AxioVision

( 4.7.2, 2008)

Carl Zeiss
Imaging Associates Ltd.
www.imas.co.uk

 2009 Carl Zeiss

Carl Zeiss MicroImaging GmbH
07740 Jena, Deutschland
BioSciences | Industrial | Standort Gttingen
Postfach 4041, 37030 Gttingen, Deutschland
: +49 (0) 551 5060 660
: +49 (0) 551 5060 464
: micro@zeiss.de
www.zeiss.de/axiovision


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AxioVision. .
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Carl Zeiss.


1 AxioVision............................................................................5
............................................................................................................... 7
2 AxioCam.........................................................8
2.1 ............................................................................................................................... 8
......................................................................................................................... 10
....................................................................................................................... 10
( )..............................................12
................................................................................................... 12
2.2 AxioCam..................................................................................................... 13
..............................................................................................13
.................................................................................................................................. 13
...................................................................................13
.................................................................................................14
.......................................................................................................... 14
.................................................................................................... 15
................................................................................................................. 15
3 .15
4 ...........................................................................18
......................................................................................................... 18
...............................................................................18
.......................................................................................20
.............................................................................22
5 , ....................................................................23
6 .........................................................................25
...........................................................................27
..................................................................................27
7 .............................................................................................................................. 28
8 .............................................................29
9 ........................................................................................... 32
10 ..................................................................................................................... 33
12 ().................................................38
12.1 .....................................................................................40
12.2 Z-............................................................................................................................. 42
12.3 ........................................................................................................ 44
12.4 ......................................................................................................... 45
13 ...............................45
14 ........................................49
15 ............................................................................................................................. 50

1 AxioVision
AxioVision ,
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Microsoft Windows Carl Zeiss Vision
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Toolbar
Document area
(Images, Forms,
Reports)
Workflow
Workarea

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5

 AxioVision , , Options
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AxioVision.

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2
AxioCam
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Acquisition ()
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2.1
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Auto Snap ( ) ,
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2.2 AxioCam

Frame .
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General ()
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General.
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Enable,
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Processing ().
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AxioCam ICc.

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 Save () Save As ( ) File.

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Documents ( ) . My
Documents, " AxioVision" .
ZVI. ZVI,
AxioVision (*.tif),
Microsoft Windows (*.bmp) JPEG (*.jpg)
JPEG.
ZVI TIFF Save
As. .
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: TIFF ,
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(TIFF, Bitmap JPEG), Burn-in
annotations ( ), Apply display mapping (
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AxioVision.
. Browser () AxioVision.
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 Gallery () ,
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: F6,
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4
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Zoom In / Zoom Out ( / ) ( F7 F8).
Auto Zoom ( ):
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Normal View ( ):
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Full Screen ( ): ESC,
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Navigator ().
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Navigator, ,
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Properties (), Properties, Display
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(RGB).
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View
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Extended Settings ( ),
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Z-, Create Z-Stack image
( Z-).
,
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Create Time
lapse image (
).

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Gallery ()
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DAPI, FITC TRTIC

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Channel Color (
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DAPI
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Z-,
Z-Stack,


z.
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Shift

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Ctrl ,
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( 14).

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Ctrl
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z, , Shift
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z.

z. Extract
Selections ( ),

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Create Image (
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ZVI. ,
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ZVI.
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folder ( ),
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Mode ()
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Generate merged images (
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.
File Type ( )
.
Convert to 8 bit ( 8-) (
*.tif),
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Gray Scale ( )
-.
Apply display mappings (
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( 4).
Burn in Annotations ( )
.
Start (), .
: , File, Export
Batch (). Add files type ( ),
, , Run batch
( ).
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AxioVision , 3,
Open Image ( ) File.
ZVI.
( Z-), 13.

6
AxioVision. -,

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Scaling () (Workarea).

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(
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Single distance
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Properties
() Attributes ().
Scale bar (
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optovar / tube, , ,
optovar

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AxioVision MTB2004
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MTB2004 , AxioVision ,
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, Measure
() Menu ().

7
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Annotations () (
Annotations () 8).
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Properties () Attributes () (
6: ).
: Undo () Redo
() Edit (),
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: ZVI,

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5.
Frequent Annotations ( ):

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8
AxioVision, ,
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Tools () My AxioVision ( AxioVision).

Workflow ( ).
New Workflow ( )
.
Image Capture ( )
.
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Add ().
:
Live () Acquisition ()
Snap () Acquisition ()
Navigator () View / Windows ( / )
Scale bar ( ) Annotations ()
Save () File ()
Move Up /
Move Down ( / ).

 ,
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Workflow.
, 9
.
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Keyboard () My
AxioVision.



.
Tools Options,
Naming ( ).
.

(, 2 = 01 99)
FormatID ( ), %U
Microsoft Windows.

Tools Options,
Storage
().

Autosave
() (
Microsoft Windows,
).
Tools Options,
Acquisition ().

.
Auto save (),

, Storage (),
, Naming (
).
Carl Zeiss Vision Image (*.zvi),

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Snap (),
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9
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AxioVision ,
My Documents
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My Documents All Users ( ),
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Tools Options, Folders
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Folders info ( ).

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Import / Export
Configuration ( /
) Tools.
Export ()
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Next ().
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() .
: (Save to Zip file
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10
Carl Zeiss
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Microscope ()
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Microscope
Microscope
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Settings
Editor ( )
Tools.

FITC.
New (),

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Edit
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( 8) .

11
AxioVision.
, Measure
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Interactive Measurement (
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Configure Tools ( ...)
AxioVision
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Configure Tools
.
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, Draw Features ( )
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 , Measure (), Interactive

Measurement ( ), Load Tools ( ).

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Measure (), Interactive Measurement (
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AxioVision

 , Outline spline (
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Outline spline

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OK, .
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Properties (), Measurement (),
.

(, Excel).
.
Area

Center X

Center Y

Perim

BoundWidth

BoundHeight

Diameter

Radius

DensMeanR

DensMeanG

DensMeanB

DensStrR

DensStrG

DensStrB

.
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Properties (),
Measurement ().

12
()
AxioVision - ,
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Z-: .

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ZVI ( 5),
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Mark&Find.

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Multidimentional Acquisition (
) Workarea;
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Experiment ()
, C, Z, T
(Multichannel), Z (Z-Stack) (Time Lapse) .
.

Experiment_1, Experiment_2
.
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(Multichannel), Z- (Z-Stack)
(Time Lapse).

12.1
,
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Z- (Z-Stack)
(Time Lapse).
C (),
.
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,
(1).

DAPI .
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.
DAPI, .
, My_DAPI.
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DAPI_Narrow DAPI_Wide.
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.

(Exposure)
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(Auto) .
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Z-,
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(Fixed) Time (). ,
, Measurement ().
(Camera) ,
( 2),

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DAPI , ,
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(Autofocus),
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: Go (),
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, (Save to pool),
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Duplicate (), My_DAPI.
, , FITC.
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. Extended Parameters ( ),
Extended Channel Parameters ( ),
.

My_DAPI,
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Channel pool ( ),
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Add to Experiment (
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Experiment ().
Save () ,
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Load ().
: ReUse ( )
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Hardware Settings (
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, , Start (),
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Z-.
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Z-.
Z
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3, . ,
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12.2 Z-
Z, Z-.
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.
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Center ().
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.

Center,
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15 0,5 . ,
8, 7 7
.
Z-Stack navigation
( Z-) ,
.
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Start / Stop ( / ).

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Start () Stop ().
' ', ' ',
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: Z-,
AxioVision z 1,
z 2 . ,
z. All Channels per Slice
( ), AxioVision z,
z, .
z, .

 Optimal distance ( )
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(Nyquist criteria),
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AxioVision , .
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AxioVision Deconvolution (
AxioVision) ,
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" "
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12.3
T, .
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10 , 15 .
135
( t=0).
Cycles (),
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" "
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12.4
Experiment ()
.

.
;
Z- , ,
Center () Start / Stop ( / ) .
Z-
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.
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.

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.

13

AxioVision.
,
5, Open ()
File () ( Open Image ( )).
.
File Import to ZVI ( ZVI).

, Z- .
1:
(Setup Image Dimensions)
,
( , Z-
).
. Scale
lateral (X) ( (X))
/ .
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y, ,
Different Y scaling (
Y).
/ ,

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 .
Next ().

 2: (Channel Setup)
8 ,
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Add channel ( ) Next
.

3: Z- (Z-Stack parameters)
.
Z-.
Extended parameters (
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4: T (T Parameter Setup)

,
.
5: (Compose Images) ,
.
. , Get Selected
( ). .
Consistency (), ,
. ,
, Preview ( ).

Finish () , :

 , ZVI Save
() ( Save as ( )) File ().

14

, Z-
,
(, Z-
). (Image Editor) Edit ()
Change Image Dimensions ( )
Change Image Dimensions (
).
( 4).
1: (Select action) ,
(Keep)
(Remove), .
-

2: (Channel Selection)
,
.
3: (Reduce
pixel resolution)
,
.
X
.

4: Z- (Z-Stack selection)
Z-.
, Enable
this action ( ).
z, From
() To () ,
, Get player
position ( ),
() .
""
(,
/).
5: (Time Point Selection)
. , , Enable this action
( ). Z-.
Preview ( ) / Finish ().
Save () (
Save as ( )) File ().

15

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(, ),
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= 256 , 12 = 4096 , 14 = 16384
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