12/26/15

Entamoeba histolytica

Entamoeba histolytica may be observed in the

feces in two morphology stages (necessary for
diagnostic importance) :
trophozoite
cyst
This specimen are stained in Trichrome

Diagnosis :
histolytica forms in feces, abscess
ulcer
immunological reactions
2
12/26/15

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Entamoeba histolytica
Vegetatif form

ectoplasma

nuclei

endoplasm

RBC

12/26/15

Size : 20-40 micron

One Entamoebic
nucleus
Contain red blood
cells
Endoplasm is
granular
Ectoplasm acts as a
pseudopodia
4

Entamoeba histolytica
Vegetatif form

12/26/15

Size : 20-40 micron

One Entamoebic
nucleus
Contain red blood
cells
Endoplasm is
granular
Ectoplasm acts as a
pseudopodia
5

Entamoeba histolytica
cyst form

12/26/15

Entamoeba histolytica
Cyst form

nuclei

12/26/15

Size : 20-40 micron

Four entamoebic
nuclei (mature
cyst)
Ectoplasm is
granular
7

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Entamoeba coli
vegetative form

12/26/15

Size : 15-30 micron

(rbc : 7 micron)
Entamoeba nucleus
Vacuole Endoplasm
Ectoplasm unclear

Entamoeba coli
Vegetative form

12/26/15

10

Entamoeba coli
cyst form

12/26/15

Size : 15-22 micron

Shape : rounded
Entamoebic nucleus
Contain : 2 nuclei
The position of
nucleus are
separated by vacuole
glikogen

11

Entamoeba coli
mature cyst form

Size : 15-22 micron

Shape : rounded
(oval)
Entamoebic nucleus

Contain : 8 nuclei

12/26/15

12

Giardia lamblia

Giardia lamblia may be observed in the

feces in two morphology stages
(necessary for diagnostic importance) :
trophozoite
cyst
This specimen are stained in Trichrome

Diagnosis :
vegetative forms in diarrheic feces /
duodenal fluid
13
cyst forms in feces
12/26/15

Giardia lamblia
Vegetative form

sucker

nuclei

Parabasal
bodies

axostyles

12/26/15

Size : 14 micron
The shape looks like jambu
monyet (bilateral
symmetrical)
Two nuclei
One sucker
Two axostyles
Two blepharoplasts and four
pairs of flagella between
the anterior and posterior
lateral pairs (doesnt show)
Two parabasal bodies

14

Giardia lamblia
Cyst form

nuclei

Cyst wall

12/26/15

Parabasal
bodies

Ellipsoidal shape
Size : 10 - 14 micron
Two four nuclei
Cyst wall
Two blepharoplasts
Two parabasal bodies

15

Balantidium coli
Vegetative form
Oval shape
Size : 60 micron
Nuclei :
Macronucleus
Micronucleus
(disappear)
Vacuole
Cilia can be seen
vaguely

12/26/15

16

Balantidium coli
Cyst form
Oval shape
Size : 60 micron
Nuclei :
Macronucleus
Cilia cant be seen

12/26/15

17

Cryptosporidium parvum
oocyst

oocyst

12/26/15

very small 10 micron

(4-5 micrometers in
diameter)
Excystment
Mature oocysts contain 4
sporozooites
Oocysts appear as bright
pink to red
containing some dark
granules

18

Cyclospora cayetanensis
oocyst

12/26/15

Size : 15 30 mikron
Transparent cyst wall
Mature oocysts contain 4
sporozooites
Oocysts appear as bright pink
to red
Called giant
Cryptosporidium

19

Cyclospora cayetanensis
oocyst

12/26/15

20

Isospora belli
oocyst
Oval shape red color
size 25 - 33
oocyst contain 2 sporocyst
sporocyst contain
4 sporozoit

12/26/15

21

Blastocystis hominis

nuclei

12/26/15

Oval
Size : 5-30 micron
Two four peripher
nuclei
Cyst wall

22

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23

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24

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25

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26

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27

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28

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29