Industries-Cellulase is used for commercial food processing in coffee.

It
performs hydrolysis of cellulose during drying of beans. Furthermore, cellulases are
widely used in textile industry and in laundry detergents.
Substrate-As the native substrate, cellulose, is a water-insoluble polymer, traditional
reducing sugar assays using this substrate can not be employed for the measurement of
cellulase activity.
Catalytic action
Five general types of cellulases based on the type of reaction catalyzed: (EC
3.2.1.4)=kegg enzyme

Endocellulases (EC 3.2.1.4) randomly cleave internal bonds at amorphous sites

that create new chain ends.

Exocellulases or cellobiohydrolases (EC 3.2.1.91) cleave two to four units from

the ends of the exposed chains produced by endocellulase, resulting
in tetrasaccharides[5] or disaccharides, such as cellobiose. Exocellulases are
further classified into type I, that work processively from the reducing end of the
cellulose chain, and type II, that work processively from the nonreducing end.

Cellobiases (EC 3.2.1.21) or beta-glucosidases hydrolyse the exocellulase product

into individual monosaccharides.

Oxidative cellulases depolymerize cellulose by radical reactions, as for

instance cellobiose dehydrogenase (acceptor).

Cellulose phosphorylases depolymerize cellulose using phosphates instead of

water

Celluslase produced by the natural fermentation process of Trichoderma reesei and

Bacillus licheniformis.