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AxioVision

( 4.7.2, 2008)

Carl Zeiss
Imaging Associates Ltd.
www.imas.co.uk

2009 Carl Zeiss


Carl Zeiss MicroImaging GmbH
07740 Jena, Deutschland
BioSciences | Industrial | Standort Gttingen
Postfach 4041, 37030 Gttingen, Deutschland
: +49 (0) 551 5060 660
: +49 (0) 551 5060 464
: micro@zeiss.de
www.zeiss.de/axiovision


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AxioVision. .
AxioVision
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. .

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AxioVision ,
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.
AxioCam.
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.
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Carl Zeiss.


1 AxioVision............................................................................5
............................................................................................................... 7
2 AxioCam.........................................................8
2.1 ............................................................................................................................... 8
......................................................................................................................... 10
....................................................................................................................... 10
( )..............................................12
................................................................................................... 12
2.2 AxioCam..................................................................................................... 13
..............................................................................................13
.................................................................................................................................. 13
...................................................................................13
.................................................................................................14
.......................................................................................................... 14
.................................................................................................... 15
................................................................................................................. 15
3 .15
4 ...........................................................................18
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5 , ....................................................................23
6 .........................................................................25
...........................................................................27
..................................................................................27
7 .............................................................................................................................. 28
8 .............................................................29
9 ........................................................................................... 32
10 ..................................................................................................................... 33
12 ().................................................38
12.1 .....................................................................................40
12.2 Z-............................................................................................................................. 42
12.3 ........................................................................................................ 44
12.4 ......................................................................................................... 45
13 ...............................45
14 ........................................49
15 ............................................................................................................................. 50

1 AxioVision
AxioVision ,
. AxioVision ,
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Microsoft Windows Carl Zeiss Vision
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Toolbar
Document area
(Images, Forms,
Reports)
Workflow
Workarea



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5

AxioVision , , Options
() Tools () Reset Window Layout ( ),
AxioVision.

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Workarea ( ):
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Document area ( ):
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2
AxioCam
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Acquisition ()
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AxioCam .

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FireWire, , , AxioCam HRc HRm Rev. 1, 2 AxioCam
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2.1
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AxioCamHR.

(Live) ,
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AxioCam
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Auto Snap ( ) ,
Measure (),
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( fast () medium
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AxioCam .


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Frame
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Close after Snap
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2.2 AxioCam

Frame .
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(2x2, 3x3 ..) .

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Refresh Overview ( ),
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General ()
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Shading Correction
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Enable (),

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General.
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Enable,
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Processing ().
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: Carl Zeiss
AxioCam ICc.

3
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DVD, ,
Open Image ( ) File (). ,
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, Info View (
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Info View , .
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Save () Save As ( ) File.


, , , My
Documents ( ) . My
Documents, " AxioVision" .
ZVI. ZVI,
AxioVision (*.tif),
Microsoft Windows (*.bmp) JPEG (*.jpg)
JPEG.
ZVI TIFF Save
As. .
. BMP .
: TIFF ,
.
(TIFF, Bitmap JPEG), Burn-in
annotations ( ), Apply display mapping (
) Convert to 8-bit ( 8-).
5.
: ,
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AxioVision.
. Browser () AxioVision.
AxioVision , .
, , Load
().

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Change View ( ) ,
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; Refresh (),
.

Gallery () ,
.
: F6,
.

4
.

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:
Zoom In / Zoom Out ( / ) ( F7 F8).
Auto Zoom ( ):
.
Normal View ( ):
( ),
.
Full Screen ( ): ESC,
.

Navigator ().
, , .
Navigator, ,
.


.
,
, AxioCam.

, .
Properties (), Properties, Display
().
, .
(RGB).
,
.
Linear () .

Min / Max ( / ),
.
: Best Fit ( ) Min / Max,
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Properties, .
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12 .
View
(). View Windows () Spliter Display (
). Gallery,
. Synchronize
(), .

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Extended Settings ( ),
,
. Z-,
Z-, Create Z-Stack image
( Z-).
,
,
Create Time
lapse image (
).

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.


Gallery ()
.
DAPI, FITC TRTIC

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Channel Color (
),
,
DAPI
.


Z-,
Z-Stack,


z.
,
, Time Lapse,

.

Shift

.
,

.

Ctrl ,
,

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( 14).

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Ctrl
. Z, ,
z, , Shift
,
z.

z. Extract
Selections ( ),

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Create Image (
)
.

5 ,
ZVI. ,
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ZVI.
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Save As ( ) File () . ,
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ZVI .
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, ( , ), Export
() File. ,
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(Base name)
(Save in)
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. Create project
folder ( ),
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Mode ()
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z .
Generate merged images (
)

.
File Type ( )
.
Convert to 8 bit ( 8-) (
*.tif),
8 TIFF.
Gray Scale ( )
-.
Apply display mappings (
)
( 4).
Burn in Annotations ( )
.
Start (), .
: , File, Export
Batch (). Add files type ( ),
, , Run batch
( ).
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AxioVision , 3,
Open Image ( ) File.
ZVI.
( Z-), 13.

6
AxioVision. -,

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Scaling () (Workarea).

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(
1300x1000 ).
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Advanced (), New
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Single distance
( ).

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, ( x y).
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( ).

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(
9).
: x y ,
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.
.


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Properties
() Attributes ().
Scale bar (
)

.

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:
optovar / tube, , ,
optovar

()

AxioVision MTB2004
.

MTB2004 , AxioVision ,
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.
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, Measure
() Menu ().

7
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Annotations () (
Annotations () 8).
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Properties () Attributes () (
6: ).
: Undo () Redo
() Edit (),
.
: ZVI,

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5.
Frequent Annotations ( ):

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8
AxioVision, ,
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AxioVision , ,
, Microsoft Windows.
, AxioVision ,
. - ,
, AxioVision ,
.

Tools () My AxioVision ( AxioVision).


Workflow ( ).
New Workflow ( )
.
Image Capture ( )
.
,
Add ().
:
Live () Acquisition ()
Snap () Acquisition ()
Navigator () View / Windows ( / )
Scale bar ( ) Annotations ()
Save () File ()
Move Up /
Move Down ( / ).

,
,
Workflow.
, 9
.
:
.

Keyboard () My
AxioVision.



.
Tools Options,
Naming ( ).
.

(, 2 = 01 99)
FormatID ( ), %U
Microsoft Windows.

Tools Options,
Storage
().

Autosave
() (
Microsoft Windows,
).
Tools Options,
Acquisition ().

.
Auto save (),

, Storage (),
, Naming (
).
Carl Zeiss Vision Image (*.zvi),

.

Snap (),
.

9
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, , , CDROM, ZIP . ZVI,

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AxioVision ,
My Documents
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Microsoft Windows
My Documents All Users ( ),
.
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Tools Options, Folders
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(
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(
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,

Folders info ( ).

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Import / Export
Configuration ( /
) Tools.
Export ()
.

Next ().
.
,
, Import
() .
: (Save to Zip file
( ))
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10
Carl Zeiss
, AxioVision,
, .

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.
:
Microscope ()
.

Microscope
Microscope
.

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:
Settings
Editor ( )
Tools.

FITC.
New (),

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Edit
()


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TRITC,
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, Apply () ( Tools, Settings,
User (, , )), .
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( 8) .

11
AxioVision.
, Measure
(). .
.
Interactive Measurement (
) Measure,
Configure Tools ( ...)
AxioVision
, , ,
.
Configure Tools
.
,

, Draw Features ( )
.
,
.

, Measure (), Interactive


Measurement ( ), Load Tools ( ).

,
. OK.
Measure (), Interactive Measurement (
), Start Measurement ( ).
, .


AxioVision

, Outline spline (
). , .

Outline spline

, .
OK, .
.
,
Properties (), Measurement (),
.


(, Excel).
.
Area

Center X

Center Y

Perim

BoundWidth

BoundHeight

Diameter

Radius

DensMeanR

DensMeanG

DensMeanB

DensStrR

DensStrG

DensStrB

.
, ,
Properties (),
Measurement ().

12
()
AxioVision - ,
.
:
: ,
, 32 . ,

( -) .
Z-: .

.

,
,
Z-.
: , (Inside4D)
, AxioVision,
.
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.
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,
.
ZVI ( 5),
: ,
Mark&Find.

.
.
Multidimentional Acquisition (
) Workarea;
, .
Experiment ()
, C, Z, T
(Multichannel), Z (Z-Stack) (Time Lapse) .
.

Experiment_1, Experiment_2
.
,
, ,
(Multichannel), Z- (Z-Stack)
(Time Lapse).

12.1
,
; ,
Z- (Z-Stack)
(Time Lapse).
C (),
.
: ,
.
,
(1).

DAPI .
,
.
DAPI, .
, My_DAPI.
, ,
, DAPI,
DAPI_Narrow DAPI_Wide.
: , AxioVision
, ,
.

(Exposure)
.
:
(Auto) .
,
,
,
.
: ,
.
Z-,
.
(Fixed) Time (). ,
, Measurement ().
(Camera) ,
( 2),

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( 10). ,
, ,
DAPI , ,
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(Autofocus),
.
.
: Go (),
.


, (Save to pool),
.
Duplicate (), My_DAPI.
, , FITC.
: .
. ,
.

, ,
. Extended Parameters ( ),
Extended Channel Parameters ( ),
.

My_DAPI,
. ,
,


.

, ,
, Save to pool (
),
.
Channel pool ( ),
,

Add to Experiment (
).

,
,

.

Experiment ().
Save () ,
, DAPI-FITC.

Load ().
: ReUse ( )
, .

, , ,
.
Hardware Settings (
)

, , Start (),
.

, (,
My_DAPI), DAPI Snap
().
.
:
.
,
.

,
.

Z-.
,
Z-.
Z
.
, , ,
3, . ,
.
,
. ,
, 4.

12.2 Z-
Z, Z-.
:
.
;
Center ().
;
.

Center,
.
,
, .
15 0,5 . ,
8, 7 7
.
Z-Stack navigation
( Z-) ,
.
: ,
.


Start / Stop ( / ).

,
Start () Stop ().
' ', ' ',
,
,
.
: Z-,
AxioVision z 1,
z 2 . ,
z. All Channels per Slice
( ), AxioVision z,
z, .
z, .

Optimal distance ( )
, z , .
(Nyquist criteria),
, .
.
, ,
AxioVision , .
.
, ,
, ,
, . ,
AxioVision Deconvolution (
AxioVision) ,
.
" "
, .

12.3
T, .
: .
, ,
10 , 15 .
135
( t=0).
Cycles (),
,
.
:
.
, Z-
, ,
, ,
.
" "
, .

12.4
Experiment ()
.

.
;
Z- , ,
Center () Start / Stop ( / ) .
Z-
, .
.
,
.

, ,
,
.

13

AxioVision.
,
5, Open ()
File () ( Open Image ( )).
.
File Import to ZVI ( ZVI).

, Z- .
1:
(Setup Image Dimensions)
,
( , Z-
).
. Scale
lateral (X) ( (X))
/ .
,
y, ,
Different Y scaling (
Y).
/ ,

,

.
Next ().

2: (Channel Setup)
8 ,
, .

Add channel ( ) Next
.

3: Z- (Z-Stack parameters)
.
Z-.
Extended parameters (
), ,
. ,
,
, ,
, .
4: T (T Parameter Setup)

,
.
5: (Compose Images) ,
.
. , Get Selected
( ). .
Consistency (), ,
. ,
, Preview ( ).

Finish () , :

, ZVI Save
() ( Save as ( )) File ().

14

, Z-
,
(, Z-
). (Image Editor) Edit ()
Change Image Dimensions ( )
Change Image Dimensions (
).
( 4).
1: (Select action) ,
(Keep)
(Remove), .
-

2: (Channel Selection)
,
.
3: (Reduce
pixel resolution)
,
.
X
.

4: Z- (Z-Stack selection)
Z-.
, Enable
this action ( ).
z, From
() To () ,
, Get player
position ( ),
() .
""
(,
/).
5: (Time Point Selection)
. , , Enable this action
( ). Z-.
Preview ( ) / Finish ().
Save () (
Save as ( )) File ().

15

, .

(, ),
.

,
.

, 8
= 256 , 12 = 4096 , 14 = 16384
.

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.

Microsoft Windows,
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1,0, .
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( Y)
( X).

(
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Sync

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(
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G, B,
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