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data. This study will provide more acceptable options for those who are currently at risk or have
a history of heart disease to nutritionally improve their health.
Keywords: vegetarian, protein, cardiovascular disease, and tofu
and acceptable ways to decrease intake of saturated fats, cholesterol and overall calories, to
promote heart health and decrease risk of heart disease. This project will involve the comparison
of two red beans and rice recipes. All ingredients and quantities will remain the same except one
will contain pork sausage and the other will contain a tofu sausage. The samples will be
evaluated based upon their appearance, aroma, taste, and texture. This will be important to help
consumers to see that food does not have to contain meat to be complete from a nutritional
standpoint or satisfactory from a sensory perspective.
Review of Literature
Sensory Evaluation/Experimental Food Chemistry
Sensory evaluation experiments are one way for researchers to determine consumer
acceptance of changes made to a recipe. These experiments are designed for participants to
evaluate the quality of a food item using their five senses (Brown, 2011). One method of
evaluation is through the use of a taste panel, a variety of individuals who will taste controlled
samples of food and evaluate them accordingly (Brown, 2011, p.25). Typically, multiple
samples of a recipe are made, each with one element changed. Variations of the original recipe
could relate to a specific nutrient or ingredient such as sugar or gluten. These changes could be
made in order to improve the health aspects of the food or improve the sensory characteristics of
the dish. This sensory evaluation experiment will be seeking to improve the health aspects. The
independent and dependent variables are shown below.
Table
1
_________________________________________________________________________________________________________
Definitions
of
Variables
Variable
Definition
IV:
Andouille
Pork
Sausage
Meat
from
a
pig
that
has
been
ground
and
blended
with
Cajun
seasoning
and
spices
and
shaped.
IV:
Italian
Tofu
Sausage
Tofu
is
made
from
soy
bean
curd
(McIlveen,
1999).
Seasoned
with
classic
Italian
spices
and
shaped.
DV:
Appearance
Sensory
awareness
of
color,
outward
texture,
size,
and
shape.
Ideal
for
this
recipe
will
be
different
ingredients
well
blended
yet
identifiable.
DV:
Aroma
Sensory
awareness
and
identification
of
the
smell
or
odor
of
food.
Typically
can
fit
in
one
of
six
categories:
spicy,
flowery,
fruity,
resinous,
burnt,
and
foul.
Ideal
aroma
for
this
recipe
will
be
spicy.
DV:
Taste
Sensory
awareness
and
identification
of
sweet,
salty,
bitter,
sour,
and
savory
as
it
relates
to
food
placed
on
the
tongue.
Ideal
taste
for
this
recipe
will
be
savory.
DV:
Texture
The
combination
of
what
the
eye
sees,
the
touch
of
the
fingers
or
eating
utensils,
and
the
mouthfeel
of
the
food.
Textures
felt
in
the
mouth
are
coarse,
crisp,
tender,
fine,
dry,
moist,
greasy,
smooth,
lumpy,
rough,
sticky,
solid,
porous,
bubbly,
or
flat.
Ideal
for
this
recipe
will
be
moist
and
lumpy.
Table 1 illustrates the independent and dependent variables for this experiment. The independent
variables will be Andouille
pork sausage and Italian
tofu sausage. The dependent variable will
be the change in taste, texture, appearance, and aroma. (Brown,
2011).
Current Research
The thought of decreasing meat consumption to improve health is not new. However, it
is not without critics. People may not know that protein can come from sources other than meat
and are unaware of the number of negative elements of meat. There is some research to support
the idea that limiting meat consumption could improve health, specifically decrease risk for
cardiovascular disease, but not extensive amounts. Meat is a huge contributor to the intake of
saturated fat and cholesterol in the American diet, so if its consumption is reduced, logically that
would reduce the levels of saturated fat and cholesterol, and could contribute to lowering obesity
rates and blood pressure, all risk factors for heart disease. The primary issue consumers see with
limiting or eliminating meat is the protein that is removed from the diet. Therefore, often it is
assumed to be an unhealthy choice, however if done correctly it could be found to have
incredible benefits. Table 2 illustrates a summary of six article discussing different aspects of
this issue.
Table
2
Summary
of
Studies
Linking
Heart
Disease
and
Meat
Consumption
Publication
date,
Author(s)
Sample
n
Measures
Findings
Journal
2007,
Harvard
n/a
n/a
parts
of
a
Do
not
leave
out
protein.
Health
vegetarian
Remember
on
non-starchy
Commentaries
meal
vegetables.
Whole
grains
are
important.
Variety
is
key.
Look
for
healthy
fats.
May
need
to
make
extra
effort
to
get
adequate
calcium,
iron,
and
vitamin
B12.
2013,
Journal
Rizzo,
N.
n=71,751
nutrient
Distinct
diet
patterns
of
the
Academy
Jaceldo-Siegl,
K.
intakes
of
causes
some
major
Sabate,
J.
Fraser,
G.
Tester,
J.
James,
D.
vegetarians
and
non-
vegetarians
differences
in
nutrient
intakes.
Nonvegetarians
had
the
lowest
intake
of
plant
and
plant
elements,
while
having
the
highest
intake
of
saturated
fat,
and
highest
BMI.
Strict
vegetarians
were
the
reverse.
Energy
intake
was
about
the
same.
n=311
meta-
analysis
n/a
properties
of
soy
sensory
Tofu
was
not
acceptable
evaluation
in
regard
to
taste
and
of
meat
texture,
but
was
for
substitutes
appearance.
Quorn
did
not
have
a
good
appearance
but
taste
and
texture
were
very
comparable
to
the
real
meat.
meta-
All
of
the
main
contributors
analysis
to
heart
disease,
cholesterol
of
research
obesity,
and
blood
pressure
done
on
are
positively
effected
by
a
vegetarian
vegetarian
diet.
Healthy
fats
diets
and
increase
and
saturated
and
heart
disease
trans
fat
decrease.
Other
healthy
macro
and
micro
nutrients
are
found
in
McIlveen,
H.
n=12
Abraham,
C.
Armstrong,
G.
Whitten,
C.
n/a
higher
amounts
in
vegetarians.
Table
2
summarizes
the
six
studies
in
which
were
examined
to
see
the
results
of
a
vegetarian
diet
on
risk
for
heart
disease.
The
sample
sizes
ranged
from
n
=
12
to
n
=
71,751.
The
results
show
promise
for
meatless
meals
because
all
articles
demonstrated
improvements
in
heart
health
due
to
less
meat,
or
plant
protein
substitutes.
The key to a healthy vegetarian meal is getting adequate protein from another source.
Choosing to leave the protein out may contribute to overeating, because protein plays a huge role
in the feeling of fullness after a meal (OMeara, 2009). Some examples of plant sources of
protein include hummus (made from chickpeas), beans, nuts, seeds, nut butters, and soy products
(OMeara, 2009). Since most plant proteins are not complete proteins like animal products,
variety is important in order to get all of the essential amino acids the body needs. Variety also
helps keep meals interesting; consumers who are accustomed to eating meat at every meal could
add a few meat-free meals into the mix for some variety (OMeara, 2009).
Meatless meals can be more than adequate nutritionally, they can be superior. One
experiment studied specific nutrient intake levels in participants grouped by varying levels of
vegetarian eating habits, ranging from nonvegetarians to strict vegetarians (Rizzo, 2013). This
study found that strict vegetarians had the lowest average BMI at 24.0 and the numbers increased
steadily as meat consumption increased in the different categories all the way up to the highest
average BMI of 28.7 in the nonvegetarian group (Rizzo, 2013). The strict vegetarian group also
had the smallest percentage of participants who were obese, 9.4%, while the nonvegetarian group
had the highest percentage at 33.3% (Rizzo, 2013). These results show a definite association
between the increased consumption of animal products with higher rates of obesity and higher
BMI levels (Rizzo, 2013). This study also examined the intake of saturated fat among the groups
and found the strict vegetarian group to have the lowest intake (Rizzo, 2013). It is also
interesting to note that the percent of energy intake from protein was about the same for each
group, 14.9% for nonvegetarian to 14.1% for strict vegetarian (Rizzo, 2013).
Research has also found that a vegetarian diet can lower blood pressure and cholesterol
levels. Tester (2014) did a meta-analysis of seven clinical studies and thirty-two observational
studies to determine whether or not a vegetarian diet had a significant effect on lowering blood
pressure. Both the clinical and observational studies showed that the vegetarian diet, compared
to the omnivorous diets, provide a noteworthy contribution to lowered blood pressure (Tester,
2014). The reduction in blood pressure could be due to a variety of aspects of a vegetarian diet
such as generally lower obesity rates, less salt consumption, more potassium, fat consumption
consisting of proportionally more polyunsaturated fat than saturated fat, and finally more
vegetable proteins (Tester, 2014).
Another meta-analysis in 1995 looked at all of the primary risk factors for cardiovascular
disease and how a vegetarian diet affects each of those elements. This study found the average
levels of total cholesterol, LDL cholesterol, and triglycerides in the blood to be lower in
vegetarians than omnivores (Whitten, 1995). They also discovered, based upon one specific
study, that there was an association between how strict the vegetarian diet was and cholesterol
levels (Whitten, 1995). Whitten (1995) also found, in another study, a connection between an
increase in cholesterol levels and even limited meat consumption and seemingly insignificant
increases in fat intake. There is a parallel here to the way higher average BMI levels were
connected to increased meat consumption in Rizzos study (2013).
10
Soy products are especially good meat replacements because they provide a more heart
friendly source of complete proteins. Soybeans provide many great things for health without the
negative nutrients. They are one of the few plant sources of omega-3 fatty acids, fiber, B
vitamins, and several other important minerals (James, 2004). Isoflavones, a phytochemical that
is naturally present in plants, and soy protein are elements that could contribute to helping blood
vessels remain flexible and avoid blood clots (James, 2004). On paper, soy is a perfect meat
substitute. However, in a practical realm, when discussing food that consumers must choose to
eat there are sensory elements to take into account. A sensory evaluation experiment done in
1999 compared meat products with plant-based, protein rich substitutes, one of which was tofu, a
soy product. (McIlveen, 1999). The tofu scored well in comparison with the meat in the
categories of aroma and appearance, however did poorly for flavor and texture (McIlveen, 1999).
Tofu does not have much flavor of its own and as a result probably should not be used as a meat
substitute (McIlveen, 1999). Instead, tofu would be better used and potentially better accepted as
just a protein source in a recipe rather than a stand-alone meat replacement like a chicken breast
(McIlveen, 1999). This proposed experiment would be a step in this direction of determining
how well tofu is accepted in a recipe where is can absorb flavors around it and be a part of the
dish as a whole.
Disease State
High cholesterol levels, hypertension, and obesity are all risk factors for cardiovascular
disease (National Heart, 2014). Research has shown, in several studies and experiments, that a
vegetarian diet or decreasing meat consumption can contribute to decreasing the prevalence of
these risk factors. More research needs to be done to provide consistent, solid evidence on this
issue. Studies done twenty, thirty, and even forty years ago have found that subjects who
11
The purpose of this study is to evaluate consumer acceptance of Andouille
tofu
sausage
instead
of
Andouille
pork
sausage
in
two
identical
red
beans
and
rice
recipes.
The
dishes
will
be
prepared
using
identical
procedures
and
equipment,
but
the
sausage
used
will
be
different.
Participants
will
then
be
asked
to
evaluate
the
two
dishes
based
upon
their
taste,
texture,
appearance,
and
aroma.
This
experiment
will
be
significant
to
anyone
seeking
to
promote
and
implement
heart
healthy
dietary
practices
in
either
prevention
or
correction.
Methods
Participants
The
participants
for
this
experiment
will
include
about
thirty
senior
nutrition
students
and
three
or
four
nutrition
faculty
members.
The
ages
of
the
students
will
range
from
twenty
to
about
thirty-five
and
the
ages
of
the
faculty
members
will
vary.
All
students
and
faculty
will
be
from
a
public
university
in
the
mid-south
region
of
the
country.
All
students
will
be
enrolled
in
the
Experimental
Food
Science
class
and
participation
in
this
experiment
will
be
required.
Participants
will
come
from
a
variety
of
socioeconomic
statuses
and
will
include
a
variety
of
ethnicities.
12
Research
Design
This
experiment
has
been
designed
to
measure
consumer
acceptance
of
tofu
sausage
in
red
beans
and
rice
instead
of
pork
sausage.
The
control
recipe
(Appendix
A)
will
use
smoked
pork
sausage
as
the
independent
variable
and
the
modified
recipe
(Appendix
B)
will
replace
the
pork
sausage
with
Italian
tofu
sausage
as
the
independent
variable.
The
participants
will
then
evaluate
each
recipe
in
regards
to
taste,
texture,
appearance,
and
aroma.
These
sensory
aspects
will
serve
as
the
dependent
variables.
The
participants
responses
will
help
determine
the
level
of
acceptance
of
the
vegetarian
version
of
the
control
recipe.
Measures/Instrumentation
The
experiment
will
be
done
in
the
Food
Science
lab,
room
214
of
McAlister
Hall
on
the
campus
of
the
University
of
Central
Arkansas.
The
equipment
needed
to
prepare
the
food
for
this
study
will
be:
an
electric
stovetop,
large
metal
pot,
serrated
knife,
straight
edge
knife,
plastic
cutting
board,
liquid
measuring
cup,
one
tablespoon
measuring
spoon,
can
opener,
one
cup
dry
measuring
cup,
and
a
wooden
spoon.
The
samples
will
be
placed
before
the
participants
in
identical
small
bowls
labeled
on
the
bottom
with
a
number.
The
number
192
will
indicate
the
recipe
with
pork
sausage
and
the
number
586
will
indicate
the
recipe
with
the
tofu
sausage.
The
samples
will
be
given
to
the
participants
at
the
same
time
and
a
glass
of
water
will
be
provided
to
cleanse
their
palate.
Evaluation
of
the
samples
will
be
done
using
a
scorecard
(Appendix
C)
with
a
nine-
point
Hedonic
scale
to
rate
the
appearance,
aroma,
taste
and
texture
of
the
individual
samples.
Next
to
each
sensory
aspect
participants
will
be
asked
to
write
a
number
that
reflects
their
acceptance
of
that
element.
The
scale
will
be
from
one
to
nine,
one
13
representing,
dislike
extremely,
and
nine
representing,
like
extremely.
A
comments
box
will
be
available
at
the
bottom
of
the
scorecard
for
any
addition
thoughts
the
participants
may
have.
The
data
will
be
collected
and
analyzed
by
looking
at
the
mean,
median,
and
mode.
These
values
will
be
put
in
the
form
of
a
bar
graph
to
visually
represent
them.
Procedure
The lab will be set up before the participants enter the room. Each seat will have
instructions,
a
scorecard
with
a
number
in
the
top
right
hand
corner,
the
subjects
identification
number,
a
pencil,
a
glass
of
water,
a
spoon,
fork,
and
napkin.
The
seats
will
be
set
up
in
a
way
that
allows
privacy
for
the
participants;
they
will
not
be
able
to
see
each
other
during
the
study.
The
exact
procedure
is
as
follows:
1. Participants
will
then
enter
the
room
and
choose
a
seat.
2. The
instructions
will
be
read
aloud.
3.
The
participants
will
be
asked
if
they
have
any
food
allergies.
4. Any
necessary
adjustments
will
be
made
before
proceeding.
5. The
experiment
will
now
begin.
6. Both
samples
will
be
brought
to
the
participants
at
the
same
time.
They
will
be
labeled
on
the
bottom
with
an
identifying
number.
7. The
participants
will
taste
one
sample
and
then
fill
out
that
portion
of
their
scorecard
before
proceeding
to
taste
the
next
one.
8. In
between
samples
they
will
drink
some
water
to
cleanse
their
palate.
9. They
will
repeat
the
same
process
for
the
second
sample.
10. Once
a
subject
has
completed
their
scorecard
they
are
free
to
leave.
14
11. They
will
leave
everything
at
their
seat
and
the
researcher
will
come
by
and
pick
up
their
scorecard
and
left
over
supplies.
Data
Collection
&
Analysis
Plan
Once
all
participants
have
completed
the
experiment,
the
data
will
be
gathered
from
the
participants
scorecards
and
entered
into
an
Excel
spreadsheet.
Data
will
be
connected
to
each
participant
by
their
given
number.
From
there
a
bar
graph
will
be
created
to
visually
display
the
level
of
acceptance
of
each
element.
The
x-axis
of
the
graph
will
be
the
dependent
variables,
appearance,
aroma,
taste,
and
texture.
The
y-axis
will
be
the
level
of
acceptance
in
accordance
with
the
nine-point
Hedonic
scale.
For
each
of
the
dependent
variables
there
will
be
two
different
colored
vertical
bars.
One
bar
will
be
orange
and
will
represent
the
control
recipe
with
pork
sausage.
The
other
bar
will
be
blue
and
will
represent
the
modified
recipe
with
tofu
sausage.
From
the
graph
it
will
be
easy
to
see
how
consumers
rated
each
recipe.
Ethical
Consideration
During
this
experiment
participants
will
be
allowed
to
withdraw
at
anytime.
Their
participations
is
encouraged
but
not
required.
No
personal
information
will
be
recorded
from
the
participants
and
this
experiment
presents
no
foreseeable
harm
to
them.
Because
this
experiment
falls
within
the
scope
of
a
university
course
approval
from
the
Institutional
Review
Board
is
not
necessary.
Food
allergies
will
be
discussed
prior
to
the
experiment
beginning
and
participants
with
a
related
food
allergy
will
be
exempt.
15
protein
that
can
be
substituted
for
meats.
This
will
be
significant
for
those
already
at
risk
for
heart
disease
as
well
as
those
seeking
to
improve
their
general
health.
It
will
also
be
beneficial
for
food
manufactures
to
know
what
aspects
of
plant
protein
are
most
acceptable
to
consumers.
Weaknesses
or
Limitations
This
study
investigates
consumer
acceptance
of
tofu
sausage
instead
of
pork
sausage.
However,
the
consumers
used
are
from
a
small
and
convenient
sample.
This
is
a
limitation
for
the
experiment,
as
it
does
not
allow
for
the
findings
to
be
generalized
for
other
populations.
This
study
would
need
to
be
replicated
with
a
more
diverse
and
larger
sample
of
subjects
to
strengthen
the
findings.
Internal
and
External
Validity
The internal validity of this experiment is confirmed by the modification of only one
element
of
the
recipe.
From
the
control
sample
to
the
modified
sample
only
the
type
of
sausage
is
different.
Therefore,
any
differences
in
the
responses
to
the
dependent
variables
must
be
due
to
that
change
alone.
A
major
factor
in
external
validity
is
the
ability
for
the
results
to
be
generalized
to
populations
other
than
the
sample.
As
already
mentioned
this
aspect
of
the
study
is
fairly
low.
There
is
not
much
potential
for
generalizing
at
this
point
in
time.
16
Future
Practice
This
study
will
affect
future
practice
by
providing
more
acceptable
vegetarian
alternatives
to
meat,
even
for
those
who
do
not
want
to
go
without
the
experience
of
eating
meat.
Tofu
is
a
great
source
of
protein
and
many
other
micronutrients.
This
experiment
can
offer
health
care
professionals
practical
examples
of
foods
that
are
proven
to
be
acceptable
to
consumers
and
beneficial
to
heart
health.
17
Center for Disease Control and Prevention. (2014, July 14). Leading causes of death. Retrieved
from http://www.cdc.gov/nchs/fastats/leading-causes-of-death.htm
James, Delores C.S., ed. "Soy." Nutrition & Well-Being A to Z. New York: MacMillan
Reference, 2004. Gale Cengage Learning. Web.
McIlveen, H., Abraham, C., & Armstrong, G. (1999). Meat avoidance and the role of replacers.
Nutrition and Food Science, 99(1), 29-36. Retrieved from http://0search.proquest.com.ucark.uca.edu/docview/217625328?accountid=10017
National Heart, Lung, and Blood Institute. (2014, July 10). Lower heart disease risk. Retrieved
from http://www.nhlbi.nih.gov/health/educational/hearttruth/lower-risk/risk-factors.htm
OMeara, M. (2009). Balancing a vegetarian meal. Harvard Health Commentaries. Retrieved
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19
Ingredient
Directions
1 Tbsp
Vegetable Oil
1 lb
pieces
1 small
Onion, chopped
1 medium
1 stalk
Celery, chopped
2 cloves
Garlic
2 cups
Vegetable broth
2 cups
a simmer.
tsp
Creole seasoning
20
Carbohydrates
Fat
Protein
Sodium
Sugar
576
52g
29g
27g
1,939mg
4g
21
Ingredient
Directions
1 Tbsp
Vegetable Oil
1 lb
Tofurky Italian sausage, sliced into Add the sliced sausage and cook for
in. pieces
1 small
Onion, chopped
1 medium
1 rib
Celery, chopped
2 cloves
Garlic
2 cups
Vegetable broth
2 cups
Instant rice
a simmer.
tsp
Creole seasoning
22
Carbohydrates Fat
Protein
Sodium
Sugar
469
56g
30g
1,219mg
6g
14g
23
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