Pee Build Better Industrial Bioreactors were fatever configuration for A typical bioresctor has the features ae ioesstore an engineer shown fa Figure The fect veel Here are some ne. proper merical povided wih vera ih las, and ‘guidelines for terial selection, Side pos or pH, temperate. an di ; ncdfshes are rca A we wil Gacus,aalved oxygen sensor ares raininie mechanical \ecp vessel intemal parts 4 minimum, requirement. A stean-sterlizale sample design, materials “lt me! mara foe strizatea and, ale pies. Connestons to eid sad Som cing woe odo: ah (pena ry ge Selection and Shriya: ttc rath alent eo aoe mire) sprgesupolies oxy and * Reactor design Tap wie cule & haven woes Indus rea fr sere operation acted tthe lowes ot onthe eto ace ually designed te presure esels, vse When sechanilsgaon sed feenducumed mor fly cawher(i} enseaneaet oc and tis a ¥. hist, Uns tn ‘Fer Some common arte oft. 3 CHEMEAL EEERNG ORES © LAMY 0 © 55MATERIALS “he bottom placement of sn aptaor nd save cles Up the top of the boractr foe a'spinaing oe vibrating fell retention mes hich ‘Smee ae nama cll store borne The sf Figure 2 proved with Site of double mechanical Seah the pnt of ety 0 {sable double seals with peered oy bene sry gure when i ardour materia) mest be mouse etatr shat: how ‘ron, eafess magnetically api digs me ctiely Spero when loge int ‘ons pet "Te rear is ihe rs pistes ae sommealy sed on flr vessel ut «comes Mehea ee expense fr larger ots The endplate Sipps rupture ds, at forsensore for example, Foam electode) sd ns ‘er nai pres pln. sigh hs wt igh ‘provid an ean be ine tally leaned by a jet of the tems snow In Figure regued in every spleaton. hee i some spi esd, the ake eae othe seed ie thee a protective shroud. as Shawn in Figure 2, fappiee co she jacket. The [elet and the feator are Provided ith overpressure ows ai ae Ping. burting de on fhe tioreactr. Usually & ‘ype Belo pte Wk dhe se Te ater type dies nc develo ees or racke without complete ‘re, Thos weiy i ese ih pie de, "Te veal bid be aly raining. should have & ‘ther tachment oman ee et te tie of crevies ad ap ant asa woe pockets of Tia nd ss oy {5 igee 24 pel rac) rer vel 2) bet uno {air fc pil entre nd eed yen see ‘ean 18 ps rer) mechan shy tesge of each appureny Iino em atthe gasket ‘ooves ss penn E39 (Gorn gerbes / mr 13 hrrenmaie18) W583 how in Fe pehetcomecon (sampler with nam coer” preter ‘ines common for ono ond Te vessel should have jaa cen) arta (19min 19) Cow interes the derign se le ia nae Sa ci ‘eigen (am eer (29) su gles” Siam iepace (CHP) aces ‘i eta shwa} anda cmnco dopave SHanelace (CIE) oes acne fait a an ser out "Sipe onto thowlé fe sable of S116 psi, In mon instances the Blorestor is oengned fora mak trum allowable working peste oF ‘signed a wisn tall vacua No Govonon factor i allowed 10 Nort Arica, he dvgn confor to ASME, Section Vil Dion f Boller and Preiue Vesel Cae, Other cose may be sccepable Improving vessels ‘The ves i hostel est Ing removable pay al or na ales are Insened into the sews Thong one othe ports on the ead ‘pay bass eset. Ber cover fe ofthe tanker povsble with ste gat spares pipe shoul 0 fe capable of eng CIP a Vales, ‘say coups a hr ingssould be ew, As ft pon out 150 ppm fr continuous bles welded Jott should be ‘xpoure of Type 31. peu ouplngs. Sum connections 2 gould oscar during sri ‘Sou ow foreomplee Sa ton, Careful evaluation of the Fecomet ofall i pol fa toda exten ad or ome fie veel. Even he Exe of Bioreactor applieations Type the Director pant shoud be MT or oer igh allay rae ‘lean designed ith smooth ‘uch "6 Ma" nay Be nad Somoure,minimun bare ‘aia cn price Tren ano fer, hanna apesion rsh been aco ‘The nish on surfaces hployedup tom len 800 irs. sel, while he es expensive Type which come in conta it the peo ‘Themen soy npn wid in 308 (oe OME) ed forthe ace Cems mater ad, fo sme exten, the ceupings can witnand the noe he sulation shoud, and other the ih om extra sure ect fal sterteaion condions (about nonpadutcomactig surtces The the ability to cleat, saritize, tnd 2+ ant abut 30min. Sone com er of wiles sts! contin sti the borentr and te gen ‘pertion of magnetic dives have reduces chromium cubis formar finish hae impisuce on sabi ‘in‘eeaed by Duc a Teds ton dng wedng ad lowers the and eat ofthe surface, aed (Mechanica sal with silicon pun fo Iter nergranuar coe may have process implications ‘arbiscnbon runing sree are Rosana he wel ‘ekting to roi oe sna ee Frequently apeciied i biorexctor_ "Compared o Type 306, he Type Sahenon ora ‘plcatom, Those sea mara we 316 set sgnicandy more ess: Stuns Sel mechanically po inert unde normally encountered tant to chloride associated crevice ised w «Bagh "arora hes ondifos in bosacas: andthe crrson and piting The sole high teen in we for borectos fr gute frursirae conde to bemfe'n level ange i incre tobe some Une Meroe exanton (Sssing ‘The con ada fon ombintions ae opi Bity by wince of steer Ing propenies All ari of coston wit wane processing. The mails which | ‘contact surfaces must be capa- | | Dever being easly cleaned Shang CIP techn. Sei Fors petri aa | Stes ete prefered mati 620.05 — tf cosurton fr resco = SREREUe “he Bigwacor vee ssl) Fe Gk ron tec the art ad he cove Ne the oa con ‘made in Type SIOL sssiss tows he gots chan (son RCA ENEERNG ROGRESS © sANOR Hn « 57ger and grooves typical of ‘mechanical pola. The ech {Ea potah fs echeved by strats con ofa sandpaper ype mata ‘on meta. The surface finish ay be specified oy grt number, for Example. 240 rit lish, which por squire in of te abrasive us Te higher the pr am fer the smoother fe finish More quantitative meses of (a) and “root mean square tone” (Re wom tat oven 4320 4 polished ‘Sece wi have Reo te ber of 23-0'309m Clery, tment formicrober and other Inieroscopie particles. Hence, “beta mechanical polish lone ‘lesrpalihing sam seers face’ projections arising from ‘echaicl polishing he eel nt tnsch smoother fin Electrol Thing Santiesnty rede he me al uace se, an hence te pres Sctemetal contact aren, The Incase creas pst ‘eel surface ich in protective tions should sate the level of ‘mechanical polish needed prio the eeciropolah stop. Seanning tlecvon miropaps f electopel lie avantoge wo tating wih uch eter tan 220 gt Raabe {405m policed sure. ‘sed it shouldbe a est 240 fit, and the diecton of polish Shout be eenrlled te produce ‘erica gain for good Srinage ‘The marae sos receve a nih ‘eld wih ceament ar sini ‘The alent of gran does not seem tbe of consequence the frie is tobe clectopolsed LUTERATURE CITED. Blecropoiing removes surace imp the stacey vl. ‘op such laws ay cavities and grooves from where the imprity fas ben solved away Fr spe. flor arface wih fw as pre aloes stelsbould be used nnancton Than, Type 36 fom Consumable electrode or eee agen pce rere of the biotescor shuld be sound Shah withthe tral race, snd Polished othe sane way ner Grikevesel Wels ae fit ce high guy tose (Other materials [Ap rom ils tel, other miele reused ine abi tt teats. Boresicate gas = {ed or sight glases shows i Figure 2 clstomere sch asl ‘one rubber and EPDM (epee Propylene diene monomer) ze ued {ovmabe gushes Pasicy such a PIPE (plycraleoroeyene) fread tan eles ese, Sc moor fering Ceramics Sch ar sitcom earbige ae ‘mpoye fr techies eas fn sap for sels and fire dies. Careful thought shouldbe given tothe spec Experioce of pte vendor, Tamir with be fequivermens ct biophermaceueal produc: {om wef Not al EPDM Buses, for example. say be ‘table ge for Nope Squipment. These terials sional condton an they shoal Sor rlee chemi ltt Mad Don't lose sight of the entre process ontction, ou tus emphasize prope teal selection ad iis Erba you will ave to combine bit more paces specie amet of Deen espn ese geomet Fydrodynamtes: mining. heat tnd ‘eo afer foaming hea fos Iu sie veatercoagunon.