste- (cAMP 5 AMP)
rase)

bond

Possible reaction type

ester

phosphodiester

Phosphatases
(eg. glucose-6phosphatase)

phosphatester
(glu-6-P)

phophomonoester

Glycosidases
(eg. amylase,
lipase)

polisaccharide
disaccharide

glycoside

Proteases
Peptidases
(eg. tripsin)

proteines
peptides

peptide

EC 4. LIASES
E. C. 4enzyme

Removed
group

Decarboxylase
(eg. amino acid
decarboxylase)

CO2

Aldolase
(eg. aldolase A/B)

Aldehyde

Synthase
(eg. Gly synthase)

Possible reaction type

CO2 + NH3

Dehydratase
(eg. cysthationin
synthase)

H2 O

Desaminase
(eg. Gln desaminase)

NH3

Gly

EC 5. ISOMERASES
E. C. 5
enzyme

Isomerated group

Changing
position of
the group

Glucose-6phosphateisomerase

carbonyl

C-1 C-2

Phosphoglycerate
-phosphomutase

phosphoryl

C-2 C-3

Epimerase
(eg. UDP-Glu
UDP-Gal)

hydroxyl

DL

Possible reaction type

EC 6. LIGASES
E.C. 6... enzyme

Type of bond

Example
Activation of amino acid, synthesis of
proteins

Aminoacyl-tRNS-synthetase

CO

Glutamine- synthetase

CN

Biosynthesis of glutamine

Amino acid-ligase

CN

Synthesis of proteins

Acetyl-CoA-carboxylase

CC

Fatty acid synthesis (malonylSCoA

formation)