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.. / .. //
. .. . , . 2011. . 24 (63), 2. . 3-21.
,
. .. , 2011 50- .
: . .. ,
.
Chuyan E.N. History of the department of physiology and animal rightsand biophysics / E.N. Chuyan // Scientific
Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 3-21.
Article is devoted to the history of development, development of the department of human physiology and
biophysics and animals Tauride National University. V.I Vernadsky, which in 2011 celebrates its 50th anniversary.
Keywords: Taurian National University. V.I Vernadsky, Department of Human and Animal Physiology and Biophysics.

17.06.2011 .

21

. . .
, . 24 (63). 2011. 2. . 22-29.

4777.75


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E-mail: timur328@gmail.com
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. 2010
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35. ..
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36. ..
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37. ..
( ,
). 2011 .
- . ..
' .. / .. ' //
. .. . , . 2011. . 24 (63), 2.
. 22-29.
,
. .. 50 .

.
: , ,
.
Temuryants N.A. Crimean school magnetobiology / N.A. Temuryants // Scientific Notes of Taurida
V.I. Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 22-29.
Provides information about magnetobiological studies conducted at the Department of Human and Animal
Physiology and Biophysics of Taurida V.I. Vernadsky National University in the last 50 years. These studies
relate to the study of biological effects of low-intensity electromagnetic fields and very low microwave
frequencies and biorhythm.
Keywords: low-intensity electromagnetic fields and very low microwave frequencies, biorhythm, Department
of Human and Animal Physiology and Biophysics.

14.05.2011 .

29


. . .
, . 24 (63). 2011. 2. . 30-49.

612.135:528.811+537-96





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4.

A Manual of Acarology/ [edited by G.W.Krantz, D.E.Walter]. Texas Tech University Press, 2009.
595 p.
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5.
6.

7.
8.
9.
10.
11.
12.
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15.
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17.
18.
19.
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21.
22.
23.
24.
25.
26.

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/ .. , .. //
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/ .. , .. // : 75 : . .
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.. : / .. .,
2001. 22 .
.. / .. //
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.. / .., .., .. // .: .
1984. 456 .
.. / .., .., .. // .:
. 1975. 213 .
..
/ .. //
. 1996. 5. . 24.
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/ .. , .. , .. [ .] //
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..
/ .. , .. , .. //
. 2000. . 40, 2. . 14-17.
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27. .. / .. // .: . - 1990. 352 .
28. .. / .. ,
.. // .. . 1990. .109. 5. .420 422.
29. .. // .. //
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31. ..
: . . . . .
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32. .. /
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33. .. / .. , .. . -.: .
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[et al.] // Microvascular Research. 2003. Vol.65. P. 160171.
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myogenic and neurogenic vasoconstriction as well as arterial and venous pressure fluctuations /
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36. .. (
) / .. .: , 2003 328 .
37. ..
/ . . // . 2006. . 32, 5. . 95.
38. .. (
-): : 14.00.01 /
. , 2000 . 128 .
39. .. - -
: . . .
. . : 14.00.34 / .. // , 1996. 25 .
40. ..
/
.. // . 1997. . 9. . 1824.
..
i
/ .. , .. //
. .. . , . 2011. . 24 (63), 2.
. 30-49.
i ()
i 18-23
. ,
( ), () ii
-, i -
,
. ,

- i
i, .

48


: ,
, i, , -, -,
i .
Ananchenko M.N. Skin microcirculation in the functional load of the subjects with different typological
characteristics under the influence of low intensity millimeter radiation / M.N. Ananchenko,
E.N. Chuyan // Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry.
2011. Vol. 24 (63), No 2. . 30-49.
The method of laser doppler flowmetry (LDF) revealed certain differences in the mechanisms of regulation of
tissue blood flow in apparently healthy female aged 18 to 23 years, with different
types. It is shown, that in the mechanisms of the action of low-intensity electromagnetic radiation of extremely
high-frequency (EMR EHF), or millimeter range (mm) leads on microblood circulation human skin play the
main role endothelium-dependent, myogenic endothelium-independent and neurogenic components of the
regulation of tissue blood flow, but their expression depends on the typological peculiarities of the
microcirculation processes subjects. Thus, course impact of EMR EHF leads to leveling of intergroup
differences and approximation values of the functional tests in subjects with hypo- and hyperemic types to the
most balanced in the functional relation normoemical, that testifies to its homeostatic action.
Keywords: low-intensity electromagnetic radiation of millimeter range, the microcirculation of the blood, the
method of laser Doppler flowmetry, functional tests, hypoemical, hyperemic, normoemical types of
microcirculation.

14.05.2011 .

49

. . .
, . 24 (63). 2011. 2. . 50-61.

579.62+615.33+599.537


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.

1.
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3.
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8.
9.
10.
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19.
20.
21.

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: .. ., .. ,
. ., 2 5 . 1990 .: . ., 1990. . 6566.
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, 1999. 400 .

. ., 1983. 15 .
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.. , .. , .. // . 2007.
. 6. . 141147.
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2008. 64 .
. 2001/2002. .: , 2000. 1102 .
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/ [ .., .., ..,
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2004. 528 .

60


22. .. / .. , .. //
. - , 2003. 1008 .
..
(Tursiops truncatus), / .. ,
.. , .. //
. .. . , . 2011. . 24 (63), 2. . 50-61.
,
.
.
.
: , , .
Andreeva N.A. Antibiotic sensitivity of opportunistic pathogenic microflora of bottlenose dolphins
(Tursiops truncatus) kept in Oceanarium conditions / N.A. Andreeva, T.V. Ostapchuk, O.V. Liskun //
Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry. 2011.
Vol. 24 (63), No 2. . 50-61.
The results of long-term research on antibiotic sensitivity of microflora of dolphins kept in Oceanarium
conditions are given here. High sensitivity of opportunistic pathogenic microorganisms to cyclosporines has
been revealed. The obtained results make it possible to choose an optimal medicine and develop individual
tactics of antibiotic therapy.
Keywords: sensitivity to antibiotics, opportunistic pathogenic microflora, bottlenose dolphin.

26.05.2011 .

61

. . .
, . 24 (63). 2011. 2. . 62-66.

613.644




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Balichiyeva D.V. Study of protein metabolism in the body of experimental animals exposed to physical
environment factors / D.V. Balichiyeva // Scientific Notes of Taurida V.I. Vernadsky National University.
Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 62-66.
The article presents data on the effect of vibration on protein and amino acid metabolism in the body of
experimental animals exposed to general vibration. Disturbances in the metabolism of proteins and amino acid
metabolism associated with shifts in nitrogen metabolism during exposure.
Keywords: vibration, protein metabolism, amino acid.

16.05.2011 .

66

. . .
, . 24 (63). 2011. 2. . 67-72.

504.42:579.26 (262.5)


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: , , .
Belyaeva O.I. Abundance and biomass of bacteria in the district of receipt of thundershower flow in a
Kazachya Bay (Black sea) / O.I. Belyaeva // Scientific Notes of Taurida V.I. Vernadsky National University.
Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 67-72.
Data on determination of abundance and biomass of bacteria in the district of receipt of thundershower flows
in a Kazachya Bay werw cited. The off-shore area of bay of receipt was the most eutrophical. Two peaks of
abundance of bacteria are registered: maximal in spring and less expressed - in autumn. The highest general
abundance of bacteria was observed in a winter period at the mass accumulation of jelly-fishes Aurelia aurit.
Keywords: bacterioplankton, thundershower flows, Black sea.

13.05.2011 .

72

. . .
, . 24 (63). 2011. 2. . 73-82.

634.8:631.532/.535

IN VITRO
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// . 2004. . 41. . 4553.

.. in vitro / .. , .. - //
. .. . , .
2011. . 24 (63), 2. . 73-82.
,
, .
, (0,5 /) (1,0
/).
. TERAWET
in vivo.
: , , , ,
, .
Bugaenko L. The grapevine morphogenesis of in vitro culture / L. Bugaenko, L. Ivanova-Khanina //
Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry. 2011.
Vol. 24 (63), No 2. . 73-82.
Shown the necessity to consider a genotype and features initial buds to choose a nutrient medium for apical
meristems cultivation. The nutrient medium containing BAP (0,5 ml/l) and GA (1,0 ml/l) is the optimum for
cultivation grapes microsprouts. Revealed that investigated genotypes have different reaction to concentration
IAA for stimulation rhizogenesis. Established a high effectiveness of using the absorbent TERAWET for
adaptation to in vivo conditions.
Keywords: clonal micropropagation, grapes, explants, culture of the apical meristems, a nutrient medium,
micrografts.

16.05.2011 .

82

. . .
, . 24 (63). 2011. 2. . 83-90.

: 612.2 + 612.014.464



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/ . .. 1983. 254 .
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/ .. , .C. // 1996. . 1. . 125132
Crapo R.O. The role of reference values in interpreting lung function tests / R.O. Crapo // Eur. Respir.J.2004. Vol. 24, 3. P.341342.
.. / .. ., 1997. 344 .
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.. . ., 1998. .279245.
9. Guenette J.A. Respiratory mechanics during exercise in endurance-trained men and women /
J.A. Guenette, J.D. Witt, D.C. McKezie // J. Physiol., June 15, 2007. 581(3). . 13091322.
10. / . . . . .2. ., 2004. 567 .
.. / .. //
. .. . , . 2011.
. 24 (63), 2. . 83-90.
.
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Bukova A.V. Respiratory mechanics pecularities in aged women / A.V. Bukova // Scientific Notes of
Taurida V.I. Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2.
. 83-90.
Stuady of respiratory mechanics in aged women was perfomed. Different levels in ventilatory abilities were
revealed. Factors promoted aged changes in respiratory mechanics, which lead to high pulmonary risk were
determined.
Keywords: aged women, respiratory mechanics, pulmonary risk.

14.05.2011 .

90

. . .
, . 24 (63). 2011. 2. . 91-96.

: [612.66+616-092]: 577.15


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Lopez-Torres M., Perez-Campo R., Rojas C. et al. Sensitivity to in vitro lipid peroxidation in liver and
brain of aged rats. // Rev. Esp. Fisiol. 1992. -- Vol.48, N3. P.191--196.
Romeo R.D., McEwen B.C. Stress and the adolescent brain // Ann. N. Y. Acad. Sci. 2006. Vol.1094.
-- P.226--234.
Toledo-Rodriguez M., Sandi C. Stress before puberty exerts a sex- and age-related impact on auditory
and contextual fear conditioning in the rat // Neural Plast. 2007. Vol.2007. -- P.71203.

95

..
4.
5.
6.
7.

.., ..
//
. 2007. 3. . 217.
.., .., .., .. . .
. .: , 2006. 556 .
Protein measurement with the Pholin phenol reagent. / O.H. Lowry, K.J. Rosenbrough, A.L .Farr, K.I.
Rendall. // J.Biol.Chem. - 1955. Vol.193, N1. -- P.265--267.
Svennerholm L., Bostrum K., Helander C.G. et al. Membrane lipids in the aging human brain //
Neurochemistry. 1990. Vol.12. P.547--554.

..
/ .. //
. .. . , . 2011. . 24 (63), 2.
. 91-96.
, 12- 24-
, , 2 , 1,5-
. 1,5-
.
.
: , , , .
Volkova Y.V. Age-specific peculiarities of the change in low-molecular antioxidant level in rat brain and
liver under conditions of immobilization stress / Y.V. Volkova // Scientific Notes of Taurida
V.I. Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 91-96.
It has been shown that immobilization of 12- and 24-month-old rats was not accompanied by a change in urea,
uric acid and bilirubin concentrations in postmitochondrial fraction of liver. In 2-month-old rats the
immobilization was accompanied by an increase in uric acid and bilirubin concentrations, in 1,5-month-old
rats it was accompanied by an increase in uric acid concentration. In brain postmitochondrial fraction of 1,5month-old rats immobilization was accompanied by an increase in urea concentration, while a change in urea
or uric acid concentration wasnt characteristic for the rats of older age groups under conditions of stress.
Keywords: immobilization stress, brain, liver, low-molecular antioxidants.

19.05.2011 .

96

. . .
, . 24 (63). 2011. 2. . 97-106.

615.796.33




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.., .. // . 1996. 12. . 3-15.
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15. .. / .. .: , 1995. 256 .
16. ..
/ .., .., .. .: , 1977. 335.
17. Kaplow L.S. A histochemical procedure for localizing and evaluation leukocyte alkaline phosphatase
activiti in smears of blood and marrow / L.S. Kaplow // Blood. 1955. - 10. P/1023-1029
18. ..
/ .. , .. // . 1996. 2. . 1520.
19. .. / .. , ..
//
. , 1974. .129-138.
20. .. (
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..
/ .. ,
.. , .. //
. .. . , . 2011. . 24 (63), 2. . 97-106.


. ,
,
.
: ,
, , , , .
Grabovskaya E. Role of ultra-high frequency electromagnetic field (UNF EMF) in the functional
correction of the sympathoadrenal system and the types nonspecific adaptation reactions of the
organism in athletes / E. Grabovskaya, E. Nagaeva, M. Nazar // Scientific Notes of Taurida V.I.
Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 97-106.
The work is devoted to the study of ultra-high frequency electromagnetic field (UNF EMF) on the
development of nonspecific adaptation reactions and the content of catecholamines in erythrocytes athletes. It
is shown that under the ultra-high frequency electromagnetic field (UNF EMF) of extremely high frequency
changes the type of nonspecific adaptation reactions of the organism, changing the content of catecholamines
in erytrocytes athletes.
Keywords: ultra-high frequency electromagnetic field, non-specific adaptation reactions of the organism,
erythrocytes, catecholamines, sympathoadrenal system, athletes.

24.05.2011 .

106

. . .
, . 24 (63). 2011. 2. . 107-116.

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.
-
.

1.
2.
3.
4.
5.
6.
7.

Guizouarn H. Cell volume regulation: the role of taurine loss in maintaining membrane potential and cell
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E29-E36.
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20.
21.
22.
23.
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Biol. Chem. 2003. V. 278, 50. P. 50151-50157.
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the glycine receptor / Han N.L., Clements J.D., Lynch J.W. // J. Biol. Chem. - 2004. - V. 279, 19. - P.
19559-19565.
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35. .. / .., .. //
. 2010. . 56, 4. . 111-120.

..
/ .. , . , .. //
. .. . , . 2011. . 24 (63),
2. .107-116

,
. , 1,4 /
15
(0,05 /) 60-
15 , 2 .
: , , , Na+, K+, Ca2+.

Grinchenko .. The influence of taurine on the ion content of gastric juice at the histamine
stimulation of secretion in dogs / .. Grinchenko, V.M. Baban, P.I. Yanchuk // Scientific Notes of
Taurida V.Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 107-116.
The influence of taurine on Na+ and K+ ion concentration in gastric juice stimulated by histamine were
investigated in chronic experiments on dogs with gastric fistulaes. It was shown that taurine in dose 1,4 mg/kg
body weight increased the Na+ concentration in gastric juice at the beginning of experiment at the introduced
15 minutes before histamine injection (0,05 mg/kg) and decreased the K+ concentration during the first 60
minutes of experiment at the introduced both 15 minutes before and 2 hour before stimulator application.
Keywords: taurine, gastric secretion, ion change, Na+, K+, Ca2+.

21.05.2011 .

116

. . .
, . 24 (63). 2011. 2. . 117-123.

001.891:[577.114-021.632:599.323.4]



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333 .
[ ] // http://www.biolavka.kiev.ua/litbadbad.shtml
Mark-Herbert C. / Development and marketing strategies for functional foods / . Mark-Herbert //
Agbioforum . 2003. Vol. 6. P. 7578.

122


4.
5.
6.
7.
8.
9.
10.
11.
12.

13.

.. / / .. , ..
, .. // . - 1999. - 3. [ ]
http://www.diabet.ru/Sdiabet/1999-03/3.htm
.. / ..
// " ". . 2006. 8(34) [ ]
http://www.gastroscan.ru/literature/authors/2025
.. / -
/ .. , .. , .. // .
. - 1996. - .42, 6. - . 3-8.
.. / . .. //
, 2006 [ ] /www.rmj.ru/ articles_857.htm

[
]
http://www.ipharm.sp.ru/farm%20m_gipoglikimiy.htm.
Bonner-Weir S. / Perspective: Postnatal pancreatic cell growth / S. Bonner-Weir // Endocrinology. 2000. - Vol. 141. - 6. - P. 1926-1929
.. - in
vitro [ ] // http://elib.sci.am/2003_1/10/10r.htm.

: . .. ... / // 2-
. . - - 1991. - 16 .
.. /

/ .. .. , .. [ .] // . . - 2006. -
2 (120). - . 171-175.
West I.C. Radicals and Oxidative Stress in Diabetes/ I.C. West // British Diabetic Association. Diabetic
Medicine. - 2000. 17. - P. 171-180.

..
/ .. , .. //
. .. . , . 2011.
. 24 (63), 2. .117-123
()
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: , , ().
Danilova A.O. Study of influence of high-carbonhydrate additions on the state of rats which was
induced by an alloxan / A.O. Danilova, A.V. Zaporozchenko // Scientific Notes of Taurida V.Vernadsky
National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 117-123.
Influence of bioactive additions (BAA) is studied on the basic physiological and biochemical indexes of rats
with a diabetes mellitus which was induced by an alloxan. It is found out that all investigational additions
diminish maintenance of glucose in the serum of blood of rats with alloxan diabetes, thus the prophylactic
feeding of animals by BAA allows to decrease toxic influence of alloxan. The most effective was a BAA with
introduction of probiotic microorganisms, that showed up both in the insignificant increase of basale level of
glucose in the serum of blood and during realization of glucose-tolerant test.
Keywords: bioactive additions (BAA), diabetes was induced by an alloxan, glucose-tolerant test.

13.06.2011 .

123

. . .
, . 24 (63). 2011. 2. . 124-128.

517.121:963




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. .. . 2008. . 21 (60),
2. . 46-49.
..
/ .. , .. , .. [ .] // .
. 1999. . 127, . 1. .18-20..
.. : ,
/ .. // . 2004. . 69, . 1. . 5-7.
.. :
/ .. , ..
, .. [ .] // . 2002. . 67, . 3. . 413-421.
Drabkin D. A simplified technique for large scale crystallization of myoglobin and haemoglobin in the
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N. Ohishi, K. Yogi // Analit. biochem. 1979. - V. 95. P. 351-358.
/ . .. .: ,
1969. . 287-288.
.. / .. .: ,
1980. 271 .
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127

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/ .. , .. , , .. //
. .. . , .
2011. . 24 (63), 2. . 124-128.
, ,
,
( -6--),
.
.
: , , -6--, ,
, .
Yolkina N.M. The enzymatic activity of human erythrocytes under ischemic heart disease in the
conditions of oxidative stress / N.M. Yolkina, S.V. Konoshenko, Ilias Shashua, O.V. Zhugina // Scientific
Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63),
No 2. . 124-128.
It has been shown that under ischemic heart disease in the conditions of oxidative stress the activity of
enzymes in erythrocytes that catalising trigger reactions of glycolysis and pentose phosphate pathway is
rised. At the same time the activity of glutathionereductase is rised also. The changes of activity of some
enzymes in erythrocytes may have the compensatoric significance.
Keywords: erythrocytes, hexokinase, glucose-6-phosphate-dehydrogenase, glutathione-reductase, ischemic
heart disease, oxidative stress.

17.05.2011 .

128

. . .
, . 24 (63). 2011. 2. . 129-135.

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, Q. erris
[21]. Popolus deltoides, Betula verrucosa
Acer saccharinum. P.
deltoides B. verrucosa [2325]. .. .. [19]

Popolus L. (P. deltoids, P. simonii Carr., P. nigra),
[26]. ..
.. [27] P. deltoides Caragana arborescens.

- .
,
,
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.

1.
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, ,
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133

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1.
2.
3.
4.
5.
6.
7.

8.
9.
10.
11.
12.
13.
14.

15.
16.
17.
18.

19.
20.

. . / . .
, . . , . . // . 2004. 10. . 12641267.
. . - /
. . , . . // . . -. . . 1997. 4. . 137145.
. . : . . . / .
. , . . , . . .: , 2001. 304 .
. . / . . , . .
// . 1998. 10. . 8393.
.. / .. //
- 2006. . 18.
. : , 2006. . 2426.
Tekle K. Vegetation on hill slopes in southern Wello, Ethiopia: Degradation and regeneration / K. Tekle,
I. Backeus, J. Skoglund, Z. Woldu // Nord. J. Bot. 1997. V. 17. 5. . 483493.
.. (Betula pendula
Roth.) / .. //

. 4. .: - , 2000. . 71-72.
.. / .. ,
.. , .. .: . . 1998. 287 .
.. : . / [..
, .. , .. , .. , .. , .. , ..
, .. , .. , ..]. ., 2000. 318 .
Kozlov M.V. Fluctuation asymmetry of birch leaves increases under pollution impact / M.V. Kozlov, B.J.
Wilsey, J. Koricheva, E. Haukioja // J. Appl. Ecology. 1996. 33. . 14891495.
.. / ..
, .. // . .
. 11. 2007. . 2836.
.. ( ) /
.. // . 2001. 3. . 177191.

(
) / ; . 16.10.03. 460-. ., 2003. 24 .
.. (Betula platyphylla Sukacz.)
,
( ): . . . . : 03.00.16 / ..
, 2006. 18 .
.. / .., .. //
. 4. : , 2009. . 137139.
..
- / .. , .. , .. //
. 2004. . 65, 5. . 433441.
. . / .. . .: , 1970. 367 .
..
(Tilia cordata Mill.) / .. //
- : . .
1- ( 20 -20 , 2010). : ,
2010. . 4346.
.. :
/ .. , .. // . -. : . .
. .-. ., , ., 1996. . 2. , 1996. . 1820.
.. / .. // . .
. 1995. 4. . 2728, 99.

134

MORUS ALBA L.
21. Tsvetkova N. Effect of air pollution on carbohydrate and nutrients concentration in some deciduous tree
species / N. Tsvetkova, D. Kolarov // Bulg. J. Plant Physiol. 1996. Vol. 22. 12. . 5363.
22. ..
/ .. // 2006. . 18. .
: , 2006. . 4548.
23. Lorenc-Plucicka G. Pobieranie cukrw przez licie klonu srebrzystego (Acer saccharinum L.) idziaanie
siarczynu / G. Lorenc-Plucicka // Arbor. kor. 1993. Vol. 38. P. 5364.
24. Lorenc-Plucicka G. Wplyw siarczynuna fotosyntez i transport sacharozy w liciach topoli / G. LorencPlucicka, J. Figaj // Arbor. kor. 1993. Vol. 38. P. 7578.
25. ..
/ .. , .. , .. // .
. . . . . . .
. . [, 1996]: . . . 1. ., 1996. . 6162.
26. .. .
/ .. // . . (ECOLOGY-2006).
. . .-. . . , 4-7 . 2006 . .: -,
2006. . 95.
27. .. . / .. ,
.. // : . . . .-. ., , 1620 , 1997. . 1. , 1997. . 6667.
.. Morus alba L.
/ .. , .. , .. //
. .. . , . 2011. . 24 (63), 2.
. 129-135.
Morus alba L.
. ,
Morus alba L.
.
: Morus alba L., , ,
, .
Ibragimova E.E. Fluctuating asymmetry leaves Morus alba L. as bioindicators of aerotechnogenic
pollution urboecosystems / E.E. Ibragimova, I.V. Bandak, A.S. Drozd // Scientific Notes of Taurida
V.I. Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 129-135.
The article deals with the estimate of stability changes in the development of Morus alba L. in biotopes with
different level of technogenic work. It was determined that the deterioration of the urbanized environment
ecological not condition causes the decrease of stability in development of Morus alba L. and can testify their
tolerance to aerotechnogenic pollution.
Keywords: Morus alba L., technogenic pollution, development sability, fluctuating asymmetry, tolerance.

17.05.2011 .

135

. . .
, . 24 (63). 2011. 2. . 136-142.

636.591+612.664:616.89-008.441+591.36

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E-mail: nina_karpenko@mail.ru

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, ,
.

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.
.

1.

Interaction with stressed mothers affects corticosterone levels in pups after reunion and impairs the
response to dexamethasone in adult mice / A. Moles [et al.] // Psychoneuroendocrinology. 2008. Vol.
33, 4. P. 462470.
2. Cerebrospinal fluid concentrations of biogenic amines and corticotropin-releasing factor in adolescent
non-human primates as a function of the timing of adverse early rearing / S.J. Mathew, J.D. Coplan, E.L.
Smith [et al.] // Stress. - 2002; Vol. 5. . 185193.
3. Long-term effects of early-life environmental manipulations in rodents and primates: Potential animal
models in depression research / C.R. Pryce, Ruedi- D. Bettschen, A.C. Dettling [et al.] // Neurosci
Biobehav Rev. 2005. - Vol. 29. . 649674.
4. Xenobiotic phenols in early pregnancy amniotic fluid / S.M. Engel, B. Levy, Z. Liu [et al.] // Reprod.
Toxicol. 2006. Vol. 21. P. 110112.
5. Patisaul, H.B. Neonatal exposure to endocrine active compounds or an ERbeta agonist increases adult
anxiety and aggression in gonadal intact male rats / H.B. Patisaul, H.L. Bateman // Horm. Behav. 2008.
Vol. 53. 4. P. 580-588.
6. Effects of Genistein or Soy Milk During Late Gestation and Lactation on Adult Uterine Organization in
the Rat [Text] / C. L. Hughes, G. Liu, S. Beall [et al.] // Experim. Biol. Med. 2004. 229. P. 108117.
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Pathol. 2000. Vol. 28, 3. P. 420431.
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pups? [Text] / A. Moles, R. Rizzi, F. R. D'Amato // Dev. Psychobiol. 2004. Vol. 44, 4. . 230
237.
9. 90-Day Feeding and One-Generation Reproductive Stady in Crl:CD BR Rats with 17beta-Estradiol
[Text] / L. B. Biegel, A. N. Hirshfield, J. C. OConnor [et al.] // Toxicol. Sciences. 1998. Vol. 44. P.
116142.
10. Genistein Soy Complex /
.. , .. , .. , .. //
: . .-. . , 3-4 . 2011 .
. - 2011. . 29-30.

141

.., ..
11. Effects of Dietary 17beta-Estradiol Exposure on Serum Hormone Concentrations and Testicular
Parameters in Male Crl:CD BR Rats / J. C. Cook, L. Johnson, J. C. OConnor [et al.] // Toxicol.
Sciences. 1998. Vol. 44. P. 155168.
12. [Te] / ..
, .. , .. [ .] // . . . 2010. 1 . 8289.
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Behav. 1996. V. 54, 1. P. 312.
14. , . . : . . . / . . . . : . .,
1990. 352 .
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social stressors / R.J. Rodgers, J.C. Cole // Physiol. Behav. 1993. Vol. 53. P. 83-388.
16. , ..
- / .. , .. ,
.. // . - 2010. - 4. . 78-84.
17. , .. S100 -:
- / .. , .. , .. // .
. . 2003.- . . 24-26.
18. , .. : ,
/ .. // . - 2006.
. 4, 6. - . 27-30.
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chronic estrogen and progesterone / E. Redei, L. Li, I.Halasz [et al.] // Neuroendocrinol. 1994. - Vol.
60, 2. - . 113-23.
20. Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and
corticosterone in middle-aged male rats / V.Z. Ajdzanovi, B.T. Sosi-Jurjevi, B.R. Filipvi, [et al.] // Biol
Res. 2009. - Vol 42, 1. . 13-23.
21. , ..
/ .. , .. // . .
. 2008. 6. . 20.
.. ,
/ .. ,
.. // . .. .
, . 2011. . 24 (63), 2. . 136-142

, c
/ .
- .
.
.
: , , , , , .
Karpenko N.O. The level of anxiety of adult rats were stressed and feed substances with estrogenic
property at early postnatal period / N.O. Karpenko, N.P. Smolenko // Scientific Notes of Taurida
V.Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 136-142.
The level of anxiety of adult rats which were exposed to maternal separation stress or abundance of substances
with estrogenic property during suckling has been examined. The imprinting anxiety-depressing events had
been found out in adult males from the Stress group. The weakening of this anxiogenic effect of stress is
observed at males were exposed to both stress and substances with estrogenic property. The manipulations
during suckling have not influence on the behaviour of adult females.
Keywords: stress, phytoestrogens, estradiol, suckling, anxiety, rats.

12.05.2011 .

142

. . .
, . 24 (63). 2011. 2. . 143-149.

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0,82.
QSAR-,

---D, ClogP.

1.
2.
3.

Bioluminescent bacteria as indicators of chemical contamination of coastal waters / M. E. Frischer, J .M.


Danforth, T. F. Foy [et al.] // J. Environ. Qual. 2005. Vol. 34, 4. . 13281336.
. . , ,
/ . . // . 2008. 1(5). . 5053.
Toxicity assessment of atrazine and related triazine compounds in the microtox assay, and computational
modeling for their structureactivity relationship / P.B. Tchounwou, B. Wilson, A. Ishaque [et al.] //
Intern. J. Mol. Sci. 2000. Vol. 1. . 6374.

148

-
4.
5.
6.

---D-
/ .. , .. , .. , ..
// . . . 2009. . 7, . 4(28). . 3040.
Farr M. Assessment of the acute toxicity of triclosan and methyl triclosan in wastewater based on the
bioluminescence inhibition of Vibrio fischeri / M. Farr, D. Asperger, L. Kantiani // Anal. Bioanal.
Chem. 2008. Vol. 390, 8. P. 19992007.
Gellert G. Sensitivity and Significance of Luminescent Bacteria in Chronic Toxicity Testing Based on
Growth and Bioluminescence / Georg Gellert // Ecotoxicology and Environmental Safety 2000.
Vol. 45, 1. P. 8791.

.. - (SAR) --D- / .. , .. ,
.. , .. //
. .. . , . 2011. . 24 (63), 2. . 143-149.
, ,
-O--D-. , '
, . QSAR CLOGP.
: : , , , , QSAR.
Katsev A.M. Structure-activity relationship for aryl-O--D-glucosaminides studied by luminescent
bacteria biotesting / A.M. Katsev, V.O. Kuruanov, T.A. Chupakhina, V.Ya. Chirva // Scientific Notes of
Taurida V.I. Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2.
. 143-149.
The biological action of new derivatives of aryl-O--D-glucosaminides is studied by luminescent bacteria
biotesting. It is shown that inhibition effect of bacterial luminescence is related to the certain functional
groups, and depends on lipophilicity of substances. The QSAR-models of dependence between biological
effect and ClogP values are present.
Keywords: bioluminescence, luminescent bacteria, biotesting, QSAR.

19.05.2011 .

149

. . .
, . 24 (63). 2011. 2. . 150-156.

612.82 612.13



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1. .. / .. .- .: , 1973.- .33-43.
2. .. / .. // .- 1998.-2.
.135

3. .. / .. .- Cosmetic Karl Hadek International,


1998. . 342.

4. .. / .. // ,
.- , 1999.- .787-792.

5. .. / .. .- ., 1984. 342 .
6. : . . . . / [
.., .., .. .].- .: , 1991.- 512 .

7. : /.-. .. .- /: , 2002.- 704 .


.. /
.. , .. , .. //
. .. . , . 2011. . 24 (63), 2. . 150-156.
, , .
.
: , , , , , , .
Kirillova A.V. Comparative estimation of influence of essential oils on status
of man/ A.V. Kirillova, S.A. Panova, L.D. Lesova, // Scientific Notes of Taurida V.I. Vernadsky National
University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 150-156.
The actions of essential oils of lavender, clary, pine-tree and lemon are investigational. The changes of indexes
of basic rhythms are shown EEG and the state of man at the action of essential oils.
Keywords: essential oils, lavender, clary, lemon, pine-tree, EEG, tests.

17.05.2011 .

156

. . .
, . 24 (63). 2011. 2. . 157-160.

617-089.843:611-013


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.. : .
. . . / ..-,-2008.-19.
// : . .
. / .. , .. , .. [ .] - , 1991. - . 176-177.
.. .- 2 / .., ..,
.. - -: 2001.- 112 .
Gabler C. Studies on the physiological function of thiamine / C. Gabler // J. Biol. Chem, 1961.- V.12.
. 3112-3120.
Kiessling K.H. Thiamine diphosphate in grouring tissues/ Kiessling K.H., Lundquist I.I, Exp. Cell. Res. 1961.-V.26, 1.-p.189-197
.. /. ..- : , 1972.- 318.

[ ], . .- . ., .- 2009.-. II.- . 247.
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..


/ .. , .. , .. , .. //
. .. . , . 2011.
. 24 (63), 2. . 157-160

-,
,
.
: , -, ,
.
Kobylnik S.N. Activity multienzyme piruvatdegidrogenase and -ketoglutaratedehydrogenase
complexes under the condition of transplantation of embryonic muscle / S.N. Kobylnik, E.V. Kulibaba,
A.A. Yanchukova, S.A. Petrov // Scientific Notes of Taurida V.Vernadsky National University. Series:
Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 157-160.
Allotransplantation leads to increase activity of -ketoglutaratedehydrogenase, pyruvat dehydrogenase and the
oxidation of endogenous substrates in the ventral and in the skeletal muscle.
Keywords: allotransplantation, -ketoglutaratedehydrogenase, pyruvatedehydrogenase, the endogenous
substrates.

23.04.2011 .

160

. . .
, . 24 (63). 2011. 2. . 161-168.

159.938 : 612.172.2



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1.
2.
3.
4.
5.
6.
7.
8.

Rosenman R.H. Modifying type A behavior pattern / R.H. Rosenman, M. Friedman // J. Psychosom. Res.
1977. v. 21. 4. P. 323-331.
Harbin T.J. The relationship between the type A behavior pattern and physiological responsivity: A
quantitative review / T.J. Harbin // Psychophysiology. 1989. v. 26. 1. P. 110-119.
Pfiffner D., Psychophysiological reactivity in type A and B woman during a rapid information processing
task / D. Pfiffner, P. Elsinger, R. Nil, R. Buzzi, K. Bttig // Experientia. 1986. v. 42. 2. P. 126131.
Perkins K.A. Heart rate change in type A and type B males as a function of response const and task
difficulty / K.A. Perkins // Psychophysiology. 1984. v. 21. 1. P. 14-21 (1984).
Contrada R.J. Psychophysiological correlates of type A behavior: Comments on Houston (1983) and
Holmes (1983) / R.J. Contrada, R.A. Wright, D.C. Glass // J. Res. Pers. 1985. v. 19. 1. P. 1230.
Lanfranconi B. Type A behavior and psychophysiological reactivity: A 27-month follow-up / B.
Lanfranconi, D. Pfiffner, R. Nil, K. Bttig // Psychophysiology88: Proc. 4th Conf. Int. Organ.
Psychophysiol. (Prague, Sept. 12-17), Prague (1988). P. 157.
.. : / .. ,
.. , .. , .. , .. , .. //
. 2002. . 28, 1. . 130-143.
..
/ .. // . 2003. 32. . 14-23.

.. i iii i i i
/ .. // . .. .
, . 2011. . 24 (63), 2. . 161-168.
i i iii ii i i
i i, i i i.
i iii, i.
i i i iii ii ii i
. , i i i ( )
(i).
: , i, iii, ii
, .
Konareva I.N. Peculiarities of cardiointervalographic induces in persons with behavioral types of A and
B / I.N. Konareva // Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology,
chemistry. 2011. Vol. 24 (63), No 2. . 161-168.
We measured individual induces of the heart rate variability in representatives of different types of personality
classified according to the Jenkins questionnaire. A coronary-prone type A is characterized in the terms of
cardiointervalography based on the statistical criteria. Statistical and spectral specificities of
cardiointervalograms typical of individuals with the personality types A and B have been demonstrated. As
was found, distributions of the values of the stress index (by Bayevskii) within the above types are
heterogeneous (bimodal).
Keywords: coronary-prone type A, Jenkins questionnaire, cardiointervalography, heart rate variability, power
spectra.

24.04.2011 .

168

. . .
, . 24 (63). 2011. 2. . 169-178.

594:612.884/615.849.11

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4,00
3,76
0,0530,007
0,0410,004
3,8130,061
2,5220,826
4,92
--0,0590,009
--4,9310,087
--6,74
6,4
0,0590,010
0,0490,006
3,9780,097
2,3060,201
--9,14
--0,0600,006
--2,5230,234

174

HELIX ALBESCENS



. , 0,030 ..
0,047 ..
9,14
0,0470,005 ..
,
, ,
2,51; 2,98; 4,00; 4,92 6,74 .
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, 4,92 6,74
0,059 .. (. 2).

.
0,08

0,07

(..)

0,06
0,05
0,04
0,03
0,02
0,01
0
2,42-2,51

2,67-2,98

3,37

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()

. 2.

.
,

. ,
3,37d; 9,14d, ,
4,92 . ,
. ,
, ,
, ,
. ,
.

175

..


.

. .. (1968, 1974)

,
90, [31, 32].
R. Wever (1973)
, [33].

, .

. , ..
[34]. , 60 0-2,5
[35].
,
, ,
,
,
.
,
,
, .. .

, ,
,
:
(- , =-6,422,54%)
(14 , =5,901,74%)
,
.

1.
2.
3.
4.

..
/ .. // .
1999. . XLIII, 5. . 311.
.. (
.. ) / .. , .. . .: - ,
2000. 374 .
Adey W.R. Frequency and power window in tissue interactions with weak electromagnetic fields / W.R.
Adey // Proc. IEEE. 1980. Vol. 68 (1). P. 119.
Kavaliers M. Repeated naloxone treatments and exposures to weak 60-Hz magnetic fields have
'analgesic' effects in snails / M. Kavaliers, K. Ossenkopp // Brain Res. 1993. Vol. 620(1). P. 159
162.

176

HELIX ALBESCENS
5.
6.
7.
8.
9.
10.
11.
12.

13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.

Jenrow K.A. Weak extremely-low-frequency magnetic field-induced regeneration anomalies in the


planarian Dugesia tigrina / K.A. Jenrow, C.H. Smith, A.R. Liboff // Bioelectromagnetics. 1996. Vol.
17 (6) P. 467474.
Prato F.S. Extremely low frequency magnetic fields can either increase or decrease analgaesia in the land
snail depending on field and light conditions / F.S. Prato, M. Kavaliers, A.W. Thomas //
Bioelectromagnetics. 2000. Vol. 21. P. 287301.
Exposure to a hypogeomagnetic field or to oscillating magnetic fields similarly reduce stress-induced
analgesia in C57 male mice / C. Del Seppia, P. Luschi, S. Ghione [et al.] // Life Sci. 2000. Vol. 66
(14). P. 12991306.
Shielding, but not zeroing of the ambient magnetic field reduces stress-induced analgesia in mice / E.
Choleris, Seppia Del, A.W. Thomas [et al.] // Proceedings. Biological sciences. The Royal Society.
2002. Vol. 269. P. 193201.
..
/ .. , .. // , : , 2006. 508 .
Sherrington C.S. The integrative action of nervous system / Sherrington C.S. Yale University Press:
New Haven, 1906. 411 p.
Besson J.M. Peripheral and spinal mechanisms of nociception / J.M. Besson, P. Chaouch // Physiological
Reviews. 1986. Vol. 67. P. 88186.
.. Helix albescens
/ .. , .. //
. .. . .
2009. . 22 (61). 3. . 7582.
..
/ .. , .. , .. // . 2009. . 17(2).
C. 174178.
.. / .. : ,
1976. 127 .
.., .., .. -
Exel / .., .., .. .: ,
2000. 319 .
. Statistica. : . 2- . /
. .: , 2003. 688 .
..
/ .. , .. //
. 1981. . 15, 4. C. 5758.
.. ,
/ .. //
. 1973. . 53. . 5152.
..
/ .. , .. //
. , 1973. . 2123.
.. ( ) / ..
, .. . . -, 1984. 190 .
.. / ..
// . 1955. . 103, 3. . 513515.
Brown F.A. Inter organisms and environmental influence through extremely weak electromagnetic field /
F.A. Brown, C.S. Chow // Biology Bull. 1973. Vol. 144. P. 437439.

/ .. , .. , .. [ .] // . 1992. . 37, 4.
. 738744.
..
, / .. , ..
, .. // . 1992. . 37, 4. . 745749.

177

..
25. ..
/ .. // III
. , 1975. .194195.
26. ..
Drosophila m. / .. , .. //
. , 1973. 82 .
27. ..
/ .. , .. , .. //
. , 1973. . 83.
28. Becker G. Korrelation zwischen der Frabaktivitt vonn Termiten und der geomagnetischen Aktivitt / G.
Becker, W. Gerisch // Zeitschrift fr angewandte Entomologic. 1977. Vol. 84, I. 4. P. 353388.
29. Becker G. Influence of Magnetic, Electric and Gravity Fields on Termite Activity / G. Becker // Material
and Organisms. 1976. Vol. 3. P. 407411.
30. Becker G. Communication between Termites by Biofields / G. Becker // Biol. Cybernetics. 1977. V.
26. P. 4145.
31. ..
/ .. // .
1968. . 187, 6. . 14291431.
32. .. / .. .: , 1974. 176 .
33. Wever R.A. Human circadian rhythms ubder the influence of weak electric fields and the different
aspects of these studies / R.A. Wever // Int. J. Biometeorol. 1973. Vol. 17, No. 3. P. 227232.
34. ..
: . . . . . : .
03.00.13. / .. . , 2007. 20 .
35. Extremely low frequency magnetic field exposure modulates the diurnal rhythm of the pain threshold in
mice / Y.M. Choi, J.H. Jeong, S.K. Jeong [et al.] // Bioelectromagnetics. 2003. Vol. 24 P. 206210.
.. Helix albescens /
.. // . .. . ,
. 2011. . 24 (63), 2. . 169-178.
, ,
, Helix albescens. ,
: I - 6,42%
(), II - (
5,90 %, III . ,

.
: , , , .
Kostyuk A.S. Nociception of snails Helix albescens in shielding in copper shield / A.S. Kostyuk //
Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry. 2011.
Vol. 24 (63), No 2. . 169-178.
The influence of an electromagnetic shield, made of the textolite, which weakens the electric field and radio
frequency alternating magnetic field, the parameters of nociception mollusc Helix albescens. It was found that
changes in nociception are phase character: I stage an increase of nociceptive sensitivity to 6.42%
(hyperalgesia), II phase - the development of antinociceptive effect (decrease in nociceptive sensitivity to
5,90%, III phase stabilization of parameters of nociception at baseline data . It is shown that the presence of
snails in the copper shield is a tendency to change the parameters infradian rhythmicity of nociception.
Keywords: nociception, electromagnetic shielding, infradian rhythmicity, snails.

14.06.2011 .

178

. . .
, . 24 (63). 2011. 2. . 179-185.

582.475:630*114


PINUS SYLVESTRIS
..
, . ,
, . , . ,
E-mail: krest.igor@mail.ru

Pinus sylvestris . ,
, .
: , Pinus sylvestris, , , ,
, .

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179

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, [2].
,
,
.

(Pinus
sylvestris L.) 2010 .
6,3 , 1300
-.
40 .

-2. ( ) 0,5
, 3 .

,
100, 250, 500 3.
(
) , ,
.

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:

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, .

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, .
: ,
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. -
20 40 60 80 . 20 30 6
10 %, ,
,
[4].
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. , , ,
, .

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, .
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.
, 0,90
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1,25; 1,25.
,
[5, 6].
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1,0 1,2; ( , ) 0,2

181

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.

.
55 65 % , 50 55,
50 %.
,
. ,
15 25 % ( )
, 10 15 % - , 10 % - .
,
[7].
,
, ,
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( , ). 1,
, 0,77 0,85,
1,0-1,2 /3.
. 20 30
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.
50% , 0,99 3.
, ,
50%, .
,
, 90%.
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,
(, .).

.

() , 5565 %
. 50 %,
.
1,
( 62 71 %) . 0 20
[4], (
20 30 ). ,
. 20
30 , .
.
, , 0 10

182

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.
0,5
, 0,9.
, 0,5,
,
, () .
(. 1),

0,5, , .
1
( )

1.

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(90%
)
2.

0,5
(50%
)

3.

- . -
,

/
%
%
%

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35,87

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0,82

69,11

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534,02

0,99

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183

..

60


0,9
(90% )

50
40


0,5
(50% )

30
20

10
0
0-10

10-20

20-30

.1.

,
, 2 2
.
, ,
15 %
30-40 % . ,

( ), 30 40%.
, ,
.

1.
.
, ,
, , 0 10, 10
20 . ,
. 20 30
90 %
1,02 3,
. 50% ,
0,99 3. ,
, 50%,
. ,
,
90%.

184

2. (
62 71 %) . 0 20
, ( 20
30 ). ,
. 20 30
, , , .
,
.
3. 0,5 ,
0,9.
, 0,5,
,
, () .

1.
2.
3.
4.
5.
6.
7.

.., ., .. . .: , 1991 264 .


.. . .: , 2004 243 .
.., .., .. . .: ,
1987. . 18-27.
. . , .
.: , 1967 365 .
.., , .., .. . .:
, 2000 387 .
.., .. .
.: , 2009 82 .
.., .. . : . ,
2005. 74 .

.. Pinus sylvestris
/ .. // . .. .
, . 2011. . 24 (63), 2. . 179-185.

Pinus sylvestris . , '
, .
: , Pinus sylvestris, , , ,
, .
Krestyanishin I.A. Study of the soil structure in forest cultures of Pinus sylvestris yayla /
I.A. Krestyanishin // Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology,
chemistry. 2011. Vol. 24 (63), No 2. . 179-185.
In this paper, bringing the results of investigation of the structure of forest soil in cultural tours of Pinus
sylvestris jajle. The characteristic differences in their structure, due to the fullness of forest crops, and also
given a comparison with open areas jajly.
Keywords: soil structure, Pinus sylvestris, forest plantations, Yayla, aeration porosity, soil moisture, density,
composition of the soil..

12.05.2011 .

185

. . .
, . 24 (63). 2011. 2. . 186-194.

633.812.754: 578.083

IN VITRO LAVANDULA ANGUSTIFOLIA MILL.


..1, ..2
1

, ,
- , ,
E-mail: latushkina2004@mail.ru
2

in vitro
(INSV).
: , , in vitro.

,
,
[1].


- (, , ),
(), .
, ,
, 26-50%, ,
[2].
,
.
,
.


. ,
,
: ,
[3].

( 3642 0).
, [4]:
1) ,
(
);

186

IN VITRO LAVANDULA ANGUSTIFOLIA MILL.

2) ,
;
3) ;
4)
, ,
. , J.H. Wu I. Rappaport
, ,
[. 5].
in vitro ,
[6].
, 80 % [6], 100 % [7]
.
in vitro
.


L. angustifolia Mill. .

(, ELISA) (indirect ELISA)
(DAS-ELISA) [8] 96-
(Labsystems, ). 1
3 () (0,1 /)
1:5 (: ). 5000 /.
15 ., .
1:1 (: ).
,
(TMV, Tobamovirus), (AMV, Alfamovirus),
(CMV, Cucumovirus), - (PVX, Potexvirus),
(TRV, Tobravirus), (BYDV,
Luteovirus), (WSMV, Potyvirus),
(INSV, Tospovirus).
, (Sigma,
). (N-n-)
1,0 /, 3 NaOH.
ELISA- Dynex Technologies 405 .
,
2 .
3- .
in vitro [3] (
) ,
25-26 5 18 30 .
15-20 .

187

.., ..

, 25 ,
2 371 . :
371 , 23 /2, 16 ,
6070 %.
-10. ,
2526 .
,
, . 2- 20-
. ,
20 .


in vitro.
371 0 .
,
35 .

,
[9], 38
5,3 % , , ,
14,3 %, 20 [10].
in vitro
,
,
.

.
. ,
INSV, (405=0,613 . .)
, (405=0,445 . .).
, TMV, AMV, TRV, WSMV,
BYDV, CMV PVX, .
INSV Impatiens necrotic spot virus, ,
Tospovirus, Bunyaviridae [11].
1990 Law M.D. Moyer J.W. [12].
, , , , , , [12].
60 [12], Lamiaceae Lamium,
Lavandula, Melissa, Mentha, Salvia.
,
,
(. 1). 6-8-
, 14,3-33,3 % .
,

188

IN VITRO LAVANDULA ANGUSTIFOLIA MILL.

100 % 10- .
57,1-33,3 % 12-
, 16-20-
42,9-6,7 %,
.

, %
100
90
80
70
60
50
40
30
20
10
0

10

12

14

16

18

20

, %
100
90
80
70
60
50
40
30
20
10
0

10

12

14

16

18

20

. 1 () ()
in vitro
, in
vitro 10 , 57,1-

189

.., ..

100,0 %. ,
(. 1).
1
in vitro

, %

100,00
66,67
100,00
100,00
100,00
57,14
100,00
66,67

10

,
,
.
11,50 1,50
3,00 0,00
12,08 1,44
1,00 0,00
10,75 2,84
2,00 0,00
11,43 1,85
1,00 0,00
22,50 1,50
2,25 0,48
25,25 3,09
1,50 0,19
20,00 2,18
3,50 0,50
19,67 2,10 1,00 0,00**

: **=0,01.

,
. 10
100,0 %
, 66,7 %.
66,7%,
57,1%.
, .
, ,
, , .
1,7-2,1 .
,
- . , 371
,
, , ,
(),
2-4
. ,
in vitro ,
33,3 % , 9,6 %
,
.


, 3-5 , 2-

190

IN VITRO LAVANDULA ANGUSTIFOLIA MILL.

7 .
5
(. 2).
2
in vitro
(50 )

, %

100,0

100,0

100,0

100,0

21,42 1,41

23,25 3,01

44,20 2,89

47,00 5,43


, .

4,74 0,44

5,50 0,48

6,90 0,54

7,50 0,29

100,0

100,0

70,0

60,0

3,42 0,34

6,750,48***

1,36 0,13

3,67 0,67**

1 : 8,16

1 : 12,25

1 : 7,85

1 : 9,70

,
%

, .

:
=0,01, ***= 0,001.

**

( )

100 %.
, ,
. ,
,
(. 2).
, , 6-7
3-4 .
10 %,
3-4 , 1-2
. -

.

191

.., ..

18 ,

[13].
in vivo
.

. 2 in vitro
INSV -
. ,
INSV
.
INSV
70 %,
0,7 60 % .
INSV -. , ,
,
, ;
,
in vitro ,
, .

(10 %),
100 % ,
(. 3).

192

IN VITRO LAVANDULA ANGUSTIFOLIA MILL.

, ,
,
, . ,
. 3, ,
in
vitro 371 .
3


0,7
in vitro

0,7
in vitro


N=10
,
,
.
,%
.

1 : 13,28

132,8

60,0

79,7

1 : 12,25
122,5

70,0

85,8

1 : 9,38

93,8

100,0

93,8

1 : 9,70

97,0

100,0

97,0

. N=10 .

, INSV
in vitro,
,
in vivo:
1 2-2,5 ;
;
, , ,
, ;

in vitro; 1-2 4-5
.

1. , in vitro
371, 10 , 16 ,
,
100,0 %.
2.
70,0 % 100,0 % .

193

.., ..


1.
2.
3.
4.
5.
6.

7.
8.
9.
10.
11.
12.
13.

/ .., .., .. . //

. : , 2004. . 64-79.
.. / .. ,
.. , .. // . . . XXIII. . 1992. .
48-54.
: . ./ ., ., .
. .: , 1986. 480 .
.. / .. . .:
, 1979. 246 .
.. / .. //
. 1992. . 112, . 4. . 528540.
..
/ .. ,
.. , .. //
. . . . . 1997. . 119. . 7-34.
.. in vitro / .. , ..
// . . . 2002. . 86. . 59-61.
. . 2. / . . . ., 1991. . 152-165.
Chauhan Y.S. Thermostabilities of Cell Membrane and Photosynthesis in Cabbage Cultivars Differing in
Heat Tolerance / Y.S.Chauhan, T. Senboku // J. Plant Physiol. 1996. V. 149. P. 727-734.
/ .. , .. ,
.. . / . .. . .: , 2000. 640 .
Virus taxonomy. Sixth reports of the International Committee on Taxonomy of Viruses / Eds. by F.A.
Murphy et al. Springer-Verlag, Wien, 1995. 587 p.
Ruter J.M. Gitaitis Impatiens Necrotic Spot Virus On Woody Ornamentalsin Georgia / J.M. Ruter, D.
Ron // SNA Research Conference. 1993. Vol. 38. P. 212.
.. (Lavandula angustifolia
Mill.) / .. , .. // . 2009.
. 99. . 115-118.

.. in vitro Lavandula angustifolia Mill. / .. ,


.. // . .. .
, . 2011. . 24 (63), 2. .186-194
in vitro
(INSV).
: , , in vitro.
Manushkina T.N. Thermotherapy in vitro of plants Lavandula angustifolia Mill./ T.N. Manushkina,
Bugaenko L.A. // Scientific Notes of Taurida V.Vernadsky National University. Series: Biology, chemistry.
2011. Vol. 24 (63), No. 2. . 186-194.
The comparative study of efficiency of technique of improvement thermotherapy in vitro for clearing plants of
lavender of the Impatiens necrotic spot virus (INSV) is conducted.
Keywords: lavender, viruses, thermotherapy in vitro.

11.05.2011 .

194

. . .
, . 24 (63). 2011. 2. . 195-202.

:616.2-796-05.072.2

..
. .. , ,
E-mail: tnu-fr@rambler.ru
,

.
.
: , , , .


.


. ,
, ,


[1].
,
, ,
, , [2].
, , ,
. , ,


,
[3].
,

.
, .

195

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10
, 35 .
,
.

.
,
.
[4].
,
, , .

,
, , .

: -2, ,
(). PWC 150 [5].

[6].


,
.
. 1.

.
1.
(=35)
(=35) , (Sx)

.
.
2,
.
.,
/2
,

137,31,2
(25)

29,80,8
(25)

62,91,1
(25)

143,02,3
(75)
<0,05

36,091,9
(75)
<0,01

69,42,0
(90)
<0,05

196

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4,90,6
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,
.

,
/

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(50)

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,

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.
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.

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,

.
.
,

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50 .
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.

.
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. 25
.
50 ,
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,
.
,
,
, (. 2.).

197

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(=35) 6 (Sx)

.
.
,

.
.,

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/2

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.

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/

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(25)

310,6
(25)

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(50)

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144,02,8
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361,9
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,

.
.

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. ,
. .
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1
6 .

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.

198

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,
.
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.
.

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, 60,0 %
, 40,0 %
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.
. ,
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.

.
.
,
,
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, 45,0 %

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%

199

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,
. ,
, 5
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.

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. .. . , . 2011. . 24 (63), 2. . 195-202.
, '

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: , , , .
Markina I.V. Physical development and biopower descriptions of organism of girls with diffuse
muscular gipotoni / I.V. Markina // Scientific Notes of Taurida V.I. Vernadsky National University.
Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 195-202.
It is rotined on the basis of the conducted researches, that girls with a diffuse hypomyotonia behave to
mikrosomatical and mikromezosomatical somatotips and characterized the slow rates of physical
development. The offered program of physical exercises is instrumental in substantial growth of functional
backlogs of optimization of power exchange.
Keywords: girls, somatotipirovanie, power potential, physical exercises.

09.06.2011 .

202

. . .
, . 24 (63). 2011. 2. . 203-213.

:612.172.1:612.766.1:612.81]-057.875:796/799




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212


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7.
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9.
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13.
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.. , .. // .. 1988. 4. .29-31.
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/ .. //
. .. . , . 2011. . 24 (63),
2. . 203-213.


, .

, .
: , , ,
"".
Minin E.N. The autonomic regulation of cardiac rhythm in female students with insufficient physical
activity at different incentive effects / E.N. Minin // Scientific Notes of Taurida V.I. Vernadsky National
University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 203-213.
In the group with reduced functional reserves and low levels of physical health correction mechanisms of the
vegetative regulation in the face of their optimization as is possible under the influence of a combination of
physical exercise and essential oils, as well as with the independent use only essential oils. In the last more
than adequate regulation of the adaptation processes is not due to the accumulation of functional reserves, and
as a result of the adaptation voltage.
Keywords: physical activity, regulation of the autonomic nervous system, "Poliol".

13.05.2011 .

213

. . .
, . 24 (63). 2011. 2. . 214-223.

338.27



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E-mail: heliomechanics@gmail.com
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http://meteocenter.net/meteolib/sun2.htm
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http://www.bg-znanie.ru/article.php?nid=33998
3. .., . / .. //
, 1966. 168, 2 . 284-287
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[ .] // , 1996, ., 93, . 53 57.
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, .1973, . 32 42.
6. . ., , .,1981
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, 1995 .3-206.
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222


12. . /
[ .., .., .., .. . ] . - :
"", 2003. - 448 .
13. ..
. / .. ,
.. // ...
, . 2007. .20 (59), 1. . 168-174.
14. Standish E.M. An approximation to the outer planet ephemeris errors in JPL's DE200 // Astron.
Astrophys. vol. 233, pp. 272-274. 1990.
15. .. .
.
... , . 2007. .20(59), 1. .175-188.
16. .. 11- . //
... , .
2007. .20 (59), 4. .67-73.
17. .. - . /
.. //
... - . 2010. .23(62), 3.

.. /
.. // . .. .
, . 2011. . 24 (63), 2. . 214-223.
.
, ,
,
. , () '
DE200 ,
, , - . ,
' .
, ii, i.
: , , , .
Narmanskiy V.Y. Dynamics of vectors of planetary speeds and processes in biosphere / V.Y.
Narmanskiy // Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry.
2011. Vol. 24 (63), No 2. . 214-223.
Article is devoted to the theory and practical application of new cosmophysical index. As against an astrology
and mountain heomechanics where dynamics are considered in geocentric to system of coordinates, a basis of an
index became vectors of speed of the planets, considered in heliocentric system of coordinates. The index, called
"heliomechanics" (HM) calculated a computer program was written based on the DE200 ephemeris, and is used
as a tool for research and prediction of biological, medical, social, economic and other time series. It is shown
that biological processes have a significant correlation with the dynamics of the velocity vectors of the planets. It
is suggested that the speed of the planets is synchronizing and synchronized rhythms of the biosphere.
Keywords: velocity vector, Mercury, Mars, and cycles.

13.06.2011 .

223

. . .
, . 24 (63). 2011. 2. . 224-230.

612.72:615.825



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. .. . , . 2011. . 24 (63), 2. . 224-230.
8-10 ,
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Popolitov R.A. Trial of rideterapy as a means of functional brain activity of children suffering from CP
/ R.A. Popolitov, V.N. Konovalchuk, E.V. Arkhangelskaya, J.S. Chenchik // Scientific Notes of Taurida
V.I. Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 224-230.
In this study the effect rideterapy on bioelectric activity of the brain of children 8-10 years old suffering from
cerebral palsy. Shown that after raytterapiyi course, in children who have it, make significant positive changes
in EEG parameters in comparative analysis with data of control group children.These changes are expressed in
the improvement of fine motor skills of upper extremities, which was proved by Pearson correlation analysis.
Keywords: cerebral palsy, spastic diplegia, rideterapy, electroencephalogram, index rate,. hemispheric
asymmetry, fine motor skills.

11.06.2011 .

230

. . .
, . 24 (63). 2011. 2. . 231-239.

574.472:595.72(477.75)

-
(INSECTA, ORTHOPTERA)
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E-mail: vpbiscrim@mail.ru
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1
Orthoptera Oliver,1789 -

Phaneroptera nana Fieber,1853


Tylopsis lilifolia Fabricius,1793
Leptophyes albovittata Kollar,1833
Isophya brunneri Retowskii,1888
Isophya taurica Brunner v.Wat.,1878
Poecilimon schmidti Fieber,1853
Poecilimon intermedius Fieber,1853
Poecilimo boldyrevi Mirat,1938
Poecilimon tauricus Retowskii,1888
Poecilimon pliginskii Miram,1929
Poecilimon bey-bienkoi Tarbinski,1932
Poecilimon kuznetzovi Miram,1929
Meconema thalassiba hopard,1922
Saga pedo Pallas,1771
Tettigonia viridissima Linnaeus,1758
Tettigonia caudata Chopard,1922
Anadrymadusa retovskii Adelung,1907
Paradrymadusa galitzini Retowskii,1888
Gampsocleis glabra Herbst,1786
Decticus verrucivorus Linnaeus,1758
Decticus albifrons Fabricius,1775
Montana Medvedevi Miram,1927
Platycleis intermedia Serville,1839
Platycleis escalerai Bolivar,1899

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233

.., ..

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Platycleis grisea Fabricius,1781
Platycleis affinis Fieber,1853
Tessellana vittata harpentier,1825
Tessellana tesselata harpentier,1825
Platycleis sepium Verrin,1854
Bicolorana bicolor Philippi,1830
Bicolorana roeselii Kagenbach,1882
Pholidoptera griseoptera De Geer,1773
Ph. pustulipes Fisch.-Wald.,1846
Conocephalus discolor Thunberg,1815
Conocephalus dorsalis Latreille,1804
Homorocoryphus nitidulus Scopoli,1786
Gryllus campestris Linnaeus,1758
Gryllus bimaculatus De Geer,1773
Acheta domestica Linnaeus,1758
Melanogryllus desertus Pallas,1771
Melanogryllus frontalis Fiber,1845
Melanogryllus burdigaleisis Latreille,1804
Tartarogryllus tartarus Uvarov,1934
Gryllomorpha dalmatina Ocskay,1832
Gryllomorpha miramae Medvedev,1933
Discoptila fragosoi Bolivar,1885
Pteronemobius neideni Fischer,1853
Pteronemobius sylvestris Borc.
Arachnocephalus vestitus Costa,1855
Myrmecophilus hirticoudus1846
Myrmecophilus tatarica Karavajev,1929
Melanogryllus bordigoleisis Latreille,1804
Oecanthus pellucens Scopeli,1763
Gryllotalpa gryllotalpa Linnaeus,1758
Xya variegatus Latreille,1809
Tetrix subulata Linnaeus,1761
Tetrix nutans Hagenbach,1822
Tetrix bipunctata Linnaeus,1758
Tetrix depressa Brissen,1848
Asiotmethis tauricus Tarbinski,1930
Podisma pedestris Linnaeus,1758
Anacridium aegyptium Linnaeus,1764
Calliptamus italicus Linnaeus,1758
Calliptamus barbarus Costa,1836
Acrida bicolor Thunberg,1815

2
+
+
+
+
+
+
+
+
+
+
+

234

3
+
++
+
+
+
+
+
+
+
+
+
+
+
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+
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4
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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1
5
6
7
8
+
+
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++
+
+
+
++
+
+
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+
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+
+
+
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+
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+
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+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
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+
+
+
+
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+
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+
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+
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+
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1
Chrysochraon dispar Germar,1835
Euthystira brachyptera Ocskay,1824
Paracinema tricolor Charpent,1825
Acryptera fusca Pallas,1773
Pararcyptera microptera F.-W.,1833
Pararcyptera microptera jailensis Mir.,1927
Ramburiell turcomana F.-W.,1833
Dociostaurus maroccanus Thunberg,1815
Dociostaurus brevicollis Evert.,1848
Stenobothrus lineatus Panzer,1796
Stenobothrus fischeri Evert.,1848
Stenobothrus miramae Dirsh.,1931
Stenobothrus eurasius Vor.,1928
Omocestus viridulus Linnaeus,1758
Omocestus ventralis Zetterstedt,1821
Omocestus haemorrhoidalis Charpent.,1825
Omocestus petracus Brisout-Barneville,1855
Myrmeleotettix maculatus Thnberg,1815
Stauroderus scalaris F.-W.,1846
Chorthippus biguttulus Linnalus, 1758
Chorthippus brunneus Ramme, 1939
Chorthippus millis Charpentier,1825
Chorthippus apricarius Linnaeus,1758
Chorthippus macrocerus Voronzovskij,1928
Chorthippus pullus Philippi,1830
Chorthippus vagans Everts,1848
Chorthippus longicornis Latrielle,1804
Chorthippus albomarginatus De Geer, 1773
Chorthippus dorsatus Zetterstedt,1821
Chorthippus loratus F.-W.,1846
Euchorthippus pulvinatus F.-W.,1846
Epacromius coerulipes Ivanov,1887
Aiolopus thalassinus Fabricius,1781
Aiolopus strepens Latreille,1804
Locusta migratoria Linnaeus,1758
Oedaleus decorus Germar, 1817
Celes variabilis Pallas.,1774
Oedipoda coerulescens Linnaeus,1758
Acrotylus longipes Charpentier,1845
Sphingonotus coerulans Linnaeus,1767
Sphingonotus djakonovi Mistshenko,1936

2
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3
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235

.., ..


, . ,

, . , ,


(13 15 ) (9 14 ),
- (1 1) (1 1) .

(4 14) - (5 9) .
(26,79%),
(14,83%) (12,92%),
: Tetrix Latreille,1802, Decticus Serville,1831, Pholidoptera Wermaeb,1838.

(22,72%), (20,45%)
, Platicleis Fieber,1852, Conocephalus
Thunberg,1815, Calliptamus Serville,1831 Stenobothrus Fischer,1853.

: 1,37, 1,40 ,
55,33%, 71,25%.

- , , - . ,
, .
-
.
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(21,43%),
(17,86%) (17,86%) : Poecilimon
Fieber,1853, Tartarogryllus Tarbinskii,1940 Calliptamus Serville,1831 ( 1).

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Conocephalus Thunberg,1815 Tesselana Leuner,1941.
17,86%.

, 13%.
(11) .
(4), - (4), -
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236

-
(
60%, - 75%).

.
- ,
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- .
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Fiebr,1852 ( 1). , ,
- .
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-
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(23,63%), (21,05%)
(13,16%). : Sphingonotus Fiebr,1853,
Celes Saussure,1884, Epacromius Uvarov,1942, Omocestus Bolivar,1879 ( 1).
(11 ), (8),
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, ,
(
47,82%, 64,7%).
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: (27,27%),
(20,45%) (13,64%),
: Tetrix Latreille,1802, Stenobothrus Fischer, 1853, Omocestus Bolivar,1879
Platcleis Fieber,1852 ( 1).
( 58,84%,
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237

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Paradrymadusa Herman,1874, Poecilimon Fieber, 1853 (. 1).
: (16 ),
(13), (12) (10).
: - (7 ), -
(5), - (5), - (4) .
(80 )
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(28,17%), (14,07%)
(9,86%), (12,68), (11,27%),
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, , ,
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. : (17 ),
(11), (11) (11). :
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.
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- (4) .
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(36,19%)
, ,
, .

238

, -
,
.

1.

..
/ .. //
. - .: , 1986. . 3-10.
2. .. (
) / .. - .: , 1978. 198c.
3. .. -
/ .. // . . .. , 1998. - 4. - . 445-450.
4. .. Orthoptera
/ .. // . - :
, 1988. . 15-26.
5. .. / .. // :
.- - .: - , , , 1993. . 29-49.
6. .. ,
/ .. // . ,1976.- . 55. . 11.
.1640-1647.
7. .. (Orthoptera)
/ .. // . , 1979.- . 58, . 1.
. 42-52.
8. .. : BisCrim / .., .., .. //
, .- : , 2003.- .13.-.184-187.
9. .. - : BisCrim /
.., .. // . - : . ,2004..17(56).- 2.-.156-164.
10. .. : CrimInsecta / .. ,
.. // -2004.-: ,
2004. .26-27
.. -i (Insecta, Orthoptera) /
.. i, .. //
. .. . , . 2011. . 24 (63), 2. . 231-239.
Criminsecta , , i
(Orthoptera) ii i i ,
, i .
: , , , , .
Pishkin V. Ecological- geograpic review of Orthoptera (Insecta, Orthoptera) of Crimea / V. Pishkin,
N. Visockaya // Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry.
2011. Vol. 24 (63), No 2. . 231-239.
Created within the framework of the program Criminsecta database on taksonomii, allowed ecology and
chorology of Orthoptera insects of Crimea to select the entomological complexes of fiziko-geographical
provinces and areas of peninsula, study their structure and forming features.
Keywords: Crimea, entomofauna, pryamokrylye, ecology, natural habitat, life-form.

11.06.2011 .

239

. . .
, . 24 (63). 2011. 2. . 240-248.

574.472:595.771:614.449.57



.., .., ..
- . .. ,
,
E-mail: rusevivan@ukr.net
. 13
. Aedes caspius (Pall., 1771), Culex pipiens (L.,
1758). Aedes vexans (Meig., 1830), Mansonia richiardii (Fic.,
1896), Anopheles maculipennis (Meig., 1804), Culex modestus (Fic., 1890),
Uranotaenia unguiculata (Edw., 19130) Anopheles hyrcanus (Pall., 1771).
(, ) Culex pipiens Culex pipiens pipiens f. molestus.
Culex pipiens pipiens f. molestus
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40 ,
.

240

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241

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20 .

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, Culiseta annulata.
100

Culiseta
annulata

(%)

90
80
70

Culex pipiens

60
50
40
30

Aedes caspius

20
10
0

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.
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.
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100

(%)

90

Culex pipiens

80

Anopheles maculipennis

70
60

Aedes caspius

50

Aedes vexans

40
30

Mansonia richiardii

20

Uranotenia unguiculata

10
0

Culex modestus

.2. ,

2006
4765 12 , 6
. , ,
,
Culex pipiens 887.4%, 62.69.9% 82.11.1%.

16.74.7%, .. .
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2 .
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(.3).
,
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243

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2006 .
Culiseta annulata
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244

1,6

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31,05

2,06

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.
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1% (.1.)
1

(% (95))

2005 .
2006 .
2007
Aedes cantans (Meigen, 1818)
0.20.2
0
0
Aedes caspius (Pallas, 1771)
4.00.8
11.70.9
1.70.7
Aedes cinereus (Meigen, 1818)
0
0.040.04
0
Aedes excrucians (Walker, 1848)
0
0.10.1
0
Aedes flavescens (Mller, 1764)
0
6.70.7
0.90.5
Aedes vexans (Meigen, 1830)
1.20.4
0.80.3
0.80.5
Anopheles hyrcanus (Pall., 1771)
0
0.10.1
0
Anopheles maculipennis
(Meigen, 1818)
0.20.1
0.040.04
0
Culex modestus
(Fic., 1890)
1.60.5
010.1
0.40.3
Culex pipiens (Linnaeus, 1758)
87.91.3
78.01.2
93.01.4
Culiseta annulata (Schranck, 1776)
4.30.8
1.30.3
0.80.5
Mansonia richiardii
(Ficalbi, 1889)
0.70.3
1.00.3
2.4+-0.8
Uranotaenia unguiculata (Edw.,1913)
0.10.1
0.10.1
0.2+-0.2

0.8137
1.1555
0.5586

245

.., .., ..

, 2005 ,
2006 , Culex pipiens
, .. . 2007
,
Culex pipiens 93% 87.9% 78% 2005
2006 . .

()
, 2006 2007 .
(98 %) Culex
pipiens 7362 .,
Aedes caspius 62 ., Mansonia richiardii 29 ., Culiseta annulata 19 ., Aedes
flavescens 11 ., Uranotaenia unguiculata 2 ., Aedes vexans 2 ., Anopheles
hyrcanus 1 . 6483 .
1005 . .
-
(-). 8 %
, , ,

. , ,
,
(t=3,720; p<0,001).
12 ,
, 10 Culex pipiens 3 Culiseta annulata.
, Culex pipiens

[8], [9]. , ,
2001-2002 . 1999- , ,
Culex
pipiens f. molestus, ,

[10].
. , ( )
.
,
Culiseta annulata,
. Culiseta annulata
, [11].
. , , Aedes caspius
Anopheles hyrcanus, -
. .

246

,
. ()

:
,
, , ,
, 1 % [5].
(
)
.

1. . 13
.
Aedes caspius Culex pipiens.
Aedes vexans, Mansonia richiardii,
An.maculipennis, Culex modestus, - Uranotaenia
unguiculata Anopheles hyrcanus.
2.
Culex pipiens pipiens
f. molestus. ,
,
, , .
3.
Culex pipiens pipiens f. molestus
,

.
. ,
, . ..
.

1.
2.
3.
4.

.. / ... - : .
. 1998. 34 .

/ .. -, .. , . [ .] // IX .
. : .. - , 1980. - . 1. - .121 - 122.
.. " ". /
.. // .: . .,1963. - 366 .
.. -
/ . . // . II . . . - , - ,
1956. -. 252-254.

247

.., .., ..
5.

Goddard J. Mosquito Vector Competence and West Nile Virus Transmission / J.Goddard //Infect Med. 2002. Vol. 19, 12. . 542 - 543.
6. .. . / .. ., .. , .. , ..
// - "
:17-19 2008 .;.. , 2008. .167 -169
7. .. , , , /
.. // . . . 1988. - .62 - 69
8. West Nile Virus isolates from mosquitoes in New York and New Jersey in 1999 / R.S., Nasci D.J.,
White H. Stirling [et al.] // Emerg. Infect. Dis. - 2001. -Vol.7, 4. - . 626 - 630.
9. Entomological and avian investigations of an epidemic of West Nile fever in 1996, with serologic and
molecular characterization of a virus isolate from mosquitos / H.M., C. Savage, N. Ceianu Karabatsos [et
al.] // Am. J. Trop. Med. Hyg. 1999. Vol. 61, 4. . 600 611.
10. (Diptera, Culicidae) .
1999 . / .. , .. ,
.. [ .] // . 2004. - 3. . 209-217.
11. .. - 2002 .:
/ .. , .. , .., .. //
. 2003. . 86. . 139-148.

..
/ .. , .. , .. //
. .. . , . 2011. . 24 (63), 2.
. 240-248.
. 13
. Aedes caspius Culex pipiens.. ,
Aedes vexans, Mansonia richiardii, Anopheles maculipennis, Culex modestus,
, Uranotaenia unguiculata Anopheles hyrcanus.
(, ) Culex
pipiens - Culex pipiens pipiens f. molestus.
Culex pipiens pipiens f. molestus ,

.
: , , ,
.
Rusev I.T. Complex of fauna of mosquitoes in urban biocenosis and they role in circulation of west nile
virus / I.T. Rusev, V.N. Zacusilo, V.D. Vinnik // Scientific Notes of Taurida V.I. Vernadsky National
University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . . 240-248
The article is devoted to the 13 species of mosquitoes, living in open urban biotopes in Odessa. Among all
mosquitoes was found 2 dominant species - Aedes caspius Culex pipiens. Species with low occurring was
Aedes vexans, Mansonia richiardii, Anopheles maculipennis and Culex modestus. Species with rare occurring
was Uranotaenia unguiculata and Anopheles hyrcanus. In underground and free rooms on the steps place of
the building 3 species of mosquitoes was found - Culex pipiens L., Culiseta annulata Schrk. Uranotaenia
unguiculata Edw. Culex pipiens L., Culiseta annulata Schrk. Uranotaenia unguiculata Edw. Buildings with
antisanitary conditions and wet undergrounds there are suitable biotops for mosquitoes and they breading
round all year. For the bites they are use human, rats and pigeons. Firstly in Ukraine in urban areas was found
ecological type of Culex pipiens C.p. pipiens f. molestus as vector of West Nile fever virus.
Keywords: mosquitoes, West Nile fever, ecosystems of parasites, urban areas.

17.06.2011 .

248

. . .
, . 24 (63). 2011. 2. . 249-253.

594:124:094.3(262.5)



..
. .., ,
E-mail: masterkristi@rambler.ru

. ,

, .
,
.
.
.
: , , , .

,
[1, 2].
,
, ,
. , ,
- [3],
[4],
[5, 6],
[7].

[8, 9]. ,
, ,

, , .


.

Mytillus galloprovincials
Lam. 3,54,0 .

249

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, .
(5 )
.
: Dunaliella salina Teod., Thalassiosira psaudonana (Hustedt) Hasle et Heimdal
Pavlova lutheri (Droop) Green.
0,1 1
; 1,0 -1, 10,0 -1. (3 ) ,
.
810.
,
. , ,
, : 0,1 -1; 1,0
-1, 10,0 -1, - .

,
. ,
,
, 0,1 -1; 1,0 -1, 10,0 -1.
,
, .
( m).
1 1 ,
[10].

:

. . 1.

. 1. D. salina (), Th. psudonana:


(), P. lutheri: 1 0,1 -1, 2 1,0 -1, 3
10,0 -1.
0,05

250

,
,
. ,
23 (0,05).

'
,
, .
,
.

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, dl2

1 3 .

(. 2).
, . 2,
.
.

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(. 2).

. 2. (), ()
()
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-1
.
0,05


.
(. 3, ).

251

..


.
.
( 1,0 -1) (10,0 -1).

0,05 (. 3, ).

. 3. (
() - D. Salina, () -
Th. Psudonana, () P. lutheri)
1 0,1 -1, 2 1,0 -1, 3 10,0
-1

0,05

.
0,05 (. 3,).



.

1.
.
.
2.
, .
3.
.
.

252


1.

. . : /
. . // . . 1999. . 35, 1. . 22-39.
2. .. / .. , . . .: , 1987. 285 .
3. Da Ros L. Biomarkers and trase metals in the digestive gland of indigenous and transplanted mussel
Mytyllus galloprovincialis in Venice Zagoon, Italy / L. Da Ros, C. Nasci, I. Marigomez, M. Soto // Mar.
Environ. Res. 2000. Vol. 50. P. 417- 423.
4. . . Mytilus trassullus /
. ., . . , . . // . 1998. . 24. 5. . 319325.
5. onesi L. Heavy metals and glutathione metabolism in mussel tissues/ L. onesi, A. Viarendo, C.
Leonzio, M. Filipelli, G. Gallo // Aquat. Toxicol. 1999. Vol. 46, 1. P. 67 76.
6. Khessiba A. Biochemichal response of the mussel Mytllus galloprovincialis from Birerta (Tunisia) to
chemical pollutant exposure / A. Khessiba, P. Hoaran, N. Gnassia-Barelli, P. Asissa // Arch. Environ.
Contan. Toxicol. 2001. Vol. 40. 2. P. 222-229.
7. Regoli F. Trace metals and antioxidant enzymes in gills and digestive gland at the Mediterranean mussel
Mytllus galloprovincialis / F. Regoli // Arch. Environ. Contan. Toxicol. 1998. Vol. 34, 1. P. 4863.
8. Romeo M. Mussel transplantation and biomarkers as useful tools for assessing water quality in the NW
Mediterranian / M. Romeo, P. Hoaran, G. Garello, M. Gnassia-Barelli, J. P. Girard // Environ. Pollut.
2003. Vol. 122. 3. P. 369 378.
9. Livingstone D. R. Antioxidant enzymes in the digestive gland of the common mussels Mytyllus edulis /
D. R. Livingstone, F. Lips, P. Garsia Martinez, R. K. Pipe // Mar. Biol. 1992. Vol. 12, 2. P. 265276.
10. . . . . : . 1990. 362 .
..
/ .. //
. .. . , . 2011. . 24 (63),
2. .249-253

. , ea
, .

. .
.
: , , , .
Semenova O.O. Study of cadmium accumulation in tissues of Black Sea mussel at the different ways of
organism entering / O.O. Semenova // Scientific Notes of Taurida V.Vernadsky National University. Series:
Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 249-253.
The compare of cadmium accumulation to the Black Sea mussels organisms depending on the way . - of intake
(with food and from water environment) have been done. The main quantity of this heavy metal were
accumulated in the leg and branchiae of mussel in condition of soluble state as opposed to the condition of
nutritional intake. The characteristics of cadmium accumulation and his content in the tissues practically did
not depend on the source of his entering organism of shellfish. In the condition of nutritional intake cadmium
was accumulated in the tissues of branchiae. There were no effect of cadmium satiation in the tissues of
hepatopancreas and leg of mussels.
Keywords: algae, the Black Sea mussel, satiation, cadmium.

11.06.2011 .

253

. . .
, . 24 (63). 20110. 2. . 254-260.

582.261.1:556.028 + 569.5(262.5)



..1, ..2, ..2
1

, ,
c . .. , ,
E-mail: inik48@inbox.ru
2


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(Tursiops truncates ponticus Barabash 1940).
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.
: , , ,
.


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[1].
,
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(. , . ), ,

Bacillariophyta [2, 3].
[4].

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, [57],

[8].

254

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, 0,30,8/,
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, [10] ,
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2025 %
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10 , (. 1).

. ,
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costatum 1.1109 /.
, 50210 /.
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,
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4 (. 1).
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1
( )

2
3

2
3

Bacillariophyta:
Synedra fasciculata (Ag.) Kutz.
Thalassionema nitzschioides
(Grun.)

9,6 105/2

Bacillariophyta:

Amphiprora sp.
Amphora sp.

Navicula Bory.

Nitzschia sp.
Stauroneis constricta (Ehr.) Cl.
S. fasciculata (Ag ) Kutz.
T. nitzschioides (Grun.)
Bacillariophyta:
Amphora sp.
Navicula Bory.
Nitzschia sp.

Thalassiosira parva Pr-Lavr.

Dinophyta:
Prorocentrum cordatum
(Ostenf.) Dodge
Glenodinium sp.
Bacillariophyta:
Achnanthes breviper Ag.
Nitzschia sp.
Sceletonema costatum
(Grev.) Cl.
Synedra sp.
S. fasciculata (Ag ) Kutz.

T. nitzschioides (Grun.)

Dinophyta:
Oxytoxum sp.
Gymnodinium sp.
Gyrodinium pinque Swery
(Schutt) Kofoid & Swery
Chrysophyta:
Coccolithus sp.
Bacillariophyta:

Navicula sp.

S. costatum (Grev.) Cl.


S. fasciculata (Ag ) Kutz.

T. nitzschioides (Grun.)

257

1,0106/

2.5
3,0104/

2.3109/

4.2109/

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, ,
[13].
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258


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.

1.

.. (Tursiops truncates ponticus


Barabash 1940) /
.. // : . . 6
. ., 11 15 2010 . : , 2010. 530 534.

259

.., .., ..
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.

.I. ii ii-i /.I , .. i, ..


//i 1 ' . . .: .: . , 1992. .402 403.
.. -
/ .. // i iii . 2002. - .2. .188 203
.. -
/ .. , .. , .. .: . ,
1992. 110 .
.. , . .1. / .. ,
.. , .. .: . , 1980. 680 .
Gewalt W. Ultraviolet sterilization of water in pool for tonina (Inia Geoffrensis). A preliminary report /
W. Gewalt // Aquatic Mammals 1977. V. 5, No 3. P. 69 71.
Van der Toorn J.D. A biological approach to dolphinarium water purification: I. Theoretical aspects /
J.D. Van der Toorn // Aquatic Mammals 1987. V. 13, No 3. P.83 92.
// 1974. 8. .9 12.
..
/ .. , .. , .. //
, 1981. 10. .120 123.
..
/ .. , .. // . . 1988.
21, 4. . 4 8.
- .. -
/ .. -,
.. // . . 1999 . 35, 3. . 71 76
.. // . .- 1979.15.- . 71 -76.
.. / .. .: . , 1972. 256.
- .
.: . , 1976. . 31 33.
..
/.. , .. // i iii . 2002. .2.
.208 214.
.. / .. .: , 1982. .43 80.

.. /
.. , .. , .. //
. .. . , . 2011. . 24 (63), 2. . 254-260.

,
(Tursiops truncates ponticus Barabash 1940). .
: , , ,
.
Smyrnova L.L. Species composition and distribution of algae in the pools with marine mammals /
L.L. Smyrnova, I.I. Babeech, S.N. Anninsckaja // Scientific Notes of Taurida V.I. Vernadsky National
University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 254-260.
A comparative study of species and quantitative compositions of dominant species and genera of algae
growing in natural and chlorinated seawater in pools with bottlenose dolphins (Tursiops truncates ponticus
Barabash 1940) was conducted. The seasonal dynamics of bacterial-algal microfouling biomass developed on
the walls of the pool with dolphins in natural seawater shows.
Keywords: bottlenose dolphins, pools for the content of marine mammals, natural seawater, chlorinated seawater.

18.05.2011 .

260

. . .
, . 24 (63). 2011. 2. . 261-267.

577.3

EFFECT OF ELECTROMAGNETIC FIELD WITH 8 Hz FREQUENCY


ON THYMOCYTES NUCLEUSES INJURY CAUSED BY NANOSTRUCTURED
SILICON AND HYDROGEN PEROXIDE
Sobko V.M., Martynyuk V.S., Shevchenko V.B., Protopopov M.V.
Kiev National Taras Shevchenko University, Kiev, Ukraine
E-mail: svitya@ua.fm
Electromagnetic fields of low frequency are known to modulate cells functioning both in physiological and
pathological conditions, on what their effective application in medicine is based. Using the reliable and
sensitive method of comet assay the nucleuses DNA damages were studied in the suspension of isolated rat
thymocytes after three hour incubation with nanostructured silicon, hydrogen peroxide and exposure to
electromagnetic fields, considering both individual and combined effects. After the comet assay analysis we
confirmed the results of previous research that indicated the DNA damages in cells after combined influence
of nanostructured silicon, 0.1 mM hydrogen peroxide and electromagnetic fields. It was found that the
exposure of the suspension of thymocytes to electromagnetic fields with frequency of 8 Hz combined with the
action of hydrogen peroxide and nanostructured silicon in the presence of light leaded to the increased number
of DNA breaks in nucleuses that might be due to a protective response to peroxide damage.
Keywords: apoptosis, electromagnetic fields of low frequencies, comet assay, single- and double-strand breaks
of DNA, hydrogen peroxide, nanostructured silicon.

INTRODUCTION

During the past few decades, research of the electromagnetic waves influence has
developed into specialized sub-disciplines, from basic physics to tumor biology and
experimental therapy. One of actual problems of modern biophysics is to evaluate the
possibility of electromagnetic fields of low frequency to induce and modulate (modify)
cell death [1]. Recently it was shown that the combined influence of 8 Hz frequency EMF
with the action of oxidative factors such as hydrogen peroxide and nanostructured silicon
in the presence of light reduces viability of thymocytes by increasing the number of
apoptic cells compared to control [2]. It was supposed that energy of photoexcited silicon
nanocrystals is transferred to the interacting cells molecular system and results in tissue
damage mainly through generating of reactive oxygen species (R.O.S.), which induce
oxidative damage and genetic instability. The combined influence of 8 Hz frequency EMF
with the action of hydrogen peroxide and photoexcited nanostructured silicon is
considered to induce physical, chemical and molecular damage to tissues leading to
genomic instability and can cause cell death, particularly apoptosis [3, 4]. Nevertheless
received results are not absolutely reconcilable and further research are indispensable.
As a model for studying the influence of electromagnetic waves of low frequency
range (8 Hz) on the processes caused by the action of silicon and hydrogen peroxide (0.1

261

Sobko V.M., Martynyuk V.S., Shevchenko V.B., Protopopov M.V.

mM) as an oxidative factors suspension of isolated thymocytes were selected because they
were not fully differentiated cells, characterized by greater instability of genome than
other cells, and lower activity of DNA reparation systems of single strand breaks, which
facilitates the activation of apoptosis while they were exposed to various factors. The use
of isolated thymocytes for analysis of apoptosis in vitro allowed morphological control of
thymocytes and to identify cells with fragmented chromatin and apoptotic bodies, whereas
in vivo they were quickly absorbed by phagocytes [5].
Since a reliable and sensitive method was necessary for our research the comet assay
was chosen. The comet or single-cell gel electrophoresis assay is now widely used as a
quick, sensitive and cheap method for measuring DNA stand breaks in eukaryotic cells for
investigation of genetic damage associated with exposures to potentially genotoxic agents.
The comet assay was first described by Ostling and Johansson (1984) and in its
independent modification by Singh et al. (1988). One great advantage of this method is
that it is possible to measure the level of single- and double-strand breaks of DNA in
individual cells. The comet assay detects very reliably the level of single- and doublestrand breaks of DNA at low levels of damages [6, 7, 8].
The aim of our present study was to investigate DNA damages induced by
monoinfluences of chosen factors and the combined influence of 8 Hz frequency EMF
with the action of oxidative factors such as hydrogen peroxide and nanostructured silicon
in the presence of light compared to control.
MATERIALS AND METHODS

Thymocytes were obtained from the thymus of Wistar rats with weigh about 120-150
g which were kept in standard vivarium diet. Dedicated thymus was grinded through the
filter of synthetic fibers ( = 0,1 mm) in buffer solution with following composition
(g\1): NaCl 6,796; KCl 0,274; CaCl2 0,288; NaHCO3 2,091; KH2PO4 0,299;
MgSO4 0,144; glucose 1,8; (pH 7.4). The number of cells was counted by light
microscope in the chamber of Horyayev using dye (0,4% solution of trypan blue). If the
membrane integrity has been compromised (dead cells), the cells absorb the dye and
appear blue. When the cell viability was less than 80%, the cells were discarded and a new
batch was started.
Incubation of thymocytes (2-4 106 cells / ml) was performed in a water thermostat
at 37C in a stationary medium RPMI-1640 with the addition of 2.05 mM glutamine.
Incubation was been carried out for 3 hours with light.
Samples were subjected to the magnetic field treatment, which was created by the
Helmholtz rings. Impulses were rectangular with different polarity. Frequency of the
magnetic field was 8 Hz whereas induction of the magnetic field was 25 T. Frequency of
the magnetic field was selected on the basis of its ecological and geophysical significance
[9]. Vector of induction generated by the magnetic field was parallel to vector of the
geomagnetic field. Investigated samples were set down in Helmholtz rings. Control
samples were in the conditions of the electromagnetic fields background commonly
appropriate to the laboratory (20-65 nT). To assess the reliability of the impact of the

262

EFFECT OF ELECTROMAGNETIC FIELD WITH 8 HZ FREQUENCY

electromagnetic fields of low frequencies we used Students t-test for independent


samples linked in pairs.
In this experiment we used the following conditions: control cell suspension, cell
suspension, which has been subjected to the effects of nanocrystalline silicon with the size
of pores and crystallites of 2-5 nm activated by light, the electromagnetic wave with
frequency of 8 Hz during incubation, with the addition of hydrogen peroxide to a final
concentration of 0.1 mM in the incubation medium, a combination of electromagnetic
interference with hydrogen peroxide one.
To estimate DNA damage comet assays was chosen because of its quickness and
sensitivity. The neutral version of comet assay was carried out based on the work of
Syvolob et al as follows: on the day of electrophoresis, an aliquot of 50 l freshly
prepared suspension of cells was mixed with 100 ml 1 % low- melting-agarose. 25 l of
the mixture was layered on top of an ordinary microscope slide precoated with 1 %
normal-melting -agorose, which was allowed to dry at room temperature protected from
dust and other particles. After low-melting-agarose was solidified in a refrigerator for 3-5
minutes, the coverslip was carefully removed and the slide was gently immersed in a
freshly prepared lysing solution (2.5MNaCl, 100mM EDTA, 10mM Tris-HCl (pH=8,0),
with 1 % Triton-100 added just before use). Lysis was carried out at least for 2 h [10].
This assay is based on the separation from supercoiled DNA of DNA loops
containing strand breaks (specically DSBs) that become free to migrate out of the
nucleus towards the anode during a neutral electrophoresis in a TBE buffer (0,089 M Tris,
0,089 H3BO3, 2 mM EDTA, pH=7,5). After lysis slides were washed away with
electrophoresis buffer and were incubated for 10 minetes. Single cell gel electrophoresis
was performed for 20 min under following conditions: 4oC, 1V/sm, 300 nA) [10].
DNA images were captured after staining with DAPI with a phluorescent
microscope. For quantication, the comets were classied into different categories. We
subdivided cell DNA damage into ve stages (04) according to the length and the
intensity of the comet tail as illustrated in Fig. 1. Stage 0 (no tail) and stage 1 (halo around
the nucleus) corresponded to cells without a signicant number of DNA strand breaks.
Stages 24 corresponded to a gradual increase in DNA damage. We measured the comet
score in 100 randomly selected cells per slide. Results were, rst, expressed as the
percentage of each stage of comets per slide. In a second step, a comet score was
calculated with the help of the programe CometScore, following a modication of Collins
method, as the sum of the percentage of each comet stage multiplied by n (from 0 to 4)
(Collins 2004). The scores were expressed in arbitrary units on a scale from 0 (all the
comets are in stage 0) to 400 (all the comets are in stage 4) [11].
RESULTS AND DISCUSSION

The experiment designed to measure DNA damage was performed with rat
thymocytes suspension and showed nuclear DNA damage in samples exposured to
different factors and their combinations. By using scoring method of Collins (2004), the
DNA damage due to different influences shows different responses with certain regularity
(Table 1, Fig. 2). Sufficient discrimination about the degree of damage in DNA according
to comet appearance [Type 0 (no tail) to type 4 (almost all DNA in tail)] was given by

263

Sobko V.M., Martynyuk V.S., Shevchenko V.B., Protopopov M.V.

Collins (2004) (Fig.1.). A total of 100 comets were scored from each sample and each
comet assigned a value of 0 to 4 according to its type. The total score for the sample gel is
given between 0 to 400 arbitrary units. Most of the comets of unexposed samples
belonged to type 0 and type 1(Table 1, Fig. 2). The frequency of type 2, 3 and 4 increased
with respect to the increase of damaging impact strength to thymocytes, which further
correspondingly effects the arbitrary unit, showing increase from 31.0 in control sample to
178.0 in sample exposed to the combined effect of nanoporous silicon with H 2O2 and 8 Hz
frequency EMF.

Stage 0

Stage 1

Stage 2

Stage 3

Stage 4

Fig. 1. Different stages of DNA damage measured by comet assays.


Some baseline damage is always there which may be because of unwanted exposure
to so many environmental mutagens in day to day life and partially may be due to
processing error. The data presented in Figure 2 and Table 1 indicate that after tree hour
exposure the monoinfluence of electromagnetic field (EMF) with frequency of 8 Hz,
silicon and hydrogen peroxide increase the level of DNA damages in different ways. The
largest augmentation of DNA damages was observed under action of hydrogen peroxide,
namely 132, driven by oxidative injuries to cells. Under the monoaction of
electromagnetic field (EMF) with 8 Hz frequency insignificant increase of 37 arbitrary
units was observed. When nanostructured silicon was activated by light, the degree of
DNA damages amounts 40, which might be due to the formation of reactive oxygen
species.
Table 1
Score of DNA damage measured by comet assay in thymocytes immediately after
three-hour exposure to different factors and their combination.
DNA damage was in neutral conditions (DSBs). Data are expressed as arbitrary units
on a scale from 100 to 400; TC total number of cells scored. The experiment was
carried six times, and the average values presented in the table. Statistical analyses
were made by comparison of control values and values obtained after treatments
Sample

Factor of exposure

TC

Cells with
comet

1
2
3
4
5
6
7

control
n.s.silicon
8 Hz
H2O2
n.s.silicon+8 Hz
n.s.silicon+H2O2
H2O2+8 Hz

100
100
100
100
100
100
100

21
28
30
76
37
93
88

264

Comet type
0
79
72
70
24
63
7
12

1
11
18
24
42
26
48
50

2
10
8
5
23
9
27
23

3
0
2
1
7
1
12
10

Arbitrary unit
4
0
0
0
4
1
6
5

311,75
401,83
372,08
1322,58
512,25
1622,42
1461,58

EFFECT OF ELECTROMAGNETIC FIELD WITH 8 HZ FREQUENCY


8
Total

n.s.silicon+H2O2+8 Hz

100
800

98
471

43

38

1782,5

In some cases a significant enhancement of DNA damages level was observed when
we combined different factors of influence. After the combined action of 0.1 mM H2O2
and silicon the amount of DNA damages is 162; after the combined action of EMF with 8
Hz frequency and silicon the number of DNA damages makes 51 arbitrary units; the
combined influence of H2O2 and EMF augment the level of DNA damage, which is 146;
the combined influence of silicon, H2O2 and EMF with 8 Hz frequency has the strongest
impact on DNA damages, whish amounts 178 arbitrary units. It should be mentioned
that in all samples nanostructured silicon was activated by light, thus, it could efficiently
transfer their energy to the molecules of O2 adsorbed on the surface of nanocrystals. As a
result, the transition of the molecule from the triplet to the singlet state was observed and
nanoporous silicon could demonstrate oxidative effect [12]. Therefore, comparing the
results we can conclude that the amount of DNA damages was considerably increased by
influence of H2O2 and photoactivated silicon due to its oxidative properties and the effect
was enhanced by the EMF with frequency of 8 Hz, which is known to enhance oxidative
damages [13].
200
180

Arbitrary unit

160
140
120
100
80
60
40
20
0
1

Factor of exposure

Fig. 2. DNA damage in thymocytes after three-hour exposure to different factors and
their combination expressed as arbitrary units on a scale from 100 to 400.
1- control; 2 - n.s.silicon; 3 - 8 Hz; 4 - H2O2; 5 - n.s.silicon+8 Hz; 6 - n.s.silicon+H2O2; 7 H2O2+8 Hz; 8 - n.s.silicon+H2O2+8 Hz.
Overall it should be noted that the mechanisms of biological effect of 8 Hz frequency
EMF have been insufficiently researched, but despite this fact, magnetic fields have been
used quite effectively in modern medical practice. In particular they have been used for

265

Sobko V.M., Martynyuk V.S., Shevchenko V.B., Protopopov M.V.

anti-inflammatory therapy, accelerating tissue regeneration and improvement of


microcirculation. The generation of singlet oxygen caused by illumination of ensembles of
silicon nanocrystals was proposed to be used for the suppression of reproduction of cancer
cells. Presumably the suppression efficiency would be higher at the combined impact of
nanostructured silicon and EMF of 8 Hz frequency. It was believed that the physical basis
of this action was to coordinate the motion of charged particles [14]. The result of this
interaction was primarily the change in membrane potential and activity of lipid
peroxidation. In addition, the magnetic field affected the physical and chemical properties
of water, free-radical chemical reactions, macromolecule of large anisotropic diamagnetic
compounds [14].
CONCLUSION

The amount of DNA damages was considerably increased by influence of H 2O2 and
photoactivated silicon due to its oxidative properties and the effect was enhanced by the
EMF with frequency of 8 Hz, which is known to enhance oxidative damages.
ACKNOWLEDGEMENTS

The authors are sincerely grateful to the Department of Genetics of Taras Shevchenko
National University of Kyiv for providing laboratory facilities. The help rendered by
Afanasieva K.S. and Zazhytska M. are thankfully acknowledged.
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/ ., ., ., . // . . .
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/ .. // - 1991. . 1. - . 6-11.

.. 8 ,
/ .. ,
.. , .. , .. //
. .. . , . 2011. . 24 (63), 2. .261-267

, ,
.

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10.02.2011 .

267

. . .
, . 24 (63). 2011. 2. . 268-278.

615.849.11:595.123:57.034



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, .
, ,
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.
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.
4. 8 ,
, ,
.

1.

.. / .. //
( . ..). ., 1979. . 836.
2. .. /
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.. // . 1982. . 43. . 166173.
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14. ..
/ .. , .. , .. //
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/ .. , .. , .. //
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, 1982. .2. . 555643.
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/ .. , .. ,
.. // 1997. .4, 4. . 5660.
24. 98095 51 01 61/00.
/ .., .., ..;
. ...
U 200908540; .: 13.08.2009; . 10.03.2010, .5.
25. .. Dugesia tigrina
: . . . . . : . 03.00.13
/ .. . , 2010. 20 .
26.
/
.. , .. , .. [ .] //
... . . . 2010. .23, 2. . 125.
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.: , 1986. 160 .
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1976. 127 .

277

.., .., .., ..


31. .. -
Excel / .., .., .. : , 2000. 319 .
32. ..
/ .. // . 1995. . 40,
5. . 925927.
33. .. -
/ .. // . 1998. .43, . 5. . 789796.
34. ..
: . . . . : 03.00.02
/ .. , 1992. 15 .
35. ..
/ .. , .. //
. 1990. 2. . 191193.
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. . . . 1995. . 236238.
..
Dugesia tigrina / .. , .. ,
.. , .. //
. .. . , . 2011. . 24 (63), 2. . 268-278.
Dugesia tigrina.
,
, ,
. , 14-
-. ,
.
: Dugesia tigrina, , ,
, .
Temuryants N.A. The effect of weak electromagnetic factors in ultradian rhythms of locomotor activity
of planarians Dugesia tigrina / N.A. Temuryants, V.S. Martynyuk, N.S Yarmolyuk, A.V Shehotkin //
Scientific Notes OF Taurida V.Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63),
No 2. . 268-278.
Revealed ultradian rhythms speed planarian Dugesia tigrina. In a weak electromagnetic shielding, the action
of weak alternating magnetic fields of extremely low frequency of observed changes, reflected in pronounced
modifications of the power spectra, phase shift and amplitude of the selected periods. At first, seventh and
14th day of exposure to electromagnetic factors, these changes are expressed in different ways. Maximum
changes occurred on the first day of observation, and further periods of ultradian rhythm to normal.
Keywords: planarian Dugesia tigrina, speed, ultradian rhythm, alternating magnetic field of low frequency,
electromagnetic screening.

12.06.2011 .

278

. . .
, . 24 (63). 2011. 2. . 279-283.

528.675.1:57.086.83


CLEMATIS VITALBA ,

. ., .., .., ..
. .. , ,
E-mail: zayatc.alex@i.ua
Clematis vitalba L.
, .
. (13,52)
, 10 Mn /3.
: Clematis vitalba, , .


. ,
[1].

:
-
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- [2].

,

[3, 4].
.
,
, .

[5].

279

. ., .., .., ..


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,
[6].
7-10
( 1/28) (. 1), (II),
1 10 Mn/3,
, 25
65-70 %.

.
1.

C. vitalba

, /
NH4NO3
500
KNO3
2500
(NH4)2SO4
134
CaCl22H2O
150
MgSO47H2O
250
NaH2PO42H2O
300
Na2EDTA2H2O
37,3
FeSO47H2O
28,0
MnSO44H2O
13,2
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2,0
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3,0
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0,025
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0,25
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0,025
KI
0,75

0,25
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0,05
-Cl
0,25

10,0

50000
-
7000-8000

Microsoft Office.

280

, ,
( )
: - ;
;
8 . ()
3,62 (. 1).

. 1. ,

,
, .
, , 1,0 Mn /3, -
1 , 2 8 ,

.
, , 5,0 Mn /3 , -
-2 , 3 9 ,
9 .
, , 10,0 Mn /3, -
-1 , 2 8 ,
9 .
, ,
, , 1 Mn/ 3
4,34; 5 Mn/ 3 7,08 10 Mn/ 3 13,52 (. 2).

281

. ., .., .., ..

. 2. Clematis vitalba
:
1 ( Mn); 2 1,0 Mn /3; 3 5,0 Mn /3; 4 10,0 Mn /3

1. Mn
.
10 Mn/ 3 13,52.
2. Mn
54-64 , ,
7 (50 ),
.

1.
2.
3.

.. / ..
// , . -. 2001 .7, 8 .13-19
. .. . ./ .. .:
.- 2005. 724 .
. . : 3- . / [. .
, . . , . . .]. .: , 2001 2002. . 1. 2001. .
103, 105, 113. . . :
3- . / [. . , . . , . . .]. .: , 2001 2002. . 2.
2002. . 214, 281, 263.

282


4.
5.
6.

. . : .
. . . . . . : . 02.00.10 / . .
, 1974. 18 .
.. / .. . : , 1999 230 .
.. / .. ,
.. , .. . .: . , 1986. . 3436.

.. Clematis vitalba
, . / .. , .. , .. , .. //
. .. . , .
2011. . 24 (63), 2. . 279-283.
Clematis vitalba
, . ,
.
(13,52) , 10 Mn / dm3.
: Clematis vitalba, , .
Tepltitskaya L. Growth characteristics of callus cultures Clematis vitalba on cultures media containing
selenium and manganese. / L. Teplitskaya, A. Zayatc, I. Yurkova, A. Sidyakin // Scientific Notes OF Taurida
V.Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 279-283.
The growth characteristics of callus cultures of Clematis vitalba on the modified solid media of Gamborg and
Eveleg supplemented with manganese were under study. It was established that the growth index depends on
the concentration of manganese in the solid media. The maximum growth index (13.52) was observed on the
solid media containing 10 mg Mn / dm3.
Keywords: Clematis vitalba,callus culture, manganese.

12.06.2011 .

283

. . .
, . 24 (63). 2011. 2. . 284-290.

582.736.3:057.086.83/88


(MELILOTUS OFFICINALIS (L.) PALL.)

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. .. , ,
E-mail: vanabis@list.ru

Melilotus officinalis.
.
.
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.
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,
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. ,
in vitro , ,

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[3].

284

. .
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.


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, .

.
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.

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.

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2. ,
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3. ,
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1.
2.
3.
4.
5.
6.
7.

. . / . . .
.: , 1964. 272 .
. . /
. . // : [. . . . . ]. .: ,
1981. 37 50.

/ .., .., .. [ .] //
. - 1996. - 4. - . 253-258.
.. / ..,
.., .. . : , 1980. 488 .
: [. .] / , - . . . , . .
. . . . : , 1981. 168 .
. : [. . . .. ]. .: , 1965.
352 .
. . / .. . .: , 1970. 225 .

.. (Melilotus officinalis (L.) Pall.)


. / .. , .. , .. , .. //
. .. . , . 2011.
. 24 (63), 2. . 284-290.

Melilotus officinalis.
. ,
.
: Melilotus officinalis, , .
Tepltitskaya L. Receipt of callus cultures of M. officinalis and them cytomorphological features. /
L. Teplitskaya, I. Yurkova, A. Sidyakin, I. Zhupanov // Scientific Notes OF Taurida V.Vernadsky National
University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 284-290.
The features of callus induction in the culture of vegetative and generative organs of Melilotus officinalis
were investigate .Modifications of nutrient soil of Murashige and Skoog (MS) for callus induction were
chosen. Cytomorphological analysis of transplanted callus cultures was carry out. Its morphogenetical
potential low was exhibit.
Keywords: Melilotus officinalis,callus culture, cytomorphological analysis.

12.06.2011 .

290

. . .
, . 24 (63). 2011. 2. . 291-297.

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. . / . . //
. 2006. . 52. . 335345.
..
: . . . . . : .
03.00.04. / ;
. , 1992. 32, [1] ., . : . 12.
/ .. , ..
, .. [ .] // . . . 2001. . 73, 2. . 5156.
Thiamine triphosphate and thiamine triphosphatase activities: from bacteria to mammals / I. E. Gulyai,
A.F. Makarchikov, B. Lakaye [et al.] / Cell. Mol. Life Sci. 2003. V. 60. P. 1477 1148.
. .
/ .. // . 1998. 6. . 1114.
.. -L-
/ .. , .. // . . . 2004. 2. . 5660.
.. , / . .
// . . . 1986. . 58, 4. . 100 111.
. . / . . , . . //
. 2008. . 73. . 317.
..
/ .. , .. , .. //
. 2009. . 55. . 8997.
.. L . / .. // .
. . 1998. . 70, 5. . 97103.
Pfizer J.M. Primed-site Probing of Papain-like Cysteine Proteases / J.M. Pfizer, I. Assfalg-Machleidt,
W. Machleidt [et al.] // International Journal of Peptide Research and Therapeutics formerly known as
Letters in Peptide Science. 2006. P. 4467.
.. / .. ,
.. , .. // . . 70. 2005. . 416429.
. . /
. . , .. , .. // . . . 2003. . 75, 6. .
111114.
. 46633 , (2009), 12 N 9/50, 12 N 9/64.
-2 / .. ; .. u
2009 08087 ; . 31.07.2009 ; . 25.12.2009, . 24.
/ . . . . 2- ., . .
. : . , 1980. 272 .
Protein measurement with the Folin phenol reagent / O. H. Lowry, N. I. Rosenbrough, A. Z. Fan [et al.]
// J. Biol. Chem. 1951. V. 193. P. 265275.

296

L
.. L /
.. , .. , .. , .. //
. .. . , . 2011. . 24 (63),
2. .291-297

L. ,
L ,
.
: , ,
L.

Ustjansky O.V. Cathepsin L Activity Adjusting by Thiaminum and Its Derivatives / O.V. Ustjansky,
I.I. Bokal, O.V. Shvartcova, S.A. Petrov // Scientific Notes of Taurida V.Vernadsky National University.
Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 291-297.
In the article the influence of Thiaminum and some its metabolites on the activity of the cleared preparation of
cathepsin L are studied. It is determined that free Thiaminum and thiochrome can serve as effectors for the
cathepsin L by the specific joibing to it on a thioldisulfide mechanism, that results in activation of enzyme.
Keywords: Thiaminum and some its metabolites, sulphurcontained compounds. cleared preparation of
cathepsin L.

23.05.2011 .

297

. . .
, . 24 (63). 2011. 2. . 298-303.

616-007.7


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1.

Johnson D.E. Medical and developmental sequelae of early childhood institutionalization in Eastern
European adoptees / D. E. Johnson // Minnesota Symposia on Child Psychology. 2000. 31. P.
113-162.

302


2.

Frank D.A., Klass P.E., Earls F. Infants and young children in orphanages: One view from pediatrics and
child psychiatry // Pediatrics. 1996. V. 97. P. 569-577.
3. Juffer F., Van Jzendoorn M.H. Behavior problems and mental health referrals of international adoptees:
A meta-analysis / F. Juffer, M. H. Van Jzendoorn // J. American Medical Association. 2005. V. 293
(20). P. 25012515.
4. Smyke A.T. Attachment disturbances in young children. I: The continuum of caretaking casualty / A. T.
Smyke, A. Dumitrescu, C. H. Zeanah // J. American Academy of Children and Adolescent Psychiatry.
2002. V. 41. P. 972982.
5. Zeanah C.H. Attachment in institutionalized and non-institutionalized Romanian children / C. H. Zeanah,
A. T. Smyke, S.F.M. Koga [et al.] // Children development. 2005. V. 76. P. 10151028.
6. Karel S.R., Freeman B. J. Analysis of environmental deprivation: Cognitive and social development in
Romanian orphans / S. R. Karel, B. J. Freeman // J. of Child Psychology and Psychiatry. 1994. V. 35
(4). P. 769-781.
7. / . . . . : - , 2004 . 510 .
8. Albers L. H. Health of children adopted from the former Soviet Union and Eastern Europe. Comparison
with preadoptive medical records / L. H. Albers, D.E. Johnson, M. K. Iverson [et al.] // J. American
medical association. 1997. V. 278. P. 922924.
9. Ijzendoorn Van M. H. Plasticity of growth in height, weight, and head circumference: meta-analytic
evidence of massive catch-up after international adoption / Van M. H. Ijzendoorn, F. Juffer // J. Dev
Behav Pediatr. 2007. V. 28(4). P. 33443.
10. Blizzard R. M. Psychosocial short stature: a syndrome with many variables / R.M. Blizzard , A.
Bulatovic // Baillieres Clin Endocrinol Metab. 1992. V. 6(3). P. 687712.
11. Grantham-McGregor S. M. Effect of health and nutrition on cognitive and behavioral development in
children in the first three years of life. Part 2. Infection and micronutrient deficiencies: iodine, iron and
zinc / S. M. Grantham-McGregor, L.C. Fernald, K.Sethuraman // Food and nutrition Bulletin. 1999.
V. 20. P. 76-99.
.. 24 / .. ,
.. , .. , .. , .. //
. .. . , . 2011. . 24 (63), 2.
. 298-303.
, , ,
,
. , , ,
'
. ,
.
: , , , , .
Fokina Iu.O. The dynamics of orphans physical development under 24 months of age / Iu. O. Fokina,
O.M. Kochuhova, I.Ya. Timush, E.S. Vistman, V.B. Pavlenko // Scientific Notes OF Taurida V.Vernadsky
National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 298-303.
Institution-reared children have physical growth delays (weight, height and head circumference)
comparatively with normative anthropometric data. Physical growth delay of institution-reared children
directly relates with birth physical growth delay and childs age when he/she entered at the institution. The
later children enter at the institution, the more delay they have.
Keywords: physical development, weight, height and head circumference, children's home.

12.03.2011 .

303

. . .
, . 24 (63). 2011. 2. . 304-312.

612.014.46

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1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
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17.
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.. / ..
// .-. . 1994. . 28, 2. . 4-8.
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, 2002. . 1: . 2002. 540 .
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. 1998. 224 .
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1998. 332 .
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1993. 576 .

/ .. , .. , .. [ .] // II
, (), , 29-31 2008, . 74.
.. ,
/ .. , .. , .. [ .] //
, , . 2010. . 23. 2. . 193 199.
..
/ .. , .. , .. // /
Neurophysiology. 2005. . 37, 2. . 142-150.
..
/ .. , .. , .. //
/ Neurophysiology. 2006. . 38, 1. . 11-18.
..
/ .. , .. , .. [ .] // ,
, . 2009. . 22. 1. . 25-31.
Berridge M.G. Neuronal calcium signaling / M.G. Berridge // Neuron. 1998. Vol. 21, 1. . 13
18.
.. / .. .: , 1986. 256 .
.. / .. .:
. , 1981. 208 .

/ .. , .. , .. [ .] //
. . 1987. . 4, 9. . 932 936.
.. / .. , .. //
. . 2002. . 33, 4. . 3-33.
Meir. A. Ion channels in presinaptic nerve terminals and control of transmitter realease / A. Meir, S.
Ginsburg, A. Butkevich and oth. // Physiol. Rev. 1999. V. 79, 3. P. 1019 1088.
..
/ .., .. , .. [.] //
, , . 2010. . 1, 23. . 113-118.
Kramer R.H. Calcium-induced inactivation of calcium current causes the interburst hyperpolarization of
Aplysia bursting neurons / R.H. Kramer, R.S. Zucker // J. Physiol. 1985. Vol. 362, 2. P. 131-160.
.. 1164229 . ` ,
/ .. , .. () 1164229; .
29.11.2004 .
Kato Chemical characteristics of the L-glutamate receptor on the Onchidium neuron / Kato, Y. Oomura,
J. Maruhashi [et al.] // J. Neurosci. 1983. Vol. 3, 3. P. 549-556.
..
/ .. , .. // / Neurophysiology 1989. .
21, 6. . 820-826.

311

.., .., .., .., ..


23. ..
/ .. , .. //
/ Neurophysiology 1983. . 15, 3. . 2047-2054.
24. .. / ..
, .. // , , . 2000. . 2,
13. . 34-41.
25. ..
/ .. , .. // /
Neurophysiology 2001. . 33, 1. . 46 54.
26. .. / ..
// . . . 2006. . 6, 1-2. . 1139-1155.
27. ..
/ .. // . . . 2007. . 7, 3-4. .
2121-2130.
28. .. / .. , .. / : .
, 2003. 84 .
29. Jabalpurwala K.E. Metal ligand stability constants of some ortho-substituted phenols / K.E. Jabalpurwala,
K.A. Venkatachalam, M.B. Kabadi // J. Inorg. Nucl. Chem. 1964. Vol. 26, 6, P. 1027-1043.
..
/ .. , .. , .. , ..,
.. // . .. .
, . 2011. . 24 (63), 2. . 304-312.

`
. ,
,

.
: , , , ,
.
Cheretayev I.V. Influence salicylic acid and their salts on impulsive activity neurons of snail t the
blockage a calcium system / I.V. Cheretayev, D.R. Husainov, I.I. Korenjuk, O.V. Katjushina,
T.V. Gamma // Scientific Notes OF Taurida V.Vernadsky National University. Series: Biology, chemistry.
2011. Vol. 24 (63), No 2. . 304-312.
The article is about results of researches of neurotropic effects of acetylsalicylic acid and acetylsalicylates
cobalt and zinc It was made attempt to find their mechanisms from a state of calcium system snail`s neurons.
On experiments with blockade of an entering calcium current by cadmium chloride and with the blockade of
an entering calcium current and allocation of calcium from intracellular depot of barium chloride it is shown
that extracellular and intracellular ions of calcium do not participate in neurotropic effects of acetylsalicylic
acid and its salts.
Keywords: neurons of mollusc, acetylsalicylic acid, acetylsalicylates, calcium ions, blockers.

12.05.2011 .

312

. . .
, . 24 (63). 2011. 2. . 313-320.

582.675.1.086.83.:547.91

FATSHEDERA LIZEI

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.

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, ,
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-
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Fatshedera lizei ,
(.1).

, (95,02,1)
MC7, 2,0 / 2,4-; 0,5 /
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,
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51,23,5
80,13,3
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88,34,3
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.
, , ,
,
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315

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. ,
,
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2
3


, /

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6-

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. .

316

FATSHEDERA LIZEI

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, .
.
.

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.

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, , , ,
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.

317

.., .., .., .., ..

. 3.
Fatshedera lizei.
1 ,
6;
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3c ,
7.

12
28

OH

OH

HO
H

R2

R1

1, 2

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3, 4

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H

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2, 4

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,
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318

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,

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.

, . ,


Ginkgo biloba L., Atrogene sibirica L., Ycca macrocarpa Englem, Dioscorea deltoidea
W., Clematis vitalba L., Nerium oleander L. ,
, ,
.
Fatshedera lizei
,

,
,
.

1.
Fatshedera lizei.
2. ,
.
3. ,
Fatshedera lizei ,
.

1.
2.
3.

4.

5.
6.

.. . - /
.. . . : , 2005. 730 .
. . / .. //
. . : , 1991. . 520.
. I. i ii i: i, , ii
i i : . ... - i. : 02.00.10 /
I; i-ii i i. .. . , 2004.
39 .
. Fatsia japonica,
: . . . . :
15.00.02 , / ; , -
. , 1999. 26 .
. . / .. ,
.. // . . 1975. . 2, 11. . 27.
Murashige T.A revised medium for rapid growth and bioassays with tobacco tissue cultures / T.
Murashige, F. Skoog // Plant Physiol. 1962. V.15. P. 473497.

319

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7.
8.

.. / .. ,
.. , .. . . : , 1980. 488 .
: [. .] / , - . . . , . .
.. . . : , 1981. 168 .

.. i Fatshedera lizei - ii /
.. , .. , .. , .. , .. //
. .. . , . 2011.
. 24 (63), 2. . 313-320.
Fatshedera lizei.
.
.
,
.
: Fatshedera lizei, , .
Chmeleva S.I. Callus cultures Fatshedera lizei - producers of triterpene glycosides / S.I. Chmeleva
T.Y. Branovitskay, D.A. Panov, A.V. Omelchenko, I.A. Bugara // Scientific Notes of Taurida
V.I. Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No 2. . 313-320.
The features of the induction of callus induction in the culture of the vegetative organs Fatshedera lizei.
Podobranny modification of culture media Murashige and Skoog medium for callus induction. Passiruemy
callus has a high level of morphological heterogeneity. Experimental evidence of the presence of callus
cultures of different fractions of triterpene glycosides similar to intact plants.
Keywords: Fatshedera lizei, callus culture, triterpene glycosides.

26.05.2011 .

320

. . .
, . 24 (63). 2011. 2. . 321-331.

616.12.-008.318:613.614.2





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1.
2.
3.

.. / .. , .. , .
, .. / . 2009. 3 34-36 .
. .
Application of Low-Intensity Millimeter Waves in Biology and Medici /. . , .. /
2007. 8. 6-15 .
.. ,
/ .. , .. , . /
. 1998. .5, 2. 15-17 .

329

.., ..
4.

5.

6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.

..

/ .. , .. , .. /
, . 1988. 3. 14-18 .
..
/ .. , .. , .. /
. , 1994. 210213c.
.. - / ..
, .. , .. / . .:
, 1995. 31-33 .
. .
: . . / . . , . . / : ,
1989. 40 .
.. . / .. / -: , 2007 138 .
..
/ .. , .. / . 2001. . 27 2. 91-97.
..
/ .. , .. , ..
/ - . 2008. .11, 2. 18-31.
: ,
/ . - - // . 1999. 11. 53-78 .
..
: . . / . . , . . / : ,
1989. 40 .
.. / .. , .3. / :
1984 304 .
:
. . : , 2003. 24 .
Andersen K. Fundamentals of exercise testing / K. Andersen, R. Shephard, H. Denolin / Geneva, 1979.
320 .
.. : . / .. /
: . . . , 2002. 290 .
..
/ .. , .., .. /
, . 17, No2, 2009. 206-213 .
.. / .. , ..
/. .: , 1984. 220 .
..
( ) / .. , .. , ..
/ . 2001. . 24., 66-85 .
.. : . -
. . ., 2001. 12 c.
.. / ..
.. / .: - , 2001. 96 .
..
/ .. , .. , ..
/ . 2006. 1. 2-4 .
Richter D. W., Spyer K. M Central regulation of autonomic functions. / Cardiorespiratory control NY:
Oxford Univ. Press, 1990. 189-207 .
.. / ..
, .. , .. , .. / . . , 2006.
35., 11-15 .

330


25. ..
: : . .
, 004. 417
..
/ .. ,
.. // . .. .
, . 2011. . 24 (63), 2. . 321-331.
30-

( )
,
,
.
: , ,
, .
Chuyan E.N. Anti-inflammatory activity of salicylic and acetylsalicylic acids and its salts / E.N. Chuyan,
.R. Nkforov // Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry.
2011. Vol. 24 (63), No 2. . 321-331.
Changes of heart rate variability during the 30-minute recovery period after submaximal exercise in subjects
under the influence of low-intensity electromagnetic radiation of extremely high frequency (EMR EHF).
It is shown that the preventive effect of a ten EMR EHF has a marked antistress action in response to
submaximal exercise, as well as to increase the speed of recovery.
Keywords: heart rate variability, extremely high frequency radiation, veloergometricheskaya sample, the
recovery period.

07.06.2011 .

331

. . .
, . 24 (63). 2011. 2. . 332-338.

591.5:612.014.46



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E-mail: katsea87@mail.ru

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. . :
/ . . //
. 1997. . 106, 35. . 4.
. .
/ . . // . 2000. .2,
1 . 17-22.
Sodium naproxen: concentration and effect on inflammatory response mediators in human rheumatoid
synovial fluid. / P. Bertin, F. Lapique, E. Payan [et al] // Eur. J. Clin. Pharmacol. 1994; 46: 3-7.
. . / . . // . 1996. 10.
- . 2832.
:
/ [ . ., . ., .
., ..]. : , 1988. 240 .
. . - /
. . , . . // . . . 1998. . 6, 13 . 829-831.
Chan F. K. Celecoxib versus diclofenac and omeprazole in reducing the risk of recurrent ulcer bleeding in
patients with arthritis./ F. K. Chan // N. Engl. J. Med. 2002. V. 347. - P. 2104-2110.

337

.., .., .., .., .., ..,


..
10. Dahlen B. Celecoxib in patients with asthma and aspirin intolerance / B. Dahlen // N. Engl. J. Med.
2001. V. 344. P. 142.
11. Mogilevskaya E., Demin O., Goryanin I. Kinetic model of mitochondrial krebs cycle: unraveling the
mechanism of salicylate hepatotoxic effects / E. Mogilevskaya, O. Demin, I. Goryanin // Journal of
Biological Physics. 2006. - V. 32, 3-4. . 245-271.
12. . 1793578 . ,
/ .., .., .. . ; .
1993; . 1996, . 27.
13. . . ()
/ . . .: , 2005. 832 .
14. Dubuisson D. The formalin test: a quantitative study of the analgesic effects of morphine,meperidine and
brainstem stimulation in rats and cats / D. Dubuisson, S.G. Dennis // Pain. 1997. V. 4. . 161-164.
.. /
.. , .. , .. , I.I. , .. , .. ,
.. // . .. .
, . 2011. . 24 (63), 2. . 332-338.

() () () ().
, ,
.
: , , , ,
, .
Jakovchuk T.V. Anti-inflammatory activity of salicylic and acetylsalicylic acids and its salts /
T.V. Jakovchuk, O.V. Katjushina, D.R. Husainov, I.I. Korenjuk, T.V. Gamma, I.V. Cheretayev,
O.I. Kolotilova // Scientific Notes of Taurida V.I. Vernadsky National University. Series: Biology, chemistry.
2011. Vol. 24 (63), No 2. . 332-338.
In job with the "formalin test" are showed the presence of anti-inflammatory activity acetylsalicylates of
cobalt (ASA) and zinc (ASC) and salicylates of cobalt (SK) and zinc (SZ). In this case, ASC was more
effective anti-inflammatory agent than aspirin, that is a reference anti-inflammatory drug.
Keywords: aspirin, salicylate of cobalt, salicylate of zinc, acetylsalicylate of cobalt, acetylsalicylate of zinc,
"formalin test".

22.05.2011 .

338


. . .
, . 24 (63). 2011. 2. . 339-346.

615.281 + 547.787.1


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120
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1:10
120
17,44
2,497
6,99
0,256

1:2
120
43,83
1,851
3,81
0,299
1:2
90
38,18
1,252
3,19
0,151
1:2
60
31,72
2,439
2,91
0,137
1:2
45
26,22
1,492
2,61
0,231
1:2
30
13,64
0,903
1,65
0,121
1:2
15
3,51
1,892
0,78
0,115

2 :


, 4, .
,


.
.

343

.., .., .., ..

4

2-

150
150
,

%
%
%

%
%
,

85

45

96

37 %

67

2 ,

98

45

97

28 %

39

4
240

54

3 ,

75

90

78

4
240

81

,
2

83

130

94

4 ,
240

29

78

90

87

2 ,

62

120

85

27

55

2 ,

83

47

2 ,

87

24

2 ,

79

N
H
N
N
H
N
N

N
N
H

HO
N
N
H
O

N
N

N
H

N
N

N
N
H
N
N
H

344

, , ,
.
-
- [1].

-,
,

.
-
,
(. 5).
5

2-


140

30

56

78

72

42

69

85

24

68

83

68

78

N
N

NH2

H
N
N

NH2

H
N
NH2
N
H

NH2

N
N
H
H 2N
N
N
H

N
N

345

.., .., .., ..

1.
, .
.
2.
-
2 . .
3. 2-()
-,
.

1.
2.
3.
4.
5.
6.

.. / .., .., .. //
. 1966. .35. 2. .271-285
Grimmett M.R. Imidazole and benzimidazole synthesis / Grimmett M.R. // 1997. Academic Press -P. 63-103
Pingwah Tang. Organic Syntheses Boric acid catalyzed amide formation from carboxylic acids and
amines: N-benzyl-4-phenylbutyramide // Organic Syntheses. 2005. - Vol. 81 - P.262
.. 1,2-(2--5--2--3-) / .., .., // ,
. . 1999. 5. . 1011-1013
. . . I.
/ . ., . ., . . // . - 1962. - .
32 C. 2617 -2624
. . . II.
/ . ., . ., . . // . - 1962. . 32 . 2624-2633

.. i / .. , .. , .. , .. //
. .. . , . 2011.
. 24 (63), 2. . 339-346.
.
.
2-i .
-.
: , , i, , ,
, .
Baevsky A.M. Boric acid-s in reactions of condensation of carboxylic acids of o-phenilendiamine / A.M.
Baevsky, V.V. Tsykalov,. M.Y. Baevsky, A.B.Shelud'ko // Scientific Notes of Taurida V.Vernadsky National
University. Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 339-346.
The reaction of condensation of carboxylic acids with aromatic amines in the presence of boric acid is studied.
The high efficiency metaboric acid as condensing agent. Synthesized a wide range of derivatives of
benzimidazole and 2-aminoalkilbenzimidazoles. The structure of the synthesized compounds was confirmed
by NMR spectroscopy.
Keywords: aniline, carboxylic acid, phenylendiamin, metaboric acid, condensation, acylation,
benzimidazole.

17.05.2011 .

346

. . .
, . 24 (63). 2011. 2. . 347-351.

539.193

-- 2-
.., - ..
- , ,
E-mail: envervaliev@rambler.ru
2- 2-
CCl4 - NO2- 2272.

- 2-, - 2-
. - - 2.
: 2-, , - , - , , .


. ,
, [1].

(). ,
(-) (-),
-- (),

- -. .

- -.
-,
- ,
( ) . 2-
(2-), .
2- CCl4 75
-. [2]
2- ,
~ 200, 2-,
-.
, 2-
.
2- .

347

.., - ..

2- CCl4 UR-20.
, , ,
. 2- 0,3-0,4
/. 2,4,6-
(), 0,7 - 2,5 /.
CaF2
22 72.
10 ,
. - 0,5.

. ,
[3].

2-
,

(),
-. , ,

-, - - (
) -,
. . 1 2-
B-Y.

.1.
2- +
2- .

348

-- 2-

1 , 2-
, - .
13 2- ( ); 12 ( );
23 - 2- .
13 23, 12 .
23
23:
,

,
2- ,

2- -.

- 2-.
. 2 2- 2- + l4
13003700 1.

. 2. 2- l4 (1,3) 2- + l4 (2,4).
=0,292 (1,3); 0,321
(2) 0,395 / (4).
=2,380 (2) 0,699 / (4). l = 40 (1), 50 (2), 177 (3) 107
(4). = 22.
. 2 , 3250 1 2- ,
,
.
23
SNO2 1327 1, ,
. 2- CCl4
, , ,
OHO=P, .
1327 1 (.2) 1343
1, , , SNO2
.
, - 2-

349

.., - ..

. ,
, - 2- (. 1).
,
1327 1 ,

.

. 23, lgK23 1/T,
.
.

2-+

2-
2-

10-4
21
5,52
7,19

13
8,6
10,3

23
/
2,0
3,4

12
6,6
6,9

13 .


.
, -
,
.

,
[4] : = 5,3 ( - 0 )

l
= (cl) -1 ln 0 d (ln v ) , 0
l
- 2-,
2-. 0 - 2-,
, 0 3- 4--, [5].
0 13.
,
0 3-- 4--.
2-
0,1 /. , 2- . 13 .
12.
, 12 2-,
.

350

-- 2-

[6] - 2-
8 1 /.

,
,
.

, - 2- 6,66,9 /.

1.
2.
3.
4.
5.
6.

.. / .. . .:, 1975. 432 .


..

: . . -. / .. . ., 1982.
. - / . . .: , 1982. 328.
..
/ .. // . .: , 1981. . 112155.
.. / .. , ..
// . . 1974. . 64. . 197202.
..
- / .. , .. // . 1973. . 47. 4.
. 785790.

.. - - 2- / .. , .. - //
. .. . ,
. 2011. . 24 (63), 2. . 347-351.
2- CCl4
OH- NO2- 22-72.
' - 2, - 2-
'. - - 2-.
: 2-, , - , - , , .
Valiev E.V. Estimation of cis- trans- transition energy in 2-nitrophenol / E.V. Valiev, M.I. Sheikh-Zade //
Scientific Notes of Taurida V.Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63),
No. 2. . 347-351.
IR spectra of 2-nitrophenol and and H-complexes of 2-nitrophenol with proton acceptor in CCl4 solutions in
the region of valence vibrations of OH- and NO2- groups in the temperature range 22-72C have been
received. The changes of enthalpy under the formation of H-complexes with intermolecular hydrogen bond in
the trans- form of 2-nitrophenol, the changes of enthalpy in the transforming from the cis- forms of 2nitrophenol to the H-complex with intermolecular hydrogen bond have been received. The estimation of cistrans- transition energy in 2-nitrophenol has been made.
Keywords: 2-nitrophenol, IR spectra, cis- form, trans- form, enthalpy, energy.

10.06.2011 .

351

. . .
, . 24 (63). 2011. 2. . 352-359.

: 547.792 +546.47+ 579.01

5-(2'-)-2-(2'-)-7,8--6,5--1,3,6
:
..1, ..1, ..2, ..2
1

. .. , ,
. .. ,
,
E-mail: galex0330@rambler.ru
2

5-(2'-)-2-(2'-)-7,8--6,5--1,3,6--,
.
- -. .
: -1,3,6-, , , ,
.


.
1,2,4- ,
[16].
.
1,2,4 [7] [8, 9].

2-(5-(2-)-1,2,4--3-)

.

96%-
.
... 2-(5-(2-)-1,2,4--3-) (1)
[10]. -
4000400 -1 - Nicolet Nexus 470
KBr. -
Bruker VXR-400 (400 ), Me4Si,
-d6. Candida

352

5-(2'-)-2-(2'-)-7,8--6,5--1,3,6-

albicans 885 (). - 5105


. 37 48 .
,

, .

5-(2'-)-2-(2'-)-7,8--6,5--1,3,6 ( 2,-e).
3-(-2-)-5-(2-)-1-1,2,4- (4 )
20 96%- .
512 (4,2 ) .

. ,
, , ,
.
5-(2'-)-2-(2'-)-7,8--6,5--1,3,6- ( 2,): 78 %; ... 237 0 (EtOH); (%): C
70,22; H 4,28. C20H15N5O (%): C 70,38; H 4,40. 1, , .
.: 10,1 , () (), 8,65 , (1), 8,12 , (1), 7,90 -, (1), 7,80
(1), 7,40 , (2), 7,20 , (2 + (2H), 6,95 , (2 + NH),
6,85 , (1), 6,80 , (1). -, , -1: 3380, 3270, 3056, 1629, 1292,
750.
5-(2'-)-2-(2'-)-7,8-(3-)-6,5--1,3,6 ( 2,b). 69 %; .. 209 0 (EtOH).
(%): C 71,09; H 4,66. C21H17N5O (%): C 70,98; H 4,79. 1,
, . .: 10,03 , (), 8,61 , (1), 8,08 , (1), 7,90 -, (1), 7,64
(1), 7,43 -, (1), 7,25-7,15 , ( + (2H), 7,12-6,95 , ( +
NH), 6,91 , (1), 6,86 , (1), 6,81 , (1), 6,73 , (1), 2,27 (3).
-, , -1: 3386, 1630, 1516, 1350, 1290, 750.
5-(2'-)-2-(2'-)-7,8-(3-)-6,5--1,3,6 ( 2,c). 74 %; .. 241 0 (EtOH).
(%): C 67,02; H 3,79. C20H14FN5O (%): C 66,85; H 3,90.
1
, , . .: 10,05 , () (), 8,61 , (1), 8,07 , (1), 7,89 -, (1),
7,54 -, (1), 7,42 , (1), 7,34 (1), 7,20-7,05 , (2 + (2H), 6,95-6,82 , (2 + NH), 6,75 , (1). -, , -1: 3280, 1638,
1522, 1356, 1284, 746.
5-(2'-)-2-(2'-)-7,8-(3-)-6,5--1,3,6 ( 2,d). 84 %; ... 235 0 (EtOH).
(%): C 63,72; H 3,68. C20H14ClN5O (%): C 63,91; H 3,73.
1
, , . .: 10,02 () (), 8,60 , (1), 8,08 , (1), 7,89 -, (1),
7,72 (1), 7,59 (1), 7,42 , (1), 7,28 , (1), 7,25-7,15
, ( + (2H)), 6,96 (NH), 6,87 , (2), 6,75 , (1). -, ,
-1: 3266, 1626, 1522, 1354, 1282, 752.

353

.., .., .., ..

5-(2'-)-2-(2'-)-7,8-(3-)-6,5--1,3,6 ( 2,e). 79 %; ... 237 0 (EtOH).


(%): C 57,25; H 3,24, C20H14BrN5O (%): C 57,14; H 3,33.
1
, , . .: 10,05 , () (), 8,60 , (1), 8,07 , (1), 7,90-7,82 -,
(2), 7,61 (1), 7,40-7,30 , (2), 7,20-7,10 , ( + (2H)),
6,97 (NH), 6,87 , (1), 6,82 , (1), 6,75 , (1). -, , -1:
3268, 1626, 1520, 1354, 1284, 752.

2 2 10 96%-
438 (2 ) .

. ,
, .
[Zn2L2]0,5EtOH (H2L - 3-(-2-)-5-(2-)-11,2,4-) ( 3,). , %: Zn 15,62; N 16,36. C41H29N10O2,.5Zn2
, %: Zn 15,59; N 16,66. -, , -1: 1610 - n(=N), 1593, 1533,
1457, 1444, 1330, 1147, 752
[Zn2L21]2EtOH (2L1 - 3-(-2-)-5-(2--4-)-1-1,2,4-) ( 3,b). , %: Zn 14,22; N 15,14.
C46H42N10O4Zn2 , %: Zn 14,10; N 15,07. -, , -1: 1608 n(=N), 1534, 1462, 1444, 1378, 1324, 1146, 754.
[Zn2L22]EtOH (2L2 - 3-(-2-)-5-(2--4--)1-1,2,4-) ( 3,c). , %: Zn 14,89; N 15,74.
C42H30F2N10O3Zn2 , %: Zn 14,70; N 15,71. -, , -1: 1610 n(=N), 1534, 1462, 1444, 1324, 1170. 1150, 758.
[Zn2L23]EtOH (2L3 - 3-(-2-)-5-(2--4--)1-1,2,4-). ( 3,d). , %: Zn 14,09; N 15,26.
C42H30Cl2N10O3Zn2 , %: Zn 14,18; N 15,15. -, , -1: 1610 n(=N), 1532, 1448, 1368, 1326, 1172, 1148, 756.
[Zn2L24]EtOH (2L4 - 3-(-2-)-5-(2--4--)1-1,2,4-. ( 3,e). , %: Zn 13,15; N 14,01; 49,79; 2,88.
C42H30Br2N10O3Zn2 , %: Zn 12,93; N 13,82; 49,75; 2,96. -,
, -1: 1610 - n(=N), 1532, 1446, 1366, 1326, 1174, 1148, 756.


2-(5-(2-)-1,2,4--3-)
( 2,-e) 96%-
( 3,-e) ():

354

5-(2'-)-2-(2'-)-7,8--6,5--1,3,6-
H

R
N

N
N

OH

N N

NH2

2
2
2
2
2

R - H, CH3 , F, Cl, Br

R-H,
b R-CH3,
c R-F,
d R-Cl,
e R-Br

N N

N
N

N N
H

HO

HO
B

Zn(CH3COO)2
R
N
N

3 R-H,
3 b R-CH 3,
3 c R-F,
H
3 d R-Cl,
3 e R-Br

N N

O Zn Zn
N

N N

N
R

2-(5-(2-)-1,2,4-3-) :
, () , ().
R

N
N

N N
H

N
N

N N

HO

NH
OH

2,-e - .
-
.
2,-e

.
,
- -. 3,
[11].
2,-e 3,-e
Candida albicans 885.

355

.., .., .., ..

. 1. ,
2,-c, 3, 3,c.
() 500 /.

. ,
, 37 0
48 , , 2,-c.
, ,
500 /. , 3, 3,c,
. , ,
500 /.
1.
2,- 3,-
Candida albicans

(/)
500
250
125

*
2,
3,
2, b
3, b
2, c
3, c
2, d
3, d
2, e
3, e
* -
, .

( 30 /)
.
. 2.
, . 2,
. ,
2,-e
500 / 2,5 22,7 /.
228-250 /
11,4 / 2,b-e. 2,c
250 /
6,3 /. ,

356

5-(2'-)-2-(2'-)-7,8--6,5--1,3,6-

2
2,-e 3,-e
Candida albicans

2,
3,

2,b
3,b

2,c

3,c
2,d
3,d

2,e
3,e


(/)

48

24
48
72
96
455
22,7
-
500
12,5
455
22,7
228
11,4
114
5,7
+
+
+
+
500
12,5
455
22,7
228
11,4
+
+
+
500
12,5
+
+
+
455
22,7
500
12,5
+
+
+
+
500
6,25
+
+
+
+
500
2,5
+
+
+
+
455
22,7
228
11,4
+
+
+
500
12,5
250
6,3
+
+
+
455
22,7
228
11,4
+
+
+
+
500
12,5
+
+
+
455
22,7
228
11,4
+
+
+
500
12,5
455
22,7
228
11,4
+
+
+
500
12,5
+
+
+
500
6,25
+
+
+
455
22,7
228
11,4
+
+
+
500
12,5
455
22,7
228
11,4
+
+
+
500
12,5
+
+
+
+
500
6,25
+
+
+
+
500
2,5
+
+
+
+

357

.., .., .., ..

455-500 /
22,7 /.
. ,
3,
228 / 11,4 /. ,
,
.
.

,



. ,
.

Candida albicans.

1.

Kane J.M. ChemInform Abstract: 2,4-Dihydro-3H-1,2,4-triazol-3-ones as Anticonvulsant Agents / J.M.


Kane, B.M.Baron, M.W. Dudley [et al.] // J. Med. Chem. 1990. Vol. 33, 10. P. 27722777.
2. Chu Yong. Synthesis of Novel Antifungal Triazole Compounds / Yong Chu, Ming Xia Xui, Ding Lu //
Chin. Chem.Lett. 2004. Vol. 15, 9. P. 10111014.
3. Nowaczyk A. Triazole derivatives with antifungal activit: a pharmacophore model model study / A.
Nowaczyk, B. Modzelewska Banachiewicz // Acta Poloniae Pharmaceutica Drug Research. 2008.
Vol. 65, . 6. P. 795798.
4. Eto H. New Antifungal 1,2,4-Triazoles with Diuoro(heteroaryl)methyl Moiety / Hiromichi Eto,
Yasushi Kaneko, Takao Sakamoto // Chem. Pharm. Bull. 2000. Vol. 48, No. 7. P. 982990
5. Kokil G. R. Synthesis and In Vitro Evaluation of Novel 1, 2, 4-Triazole Derivatives as Antifungal
Agents / Ganesh R. Kokil, Prarthana V. Rewatkar, Sandeep Gosain [et al.] // Letters in Drug Design &
Discover. 2010. Vol. 7. P. 4649.
6. Eto H. New Antifungal 1,2,4-Triazoles with Diuoro(substituted sulfonyl)methyl Moiety / Hiromichi
Eto, Yasushi Kaneko, Sunao Takeda [et al.] // Chem. Pharm. Bull. 2001. Vol. 49, 2. P. 173182.
7. Bagihalli G.B. New Antifungal 1,2,4-Triazoles with Diuoro(substituted sulfonyl)methyl Moiety / G.B.
Bagihalli, S.A. Patil, P.S. Badami // Iran. Chem. Soc. 2009. Vol. 6, 2. P. 259270
8. Badwaik V.B. Synthesis, Characterization, and Biological Studies of Some Schiff Base Complexes. /
V.B. Badwaik, A.S. Aswar // Russ. Journal of Coord. Chem. 2007. Vol. 33, 10. . 755760.
9. Yadawe M.S. Synthesis, characterization and biological studies of cobalt(II) and nickel(II) complexes
with new Schff bases / Mallikarjun S.Yadawe, Sangamesh A. Patil // Trans. Met. Chem. 1997. Vol.
22. P. 220224.
10. .. 3-(-2-)-5-(2)-1-1,2,4- .., . ., .. [ .] //
. .. .
" ". 2009. . 22(61). . 154156
11. Pat. 4.198.513 USA. 1,2,4-Triazoles. Baldwin J. et al. Assignee: Merck & Co., Inc. (Rahway, NJ).
05/894.450. Filed. April 7. 1978, Published April 15. 1980.

358

5-(2'-)-2-(2'-)-7,8--6,5--1,3,6-

..
5-(2'-)-2-(2'-)-7,8--6,5--1,3,6-
: / .. ,
.. , .. , .. //
. .. . , . 2011. . 24 (63), 2. . 352-359.
5-(2'-)-2-(2'-)-7,8--6,5--1,3,6-,
. - . .
: -1,3,6-, , , ,
.
Gusev A.N. 5-(2'-Hydroxyphenyl)-2-(2'-pyridyl)-7,8-benzo-6,5-dihydro-1,3,6-triazaindolizines and
coordination compounds based on it: synthesis and antifungal activity / A.N. Gusev, V.F. Shulgin,
O.N. Postnikova, Y.L. Krivorutchenko // Scientific Notes of Taurida V.Vernadsky National University.
Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 352-359.
5-(2'-Hydroxyphenyl)-2-(2'-pyridyle)-7,8-benzo-6,5-dihydro-1,3,6-triazaindolizines and zinc coordination
compounds based on it were obtained. The structure was determined by IR and NMR spectroscopy methods.
Antifungal activity was investigated.
Keywords: dihydro-1,3,6-triazaindolizines, coordination compounds, zinc, miramistin, antifungal activity.

22.05.2011 .

359

. . .
, . 24 (63). 2011. 2. . 360-366.

547.793.4



.., ..
, ,
E-mail: 1977andr13@mail.ru
.

1,3,4-.
, .
-.
: , 1,3,4-, ,
.



[1, 2].

.

,
.

13 .
, 1 ,
[3].
1
+
+

C2H5OK

- C2H 5OH
O

H2 N

OC2 H5

SK

S
C

+ C2 H5 OK
H3C

NH
H2 N

H3C

+ C2H5OK
O

NH2

+ H3C

- C2H5OH

O
OC 2H5

- C2H5OH
O

CH3

NH

H3 C

360

SK

H3C

SK

2--4,6- 2
[4],
[5] 3
( 1). 13
. 1
4ak. 5ak ( 2).
,
[6].
2
O

NH +
H 2N

Cl

SK

(a),

H3COOC
O
S NH
O

NH

KCl

H2N

4a -k

1
R=

NH

COOCH3 (b),
H5C2OOC

() ,

NH

5a -k

O
S NH2 (d) ,
O

(c),
CH3

N
(f) ,

COOC2H5 (k).

23
.
89,
1213,
. ,
23 ,
67. ( ,
, )
1,2,3- 89 ( 3).
3
1) SOCl2

+ Cl

SK

2 -3

OH

- KCl

6 -7

OH

2) H N
2

R=

H3C

N N
R

- SO2
- HCl

N N

+R X

SK - KCl

O
8- 9

H3C

(a),

SR

10 -11 a - c

NH NH2

( 3 , 7 , 9, 11, 13 ). R = CH3 (a),

OMe

(b),

(c).

OC 2H 5

O
NH

( 2, 6 , 8 , 10 ,12 ) ,

O
O

NH2

3) CS2 , KOH

CH3

12 -13 a - c
R =

+R X

O
R

(b),

NH

(c) . X = Cl, Br.

OMe

.
1011. 10
,
(Br2, I2, SeBr4, PhSeBr3) .

361

.., ..



().
. 1 Varian
VXR-300 (300 ) MCO-d6 .
5a, 5d 5k [6].
4--2--1,6--6- 3. 0,60
30 . ,
0,50 0,50
. 2 .
30 . 3
0,50 .
3 ,
. 1 (DMSO-d6): 2,07 (3H, CH3); 5,68 (1H, CH); 12,27 . c (1H,
NH). 89 %. (%): N 19,66. 5H5N2OSK. (%): N 19,70.
5ak 1213a.
, 0,50 1 0,50 4b
,
25 .
(6080 ) 1 .
5ak 1213a , ,
.
(4--6--1,6--2-)
5b. 1 (DMSO-d6): 3,85 (3H, CH3); 4,39 (2H, SCH2); 4,96 c
(1H, CH); 6,60 (2H, NH2); 7,61 (2H, 2CH); 7,91 (2H, 2CH); 11,58 (1H, N).
85 %. (%): N 16,72. 14H14N4O4S. (%): N 16,76.
N1--2-(4--6--1,6--2-)
5. 1 (DMSO-d6): 1,18 (4H, 2CH2); 1,68 (4H, 2CH2); 3,49 (2H, CH2);
3,71 (2H, CH2); 4,97 (1H, NH); 6,52 c (2H, NH2); 7,94 (1H, CH); 11,65 (1H, NH).
71 %. (%): N 19,80. 12H18N4O2S. (%): N 19,84.
N1-[4-(1,3--2-)]-2-(4--6--1,6--2) 5. 1 (DMSO-d6): 4,00 (2H, NH2); 5,04 c (1H,
NH); 6,54 (2H, CH2); 6,83 (1H, H); 7,26 (1H, H); 7,76 (4H, 4CHPh); 10,50 c (1H,
NH). 68%. (%): N 19,06. 15H14N6O4S3. (%): N 19,17.
(4--6--1,6--2-)-4,5,6,7-[b]
-3- 5f. 1 (DMSO-d6): 1,27 (3H, OC2H5); 1,72
(4H, 2H2); 2,60 (4H, 2CH2); 4,08 (2H, NH2); 4,28 (2H, OC2H5); 5,07 (1H,
NH); 6,51 (1H, H); 11,61 (1H, NH). 61 %. (%): N 13,68.
17H20N4O4S2. (%): N 13,72.
(4,6--2-) 12.
1 (DMSO-d6): 1,31 (3H, OC2H5); 2,32 c (6, 2CH3); 4,07 (2H, SCH2); 4,30
(2H, OC2H5); 6,96 (1H, HPh); 7,73 (2H, 2Ph); 7,92 (2H, 2H); 10,58 (1,
NH). 79%. (%): N 12,08. 17H19N3O3S. (%): N 12,17.

362

(4,6--2-) 12b. 1
(DMSO-d6): 2,35 2c (6H, 2CH3); 3,79 c (2, SCH2); 4,34 (2H, NH2); 6,97 (1H, ); 9,23
(1H, NH). 73%. (%): N 26,37. 8H12N4OS. (%): N 26,39.
(4--6--1,6--2-)
13b. 1 (DMSO-d6): 2,65 c (3, CH3); 3,37 (2H, H2); 4,74 (1, NH);
5,39 (1H, H); 9,12 (2H, N2); 14,20 (1H, NH). 71%. (%): N
12,05. 16H17N3O4S. (%): N 12,10.
[3-(4,6--2-)]
12.
1 (DMSO-d6): 1,30 (3H, CH3); 2,06 (2, CH2); 2,55 (3H, H3); 3,71
(2, CH2); 4,29 (2H, H2); 6,52 (1, ); 7,77 (2H, 2Ph); 7,91 (2H, 2Ph); 10,41
(1H, NH). 71%. (%): N 11,64. 18H21N3O3S. (%): N 11,69.
(4--6--1,6--2-)
13. 1 (DMSO-d6): 1,31 (3H, C2H5); 2,11 c (3, CH3); 4,11 c
(2H, H2); 4,30 (2, CH2); 6,01 (1H, H); 7,72 (2H, 2Ph); 7,93 (2H, 2Ph);
10,61 (1H, NH); 12,56 (1H, NH). 71%. (%): N 12,05.
16H17N3O4S. (%): N 12,10.
2-(4,6--2-) 6. 0,50
2 0,50 20
60 . 4 ,
, . 1
(DMSO-d6): 2,34 (6H, 2CH3); 3,91 (2H, SCH2); 6,97 c (1H, CH); 12,60 (1H, ).
85%. (%): N 21,00. 7H9N3O2S. (%): N 21,09.
5-(4,6--2-)-2,3--1,3,4--2 8. 0,50 6 1,0
. 6 .
0,60 2 .
,
. 1,3,4 ,
. ,
8 .
, , . 1
(DMSO-d6): 2,35 (6H, 2CH3); 4,51 (2H, H2); 7,03 (1H, NH). 80%.
(%): N 27,38. 8H9N5OS2. (%): N 27,43.
1,3,4- 89.
0,50 1,3,4- 89 20 0,50
. 0,60
. 40
. , ,
.
[5-(4,6--2-)-1,3,4-] 10b.
1 (DMSO-d6): 2,34 (6H, 2CH3); 3,65 (3, CH3); 4,19 (2H, SCH2);
4,63 (2H, S2); 7,02 c (1H, NH). 77%. (%): N 21,33.
11H13N5O3S2. (%): N 21,39.

363

.., ..

4--2-(5--1,3,4-o-2-)-1,6--6-
11. 1 (DMSO-d6): 2,51 (2H, CH2); 2,56 c (3, CH3); 2,66 (2H,
SCH2); 5,52 (1H, ); 5,93 c (1, NH). 75%. (%): N 20,69.
9H10N4O2S2. (%): N 20,73.
4--2-(5--1,3,4--2-)-1,6--6-
11. 1 (DMSO-d6): 2,26 (3H, CH3); 2,65 (2, CH2); 3,86 (2H, SCH2);
5,15 (2H, =2); 5,37 (2H, H2); 5,53 (1H, H); 6,01 (1H, =H2-); 12,98 (1,
NH). 77%. (%): N 18,85. 11H12N4O2S2. (%): N 18,90.

10b
11a

13b

0,7681
0,7292
0,7471
0,7192

0,6911
0,7652
0,8441
0,9342

0,8351
0,5722

0,8581

0,8261 0,6422
0,8122

0,8071
0,5152
0,5962
0,5192

0,7481
0,7592

0,8811

12b

( )

5ak,
69, 1013ac,
. , 5ak 1213
(NH) 10,5012,50 .. 10,4012,55 ..
6 1
12,60 ..
89 ,
2,35
.. 11 CH3-
2,56 .., 11
3,86 .. (SH2), 5,15 5,37 .. (=2)
6,01 .. (=-).
1

6, 10b, 11a, 12b, 13b

0,8041
0,7201

0,7001
0,6642
0,7361
0,7002

0,7451
0,7472

0,8231
0,8202
0,6471
0,6462

- PASS.
- Pharmaexpert.

364

,
.
PASS [7] Pharmaexpert [8],
500

-. 1
(Pa) .
5ak, 8, 11, 12, 12, 13
. 6,
10b, 11a, 12b, 13b [9]
[10].
[10, 11].

, 3

198,2
326,4
270,3
212,3
214,2

4
7
6
5
6

1
0
1
3
4

3
7
4
3
3

170,7
267,9
214,7
186,5
178,0

LD50 (mg/kg)
Class 4


(NH)

13
21
17
14
14

LD50 (mg/kg)
Class 4


(O,N)

63,08
91,01
84,68
80,91
100,80


c [10]

LD50 (mg/kg)
Class 5

1,394
1,831
1,194
0,194
0,969

6
10b
11
12b
13b


, 2

Log P

2
6, 10b, 11a, 12b, 13b

466,10
351,40
490,80
418,00
390,00

912,00
890,40
700,40
600,20
441,40

964,90
1778,00
884,10
634,40
911,80

1. 5ak 1213.
2. 23
1,3,4- 89.
1011.
3.
PASS, Pharmaexpert,
( 4, 5)
.
4. - (6, 12b, 13b) .

365

.., ..


1.

Studies on -[6-(2'-methoxynaphthalenyl)-4-phenylpyrimidinylthio]--(4-phenylbenzoyl)propionic acid and


the biological activity of some products / M.M. Arief, S.A. Essawy, A.A. Wasfy [et al.] // Phosphorus,
Sulfur, Silicon and the Related Elements. 1994. Vol. 91, 1,4. P. 17.
2. Synthesis and Biological Study of Some Novel 4-[5-(4,6-Disubstituted-2-thiomethylpyrimidyl)-4'-amino1,2,4-triazol-3'-yl]thioacetyl-3-arylsydnones / B. Kalluraya, B. Lingappa, S.R. Nooji [et al.] //
Phosphorus, Sulfur, Silicon and the Related Elements. 2007. Vol. 182, . 6. P. 13931401.
3. Structure-Based Design, Synthesis, and in vitro evaluation of Bisubstrate Inhibitors for Catechol OMethyltransferase / B. Masjost, P. Ballmer, E. Borroni [et al.] // Chemistry A European Journal. 2000.
Vol. 6, 6. P. 971982.
4. Pat. US 3932375 (A). Use of 2-pyrimidine thiol carbonates as acylating agents for amino or imino
containing compounds / N. Takeshi, K. Katumasa, K. Kouichi US1974047918819740613; publ.
13.01.1976.
5. Ringspaltungsreaktion von Thiadiazolo- und Triazolopyrimidin-Derivaten / T. Okabe, E. Taniguchi, K.
Maekawa [et al.] // Bulletin of the Chemical Society of Japan. 1974. Vol. 47. P. 28132817.
6. .. 4--2--6- / .. , .. , ..
// . . 2009. . 22. . 123126.
7. Orechovich V.N. Institute of Biomedical Chemistrry. PASS: http://195.178.207.233/PASS/.
8. Pharmaexpress: http://pharmaexpert.ru/PASSonline/predict.php.
9. Experimental and computational approaches to estimate solubility and permeability in drug discovery and
development settings / C. A. Lipinski, F. Lombardo, B. W. Dominy, P. J. Feeney [et al.] // Adv. Drug.
Delivery Rev. 1997. Vol. 23. P. 425.
10. Pharmaexpress, Gusar online: http://www.pharmaexpert.ru/GUSAR/acutoxpredict.html.
11. Molinspiration Cheminformatics: http://www.molinspiration.com/cgi-bin/properties.

.. /
.. , .. //
. .. . , . 2011. . 24 (63), 2. . 360-366.
.
1,3,4-.
,
. -.
: , 1,3,4-, ,
.
Kryvov'yaz A.O. Synthesis of derivatives pyrimidine and prediction their biological activity /
A.A. Kryvov'yaz, V.G. Lendel // Scientific Notes of Taurida V.Vernadsky National University. Series: Biology,
chemistry. 2011. Vol. 24 (63), No. 2. . 360-366.
On the basis of the method obtained amide derivatives of pyrimidine. Carried out further functionalization
received initial pyrimidine systems to the synthesis of 1,3,4-oxadiazole derivatives. All final products
subjected to computer prediction of biological activity spectra and checked for criterion Lipinski. Identified
compounds leaders.
Keywords: acetamide derivatives of pyrimidine, 1,3,4-oxadiazole, biological screening, criterion Lipinski.

25.05.2011 .

366

. . .
, . 24 (63). 2011. 2. . 367-374.

543.257:681.142.2

..
. .. , ,
E-mail: vkropotov@crimea.edu

: ,
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.

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.

367

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A11s U21 + A12s U22 + K + A1ms U2 m = s U 1 ,


2

A21s U21 + A22s U22 + K + A2 ms U2m


Am1s + Am 2s
2
U1

2
U2

U2

M
2
+ K + Amms U2m = s U m ,

( )

(1)

2
- Ui (i = 1, 2,,m, m - );
s Ui

(s )

Ui

Ui. Aij

.
[3]
s U2i = (k EUi s E ) 2 + (kVU i s V ) 2 ,
(2)
s E2 s V2 - ,
( pX)
(, ,
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( pX)
, .
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.
,
,
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.


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1:1
-.

368


(sV=0,001 ). ,

pM
pM (spMe=0,001 . pM).
.
:
1. (5:1:5) V= 0,30, 0,90, 1,50, 2,10, 2,40, 2,50, 2,60, 3,10, 3,70, 4,30, 4,90 ;
2. (10:1:10) V=0,30, 0,60, 0,90, 1,20, 1,50, 1,80, 2,10, 2,30, 2,40, 2,45, 2,50, 2,55,
2,60, 2,80, 3,10, 3,40, 3,70, 4,00, 4,30, 4,60, 4,90 ;
3. (19:1:20) V= 0,15, 0,30, 0,45, 0,60, 0,75, 0,90, 1,05, 1,20 1,35, 1,50, 1,65, 1,80,
1,95, 2,10, 2,20, 2,30, 2,35, 2,40, 2,45, 2,50, 2,55, 2,60, 2,65, 2,70, 2,80,
2,85, 2,95, 3,10, 3,25, 3,40, 3,55, 3,70, 3,85, 4,00, 4,15, 4,30, 4,45, 4,60,
4,75, 4,90 .
5104 5103 .

50 2,5 .

c (V - V )

(V<Ve)
pM = L1 = a1 + b1 lg t e
+ x1
V0 + V

c (V - Ve )

pM = L2 = a 2 + b2 lg t
+ x 2 , (VVe),
V0 + V

a1=pM; a2=pM+b2pK; pM pM, [M]=1 M; bi


( pM- b1=b2=1); V0
; Ve ,
; ct - ; xi -
.
p :

p =

a 2 - a1
.
b1


[4].

:
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2. ;
3. (1);
4. ( (2)).
,

369

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, 1,5 sE sV.
.
p :

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s a21 + s a22 - 2ra1 ,a2 s a1s a2


,
b12

ra1,a2 a1 a2

1-3.
,
(sV=0,001 , spM=0,001 . pM).
1 F
.

1
F
(sV=0,001 , spM=0,001 . pM, cM=510-4 M)

5:1:5
10:1:10
19:1:20
5:1:5
10:1:10
19:1:20
5:1:5
10:1:10
19:1:20
5:1:5
10:1:10
19:1:20

lg
9,4
(4,7)
3,4
(2,9)
2,4
(2,4)
2,3
(2,9)
1,5
(1,7)
2,1
(2,1)
1,1
(1,0)
(1,0)
1,2
(1,0)
1,0
(1,0)
1,0
(1,0)
1,0
(1,0)

p=8

p=10
Ve
lg
Ve

9,2
11
11
(4,7)
3,4
2,7
2,7
(2,9)
2,8
2,1
2,1
(2,3)

2,0
2,6
1,8
(1,6)
2,1
3,1
1,4
(1,2)
3,5
1,3
1,3
(1,9)

1,2
1,0
1,0
(1,0)
1,0
1,0
(1,0)
1,2
1,0
1,0
(1,0)

1,0
1,0
1,0
(1,0)
1,0
1,0
1,0
(1,0)
1,0
1,0
1,0
(1,0)

p=16
lg
Ve
7,4

7,4

2,8

2,8

2,3

2,3

2,4

4,1

1,9

3,0

1,9

1,3

1,0

1,0

1,0

1,0

1,0

1,0

1,7

22

2,0

14

3,1

32

) 20 , ) 4 20 .

370

1 , ,
, ,
- (1) .
p=8 (
) p=16 ( )
F .

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", .
, .
(1) ,
,
. p=8 cM=510-4 M
(1) ( ). -
,
( 10:1:10) (1), ,
. 510-3
M (1).

1 .

, p = 16 Ui=f(E,V)
,
.

.
2. ,
(1) (2) ( ,
).
2
B ( )
(sV=0,001 , spM=0,001 . pM, cM=510-4 M)

5:1:5
10:1:10
19:1:20
5:1:5
10:1:10
19:1:20

p=8
p=10
p=16
lg
Ve
lg
Ve
lg
Ve

14,2
14,2
16,8
16,8
16,8
16,8
17,2
17,1
18,1
18,1
16,0
16,0
21,4
21,4
13,6
13,6
16,6
16,6

3,23
3,15
4,03
1,74
3,81
12,1
0,73
3,01
2,73
0,66
2,28
6,41
2,48
0,45
0,40
2,39
0,43
8,39

371

c20.95(19)=30,1

c20.95(3)=7.81

..

2, ,
, ,
.
,
, ,
/ p=8. ,

.
- , -
[5].


, . ()
.
,
, 3. F
,
: s - , r -
, s' - (1) s" -
( (2)). 3 10:1:10,
.
3
,
, F
( 10:1:10, sV=0,001 , spM=0,001 . pM, cM=510-4 M)

8
10
12
16
8
10
12
16

s104

r104

7,04
(5,7)
5,67
5,34
6,07

7,76
(5,86)
6,22
5,67
5,47

24,7
(4,4)
5,17
4,74
3,54

27,2
(4,7)
5,00
4,20
4,04

s'104

s"104


7,2
(5,7)
(5,7)
5,65
5,7
5,63
5,7
5,63
7,02
Ve
25,3
(4,4)
(4,6)
4,38
4,6
4,07
4,2
4,06
8,26

1,22
(1,06)
1,21
1,13
1,23

F0.95(360,)=1,13
F0.95(,76)=1,34
F0.95(76,360)=1,32

1,21
(1,13)
1,39
1,34
1,32

F0.95(360,76)=1,37
(F0.95(360,)=1,13)
F0.95(76,)=1,28
F0.95(360,76)=1,37
F0.95(,76)=1,34

F0.95(360,76)=1,37

.
, F, ,
.

372

3 , , ,
p, ,
.
p=8-10. F (
10%) .
, p=16
, ,
( 3 ).
, ,
,
,
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( pX)

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.

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373

..


1.
2.
3.
4.
5.

. / . .: , 1979. - 349 .

..

: / .. // .
, . - 2006. - . 19 (58), 2. - . 104110.
Metes L. Factors affecting the precision of a new method for determining the reduced and oxidized forms
of a redox couple by a single potentiometric titration / L. Metes, N. Fanelli // Anal. Chim. Acta. - 1987.
Vol. 194. - P. 151162.
..
/ .. // . . . - 1998. - .53. 6. . 566570.
.. . / .. , .. - .:
, 1968. - 476 .

..
/ .. //
. .. . , . 2011. . 24 (63), 2. . 367-374.

: , ,
" i". ,
, .
, "
i".
: i , i, .
Kropotov V.A. Comparative characteristics of some methods for the reproducibility estimation of
sedimetric titration curve parameters / V.A. Kropotov // Scientific Notes of Taurida V.Vernadsky National
University. Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 367-374.
The paper deals with the comparison of four methods of reproducibility estimation of sedimetric titration
curve parameters (the repeated titration method, the sum of squares of deviations and two methods according
to "the law of distribution of errors"). It is established that all methods as a whole give reproducibility
estimations of a titration curve parameters correlating well in the majority of titrations. At unique titration
generally the methods based on "the law of distribution of errors" have advantage.
Keywords: potentiometric titration, error prediction, sedimetric titration.

21.05.2011 .

374

. . .
, . 24 (63). 2011. 2. . 375-384.

504.064:66.066.4:544.7:628.3



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E-mail: nat_kul_mik@mail.ru
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Na3PO4; Na2SO4; NaNO3), . . .
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d=0,34 . (0,051,5 /)
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1.
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10,
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0,05
0,10
0,20
0,25
0,30
0,40
0,50
0,60
0,70
0,75
0,80
1,00
1,20
1,25
1,50

=0
8,6
6,8
4,2
3,8
3,7
3,2
3,1
3
2,9
3
3,2
3,6
4,1
4,2
4,8

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4,7
4,2
3,7
3,5
3,2
2,7
2,7
2,7
2,7
2,8
2,8
3,2
3,7
3,8
4,3

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1,6
1,5
1,2
1,1
1
1,3
1,4
1,5
1,6
1,7
1,7
2
2,5
2,6
2,8

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0,9
0,7
0,7
0,8
0,8
0,8
0,8
0,8
0,8
0,8
0,8
0,8
0,9
1
1,3

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0,3
0,3
0,3
0,3
0,4
0,3
0,4
0,4
0,4
0,4
0,4
0,4
0,4
0,4


' .
'
, .
' , .

. -402
, ,
.

-402 (110-7/3) -
, , .
5 (.1),
.
,
.
20 60.

378

1
0,8
0,6
0,4
0,2
0
0

. 1. -402 (110-7/3) -
(=350).
-402 (110-7/3)
,
( ), . 2.
, ,
NO3 Cl PO43 SO42 3 4
[6].

1
0,8

0,6

0,4
0,2

0
3

. 2. -402 (1)
2 PO43; 3 SO42;4 NO3; 5 Cl; -402=110-7/3; ()=0,1/3

379

..

, ,
, .
-402 0,1 NaOH 6 10,
.
, Cl.
,
, , .
, ,
, .

,
.
-402 (3 25)
,
, ,
, , ,
Cl. ,
0,1 HClO4 : -402, . 3.
- ,
- .

pCl
2,6
2,5
2,4

2,3

2,2

2,1

5
0

0,5

1,5

V(HlO4),

. 3 20 110-6 -402
(.1) (5 .2; 10 .4) (5 .3; 10
.5) 0,1 HClO4.
. 3,

380

,
,
.
, ,
.
,
. , Cl
Cl4, Cl (. 3).

,
,
. -402
,
1:1.
'
, , ,
( , , ),
', .

.

-402 .
, (Cl, NO3, SO42, PO43, Na+, NH4+, Mg2+, Ca2+, Al3+, Fe3+)
.

(. 4).
,
-402 . ,
. 4, ,
,
, , .
, ,
, ',
.
,
,
, .
-402
.

,
. -402
MgCl2, CaCl2, AlCl3 FeCl3, ,

381

..

( () 0,03 / 3)
.

0,7
0,6
0,5
0,4
0,3

1
2
3
4

0,2
0,1
0
0

0,01

0,02

0,03

0,04

0,05

0,06

(), /3
. 4 -402
():
1 Na3PO4; 2 Na2SO4; 3 NaCl; 4 NaNO3;
C-402 = 110-8/ 3; V(25) = 3
-402
(. 5). ,
25%, , , .

.
.

. ,
, .
:
, ,

.
,
.
-
-402 . ,
, '
, .

382

0,6

0,5

0,4

0,3
0

10

20

30

40

50

V (25), %
. 5. -402
.
, ,
,
.

,
- ( ). ,

, , ,
.
-,
.
,
. , , ,
,
, .

1.
2.
3.

. . . / [ .., .., ..,


..] .: . , 2008. 422 .
..
/ ..
: . . ., 2000. .2. 59 .
.. ,
/ .. // . 2006. 1. . 4052.

383

..
4.

..
/ .. , .. , .. // . . 70,
1. 2004. . 39.
5. / .. , .. ,
.. [ .] // . 2006. . 11, . 2.
. 121129.
6. .. . / .. .: , 1966. 656 .
7. 402 . . / [ ..,
.., .. .] // . 1997. 7. . 15.
8. : . . / .. , .. ,
.. .; . .. . .: , 1990. 304 .
9. - / [. , . ,
. , . ]; . . .. . .: . , 2007.
528 .
10. .. / .. // . 7.
. 2009. . 914.
11. .. ,
/ .. // . 2000. - 495, . 6(29). . 914.

..
/ .. //
. .. . , . 2011. . 24 (63), 2. . 375-384
, ,
, -402 ,
, , , . ,
,
, .

-.
: , ,
, , .
Kulalayev N.V. Determination the properties of "organized media" during the research of the
polyelectrolytewater system / N.V. Kulalayev // Scientific Notes of Taurida V.Vernadsky National
University. Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 375-384.
By the methods of ionometry, viscosimetry, spektrofotometry the properties of "organized media", which is
formed by cationic polyelectrolyte VPK-402 in water solution and the impact of nature media, ionic strength
of solutions, type of a base electrolyte, temperature on it have been researched. It has been shown that
inorganic electrolytes, organic bipolar solvent, which does hydrophilization of the polyelectrolyte
macromolecules, exercise a significant influence on its behavior in a soluble. An effect that mentioned factors
have on a process of connecting polycation by anionic dyes with their quantifications in the "polyelectrolyte
water system has been researched.
Keywords: "organized media", in the "polyelectrolytewater system, cationic polyelectrolyte,
hydrophilization, confirmatory condition.

20.05.2011 .

384

. . .
, . 24 (63). 2011. 2. . 385-389.

547.551.54 +547.553 + 547.785.51

1--2-
.., .., ..
. .., ,
E-mail: b_m_y@mail.ru
1--2-.
-
.
N--- .
-.
: -, , , , ,
.

1-

. ,
,
. , 2-
N-
,
,
, ,
.
.

N
N
H

NH2

N
N

Br

+
N
H

KOH

+
NH2

N
H

N
H

N
+
N
H

385

.., .., ..

1 Varian VXR-300 (300 ),


- Me4Si. (d-).
N---. 157,5 (1 ) , 138 (1 ) 23, 1,2 100 2
120-130 24 .
.
N---, (200 )
,
.
N---. 1 ,
, ,
1 N--- 300 -2.
,
150 85%- .

,
. ,
.
1--2-.
1 N--- 1
300
( 48 96
). ( ) ,
-2.

1--2- , N -
[1].
-, - ,

.
N---,
(I) - (II) [2]
N--- (III)
[3]. N---
:
-
140 ;

386

1--2-

- 4N .
O
+
N

II

+ N R

O
+
N

K2CO3

Cl
I

III

O
NH2

R'

R'

N
R

IVa R'=

R=Met

IV

IVd R'=
IVe R'=

N
R=Et

IVi R'=

N
R=iPr

IVf R'=

R=iPr

IVg R'=

IVj R'=

R=Et

IVh R'=

N
R=Et

IVc R'=

N
R

R=Met

N
IVb R'=

O H2N NH2
R

R=iBu

IVk R'=

R=Et
R=iBu

R=Met


,
. 4N
( 72 ),
, .
1
1.
.
IVa IVb IVc IVd IVe IVf IVg IVh IVi IVj IVk
, %

82

76

79


2, 3

80

78

387

76

68

74

75

75

82

.., .., ..

2.
- IVa-j ( DMSO-d6).

IVa

CH3
CH2CH3
CH2CH3
CH(CH3)2
CH(CH3)2
CH2CH(CH3)2
CH2CH(CH3)2
CH2CH(CH3)2
NH2
CH2NH2
CH2CH2NH2
CH2CH2NH2
CH(NH2)CH3
CH(NH2)CH3
CH2NHCH3
CH2NHCH3
C4
C5,6
C7
1

IVb

IVc

IVd

4.05

4.03

4.61

4.57

1.42

1.42

5.00

1.63

R`
9.32 9.42 9.15 8.63 8.68
4.61 4.61c 4.60

3.42 3.46

3.65 3.69

Ar
7.91 7.97 7.99 7.95 8.01
7.53 7.56 7.43 7.58 7.58
7.83 7.85 7.79 7.80 7.82

IVg

IVh

IVi

IVj

5.22
1.69

4.53 4.55

1.40 1.39

4.35

4.24
2.19

2.16
0.96

0.90

8.69

8.62

9.29 10.21 9.97

3.40
3.75

3.45
3.67

5.09

1.74

4.74

2.75

8.10
7.55
7.84

8.00
7.56
7.81

7.88 7.91 7.83


7.49 7.50 7.41
7.80 7.82 7.77

4.67
2.75

3.
- IVk ( DMSO-d6)

H2
C 4
CH2
HC 6 C
C 2CH
CH2
C
HC
3
8
N
10 N
C9
H
H
CH3
7

IVe
IVf

H
C

1
2
3
4
5
6
7
8
9
10

. (..),

4.02
5.25
3.40
2.09, 2.21
2.54
7.45
7.79
7.79
7.45
9.81

1. -
.

388

1--2-

2. 1--2-,
.

1.
2.
3.

.. / .. , .. , .. //
. 1966. .35. 2. .271
Zhu Y-F. New Modified Preparation of 2-Nitrodiarylamines / Y-F Zhu, G-Q Lin, Y-Q.J. Chen //
Synthesis [SYNTBF], 1990 P. 430.
K.S. Sharma Condensed Heterocycles; XI. Synthesis of 1,2,5-Thia(selena)diazolo[3,4-b]quinolines and
1,2,5-Thia(selena)-diazolo[3,4-h]quinolines / K.S. Sharma, Sharda Kumari, Ram Phul Singh. //
J. Synthesis, 1981 4 P. 316-318

.I. 1--2-- / .I. ,


.. , .. //
. .. . , . 2011. . 24 (63), 2. . 385-389.
1--2-. i
-
'
ii N- .
-.
: -, , , , ,
.
Poddubov A.I. Synthesis of derivatives of 1-alkil-2-aminoalkilbenzimidazola / A.I. Poddubov,
V.V. Tsikalov, M.U. Baevsky // Scientific Notes of Taurida V.Vernadsky National University. Series:
Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 385-389.
A new scheme of synthesis of obtaining derivatives of 1-alkyl-2-aminoalkyl-benzimidazoles is develop. This
scheme is based on nucleophilic substitution of halogen in the o-benzene at nitrogalogen derivatives
aminoalkyl radical. After that, the synthesized compound regains hydrazine to the corresponding N-alkyl-ophenylenediamine and condensed by Phillips with a number of amino acids. The structure of the synthesized
compounds were confirmed by NMR spectroscopy.
Keywords: o-nitrochlorobenzene, alkylamine, carboxylic acid, condensation, alkylating, benzimidazole.

24.05.2011 .

389

. . .
, . 24 (63). 2011. 2. . 390-395.

582.573.16:581.192


ALLIUM CYRILLII (ALLIACEAE)
..1, ..2, ..3, ..4, ..4
1

, ,
, ,
3
. .. , ,
4
. .., ,
E-mail: tolkacheva_n@mail.ru
2

() Allium
cyrillii Ten. .
, 3--D--(13)-[--D--(12)]---D--(14)---D-(25R)-5--2,3,6-.
: Allium cyrillii Ten., .


,
[1].
, ,
Amaryllidaceae, Dioscoreaceae, Alliaceae, Scrophulariaceae, Solanaceae,
,
,
,
,

[24]. ,
[5, 6].
,
[7].

Allium, , ,
.

Alliaceae .

,
() Allium cyrillii Ten., 2010 .

390

170 70% .
.
50
- .
. ()
-- (65:35:7) - (7:3).
Sorbfil -, 110 .
[8] [9],
120 . : 10
110 5 1%-
2- .
.
.
- 9:1 .
.
-- 4:5:1.
100
Supelcoclean C18 (150-200 , Supelco).
Agilent 1100, (ELSD)
Zorbax SB-C18 (1502.1 ).
, 0,6 (400 1H; 100
13) Varian AS 400, .

A. yrillii 0,11
. .

, 2:1:1.
13 - (. 1) 27 ,
: (14,8; 16,3; 17,0; 17,1),
, (36,8; 40,6; 109,0)
. 1- 13 - ,
, 13
-22 109,0 .. -5 ( 47,6) -9 (54,3)
-19 (17,0) - [1].
3-27,
c 3,49 (1, -26 3.58 (1, -26b)
3-27 [1]. ,
(25R)-. , 25R-
-20, -21, -23, -24, -25 -27
[10, 11]. 5-
1,28 .. (-19)

391

.., .., .., .., ..

(..) 36,8 (-10), 54,3 (-9), 47,6 (-5) 46,9 (-1)


HMBC.
( -2 -6
) -
[1213]
- (
10 ..) , , -. 2,3,6-
-
-2, -3 -6 -.
[1215]
(25R)-5--2,3,6-
-3 ( 76,5 .. 84,4 ..)
1

-
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27


46,9
70,5
84,4
31,7
47,6
69,8
40,5
29,8
54,3
36,8
21,1
39,9
40,6
56,0
32,0
80,9
62,8
16,3
17,0
41,8
14,8
109,0
31,6
29,0
30,4
66,6
17,1

-
Gal-1'
2'
3'
4'
5'
6'


102,9
72,3
75,3
79,1
75,5
60,4

Glc-1''
2''
3''
4''
5''
6''

104,4
81,0
86,8
70,2
77,3
62,7

Glc-1'''
2'''
3'''
4'''
5'''
6'''

104,5
75,8
77,9
71,2
78,2
62,5

Xyl-1''''
2''''
3''''
4''''
5''''

104,7
74,9
78,4
70,3
67,1

392


: 0,85 (3, s, -18), 1,28 (3, s, -19), 0,69 (3, d, J = 7,0 Hz,
-27) 1,14 (3, d, J = 7,0 Hz, -21).
: 5,56 (1, d, J = 7,5 Hz), 5,24 (1, d, J = 7,5 Hz), 5,20 (1, d, J = 7,5 Hz)
4,96 (1, d, J = 7,5 Hz). -
- .
1- 13 - , -3
.

TOCSY COSY. 13C-
HSQC. ,
. 2, -D, -D- -D-.
2
HSQC

1a
1b
2
3
4a
4b
5
6
7a
7b
8
9
10
11a
11b
12a
12b
13
14
15a
15b
16
17
18
19
20
21
22
23a
23b
24a
24b

HMBC

HSQC
2,21 dd
1,25 m
4,09 m
4,01 m
2,38 q
2,12 m
1,18 m
3,96 m
2,00 m
1,17m
2,15 m
0,75 td

C-2, 3, 5, 10, 19
C-2, 9, 10, 19
C-3
C-2, 1'
C-2, 5, 10
C-5, 10
C-4, 10, 19
C-8, 10
C-5, 6, 9
C-8
C-6, 9, 13, 14
C-8, 10, 19

1,54 m
1,42 m
1,70 m
1,09 m

C-8
C-8
C-9, 13, 14
C-9, 11, 13

1,14 m
2,08 m
1,43 m
4,57 m
1,83 t
0,85 s
1,28 s
1,94 m
1,14 d

C-8, 12, 13, 15, 17, 18


C-8, 13, 14, 16, 17
C-8, 14, 16
C-13
C-12, 13, 15, 16, 18, 20, 21
C-12, 13, 14, 17
C-1, 5, 10
C-13, 17, 21, 22, 23
C-17, 20, 22

1,67 m
1,62 m
1,57 m
1,53 m

C-20, 22, 24
C-22, 24, 25
C-22, 23
C-22, 23, 25

393

25
26a
26b
27

HSQC
1,59 m
3,58 dd
3,49 t
0,69 d

1'-Gal
2'
3'
4'
5'
6'a
6'e
1''Glc
2''
3''
4''
5''
6''a
6''e
1'''Glc
2'''
3'''
4'''
5'''
6'''a
6'''e
1''''Xyl
2''''
3''''
4''''
5''''a
5''''e

4,96 d
4,54 m
4,12 m
4,59 m
4,02 m
4,59 m
4,19 m
5,20 d
4,34 t
4,14 t
3,79 t
3,85 m
4,48 m
4,05 m
5,56 d
4,03 m
4,12 m
4,03 m
3,89 m
4,53 m
4,40 dd
5,24 d
3,94 t
4,07 m
4,11 m
3,66 t
421 m

HMBC
C-24
C-22, 24, 25
C-22, 24, 25, 27
C-24, 25, 26
C-3, 3'
C-3'
C-5'
C-2', 3', 5', 1''
C-6'
C-5'
C-4', 5'
C-4'
C-3'', 1'''
C-1'''', 1'', 2'', 4''
C-3'', 5'', 6''
C-4'', 6''
C-4'', 5''
C-2'', 3'''
C-3'''
C-1''', 2''', 4''', 5'''
C-6'''
C-6'''
C-4'''
C-4''', 5'''
C-3'', 3''''
C-3'''', 4''''
C-5''''
C-3'''', 5''''
C-1'''', 3'''', 4''''
C-1'''', 3'''', 4''''

.., .., .., .., ..

-3 (84,4) -1 Gal (4,96)


. -4 Gl (79,1)
, .
HMBC -
4,96 .. (-1 Gl') 81,0 .. (2 Glc''), H-1 Xyl (5,24) C-3 Glc'' (86,8); H-1 Glc''' (5,56) C-2
Glc'' (81,0); H-1 Glc'' (5,20) C-4 Gal (79,1).
, Allium
cyrillii
Ten.

3---D--(13)-[--D-(12)]---D--(14)---D-(25R)-5--2,3,6-.
25

21
18

27

22

O
19

HO
2

Xyl(1-->3)Glc(1-->4)Gal
Glc(1-->2)

OH

1. Allium cyrillii Ten.


.
2. ,
3---D--(13)-[--D-(12)]---D--(14)---D--(25R)-5-2,3,6-.

1.
2.
3.
4.
5.

/
[.. , .. , .. .]. : , 1987. 142 .
..
Dioscorea deltoidea Wall. / .. , .. // . .
. 1995. . 31. . 238242.
Multiple functions of sterols in yeast endocytosis / A. Heese-Peck, H. Pichler, B. Zanolari [et al.] // Mol.
Biol. Cell. 2002. Vol. 13. P. 26642680.
Oleszek W. Saponins in food, feedstuffs and medicinal plants / W. Oleszek, A. Marston // Phytochemical
Society of Europe. 2000. Vol. 45. . 241254.
Steroid saponins from fenugreek and some of their biological properties / Y. Sauvaire, Y. Baissac, O.

394

6.
7.
8.
9.
10.
11.
12.
13.
14.
15.

Leconte [et al.] // Adv. in Exp. Med. and Biol. N.Y. London: Plenum Press. 1996. Vol. 405. P.
3746.
.., .., .. //
. . . 1982. 1. . 9597.
/ ..,
.., .., .. //
2009. . 45, 5. . 517526.
Kiyosawa S. Detection of Prototype Compounds of Diosgenin and Other Spirostanol-Glycosides / S.
Kiyosawa, M. Huton // Chem. Pharm. Bull. 1968. Vol. 16. P. 11621164.
Sannie M. Steroid Sapogenins 7. Neoruscogenin 3--Dihydroxy-22-25L-S-Spirostene, a new Sapogenin
from Ruscus aculeatus / M. Sannie, H. Lapin // Bull. Soc. Chem. France. 1957. Vol. 10. P. 1237
1241.
Ikeda T. Pregnane- and furostane-type oligoglycosides from the seeds of Allium tuberosum /T. Ikeda, H.
Tsumagari, M. Okawa [et al.] // Chem. Pharm. Bull. 2004. Vol. 52. 1. P. 142145.
Su L. Steroidal saponins from Tribulus terrestris /L. Su, G. Chen, S.-G. Feng [et al.] // Steroids. 2009.
Vol. 74. P. 399403.
Agrawal P.K. Carbon-13 NMR spektroskopy of steroidal sapogenins and steroidal saponins / P.K.
Agrawal, D.C. Jain, R.K. Gupta [et al.] // Phytochemistry. 1985. Vol. 24. 11. P. 24792496.
Agrawal P.K. Assigning stereodiversity of the 27-Me group of furostane-type steroidal saponins via
NMR chemical shifts / P.K. Agrawal // Steroids. 2005. Vol. 70. P. 715724.
Peng J. 19 New steroidal saponins from Allium plants: isolation, structural elucidation and effect on
blood coagulability / J. Peng, X. Yao // Saponins used in traditional and modern medicine. Plenum press.:
New York, 1996. Vol. 404. P. 511526.
Hayes P.Y. Steroidal saponins from the roots of Trillium erectum (Beth root) / P.Y. Hayes, R. Lehmann,
K. Penman [et al.] // Phytochemistry. 2009. Vol. 70. P. 105113.

.. Allium cyrillii (Alliaceae) /


.. , .. , .. , .. , .. //
. .. . , . 2011.
. 24 (63), 2. . 390-395.
() Allium cyrillii
Ten. .
, 3---D--(13)-[--D-(12)]---D--(14)---D--(25R)-5-2,3,6-.
: Allium cyrillii Ten., .
Tolkachova N.V. Structure of basic steroidal glycoside of raseme of Allium cyrillii (Alliaceae) /
N.V. Tolkachova, G.P. Zaytsev, V.V. Kachala, V.I. Grishkovets, V.Ya. Chirva // Scientific Notes of Taurida
V.Vernadsky National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 390-395.
As the result of the research of steroidal glycosides of raseme of Allium cyrillii Ten. The main spirostanol
saponin has been obtained. With the help chemical and spectral methods the structure of the given compound
has been determined. It is 3---D-xylopyranosyl-(13)-[--D-glucopyranosyl-(12)]---Dglucopyranosyl-(14)---D-galactopyranoside-(25R)-5-spirostan-2,3,6-triol.
Keywords: Allium cyrillii Ten., steroidal glycosides.

18.05.2011 .

395

. . .
, . 24 (63). 2011. 2. . 396-401.

547.455.6232:612.014.46



.., .H., .., ..
. .. , ,
E-mail: tachup@rambler.ru

15--5

2--3,4,6----2---D . - , 1
.
: , -, , .

,
.
, ,
,

.
, ,
,
,
,
. ,


.
,
.
1--
,
. ,
,
,
2--2--D-,
- .

396

- .

,
2025 Polamat-A (l = 546 ).
Sorbfil-- (, ).
(254 ), 5%
200300 .
: , 5:1 (),
, 10:1 (), , 15:1 ().
() Merck 230400 .
1
Varian Mercury-400 (400 ),
Varian Mercury-300 (300 ), Me4Si.
() (.., d-) -
(, J, ).
1
2--3,4,6----1--(2-)-2---d (2)
COOH
OH

OAc
O

AcO
AcO

AcNH

OAc

K2CO3 , 15K5

CH3CN

Cl

AcO
AcO

HO

O
AcNH

1. 400 (1,10 ) - 1, 151 (1,10 )


, 151 (1,1 ) 48 (0,22
) 155 12 20
22 - (, ).
, (25 ),
. 2
,
(100:1) (30:1). 2-3,4,6----1--(2-)-2---D- 355 (69%); .. 142 , []546 50 (c 1,0; ).
1
(DMSO-d6, 300 ): 1,75 (3H, NAc), 1,96 (3H, OAc), 2,00c (6H,
2OAc), 4,12 (4H, -2, -5, H-6a, H-6b), 4,97 (1, -4, J4,5 10,2 ), 5,27 (1, 3, J3,4 10,8 ), 5,92 (1, -1, J1,2 8,4 ), 6,96, 7,01, 7,55, 7,68, (4, .),
8,09 (1, N, J2,NH 9,3 ), 10,19 (1, ).
2. 1 400 (1,1 ) - 1
2 205
(40%) .

397

.., .H., .., ..

2
(2--3,4,6----2---D-)-2-(2-3,4,6----2---D-) (3)
(2--3,4,6----2---D-)-2-(2-3,4,6----2---D-) (4)
AcNH
COOH
OH

OAc
O

AcO
AcO

AcO
AcO

CH3CN

AcNH
Cl

OAc

K2CO3 ,15K5

OAc

OAc
+

O
AcNH

AcO
AcO
OAc

OAc
OAc

AcO
AcO

AcNH
O

O
AcNH

500 (1,37 ) - 1, 94,4 (0,68 )


, 517 (3,75 ) , 11 (0,05 ) 155
15 2022
- (, ). 3 4
1. 3
(: 100:1
, 15:1) 45 (8%); .. 134 , []546 37,5 (c 0,9; ).
1
(DMSO-d6, 400 ) 3:1,76, 1,78 (6, NAc), 1,97, 1,98c,
2,01c, 2,02c (12H, 4OAc), 2,00 (6H, 3OAc), 4,11 (8H, 2 H-2, 2 H-5, 2 H-6a, 2 H-6b),
4,93, 4,96 (2H, 2 H-4, J4,5 9,6 , J4,5 10,0 ), 5,28 , 5,25 (2H, 2 H-3 J3,4 9,6
), 5,54, 5,92 (2H, 2 H-1, J1,2 8,8 ), 7,13, 7,28, 7,59, 7,74 (4, .),
7,95, 8,06 (2H, 2 N, J2,NH 8,0 , J2,NH 9,6 ).
4
(100:1)
(30:1) 38 (7%); .. 211 , []546 34 (c 1,0;
).
1
(DMSO-d6, 400 ) 4: 1,72, 1,81 (6H, 2NAc), 1,96 (9H,
3OAc), 1,99, 2,01c, 2,02 (9H, 3OAc), 4,18 (8H, 2-2, 2-5, 2H-6a, 2H-6b), 4,97,
5,04 (2, 2-4, J4,5 9,6 ), 5,28 (2, 2-3, J3,4 9,6 ), 5,59, 6,17 (2, 2-1, J1,2
3,2 , J1,2 8,4 ), 7,22, 7,39, 7,64, 7,89 (4, .), 8,00, 8,02 (2, 2N, J2,NH
8,0 , J2,NH 8,4 ).
3
(2--3,4,6----2---D-)-2-(2-3,4,6----2---D-) (3)
AcNH
OAc

OAc
O

AcO
AcO

AcNH Cl

AcO
AcO

OAc
O
O

COOH K CO ,15K5
2
3
CH3CN

AcNH

AcO
AcO

O
O
AcNH
3

398

OAc
OAc
OAc

200 (0,55 ) - 1, 256 (0,55 ) --2-3,4,6----2---D-, 76 (0,55 )


, 24 (0,11 ) 155 6
2022
- (, ). 3 1,

(100:1) (25:1). 3
255 (58%); .. 133 , []546 53 (c 1,0; ).


[15], 2--3,4,6---2---D- -
. 2
- 1, ,
20% (.) 155 ,
. 3 (). 2,
, 69%.
,
1 2 6
40%. , 155
,
, - (. 1).
1:
1: 2 : K2CO3 : 155 = 1:1:1:0,2
2:
1: 2: K2CO3 = 1:1:0,2

. 1. 2 .
2 -.
1
5,9 ..
10,2 .. 1,2--
N- 8,4 .
, - N-

399

.., .H., .., ..

,
6,95, 6,99, 7,55 7,69 ..
N-

1 . 1
- 3 4 34 .
3 4 8% 7%, ,
5 6 (. 2).
4 - , ,
.
OAc

OAc
AcO
AcO

OAc

AcO
AcO

AcNH OAc

AcNH
5

. 2. 1-- 5 6.
- 3 4 1 .
-

.

6,2 .. 3,2 , - 4. 1,2--
N- - 3
-
5.9 .. 8,8 .
3 4
2.
3 -2--3,4,6----2---D-
3 -
(
) 58%.

1.

2.

23
CH3CN 155

.
155,
,

400

3.

1--
.
- - .

1.
2.
3.
4.
5.

Glycosylation of mycotoxins / S. Grabley., M. Garies, W. Bckers, J. Thiem // Synthesis. 1992.


Vol. 27, 11. P. 10781080.
Bliard C. Glycosylation of acids under phase transfer conditions. Partial synthesis of saponins / C. Bliard,
G. Massiot, S. Nazabadioko // Tetrahedron Lett. 1994. Vol. 35, 33. P. 61076108.
Loganathan D. Phase-transfer catalysed glycosylation. Synthesis of 1-O-(p-methoxycinnamoyl)-2,3,4,6tera-O-acetyl-b-D-glycopyranose / D. Loganathan, A. Amonkar, G. Trivedi // Indian J. Chem. 1983.
Vol. 22, 4. P. 400401.
- b- N- /
.. , .. , .. , .. [ .] // . 2001. . 27,
6. . 434438.
.. : N- /
.. , .. , .. // . . . iii 2009. T. 7, 3. . 5763.

.. /
.. , .. , .. , .. //
. .. . , . 2011. . 24 (63), 2.
. 396-401.

15--5

2--3,4,6----2---D . - -
, 1 .
: , -, , .
Chupakhina T.A. Features of the phase transfer glycosilation of salicylic acid / T.A. Chupakhina,
U.N. Goncharenko, V.O. Kuryanov, A.A. Astrakhantseva // Scientific Notes of Taurida V.Vernadsky
National University. Series: Biology, chemistry. 2011. Vol. 24 (63), No. 2. . 396-401.
In the phase transfer system "solid potassium carbonateacetonitrile" in presence of catalytic amounts of 15crown-5 reaction of peracetate -D-glucosaminilchloride with salicylic acid was studied. Conditions for
obtaining mono-and bis-carbohydrate derivatives of salicylic acid whose structure was proved by 1H NMR
spectroscopy were proved.
Keywords: phase transfer catalysis, crown-ether, glycosilation, glycosyl ether.

15.05.2011 .

401

. . .
, . 24 (63). 2011. 2. . 402-407.

544.175 + 546.66


3--1--4--5
..1, ..2, .3., .4., .5.,
..5
1

. .. , ,
- , ,
3
- ,
, ,
4
, ,
5
. .. ,
,
E-mail: vshul@crimea.edu
2


[Na(H2O)][EuL4]H2O [NBu4][EuL4] (HL = 3--1--4--5).
,
.
: , 4--5, .



[1, 2]. 4-5, -
[3]. 4-
[4],
. 3--1--4-5 (HL).
H
H3C
N

H
H3C

402

OH


3--1-4--5. ,

4--5 [5].
,
.
, -, 4-
,
b- .

(III) 3--1--4--5.

3--1--4--5
[6].
. 0,16 (4 ) 25
96 %- 0,808 (4 ) 3--1--4-5.
(20-30 ). 1
10 , ,
.
,
. 55 - 60 %
.
[Na(H2O)][EuL 4]H2O (HL = 3--1--4-5).
[NBu4][EuL4]2H2O c 40-60 %.
[Na(H2O)][EuL4]H2O (I). , %: 51,94; 4,36.
C44H42EuN8O10 , %: 51,12; 4,09.
[NBu4][EuL4]22 (II). , %: 57,33; 5,78.
C60H76N9O8Eu , %: 58,37; 6,20.
I ,
II , [NBu4][EuL4] (II).
,
, Bruker Smart APEX II,
CCD- (MoK, = 0,71073
, ) [7].

.
.
SHELXL97 [8].
. 1.
.

403

.., .., .., .., .., ..


.
5--4-,

(. 1).
1.

I II

-
,
Mr

.
()

I
[Na(H2O)][EuL4]H2O
C44H40EuN8NaO10
0,45x0,24x0,14
1015,79

16,365(1)

16,428(2)

b ()

16,365(1)

16,749(2)

c ()

16,3654(10)

Z
V(3)
()
m(-1)
d(.) (/3)
q ()

4
4383,1(3)
296(2)
1,508
1,539
3,05 - 30,37
-22 < h < 18
-23 < k < 17
-23 < l < 17

16,231(2)
4
4465,9(11)
296(2)
1,48
1,784
1,7 - 26,4
-20 < h < 20
-20 < k < 20
-20 < l < 20

11387

19921

3189

4580

0,0325
0,0851
0,991
0,080; -0,563

0,038
0,115
1,000
0,85; -0,89

R
Rw
GOF
Dr, max., min. (e-3)

I 4c 2

404

II
[NBu4][EuL4]
C60H72EuN9O8
0,180,060,04
1199,23

Iba2

,
, , 0,018
I 0,020 II.
. ,

(2,366 2,467 ; 2,373 2,462
I II),
. -
(1,257 1,241; 1,262 1,244 )
, b- .

[9].
.
[Na(H2)][EuL4]2
,
(NaO 2,817 ),
(NaN 2,870 ).
(. 2).
II
2. .

. 1. [LnL4]-
I II.

405

.., .., .., .., .., ..

. 2.
[Na(H2O)][EuL4]H2O ( I).
2.

II

EuO1

2,366(3)

2,373(4)

EuO2

2,467(4)

2,462(5)

EuO3

2,366(3)

2,374(4)

EuO4

2,467(4)

2,444(5)

O1C3

1,257(5)

1,262 (8)

O2C5

1,241(5)

1,241 (8)

O3C14

1,257(5)

1,267 (8)

O4C16

1,241(5)

1,244 (8)

406

[Na(H2)][EuL4]H2O [NBu4][EuL4] (HL = 3--1--4--5).



1.
2.
3.
4.
5.

6.
7.
8.
9.

..
/ .. , .. , ..
// . 2005. . 74. - 12. . 1194-1214.
.. - (III) / ..
, .. // . . . 2006 . 51. - 1. . 80-96.
Pettenari C. Synthesis, structure and luminescence properties of new rare earth metal complexes with 1phenyl-3-methyl-4-acylpyrazol-5-ones / C. Pettenari, F. Marchetti, R. Pettenari [et al.] // J. Chem. Soc.
Dalton Trans. - 2002. - 7. - . 1409-1415.
Marchetti F. Acylpyrazolone ligands: Synthesis, structures, metal coordination chemistry and
applications // F. Marchetti, Pettinari C., Pettinari R. // Coord. Chem. Rev. - 2005. - Vol. 249. - P. 29092945.
..
5--3--1--4- / .. , .. , ..
[ .] //
. .. . . 2009. - . 22 (61). - 4. - . 332-337.
.. 1--3--4--5 / .. , .. - // . -1964. .34, 9. . 3005-3012.
SMART (Control) and SAINT (Integration) Sofware, Version 5.0, Bruker AXS Inc., Madison(WI), 1997.
Sheldrick G.M. SHELX97. Program for the Solution of Crystal Structures / G.M. Sheldrick. - Gttingen
University, Gttingen (Germany), 1997.
Allen F.H. Tables of lengths determined by X-ray and neutron diffraction. Part 1. Bond lengths in organic
compounds / F.H. Allen, O. Kennard, D.G. Watson [et al] // J. Chem. Soc. Perkin Trans. 2 1987. Pt.
2. - 12. S. 1-19.

.. 3--1--4-5 / .. , .. , .. , .. ,
.. , .. // . ..
. , . 2011. . 24 (63), 2. . 402-407.

[Na(H2O)][EuL4]H2O [NBu4][EuL4] (HL = 3--1--4--5). ,

.
: , 4--5, .
Shulgin V.F. Crystal structure of the anionic complexes of europium with 3-methyl-1-phenyl-4formylpirazolon-5 / V.F. Shulgin, S.V. Abkhairova, O.V. Konnic, E.B. Rusanov, G.G. Alexandrov,
I.L. Eremenko // Scientific Notes of Taurida V.Vernadsky National University. Series: Biology, chemistry.
2011. Vol. 24 (63), No. 2. . 402-407.
The coordination compounds [Na(H2O)2][EuL4] and [NBu4][EuL4] (HL = 3-methyl-1-phenyl-4formylpirazolon-5) were synthesized and investigated by X-Ray analysis method. This is found that anionic
complexes have a square antiprismatic geometry and contains four residues of pirazolone.
Keywords: europium, 4-acylpirazolone-5, X-Ray analysis.

30.05.2011 .

407

. . .
, . 24 (63). 2011. 2. . 408-414.

547.918:547.859:543.42:581.142


:
..1, ..2, ..1, ..1
1

, ,
. .. , ,
E-mail: chemsevntu@rambler.ru

-
3--a-L--(12)--a-L- (a)

28--a-L--(14)--b-D--(16)--b-D ( ) - . ,
1:1.
Avena sativa L.
. , a-, , ,
, -, Avena sativa L., .


,
.

,
[1, 2].

(. 1) a- (3-a-L--(12)--a-L- , 1)
3--a-L--(12)--a-L--28--a-L--(14)--b-D--(16)--b-D-
( , 2) [3].
1 2
[47], ( [8],
[9], [10], [11, 12]) [13].
1 2 (SC,
. 1) . ,
1:1 [14]. SC
Helix albescens [15].

408

SC (,
, , IC .) (revatio)
[16, 17].
1 2 SC
- ,
Avena sativa L.
20
12
18

28
16

N
C6H8O7

5'

2'

CH3
23

HO

HN

OH

H3C
HO

COOR

CH3

HO

CH3

SO 2

OH

CH3

1''

HO

. 1.
( 1: R=; 2: R=bGlcp-(61)bGlcp-(41)-aRhap).

1 2 Hedera taurica Carr. Hedera


canariensis Willd. (Araliaceae Juss.) ,
[18, 19].
Shilpa Medicare Limited () ( NPV0080309).
- (2022 )
Unico UV-Vis 4802 () (l=1 ).
104 -
SC. (Na2HPO4NaH2PO4;
7.0) 96 % 25 4:1.
(2022 ) 40
. . 2 3.
Avena
sativa L. (Poaceae) 2728 C.
SC 1 2 4060
. SC,
, 0.5104 . ( 25 ) 5
24 .

409

.., .., .., ..

,
, .
24, 48 72 (. 1).
a=0.95.
1
Avena sativa
( 0.5104 )

24 :
, %
,
, %
,
48 :
, %
,
, %
,
72 :
, %
,
, %
,

H2O
()

SC

1SC

2SC

52
0.50.2
80
0.90.2

8
0.10.1
56
0.70.3

24
0.40.3
64
1.00.5

56
0.60.2
72
1.00.3

64
0.90.4
80
1.90.6

80
1.40.7
88
3.21.1

76
3.91.4
88
2.20.7

52
0.50.2
80
1.30.5

64
1.60.8
76
2.00.9

84
2.20.9
92
3.51.4

76
3.11.2
84
5.12.0

80
4.41.8
88
7.42.5

92
7.91.8
92
7.11.9

68
1.60.8
80
1.60.8

86
3.81.7
84
2.90.9

88
5.92.1
92
6.42.4

76
9.73.4
84
11.23.9

92
10.73.6
92
14.13.9

-.
[20]. . 2
, 1 SC
.
1.0,
1:1. , 2
SC, 1.0 (. 3). 2 SC
1:1.
1 2 SC [14]. ,
, -
.
.

, ..

410

[21]. ,
[21, 22]. SC
[23, 24].
SC.
1 2 SC
Avena sativa (. 1).
1.0

0.8

0.6

0.4

0.2

0.0
0

10

c(SC)/c(1)

. 2.
l = 291 : (SC) = 104 , (1) = 104 (
(Na2HPO4NaH2PO4; 7.0) 96 % 25
4:1).
1 (. 1).
2 . 72
, SC
,
. , SC Nicotiana tabaccum
, [23].
SC
24 48 . 72
(92 %), SC
( 92 %).
2SC . 24
80 % ( 52 %).
1, , ..
. , 2SC
.
2 .
. , 1SC
.

411

.., .., .., ..

, (II) NO ,
GMP,
[24]. SC , GMP
GMP [16, 24]. SC
.
2.4
2.2
2.0
1.8
1.6

1.4
1.2
1.0
0.8
0.6
0.4
0.2
0.0
0

10

c(SC)/c(2)

. 3.
l = 291 : (SC) = 104 , (2) = 104 (
(Na2HPO4NaH2PO4; 7.0) 96 % 25
4:1).
-
. (2010 2011 .). ..
( , . ) .

1. -
SC
.
2. , -
SC , 1:1.
3. SC 24 48
. 2SC ,
1SC .
2SC 2
.

412


1.
2.
3.

4.

5.

6.

7.
8.
9.
10.
11.
12.
13.
14.
15.

16.
17.

: , , / [.. , .. ,
.. .]. : , 2007. 311 .
.. / .. , .. ,
.. // . 2007. . 77, 10. . 867-874.
Molecular complexation of ivy saponins with some drugs and biologically active substances /
L.A. Yakovishin, V.I. Grishkovets, G. Schroeder, N.I. Borisenko // Functionalized molecules synthesis,
properties and application; ed. V.I. Rybachenko. Donetsk: Schidnyj wydawnyczyj dim, 2010. Chapter
4. P. 85103.

/ .. , .. , .. [ .] //
. .. . , .
2010. . 23 (62), 2. . 270275.
L-
/ .. , .. , .. [ .] //
. .. . , .
2010. . 23 (62), 3. . 255261.
L-
/ .. , .. , .. [ .] //
. .. . , .
2011. . 24 (63), 1. . 232238.
- - L / .. , .. , .. [ .] // . . . 2011.
. 78, 4. . 535540.
- -
/ .. , .. , .. [ .] // . . . 2010.
. 77, 5. . 668672.
() a-
/ .. , .. ,
.. [ .] // -. 2011. . 8, 2. . 111114.
X . (, ),
27 . 2010 ., --. --: . . . 2010. . 29.
.. a-
/ .. // Ukr. Bioorg. Acta. 2010. . 8, 1. . 4246.
..
/ .. // . . 2010. 5. . 629631.
/ .. ,
.. , .. [ .] // . . 2010. 1. . 4548.
VII . -. .
. 2011, 2630 . 2011 ., . : - . 2011.
. 269270.

Helix albescens / .. , .. , .. [ .] //
. .. .
, . 2010. . 23 (62), 2. . 96103.
Drewes S.E. Recent findings on natural products with erectile-dysfunction activity / S.E. Drewes, J.
George, F. Khan // Phytochemistry. 2003. Vol. 62. P. 10191025.
Guidelines for the diagnosis and treatment of pulmonary hypertension. The task force for the diagnosis
and treatment of pulmonary hypertension of the european society of cardiology (ESC) and the european
respiratory society (ERS), endorsed by the international society of heart and lung transplantation (ISHLT)
/ N. Galie, M.M. Hoeper, M. Humbert [et al.] // Eur. Heart J. 2009. Vol. 30. P. 24932537.

413

.., .., .., ..


18. Hedera taurica I. Hedera taurica /
.. , .. , .. [ .] // . . 1987. 3.
. 363366.
19. Hedera canariensis I. L-A, L-B1, L-B2, L-C, L-D, LE1, L-G1, L-G2, L-G3, L-G4, L-H1, L-H2 L-I1 Hedera canariensis / .. ,
.. , .. [ .] // . . 1996. 3. . 377383.
20. .. / .. ,
.. . [5- .]. .: , 1986. 432 .
21. .. / .. , .. //
. 1980. . 6, 3. . 351364.
22. Podolak I. Saponins as cytotoxic agents: a review / I. Podolak, A. Galanty, D. Sobolewska // Phytochem.
Rev. 2010. Vol. 9. P. 425474.
23. Vaidyanathan P. Impact of sildenafil citrate on tissue culture of tobacco (Nicotiana tabaccum) / P.
Vaidyanathan, K. Francis // Plant Cell Biotech. Mol. Biol. 2009. Vol. 10, No. 1/2. . 4956.
24. Pagnussat G.C. Nitric oxide and cyclic GMP are messengers in the indole acetic acid-induced
adventitious rooting process / G.C. Pagnussat, M.L. Lanteri, L. Lamattina // Plant Physiol. 2003. Vol.
132. . 12411248.
..
: - / .. ,
.. , .. , .. //
. .. . , . 2011. . 24 (63), 2. . 408-414.
-
3--a-L--(12)--a-L- (a-)
28--a-L--(14)--b-D--(16)--b-D-
( ) - . ,
1:1.
Avena sativa L.
. , a-, , ,
, -, Avena sativa L., .

Yakovishin L.A. Supramolecular complexes of the triterpene glycosides with sildenafil citrate:
formation in aqueous-alcoholic solutions and biological activity / L.A. Yakovishin, V.I. Grishkovets,
D.Yu. Belash, I.R. Yarovoy // Scientific Notes of Taurida V.Vernadsky National University. Series: Biology,
chemistry. 2011. Vol. 24 (63), No. 2. . 4-08-414.
Using a method UV-spectroscopy, the molecular complexation of sildenafil citrate with hederagenin 3-O-a-Lrhamnopyranosyl-(12)-O-a-L-arabinopyranoside (a-hederin) and its 28--a-L-rhamnopyranosyl-(14)-b-D-glucopyranosyl-(16)--b-D-glucopyranosyl ester (hederasaponin C) in aqueous-alcoholic solutions
was for the first time investigated. It was found that the glycosides form complexes with sildenafil citrate in
the 1:1 molar ratio. Comparative study of influence of glycosides and complexes on seeds germination Avena
sativa L. has been made.
Keywords. triterpene glycosides, a-hederin, hederasaponin C, sildenafil citrate, supramolecular complex, UVspectroscopy, Avena sativa L., seeds germination.

20.02.2011 .

414

. ..
, ,

. .. ,

, e-mail: mermaid.ka@mail.ru

,
, , . (0692)24-01-87, -mail: nataliy-andreev@yandex.ru

. .. ,

. ..,
, , . (0652) 251666

. .. ,
,
. (0652) 602477, e-mail: shapaa86@mail.ru

. .. ,

. .. ,

, . (0652) 602477

. .. ,
,
, . (0652) 602477, e-mail: b_m_y@mail.ru

- ,
, ,
, ,
e-mail: kipubiology@gmail.com.

415

- ,
3 -

-
, ,

, -mail: olgabelyaeva@yandex.ru

- . ,

, -mail: Bokal@list.ru

. .. ,
,


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( 50-

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......................................................... 22


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....................................................................... 30
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(TURSIOPS TRUNCATUS),
.................................... 50
..


...........................................................................62
..

( ) ............................... 67
.., - ..
IN VITRO. .......................................... 73
..

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..

,
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425

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(SAR) ---D-
.....................143
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. ..........................................150
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..................157
..

...............................................................161
..
HELIX ALBESCENS
............................................................................................169
..

PINUS SYLVESTRIS .............................................................................179
.., ..
IN VITRO LAVANDULA ANGUSTIFOLIA MILL. .........186

426

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............. 195
..


........................................ 203
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.......................................................................................................... 214
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- (INSECTA,
ORTHOPTERA) ....................................................................................... 231
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.............. 240
..

.......................... 249
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...................................... 254
Sobko V.M., Martynyuk V.S., Shevchenko V.B., Protopopov M.V.
EFFECT OF ELECTROMAGNETIC FIELD WITH 8 Hz FREQUENCY ON
THYMOCYTES NUCLEUSES INJURY CAUSED BY NANOSTRUCTURED
SILICON AND HYDROGEN PEROXIDE................................................................ 261
.., .., .., ..


DUGESIA TIGRINA........................................................................... 268
.., .., .., ..
CLEMATIS VITALBA
, ............................... 279

427

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(MELILOTUS OFFICINALIS (L.) PALL.)
...................................................................................................284
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L .....291
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24 ..................................................................................................298
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...................304
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FATSHEDERA LIZEI
......................................................................313
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5-(2'-)-2-(2'-)-7,8--6,5--1,3,6
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.................................................................360

428

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ALLIUM CYRILLII (ALLIACEAE)............................................................................ 390
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.......................................... 396
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3--1--4--5 ........................................ 402
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............................................. 408
.................................................................................. 415
.................................................................................................... 425

429