Академический Документы
Профессиональный Документы
Культура Документы
LOCATION:
Cuddalore District has an area of 3,678 km (coastal line of 68 kms) with the human population of
26,00,880(Provisional 2011 Census). It is bounded on the north by Viluppuram District and Pondicherry, on the
east by the Bay of Bengal, on the south by Nagapattinam District, and on the west by Perambalur District.
GEOGRAPHICAL POSITION:
North Latitude between 15o 5'/11o 11 and 12o 35
East Longitude between 78 o 38 and 80o
SOIL TYPE:
Loamy soil, Clayey soil,
Alluvial soil, Sandy loam and
Sandy clay soil are the soil types found in the district
RAINFALL (in mm)
Normal
1. N E Monsoon : 716.5
2. S W Monsoon : 373.6
TEMPERATURE IN THE DISTRICT
1. Maximum : 36.8 C
2. Minimum : 19.9 C
Cuddalore District:
Revenue Divisions
Revenue Taluks
Revenue Villages
Township
Municipalities
Town Panchayats
Blocks
Panchayat Villages
:3
:8
: 896
:1
:5
: 16
: 13
: 681
a) Dairies
: Nil
b) Milk chilling plant
:1
c) No.of milk Co-op. Societies
: 237
d) Milk Production Per Day (in Liters) :1,30,16,265
CUDDALORE MAP
(Cuddalore District)
:1
:1
: 10
3. Assistant Directors
a. Animal Disease Intelligence Unit
(1)
b. Cuddalore Division
(1)
c.ChidambaramDivision
(1)
d.Viruthachalam Division
(1)
e. Clinician
(1)
f. Veterinary Surgeons
(5)
: 81
(2)
(2)
c. Cuddalore Division
(22)
d. ChidambaramDivision
(29)
e. Viruthachalam Division
(24)
f. Clinician Centre
(1)
g. VH. Melpattampakkam
(1)
No of Poly Clinic
: -Nil-
No of Clinician Center
:1
No of Veterinary Hospital
:5
No of Veterinary Dispensaries
: 60
:2
: 13
No of Sub-Centers
: 64
15.05.1978
3. Staff
post
Sanctioned
Filled
Vaccant
Assistant Director
Assistant
Lab Assistant
Driver
Lab Attender
A.H. Assistant
As per the Head office R.O.C. No. 35517/A1/78 Dt. 5.8.1978, Cuddalore Clinical Lab with one Veterinary
Asst. Surgeon, one Lab Attender and one A.H.Assistant was attached to this Unit.
Till 30.08.2007, ADIU was functioning at the first floor of the Clinical Block of Veterinary Hospital. Later
on, Permanent building was constructed with the financial assistance of NABARD X scheme with the estimate of
28.4 lakhs.
Sl.No
Name
Remarks
1.
2.
Autoclave
3.
Mini Autoclave
4.
Magnetic stirrer
with thermostat
5.
Digital pH meter
6.
7.
Semi Analyzer
8.
Auto haemo
analyzer
To prepare solution
To find PH of Various Reagents
To test for Brucellosis, media inoculation,
Culture preparation / ABST
For analyzing serum
For whole blood analysis
9.
Urine Analyzer
10.
Centrifuge
11.
Water bath
12.
Bottle cooler
13.
Deep freezer
14.
Walk in cooler
15.
CIE
16.
Incubator
2) To carry out Epidemiological surveillance work and collection of data regarding Outbreaks of Disease
both in endemic and in non-endemic areas and prepare Endemic Charts.
3) To keep close watch of Emerging Diseases of Livestock and Poultry (Bird Flu, Leptospirosis, Blue
tongue, Bovine Viral Diarrhoea, Infectious Rhino Tracheitis, Mycoplasmosis, Brucellosis, Infectious
Bursal Diseases, etc)
4) Forecasting outbreaks of Contagious and Infectious diseases and relaying the information well in
advance to the field staff for preventive measures and also to the farmers through Mass media.
5) Mass screening of milch animals in Organised dairy farms for Mastitis Brucellosis, Tuberculosis and
Johnin disease.
6) Examination of clinical materials and specimen samples received from the field staff and
communication of the result then and there.
7. To attend outbreak reports where diagnostic aids are needed from this unit and advice remedial
measures.
8. Semen evaluation works where ever applicable.
9. To study the migratory trends of cattle, sheep, Poultry etc, and its basic reasons and relevance for the
disease containment programme.
10. Collection of climatological data and study their effect on occurrence of diseases, helminthic problems
and ecosystem.
11. To examine the animals and carcasses periodically at the slaughter houses and assure the disease
position.
12. To study toxicological problems including aflotoxicosis, Poisoning due to pesticide, plants, industrial
effluents, sewage pollution etc.
13. To keep Buffer Stock Vaccines for urgent use.
14. Collecting Pre and Post serum samples from animals for efficacy testing of vaccine and antibody
demonstration of Foot and Mouth Disease.
15. To take part in all activities related to Preparedness, Control and Containment of Avian Influenza.
16. Sero monitoring, Clinical surveillance & Sero - Surveillance of Avian Influenza.
17. To attend Mass Contact Programme including Kalnadai Pathukappu Thittam .
TARGET
TARGET
(2013-14)
ACHIEVED
13500
14750
2.
Blood Smear
350
831
3.
40
69
4.
Impression Smear
400
458
5.
Intestinal Scrapings
150
194
6.
Skin Scrapings
500
544
7.
Nasal Washing
70
91
8.
70
145
9.
90
178
10.
Urine Analysis
150
210
11.
Semen Analysis
120
147
12.
120
237
13.
250
282
14.
120
1276
15.
ABST
350
555
16.
46
17.
20013
ANIMAL TESTING
1.
150
295
III.
GENERAL
1.
2.
120
443
3.
135
4.
44
5.
12
a. Government Farms
b. Private Farms
25
6.
50
64
7.
58
8.
250
316
9.
250
265
10.
200
87
11
200
258
II.
44
Amphistomes
Ascaria
Trichiuris
Toxacara
Ancylostoma
Coccidia
Fasciola
Others
187
144
59
155
10
83
75
75
287
P.multocida
Trypnosoma
E.canis
Babesia
Theileria
Kochs-Blue
Anaplasma
Others
ESR
PCV
WBC
Hb
TC
DC
--
3.Blood analysis
Microfilaria
Anthracis Bascilus
2.Blood smear
--
--
67
5
2
53
--
56
--
10
--
63
10
75
4.SKIN SCRAPINGS
Psoroptes
Sarcoptes
29
Demodex
39
68
5.URINE SAMPLES
Trichophyton
Microsporum
--
--
Bile
Albumin
Ketones
Blood
Glucose
Sediments
Pigments
Bile
salts
10
16
71
23
DUNG
BLOOD
SMEAR
URINE
SAMPLES
BLOOD
ANALYSIS
SKIN
SCRAPING
S
IMP.
SMEAR
MILK
NASAL
WASHINGS
Total
Positiv
e
Total
Positiv
e
Total
Positiv
e
Total
Positiv
e
Total
Positive
Total
Positiv
e
Total
Positiv
e
Total
positive
14750
1075
801
230
210
125
323
158 +
544
136
458
59
237
126
91
Jhonin Testing
Semen
Evaluation
Brucellosis
Intestinal
Scrapings
Electrophoro
sis
ABST
Test For
Infertility
Total
Positive
Total
Positive
Total
Positive
Total
Positive
Total
Positive
Total
Positive
Total
Positive
--
--
282
147
194
44
555
443
295
##
4. Tour Details
Panchayat
union
Villages
Visited
OBR
attended
Visited
135
443
Specimens send to
Beneficiaries
Slaughter
house
specimens
No. of
Post
mortem
Specimens
Examined
IVPM
MVC
CRL
SC/S
T
OTHERS
314
58
60
20
283
2315
4362
44
TOTAL
6677
2011 -12
2012 -13
2013 -14
2014-15
ASV
92750
68000
87500
32500
39600
BQV
28000
42500
67500
70600
59900
HSV
2500
1500
250
56500
4500
PPRV
NIL
100300
183000
19800
81800
FMDV
300000
59000
477000
669000
703000
ETV
2250
8000
2000
3000
2500
RDVK
268800
581600
536600
973800
1052000
Name of
the
vaccine
Dosses to be
lifted
No.Vaccinated
Remarks
Month of
Vaccination
Name of the
block
Anthrax
25000 ml
31972
Vaccine fully
utilized
Aug-13
Kammapuram
Black
Quarter
17700 doses
17692
Vaccine fully
utilized
Sep-13
Kammapuram
Black
Quarter
20000 doses
19968
Vaccine fully
utilized
Oct-13
Panrutti
Black
Quarter
27000 doses
26989
Vaccine fully
utilized
Nov-13
Nallur
S.N
o.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
Kind of specimen
Dung sample
Blood smear
Blood wet film
Impression smear
Intestinal scraping
Skin scraping
Nasal washing
Blood analysis
Blood biochemistry
Urine analysis
Semen analysis
Milk sample for mastitis
Brucellosis test-serum sample
Brucellosis test-milk sample
ABST
Other specimens
Total tested at ADIU
No. sent to CRL-MVC-SRDDL etc
18
includes Birdflu
19
No.of sera sample sent to CRL for AI
Total No.specimens- Received by
ADIU ( 17 + 18 )
ANIMAL TESTING
II
1
No. of animals tested for TB
2
No. of animals tested for JD
3
No.of Infertility
III GENERAL
1
No.of OBR attended
2
No.of villages visited
3
No: of Panchayat Union covered
4
No.of MCP'S attended
5
Visits made to organised farms
a) Govt.Farm
b) Private farms
Bar
Target
13500
350
40
400
150
500
70
70
90
150
120
120
250
120
350
Achievement
15343
685
60
436
197
550
85
261
250
276
55
277
299
240
655
58
19730
50
90
150
120
19870
0
500
0
291
0
0
518
135
56
12
0
25
0
0
111
50
71
53
250
330
250
254
10
200
242
11
200
159
Dung sample
15500
15000
14500
Dung sample
14000
13500
13000
12500
target
achievement
700
600
500
400
300
200
100
0
target
achievement
No.of
Animal
Doses Lifted Vaccinated
S.N
o
Name of the
vaccine
1
2
3
4
Antharax
Blackquarter
ETV
HSV
39600 ml
59900
2500
4500
48881
59400
2392
4385
* ASCAD
ASCAD
Buffer Stock
Buffer Stock
FMD
350000
350350
FMD-CP VIIPhase
FMD
353000
350340
FMD-CP VIIIPhase
FMD Total
703000
700690
PPR
81800
78800
FMD-CP - 2014-15
NCP-PPR & Scheme animal
vaccination
RDVK
1052000
1032000
Annual Target
Brucella
21000
21000
NCPB
Remarks
1. DUNG
Strongyl
es sp.
Amphisto
mes
Asca
ria
Trichiu
ris
Toxaca
ra
Ancylosto
ma
Coccid
ia
Fasciol
a
Other
s
318
117
61
178
33
49
112
36
329
DUNG
Gluc
ose
47
--
27
25
70
BLOOD
SMEAR
URINE
SAMPL
ES
BLOOD
ANALY
SIS
SKIN
SCRAPI
NGS
IMP.
SMEAR
80
11
7
MILK
DC
Blo
od
ESR
Micr
Bile
Bil Albu
Keto
oe
min
nes
40Pigm47 sa 2
53
-69
spor
-ents
um
lts
5.URINE SAMPLES
TC
Others
Hb
--
WBC
79
PCV
40
27
Tric
ho6
54phyt
2
on
Anaplasma
Demo
dex
3. Blood analysis
Kochs-Blue
Sarco
ptes
Theileria
Babesia
E.canis
--
Trypno-soma
P.multo-cida
--
4.SKIN SCRAPINGS
Psoro
ptes
--
Microfl-aria
Anthracissss
Bascilus
2. Blood smear
Sedim
ents
14
0
92
NASAL
WASHI
NGS
Tot
al
Posit
ive
Tot
al
Posit
ive
Tot
al
Posit
ive
Tot
al
Posit
ive
Tot
al
Positi
ve
Tot
al
Posit
ive
Tot
al
Posit
ive
Tot
al
positi
ve
153
43
1233
68
5
221
27
6
175
51
1
366
55
0
146
43
6
317
27
7
165
85
Johnin
Testing
Brucello
sis
SemenEvalu
ation
Intestinal
Scrapings
ABST
Electropho
rosis
Test For
Infertilit
y
Tota
l
Positi
ve
Tota
l
Positi
ve
Total
Positi
ve
Total
Positi
ve
Tota
l
Positi
ve
Total
Positive
Tot
al
Positi
ve
--
--
52
6
55
197
48
65
5
605
29
1
##
Pancha
yat
union
Visited
Villag
es
Visite
d
135
518
OBR
attend
ed
Slaught
er
house
specim
ens
No. of
Post
mortem
Specim
ens
Examin
ed
330
53
Specimens
send to
Benefciarie
s
IVP
M
MV
C
CR
L
SC/
ST
OTHE
RS
600
50
284
4
4612
TOT
AL
7456
Amphistomes
Ascaria
Trichiuris
Toxacara
Ancylostoma
Coccidia
Fasciola
Others
Trypnosoma
18%
E.canis
1%1%
Babesia
21%
Theileria
26%
27%
Kochs-Blue
15%
Anaplasma
24%
3%
9%
4%
Others
20%
9%
5%
3%
14%
Urine Samples
Glucose Sediments
18%
Psoroptes
Blood
Sarcoptes
Albumin
Demodex
54%
Bile pigment
27%
Ketones
Total Positive
7%
Total collected
samples
24%
negative
39%
Positive samples
61%
Positive
76%
93%
IMPRESSION SMEAR
21%
42% Total
Positive
Total
Positive
Positive; 42%
Total; 58%
58%
79%
NASAL WASHINGS
MILK ANALYSIS-CMT
Intestinal Scrapings
positive; 1%
20%
Total
Positive; 37%
Positive
Total; 63%
80%
Total; 99%
S.NO
YEAR
&MONT
H OF
OBR
Name of the
Disaese
Villages Affected
Name of the
Block
Name of the VD
Aug-07
Antharax
Visalur
Viruthachalam
Mangalampettai
Sep-07
FMD
Chinnavadavadi
Viruthachalam
Viruthachalam
Sep-07
FMD
Vadakuppam
Viruthachalam
Viruthachalam
Sep-07
FMD
Abathanapuram
Kurinjipadi
Vadalur
Oct-07
FMD
Keelpattampakkam
Annagramam
Nellikuppam
Oct-07
FMD
Muttam
K.M.Koil
Muttam
Oct-07
FMD
Karuperri
K.M.Koil
Muttam
Oct-07
FMD
Azangatthan
K.M.Koil
Muttam
Oct-07
FMD
Vallampadugai
Kumaratchi
Vallampadugai
10
Oct-07
FMD
Vadakkumankudi
Kumaratchi
Vallampadugai
11
Oct-07
FMD
Melakundalapadi
Kumaratchi
Vallampadugai
12
Oct-07
Enterotoxemia
Thachur
Mangalore
Thozudur
13
Oct-07
Enterotoxemia
Korrukai
Mangalore
Thozudur
14
Oct-07
Enterotoxemia
Sirugramam
Panrutti
V.P.Nallur
15
Nov-07
FMD
Orangur
Mangalore
Orangur
16
Nov-07
FMD
Keeranur
Kammapuram
V.Kumaramangalam
17
Nov-07
FMD
Tholar
Kammapuram
V.Kumaramangalam
18
Jan-08
Black Quarter
Maligaikottam
Thittakudi
Pennadam
19
Mar-08
PPR
Thoppukollai
Kurinjipadi
Kullanchavadi
20
Nov-09
Antharax
Karmankudi
Kammapuram
Kammapuram
21
Nov-12
Black Quarter
Ammrei
Kammapuram
Mandarakuppam
22
Dec-12
Black Quarter
Kudimiyankuppam
Panrutti
V.P.Nallur
23
Dec-12
Sep & oct
-13
Black Quarter
Thachampalayam
Panrutti
Marungur
FMD
43 villages
8 blocks
14 - VD
24
MONTH
January
February
March
April
May
June
July
August
September
October
November
December
Anthrax
BQ
FMD
ET
PPR
Duck
plague
Goat
Pox
Kumaratchi
5
6
Parangipettai
7
8
9
1
11
Kattumannarkoil 1
1
3
1
1
1
1
Keerapalayam
18
19
20
21
22
23
24
25
26
27
28
29
Melbhuvanagiri 30
31
32
V.D Vallambadugai
V.D Kumaratchi
V.D Sivayam
V.D Lalpettai
R.V.D Ammapettai
Puliyangudi
V.D Kavarapattu
V.D Pinnathur
V.D B.Mutlur
V.D Parangipettai
R.V.D Sendirakillai
V.D Thirumuttam
V.D Vilvakulam
V.D Kattumannarkoil
V.D Kanjankollai
V.D Muttam
R.V.D Movur
V.D A.Puliyangudi
V.D Vilagam
V.D T.Nedunjeri
V.D Veiyalur
V.D Orathur
V.D Cholatharam
R.V.D Kannangudi
Perur
V.D C.Mutlur
V.D Pinnalur
V.D Sethiyathope
V.D Chidambaram
R.V.D Melvalaiyamadevi
maruthur
M.V.D Chidambaram
Karuppur
Thirunaraiyur
Meiyathur
Parivilagam
Neivasal
Ma.Udaiyur
Thiruvakulam
Nakaravanthankudi
Thillaividagan
Puduchatiram
Gunamangalam
Vanamadevi
Pazhanjanallur
Kollumedu
Chettithangal
Rediyur
Keerapalayam
Mugaiyur
Thenpathi
Nangudi
C.Alambadi
Veeramudaiyanatham
Manjakollai
26
5
1
23
2) Vridhachalam Division:
Veterinary Dispensary, Rural Veterinary Dispensary &Sub Centre Details Blockwise
Block
Vridhachalam
Kammapuram
Mangalore
Nallur
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
V.D Vridhachalam
V.D Karuvepilankurichi
V.D Mangalampettai
V.D Sathukoodal Keelpathy
R.V.D Mathur
V.D T.Gopurapuram
Kammapuram
V.Kumaramangalam
Mandarakuppam
R.V.D U.Mangalam
Kavanur
Iruppu
Kotteri
Mangalore
Thozhudur
Avatti
Avinangudi
Orangur
R.V.D Venganur
Nallur
Pennadam
Periyanesalur
Tholar
Veppur
R.V.DDheevalur
M. V.D Vridhachalam
Thoravalur
3) Cuddalore Division:
V.Sathapadi
C.Keeranur
Adari
Keezhakalpoondi
Edaicheruvai
Me.Mathur
Kothattai
Kovilur
Pelandurai
21
4
1
10
Block
L.N.Puram
Maligampattu
Perperiyankuppam
Vegakollai
Kadampuliyur
Thiruvamur
Kalliyankuppam
Panruti
Anguchettypalayam
Kurinjipadi
2
3
4
5
6
7
8
9
10
Kudiyiruppu
Marungur
R.V.D Pathirakottai
V P Nallur
Adooragaram
Kullanchvadi
puliyur
R.V.D Maruvai
Mettupalayam
11
Neyveli
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Vadalur
C N Palayam
Cuddalore O T
R.V.D Kannarapettai
Pudhukadai
Karamanikuppam
Thirupapuliyur
Ramapuram
Kandrakottai
Karumbur
Nellikuppam
Pagandai
R.V.D Thorapadi
MVD Cuddalore
Cuddalore
Annagramam
Ramanathankuppam
Thampipettai
Kothandaramapuram
Poondiankuppam
Thethanagiri
Venkadampettai
Vadakuthoo
Thiruvanthipuram
Sedapalayam
Thookanampakkam
Varakalpattu
Keelalingipattu
Periakankanamkuppa
Panapakkam
Pallur
Melkumaramangalam
Sathipattu
20
4
24
1
Chapter- 1
PPR- An Emerging and Economically Important Disease Of Small Ruminants-An overview
Dr.V.S.Raghavan, M.V.Sc., Assistant Director, Animal Disease Intelligence Unit, Cuddalore
Introduction:
PPR (Peste des Petits Ruminants) outbreak in past occurrence in cuddalore region is encountered in
the months of March. Climatic pattern of cuddalore at the end of March embarks summer season, with the
mercury reaching its peak by the end of May and June.Summer rains are sparse and the first monsoon,
the South-West monsoon, sets in June/July and continues till September. North-East monsoon sets in
October and continues till January. The rainfall during South-West monsoon period is much lower than that of
North-East monsoon. However the pattern of PPR disease occurrence in india and worldwide varies pertinent to
different ecological systems and geographical areas.
PPR mainly affects sheep and goat, though the severity of the clinical symptoms is more
predominant in goats worldwide. However in india its severity impinge both on Goats and sheep. The disease
was first reported in the Ivory Coast of West Africa by Gargadenec and Lalanne (1942). In India, PPR first
reported in 1987 from Arasur village of Villupuram district of Tamilnadu. Over a decade of serosurviellance
corroborate one out of the three small ruminants is exposed to PPRV infection.
Since the small ruminants are chiefly the livelihood for most small farmers hence PPR emerging
in developing country is menace to the poor families and eventually to state and India.
Causative Agent:
PPR is caused by Morbillivirus in the family Paramyxoviridae (Gibbs et al., 1979). Virus members of
this group have six structural proteins. Since the virus is enveloped one, it is easily inactivated under sunlight
and with many chemicals. The genome of PPRV is a negative sense, singlestranded RNA with the size of
15948 bp.
Epidemiology:
PPR infection high positivity was encountered in Tamilnadu (78.6) compared to other states. PPR
outbreaks among sheep and goats in india can occur at any time of the year, but its occurrence is most frequent
in the wet season (September to November) and cold dry (December to February).
Clinical Signs:
Clinical pictures of PPR are almost analogue to rinderpest of cattle.
1. Clinical signs of PPR could be characterized by 3Ds i.e. discharge, diarrhoea and death, with additional
fourth major components that is the bronchopneumonia.
2. After a 57 days incubation period, the affected animals exhibit 105107 F followed by a frothy nasal
discharge and lacrimation.
3. Oral mucosa shows necrotic spots, oedematous lips and bran like deposits on the tongue.
4. With the progression of the disease, the animals develop diarrhoea, which usually appears 23 days after
the onset of fever.
5. Diarrhoea results in dehydration of the animal leading to sunken eyeballs. The animal either succumbs to
death due to respiratory problems within 710 days from the onset of the clinical reaction or recovers after
a protracted convalescence.
Note :
PPR infection severity exacerbate by secondary bacterial infection with Pasteurella Multocida in lungs.
Postmortem Lesion:
1. Prominent crusty scabs along the outer lips
2. Severe interstitial apical pneumonia
3. Erosive lesions that may extend from the mouth to the reticulorumen junction, characteristic linear
haemorrhages or zebra stripes occur in the large intestine. commonly at the caecocolic junction,
4. Necrotic or haemorrhagic enteritis
5. Necrotic lesions on the spleen and enlargement of lymph nodes.
Sample collection:
Antemortem materials
1. Nasal, ocular and oral swabs
(best material for diagnosis)
2. Unclotted blood (use EDTA or
Heparin)
3. Lymph node biopsies
Postmortem materials
1. Lymph node
2. Spleen
3. Lungs
4. Intestine:caeum/rectum
5. Ileocaecal junction
Materials for virulent PPR viruses used for challenge experiments are also collected from sacrificed
animals and stored frozen at 20 C.
Another set of tissues should be preserved in 10% neutral buffered formalin for histopathological
examination.
Blood samples collected with anticoagulants (heparin or EDTA) are required for the diagnosis of antigen
by ELISA, nucleic acid detection by RTPCR and for the isolation of virus in cell culture.
Blood samples without anticoagulant should be collected for serological assays. It is advisable to collect
paired serum samples for the seroconversion studies.
Diagnosis:
Laboratory confirmation of the disease is usually done through virus isolation and virus neutralization
assay, which are time consuming and tedious process.
1. Virus isolation remains the gold standard for the diagnosis of PPR. Blood collected at the height of
the temperature is the best material for this purpose. The nasal or ocular swabs or 10% tissue suspension
can also be used.
2. Sandwich ELISA to detect the PPR Antigen
3. monoclonal antibody based competitive ELISA (cELISA) for detection of antibodies against PPRV .
4. Real time RT PCR has been used for quantification and diagnosis of PPR virus. Reverse Transcription
Polymerase Chain Reaction (RTPCR) is the method of choice for detecting nucleic acids of PPRV in
clinical samples.
Control Strategies:
Any Viral disease treatment is not specific hence in case of PPR control measures solely on effective
vaccination to eradicate the disease.
.
In endemic areas, the virus is currently controlled either through the administration of a live attenuated
PPRV vaccine, such as the Nigeria 75/1 strain (Nig 75/1). In India, three live attenuated vaccines are currently
in use: Sungri/96, Arasur/87 and Coimbatore/97 for controlling the disease. Recombinant adenovirus expressing
F and H fusion proteins of PPRV induces both humoral and cellmediated immune responses in goats. Animals
that are vaccinated and those that recover from infection with PPRV generate a long lasting immunity that may
last for a lifetime.
Conclusion :
OIE (Office International des Epizooties) has said ,
in
2011
became
the
first
animal
disease
to
be
eradicated
by
humankind.
The eradication of PPR will have a major positive impact, not only on the livelihoods of poor farmers,
poverty alleviation and food security in pertinent to veterinary public health. OIE has endorsed and stressed
the global control and eradication strategy and launch the global PPR control and eradication campaign, aiming
to eliminate the virus by 2030.
Chapter 2
SAMPLE COLLECTION & SUBMISSION
Fluids are preferable to swabs as the greater sample volume increases the likelihood
of detecting the causal organism. Samples for agent isolation should be placed in sterile
containers. Viruses and many bacteria are susceptible to desiccation especially if collected
on a dry swab. Formulae for suitable transport media for viruses, chlamydia and other
organisms are given in Appendix 2. Whenever possible the sample should be collected
from the specific site of infection. The usual short cotton wool swabs are generally
unsatisfactory for obtaining nasopharyngeal specimens of epithelial cells and mucus for
the investigation of respiratory disease of large animals. Guarded swabs are necessary for
certain bacteriological examinations where misleading results could be generated due to
contaminants from adjacent sites that are colonized with bacterial flora. Similarly, fungal
organisms from the environmentreadily contaminate the nasal passages and upper
trachea. The diagnostic laboratory should be consulted before collecting samples for the
isolation of specific pathogens that require specialized media or culture conditions, for
example, Taylorella equigenitalis, Chlamydophila psittaci or Mycoplasma species. The
laboratory will either supply specialist swabs and transport media or recommend a
reputable source, as appropriate.
SAMPLES FROM SKIN LESIONS
If intact pustules or vesicles are present, the surface should be disinfected with 70%
ethyl alcohol, allowed to dry, and material aspirated from the lesion with a sterile syringe
and fine needle. A swab may be taken from the raw surface of ulcers. A biopsy of wound
tissue should be collected after the superficial area has been cleaned and debrided.
In cases where ringworm is suspected, hair should be plucked from the lesion and
the edge of the lesion scraped with a blunt scalpel blade until blood begins to ooze.Plucked
hair, skin scrapings (including the scalpel blade itself) and any scab material that is
present should be submitted. These specimens will also allow detection of mange or a
bacterial infection, if present. In cases of orf the crust and scrapings from the edge of the
lesion should be collected. In birds feather follicle skin is useful in the diagnosis of Mareks
disease.
BLOOD
COLLECTION OF SPECIMENS
The determination of cause of an infectious disease depends on the isolation and
identification of infectious agent. Four main points should be realized in collecting the
specimens for this purpose :
(i) Most pathogenic bacteria die readily in decomposing tissues.
(ii) Many non-pathogenic intestinal and environmental microorganisms will grow in
decomposing tissues.
(iii) The pathogenic bacteria may be localized in the tissue with lesions or an abscess.
(iv) Allowing samples to dry out kills the bacteria.
It is essential to preserve the pathogens present to prevent contamination, with nonpathogenic organisms and minimize the growth of the contaminants. The means whereby
this can be achieved include collection of samples as early as possible after the death of
animal, working in a dust free environment and keeping the specimens as cool as possible
until it reaches the laboratory.
Use an aseptic technique. Shear the surface of the lesions with a hot scalpel, blade,
slice this surface with sterile blade and swab the cut surface. Never open the alimentary
tract before other organs are sampled. Use swabs only with transport medium. Each
sample should be collected in a separate container. Two or more samples in the same
container leads to cross contamination.
Never freeze the samples meant for bacteriological examination.
Another method of collection of bacteriological specimen is by using Pasteur Pipette.
The sample should be collected from active lesions, old fibrotic lesions are often sterile.
Different kind of specimens listed under each disease should be sent instead of only one
kind of specimen.
8. TUBERCULOSIS : (1) Cough material in sterile container from live animal, (2) Sample
of milk in sterile, container (3) Suspected lesions in 10% Formal-Saline (dead animal), (4)
Smears from lesions fixed by heat and (5) Lymph glands or lung lesions in sterile
container for isolation in 50%buffered glycerine.
9. LEPTOSPIROSIS : (1) Blood, serum (2) Pieces of liver and kidney in 10% formalin (in
dead animals) and (3) Milk or urine in vials by adding 1 drop of formalin per 20ml.
10. SALMONELLA SP. : Intestinal swab, heart blood, bile in sterile container on ice.
11. ACTINOMYCOSIS & ACTINOBACILLOSIS : (1) Smears from pus lesions, pus in
vial on ice (2) Formalin preserved materials from lesions (affected muscle).
12. LISTERIOSIS: (1) Aborted foetus, brain, placenta (2) All internal organs in 10%
formalin/on ice.
13. MYCOPLASMOSIS/CCPP/CBPP/CORYZA: (1) Swab from lesions, nasal and vaginal
swabs in PBS on ice. (2) Piece of lung preserved in 10% formalin for histopathological
examination and on ice separately. Paired serum.
14. CHLAMYDIA/PSITTACOSIS: (1) Nasal swab, lung pieces in sterile container on ice
and internal organs in 10% formalin, (2) Fixed impression smears from liver, lung and
foetus, (3) Paired sera.
15. MYCOTIC INFECTIONS: Deep skin scrap in sterile vials.
16. SKIN DISEASES (RINGWORM, MANAGE, MITES : Skin scrapings for
identification of ectoparasites and fungus in vials.
MATERIAL TO BE COLLECTED FOR VIRAL DISEASES
1. RINDERPEST/RINDERPEST
ALLIED
DISEASE/PPR/BOVINE
VIRUS
DIARRHOEA: (1) From live animals: About 10ml or more blood at the height of
body temp, in anticoagulant, rectal swab in PBS on ice. (2) From dead animals:
Prescapular lymph nodes, spleen, (20-30g) on ice and (3) lung, liver, spleen, tonsil
etc. 10% formalin. Materials 20g from 5 to 6 or more animals be collected and
despatched for better picture of disease/ outbreak. Eye, mouth and rectal swab in
PBS on ice. Pieces of intestine on ice for PPR.
2. FOOT AND MOUTH DISEASE: Vesicular fluid from unruptured oral vesicles
and curetted epithelium from fresh lesions oesopharyngeal fluid in 50% Phosphate
Buffered Glycerine preferably on ice. About 10ml blood at height of body temp. In
EDTA/Heparin, Heart pieces on ice. At least 5 or more animals be investigated and
material collected. Pieces of heart in calves 10% formalin and ice separately.
3. RABIES: Half portion of brain; salivary gland in 50% Phosphate Buffered
Glycerine or Glycerine Saline in water tight hard box and the rest halt portion of
brain in 10% formalin. Alternatively small pieces from Hippocampus and Brain
(Cerebellum, medulla cerebrum, spinal cord) in 50% Buffered Glyerine and 10%
formalin separately duly sealed in bottles and packed in thick polybags and hard
box Labelled Suspected for Rabies:. Where available fresh smears from brain
may be stained with Sellers stain.
4. POX (SHEEP, COW AND BUFFALO): Scab in sterile container on ice, scab in
50% Buffered Glycerine. Skin lesions in 10% formalin, separately.
5. BOVINE HERPES VIRUS 1,2,3/IBR/IPV, BOVINE MAMMILTIS/
PARAINFLUENZA 3/ ADENO VIRUS ETC. Paired serum (sterile) on ice
(IBR/IPV,Bovine Manilitis), Swabs from vaginal and nasal lesions and pieces of
trachea, lung in transport medium on ice., and Smears and pieces of trachea, liver,
turbinate
bone,
lung
in
Bouins
fixative/10%
formalin.
6. SWINE FEVER: Heparinised 20ml blood in sterile vials or test tube on ice form
live animal, Heart blood, pieces of spleen, lymph node, pancreas (10 to 15g each) in
50% Buffered Glyerine Saline, pieces of brain, lung, intestines, ileocaeacal region
and kidney in 10% formalin from dead animal. Material from 5 to 6 or more
animals be collected in order to give diagnosis/true picture of disease. Materials for
isolation and serological tests may be collected in sterile vial on ice without glyerine.
7. BLUETONGUE DISEASE/AFRICAN HORSE SICKNESS/ARBO VERUSES:
Blood at the height of body temperature, in heparin (5-10 units/ml) or EDTA, paired
sera in sterile container on ice. About 10ml blood and 2ml may be collected on ice.
Dead animals-Spleen, lymph nodes (5-6g) on ice Spleen, lymph nodes, intestine,
internal etc. 10% formalin.
8. CAPRINE ARTHRITIS/ENCEPHALITIS/MAEDI/VISNA DISEASE: Paired
serum, joint capsule, lung, brain on ice and 10% formalin.
9. CANINE DISTEMPER: Pieces of lung, urinary bladder, liver, trachea, stomach
wall and brain in 10% formal-Saline, Impression smears from liver, Piece of liver &
spleen in ice.
10. EQUINE INFLUENZA Nasal swab in PBS or Hanks on ice, paired serum.
11. EQUINE INFECTIOUS ANAEMIA (E.I.A): Paired serum, all internal organs in
10%
formalin.
12. INFECTIOUS CANINE HEPATITIS: Liver, gall bladder and kidney in 10%
formalin-Salina. Impression smears from liver fixed in methanol. Spleen and liver in
sterile
containers
on
ice.
13. CANINE PARVOVIRUS: Rectal swab in PBS, pieces of intestines, heart on ice,
all internal organs in 10% formalin.
14. RANIKHET DISEASE: Freshly dead/moribund bird on ice. Portion of liver,
spleen, trachea, bronchi, lung in 50% Buffered Glycerine cerol-Saline in ice and
Proventriculus
in
10%
Formal-Saline.
15. MAREKS DISEASE: Live bird in acute stage of disease Feather follicles from
chest and neck in transport medium Paired serum and Portion of peripheral nerve,
trachea, ovary, liver, kidney, spleen and skin in 10% formalin for histopathology.
16. INFECTIOUS BURSAL DISEASE (GUMBORO DISEASE): Live affected
chick/bird, Bursa of fabricious, kidney, spleen in 10% formalin for histopathology.
17. INFECTIOUS BRONCHITIS/OTHER RESPIRATORY DISEASES: Swab from
exudates, lung Paired sera. (For diagnosis of poultry diseases, it is desired that a few
ailing/moribund/dead birds may be sent for collection of suitable material at the
laboratory)
MATERIAL TO BE COLLECTED FOR PARASITIC DISEASES
1. THEILERIOSIS: Biopsy smears from swollen lymph nodes from early stage of disease
fixed with Methonol. Blood smears fixed in Methanol or alcohol. 2-3 blood smears from
each case.
2. BABESIOSIS: Thin blood smears from early stage of disease fixed with Methanol 2-3
blood smears.
3. ANAPLASMOSIS: Thin blood smears from ear vein fixed with methanol. 2-3 blood
smears.
4. SURRA/TRYPANOSOMIASIS: Blood in anticoagulant on ice, Blood smears fixed.
5. GASTRO-INTESTINAL PARASITIC DISEASES: Faecal sample in 10% formalin IN
dead animals, parasites (round worms in 70% formalin) for identification. All internal
organs in 10% formalin
MATERIAL TO BE COLLECTED FOR TOXICOSES/ POISONINGS
1. AFLATOXICOSIS: Suspected feed (specially groundnut cake) about 100gm. Each,
Piece of liver (50g), spleen in 10% Forma saline and on ice separately.
2. POISONING CASES: Stomach and intestinal contents 100gms. On ice, Left over fodder
100gm and about 100gm liver pieces in alcohol on ice.
3. FORAGE POISONINGS: Sample of grass/fodder, plants, liver and stomach contents on
ice
SAMPLE SUBMISSION
Laboratories usually supply a variety of sample containers and transport media.
The laboratory should supply sample submission forms. These forms must be completed
by the veterinary practitioner and must give specific details of the tests required as well as
clinical history, differential diagnoses, vaccination history, therapy, age of animal, etc. The
latter will enable the microbiologist to choose the most appropriate tests and to avoid
unnecessary expenditure. A complete history will also allow the laboratory to identify a
particularly urgent situation and expedite the processing of the sample. In certain
instances owners may be concerned that information in relation to their animals is kept
confidential. In such circumstances the clinician can assign a code name or number to the
animal and the owner but should never omit clinical detail that will facilitate the selection
of the appropriate tests and the interpretation of the results. The laboratory must be
notified if the differential diagnosis includes a fungal infection or any agent that is
potentially infectious for people.
Many laboratories only set up certain tests on particular days or at a designated
time each day. Clinicians need to familiarize themselves with the laboratory timetable in
order for them to provide an efficient service to their own clients. An awareness of the
time it takes to perform certain assays is essential. Some assays may take weeks to
complete. Where certification is required, samples should be submitted in good time and
allowance should be made for repeat testing and/or the collection of a second sample if
necessary.
The clinician should contact the laboratory in advance if they are not a regular
client or if the tests required are not routine. It is essential to give the laboratory adequate
time to prepare for the receipt of a sample which requires specialist testing. It is
inadvisable, for example, to submit a sample that needs to be passaged on a cell line that
the laboratory does not use on a regular basis without prior discussion. In such
circumstances the sample may have to be stored for days if not weeks, while cells are
resuscitated from the freezer. In certain cases it may be necessary to forward the sample to
a specialist laboratory. If samples are being submitted to a laboratory in another country
an import licence may be required.
Samples should be collected and delivered to the laboratory as early in the day as
possible so that processing can commence on the same day. If a result is required urgently
this must be indicated on the request form and the head of the laboratory should be
notified in advance of the arrival of the sample. Prompt delivery to the laboratory will
maximize the possibility of obtaining a diagnosis. Viruses only replicate in living cells and
a delay in sample submission may result in loss of viability. The transit time needs to be
minimized. Samples should be transported in contact with cold packs or wet ice. If
transportation to the laboratory is delayed, most samples should be held in the
refrigerator at +4C rather than frozen. Serum harvested from clotted blood samples can
be stored frozen for extended periods.
The labelling of samples must be clear and unambiguous. Samples must be
submitted individually in separate leak-proof containers that are clearly marked,
indicating the identity of the sample (tissue, exudate, etc.), animal identification and the
date of collection. Container caps should be screwed on tightly and taped, if necessary, to
avoid leakage. All samples sent in the post must be labelled and packed in accordance with
the regulations of the postal authorities. Glass tubes and other fragile containers must be
adequately packaged to ensure they are not broken in the mail. Samples must always be
surrounded by sufficient absorbent material to soak up the entire sample in the case of
breakage or leakage. In general, triple packaging of diagnostic samples is required. The
secondary or outer packaging must be a rigid container. The package should be clearly
labelled with the words biological substances.
CHAPTER 3
STAINING PROCEDURE
1) LEISHMANS STAIN
This stain may be purchased ready for use or made by dissolving 0.15 g of
Leishmans Powder in 100ml of pure methyl alcohol. The powder is ground in a morter
with a little methyl alcohol, the residue in the morter is treated with more methyl alcohol,
and the process is repeated until all the stain goes into solution. The remaining methyl
alcohol is now added. The stain can be used within an hour or two of making. The stain
consists of methylene blue and eosin. Leishmans stain is suitable for staining blood
smears.
Staining procedure
1. Prepare the smear and dry in air
2. Cover the smear with the undiluted stain and allow to act for one min(usually 1
2 minutes, 3 minutes as per WHO). ( The methyl alcohol in the stain will fix the
film)
3. Add twice the volume of distilled water to the stain on the slide with the aid of a
pipette and mix well and allow staining for 10-12 min. ( The PH of the distilled
water should be neutral. Phosphate buffer ( pH 7.0 ) may be used instead of
distilled water
4. Gently flood the slides with distilled water or buffer and allow the smear
( Usually about 30 sec)
5. Wash thoroughly in running tap water. The stained smear should grossly appear
neither too pink nor too blue, blot off excess water and dry. If the tap water is
highly acidic, resulting in smear turning grossly pink too fast or highly alkaline,
resulting in the smear remaining too blue, try using boiled cooled water or
filtered rain water or pH 6.8 buffered water which can be used as an additional
flooding step after washing in running water.
6. Examine under oil immersion objective of the microscope.
2) GRAMS STAIN
This is most useful and frequently employed stain. Bacteria stained by Grams method, fall in to
Gram Positive and Gram negative groups.
STAINING PROCEDURE.
1.
2.
3.
4.
5.
6.
: Violet
3.GIEMSA STAIN ;
Thin blood films (only) :
1. Fix air-dried film in absolute methanol by dipping the film briefly (two dips) in a Coplin
jar containing absolute methanol.
2. Remove and let air dry.
3. Stain with diluted Giemsa stain (1:20, vol/vol) for 20 min.
For a 1:20 dilution, add 2 ml of stock Giemsa to 40 ml of buffered water in a Coplin jar.
4. Wash by briefly dipping the slide in and out of a Coplin jar of buffered water (one or
two dips). Note: Excessive washing will decolorize the film.
Thick blood films :
1. Allow film to air dry thoroughly for several hours or overnight. Do not dry films in an
incubator or by heat, because this will fix the blood and interfere with the lysing of the
RBCs.
2. DO NOT FIX.
3. Stain with diluted Giemsa stain (1:50, vol/vol) for 50 min. For a 1:50 dilution, add 1
ml of stock Giemsa to 50 ml of buffered water in a Coplin jar.
4. Wash by placing film in buffered water for 3 to 5 min.
5. Let air dry in a vertical position.
: Red
6.SPORE STAIN
Under certain unfavourable circumstances spore forming bacteria (e.g. Bacillus,
Clostridium), develop into spores, which are refractile bodies resistant to various
physical and chemical agents. Shape, size and position of the spore in the bacterial cell are
useful for the identification of sporulated organisms.
REAGENTS
1. Conc. Carbol fuchsin
2. Sulfuric acid 0.5%
3. Loeffles alkaline methylene blue.
STAINING PROCEDURE
1.
2.
3.
4.
5.
INTERPRETATION
The spore will stain red and the vegetative cells will stain blue.
CHAPTER 4
RESULTS
INTERPRETATION OF DIAGNOSTIC RESULTS
There must be full co-operation between the laboratory and the veterinary practice
for the benefit of the patient and owner. It is the responsibility of the clinician to collect
and submit the appropriate samples accompanied by specific requests or adequate history.
It is the responsibility of the microbiologist to interpret the results in relation to the
information supplied. The quality of the diagnostic activity undertaken by the laboratory
depends to a large degree on the clinical and epidemiological information supplied by the
veterinary clinician.
The following points are pertinent when interpreting reports from a diagnostic
microbiology laboratory:
A negative diagnostic report does not necessarily mean that the suspected
microorganism is not the aetiological agent of the condition. There may be many reasons
for the failure of the laboratory to isolate and identify the pathogen, such as a bacterium
being overgrown by contaminants, a virus or other fragile microorganism having died on
the way to the laboratory or the animal may have stopped excreting the microorganisms
before the sample was taken. Unexpected negative results should be discussed with the
head of the laboratory who may decide to perform additional tests for the detection of the
suspect agent. They may also be able to advice on the collection of alternative samples or
suggest other possible causal agents.
It must not always be assumed that the detection of a virus or bacteria establishes
a diagnosis. The laboratory result must be interpreted in light of the clinical signs, history
of vaccination, etc. If the sample is collected from a site normally colonized with bacteria,
including species that have the potential to cause disease in certain circumstances, the
culture results need to be interpreted cautiously. Some latent viruses may reactivate and
bacteria proliferate in response to a condition which they have not caused. Apparently
healthy animals can be subclinical shedders of microorganisms such as salmonellae,
rotaviruses, enteroviruses or coronaviruses in faeces and leptospires in urine. Even if these
potential pathogens are isolated and identified, the illness or death might have been due to
another cause.
The clinician should always discuss unexpected results with the laboratory. This
may assist the head of the laboratory in the early detection of a problem with a particular
technique. The microbiologist must be prepared at all times to act on information received
and to critically appraise the procedures in the laboratory.
It is important that the difficulties associated with some types of samples and the
limitations of certain assays are flagged to the clinician. This will help to promote
intelligent utilization of the laboratory and to avoid unrealistic expectations.
Bacteria such as members of the Enterobacteriaceae are ubiquitous. Isolation of
microorganisms may represent contamination of the sample by faeces or soil or their
presence could be due to post-mortem invasion.
Some bacteria such as salmonellae, leptospires or Mycobacterium avium subsp.
paratuberculosis may be shed intermittently. A repeat sample following a negative
examination report might be worthwhile.
Escherichia coli in diarrhoeal faecal samples from farm animals is usually only
significant if the animal is under 10 days of age and if the E. coli isolate possesses the K88,
K99, F41 or 987P fimbrial antigens, associated with enteropathogenicity. Pigs are the
exception as they are also susceptible to colibacillosis soon after weaning and to oedema
disease in the growing period.
Diagnosis of a mycotic disease thought to be due to a ubiquitous fungus such as
Aspergillus fumigates should always be confirmed histopathologically. It is necessary to
demonstrate the fungal hyphae actually invading the tissue and causing a tissue reaction.
Herd or flock vaccination programmes will modify the interpretation of serological
tests. Serological diagnosis of recent viral exposure usually requires the comparison of two
samples collected approximately two weeks apart.
Conflicting results may reflect the sensitivity of different assays. Thus a sample
that is negative by enzyme-linked immunosorbent assay (ELISA) and by isolation may be
positive by PCR.
CHAPTER 5
ANALYSIS OF MILK SAMPLES :
A)Detection of Mastitis :
1. Bromothymol blue test: The reaction of milk is detected by this test. In this test, 1 ml
of 0.2% bromothymol blue solution is taken and to this 5 ml of freshly collected milk
sample is added. If sample is alkaline due to mastitis, it becomes blue to green.
However, the test may give false positive reaction in later stage of lactation.
2. Chloride test: The presence of more than 0.14% chloride content in milk is
considered abnormal. For this test, take 5 ml of 0.1341% silver nitrate solution in a
test tube and add 2 drops of 10% potassium dichromate solution as indicator. To
this add 1 ml of milk sample and mix it properly. If the chloride is less than 0.14%
then it will remain brownish red while in positive case it will become yellow
indicating more than 0.14% chloride content in milk. However, this test may give
false positive reaction during early and late lactation.
3. White slide test: In this test, 4-5 drops of test milk sample are placed on a clean dry
glass slide. To this add a drop of 4% sodium hydroxide and mix with a glass rod. If
the milk is from animal having mastitis, it becomes thickened and flakes.
4. California mastitis test: This test is based on increased number of leukocytes and
increased alkalinity in milk due to mastitis. Take 0.5 ml milk from each quarter in
plastic peddle cups and add equal quantity of mastoid solution and mix well by
circular movement of peddle on a horizontal plane.
The results are read as follows:
a. Liquid milk with no streaks or precipitation: Negative for mastitis.
b. Streaky fluid: + (weak positive cell count 500,000/ml).
c. Slimy:
++
d. Gelatinous:
+++
This is an indirect measurement of cell count.
TEST PROCEDURE
1. Measure out 2 ml of milk in a test tube.
2. Add 2 drops of antigen using 1 ml pipette.
3. Shake the mixture by rotating between palms taking care not to shake so vigorously as to cause it
to froth. Mixing should be done within 1 min after addition of antigen. The appearance of pink
colour in the top layer of the milk column after allowing to stand for a few min indicates that
mixing has been incomplete. In such a case, mix again thoroughly.
4. Incubate at 37 c for 1 hr
5. After incubation allow the tubes to stand for about 90 min at room temperature. There is no shift
in reaction even when the tubes are left standing several hrs.
6. Interpretation
7.
An intensely cherry coloured ring at the cream layer and absence of
colour in the milk below the cream layer
+++
++
Negative
PRESENTATION
The antigen is available in a minimum packing at 20 ml
TRANSPORTATION
Either by post or through messenger.
CHAPTER 6
DIAGNOSIS OF POISONINGS IN ANIMALS
The veterinarians should collect the proper samples from the suspected cases of
toxicosis and forward them to the appropriate laboratory for confirmation and diagnosis
after proper labelling. The veterinarian may also carry out certain simple tests at his own
level in order to provide a rapid diagnosis and initiate the treatment. For this purpose,
some of the simple diagnostic tests are described here, so that these can be performed in a
clinical pathology laboratory or disease investigation laboratory with minimal facilities.
CYANIDE POISONING
1.
a. Take 1 ml supernatant of centrifuged gastrointestinal contents in a test tube. Add
2 ml of 10% sodium hydroxide solution.
b. Add 2 ml of 10% ferrous sulfate solution (freshly prepared in distilled water).
c. Add 10% hydrochloric acid in sufficient quantity, which can dissolve the
precipitate formed in the mixture due to ferrous oxide formation. Blue colour will develop
due to formation of ferriferrocyanide complex (Prussian blue) in the test tube if cyanide is
present in the sample of gastrointestinal contents.
2.
a. In a flask, take 5.0 gm sodium carbonate and 0.5 gm picric acid; dissolve in 100
ml distilled water. The filter paper strips (1 cm wide x 5 cm long) are wet in the solution
and dried in air.
b. Take about 5.0 gm of moist plant or rumen content and grind into small pieces
and keep in test tube. Add few drops of chloroform.
c. The strip is placed at the mouth of test tube, 2/3 part of which should be in test
tube. Apply stop cork on the test tube. Incubate for 24 hours at 37C or keep in a warm
place.
d. In cyanide positive cases, the filter paper strip becomes reddish brown in colour.
NITRATE POISONING
FOR PLANTS
1.
a. In a 100 ml volumetric flask, take 20 ml distilled water and dissolve 0.5 gm
diphenylamine. Add sufficient quantity of sulfuric acid to make it 100 ml. Store the
solution in a dark coloured bottle.
b. For testing of nitrate in plants, put one drop of above solution on the cut surface
of plant; in positive cases, the cut surface of plant will become green or blue in colour.
FOR FEED AND FODDER
2.
a. It is applied on prepared and grinded fodder and feed. In this test, take about 5
gm ground feed and mix it with 5-10 ml distilled water in a beaker, mix well and keep for
15-30 minute.
b. Centrifuge the contents and take out 2.0 ml supernatant in a test tube and add 2
ml of 0.3% sulfanilic acid prepared in 20% glacial acetic acid, mix well.
c. Add 2 ml of 0.15% alphanaphthylamine hydrochloride in 20% glacial acetic acid.
Development of a pink to red colour is an indication of the presence of nitrate in the
sample.
FOR STOMACH, INTESTINAL CONTENTS AND URINE
3.
a. This type of test can be applied to stomach or intestinal contents and in
urine.
b. In a test tube take 0.1 ml of the clear stomach contents after centrifugation or
urine.
c. Add 0.1 ml sulfanilic acid reagent and mix well.
d. Add 0.1 ml imipramine solution and 0.2 ml concentrated sulfuric acid and mix
thoroughly.
e. Development of a blue colour confirms the presence of nitrate in the sample.
NITRITE POISONING
1. Take 1 ml clear stomach contents or urine in a test tube and add 0.1 ml imipramine
solution. Mix well.
2. Add 0.2 ml hydrochloric acid.
3. The development of a blue colour in the tube is indicative of nitrite in the sample.
OXALATE POISONING
1. In a test tube, take 1 ml clear stomach contents or urine and add 0.2 ml concentrated
ammonium hydroxide and heat it on flame of a burner or spirit lamp to evaporate the
solution.
2. Add 40 mg thiobarbituric acid crystals and gently reheat on burner or spirit lamp.
3. Formation of an orange red colour, which is soluble in ethanol is indicative of
oxalates in the samples.
STRYCHNINE POISONING
1. Strychnine can be detected in stomach contents, intestinal contents, serum and urine.
2. In a clean and dry petridish, take 1-2 ml clear stomach contents or urine and serum.
Add equal quantity of concentrated sulphuric acid and mix well with the glass rod.
3. Add few crystals of potassium dichromate in the contents of petridish. Mix the contents.
4. Examine the plate for change of colour for 5-10 minute. A change in colour from yellow
orange to greenish blue is indicative of the presence of strychnine.
ARSENIC POISONING
1. Take 1 ml of the stomach content fluid in a test tube. Add small crystal of zinc and 2-3
ml hydrochloric acid and 0.5 ml iodine (Lugols iodine).
2. Place an absorbent cotton plug and put a filter paper strip wet in silver nitrate solution.
(Note: In place of silver nitrate strip, one can also use filter paper strip impregnated in
mercuric chloride.)
3. If arsenic is present in the sample, the filter paper becomes yellow which may turn
black if water is applied on it.
LEAD POISONING
CHAPTER 7
VETERO LEGAL CASES
VETERINARY FORENSIC MEDICINE AND SPECIMEN COLLECTION
AN OVERVIEW
Veterinary Forensic Medicine is one of the rapidly developing branches of Veterinary Science. It
has become the attention gaining subject now-a-days as it deals with the application of veterinary
knowledge to aid in the administration of justice.
Vetero-legal medicine, verinary Forensic medicine and Veterinary Jurisprodence are synonymously
used. Veterolegal medicine insolves, as the name implies, the veterinary expertise with law matters. In
verolegal cases a veterinarian is called an expert witness and he/she must have adequate knowledge on
veterinary subjects and legal procedures and animal related acts. Vetero legal medicine involves
postmortem examination, using health, wound or soundless, protecting animal rights, investigation of
offences against animals, poisonings and fraudulence of animals or animal products.
Vetero legal specimen collection for the laborotory investigation of animal diseases or poisoning is
the essential step. Specimens may be collected from live animals, dead animals or from environment for
the variety of purposes. These specimens should in correct quantity and quality to provide valid results.
After sampling, they are carefully packed, labelled and transported to laboratory as quickly as possible
with adequate requirements.
The main part of veterolegal medicine is postmortem examination.
CONSIDERATION FOR POSTMORTEM EXAMINATION IN LEGAL CASES
. A vetero legal postmortem examination should be done by written order from the Police officer
or the Executive Magistrate (RDO is subdivisional executive magistrate-I and Thasildar is
subdivisional executive magistrate-II).
. Refer examination, carefully read police report (Ask for History of the Case or FIR xerox).
.l The examination should be done in day light (Official court permitted timing : 10.00 am to 5.00
pm)
. No unauthorised person should be allowed to be pressent at the time of postmortem examination.
The investigating polce officer may however be present.
SPECIMEN COLLECTION
The starting point of laboratory investigation of an animal disease or vetero legal case is the specimen
collection. Samples may be taken from live or dead animals or from environment. The purpose of sample
collection is to diagnose a disease, or for disease survillance, or for detecting the poisoning, if any, or for health
certifying or to monitor the response to vaccination or treatment. Equally the packaging, labelling and transport
of sample is also important.
COLLECTION, PACKING AND DELIVERY OF SAMPLES FOR TOXICOLOGICAL ANALYSIS
.
Organic materials / viscera to be collected depend upon the nature of toxic chemical suspected. In the
absence of this knowledge, specimens such as stomach and rumen with its contents, small intestinal loop with
its contents, liver bit, kndney bit, brain bit, blood, urine, vomits (if present) may be particularly useful for
toxicological analysis.
500 gms
Kidney
One half
Brain
500 gms
200 gms
Hair
5 to 10 g
Intestinal Content
580 gms
Stomach Content
Whole Blood
30 ml
Serum
15 mL
Urine
50 ml
Faeces
10 gm or Faecal swabs
Collect each isceral specimen in a separate wide mouthed glass bottle/plastic bottle liver and kidney may be
combinedly collected in a single bottle.
Add preservative (Rectified spirit (or) saturated Sodium chloride (common salt) solution and tightly closed
it.
Seal the viscera box with red sealing wax and paste a label on the top of box also.
Hand over te viscera box to the concerned Head Constable who came for P.M. with a letter of request, copy
of the label and Form No.66 (refer appendix II)
Submit the Samples to Regional Forensic Lab, Animal Toxicology Division Situated in Salem / Tanjore.
PRESERVATIVES USED
I. Visceral Samples
II. Blood
III.Faeces
Not needed
IV.Urine
V. Milk
Vi.Feed, samples from environment, water swabs from feeders and ventilation duct. - Prervative not needed.
SUSPECTED
POISION
Arsenic
POSSIBLE SOURCES
Weed killers, Rat poisons, Fly
papers, Wall papers, Sheep-dip,
Artifical Flowers, As mordant in
dyeing
SPECIMENS REQUIRED
Acute poisoning cases :
Stomach, Intestine, Liver, Kidney, Blood,
Urine and feed.
Chromic poisoning casess :
Stomach, Intestine, Liver, Kidney, Blood,
Urine, Feed, Hair and Bone.
2.
Antimony
Medicaments
3.
Alkaloids
Toxic Plants
4.
Ammonia
Urea
Parasiticidal preparation
(Urea Poisoning)
5.
Copper
Agricultural fungicides
6.
Cyanide
Feed (Sorghum)
(HCN)
7.
Organo Chloride
Substances
8.
Organophoshorus
Components
9.
Lead
Mercury
11.
Nitrates
(Quick Transport is
required as they
disintegrate rapidly)
12.
Phosphorus
13.
Oxalates
14.
Rat Poisons
Rodenticides
Date
Dr.Veterinary Hosp.
Time of Exam..Place of Exam
This is to certify that at the request of (1) ..........
Vide his/ their letter/reference No..date
I have on this the day of.examined
..Having the following identification marks.........
(kind of animal)
.
.
belonging to (Name and address of the owner of the animal)
..
The said animal has got the following injuries on its body.
..
..
Opinion as to the cause of injuries..
Designation..
Date.. .
V.C.reg. No.
2. CERTIFICATE OF SOUNDNESS
No.
Date:
I have this day examined at the request of Shri -----------------------------------------------------------------------------S/o ------------------------------------------------------------Address-------------------------------------------------------------------------------------------Kind of Animal-----------------------------------------------------------------------------------Breed of Animal---------------------------------------------------------------------------------Age-----------------------------------------Colour---------------------------Height------------Identification Marks-----------------------------------------------------------------------------
Lactation-------------------------------------Bands---------------------------------------------In my opinion the above animal is ---------------------------------------------------------------------------------------------------------------------------------------------------------------Signature & Designation of issuing officer
Registration No
Species--------------------------------Sex ----------------- Age---------------------Breed -----------------------------Colour---------------------------------------------Identificaton marks-------------------------------------------------------------------I, the undersigned, do hereby certify thatI am the owner (duly authorized
agent for the owner) of the above mentioned animal, that I do hereby give the
veterinarian/his representative --------------------------------------------------full and
complete authority to destroy the said animal in whatever manner the said
CHAPTER 8
ANIMAL DISEASE SURVEILLANCE INFORMATION SYSTEM (ADSIS)
Animal Disease Surveillance Information System (ADSIS) is a system to know quickly the status
of animal diseases in a region for taking appropriate action. The objectives of the system are twofold:
(i) Prevention, Control and Eradication of diseases by sharing information with others and using
the latest techniques in diagnosis and use of vaccines.
(ii) Promotion of international trade in livestock and livestock products.
SURVEILLANCE :
Surveillance is keeping a constant vigil on the occurrence of diseases; it is an active disease
accounting process and an essential prerequisite in prevention, control and eradication of diseases. It has
three components :
(i)
Collection of data
(ii) Analysis
(iii) Interpretation and prompt dissemination of disease intelligence information.
DISEASES FOR SURVEILLANCE :
Disease for surveillance falls under two categories :
Notification by telex / telephones / fax / special messenger within 24 hours of first occurrence
or reoccurrence of list A diseases in the region.
(ii) Weekly reports subsequent to notification to provide further information on the evolutionof
the incident. These reports should continue until the disease stabilises / subsides and monthly
reporting as per item (iii) below will suffice the needs thereafter.
(iii) Monthly report on the presence or absence of diseases in list A.
(iv) Annual report on the presence or absence of all diseases in list A, B and C and others
considered to be of socio-economic importance or of major veterinary interest.
For the purpose of this State Administration the diseases to be reported under item i, ii and iii
above will be,
List A : All diseases
List B : Avian infectious bronchitis (B301); Anthrax (B051); Haemorrhagic Septicaemia (B109);
Infectious bursal disease (B309); Mareks disease (B310), Theileriasis (B111).
List C : Black leg or Black quarters (C614); Enterotoxemia (C704)
Nil Report
In case there are no diseases to report use the Nil Report Proforma (Pdl.No.3)
The proforma are so designed that writing is minimal. Alfa and numerical codes are used for
which you refer the proforma and read carefully. Codes for the blocks and districts are given in
Appendix III. Wherever names occur use capital letters. Avoid over writing and make things legible for
the person who used NIC to transmit the message to the computer without ambiguity.
QUALITY REPORTING :
Quality reporting is of paramount importance for disease intelligence work. For this purpose
there must be a common understanding of the meaning of certain terminologies used in disease reporting.
Given below are definitions of some terms based on the OIE publications :
i) International Animal Health Code
ii) Manual of standards for diagnostic and vaccines.
INCIDENCE :
Incidence means the number of new cases of outbreaks within a specified period of time in a
defined animal population.
e.g. Incidence of Rinderpest in Tamilnadu
CASE :
An individual animal affected by one of the infectious or parasitic diseases being made clear
through clinical signs / laboratory diagnosis. eg., It was a case of Rinderpest based on clinical diagnosis.
INFECTED ZONE :
A clearly defined territory in which a disease has been diagnosed. This are must be clearly defined
and degreed by the Veterinary Administration. For this State this zone occupies a radius of 8 kilo meters
around the foci of the incidence of the disease for Rinderpest and Foot and Mouth (Specify for other
diseases)
The length of time during which the infected zone is maintained will vary according to the diseases
and sanitary and control methods adopted.
In case of Rinderpest the infected zone shall be considered as such until at least 21 days
(Incubation period of Rinderpest) have elapsed after the last case has been reported and following the
completion of a stamping out policy and disinfection procedures or six months after the clinical recovery
or death of the last affected animal if the stamping out policy is not adopted.
In case of Foot and Mouth infected zone is a zone having a depth of at least 10 kilometer around
the foci of Foot and Mouth disease. It is seperated from the remainder of the country a surveillance zone
with a depth of at least 10 kilo-meters. In both zones animal movement must be controlled.
To regain the disease free status two years should lapse after the last case has recovered without
stamping out policy.
OUTBREAK OF DISEASE :
Outbreak of disease means an occurence of one of the diseases in List, A, B or C in an agricultural
establishment, breeding establisment or premises, including all buildings and all adjoining premises,
where animal are present.
Where it cannot be defined in this way, the outbreak shall be considered as occuring in the part of
the territory in which, taking local conditions into account, it cannot be guaranteed that both susceptible
and non-susceptible animals have had no direct contact with affected or suspected cases in that area.
For example, in the case of certain parts of Africa, an outbreak means the occurence of the disease
within a sixteenth square degree; the occurence is still referred to as an outbreak even though the disease
may occur in several places within the same sixteenth square degree.
STAMPING-OUT POLICY :
Stamping-out Policy means the carrying out under the authority of the Veterinary Administration
on confirmation of a disease, of animal health prophylactic measures, consisting of killing / destroying the
animals which are affected and those suspected of being affected in theherd and when appropriate, those
in other herds which have been exposed to infection by direct animal to animal contact, or by indirect
contact of a kind likely to cause the transmission of the causal pathogen. All susceptible animals,
vaccinated or unvaccinated on an infected premises should be killed and the carcasses destroyed by
burning or burial, or by any other method which will eliminated the spread of infection through the
carcasses or products of the animals killed. This policy should be accompanied by the cleaning and
disinfection procedures as defined in the code. The term modified stamping-out policy should be used
in communications to the OIE whenever the above animal health measures are not implemented in full
and details of the modifications should be given.
STABILISATION OF DISEASE :
In an outbreak, the disease is considered stabilised in the nth week only when the affected animal
population in the nth week is less than those in (n-1)th week is observed, however for the nth week a
weekly report should be sent indicating Disease Stabilised on it.
MANDATE :
The following are the mandates to the Veterinarians at various levels.
VETERINARIAN (INCHARGE) OF THE REPORTING UNITS :
Animal disease surveillance is one of the important duties of the Veterinary Surgeon and he should
keep the following :
i)
ii) A professional register where in the incidence of the diseases in OIE list A, B and C will be
recorded with details for annual reporting to the Veterinary Administration.
iii) Livestock census of the Jurisdiction updated every first at April of the year.
iv) Statistical bulletin of the Animal Husbandry Department which lists theaverage values of
livestock and livestock products.
v) List of livestock and poultry farms in the jurisdiction in the private and co-operative sectors
and under educational institutions including the University.
vi) Veterinarians in private practice and under Non-Governmental Institutions.
He should organis the Clinical Laboratory and examine clinical materials at least from 5-10% of
clinical cases attending the dispensary / Hospital.
ASSISTANT DIRECTOR OF ANIMAL HUSBANDRY :
Soon after the receipt of FIR the Assistant Director will organise the various steps to contain the
disease such as deployment of the Disease Investigation Unit/Disease Intelligence Unit and the vaccination
squad in the area so that the Veterinarian incharge of the Dispensary is not much disturbed from his
treatment work.
JOINT DIRECTOR OF ANIMAL HUSBANDRY :
The Joint Director will be responsible for the prompt entry of data in the computer net work at
the NIC and do such things for electronic mailing of the output reports to the concerned authorities. He
will supervise the operations and give directions to the staff for quick containment of the disease.
He will keep ready always a contingency plan in respect of control of outbreaks.
S. No.
DISEASE CODE
DISEASE NAME
1.
A010
2.
A011
FMD - VIRUS O
3.
A012
FMD - VIRUS A
4.
A013
FMD - VIRUS C
A014
6.
A015
7.
A016
8.
A017
9.
A018
10.
A020
11.
A021
VS - VIRUS INDIANA
12.
A022
13.
A023
14.
A030
15.
A040
RINDERPEST
16.
A050
17.
A060
CONTAGIOUS BOVINE
PLEUROPNEUMONIA
18.
A070
19.
A080
20.
A090
BLUETONGUE
21.
A100
22.
A110
23.
A120
24.
A130
HOG CHOLERA
25.
A140
TESCHEN DISEASE
26.
A150
FOWL PLAGUE
27.
A160
28.
B051
ANTHRAX
29.
B052
AUJESZKYS DISEASE
30.
B053
ECHINOCOCCOSIS / HYDATIDOSIS
31.
B055
HEARTWATER
32.
B056
LEPTOSPIROSIS
33.
B057
Q FEVER
34.
B058
RABIES
35.
B059
PARATUBERCULOSIS
36.
B060
SCREWWORM (COCHLIOMYIA
HOMINIVORAX)
37.
B101
ANAPLASMOSIS
38.
B102
BABESIOSIS
39.
B103
40.
B104
41.
B105
BOVINE TUBERCULOSIS
(MYCOBACTERIUM BOVIS)
42.
B106
CYSTICERCOSIS (C.BOVIS)
43.
B107
DERMATOPHILOSIS
44.
B108
45.
B109
HAEMORRHAGIC SPETICAEMIA
46.
B110
47.
B111
THEILERIASIS
48.
B112
TRICHOMONIASIS
49.
B113
TRYPANOSOMIASIS
50.
B114
S. No.
DISEASE CODE
DISEASE NAME
51.
B115
BOVINE
SPONGIFORME
ENCEPHALOPATHY (BSE)
52.
B151
53.
B152
54.
B153
55.
B154
CONTAGIOUS AGALACTIA
56.
B155
CONTAGIOUS
PLEUROPNEUMONIA
57.
B156
58.
B157
PULMONARY ADENOMATOSIS
59.
B158
60.
B159
SALMONELLOSIS
61.
B160
SCRAPIE
62.
B161
MAEDI - VISNA
63.
B201
64.
B202
DOURINE
65.
B203
EPIZOOTIC LYMPHANGITIS
66.
B204
EQUINE ENCEPHALOMYELITIS
67.
B205
68.
B206
69.
B207
70.
B208
EQUINE RHINOPNEUMONITIS
71.
B209
GLANDERS
CAPRINE
72.
B210
HORSE POX
73.
B211
74.
B212
JAPANESE ENCEPHALITIS
75.
B213
HORSE MANGE
76.
B214
77.
B215
SURRA (T.EVANSI)
78.
B216
VENEQUELAN
ENCEPHALOMYELITIS
79.
B251
ATROPHIC RHINITIS
80.
B252
CYSTICERCOSIS (C.CELLULOSAE)
81.
B253
82.
B254
TRANSMISSIBLE GASTROENTERITIS OF
PIGS
83.
B255
TRICHINELLOSIS
84.
B301
85.
B302
AVIAN
LARYNGOTRACHEITIS
86.
B303
AVIAN TUBERCULOSIS
87.
B304
DUCK HEPATITIS
88.
B305
89.
B306
FOWL CHOLERA
90.
B306
FOWL POX
91.
B308
92.
B309
INFECTIOUS
BURSAL
(GUMBORO DISEASE)
93.
B310
MAREKS DISEASE
94.
B311
MYCOPLASMOSIS (M.GALLISEPTICUM)
95.
B312
96.
B313
97.
B351
MYXOMATOSIS
98.
B352
TULARAEMIA
99.
B353
100,
B401
EQUINE
INFECTIOUS
DISEASE
101.
B404
102.
B405
INFECTIOUS
NECROSIS
103.
B406
HERPESVIROSIS OF SALMONIDS
104.
B408
RENIBACTERIOSIS
105.
B411
HERPESVIROSIS OF ICTALURIDS
106.
B413
EPIZOOTIC
NECROSIS
107.
B414
EDWARDSIELLOSIS (E.ICTALURI)
108.
B431
BONAMIOSIS
109.
B432
HAPLOSPORIDIOSIS
110.
B433
PERKINSOSIS
111.
B434
MARTEILIOSIS
112.
B435
LRIDOVIROSIS
113.
B451
ACARIASIS OF BEES
114.
B452
115.
B453
116.
B454
NOSEMOSIS OF BEES
117.
B455
VARROASIS
118.
B501
LEISHMANIASIS
119.
C611
LISTERIOSIS
120.
C612
TOXOPLASMOSIS
121.
C613
MELIODOSIS
122.
C614
BLACKLEG
123.
C615
BOTULISM
124.
C616
125.
C617
OTHER PASTEURELLOSES
126.
C618
ACTINOMYCOSIS
127.
C619
128.
C620
COCCIDIOSIS
129.
C621
130.
C622
FILARIASIS
HAEMATOPOIETIC
HAEMATOPOIETIC
131.
C652
132.
C653
VIBRONIC DYSENTERY
133.
C654
WARBLE INFESTATION
134.
C701
135.
C702
FOOT-ROT
136.
C703
CONTAGIOUS OPHTHALMIA
137.
C704
ENTEROTOXAEMIA
138.
C705
CASEOUS LYMPYHADENITIS
139.
C706
SHEEP MANGE
140.
C751
141.
C752
ULCERATIVE LYMPHANGITIS
142.
C753
STRANGLES
143.
C801
SWINE ERYSIPELAS
144.
C851
INFECTIOUS CORYZA
145.
C853
AVIAN ENCEPHALOMYELITIS
146.
C854
AVIAN SPIROCHAETOSIS
147.
C855
AVIAN SALMONELLOSIS
148.
C856
AVIAN LEUCOSIS
149.
C921
CANINE DISTEMPER
1.
01
BOVINE
2.
02
OVINE
3.
03
CAPRINE
4.
04
SWINE
5.
05
CAMEL
6.
06
EQUINE
7.
07
CANINE
8.
08
FELINE
9.
09
WILD FAUNA
10.
10
AVIAN
: 006
Name Of Block
1. Bhuvanagiri
2. Keerapalayam
3. Kattmannarkoil
4. Parangipettai
5. Panrutti
6. Cuddalore
7. Kurinjipadi
8. Kumaratchi
9. Annagramam
10.
Viruthachalam
11.
Nallur
12.
Mangalur
13.
Kammapuram
Code
: 0310
: 0130
: 0350
: 0320
: 0240
: 0260
: 0250
: 0340
: 0230
: 0280
: 0270
: 0300
: 0290
Designation
District
Institution
Institution Code
Registration No
A. Animal Disease and its Initial Occurrence :
A.1. Disease Name ____________________________________________________________
A.2. Disease Code
A.3. Date of Initial Occurrence (dd/mm/yy)
B. Location Particulars
B. 1. District Code & Name
B.2.
B.3.
Municipality / Corporation
B.4.
Village Name______________________________________________________________
C.
Date :
Details of Outbreak
C.1
Species Code
C.2
Number Affected
C.3
Number of Deaths
Signature
Name
Designation
:
:
:
1. Disease
a.
b.
c.
d.
Name
OIE Code
Registration No.
Disease Status
a.
b.
New
Old + New
a.
b.
c.
a.
b.
c.
d.
e.
f.
g.
a.
b.
c.
d.
e.
f.
a.
b.
c.
d.
a.
b.
c.
d.
a.
b.
c.
d.
Clinical Symptoms
Lab. Confirmation
PM Confirmation
Segregation
Vaccination
Ring Vaccination
Stamping
Burial
Incineration
Disinfection
Breeding Stock
Fattening Stock
Individual Herds
Collective Herds
Stall Fed Population
Grazing Population
Introduction Mode
Exotic
Indigenous
Cross
Viral
Bacterial
Parasite
Others
Serological
Biological
Isolation
Allergic
2. Village Affected
3. Number of Outbreaks
4. Species Affected
5. Nature of Diagnosis
7. Affected Population
9. Causal Agent
10. Laboratory
Confirmation
11.
Manifestation of Malady
12.
Infected Zone
13.
Uninfected Zone
No. of Villages
No Vaccination
No Treated
No Vaccinated
a. Milk Quantity
b. Milk Value
c. Meat Quantity
d. Meat Value
e. Egg Quantity
f. Egg Value
g. Wool Quantity
h. Wool Value
i. Days in Nos.
ii. Value Rs.
Kg.
Rs.
Kg.
Rs.
Kg.
Rs.
Kg.
Rs
4. Species Codes
BOV - Bovine
CAM - Camel
CAN - Canine
CAP - Caprine
OVI - Ovine
SUI - Swine
EQI - Equine
FEL - Feline
8 a. Introduction Mode
D - Direct
I - Indirect
B - Both
Y
O
U
N
G
A
D
U
L
T
Y
O
U
N
G
A
D
U
L
T
Y
O
U
N
G
A
D
U
L
T
Y
O
U
N
G
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Exotic
Indigenous
A
D
U
L
T
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Affected
(No)
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Date :
Species Affected :
Dead
(No)
Appr. Value
of Dead
Animals
(Rs.)
Registration No.:
Destroy
ed (No)
Appr. Value
of Destroyed
Animals(Rs.)
Slaugh
tered
( No)
Appr. Value
of
Slaughtered
Animals
(Rs.)
Vaccinated
(No.)
Disease :
Species Affected :
Registration No.:
Disease :
Species Affected :
Registration No.:
Disease :
Species Affected :
Registration No.:
Crossbred
Exotic
Indigenous
Crossbred
Exotic
Indigenous
Crossbred
Signature
Treat
ed
(No)
Susceptib
le (No)
Designation
District
Institution
Date :
Signature
Name of
Sl.
No.
Name
Name
of the
of the
Disease
Village
Name of
Name
Last
the
of the
occurrence
Anticipated
Panchayat
Distric
of the
occurrence
Union
disease
the
Institutio
n
Vaccine
indent
month
Vaccination
Month
Name of
Sl.
No.
Name of
the
Disease
Name of
the
the Village
Name of the
District
Institution
Livestock
Population
No.of
Vaccination
of the Village
done
Date of
Vaccination
% of
Vaccination
From
To
The Assistant Director,
Animal Disease Intelligence Unit,
Cuddalore
R.O.C.No
date
Sir,
Sub : Specimens for examinations - reg.
The following samples may please be examined and the results be sent at the earliest.
Sl. No.
Sample No.
Case No.
Species
Specimen
Date of
Collection
Disease
Symptom
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Yours faithfully,
Expected
CHAPTER 9
HELMINTHOLOGY
INTRODUCTION
Diagnosis of Endoparasitic Infection to combat anthelmintic resistance:
Helminthiasis caused by nematodes, trematodes and cestodes is the most
important cause of lost production in small and large ruminants in many parts of the
world. In developing countries like India too face these problems of parasitic infection
more than developed countries since its a land of agriculture and animal husbandry
activities. This infection causes myriad of problems in the health of livestock causing
burdens to livestock owners and eventually to the countrys GDP. Apart from infection
by parasite nowadays helminthic resistance is also becoming major concern for
veterinary practitioner in their practice when drugs flunk to eliminate the infection.
When livestock producers use anthelmintic parasite-control products in their herd and
fail to see a response, there are a number of factors to consider. Was the timing of use
appropriate to minimize re-infection? Did the dosage match the weight of the animals?
Or did the product fail to achieve a response because the parasite population has
become resistant to the dewormer of choice? Even though different factors contribute to
the resistance, the most pressing reasons are low dosage and indiscriminate
administration of anthelmintic by livestock owners and quacks. Anthelmintic resistance
is already a serious problem in some parts of the world, not just in sheep and goats but
also in cattle. Drug resistance was first defined in nematodes by Prichard et al. in 1980.
The earliest reports of anthelmintic resistance in sheep involved Benzimidazoles,
followed by resistance to levamisole and finally resistance to macrocylic lactones like
ivermectin. Resistance has been reported from all the four corners of the world, to all
available drugs, in all classes of helminthes.
Veterinarian role in combating these problems will enhance the
livelihood of farmers and also will be fruitful for the future generation of veterinary
practitioner. Even though many factors contribute to this resistance, as a veterinarian
how far we can minimize further resistance. For example Cyathostome, a small
strongyle in horse resistance to fenbendazole, pyrantel pamoate , oxifendazole and
showing sensitivity to ivermectin Kaplan et al (2003). There are only three broadspectrum anthelmintics available for the control of helminths: group 1, the
benzimidazoles
flubendazole,
(albendazole,
mebendazole,
cambendazole,
oxibendazole,
ciclobendazole,
ricobendazole,
fenbendazole,
thiabendazole
and
- Michael
Ref: Lalchhandama (2010), Anthelmintic resistance: the song remains the same.
BZs = benzimidazoles; IZs = imidazothiazoles [M = morantel, P = pyrantel]; MLs =
macrocyclic lactones [IVM = ivermectin, MXD = moxidectin, DRM = doramectin]; SNs
= salicylanilide [MBC = milbemycin; CST = closantel]; RXN = rafoxanide; OPP =
organophosphate; OXA = oxamniquine; PPZ = piperazine.
Fecal samples where the amount of feces obtained is too small to handle with any
other technique.
As an adjunct to a flotation technique where you are looking for eggs that do not
float. (In this case you probably would be better off running ethyl acetate
sedimentation and examining the resultant pellet using the direct smear method.)
A small amount of feces (a pea to grape size, about 1 gram) is mixed with about
10 ml of flotation medium and poured into a tube so that the liquid comes just
over the top of the tube. The mixture is allowed to sit for about 15 min while the
eggs float to the top and the rest of the fecal matter sinks to the bottom. A cover
slip can be placed on the top of the tube before the incubation period starts or
can be applied at the end. The cover slip is then transferred to a microscope
slide.
Commercial devices have a sieve incorporated into the tube to keep large
particles from floating up and sticking to the coverslip. See image below of some
commonly available commercial fecal devices.
USES: This method will recover most nematode eggs and protozoan cysts,
however many trematode and cestode eggs, as well as Giardia cysts will not be
recovered.
Specific Gravity of Some Helminth Eggs as Determined Using Sucrose Density
Gradient Centrifugation*
Species
Range
Ancylostoma caninum
1.0559
1.0549 - 1.0573
Toxocara canis
1.0900
1.0791 - 1.0910
Toxocara cati
1.1005
1.1004 - 1.1006
Taenia sp.
1.2251
1.2244 - 1.2257
Physaloptera sp.
1.2376
1.2372 - 1.2380
ZnSO4 Solution
1.18
1.20
1. The sieve must be in the liquid in order for the feces to be passed through.
2. The more feces you use, the more likely you will be able to find eggs which are
present in low numbers.
To increase the sensitivity: After removing the tube from the centrifuge, fill the
tube with ZnSO4 to just
3. Place a coverslip over the top of the tube and wait 5 to 10 min. This modification
also allows you to skip using the loop or headed rod to obtain your sample, and
thus may be easier to do at a veterinary practice.
4.If the sample contains a large amount of fat or other material that floats in
water, you may want to wash the sample before doing the flotation. To do this,
start at step 1 but use water instead of ZnSO4. When you centrifuge the waterfecal mixture the eggs ,being heavier than water, will sink but the fat and other
material will float. After centrifugation pour off the supernatant, add the
ZnSO4 solution and mix well. Centrifuge as in step 4 and examine as in step 5.
PREPARATION OF ZNSO4 SOLUTION:
Add 386 grams of ZnSO4 to 1 liter of water. The mixture should be checked with a
hydrometer and adjusted to 1.18 sp. Gr. The ZnSO4 solution should be stored tightly
capped to prevent evaporation (and the resulting change in the specific gravity of the
solution).
PREPARATION OF THE IODINE SOLUTION:
Add 10 gms Potassium Iodide (KI) to 1 liter of distilled H2O. Shake to dissolve.
Add 10 gms of Iodine (I2) to the above solution. Allow to stand over-night with
stirring, at this time you may still have Iodine crystals at the bottom, this is OK,
just leave them there. This solution will stain (and kill) most parasite eggs and
cysts (coccidial oocysts are an exception, they do not take in the iodine).
ETHYL ACETATE SEDIMENTATION
METHOD:
1. Pass a grape-sized piece of feces through a sieve into about 9 ml of water and pour
into a 15 ml centrifuge tube.
2. Add about 3 ml of ethyl acetate, plug the tube with a rubber stopper and shake the
tube vigorously.
CAUTION: Test materials before placing Ethyl Acetate into them. This solvent will
dissolve many types of plastic!! The white plastic centrifuge tubes (polypropylene) used
in the lab are OK, but clear hard plastic tubes and the disposable polystyrene cups will
dissolve.
3. Remove the rubber stopper and centrifuge the tube (1500-2500 rpm) for 1 to 2
minutes.
4. Using a stick, "ring" the plug of fat at the water - ethyl acetate interface (the plug
adheres to the side of the tube and must be detached before the liquid contents of the
tube can be poured off).
5. Pour off the supernatant, being careful to leave the pellet at the bottom of the tube
intact. (Flush the ethyl acetate down the sink with plenty of water.)
6. Transfer some of the sediment from the bottom of the tube to a slide and examine.
The sediment can be transferred in several ways: 1) If some liquid remains, the pellet
can be resuspended and a drop transferred with a pipette. 2) Add a drop of iodine to the
pellet to resuspend it and then transfer with a pipette. 3) Use a stick to remove some of
the pellet and smear it on a slide as you would when making a direct smear.
Note: For this technique to be as sensitive as a flotation method, you must examine the
entire pellet!
NOTE: When removed from centrifuge, your tube will have clearly defined layers:
1. ethyl acetate layer on top
2. plug of dissolved fat in the middle
3. layer of water below fat
4. pellet of sediment on bottom
USES: Used when the feces contain a large amount of fat
(which make use of a flotation method difficult) or when
you are specifically looking for trematode or cestode eggs.
Because formalin fixed eggs and cysts may not float (they
may now have a specific gravity of greater than 1.2) this
technique is preferred for formalin fixed samples.
TIP: If you did this technique just to remove fat, you can
resuspend the pellet in flotation solution, centrifuge, and
remove the material from the top of the float to examine
for eggs.
Baermannization:
In 1917, while working in Java, the Dutch physician Dr. Baermann developed a simple
method for isolating nematodes from soil. Today veterinarians use his method for the
extraction of live larval stages of nematode parasites from the feces.
1. Place a sieve in a custard dish or other similar container.
2. Spread about 10 grams of fresh feces on a piece of tissue paper and place it into the
sieve.
Note: Since you are looking for live larvae, anything which is done to the feces that
might kill the larvae should be avoided (i.e. don't let it dry out, don't put it into
formalin, don't freeze it, and even keeping it in a refrigerator overnight may impair the
larvae's motility).
3. Place warm water* in the custard dish until it just covers the feces, taking care not to
disrupt the feces.
4. Allow to sit for about one hour**.
5. Lift off sieve and pour liquid into a 50 ml centrifuge tube.
6. Let sit for 20 minutes.
7. Using a Pasteur pipet, remove a drop of the sediment at the bottom of the tube and
place it on a microscope slide for examination. (Be careful not to resuspend the sediment
before you take a sample from it.)
USES: This technique is used to recover larval nematodes for identification. Larval
nematodes are normally not numerous in feces and therefore not seen on a direct smear
or sedimentation. They are also damaged by flotation solutions, making identification
difficult to detect them. This technique makes use of two characteristics of parasitic
larval nematode behavior:
1. The warmer it is the more active the larva (up to a point: 37 to 400C is as warm
as you want to get. You dont want to cook them!). Also, some nematode larvae
are thermotaxic and will move toward the warmer water in the funnel (the
surface cools quicker than the middle of the funnel).
2. Most parasitic larval nematodes are poor swimmers.
Therefore, the following events take place when the sieve is placed in the water:
The larvae will be moving around in a random fashion and within any given time
interval some of them will migrate through the tissue and fall into the water. Because
they can't swim, they sink to the bottom and over time a number accumulate there. The
more active (or themotaxic) the larvae are (i.e. the warmer the water) the greater the
number of larvae that will accumulate at the bottom in a given time interval.
The longer you wait the more larvae will fall to the bottom of the dish, but with time the
fecal sample also breaks down, leading to an accumulation of sediment along with the
larvae.
Techniques
Advantages
Direct Smear
Quick to prepare.
Disadvantages
No distortion of parasites if
isotonic saline is used as diluent.
the diluent).
Saturated Salt
coccidian oocysts.
Flotation
performed.
Zinc sulfate
floatation
especially Giardia.
Ethyl acetate
sedimentation
protozoan cysts.
EXAMINATION OF FAECES
Direct Smear Method
In this method a clean and dry glass slide is used which should be free from scratches.
Place a drop of distilled water in the middle of the slide and add small amount of faeces
on it. Mix with tooth pick or match stick and place a coverslip on it. Examine it under
microscope for the presence of parasitic ova. In direct smear method, if no parasitic ova
is detected then it should be examined by qualitative or quantitative methods before
being declare it as negative.
33% zinc sulfate solution. The parasitic ova, being lighter float on the top of fluid and
thus can be concentrated for examination.
A. Simple floatation: In this method, about one gram of faeces is taken and
mixed with few ml of distilled water and is filtered through a fine sieve or
muslin cloth. The filtrate is then mixed with 4-5 ml of saturated salt solution.
It should be then placed in a tube or cylinder and filled up to the top with
solution. A clean glass slide or cover-slip is placed on the mouth of tube or
cylinder. This is left for 30 to 60 minutes at room temperature without any
disturbance.After that coverslip or slide is removed and examined under
microscope for the presence of parasitic ova. The unfiltered material left on
sieve or muslin cloth is examined for tapeworm segments or/parasite.
B. Centrifugation floatation: In this method about one gram of faeces is mixed
with distilled water and filtered through a fine sieve or muslin cloth. The
filtered material is mixed with saturated sugar solution in a ratio of 1:3 in a
test tube. Mix the contents and centrifuge at 1500 rpm for 5 minutes. The
tube is taken out and placed on stand without any disturbance. Transfer the
small amount of superficial contents of tube on a clean and dry glass slide
and examine under microscope for the presence of parasitic ova. The
sediment of the floatation method is examined under microscope for eggs of
liver flukes
Ancylostoma
Toxocara Leonina
Toxocara
Canis
Echinococcus
Trichuris
Spirocera lupi
unsporulated
Diphyllobothrium
Fasciola Egg
Toxoplasma oocyst
Diphylidium
Caninun
latum
Strongyle Egg
Name of parasite
Symptoms
Diagnosis
Trematodes:
1. Paragonimus
kellicotti
Intermittent cough
2. Heterobilharzia
americana.
Nematodes:
Intestional
nematodes
1.Ascarid
diarrhea (which
may be bloodtinged), vomiting
fenbendazole
5o mg /kg
10 to 14 days
Praziquantel
Praziquantel
fenbendazole
(24mg/kg PO sid
for 7 days)
Pyrantel
5 mg/kg (PO)
Fenbendazole
pyantel
ivermectin
Fenbendazole
Fenbendazole
Pyrantel
Butamisole
Fenbendazole
Zoonotic
importance
dermatitis in
humans
(swimmers
itch)
Normally found in
young puppy
pot-bellied, with
abdominal pain,
a. toxocara canis
b. toxoascars leonina
Sedimentation
with saline in
faeces
Drugs of choice
Diarrhea and
vomiting.
Baermann
Technique, Zinc
visceral larva
migrans
floataion
c. strongyloides
species
pot-bellied, and
have
Diarrhea, in auto
infection troubled
breathing
verminous
pneumonia
Urine
Sedimentation.
fecal flotation
fecal flotation
2.Hook worms
Ancylostoma
caninum
3.whipworms
Trichuris vulpis
Anemia, pale
membranes,
diarrhea
Bouts of diarrhea,
often with large
amounts of mucus
and frank blood on
the stool.
fecal flotation
Cutaneous
larval
migrans
Non Intestinal
nematodes:
a. Dirofiliria Immitis
(Heart worm)
Cough, dyspnoea,
tachycardia, caval
syndrome, right
heart failure,
hypertension,
exercise intolerance
Difil Test or
Knotts Test
b. spirocerca lupi
(oesophageal worm)
vomiting with
worms, difficulty in
swallowing
Fecal
Flotation,Trach
eal wash
c. Dioctophyme
renale
( giant kidney worm)
Asymptomatic and
sometimes
vomiting
d. Cappilaria spp.
( lung worm)
rattling and
wheezy breathing
and some
coughing.
e. Thalazia
Callipaeda
( Eye worm)
Blepharospasm
and epiphora.
Eggs may be
found in faeces
or vomit
otherwise
diagnosis may
be by
endoscopy or
radiography.
Fecal floataion
Trachea wash
detection of
adult or larval
stages from
samples of the
tear film
ivermectin
Diethycarbamizi
ne
Surgical removal
Ivermection
Fenbendazole
Ivermection
Fenbendazole
Ivermection
Zoonotic
3.Cestodes
a)Ecchinococcus
granulossus
Praziquantel
30mg/kg
Epsiprantel
Usually
asymptomatic in
the dog.
b) Diphyllobothrium
latum
c) Diphylidium
Canninum
Fecal floatation
Disease
Nematodesa) Ascariasis
Symptoms
The calves with ascariasis
remain unthrifty and pass
large load of worms in the
faeces at periodic
intervals. The calves may
show convulsions and
indigestion as main
symptoms and normally
pass foul smelling clay
colored or watery feces. A
characteristic butyric
odour may also be
detectable in their breath.
b)Strongyloidosis
Intermittent diarrhoea
with blood and mucus is a
common feature. Build-up
of warm and moist areas
should be prevented inside
shed.
2.Trematodes-
a)fasciolosis
Treatment
Piperazine compounds provide prophylaxis towards all the
round worms effectively including Neoascaris. Piperazine
hydrate (56.3% w/v) is administered right from day 3 @ 3-6
ml per 10 kg body weight.
b)Amphistomes
anorexia, polydipsia,
unthriftiness, and severe
diarrhea, shooting foetid
diarrhoea
albendazole at 1520 mg/kg in a single dose or two doses of
7.5 mg/kg on successive days, or netobimin at 20 mg/kg.
c)Dicrocoelium
dentriticum
condition, recumbency,
hypothermia, and anemia,
diarrhea
3.Cestodes
a. Moniezia Sp
Coccidiosis
Ectoparasites
CHAPTER 11
VACCINOLOGY
Precaution to vaccination:
1. Vaccinate only healthy animals
2. Avoid Animal with malnutrition, helminth infestation,
3. Refrain from use of administration of immunosuppressive agents like corticosteroids, radiation
therapy, etc. will suppress immune response to vaccine;
4.Injection site is important - follow Manufacturers Instruction
5. In rare cases hypersensitivity may occur, immediate treatment with antihistaminics is advocated
Manufacturer
adjuvant
Dose
Primar
y
Booster
revaccinatio
n
Intervet India
Binary
ethyleneimine (BEI)
inactivated FMD
mineral oil emulsion
vaccine containing a
mixture of virus
serotypes O, A and
Asia-1
Inactivated tissue
culture FMD virus
strains O, A and
Asia-1 adsorbed on
Al (OH)3gel and
saponin as an
adjuvant
Inactivated tissue
culture FMD virus
strains O, A, and
Asia-1 adjuvanted
with mineral oil
Formaldehyde
inactivated culture
ofPasteurella
multocidaadsorbed
on aluminium
hydroxide gel
2ml, i/m
(Vial: 100 ml)
3 months
onwards
After
4-6
weeks
of
primary
vaccination
II After 24
weeks of
first booster
Every 4 to 6
months after
2nd booster
vaccination
3 ml in the
mid-neck
region, s/c
(Vial: 30 ml)
4 months
weeks after
primary
vaccination
Every 4to 6
months after
booster and every 4
months in endemic
areas
2 ml in
the mid-neck
region, deep
i/m
4 months
9 months
after
primary
vaccination
Annually
6 months
and above
FMD
Bovilis Clovax
Raksha
Indian
Immunologicals
Raksha Ovac
Indian
Immunologicals
Haemorrhagic
Septicemia
Indian
Immunologicals
Raksha HS
Compound Vaccine
Infection
Manufacturer
Raksha biovac
(FMD+HS)
Raksha triovac
(FMD+HS+BQ)
Indian
Immunologicals
Indian
Immunologicals
adjuvant
Dose
Primar
y
Booster
revaccination
3
ml,
midneck,
deep i/m
(Vial: 30 ml)
4
months
9 months
Annually
4
months
9 months
Annually
multocidaculture,
inactivatedClostridium
chauvoeiculture mixed
together in light mineral
oil emulsion
Formaldehyde inactivated
cultures ofPasteurella
multocidaand Clostridium
chauvoeiadsorbed on
aluminium hydroxide gel
(Vial: 30 ml)
6
months
and
above
4-8
months
old
serologic
ally
negative
female
calves
Raksha HS+BQ
Indian
Immunologicals
Bruvax
Indian
Immunologicals
Live Brucella
abortusstrain 19 freeze
dried bacteria, each dose
40x109 organisms
2 ml., s/c
(Vial: 5 dose
freeze dried
vaccine with
10 ml sterile
ciluents)
RakshaAnthrax
(Prophylactic
only)
Indian
Immunologicals
1 ml, i/m or
s/c
(Vial: 50 ml)
Sterne Vaccine
institute of
Veterinary
Preventive
Medicine, Tamil
Nadu
1 ml, s/c
Revaccinate
after 2-3
weeks in
heavily
contaminated
areas
annual vaccination in
endemic areas
Depending upon
prevalence in a given
area, vaccination
against following
diseases may also be
taken up Anthrax
Note: Protect animals from overexertion 3 days following anthrax vaccination. Do not vaccinate the animal 60 days before slaughter
Rabies
Raksharab,
Prophylactic
Indian
Immunologicals
Post-exposure
therapy (PET)
In case
3 years, annual
primary
vaccination recommended
vaccination
in endemic areas
is given
below 3
months of
age, a
booster dose
should be
given at 3
months age
I-Day Zero of dog bite or within 24 hrs, II-Day 3, III-Day 7, IV-Day 14,
V- Day 28 and VI-Day 90
1 ml by
s/c or i/m
route
(Vial:
1
ml, 5 ml,
10 ml)
3 m and
above
Manufacturer
Primary
Booster
Dose
revaccination
Enterotoxae
mia
Raksha ET
Intervet
21 days
after
booster
S/c 2 ml
Tetanus
days
Before weaning
or before
monsoon
3 rd months
administered simultaneously
After 21
S/c
Annually
days
-
S/c 1 ml
3 years once
Goat pox
vaccine IVRI
3 rd month
1 year once
Raksha
SP/Sheep pox
vaccine (50 ml)
Indian
immunologicals /
Intervet/ Indian
immunologicals /
Intervet/IVBP
Raksha FMD
3 rd
Caudal
fold of tail
1 ml sc
1ml sc
4th months
Deep im
6 months
5 to 8 months
s/c
1ml Sc
Pastuerollosis
Raksha HS
PPR vaccine
Raksha PPR/
Tissue culture
PPR Vaccine ,
(IVRI)/ IVPM
Goat pox
Sheep Pox
FMD
Brucella
Bruvax Rev1
Anthrax
Intervet /IVBP
6 months
1 year once
Mode of
administration
Marek's disease
1 day
SC
Newcastle/infectious bronchitis
14 21 days
Water
1421 days
Water
Newcastle/infectious bronchitis
5 wk
Newcastle/infectious bronchitis
810 wk
Encephalomyelitis
1012 wk
Wing web
Fowlpox
1012 wk
Wing web
Laryngotracheitis
1012 wk
Intraocular
Mycoplasma gallisepticumb
1014 wk
Intraocular or spray
Mycoplasma gallisepticumb
or 18 wk
Parenteral
Newcastle/infectious bronchitis
1214 wk
Water or aerosol
Newcastle/infectious bronchitis
1618 wk
Water or aerosol
Newcastle/infectious bronchitis
Parenteral
Mode of
administration
Marek's diseaseb
1 day
SC
Newcastle disease
1 day or
Coarse spray
1421 days
1 day or
Coarse spray
1421 days
1421 days
Water
Infectious bronchitis
Presentation :
Dose :
5 to 10 ml for cattle & buffaloes
depending upon the size of the animal.
5 ml for
sheep & goats
Route of Administration: Through subcutaneous
Age:
Immunity:
Six months
Dose: 5 to 10 ml for cattle & buffaloes depending upon the size of the animal
Route of Administration:
Age:
Immunity:
Through subcutaneous
Description:
Prepared from highly toxigenic strain of clostridium perfringens type D grown in
anaerobic fluid medium, activated by trypsin, rendered sterile and atoxic by addition
of formalin and precipitated by addition of alum.
Periodical vaccination in endemic and outbreak areas
Presentation:
Dose:
250 ml bottles
Three to five ml.
Route of Administration:
Age:
Through subcutaneous
vaccinated
Immunity:
Six months
6.Poultry vaccines
Ranikhet DiseaseRDVF, RDVK (kumorovs strain),
RDV (Lasota strain) and
Duck plague vaccine
Description:
Egg adopted vaccines Prepared out of living ranikhet disease virus of high antigenicity
and low virulence strains by freeze drying the emulsion of embryo and fluids from
infected embryonated eggs.
Transportation of Poultry vaccines:
The vaccine should be transported on ice in thermo cool boxes.
Storage and Keeping quality:
The vaccine can be stored at -20c in deep freezer for one year.
When transported on ice and stored in freezing chamber of refrigerator , the
vaccine can be used up to 2months from the date of despatch.
,lk;:
Njjp:
1.
2.
Njitahd msT
3.
4.
5.
(my;yJ)
ghHry; %yk;
Fwpg;Giu:
,uhzpg;Ngl;il fhy;eil Neha; jLg;G kUe;J epiya ,af;Feupd;
tpepNahfj;jpd; nghUl;L gzpe;jDg;gg;gLfpwJ.
Njit NfhUgtupd; ifnahg;gk;:
(Kj;jpiuAld;)
mYty; ngaH
jiyikaplk;
ftdpg;G:
:
:
NkYk;
CdPiu xU khjj;jpw;F NkYk; itj;jpUf;ff;$lhJ.
jdpj;J}Jtupd; khjpup ifnahg;gk;:
1.
2
CHAPTER12
BIOSECURITYMEASURES
TYPESOFDISINFECTANTSUSEDINANIMALDISEASECONTROL
Disinfection is the process of eliminating infectious organisms by using
chemical or physical agents. The antimicrobial agents designated as disinfectants are
sometimes used alternatively as sterilizing agents, sanitizers or antiseptics. For the most part,
disinfectants used in animal health are relatively strong, usually toxic antimicrobial or
biocidal chemicals, and are applied to contaminated surfaces. Those used in food processing
are usually less toxic and more diluted. Modern disinfectants are complex formulations of
chemicals, soaps, detergents, and compounds which improve penetration of the active
ingredients.
These include hot water, acid-anionic surfactants (substances that improve
penetration by reducing surface tension), amphoteric surfactants, bromides, chlorides,
Chlorhexidine, iodides, phenolic compounds and quaternary ammonium compounds.
Water
Water is the most important element of the HACCP concept and of the cleaning
and disinfection process. Although water is actually a cleaner rather than a disinfectant, hot
water has major disinfecting applications. Hot water is sometimes the major component of
cleaning and disinfection in abattoirs and food-processing plants, where chemical residues
must be avoided. Hot water under pressure cleans by flushing and by hydraulic impact; it
dissolves inorganic salts, emulsifies fats, washes away organic debris, and is briefly
bactericidal until the surface cools.
Ammonium hydroxide
Ammonium hydroxide is effective against oocysts of Coccidia spp., which
affect poultry and rabbits. This substance is not effective against most bacteria. To obtain
Chlorhexidine
Chlorhexidine and its analogs are commonly used at concentrations below 4% as
skin cleaners, teat dips and antiseptics; they are also used for cold sterilization of surgical
instruments and for disinfecting equipment, barns and buildings. While Chlorhexidine is not
sporicidal, it is useful against fungi, Gram-positive bacteria and, to a much lesser extent,
against viruses and Gram-negative bacteria (some of which are resistant to Chlorhexidine).
Chlorhexidine retains some activity in the presence of small amounts of uncontaminated
organic material (e.g. milk, serum or fish meal) but is ineffective when gross faecal
contamination is present. The low toxicity of Chlorhexidine makes it useful in combination
with other disinfectants. Chlorhexidine has broad applications in cleaning dairy equipment
and in aquaculture.
Iodine and iodine-based disinfectants
Many forms of iodine find common use in animal health and food-processing
disinfection. In nature, iodine is always found in combination with other elements. It is
present at high levels in seaweed, which is the most common commercial source; iodine is
also found in extractable levels in sea water and other brines, and in nitrate deposits (where it
exists as iodates). In the pure state, iodine is a soft black crystalline solid. Low levels of
iodine are essential to mammalian life, and deficiency results in goiter. In great excess, iodine
can cause acute or chronic toxicity. Aqueous iodine (Lugol's solution) or alcoholic iodine
solutions (tinctures of iodine) are commonly used as antiseptics.
Iodophors
Iodophors are disinfectants formed by combinations of iodine with various carrier
compounds. These release iodine in an acid medium and have disinfectant properties which
affect bacteria, viruses and some spores . Iodophors are used for general disinfection and
cleaning, bovine teat dips, and surgical scrubs. Hard water and the presence of large amounts
of organic material reduce the activity of iodophors, but these disinfectants can function
against salmobnella, E.coli infection, and pasteurella infection since it is bacteriocidal.
Quaternary ammonium compounds
Quaternary ammonium compounds (QACs; also called 'quats') are natural
biochemicals involved in the transmission of neuromuscular impulses in mammals.
Synthesized QACs are surface-active cations which have sanitizing/disinfecting and mild
Organic acids
A number of organic acids with bactericidal and mild viricidal properties have
disinfectant applications in animal health and food processing, as they are less toxic and less
corrosive than the inorganic (metallic) acids mentioned above. Acetic acid is readil
available, being present (4%) in vinegar. At 2%, acetic acid can significantly reduce levels of
FMD virus on contaminated surfaces, and is used to reduce bacterial levels in meat packing
plants. Acetic, citric, lactic, and formic and propionic acids are sometimes used in meat and
poultry packing plants, and in calf and pig barns. These acids have also been added to animal
feeds to reduce levels of Salmonella contamination
Formaldehyde
The natural form of formaldehyde is a gas. However, formaldehyde is more readily
available as a 40% aqueous solution called 'formalin'. Gaseous formaldehyde is used for the
fumigation of buildings, rooms or vehicles which can be sealed. Fumigation with
formaldehyde is effective against most viruses and bacteria, including the acid-fast
Mycobacteria. Formaldehyde gas is relatively unstable and can sometimes explode. It is
difficult to achieve an even distribution and penetration of formaldehyde gas throughout
buildings, which may lead to incomplete effect (13). For formaldehyde fumigation to be
complete, the temperature must be above 55F (13C) and relative humidity must be above
70%. Spraying with hot water is sometimes necessary to achieve these conditions. For
fumigation purposes, formaldehyde gas can be produced by oxidizing formalin with
potassium permanganate, by heating paraformaldehyde, by mechanically generating a mist of
formalin, or by applying complex mixtures from which formaldehyde is slowly released after
application. Formaldehyde fumigation is hazardous and must be carefully supervised. A 1-5%
formalin solution is sometimes used to disinfect buildings or as a prophylactic and
therapeutic foot bath for foot rot in sheep and cattle. The use of formaldehyde in disinfectant
situations is declining, due to the strong, irritant odour, corrosiveness, fibrolytic properties
and toxicity.
Glutaraldehyde
Glutaraldehyde, which has been used for cold liquid sterilization of surgical
instruments, is sometimes still used to disinfect surfaces. As with formaldehyde, the use of
glutaraldehyde is diminishing in favour of newer products.
Sodium carbonate
Sodium carbonate, also known as washing soda, has been used in a hot solution
(180F [82C]) for disinfecting buildings which have housed animals with FMD. This
substance lacks efficacy against some bacteria and most viruses, including Newcastle disease
virus. Sodium carbonate is more effective as a cleanser than as a disinfectant. In case of FMD
outbreak 4 percent sodium carbonate can be used as disinfectant.
LIST OF DISINFECTANT FOR DECONTAMINATION:
Disinfecta
nt group
Form
Strength
Usual
Final
dilution
As
appropri
ate
Soaps and
detergents
Solids or
liquids
Sodium
hypochlorite
NaOCI
Conc.
liquid (10
12%
available
chlorine)
1:5
Calcium
hypochlorite
Ca(OCI)2
Solid
30 g/litre
Vikron
Powder
20 g/litre
Contac
t time
10 min
23%
available
chlorine
(20
000&ndas
h;30 000
ppm)
1030
min
Applications
and virus
category
Thorough cleaning
is an integral part
of effective
decontamination.
Use for category A
viruses.
Use for virus
categories A, B,
and C. Effective
for most
applications
except when in
the presence of
organic material.
Less stable in
warm, sunny
conditions above
15C.
1030
min
23%
available
chlorine
(20
000&ndas
h;30 000
ppm) 2%
(w/v)
10 min
Sodium
hydroxide
Pellets
20 g/litre
2% (w/v)
10 min
Sodium
Powder
40 g/litre
4% (w/v)
10 min
Excellent
disinfectant active
against all virus
families.
Very effective
against virus
categories A,B and
C. Do not use in
the presence of
aluminium and
derived alloys.
Recommended for
carbonate
anhydrous
(Na2CO3
washing
soda
(Na2CO3.10H
2O)
use in the
presence of high
concentrations of
organic material
Crystals
100
g/litre
10% (w/v)
30 min
Hydrochlori
c acid
Conc. acid
(10 Molar)
1:50
2% (w/v)
10 min
Citric acid
Powder
2 g/litre
0.2% (w/v)
30 min
Glutaraldeh
yde
Conc.
solution
as
appropri
ate
2% (w/v)
1030
min
Formalin
40%
formaldeh
yde
8%(w/v)
1030
min
1:12
CHAPTER-13
The biggest impediment to growth of this sector, however, is the large-scale prevalence of
diseases such as Foot and Mouth Disease (FMD), Haemorrhagic Septicaemia (HS), Black
Quarter (BQ) in cattle, Enterotoxaemia, Peste des Petits Ruminants (PPR) & Sheep-Goat Pox
in sheep and goats and Swine Fever in pigs, which drastically affect the productivity of
animals. The presence of animal diseases also deters domestic and foreign investment in the
livestock sector. These diseases not only wreck havoc on the existing stock but also constrain
market access to our livestock sector, in spite of the fact that we have ample scope to
participate in the global trade.
The economic impact of the diseases in livestock results from both morbidity and mortality
and the consequent production losses. This includes the direct losses due to mortality, reduced
production in terms of milk, meat, wool, hide and skins, as well as indirect loss due to
abortions, subsequent infertility, sterility, and deterioration of semen quality. Controlling
animal diseases is of upmost important for to prevent these losses and livestock industry to
progress for the benefit of the livestock farmers.
At present, an animal disease is primarily recorded by the veterinary doctor working in a
Government hospital / dispensary on the basis of clinical diagnosis. This information is
passed on to the Taluka / Block level and then to the District and the State veterinary
authorities. Disease information is also generated from the disease diagnostic laboratories at
the District, State or regional level on the basis of laboratory diagnosis. Finally, information
from State level is transmitted to the Central Government, i.e., the Department of Animal
Husbandry, Dairying & Fisheries (DADF) in New Delhi. The DADF notifies the World
Animal Health Organization (OIE) and other international organizations, as appropriate.
The present system of animal disease reporting is not satisfactory for the following reasons:
The disease reporting is neither timely nor complete. As a result of reliance on
postal means of communication, the reports and returns take considerable time and some are
also lost in transit. Hence, the compiled information does not represent true picture of the
disease situation at any given point of time.
The veterinary services available in the country are grossly inadequate. As a
result, a large portion of the livestock owners do not have access to the Government
veterinary services. These people rely on either the traditional systems of veterinary medicine
or the private veterinary services. These incidences of animal diseases remain out of the
reporting system. Their number is believed to be significant. In the prevailing situation, many
times animal diseases assume serious proportion before control and containment steps can be
initiated, thereby causing avoidable social and economic costs on the livestock owners and
the countrys economy.
In order to bring about desired change to the existing situation, a computerized
system of animal disease reporting is being introduced, linking each Taluka / Block, District
and State Headquarters to a Central Disease Reporting and Monitoring Unit at the DADF in
New Delhi. The diagrams given below depict the NADRS contemplated diagrammatically,
along with various agencies who would be expected to contribute data to the system and its
transmission to the Central Monitoring Unit in the DADF at New Delhi.
The reporting system envisaged will enable the Block, District and State animal
health officials to report the disease information and render reports and returns prescribed in
this regard via internet. The system will be so designed as to assure secure data transfer and
confidentiality of information. At the apex level, NADRS will compile and generate animal
disease information for the country as a whole. The users will have access to the information
as per permissions in consonance with their role and responsibilities envisaged under the
system. This computerized system, proposed to be called National Animal Disease Reporting
System (in short NADRS), will enable fuller and timely reporting of the animal disease
situation in the country, enabling its effective management.
District Unit
District Laboratory
NGOs
Stockman/sub-centers
Local bodies
Farmers/ Livestock Owner
As a result of the information that would emerge from the NADRS, it would
be possible to develop disease forecasting models, leading to development of disease
prevention strategies. As the proposed scheme aims at effective monitoring the occurrence of
livestock diseases with a view to enabling their early control, it will result in improving the
livestock health in the country. By the very nature of the benefits that would accrue, these
cannot be quantified in concrete terms. There is, however, no doubt that implementation of
the scheme will yield immediate benefits to the livestock owners and to the economy by way
of better health status of animals, prevention of losses due to their morbidity and mortality
and improvement in the quality of their products. The benefits likely to accrue to livestock
owners and to the economy may be summarized below:Benefits to livestock owners
Benefits to economy
In the beginning the users may find the data entry forms exhaustive, but this is because
these are designed to capture all details about the disease reporting. If filled properly,
in long run these will be beneficial in the future activities like generating detail reports
without going through the manual exercise of referring different registers maintained
for it. It would create a very good data bank about animal diseases which can be
further used for analytical purposes.
CHAPTER -14
ASSISTANCE TO STATE FOR CONTROL OF ANIMAL DISEASES (ASCAD)
A Centrally Sponsored Project with 75% Central & 25% State Assistance. To be
implemented in the state during 10th plan.
Objectives
1. To control emerging diseases, exotic diseases and existing diseases of the State.
2. Training of field veterinarians as well as field Para-veterinarians.
3. Surveillance and Monitoring of diseases and preparation of disease forecasting
model
Information and Communication campaign and Community participation
Immunization of animals against economically important diseases like Foot and
Mouth Disease (in areas not covered under FMD-CP), Haemorrhagic septicaemia, Anthrax,
Black quarter, Peste des petits Ruminants (PPR), Enterotoxaemia, Sheep & Goat Pox, Swine
fever, Ranikhet diseases, Marek,s diseaseand Duck plague.
Earlier the diseases covered were only of zoonotic importance. Now the thrust is
also on preventive and curative thus leading to control and eradication of diseases, especially
the transmissible diseases having the potential for very serious and rapid spread irrespective
of national borders, which are of serious socio-economic or public health consequence and of
major importance in the international trade of animals. The activities to be covered under this
component are given as under:
Strengthening / modernization of Biological Production Units/ State Disease
Diagnostic Laboratories. This will include minor alteration / modification of the existing
laboratories / units, purchase of tools and equipments, Computers, Photocopier, Air
conditioner, Fax etc. State-wise number of Biological Production Unit/ Disease Diagnostic
laboratory to be strengthened / modernized
Strategic immunization of Livestock and Poultry against economically imporrtant
diseases. Strategic immunization would depend essentially on the epidemiology of the
disease in various States. /Uts. And the tools available to control and eventually eradicate the
disease. This will include expenditure on cost of vaccine and diagnostic, maintenance of cold
chain & Transportation.
Disease Surveillance, Monitoring and Forecasting.
This will include collection of information on the incidence of various livestock and poultry
diseases from States and Union Territories and compiling the same for the whole country. The
CHAPTER -15
Physiological Parameters & Probable Diseases in Animals
1 . Animal Disease and Body Temperature Chart
Sr.
No.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13
14
15
16
17
18
Disease
Temperature
19.
Heat stroke
103-109F
Anaplasmosis
Anthrax
Babesiosis
Black quarter
Blue tongue in sheep
Bovine ephimeral fever
Bovine malignant
catarrh
Bovine viral diarrhea
Braxy in sheep
Calf pneumonia
Camel pox
Contagious bovine
Pleuropneumonia
Fern poisoning
Fog fever
Foot and mouth disease
Foot rot
. Glassers disease in
pig
Hemorrhagic
septicaemia
<105F
107F
106F
106F
105-106F
105-106F
106-107F
20.
21.
22.
23.
24.
25.
26.
105-107F
105F
105.8-107.6F
101-106F
107F
105-107F
105F
105-106F
>107F
104-105F
103-105F
105F
27.
28.
29.
30.
31.
97-101F
103F
105-107F
103-105F
105-107F
105-109F
106-108F
104-106F
103-104F
104-107F
32.
33.
34.
35.
36.
Milk fever
Pyelonephritis
Rinderpest
Rabies
Salmonellosis (calves and
pigs)
Sheep pox
Strangles
Swine influenza
Theileriosis
Toxoplasmosis
>105F
103-105F
107F
106F
(pigs) 104-107F
106-107F
GAIT
DISEASE/DISORDER
Azoturia
4
5
Tetanus
Coenurosis(gid); Otitis: Listeriosis
Cerebello-Vestibular Disorder
Stumbling gait
10
11
12
13
14
15
16
17
18
Knuckling of fetlock
Posture
A Cow sits on sternum and the head rests
on the flank
Disease/Disorder
Milk Fever (Hypocalcaemia)
Obturator paralysis
10
11
Pneumonitis
12
Traumatic pericarditis
13
Percarditis
14
15
Hypomagnesaemic - Tetany
16
Tetanus.
17
Cystitis, Urolithiasis
18
Pneumonitis, Pleurisy
19
Acute nephritis
20
Dog Schooting
21
Pregnancy Toxaemia.
22
Polyoencephalomalacia (Thiamine
Defeciancy): Gid
23
Parurient Hypocalcaemia.
24
Infective Arthritis
25
Scrapie.
26
27
Muscular dystrophy.
Common Names for the Sex, Young, Group and Birthing of Animals.
Animal
Antelop
1 e
Male
Female
Young
Group
Giving
Birth
Buck
Doe
Kid
Herd
Kidding
2 Bear
Boar
Sow
Sleuth
Cubbing
3 Bird
Cock/Stag
Hen
Cub
Fledging/
Nesting
Flock
Hatching
4 Bison
Bull
Cow
Calf
Herd
Calving
5 Bobcat
Tom
Lioness/Queen
Kitten
Litter
Littering
6 Cat
Tom
Pussy/Queen
Kitten
Clowder
Queening
7 Cattle
Cow/Heifer
Calf
Herd/Drove
Calving
8 Chicken
Bull
Rooster/
Cock
Hen/Pullet
Chick
Flock
Hatching
9 Deer
Buck/Stag
Doe
Fawn
Herd
Fawning
10 Dog
Dog
Pup/Puppy
Kennel
Whelping
11 Donkey
Jackass
Bitch
Jennet/
Jennyass
Colt
Herd
Foaling
12 Duck
Elephan
13 t
Drake
Duck
Duckling
Flock
Hatching
Bull
Cow
Calf
Herd
Calving
14 Fox
Reynard
Vixen
Cub/Pup
Earth/Skulk
Pupping
15 Giraffe
Bull
Cow
Calf
Herd
Calving
S.N
16 Goat
Billylbuck
Nanny
Kid
Trip
Kidding
17 Goose
Hog/Swi
18 ne
Gander
Goose
Gosling
Gaggle/Flock
Hatching
Boar
Sow/Gilt
Piglet/Shoat
Foal/
Colt (male) /
Filly (female)
Herd/Drove
Farrowing
Stable/Herd
Foaling
Joey
Troop/Herd
Cub
Pride/Flock
Whelping
Chick
Flock
Hatching
Howlet/Owlet
Flock
19 Horse
Kangar
20 oo
Stallion/
Stud
Buck/
Bommer
21 Lion
Lion/Tom
Doe/Flyer
Lioness/She
lion
22 Ostrich
Cock
Hen
23 Owl
Owl
24 Ox
Steer
Cow
Stot
Herd/drove
Calving
25 Rabbit
Buck
Doe
Kitten
Colony
Kindling
26 Rat
Buck
Doe
27 Seal
Bull
Cow
28 Sheep
Buck/Ram
Ewe/Dam
Pup
Lamb/Lambk
in
29 Turkey
Tom
Hen
30 Walrus
Bull
Cow
Mare/Dam
Colony
Herd/Harem/
Rookery
Flock/Hurtle
Lambing
Poult
Flock
Hatching
Cub
Herd
1
2
3
4
5
6
7
Species
Livestock
Ass
Cattle
Goat
Horse,Heavy
Horse, Light
Days
365
280-290
148-156
333-345
330-337
1
2
3
Pig
Sheep
Pets
Cat
Dog
Guinea pig
Hamster
Mouse
Rabbit
Rat
Fur Animals
Chinchilla
Ferret
Fisher
105-115
42
338 358
4
5
Fox
Marten
49-55
236 274
6
7
8
Pine Marten
Mink
Muskrat
220 265
40-75
28 -30
1
2
3
4
5
6
7
112-120
144-150
59 68
56-68
58-75
15 -18
19 31
30-35
21 30
SI Units
Dog
Cat
Cow
Horse
Pig
Sheep
PCV (hematocrit)
102 L/L
3557(2534)
3045(2434)
2446
2743
3643
(2635)
2745
Hemoglobin (Hgb)
g/dL
10 g/L
1219
1015
815
1016
913
9--15
106/L
1012g/L
5.07.9
5.010.0
5.010.0
6.010.4
57
915
Reticulocytes
01.0
00.6
012
Mean corpuscular
volume
fL
fL
6677
3955
4060
3749
5262
2840
Mean corpuscular
Hgb
Pg
pg
21.026.2
1317
1117
13.7
18.2
1724
812
Mean corpuscular
Hgb concentration
g/dL
10 g/L
32.036.3
3036
3036
35.3
39.3
2934
3134
Platelets
103/L
109/L
211621
300800
100800
117256
200500
25075
10 /L
10 /L
5.014.1
5.519.5
4.012.0
5.612.1
1122
412
Neutrophils
5885
4564
1533
5270
2070
1050
103/L
109/L
2.912.0
2.512.5
0.64.0
2.98.5
215
0.76.0
03
02
02
01
04
(segmented)
Neutrophils
(band)
Lymphocytes
Monocytes
10 /L
10 /L
00.45
00.3
00.1
00.1
00.8
821
2736
6263
2142
3575
4075
103/L
109/L
0.42.9
1.57.0
2.57.5
1.25.1
3.816.5
29
210
05
08
06
010
06
Eosinophils
Basophils
10 /L
10 /L
0.11.4
00.9
00.9
00.7
01
00.75
09
04
020
07
015
010
103/L
109/L
01.3
00.8
02.4
00.8
01.5
01
01
01
02
02
03
03
103/L
109/L
00.1
00.2
00.2
00.3
00.5
00.3
u/L
u/L
u/L
u/L
u/L
u/L
mEq/L
mg/dL
mg/dL
mEq/L
mg/dL
1114
1315
52368
1.09.7
0236
3.17.6
1724
00.3
9.111.7
110124
135278
Cat
2597
5501,458
045
738
69214
1.812
58120
2.46.1
1724
00.1
8.711.7
115130
71156
Cow
6.935
4198
18153
60125
0350
617.4
309938
4.315.3
2030
01.6
8.011.4
99107
62193
Horse
2.721
47188
70227
160412
60330
632
112456
18
2430
03.2
10.213.4
98109
71142
Pig
2247
4488
41176
1555
66489
3152
160425
0.54.9
1827
00.5
9.311.5
97106
81134
Sheep
1544
140270
Goat
1552
27156
49123
7.7101
2044
83476
3.521
2027
00.5
9.311.7
101113
4490
61283
66230
1648
2050
79265
9.321
0.10.2
9.011.6
100112
65136
Creatinine
Glucose
Magnesium
Phosphorus
Potassium
Protein
Albumin
Globulin
Sodium
Urea nitrogen
mg/dL
mg/dL
mg/dL
mg/dL
mEq/L
g/dL
g/dL
g/dL
mEq/L
mg/dL
0.51.7
76119
1.62.4
2.95.3
3.95.1
5.47.5
2.33.1
2.44.4
142152
828
0.92.2
60120
1.72.6
3.06.1
3.76.1
6.07.9
2.83.9
2.65.1
146156
1934
0.52.2
40100
1.52.9
5.68.0
3.64.9
6.77.5
2.53.8
3.03.5
136144
1025
0.42.2
62134
1.42.3
1.54.7
2.94.6
5.67.6
2.64.1
2.64.0
128142
1127
0.82.3
66116
2.33.5
5.59.3
4.46.5
5.88.3
2.34.0
3.96.0
139153
8.225
0.92.0
4481
2.02.7
4.07.3
4.36.3
5.97.8
2.73.7
3.25.0
142160
1026
0.71.5
4876
2.12.9
3.79.7
3.85.7
6.17.5
2.33.6
2.74.4
137152
13.26
CHAPTER 16
DRUSGS AND DOSAGE
Please copy and paste the pages from no.96 to no.100