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Trng i hc Cn Th
ABSTRACT
Three Azospirillum lipoferum strains isolated and identified by Polymerase Chain
Reaction (PCR) technique were used to test their ability of synthesis of Indole Acetic acid
(IAA) and their effects on the growth of rice root cultivated in the greenhouse.
The results showed that all of three strains R7b1, R8b2 and R29b1 could synthetized high
amout of IAA than the control in the medium without Tryptophan. Azospirillum lipoferum
strain R29b1 could synthesized the highest amount of IAA (19,9g/ml) after inoculating 4
days. These amount of IAA supported the growth of rice root length and the amount of
lateral root grown in the green house. After inoculating 28 days, the rice root length were
2.03 times compared to the control.
Keywords: Azospirillum lipoferum, Indole Acetic acid (IAA), tryptophan, rice root,
green house
Title: Effect of Indole Acetic acid (IAA) synthesized by Azospirillum on the growth of
rice root grown in the green house
TM TT
Ba dng vi khun Azospirillum lipoferum phn lp c t r la, nhn din bng k
thut PCR, c chn kho st kh nng tng hp IAA v nh hng ca chng ln s
pht trin ca r la trong iu kin nh li.
Kt qu th nghim cho thy, c ba dng R7b1 R8b2 v R29b1 u tng hp c lng
IAA nhiu hn i chng trong mi trng nui khng c Tryptophan. Dng R29b1 tng
hp c lng IAA nhiu nht 19,9g/ml vo ngy th 4 sau khi chng. Lng IAA ny
gp phn lm tng chiu di r la v tng s lng r ph trong th nghim nh li.
Chiu di r la tng 2,03 ln so vi i chng khi c chng dng R29b1 sau 28 ngy.
T kha: Azospirillum lipoferum, Indole Acetic acid (IAA), tryptophan, r la,
nh li
1 T VN
Vit Nam ang l nc ng th hai trn th gii v sn lng la go xut khu
(http://www.fita.org/countries/vietnam.html) (Truy cp vo thng 11/2009). Trong
, ng bng sng Cu Long ng vai tr rt quan trng (ng gp 90% lng
go xut khu ca Vit Nam) (http://www.tapchicongsan.org.vn/print.asp) (truy
cp ngy 11/02/2009).
Mun tng nng sut cho cy la, ngi nng dn s dng mt lng ln phn
ha hc, trong c phn m. Tuy nhin, bn cnh mt tch cc, lng phn ha
1
2
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Trng i hc Cn Th
1,5ml sinh khi vi khun ly tm 5.500 vng/pht trong 10 pht, nh nhng ht 1ml
dch trong ca mu sau khi ly tm trn vi 2ml thuc th Salkowski R2 (4,5g
Fe2Cl trong 1lt H2SO4 10,8M), mu trong ti 15 pht trc khi o OD bc
sng 530nm. Da vo ng chun c nng IAA tng dn: 0, 5, 10, 20, 30, 40
g/ml xc nh lng IAA do vi khun to ra (Eric Glickmann et al., 1995).
2.2.2 Kho st nh hng ca vi khun Azospirillum lipoferum ln chiu di r la
R29b1
R29b1
R8b2
R8b2
R7b1
R7b1
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Nng IAA
20
Ngy 1
15
Ngy 2
Ngy 4
10
Ngy 6
Ngy 8
5
0
Ab28
ab10
ab39
r7b1
r8b2
r29b1
Cc dng vi khun
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Trng i hc Cn Th
20
R29b1
10
Dng R29b1 sinh ra lng IAA cao nht l 19,9 (g/ml) vo ngy th 4 (Hnh 4)
v khc bit c ngha so vi cc dng cn li (bng 1). Trong khi , kt qu
ca Andriollo et al. (1990) cho thy IAA khong 0,5-18 (g/ml) khi b sung 50
(g/ml) Tryptophan vo mi trng nui vi khun. Cn kt qu ca Patten et al.
(2002) khi c b sung Tryptophan vi nng 500 (g/ml) vo mi trng nui vi
khun th vi khun sn sinh c lng IAA l 32,72,9 (g/ml/OD600 unit).
Khalid et al. (2001) khi phn lp mt s chng vi sinh vt t vng r ca cy la
go v la m v th kh nng tng hp IAA ca cc dng vi khun , cho thy
lng IAA tng hp c ca cc dng cao nht l 12,1 gIAA/ml. Tuy nhin,
cc dng vi khun R7b1, R8b2 v R29b1 s dng trong nghin cu ny sn sinh
lng IAA ph hp vi kt qu ca Ral Osvaldo Pedraza et al. (2004), nghin
cu cho thy cc dng Azospirillum sp. tng hp c IAA khong 16,538 g
IAA/ml.
Bng 1: Lng IAA do cc dng vi khun to ra
Dng vi khun
Ab39
Ab10
Ab28
R7b1
R8b2
R29b1
CV (%)
Trong cng mt ct nhng s c ch theo sau ging nhau th khc bit khng ngha mc 5% qua php th
Duncan.
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Nghim thc
i chng
R7b1
R8b2
R29b1
CV (%)
Chiu di r la (cm)
4,875 a
7,375 a
7,125 a
7, 625 a
29,55
Trong cng mt ct nhng s c ch theo sau ging nhau th khc bit khng ngha mc 5% qua php th
Duncan.
Chiu di R La (cm)
0
i chng
R29b1
R7b1
R8b2
n ngy th 21, nghim thc chng vi khun R29b1 cho chiu di r la cao
nht, khc bit c ngha so vi tt c cc nghim thc cn li v gp 2,03 ln so
vi i chng. Dng R7b1 v R8b2 cng gip tng chiu di r la, khc bit
mc ngha 5% so vi i chng (Hnh 7); do c ba dng vi khun ny u c kh
nng tng hp IAA v dng R29b1 to ra lng IAA nhanh hn hai dng cn li
nn gip r la di hn. Kt qu ny ph hp vi kt qu ca th nghim kho st
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Chiu di R La
lng IAA sinh ra v c Kolb v Martin (1985) chng minh bng th nghim
phun IAA c nng l 10-9g/l vo r ca cy la m kt qu l r cy di hn.
20
15
10
5
0
i chng
R29b1
R8b2
R7b1
Chiu di r la (cm)
i chng
R7b1
R8b2
R29b1
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Nghim thc
i chng
R7b1
R8b2
R29b1
CV (%)
Chiu di r la
10,25 b
18,75 a
19,75 a
20,25 a
13,39
Trong cng mt ct nhng s c ch theo sau ging nhau th khc bit khng ngha mc 5% qua php th
Duncan.
Nh vy, sau 28 ngy chng, cc dng vi khun gip tng chiu di r cy la.
c bit, dng R29b1 gip tng chiu di r la ln 2,03 ln so vi i chng. Kt
qu ny tng t nh kt qu ca Kolb v Martin (1985) khi phun dch vi khun
Azospirillum brasilense FT-326 trn r ca Beta vulgaris lm cho r mc di hn
v pht trin c cc r th cp v Amalia et al. (1988) cng chng minh
Azospirillum brasilense lm tng chiu di r Panicum miliaceum.
4 KT LUN V NGH
4.1 Kt lun
C ba dng R7b1, R8b2 v R29b1 u c kh nng tng hp IAA m khng cn
thm tryptophan vo mi trng nui. Lng IAA nhiu nht c dng R29b1
tng hp l 19,9g/ml.
Chiu di r la cc nghim thc c chng vi khun khc bit so vi i chng
mt cch c ngha. Dng R29b1 gip r la di hn 2,03 ln so vi i chng
sau 28 ngy chng.
4.2 ngh
Th nghim ngoi ng cn c tin hnh nhiu vng sinh thi khc nhau
hng n vic sn xut phn sinh hc cho cy la, xy dng nn nng nghip bn
vng, thn thin vi mi trng.
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