thermostressine

No signs of stress in skin

Prevents skin
from jet-lag

anti-stress

protection

Protects cells
against daily stress

Boosts
HSP70 levels

Description

Science

Tetrapeptide that increases HSP70 (Heat Shock

Protein) levels in skin providing a protective shield,
that confers cells a stress tolerance against everyday
challenges and stressful stimuli. It helps skin to be
more relaxed with a more vital appearance.

Stress is a very common situation in todays society

and the skin suffers it as it is the foremost interface
between the environment and the body. Cellular
stress may originate from a variety of stressful situations including environmental (extreme weather,
heavy metals, UV radiation, pollution), physiological (oxidative injury, metabolic and osmotic
stress, lack of sleep, jet-lag) or psychological (daily
stress and emotional shocks) stimuli.
Proteotoxic stress within the cell, which is the
intracellular accumulation of abnormally folded
proteins, can also come from transcriptional and
translational errors together with a decreased
capacity of the proteasome. Responses to stress
are diminished with aging.
thermostressine stimulates HSP70 synthesis and
therefore Hsp72, which is the inducible form of
the family. An increase in this protein enhances
stress tolerance to the skin. Proteotoxic stress can
be modulated and cellular damage prevented,
especially when the skin is repeatedly challenged
or when aging impairs the stress response of the
skin.

Appearance
Transparent solution containing 0.05% active
ingredient.

INCI
Glycerin, Water (Aqua), Acetyl Tetrapeptide-22.
Preservative free.

Properties
HSP may play a role in aging as a defense mechanism
to maintain protein homeostasis. thermostressine
helps cells to fight stress and increasing HSP70
levels also prevents damage from further stress
exposure.

Applications
thermostressine can be incorporated in cosmetic
formulations such as emulsions, gels, serums to
fight stress and treat fragile, tired and dull skin.

Dosage 2-5%

thermostressine

Solubility
Water soluble.

www.lipotec.com

thermostressine

In vitro efficacy

1. HSP70 EXPRESSION WITHOUT PRIOR STRESS

thermostressine

The ability of
to induce the expression of HSP70 in human keratinocytes
was determined by an ELISA assay. A thermal shock (44 C, 30 minutes) and incubation with
the proteasome inhibitor MG-132 were used as positive controls.

2. CELL PROTECTION AGAINST STRESSFUL STIMULI

Thermal shock
a) HSP70 induction
Human primary keratinocytes were incubated with 0.2mM thermostressine and
submitted to a 44 C heat shock for 30 minutes. Immunocytochemistry was performed
to evaluate HSP70 stimulation.

Control cells

Cells + thermostressine
+ heat shock

Cells + heat shock

b) Cell viability

Cells were incubated with thermostressine at different concentrations. Then, human

keratinocytes were exposed either to a heat shock (45 C, 2 hours) or a cold shock
(-20 C, 40 minutes). Cell viability was determined by the Neutral Red Uptake method.
HEAT SHOCK

COLD SHOCK

thermostressine
induced HSP70
expression to a
significant extent (+28%)
without prior stress

HSP70 are increased

after a heat shock,
but stimulation is
higher in presence of
thermostressine

Keratinocytes protection
against heat and cold
shocks is enhanced
when treated with
thermostressine

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Cell viability (%)

+32%

+21%

CT

Cell viability (%)

+65%

UVB-induced damage

+39%
+19%

Cell viability (%)

The photoprotection efficacy of

thermostressine was assessed
in human keratinocytes after
UVB irradiation. Cell viability
was determined by the MTT
method.

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reaching levels similar to

non UVB-irradiated cells.

Dehydration stress

thermostressine
increased cell viability
up to 100%,

thermostressine

After dehydration, cell

viability and cell size
were maintained in
keratinocytes treated with
thermostressine.

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Mean cell area (m2)

The capacity of
to maintain viability and size of skin cells exposed to a
dehydration stress was determined by the Neutral Red Uptake method and cell area
measurements. Human keratinocytes (A0) were incubated with thermostressine and then
introduced in a desiccator for 20 minutes. Cells were left to recover for 24 hours under
standard culture conditions (A24).

thermostressine
prevents cell desiccation

While the claims and supporting data provided in this publication are believed to be reliable and they are presented free and for guidance only, there are no warranties of any kind. All express and
implied warranties are disclaimed. The recipient is solely responsible for ensuring that products marketed to consumers comply with all relevant laws and regulation. LIPOTEC is the exclusive holder
of the both industrial and intellectual property rights identified herein. Recipient of this publication agrees to indemnify and hold harmless each entity of the LIPOTEC organization for any and all
regulatory action arising from recipients use of any claims or information in this publication, including, but not limited to, use in advertising and finished product label claims, and not present this
publication as evidence of finished product claim substantiation to any regulatory authority.

Isaac Peral 17 Pol. Industrial Cam Ral - 08850 Gav (Barcelona) Spain-Tel +34 93 638 80 00 / Fax +34 93 638 93 93 - commercial@lipotec.com

Ed. 10B