Manual PocH-100iVDiff Ingles

For Veterinary Use
AUTOMATED HEMATOLOGY ANALYZER
pocH-100i V Diff
Instructions for Use
CHAPTER1:
Introduction
CHAPTER2: Safety Information

CHAPTER3: Design and Function
CHAPTER4: Reagents
CHAPTER5:
Initial Operation
CHAPTER6: Operation
CHAPTER7:
Sample Analysis
CHAPTER8:
Display and Output of Analysis Results
CHAPTER9:
Output
CHAPTER10: Quality Control

CHAPTER11: Calibration
CHAPTER12: Instrument Setup
CHAPTER13: Cleaning and Maintenance
CHAPTER14: Troubleshooting
CHAPTER15: Technical Information
CHAPTER16: Warranty
CHAPTER17: Glossary
CHAPTER18: Appendix
CHAPTER19: GNU GENERAL PUBLIC LICENSE
CHAPTER20: Index
SYSMEX CORPORATION
KOBE, JAPAN
Copyright 2005-2006 by SYSMEX CORPORATION
All rights reserved. No part of this Instructions for Use may be
reproduced in any form or by any means whatsoever without
prior written permission of SYSMEX CORPORATION.
Code No. 461-2406-6

PRINTED IN JAPAN
Date of last revision: October 2006
Software Version: 00-14 and onwards
Revised March 2006
Table of Contents
1.
Introduction ......................................................... 1-1
1.1
1.2
1.3
Safety information in this manual ........................................... 1-3

Names .................................................................................... 1-4
Abbreviations and units used throughout this manual ............ 1-4
2.
Safety Information ............................................... 2-1
2.1
2.2
2.3
2.4
2.5
2.6
2.7
2.8
2.9
2.10
2.11
2.12
Specified conditions of use ..................................................... 2-1

General information ................................................................ 2-1
Bar code safety ....................................................................... 2-2
Electromagnetic compatibility (EMC) ...................................... 2-2
Installation .............................................................................. 2-2
Avoidance of biohazards ........................................................ 2-2
Handling of reagent ................................................................ 2-3
Control blood .......................................................................... 2-5
Maintenance ........................................................................... 2-5
Disposal of materials .............................................................. 2-5
Warning labels on the instrument ........................................... 2-6
Personnel ............................................................................... 2-8
3.
Design and Function ........................................... 3-1
3.1
3.2
Overview ................................................................................. 3-1

Functional Description ............................................................ 3-5
4.
Reagents .............................................................. 4-1
4.1
4.2
4.3
4.4
4.5
pocH-pack D ........................................................................... 4-1

pocH-pack LVD ...................................................................... 4-2
CELLCLEAN............................................................................ 4-3
EIGHTCHECK-3WP ............................................................... 4-4
Symbols used on the labels .................................................... 4-5
5.
Initial Operation ................................................... 5-1
5.1
5.2
5.3
5.4
5.5
5.6
5.7
5.8
5.9
5.10
Delivery, storage until installation ........................................... 5-1

Before installation ................................................................... 5-1
Preparation of installation location .......................................... 5-4
Removal of packing tape ........................................................ 5-4
Putting the sticker ................................................................... 5-5
Connection of reagents and waste bottle ............................... 5-5
Preparation of printer paper .................................................... 5-6
Peripheral devices .................................................................. 5-7
Connection of power cable ..................................................... 5-8
Basic instrument settings ........................................................ 5-8
Sysmex pocH-100iV Diff Instructions for Use
6.
Operation ..............................................................6-1
6.1
6.2
6.3
6.4
Screen display ........................................................................ 6-1

Menu tree ................................................................................ 6-7
Signal tones ............................................................................ 6-8
Timer functions ....................................................................... 6-8
7.
Sample Analysis ..................................................7-1
7.1
7.2
7.3
7.4
7.5
7.6
7.7
7.8
7.9
7.10
Introduction ............................................................................. 7-1

Flowchart of analysis procedure ............................................. 7-1
Checks prior to operation ........................................................ 7-2
Switching ON .......................................................................... 7-3
Quality control ......................................................................... 7-4
Specimen requirements .......................................................... 7-4
Analysis .................................................................................. 7-5
Display of analysis results .................................................... 7-13
Print and output of analysis results ....................................... 7-13
End of operation (Shutdown) ................................................ 7-14
8.
Display and Output of Analysis Results ............8-1
8.1
8.2
8.3
8.4
8.5
Latest sample ......................................................................... 8-1

Stored data ............................................................................. 8-7
Manual Analysis....................................................................... 8-8
Output ................................................................................... 8-12
Histogram error flags ............................................................ 8-12
9.
Output ...................................................................9-1
10.
Quality Control ...................................................10-1
10.1
10.2
10.3
10.4
10.5
10.6
Control material .................................................................... 10-1

Control methods ................................................................... 10-2
Quality control process flow .................................................. 10-5
Preparations ......................................................................... 10-6
Performing a quality control ................................................ 10-13
Recording QC data ............................................................. 10-20
Calibration ..........................................................11-1
11.1
11.2
11.3
11.4
11.5
Samples used for calibration ................................................ 11-1

Establishing reference values ............................................... 11-2
Automatic calibration ............................................................ 11-2
Manual calibration ................................................................. 11-6
Printing calibration history ..................................................... 11-9
Revised March 2006
11.
12.
Instrument Setup ............................................... 12-1
12.1
12.2
12.3
12.4
General information .............................................................. 12-1

Possible settings ................................................................... 12-3
Password setting .................................................................. 12-9
Print set values ................................................................... 12-12
13.
Cleaning and Maintenance ............................... 13-1
13.1
13.2
13.3
Maintenance schedule .......................................................... 13-1

Check instrument status ....................................................... 13-2
Clean TD (transducer) chambers and
diluted sample lines (Shutdown) ........................................... 13-3
Clean transducer .................................................................. 13-4
Clean waste chamber ........................................................... 13-7
Perform Auto Rinse .............................................................. 13-9
Dispose waste fluid ............................................................. 13-10
Clean the sample tube adapter .......................................... 13-12
Remove a clog from transducer aperture ........................... 13-13
Clean aperture of TD (Transducer) chamber
(Drain TD Chamber) ........................................................... 13-14
Drain reagent ...................................................................... 13-19
Calibrate LCD ..................................................................... 13-21
Setting Sequence ............................................................... 13-22
Replace reagent ................................................................ 13-24
System fuse replacement ................................................... 13-27
Replace thermal printer paper ............................................ 13-28
List of consumables ............................................................ 13-29
13.4
13.5
13.6
13.7
13.8
13.9
13.10
13.11
13.12
13.13
13.14
13.15
13.16
13.17
14.
Troubleshooting ................................................ 14-1
14.1
14.2
14.3
General faults, instrument failure .......................................... 14-2

Error messages .................................................................... 14-3
Error messages, possible causes and
actions to resolve the error ................................................... 14-7
Print error log ...................................................................... 14-15
14.4
Revised August 2006
15.
15.1
15.2
15.3
15.4
15.5
15.6
15.7
15.8
15.9
15.10
15.11
Technical Information ...................................... 15-1

Performance characteristics/Specifications .......................... 15-1
System limitations - interferences ......................................... 15-4
Print formats ......................................................................... 15-5
Connecting the bar code reader ........................................... 15-6
Interface protocol ................................................................ 15-13
Output format for host computer ......................................... 15-13
ASTM communication specifications .................................. 15-33
System of units ................................................................... 15-51
Program card replacement procedure ................................ 15-53
Checking the program version ............................................ 15-54
Functional description ......................................................... 15-55
Warranty .............................................................16-1
17.
Glossary .............................................................17-1
18.
Appendix ............................................................18-1
19.
GNU GENERAL PUBLIC LICENSE ...................19-1
20.
Index ...................................................................20-1
Revised March 2006
16.
Introduction
1. Introduction
The Sysmex pocH-100iV Diff is an automated hematology analyzer for
use in veterinary laboratories, and veterinarian office laboratories.
The pocH-100iV Diff is a compact instrument, which is designed for
easy operation. For each operating step, online help is available to
support the user.
The pocH-100iV Diff performs a reliable analysis of nineteen (19)
parameters.
The analysis results are displayed on the color graphic LCD display. In
addition, three (3) histograms are displayed and/or printed.
Values outside the specified upper and lower limits are marked, for
further analysis and checking.
Analysis results and histograms can be printed out on the built-in thermal
printer.
The instrument can also be connected to a host computer etc., and can be
used as one instrument in an analytical system.
The pocH-100iV Diff is equipped with a rinse cup, and after aspirating a
sample or control blood, the pipette is automatically cleaned. It is not
necessary to wipe the sample pipette (aspirating pipette).
This instrument has been designed to keep acoustic noise generation as
low as possible.
The user can adapt the instrument to his/her needs or existing laboratory
conditions by selecting the appropriate settings.
Before operating this instrument, carefully read this manual.
Keep this manual for further reference. For further information, please
contact your Sysmex service representative.
Note:
Operate the instrument as instructed. Reliability of test
results cannot be guaranteed if there are any deviations
from the instructions in this manual. If the instrument
fails to function properly as a result of either the users
operation not specified in the manual or the users
utilization of a program not specified by Sysmex, the
product warranty would not apply.
Revised March 2006
1-1
Introduction
Contact Address
Manufacturer
SYSMEX CORPORATION
1-5-1 Wakinohama-Kaigandori
Chuo-ku, Kobe 651-0073
JAPAN
Europe
EC REP
SYSMEX EUROPE GmbH

Bornbarch 1
22848 Norderstedt, Germany
Phone: +49 40 5 27 26-0
Fax: +49 40 5 27 26-100
Americas
SYSMEX AMERICA, Inc.

1 Nelson C. White Parkway,
Mundelein, IL 60060 U.S.A
Phone: +1-847-996-4500
Fax: +1-847-996-4505
Asia-Pacific
SYSMEX ASIA PACIFIC PTE

LTD.
2 Woodlands Sector 1,
#01-06 Woodlands Spectrum,
Singapore 738068
Phone: +65-221-3629
Fax: +65-221-3687
Ordering of supplies and replacement parts

If you need to order supplies or replacement parts, please contact your
local Sysmex representative.
Service and maintenance
Revised March 2006
Please contact the Service Department of your local Sysmex

representative.
1-2
Introduction
1.1 Safety information in this manual

Biological risks, Warning!, Caution! and Important! statements are
presented throughout this manual to call attention to important safety and
operational information. Non-compliance with this information
compromises the safety features incorporated in the analyzer.
Biological risks
Indicates the presence of a biohazardous material or
condition.
Warning!
If this sign is ignored and the instrument is operated
incorrectly, a potentially hazardous situation could
result in death or serious injury of an operator, or
grave property damage.
Caution, Hot!
Indicates that the marked item can be hot and should
not be touched.
Caution, risk of danger, electric shock

To identify any internal live terminal of which voltage
exceeds 1 kV.
To identify any inlet terminal which can be connected
with the voltage exceeding 1 kV.
Caution!
If this sign is ignored and the instrument is operated
incorrectly, there is a potentially hazardous situation
which may result in injury of an operator, adverse
effect on the analysis results, or may cause property
damage.
Revised March 2006
Important!
Indicates what you need to know in order to maintain
instrument performance and prevent damage.
Note:
Indicates information which will be useful in operating the
instrument.
1-3
Introduction
1.2 Names
Sysmex, pocH-100iV Diff, pocH-pack and EIGHTCHECK are

registered trademarks of SYSMEX CORPORATION.
CELLCLEAN is a registered trademark of SYSMEX

CORPORATION.
CAPIJECT is a registered trademark of Terumo Corporation.
ETHERNET is a registered trademark of Xerox Corporation.
MICROTAINER is a registered trademark of Becton Dickinson

Company.
SARSTEDT, S-MONOVETTE and MICROVETTE are registered

trademarks of Walter Sarstedt Gerte und Verbrauchsmaterial fr
Medizin und Wissenschaft.
ISBT128 (International Society of Blood Transfusion) is copyrighted

by and used under License Agreement with ICCBBA, Inc.
Linux is a registered trademark of Linus Torvalds.

All other trademarks not explicitly mentioned in this manual belong to
their respective owners. Sysmex does not authorize its use.
1.3 Abbreviations and units used throughout this manual

Abbreviations
CBC
Complete Blood cell Count
LD
Lower Discriminator
LL
Lower Limit
QC
Quality Control
T1
Trough Discriminator 1
T2
Trough Discriminator 2
UD
Upper Discriminator
UL
Upper Limit
dL
deciliter (0.1 liter)
fL
femtoliter (10 -15 liter)
microliter (10-6 liter)
pg
picogram (10 -12 gram)
1-4
Revised March 2006
Unit
Introduction
Analysis parameters
This instrument provides results for the following parameters:
WBC
: Number of white blood cells
RBC
: Number of red blood cells
HGB
: Hemoglobin concentration
HCT
: Hematocrit value: Red blood cell ratio of total

blood volume
MCV
: Mean corpuscular volume
MCH
: Mean corpuscular hemoglobin
MCHC
: Mean corpuscular hemoglobin concentration
PLT
: Number of platelets
LYM%
(Dog/Cat/Cattle/
Horse mode)
OTHR%
(Dog/Cat mode)
: % of small white blood cells to total WBC

They are assumed to be equivalent to
lymphocytes.
: % of middle white blood cells to total WBC
neutrophils, monocytes, and basophils.
OTHR%
: % of middle and large white blood cells to total
(Cattle/Horse mode) cells
They are assumed to be equivalent to WBC
other than lymphocyte.
EO%
(Dog/Cat mode)
: % of large white blood cells to total WBC

eosinophils.
LYM#
(Dog/Cat/Cattle/
Horse mode)
OTHR#
(Dog/Cat mode)
: Absolute number of small white blood cells

lymphocytes.
: Absolute number of middle white blood cells
neutrophils, monocytes, and basophils.
OTHR#
: Absolute number of middle and large white blood
(Cattle/Horse mode) cells
They are assumed to be equivalent to WBC
other than lymphocyte.
Revised May 2006
EO#
(Dog/Cat mode)
: Absolute number of large white blood cells

eosinophils.
W-SCR
: % of small white blood cells to total WBC
(OTHER1-13 mode) (white blood cell-small cell ratio).
1-5
Introduction
W-MCR
: % of middle white blood cells to total WBC
(OTHER1-13 mode) (white blood cell-middle cell ratio)
W-LCR
: % of large white blood cells to total WBC
(OTHER1-13 mode) (white blood cell-large cell ratio).
W-SCC
: Absolute number of small white blood cells
(OTHER1-13 mode) (white blood cell-small cell count).
W-MCC
: Absolute number of middle white blood cells
(OTHER1-13 mode) (white blood cell-middle cell count).
W-LCC
: Absolute number of large white blood cells
(OTHER1-13 mode) (white blood cell-large cell count).
RDW-SD
: Calculated distribution width of red blood cells,

standard deviation
RDW-CV
: Calculated distribution width of red blood cells,

coefficient of variation
: Calculated distribution width of platelets,
standard deviation
MPV
(except Cat mode)
: Mean platelet volume
P-LCR
(except Cat mode)
: Ratio of large platelets (volume exceeding 12 fL)

to the total number of platelets
Revised March 2006
PDW
(except Cat mode)
1-6
Safety Information
2. Safety Information
2.1 Specified conditions of use
The pocH-100iV Diff is a veterinary blood cell counter for screening
purpose, and shall only be used for in vitro analysis of veterinary blood
samples such as dog, cat, cattle and other specific species and artificial
control blood.
2.2 General information

Before operating this instrument, carefully read this manual. Keep
this manual for further reference. Observe all cautionary information
in the manual and on the instrument.
This instrument must only be installed as instructed in this manual.
Keep long hair, fingers and clothing away from rotating parts.
Should the instrument emit unusual odors or smoke, turn the main
switch OFF immediately and unplug the power cable. Using the
instrument any further bears the risk of fire, electric shock or
personnel injury. Contact your Sysmex service representative.
Do not spill the liquids such as blood samples or reagent on the unit.
Do not put metal objects such as staples or paper clips on the
instrument. This could cause a short-circuit. If this happens after all,
turn the main switch OFF immediately, and unplug the power cable.
Contact your Sysmex service representative.
Should the instrument leak fluid, turn OFF the power switch
immediately, and unplug the power cable. Using the instrument any
further bears the risk of short circuit, fire, electrical shock or
personnel injury. Contact your Sysmex service representative for
inspection.
Do not touch the electric circuits inside the instrument, especially

with wet hands, where there is a risk of electric shock.
Never insert the power plug into power sockets other than AC100240 V. Please note that the instrument must be grounded.
Avoid damage to the power cable. Do not place any appliances on

the power cable. Do not pull on the power cord.
Turn the power supply OFF before connecting peripheral devices

(such as a host computer or handheld bar code reader), or inserting
the program card.
Revised March 2006
2-1
Safety Information
2.3 Bar code safety

In identifying samples, maximum integrity of data is required. To avoid
bar code identification mistakes, check digits should be used.
2.4 Electromagnetic compatibility (EMC)

This instrument complies to the following IEC(EN) standards:
IEC61326-1:1997+A1:1998+A2:2000 (EN61326:97+A1)
Equipment for measurement, control and laboratory use- EMCRequirements
EMS (Electro-magnetic susceptibility (= interference radiation))

For this issue the Industrial environment requirements with regards
to immunity are fulfilled.
EMI (Electro-magnetic interference (= resistance to jamming))

For this issue the requirements of class B are fulfilled.
The instrument must be installed in a dry and dust-free location.
Install in places where there are no adverse effects from high

humidity and dust.
It must be protected against splashing water.
Do not expose the instrument to excessive temperature fluctuation

and direct sunlight.
Avoid shock and vibrations.
The installation location must be well ventilated.
Avoid installation near devices causing potential interference, such

as radios, centrifuges, personal computer monitors, wireless
communication equipment or similar devices.
Installation of this instrument in places where chemicals are stored or

hazardous gas may be present is not permitted.
2.5 Installation
2-2
In principle, all parts and surfaces of this instrument must be

regarded as potentially infectious, since this instrument analyzes
specimens.
Use protective garments and gloves when operating, maintaining, or

servicing the unit is strongly recommended. Use only specified tools
and parts. After completion of work, wash hands with disinfectant.
Never touch waste, or parts having been in contact with waste, with
your bare hands.
Revised March 2006
2.6 Avoidance of biohazards
Safety Information
Should you inadvertently come in contact with potentially infectious

materials or surfaces, immediately rinse skin thoroughly with water,
and follow your hospital or laboratorys prescribed cleaning and
decontamination procedures.
Control blood must be regarded as potentially infectious. Wear

protective garments and gloves when performing quality control.
Read the note documentation and labeling on all reagents.
Avoid direct contact with reagents. Reagents can cause irritation of

the eyes, skin and mucous membranes.
Should you inadvertently come in contact with a reagent, rinse skin

immediately with plenty of water.
At eye contact, rinse at once with plenty of water. See a physician

immediately. Observe the material safety data sheet.
If reagent was swallowed, obtain immediate medical advice. Observe

the material safety data sheet.
Leave the reagent at room temperature (15 - 30C) for at least 24

hours before using.
Observe the markings on the reagent's packings as well as the

information on the package inserts.
Avoid direct contact with reagents. Reagents can cause irritation of

the eyes, skin and mucous membranes.
Should you inadvertently come in contact with reagent, rinse skin

immediately with plenty of water.
At eye contact, rinse at once with plenty of water and obtain

immediate medical advice. Observe the material safety data sheet
(MSDS).
Respecting all first aid steps in your laboratory is essential.
Avoid contact of dust, dirt or bacteria with the reagent especially

when installing new cubes.
Reagents must not be used after their expiration date.
Handle reagents gently to avoid bubbling. Do not shake! Do not use

directly after transportation.
If reagent is spilt, wipe up with a damp cloth.
If reagent was swallowed accidentally, call a doctor immediately!
If reagents are spilt inadvertently near electrical cables or appliances,

there is a risk of electric shock. Switch the instrument off, unplug and
remove the liquid.
Revised March 2006
2.7 Handling of reagent
2-3
Safety Information
CELLCLEAN is a strong alkaline cleaning material. It should not

come in contact with skin or clothing. If it happens nevertheless,
rinse skin or clothing with plenty of water to avoid injury or damage,
respectively.
CELLCLEAN contains sodium hypochlorite. If CELLCLEAN

makes contact with the instrument's surfaces, it will affect the surface
finish and there is danger of corrosion. Immediately wipe up
CELLCLEAN with a damp cloth.
Make sure the reagents used with the instruments are kept level or
below the main unit of the instrument. Do not put reagents on top of
the instrument.
When reagent is to be stored, store the reagent at the specified

temperature for each reagent.
Important!
Strictly follow the measures of first aid in the
laboratory:
Revised March 2006
Acids: e.g. acetic-, sulfuric-, hydrochloric-, nitric- and

phosphoric acid
First aid actions:
after contact with:
Lungs - remove from exposure, rest and keep warm - in
severe cases or if exposure has been great, obtain medical
attention.
Skin - drench the skin copiously with water; - remove
contaminated clothing and wash before reuse;
- in severe cases obtain medical attention;
Blisters or burns should receive medical attention.
Mouth - Wash out the mouth thoroughly with water and
give water to drink together with milk of magnesia or milk.
- Keep patient warm and quiet.
Alkalis: e.g. sodium-, potassium-, ammonium- or calcium
hydroxides.
Lungs - remove from exposure, rest and keep warm - in
severe cases or if exposure has been great, obtain medical
attention.
Skin - drench the skin with plenty of water; - remove
contaminated clothing and wash before reuse;
- in severe cases obtain medical attention;
Mouth - Wash out the mouth thoroughly with water; give
copious water followed by vinegar or 1% acetic acid to
drink or give copious amounts of lemon juice: - obtain
medical attention.
2-4
Safety Information
2.8 Control blood
Do not inject or ingest.
Control blood must always be stored in an upright position irrespective of whether the vial has been opened or is still closed.
Always wear protective garment and gloves for all service and
maintenance work. Use only specified tools and parts. After
completion of work, wash hands with disinfectant. Instrument
surfaces in contact with blood is potentially biohazardous.
When performing out maintenance work, use only the specified tools
and parts. Install only such spare or replacement parts intended for
this equipment.
When cleaning the instrument surface such as the sample position or

the touch panel, use a soft dry cloth, cloth soaked in neutral
detergent then wrung tightly, or soft cloth dampened with
ethanol.
Do not use any organic solvent, acid, or alkaline agent. These will
affect the instrument surface finish and may cause corrosion or
discoloration.
If the instrument is not operated every day, "Blank Error" may occur.
In this case, follow the instructions displayed in the action message
screen.
2.9 Maintenance
2.10 Disposal of materials

Disposal procedures for residual reagents, detergent and all waste must
meet all applicable local regulations.
Revised March 2006
Biological risks
To avoid risks of infections, wear protective garments
and gloves for all cleaning or maintenance work. After
completion of work, disinfect your hands and wash
them afterwards. Otherwise, there is a risk of infection
by pathogens.
2-5
Safety Information
2.11 Warning labels on the instrument
Warning!
This equipment must be Grounded.
To avoid electrical shock, disconnect supply

before servicing.
For the continued protection against risk of fire,

replace only with fuse of the specified type and
current ratings.
Never open the instruments cover on the back

when the power switch is on. Do not open this
cover unless absolutely necessary. There are no
user serviceable parts inside.
PUSH
Push here, and this door will open.
PUSH
SH
PU
Head
Caution, Hot!
The printer head becomes hot. Use caution.
Revised March 2006
Caution!
Static electricity may damage the printer head. Do not
touch with hands.
2-6
Safety Information
:
BR
BR :
Bar code Reader
LAN
Serial Port (HC Connector)
Caution!
Static electricity may damage the electronic circuit
via the connectors on the right side. Do not touch
connector pins with hands.
Turn the main switch OFF before inserting or
removing connectors or program cards.
Caution, risk of danger, electric shock

The LCD inveter becomes the high voltage exceeding
1 kV. Do not touch during power supply on.
Contrast adjustment lever
Caution!
Contact with static electricity at the lower left part at
the front side may result in abnormal instrument
operation.
During operation, do not touch any parts other than
the contrast adjustment lever.
Revised March 2006
Caution!
When opening the detector cover to clean the
transducer aperture, follow the instructions in Section
13.10 Clean aperture of TD (Transducer) chamber
(Drain TD Chamber).
Because there is a risk of electric shock, do not open
this cover for any other purpose.
Detector cover
2-7
Safety Information
Caution!
When opening the right side cover for maintenance,
turn the main switch OFF and disconnect the mains
connection (inlet).
Because there is a risk of electric shock and of
pinching hands by the moving parts, do not open this
cover for any other purpose.
2.12 Personnel
Personnel using this instrument must read the Instructions for Use
thoroughly beforehand, and must operate the instrument correctly.
Personnel who have less experience operating the instrument should

receive guidance and assistance from an experienced operator.
If the instrument requires service, consult the Instructions for Use for
troubleshooting information. If additional repair is required, contact
your Sysmex representative.
Revised March 2006
2-8
Design and Function
3. Design and Function

3.1 Overview
Front view (with the front lower panel removed)
(3) Color LCD screen
(4) Contrast adjustment

knob
(1) Pinch valve 1
(2) Pinch valve 2
(5) Sample position
(1) Pinch valve 1

Controls the flow of the waste fluid from the rinse cup.
(2) Pinch valve 2
Starts and stops the flow of waste fluid inside the instrument to the
waste bottle.
(3) Color LCD screen
Displays the sample ID number, analysis results, instrument status,
error messages, and menus.
(4) Contrast adjustment knob
Adjusts the contrast of the LCD screen.
July 2005
(5) Sample position

Holds the adapter containing the sample tube for analysis.
3-1
Design and Function

Right view (with the right side panel opened)
(10) Built-in thermal printer
(1) Main
power switch
(5) Trap chamber
(6) Socket for LAN
(2) Aspirating
pipette
BR
(7) Socket for

bar code reader
(8) Serial port
(3) Rinse cup

(4) Detector
(9) Card slot
(1) Main power switch

Turns ON or OFF of the main power supply.
Important!
Avoid turning this switch ON and OFF repeatedly in
short duration. This will overload the fuse and may
cause a fuse to blow.
(2) Aspirating pipette

Aspirates sample. Sample tube may be open (cap removed).
Warning!
The tip of the pipette is sharp. Do not touch the tip
with fingers.
July 2005
Caution!
DO NOT place a sample tube with a cap made of
plastic resin.Pipette may be permanently damaged.
3-2
Design and Function

(3) Rinse cup
Rinses outside and inside of the pipette with diluent to avoid
sample-to-sample carry-over.
(4) Detector
Consists of RBC transducer, WBC transducer and Hemoglobin
detector.
(5) Trap chamber
Avoids any back-flow of the reagents to the vacuum pump if
malfunction has occurred.
(6) Socket for LAN
Connects an Ethernet connector (RJ45) for the communication with
host computer, etc.
(7) Socket for bar code reader
Connects an optional bar code reader.
(8) Serial port
Connects a 9-pin D-SUB serial interface connector for the
communication with host computer and etc.
(9) Card slot
Inserts a PC card specified by Sysmex.
Caution!
Use only program cards specified by Sysmex.
July 2005
(10)Built-in thermal printer

Prints analysis results, setting values, and other information.
3-3
Design and Function

Rear side
(6) System fuses
(5) AC power supply
(1) Float switch connector for waste bottle

(2) Diluent inlet nipple (blue)
(3) Lyse inlet nipple (purple)
(4) Waste outlet nipple (red)
(1) Float switch connector for waste bottle

Connects the float switch which monitors the waste level in the
bottle.
(2) Diluent inlet nipple
Aspirates diluent.
(3) Lyse inlet nipple
Aspirates lyse reagent.
(4) Waste outlet nipple
Discharges waste through this nipple by the vacuum accumulated in
the waste bottle.
Biological risks
To avoid risks of infections, wear protective garments
and gloves for all cleaning or maintenance work. After
completion of work, disinfect your hands and wash
them afterwards. Otherwise, there is a risk of infection
by pathogens.
July 2005
(5) AC Power supply (inlet)

Supplies the power (AC 100 V-AC 240 V) from the outlet using the
provided power cord.
3-4
Design and Function

(6) System fuses
Protects the system from over-current. (See the list of provided parts
in Section 5.2 Before installation)
Warning!
To avoid electrical shock, disconnect power cord
before servicing.
(The main switch is not the disconnection device.)
replace only with fuse of the specified type and
current ratings.
3.2 Functional Description

Starting
After switching ON the instrument, it performs a Self-Check.
During this check, the internal service counters are checked.
If the Self-Check establishes the need for maintenance, an alarm sounds
and a respective message will appear on the LCD screen.
Background check
After the Self-Check, hydraulics and measuring chambers are rinsed and
a background check is performed.
Sample aspiration
Set the sample tube into the analysis position.
July 2005
By pressing the [RUN] button on the LCD screen, the sample is

aspirated and the analysis starts.
3-5
Design and Function

Analysis
The sample is aspirated with the aspirating pipette. An exact volume is
sent into the mixing chamber and the transducer, together with a
measured volume of the diluent.
All parameters are analyzed in the order of: (1) WBC/HGB and (2) RBC/
PLT using the same transducer.
For WBC/HGB analysis, the WBC/HGB lyse is added to the measuring
chamber for further dilution and hemolysing. The sample RBCs lyse in
the chamber for about ten seconds.
During this reacting period, the red blood cells are dissolved under the
influence of the lysis, hemoglobin emerges and is converted into acid
methemoglobin. The white blood cells remain intact.
The volume and number of the white blood cells (WBC) are determined
by the DC detection method. In the HGB detector, the hemoglobin
concentration is photometrically measured.
For RBC/PLT analysis, the diluted sample is transferred from the mixing
chamber to the transducer, and the volume and blood cell count for red
blood cells and platelets are analyzed by the DC detection method.
An exact volume of sample is aspirated with the aspirating piercer. This
volume of sample is then transferred (together with a defined volume of
diluent) to the mixing chamber and automatically to the transducer.
Parameter calculation
Based on the measured values, the microprocessor calculates the
remaining parameters.
Display
After completion of the analysis, the data is stored and displayed on the
LCD screen.
Output
The analysis results can be printed out on the built-in thermal printer or
transferred to the laboratorys host computer.
Preparations for the next analysis
The diluted sample flow system is rinsed.
July 2005
The sample ID is automatically incremented by 1, if no bar code is used

or another sample ID number entered. The instrument is then ready to
perform the next analysis.
3-6
Reagents
4. Reagents
4.1 pocH-pack D
Intended purpose
Diluent for use in hematology analyzers.
Storage and shelf life after first opening
The expiration date is shown on the outer packaging. Store the pocHpack D at 1 - 30C. If an unopened pocH-pack D is stored at 1 to 30C, it
remains usable for 12 months after the manufacture.
Once opened (connected to the instrument), product stability of the
reagent is a maximum of 60 days.
Any pocH-pack D revealing signs of contamination or instability, as
indicated by cloudiness or color change, should be replaced.
Methodology
The pocH-pack D is a ready-to-use diluent for DC detection and
absorbance analysis of whole blood.
Composition
Sodium Chloride
Boric acid
Sodium Tetraborate
EDTA-2K
6.38 g/L
1.0 g/L
0.2 g/L
0.2 g/L
Ambient temperature
Use the pocH-pack D at 15 - 30C. If analysis is performed at
temperatures exceeding 30C or below 15C, correct analysis results
may not be obtained.
Specimen
Use whole blood sample collected in EDTA anticoagulant.
Additional special equipment
July 2005
The pocH-pack D is solely intended for use with Sysmex reagents and
analyzers.
If other reagents are used, the product performance of Sysmex
instruments cannot be guaranteed.
4-1
Reagents
4.2 pocH-pack LVD

Intended purpose
The pocH-pack LVD is a reagent that lyses RBC for accurate WBC
count and hemoglobin determination.
It is used for WBC volume distribution analysis and hemoglobin
measurement.
The reagent is colorless, transparent and contains no cyanide.
The pocH-pack LVD is intended for use in Sysmex Automated
Hematology Analyzers.
The expiration date is shown on the outer packaging. Store the pocHpack LVD at 2 - 35C. If an unopened pocH-pack LVD is stored at 2 to
35C, it remains usable for 12 months after the manufacture.
Once opened (connected to the instrument), product stability of the
reagent is a maximum of 90 days.
Any pocH-pack LVD displaying any signs of contamination or
instability, as indicated by cloudiness or color change, should be
replaced. Do not use the pocH-pack LVD once frozen.
Methodology
By lysing the red blood cells, pocH-pack LVD allows a white blood cell
count and volume distribution analysis by the DC detection method.
Hemoglobin is released during RBC lysis, and is converted into acid
methemoglobin.
A portion of this diluted sample is transferred automatically to the
hemoglobin detector, where the absorbance of the red pigment is
measured to provide the blood hemoglobin concentration.
Composition
Sodium Chloride
Org.quart. ammoniumsalt
4.0 g/L
3.3 g/L
Ambient temperature
Specimen
Use whole blood sample collected in EDTA anticoagulant.
4-2
Sysmex Corp. pocH-100iV Instructions for Use
Revised May 2006
Use the pocH-pack LVD at 15 - 30C. If analysis is performed at

temperatures exceeding 30C or below 15C, correct analysis results
may not be obtained.
Reagents
The pocH-pack LVD is solely intended for use with Sysmex reagents
and analyzers.
If other reagents are used, the product performance of Sysmex
instruments cannot be guaranteed.
4.3 CELLCLEAN
Intended purpose
Strong alkaline detergent used to remove lyse reagents, cellular residuals
and blood proteins remaining in the hydraulics of the instrument.
Warning and precautions
Warning!
Avoid contact with skin and eyes. In case of skin
contact, flush the area with water.
In case of contact with eyes, rinse immediately with
plenty of water and seek medical advice.
If swallowed, seek medical advice and follow MSDS.
Store CELLCLEAN in a dark place at 15-30C.
Avoid exposure to direct sunlight, if exposed to direct sunlight, the
chlorine component will degrade and the effectiveness of this detergent
will be lost depending upon the time period of exposure. Once opened,
this reagent should be used within 60 days.
Methodology
CELLCLEAN is a detergent used to clean the instrument in order to
remove residuals of lyse reagents, cellular residuals and blood proteins
from the hydraulic systems, transducer, whole blood aspiration piercer
and the Hgb flow cell.
Composition
5.0 %
July 2005
Sodium Hypochlorite
4-3
Reagents
4.4 EIGHTCHECK-3WP
Please see Section 10.1 Control material.
Intended purpose
Control blood for testing the precision and accuracy of automated and
semi-automatic hematology analyzers. Do not use EIGHTCHECK-3WPN, EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H for the
calibration of the system!
The human blood used for the production of EIGHTCHECK-3WP-N,
EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H has been tested
with licensed reagents (Food and Drugs Administration's Bureau of
Biologic, USA) and has been found non-reactive for Hepatitis B Surface
Antigen, Hepatitis C Virus (HCV) and HIV (HIV1+HIV2) Antibody.
However, none of the test methods known today can guarantee that
products based on human blood are not infectious. In keeping with good
laboratory practice, this product should be considered potentially capable
of transmitting infectious disease.
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK3WP-H are to be stored according to the instructions in the package
insert.
When handled in this manner, the unopened product is guaranteed stable
until the expiration date stated on the package.
July 2005
After opening, the product is guaranteed stable for 7 days if returned to

the refrigerator promptly after use.
4-4
Reagents
4.5 Symbols used on the labels
Consult Instructions for Use
Batch code
Use by
Temperature limitation
CE conformity sign as per directive 98/79/EC
Manufacturer
July 2005
Authorized representative in the European

EC REP community
4-5
July 2005
Reagents
4-6
Initial Operation
5. Initial Operation
5.1 Delivery, storage until installation
Make sure the instrument is transported and handled carefully.

Important!
If there is any damage to the packaging, clearly list
the damage conditions on the invoice, and apply your
receipt stamp. Open the package in the presence of a
representative from the transport company. For
information about sending replacement instrument,
Until installation, store the instrument until installation in its package

in a dry location. It must be stored in an upright position.
Keep the packaging, boxes and padding material. They may be

needed in the future if the instrument is to be returned for repair.
5.2 Before installation

Check that there is no damage on the main unit of the pocH-100iV Diff.
Also, check the quantity of provided parts.
List of provided parts for pocH-100iV Diff
No.
Parts No.
Description
Quantity
461-2406-6
Instructions for Use (English)
462-3523-6
Transducer Brush No. 3
Revised March 2006
5-1
Initial Operation
No.
5-2
Parts No.
Description
Quantity
265-4731-5
Power Cord 4622-007-0092 (Europe)
442-3047-7
Sample tube adapter (13mm)

(Color: cream)
442-3045-0
Sample tube adapter (Control blood)

(Color: cream)
033-0861-0
Adapter (for 8 mm diameter)

(Color: purple)
033-0891-1

(Color: green)
033-0871-7

(Color: yellow)
442-3044-6
Adapter
(Color: cream)
10
033-0881-4

(Color: blue)
11
442-3042-9

(Color: grey)
12
462-4846-1
Paper Thermal CL6040
13
423-1776-2
Mini Pipette No.10 (1mL)
14
266-5330-1
Fuse 250V 3.15A H

Time Lag
2
Revised March 2006
Initial Operation
No.
Parts No.
Description
Quantity
15
462-2381-8
Screwdriver (+)
16
462-2390-1
Screwdriver (-)
17
033-0413-9
Container Spout Kit No.7 (pocH-pack LVD)
(Color: orange)
18
023-2441-5
Container Spout Kit No.5 (pocH-pack D)
(Color: blue)
19
033-0041-1
WASTE BOTTLE ASSY POCH

(C1/WITH FSW)
20
367-2170-9
Reagent Tray
21
266-7110-2
Coil Tube SS-10
22
369-5172-1
Sticker
(For Veterinary Use)
Revised March 2006
For Veterinary Use
5-3
Initial Operation
5.3 Preparation of installation location

150 mm
or more
300 mm
150 mm
or more
150 mm
or more
350 mm
460 mm
This instrument must be installed in a dry, dust free location.

The weight of this instrument, together with the reagents, is
approximately 17 kg (37.4 lb). Install onto a table or desk which can
support this load.
Sufficient space for carrying out maintenance or service work must
be provided. For proper cooling, the distance from the right side,
rear, and top panels to walls should be at least 15 cm (6 in).
Avoid installation of the instrument near devices that cause high
frequency interference, such as radio equipment, communication
equipment, etc.
The power supply cable is 1.8 m (5 ft) long. Ensure a suitable outlet
is available nearby. See Section 15.1 Performance characteristics/
Specifications.
For calculating air conditioning requirements, a minimum cooling
capacity of 130 kcal/h is required to offset the heat generated by the
instrument.
When relocating, lift up the bottom of the equipment carefully. And
avoid shock and vibrations.
The instrument dimensions including the reagent are shown in the
table below.
Instrument main unit

+ reagent
Width
(mm/in)
Depth
(mm/in)
Height
(mm/in)
Weight
(kg/lb)
300/11.8
460/18.1
350/13.8
Approx.
17/37.4
Warning!
Do not place anything on the top panel. This may
cause heat build up, and may result in the instrument
failing to operate correctly.
In addition, if fluids are spilt in the instrument, short
circuits and smoking may result.
5.4 Removal of packing tape
Revised March 2006
1. Remove all of the packing tape.
5-4
Initial Operation
5.5 Putting the sticker

1. Put the provided sticker on the front cover.
For Veterinary Use
5.6 Connection of reagents and waste bottle

Waste bottle
pocH-pack
D
1. Set the reagents and waste bottle in the provided reagent tray, then
remove the caps.
The cap that is attached to the reagent bottle will be needed later
when disposing the waste bottle.
pocH-pack LVD
Reagent tray
Caution!
Leave the reagent at room temperature (15 30C) for at least 24 hours before use. Or a
correct analysis result will not be obtained.
Use the reagent at 15 - 30C. When analyzed with

the reagent of a temperature higher than 30C or
lower than 15C, a correct analysis result will not
be obtained.
After unpacking, take care to prevent dirt, dust, or

bacteria from entering.
2. Insert the container spout kits and float switch into the reagent bottles
and waste bottle, respectively. Then apply the caps.
Container
spout kit
Float switch
Waste
bottle
Revised March 2006
pocH-pack
D and
pocH-pack
LVD
Caution!
Do not insert the container spout kit and float
switch into a wrong bottle.
Take care not to touch the tubes that enter the

reagent with your hands, or to allow dust or other
substances to adhere to them. If such substances
adhere to a tube, first wash the substance off with
reagent before attaching the container spout kit.
Failure to do so may prevent correct analysis.
Take care to prevent the reagents from spilling. If

a reagent spills, immediately wipe it off with a
damp cloth or similar material. The reagents may
cause discoloration of some materials.
5-5
Initial Operation
Float switch
connector for
waste bottle
Diluent inlet
nipple (Blue)
3. On the rear side of the instrument connect the tubes of the container
spout kits and the waste outlet nipple as well as the connector at the
float switch to its connection.
Lyse reagent
inlet nipple
(Purple)
Caution!
Do not connect the tubes to the wrong nipples.
4. Using the provided coil tube, lump the tubes together.
Waste outlet
nipple (Red)
5.7 Preparation of printer paper

1. Open the printer paper holder of the built-in thermal printer by
pushing the knob.
Caution, Hot!
Caution!
touch with hands.
Push
2. Remove the tape holding the end of the printer paper, then set the
printer paper into the printer paper holder.
Revised March 2006
Tape holding
5-6
Initial Operation
3. Close the printer paper holder so that the roller catches the paper.
Roller
Important!
Set the paper straight in the correct direction. If
inserted at an angle, the paper may become
jammed.
The printer cover must be closed securely until a
"click" sound is heard. If the cover is not closed
completely, an error will occur.
Thermal head
5.8 Peripheral devices

Caution!
When connecting peripheral devices, this instrument
must be switched off.
The following optional devices can be connected.

Bar code reader (optional)
A bar code reader scans the bar code on the sample collection tube and
automatically inputs the sample number.
Revised March 2006
Important!
The bar code reader is not included as a standard
accessory. Refer to the bar code reader manual for
detailed information on the connection of a bar code
reader, see Section 15.4 Connecting the bar code
reader.
5-7
Initial Operation
5.9 Connection of power cable

Insert the provided power cable into the power connector on the rear of
the instrument. Connect the other end to an AC outlet.
Warning!
Be sure to ground the instrument.
Improper grounding may cause electrical shock.
5.10 Basic instrument settings

Note:
In this chapter, only the settings relevant to the instrument
installation are described. For details on all possible
settings, see Chapter 12. Instrument Setup.
Date and time settings

In order to have analysis results properly identified, it is important to
have the date and time set correctly.
Note:
When time changes to summer or winter time, respectively,
the clock must be corrected accordingly.
Revised March 2006
1. Set the main power switch to position "I" ON.

When the main power switch is turned ON for the first time after
shipment from the factory after forming or the draining operation, the
instrument will automatically aspirate the reagent into the instrument
(priming).
After 11 to 16 minutes, the display indicates Ready.
5-8
Initial Operation
Ready
Menu
Main
ID
2. Press the [Menu] button.

The Menu screen will appear.
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data
Chg.Reag
Calib.
Settings
3. Press the [Settings] button.

The Settings menu screen will appear.
Maint.
Not Ready
Top
Settings
Note:
If a password has been set, the password input screen will
appear. Enter the password. No password is set when the
instrument is shipped; the password input screen will not
appear, until a password is set.
Enter Password.
Not Ready
Top
Settings
Host Output
Date/Time
Built-in Printer
Species
Network
Quality Control
Password Setting
User Information
Print Settings
Revised May 2006
System
4. Press the [Date/Time] button.

The initial Date/Time setting screen will appear.
5-9
Initial Operation
Not Ready
Top
Date/Time
Format
Year
dd/mm/yy
2003
Month
02
Day
03
5. Press the Format display column to select a date display format.

Display format
Example of display (for February 3, 2003)
yy/mm/dd
2003/02/03
mm/dd/yy
02/03/2003
dd/mm/yy
03/02/2003
The underlined format is the initial setting.

Quit
6. Press the Year display column.

The numerical keys dialog for entering year will appear.
7. Enter the year, and press the [Ent.] button.
If an incorrect value was entered, press the [C] button and enter the
correct value again.
8. Enter the month and day in the same way as the year.
Important!
If an incorrect date was entered (e.g. 4/31 or 2/29 in a
year which is not a leap year), a beep sounds.
Reenter the correct date.
Not Ready
Top
Date/Time
Hour
10
Minute
15
9. Press the [] button to bring up the second Date/Time setting

screen.
10. Enter the hour and minute in the same way as when entering the year.
11. Press the [Quit] button to update the settings.
The setting update confirmation message will appear.
Press the [OK] button to update the changed settings.
Press the [Cancel] button to close the dialog and continue to

change settings.
Ent.
Revised March 2006
Quit
* Press the [Top] button on the setting screen.

The setting stop confirmation dialog will appear.
Press the [OK] button to return to the Main screen without
changing the settings.
change settings.
5-10
Initial Operation
Sample collection tube settings
9 mm
Because a S-MONOVETTE (SARSTEDT) tube has a different bottom

shape, the insertion of the pipette needle must be reduced by 9 mm
compared to the other sample collection tubes. For this reason, before
analysis, set the type of sample collection tube to be used.
S-MONOVETTE
(SARSTEDT) sample
collection tube
Standard sample
collection tube
Ready
Menu
Main
ID
Caution!
When S-MONOVETTE (SARSTEDT) is selected,
use S-MONOVETTE EDTA K collection tubes
manufactured by SARSTEDT.
S-MONOVETTE (SARSTEDT) sample collection
tubes cannot be used together with other sample
collection tubes.

1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data
Chg.Reag
Calib.
Settings

Maint.
Not Ready
Top
Settings
Note:
If a password has been set up, the password input screen will
appear. Enter the password. No password is set when the
instrument is shipped; the password input screen will not
appear until a password is set.
Revised May 2006
Enter Password.
5-11
Initial Operation
Not Ready
Top
Settings
System
Host Output
Date/Time
Built-in Printer
Species
Network
Quality Control
Password Setting
User Information
Print Settings
Not Ready
System
Units
Type 2
Language
English
Tube
STANDARD
Volume
Alarm
Top
3. Press the [System] button.

The initial system setup screen will appear.
4. Press the display column of the Tube to select a sample collection

tube.
STANDARD:Standard sample collection tube
SARSTEDT:S-MONOVETTE (SARSTEDT) sample collection tube
5. To update settings, press the [Quit] button.
The store data confirmation message will be displayed.
Press the [OK] button to update the settings.
Type 1
Quit

change settings.
Press the [Top] button on the setting screen.
The setting stop confirmation dialog will appear.
Revised March 2006

change settings.
5-12
Initial Operation
Adjusting the contrast of the LCD screen
Slide the front cover of the instrument to the left to open it. The LCD
contrast can be adjusted with the contrast adjustment knob located on the
right side of the pinch valve. Slide the knob to the left to darken the LCD,
and to the right to brighten it.
Note:
If no operation is performed on the LCD screen for a
certain period of time, the LCD backlight illuminance will
decrease automatically to conserve electric power.
(backlight saving timer function.) Touch any portion on the
LCD screen to return to a normally illuminated LCD
screen.
Revised March 2006
Contrast adjustment knob
5-13
Revised March 2006
Initial Operation
5-14
Operation
6. Operation
6.1 Screen display
To a large extent, the instrument is menu-driven. The LCD screen shows
which functions or submenus are available. Also, the instrument's
operating status and the progress of a measurement, respectively, are
displayed.
Main screen/Menu screen
Ready
After turning the power ON, the Sysmex logo appears, and the startup
operation is performed. Then the Main screen will appear. When the
[Menu] button on the Main screen is pressed, the Menu screen will
appear.
Menu
Main
ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
The menu to select a required function is displayed on the Menu screen.

Auxiliary operations such as instrument maintenance and settings are
performed from this screen.
Top
Menu
Str.Data
Chg.Reag
Calib.
Settings
Press the [Top] button on the upper right of the screen to return to the
Main screen from the Menu screen.
Maint.
System area
(1)(2)(3)(4)
(5)
Ready
Menu
Main
ID
Species (1)Dog
Send a mixed
sample, and
press [RUN].
(6)
The following information is displayed in the system area on the upper

part of the LCD screen.
(1) Instrument status indicator
Displays the status of the instrument. The status display shows four
types of conditions for the instrument: Ready, Aspirating,
Running, and Not Ready.
Ready:
Analysis operation can be started.
RUN
select
Result
Shutdown
Revised May 2006
QC
Aspirating:
Sample is being aspirated.
Running:
Analysis operation is being performed.
Not Ready:
Analysis operation has stopped.

The analysis cannot be started.
6-1
Operation
(2) Screen name
Displays the screen name currently displayed in the Data Processing
Area.
Possible screen names: Main, Result, Menu, Settings, etc.
(3) Remaining Reagent volume indicator
Displays the remaining reagent volume by eleven levels.
Note:
The remaining reagent volume indicator should be used
only as a guide. Depending on the usage method of the
instrument, the remaining reagent volume may not be
correctly displayed.
Caution!
If the remaining reagent is decreased and the
Replace pocH-pack D" or "Replace pocH-pack LVD"
error has occurred, replace the reagent with a new
one.
(4)
(5)
(help) button:
This button blinks only when an error

occurs.
Displays the Action Message screen when
an error occurs, and displays the Error List
screen when two or more errors occur at
the same time.
button: The [Menu] button is indicated when the

Main screen is displayed, and it changes
into the [Top] button when any other
screen than the Main screen is displayed.
Revised March 2006
(6)
(paper feed) button: Executes the paper feed of the built-in

printer. This button is effective on any
screen as long as no dialog window is
displayed.
6-2
Operation
Data processing area
Not Ready
Top
Result
ERROR
WBC
LD
T1
T2
The Data Processing Area at the center of the LCD screen changes
together with the screen contents.
ID 123456789012345 01/01 12:10

[S] [W] [R] [P]
WBC
WL+ 8. 9 x103/uL
LYM%
31. 0 %
8. 5 %
OTHR%
EO%
60. 5 %
LYM#
2. 8 x103/uL
0. 8 x103/uL
OTHR#
30 fL
EO#
5. 3 x103/uL
120 fL
180 fL
3/5
Print
(1)Dog
HC Out
Basic operation area

Ready
Menu
Main
ID
Species (1)Dog
The messages and the buttons are displayed in the Basic Operation area
at the bottom of the LCD screen.
Press the corresponding button to execute a basic menu operation and the
cursor operation.
Set a mixed
sample, and
press [RUN].
RUN
select
QC
Result
Dhutdown
Action message screen

NOT Ready
Top
help
Printer Error
This screen appears when an error has occurred and the

been pressed.
button has
Follow the instructions on this screen to easily perform a recovery

process.
Error occurred on the Built-in

Print. Close the printer cover.
[Retry] Restart to print.
[Cancel] Cancel to print.
ERROR CODE : xxxxx. xxxxx. xxxxx
Cancel
Revised May 2006
Retry
6-3
Operation
Dialog screen
This box prompts the operator for confirmation. Dialogs include the
Error dialog when errors occur, the Confirmation dialog when
settings are changed, and the Numerical keys dialog for entering
numerals.
Error
"Printer Error" occured.
Quit
Dialogs appear at the top of the screen displayed at that time. Buttons at
the bottom at the screen cannot be pressed then.
<Error dialog>
Store Setting data?
OK
Cancel
<Confirmation dialog>
Display column
ID
The Main screen sample ID, setting values, and other items which can be
changed by the operator appear as white boxes with shadows.
These boxes are referred to as display columns in the Instructions for
Use.
Press on a display column to input information.
Selections
Type1
Type1
Type2
Type3
For settings which require the selection of one setting from multiple
options, the options are displayed when the display column is pressed.
Press the selection to input that value. If all of the options cannot be
displayed on the screen, the [more] button is displayed in the options
area. Press the [more] button to change the displayed options.
Type4
Revised March 2006
more
6-4
Operation
Numerical keys dialog
The numerical keys dialog appears for entering numerals. The buttons
displayed differ depending whether numerical keys or alphabetical keys
are chosen. But the operations are the same.
ALPH
Ent.
Enter numerals.
.
Enter symbols.
Changes the entered value to zero in the
case of an input of numerals (when the
[ALPH] button is not displayed).
Erases one character in the case of an
input of characters (when the [ALPH]
button is displayed).
Ent.
ALPH
ALPH
alph
ALPH
NUM
(Enter button):
Confirms the value which was input, and

closes the numerical key dialog.
Also, alphabetic characters may be entered.

Pressing the
ALPH
once changes the key dialog to
alph
or
NUM
Press this switching button to change the characters which are displayed
and which can be input.
Switching button display
Example of other button

display
ALPH
alph
abc
Remarks
8
(numerical input)
(Input 7.)
A, B, C
(alphabetic capital letter input)
(Input space.)
a, b, c
(alphabetic small letter input)
(Input space.)
Revised March 2006
NUM
ABC
Example of characters
which can be input
6-5
Operation
Press the
ABC
button once to input A.
Press the
ABC
button twice to input B.
Press the
ABC
Pressing the
button three times to input C.

button four times returns to A.
ABC
While entering alphabetic characters, the characters are not confirmed.

To confirm the character, press another button or the [Ent.] (Enter)
button.
[Example]
Entering A BCD e
Button operation
Input status
Explanation
Changes to alphabetical capital letter
mode.
ALPH
Inputs A (not confirmed).
Confirms A.
AB
Inputs B (not confirmed).
AB
Confirms B.
A BC
Inputs C (not confirmed).
DEF
A BCD
Confirms C. Inputs D.
alph
A BCD
Confirms D. Changes to alphabetic

small letter mode.
A BCD e
Inputs e.
A BCD e
Confirms e.
ABC
Ent.
ABC
Ent.
ABC
def
Ent.
Confirms complete entry.

Closes numerical keypad.
Revised March 2006
Ent.
6-6
Operation
6.2 Menu tree

Manual Analysis
Manual Analysis
Auto Rinse
Main
QC
Clog Removal
Drain TD Chamber
Result
Clean Transducer
Shutdown
Clean W. Chamber
Status Display
Menu
Calibration LCD
Str.Data
Print Error Log
Chg.Reag
Setting Seq.
Maint.
Drain Reagent
Auto Calib.
Calib.
Manual Calib.
Print Cal. His.
Settings
System
Date/Time
Species
Quality Control
User Information
Host Output
Built-in Printer
Revised March 2006
Network
Password
Print set value
6-7
Operation
6.3 Signal tones

This instrument indicates different situations by three distinct signal
tones.
1. Key tone (Short beep)
Beeps for about 0.1 seconds when pressing a button which is active
input.
2. Input errors (Short beep)
Beeps for about 1 second when pressing a button which is not active
input.
3. Analysis error
Continuously beeps if the instrument detects an error, and stops the
alarm sound when the [OK] button of the error dialog displayed on
the LCD screen is pressed. Settings can be used to change the tone.
See Chapter 12. Instrument Setup.
Important!
When an alarm is sounded, the alarm sound can be
stopped if the [OK] button of the error dialog
displayed on the LCD screen is pressed. Press the
button to display the help menu. All other
buttons are not functional during an alarm.
6.4 Timer functions

This instrument includes 3 types of timers.
1. LCD backlight dimmer timer
Purpose:
Reduces power consumption and extends backlight lifetime.
Operation:
Dims the backlight.
Timing:
Activates when instrument is left for approx. 10 minutes with no

screen operations.
Cancel:
Touch any part of the LCD screen, or open and close the sample
position once.
Purpose:
Prevents drying of the hydraulic line.
Operation:
After the specified amount of time has passed, automatically cleans

the hydraulic line before starting analysis (during the time after the
[RUN] button was pressed and before analysis begins).
Timing:
Activated when the instrument has not been operated for 2 hours.
Cancel:
Analysis begins after cleaning is completed.
3. Shutdown warning timer
6-8
Purpose:
Suggests that the operator initiate the shutdown sequence.
Operation:
Displays the shutdown warning message.
Timing:
Activates after 24 hours.
Cancel:
Perform shutdown, then turn the main power switch OFF-ON.
Revised March 2006
2. Automatic rinse timer
Sample Analysis
7. Sample Analysis
7.1 Introduction
This chapter explains the entire operating procedure from startup to
shutdown of the instrument, as well as the operating procedure
7.2 Flowchart of analysis procedure

The flowchart below shows the operating procedure and reference pages.
Page 7-2
Switching ON
Page 7-3
Quality control
Page 7-4
Analysis
Page 7-5
Display of analysis results
Page 7-13
Print and output of analysis results
Page 7-13
End of operation (Shutdown)
Page 7-14
Revised March 2006
Checks prior to operation
7-1
Sample Analysis
7.3 Checks prior to operation

Perform the following checks before turning the main power switch of
the instrument ON.
Power cable
Ensure that the power cable is properly connected.

The power cable must be plugged into an outlet.
Waste bottle
Biological risks
Always wear protective garments and gloves
when handling the waste fluid. After work is
completed, wash hands with disinfectant.
When handling (disposing) the waste fluid,
dispose it appropriately in accordance to local
laws and regulations, which considers for the
disposal of medical waste and infectious waste.
1. Check if the waste bottle is not full and has enough room for a day's
work. If the waste bottle is nearly full, dispose the waste fluid.
2. Make sure the cap is fit.
3. Check that the tube is not bent.
Printer paper
1. Open the printer cover on the top of the instrument, and check that
the paper supply is adequate for the number of samples expected for
the day.
For the replacement procedure, see Section 13.15 Replace thermal
printer paper.
2. Check to see that the paper is properly aligned.
Revised March 2006
Important!
Use only printer paper recommended by Sysmex.
Low quality paper may shorten the life of the printer
head.
7-2
Sample Analysis
7.4 Switching ON
After completion of all checks, the main switch can be turned ON.
Set the main power switch to position "I" ON.

The LCD screen will be illuminated. The program version will be
briefly displayed.
After the self-check, auto rinse, and background check are
completed, the Main screen will appear. It will take 6 to 11 minutes
before the Main screen appears.
Self-check
Running
Top
Start up
< Scheduled Maintenance >

Set a detergent in the sample
position.
It will take approx. 13 minute(s).
Cycles after cleaning
1513
Last maintenance date 31/12/2000
Clean W. Chamber.
Execute Cancel
After the main power switch is turned ON, the instrument performs a
Self-check. This process takes approximately 2 minutes.
In order to ensure optimal instrument conditions for use at all times,
certain parts require periodic maintenance. This instrument includes
counters which record the number of times that each of these parts is
used. If the counter value is above 150 when the main power switch is
turned ON, or if 2 weeks have passed since the previous maintenance, a
screen will appear prompting the operator to perform periodic
maintenance, i.e. transducer cleaning. If the counter value exceeds 1500,
or if 3 months have passed since the previous maintenance, a screen will
appear prompting the operator to perform periodic maintenance, i.e.
waste chamber cleaning. Follow the instructions on the screen and
perform the required maintenance.
If an error is detected during the Self-check, an error message appears on
the LCD screen. Turn the instrument main power switch OFF.
Wait 15 seconds, then turn it ON again.
Background check
Running
Start up
< Auto Rinse >
Revised March 2006
Please wait.
Menu
After completion of the Self-check, three automatic rinse cycles are

performed. Approximately 1 minute is required for the automatic rinse
cycle, and approximately 2 to 7 minutes are required for the background
check. After the third rinse cycle, a background check is performed.
Should any of the values be out of the acceptable limit (see Section
15.1 Performance characteristics/Specifications), a maximum of two
extra Background checks will be performed. If afterwards a value is still
out of the acceptable limit, a continuous alarm sounds and the message
Blank error is displayed on the LCD screen.
Press the [OK] button on the error dialog. The alarm sound stops.
Press the
button and the action message will appear.
7-3
Sample Analysis
Running
Start up
< Background check >
Menu
Perform the appropriate action according to the action message

screen.
When the automatic rinse and the background check are completed
normally, the Main screen will appear.
Press the [Result] button and Background check result will appear.
Please wait.
Note:
"Blank Error" may occur, if the instrument is not operated for
several days.
7.5 Quality control

Important!
Always perform a Quality Control prior to operation as
described in Chapter 10. Quality Control.
7.6 Specimen requirements

Conditions of collection
Venepuncture specimens should be collected into EDTA anticoagulant
(EDTA-2K, EDTA-3K or EDTA-2Na) and processed within 4 hours of
collection. If specimens cannot be processed within 4 hours, they should
be refrigerated at 2 to 8C. Before processing refrigerated specimens
should be allowed to warm up to room temperature (minimum 15
minutes), then mixed, preferably by rotation, for at least 2 minutes.
Stability of whole blood specimens
Revised March 2006
When the specimen is left unrefrigerated for more than 4 hours certain
changes occur within blood cells, which may produce misleading results
of clinical significance. Red blood cells swell, the MCV increases. The
total WBC count may decrease and the reliability of the electronic
differential white blood cell count diminishes. The degree of change is
variable depending on the specimen and the temperature at which it is
stored. These changes are largely prevented by storage at 2 to 8C.
7-4
Sample Analysis
7.7 Analysis
Sample preparation
The instrument can be used to analyze whole blood to which an EDTA
anticoagulant has been added.
The blood sample should be collected by vein-puncture.
Aspirated sample volume: Approximately 15 L
Caution!
Blood in which clotting or platelet aggregation has
occurred cannot be analyzed.
Important!
Mix the sample promptly after the blood is drawn.
If mixing is insufficient, platelet aggregation may
occur.
Perform analysis of the sample promptly after it is

mixed. If the sample is to be stored, refrigerate it
at 4C.
Let refrigerated samples adapt to room

temperature for at least 30 minutes before
performing the analysis.
Use sample tubes that are 85 mm or less in height

and that are 11 to 15 mm in diameter.
Revised March 2006
Note:
All performance claims given in this manual were
generated using specimens in EDTA anticoagulant. Results
may be affected by the use of other anticoagulants.
Therefore, each laboratory should develop protocols for
handling specimens collected in their anticoagulants.
7-5
Sample Analysis
Setting the species
Ready
Menu
Main
ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
The species setting immediately after turning ON the power is species

assigned on the upper left icon. To set the species, follow the procedures
below.
When species blood assigned on the icons are to be analyzed:
1. Be sure the status display indicates "Ready".
2. Press the icon which is desired.
RUN
select
QC
Result
Shutdown
Ready
Menu
Main
ID
Species (1)Dog
When any other species blood are to be analyzed:

1. Be sure the status display indicates "Ready".
(5) OTHER1
S
s (6) OTHER2
p
(7) OTHER3
2. Press the "select" button.

3. Species selection button (OTHER1, etc) will be displayed.
Press the desired species button.
X ^ [ g
select
QC
Result
more
1/6
close
Shutdown
Caution!
The analysis data of the OTHER1 - OTHER13
mode is for research.
Important!
This setting is kept until the species is selected in the
next time or the power switch is turned OFF then ON
again. Always check the species selection before
performing analysis.
Revised May 2006
Note:
Regarding the species setting, see "Species specific
settings" in Section "12.2 Possible settings".
7-6
Sample Analysis
Entering a sample ID
Sample ID can be entered by the following two methods.
Entering from the numerical keys dialog.
Entering from the handheld bar code reader (option).
The sample ID can be set up to 15 characters (alphanumeric characters,

hyphens and space) in length.
With the setting using ISBT128 bar code, up to 13 characters can be
used.
For setting method of ISBT128 bar code, see System setup in Chapter
12.2 Possible settings.
For entering numerals, see "Numerical keys dialog" in Chapter
6.1 Screen display.
Note:
If no sample ID is entered, it will be incremented by 1 for
each new analysis.
[Example]
123 124
9999999999999991
12-312-4
12-999 12-000
A999000
If the last character of the sample ID is an

alphabetic character, it will not be counted up
(incremented) automatically.
Revised March 2006
Caution!
When setting a sample ID to 0, the analysis
result will not be stored as data, and it will neither
be printed automatically with the built-in thermal
printer, nor can it be transferred to the host
computer automatically. Also, sample ID 0 will
not be incremented automatically.
7-7
Sample Analysis
Input via numerical keys dialog
Ready
Menu
Main
ID
1. Press the [ID] display column on the Main screen.

The numerical keys dialog will appear.
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Menu
Main
ID
Species (1)Dog
Set a mixed
sample,
4 and
5
Press [RUN].
QC
Result
ALPH
6
3
C
:XRUN
:^ :[ :g
select
2. Enter the sample ID.

During the sample ID input, the farthest right character can be
deleted by pressing the [C] button.
3. Press the [Ent.] button.
Sample ID is defined, and the status turns to Ready for starting
analysis.
Ent.
Shutdown
Input from handheld bar code reader (option)

After pressing the [ID] column, the bar code can be read in the status of
displaying the numerical keys dialog. When the displayed sample ID is
not correct, read the bar code again.
Make sure that the displayed sample ID is correct, and press the [Ent.]
button.
When the manual input is selected, bar code cannot be read. In this case,
press the [Ent.] button once, then press the [ID] column again to read the
bar code.
7-8
Reading error may occur when a bar code reader

is used without a check digit. It is recommended to
use a check digit or to check the sample ID after
entry.
Do not use any other characters than alphanumerics, hyphens and space on the bar code
label.
Revised May 2006
Caution!
When using a bar code label, affix it properly so it
is flat and smooth without creases or flares.
Sample Analysis
Analyzing samples
Caution!
When a tube with a large diameter (approximately
15 mm diameter) is used, it may be difficult to set
it in the sample position. Do not force the sample
tube into the sample adapter or force the sample
adapter into the sample position. This may
interfere with opening the sample position.
1. Press the top of the sample position to open it.
Important!
Push the top of the sample position to open it
automatically. If you attempt to force it open, the
instrument may be damaged.
If there is an object such as a pencil under the
sample position, it cannot be opened properly.
Close the sample position once, and make sure
that there are no objects underneath the sample
position.
Biological risks
Wear protective garments and gloves when
performing sample analysis. After completion of work,
wash hands with disinfectant.
2. Place the correct adapter in the sample position.
Adapter
Caution!
Be sure to use the correct adapter for the sample
tube being used.
The sample tube adapters (15 mm diameter tube
adapter and S-MONOVETTE (SARSTEDT) 2.7
mL adapter) are optional parts in some areas.
Revised March 2006
Adapter name
Product code
Adapter color
Sample tube adapter

(for 15 mm diameter tube)
442-3032-1
Black
Sample tube adapter

(S-MONOVETTE (SARSTEDT) 2.7 mL)
442-3038-3
Red
7-9
Sample Analysis
3. Mix the sample thoroughly.
The sample can be analyzed from either a sample tube or a micro
tube.
Important!
Vigorously mixing the sample can damage the blood
cells and cause bubbles to form in the sample. If this
occurs, correct analysis results will not be obtained.
4. Remove the sample tubes cap. Then, set it in the adapter and close
the door.
a: Sample tubes
Set a sample tube in the adapter, and close the door.
Warning!
When a sample tube with a cap is used, manually
remove the cap before analysis. The pipette might be
permanently damaged, if the cap was not removed.
b: Micro tubes
If the micro tube has a cap, remove it.
Adapter
Be sure to remove the cap.

Revised March 2006
442-3045-0
Adapter for
Control blood
Caution!
When using micro tubes, be sure to place them in
the correct adapter.
7-10
Sample Analysis
micro tube
Adapters are optional parts:
Menu
Main
ID
Species (1)Dog
Adapter
shape and color
033-0861-0
Purple
Fits with micro tubes of which diameter is

approx. 8 mm and length 35 mm or more
033-0891-1
Green

approx. 11 mm
033-0871-7
Yellow

approx. 8.5 mm
442-3044-6
Cream

approx. 8 mm and length less than 35 mm
033-0881-4
Blue

approx. 11 mm with higher or thick bottom
442-3042-9
Gray

approx. 10 mm
Caution!
When using a micro tube attached with a cap, such as
Eppendorf tube, set the cap part close toward you to
avoid mechanical problems.
micro tube
with a cap
Ready
Product code
5. Make sure that Ready is displayed on the instrument status

indicator at the top of the display. Check the analysis mode, then
press the [RUN] button.
Set a mixed
sample, and
Press [RUN].
Revised May 2006
RUN
select
QC
Result
Shutdown
7-11
Sample Analysis
Ready
Menu
Main
ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
Note:
If the [RUN] button is pressed without changing the
sample ID, a warning dialog will appear on the screen.
Warning
RUN
Start analysis without changing

select
the Sample
ID.
Result OK
QC
Cancel
Shutdown
Not Ready
Menu
Main
ID
Species (1)Dog
Set a mixed
sample, and
press [RUN].
Warning!
If the [RUN] button is pressed while the sample
position door is open, a warning dialog appears. Set a
tube adapter and close the door before pressing the
[RUN] button.
Warning
X ^ [ g
Please set a tube adapter and

select position.
Close the sample
Q @
OK
fl
Running
Top
Measure
ID 123456789012345
(1)Dog
WBC
RBC
HGB
HCT
PLT
x103/uL
x106/uL
g/dL
The analysis starts, and the status display reads Aspirating.

When sample aspiration is completed, the status display Aspirating
changes to Running.
By the time Running is displayed, the sample position door can be
opened and the sample can be removed safely.
%
x103/uL
Asp. is finished.
Important!
Do not open the sample position while Aspirating is
displayed. If the sample position is opened while
displaying Aspirating, a correct analysis result will
not be obtained.
The analysis result of WBC and HGB are displayed on the LCD screen
about 90 seconds after starting the analysis. The analysis results for all
parameters are displayed about 125 seconds after the start.
Revised May 2006
When the status display indicates Ready, the next sample can be
analyzed. Repeat the process as described above.
7-12
Sample Analysis
7.8 Display of analysis results

Not Ready
Result
Top
ID 123456789012345 01/01 12:10

[S] [W] [R] [P]
(1)Dog
ERROR WBC WL+
8. 9 x103/uL
RBC
5. 08 x106/uL
HGB
12. 9 g/dL
HCT
40. 3 %
PLT
223 x103/uL
The results of the last analysis performed analysis are displayed on the
LCD screen.
The complete display consists of 5 screen pages.
Switch the pages by pressing the [] buttons.
For further information regarding the displays, see Section 8.1 Latest
sample.
Press the [Top] button to return to the Main screen.
1/5
Print
HC Out
Note:
If no buttons on the screen are pressed, the screen will
automatically return to the Main screen when the next
analysis can be performed.
Press the [Result] button on the Main screen to display the
analysis results.
7.9 Print and output of analysis results

The analysis results can be printed from the built-in thermal printer or
output to the host computer.
The above-mentioned output devices can be selected from the screen
menu.
[Print]:The built-in thermal printer starts to print analysis results.
[HC Out]:Outputs the data to the host computer. It is possible to output
to either the serial port (RS-232C) or LAN (Ethernet), depending on the
user setting.
Revised March 2006
Note:
User settings can also be made for automatic output.
(See Chapter 12. Instrument Setup.)
7-13
Sample Analysis
7.10 End of operation (Shutdown)

At the end of the days analysis, before the instrument is turned OFF, the
Shutdown sequence should be executed.
Executing a Shutdown:
To be carried out all analysis have been performed, or at least once
every 24 hours, if the instrument is used in continuous operation.
Caution!
If the instrument is used continuously without
performing the shutdown sequence, protein build-up
on the internal parts may prevent obtaining correct
analysis results, and may damage the instrument.
Important!
If the instrument is turned OFF without executing a
Shutdown, water droplets may come out of the pipette
or deposits may build-up on the rinse cup.
Note:
It takes approximately two minutes to complete the
Shutdown sequence.
Ready
Menu
Main
ID
The shutdown confirmation dialog screen will appear.

1
Species (1)Dog
1. Press the [Shutdown] button when Ready status appears.
Set a mixed
sample, and
Press [RUN].
In case the [Cancel] button is pressed, the Shutdown is canceled,

and the instrument returns to the Main screen.
RUN
select
QC
Result
Shutdown
Not Ready
Top
Shutdown
2. Press the [Execute] button.
< Shutdown >

Run Shutdown, press [Execute].
Execute Shutdown.
7-14
Revised May 2006
Execute Cancel
Sample Analysis
Running
Shutdown
Top
< Shutdown >
Please wait.
3. Check that the shutdown sequence was completed and the display of
the shutdown completion screen.
Turn OFF the main switch on the right side of the instrument.
< Shutdown >

Remove tube from sample position
When the [Restart] button is pressed, the Main screen will appear.
and turn OFF the power.
Revised March 2006
Restart
7-15
Revised March 2006
Sample Analysis
7-16
8. Display and Output of Analysis Results

After each analysis the results are displayed on the LCD screen. The
analysis result screen is composed of 5 LCD screen pages. The
histogram, volume distribution flag, abnormal data flag, analysis error
flag, and analysis values are displayed.
First analysis result screen
Second analysis result screen
This instrument can store analysis results and histograms for up to 20

samples.
Third analysis result screen
The analysis results can be printed with the built-in thermal printer or it
can be output to the host computer.
Fourth analysis result screen
Important!
The QC data is also counted in this number.
Fifth analysis result screen
8.1 Latest sample

After each analysis the results are displayed on the LCD screen. Press
[] or [] button to scroll through the screen pages. The following are
displayed in succession:
First analysis screen
Displays analysis results of WBC, RBC, HGB, HCT, and PLT
parameters in large letters.
Not Ready
Top
Result
Species
Analysis error flag
Volume distribution
flag
Numeric value
abnormal flag
Sample ID
ID 123456789012345 01/01 12:10

[S] [W] [R] [P]
(1)Dog
ERROR WBC WL+
8. 9 x103/uL
RBC
5. 08 x106/uL
12. 9 g/dL
HGB
HCT
PLT
40. 3
223
Date and Time

of analysis
Manual Analysis
flag
Analysis data
%
x103/uL
1/5
HC Out
Revised March 2006
Print
8-1

Second analysis screen
Displays analysis results of WBC, RBC, HGB, HCT, MCV, MCH,
MCHC and PLT parameters.
Not Ready
Top
Result
ID 123456789012345 01/01 12:10

[S] [W] [R] [P]
(1)Dog
Species
WL+
WBC
RBC
HGB
HCT
MCV
MCH
MCHC
PLT
Volume distribution
flag
Numeric value
abnormal flag
Sample ID
8.9
5.08
12.9
38.6
76.1
25.4
33.4
223
x103/uL
x106/uL
g/dL
%
fL
pg
g/dL
x103/uL
Date and Time

of analysis
Analysis data
2/5
Print
HC Out
Third analysis screen

Displays a WBC histogram and analysis results of WBC, LYM%,
OTHR%, EO%, LYM#, OTHR# and EO# parameters.
Not Ready
Top
Result
Species
Sample ID
Analysis Mode
ID 123456789012345 01/01 12:10

[S] [W] [R] [P]
(1)Dog
ERROR
WBC
WBC Histogram
LD
T1
T2
8.9
31.0
8.5
60.5
2.8
0.8
5.3
x103/uL
%
%
%
x103/uL
x103/uL
x103/uL
Analysis data
3/5
HC Out
Revised March 2006
Print
30 fL
120 fL
180 fL
WBC WL+
LYM%
OTHR%
EO%
LYM#
OTHR#
EO#
Date and Time

of analysis
8-2

Fourth analysis screen
Displays RBC histogram and analysis results of WBC, RBC, HGB,
HCT, MCV, MCH, MCHC, RDW-SD and RDW-CV parameters.
Not Ready
Top
Result
Species
Sample ID
Analysis Mode
ID 123456789012345 01/01 12:10

[S] [W] [R] [P]
(1)Dog
ERROR
RBC
RBC Histogram
LD
35 fL
UD 250 fL
WL+ 5.07 x106/uL

RBC
HGB
12.9 g/dL
HCT
38.6 %
MCV
76.1 fL
MCH
25.4 pg
MCHC
33.4 g/dL
RDW-SD
43.1 fL
RDW-CV
14.9 %
Date and Time

of analysis
Analysis data
4/5
Print
HC Out
Fifth analysis screen

Displays PLT histogram and analysis results of PLT, PDW, MPV and PLCR parameters.
Not Ready
Top
Result
Species
Sample ID
Analysis Mode
ID 123456789012345 01/01 12:10

[S] [W] [R] [P]
(1)Dog
ERROR
PLT
PDW
MPV
P-LCR
PLT
PLT Histogram
LD
UD
WL+
223
7.6
10.9
18.0
x103/uL
fL
fL
%
Date and Time

of analysis
Analysis data
4 fL
20 fL
5/5
HC Out
Revised March 2006
Print
8-3

Description of the analysis results screen
ID
Displays the sample ID number.
Species
Displays the species selected at the time of analysis.
Date and time of analysis
Displays the date and time when the analysis result was obtained.
Analysis data
Displays the analysis data for the following parameters on the analysis
results screens.
First analysis result
screen [1/5]
Analysis parameters:
WBC, RBC, HGB, HCT

and PLT
Second analysis result

screen [2/5]
WBC, RBC, HGB, HCT,

MCV,MCH, MCHC and
PLT
Third analysis result

screen [3/5]
WBC, LYM%, OTHR%,

EO%, LYM#, OTHR# and
EO#
Fourth analysis result

screen [4/5]
RBC, HGB, HCT, MCV,

MCH, MCHC, RDW-SD
and RDW-CV
Fifth analysis result

screen [5/5]
PLT, PDW, MPV and

P-LCR
Note:
When the analysis was performed in the OTHER1 OTHER13 mode, LYM%, OTHR%, EO%, LYM#,
OTHR# and EO# are displayed as W-SCR, W-MCR,
W-LCR, W-SCC, W-MCC and W-LCC respectively.
8-4
Revised March 2006
Caution!
The analysis data of the OTHER1 - OTHER13
mode is for research.

Not Ready
Top
Result
ID 123456789012345 01/01 12:10

[S] [W] [R] [P]
(1)Dog
ERROR WBC WL+
8. 9 x103/uL
RBC
5. 08 x106/uL
HGB
12. 9 g/dL
HCT
PLT
40. 3
223
%
x103/uL
1/5
Print
HC Out
Analysis data without a preceding sign are within the preset limits.
Preceding signs indicate an analysis result which is out of the prescribed
limits:
Sign
Exposition
Value is out of the linearity limit.
Result exceeds the upper reference limit.
Result exceeds the lower reference limit.
Result is unreliable.
Note:
The values for + and - reference limits can be set by the
customer. Check the Reference Limit section in Chapter
12. Instrument Setup.
If an analysis error has occurred and a value is not available, one of the
following is displayed:
Display
Exposition
+++.+
Value exceeds display range.
***.*
Value could not be calculated because of analysis error.

At this time, the analysis error flag, [ERROR] (inverse in
coloration display) appears.
---.-
Value could not be calculated due to data error.
Note:
When the analysis was performed in the Cat mode,
PDW , MPV and P-LCR are displayed as spaces.
When the analysis was performed in the Cattle or
Horse mode, EO% and EO# are displayed as spaces.
In three histograms (RBC, WBC, PLT), the volume distribution is shown

graphically.
Revised May 2006
Flags are indicated by one of the following:

Flag
(1) WL
(2) WU
(3) T1
(4) T2
(5) F1
(6) F2
(7) F3
Exposition
Error at the lower discriminator for WBC
Error at the upper discriminator for WBC
Trough discriminator T1 could not be determined.
Trough discriminator T2 could not be determined.
Small cells are inaccurate.
Middle cells are inaccurate.
Large cells are inaccurate.
8-5

Flag
(8) RL
(9) RU
(10) DW
(11) MP
(12) PL
(13) PU
Exposition
Error at the lower discriminator for RBC
Error at the upper discriminator for RBC
Distribution width (20%) can not be calculated.
There are multiple peaks.
Error at the lower discriminator for PLT
Error at the upper discriminator for PLT
For details about sample flagging, see Chapter 8.5 Histogram error
flags.
Manual analysis flag
Displays manually analysed parameters.
Flag
[S]
[W]
[R]
[P]
Exposition
Species are changed.
Discrimination positions of WBC are moved.
Discrimination positions of RBC are moved.
Discrimination positions of PLT are moved.
WBC histogram
Displays a histogram for WBC.
RBC histogram
Displays a histogram for RBC.
PLT histogram
Revised March 2006
Displays a histogram for PLT.
8-6
8.2 Stored data

This instrument can store the analysis results of up to 20 samples. Data is
preserved, even after the instrument is switched OFF, and can be called
anytime as long as it is not deleted.
Important!
If there are already 20 data stored in the memory and
a new analysis is performed, the oldest data is
deleted (first in, first out).
The number of data stored in memory is twenty (20)
including QC analysis results.
Executing the stored data processing program
Ready
Menu
Main
ID
1. Press the [Menu] button on the Main screen.

1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data
Calib.
Chg.Reag
Settings
2. Press the [Str. Data] button.

The Stored data screen [1/5] (First analysis result screen) will appear.
Maint.
[] button:Moves to the previous analysis result.

However, when the oldest analysis result is displayed, this button
operation becomes invalid.
[] button:Moves to the next analysis result.
However, when the latest analysis result is displayed, this button
operation becomes invalid.
Revised May 2006
[] button:Switches the pages.
8-7

Finishing the stored data processing program
Not Ready
Top
Man. Ana.
ID 123456789012345 01/01 12:10

[S] [W] [R] [P]
(1)Dog
ERROR WBC WL+
8. 9 x103/uL
RBC
5. 08 x106/uL
HGB
12. 9 g/dL
HCT
PLT
40. 3
223
Press the [Top] button on the stored data screen.

Returns to the Main screen. Sample ID and analysis mode on the Main
screen will return to the settings before executing the stored data
processing program.
%
x103/uL
1/5
Print
HC Out
8.3 Manual Analysis

This menu allows the following:
Re-selection of species and re-calculation of data.
Relocation of particle distribution discrimination position and

re-calculation of data.
Caution!
Manual Analysis can not be performed on the data
with sample number 0.
Re-selection of species
Not Ready
Top
Result
ID 123456789012345 01/01 12:10

(1)Dog
WBC
8. 9 x103/uL
RBC
5. 08 x106/uL
12. 9 g/dL
HGB
HCT
PLT
40. 3
223
1. Press the
button on the Analysis screen.
The manual analysis confirmation dialog will appear.
%
x103/uL
1/5
HC Out
Not Ready
Top
Result
ID 123456789012345 01/01 12:10

(1)Dog
WBC
8. 9 x103/uL
RBC
5. 08 x106/uL
12. 9 g/dL
HGB
HCT
PLT
40. 3
223
2. Press the [Species] button.

The manual analysis screen will appear.
%
x103/uL
Revised March 2006
Print
1/5
Species WBC
8-8
RBC
PLT
Cancel

Not Ready
Top
Man. Ana.
3. Press the
button or the
button to select species.
ID 123456789012345 01/01 12:10

[S]
(3)Cattle
WBC
RBC
HGB
HCT
MCV
MCH
MCHC
PLT
9.0
5.07
12.9
38.6
76.1
25.4
33.4
235
x103/uL
x106/uL
g/dL
%
fL
pg
g/dL
x103/uL
Note:
When species are changed, the species name is
reverse-displayed, and the manual analysis flag [S] is
displayed.
1/4
4. Press the
button.
The analysis data is re-calculated on the basis of new species.
Not Ready
Top
Man. Ana.
ERROR
PLT
PDW
MPV
P-LCR
LD
UD
235
7.8
11.0
18.5
The complete display consists of 4 screen pages.

Switch the pages by pressing the
button.
5. Check the re-calculated analysis data, and press the [Top] button.
The save confirmation dialog will appear.
ID 123456789012345 01/01 12:10

[S]
(3)Cattle
PLT
Note:
Analysis values changed by re-calculations
are reverse-displayed.
x103/uL
fL
fL
%
4 fL
20 fL
[Save]
: Updates the results, and returns to the Analysis screen.
[Quit]
: Not update the results, and returns to the Analysis

screen.
[Cancel]
: Returns to the manual analysis screen.
3/3
Save results?
Save
Quit
Cancel
Caution!
When the [Save] button is pressed, the analysis data
is overwritten.
Relocation of discrimination position

Not Ready
Top
Revised October 2006
Result
ID 123456789012345 01/01 12:10

(1)Dog
WBC
8. 9 x103/uL
RBC
5. 08 x106/uL
12. 9 g/dL
HGB
HCT
PLT
40. 3
223
1. Press the
button on the Analysis screen.
The manual analysis confirmation dialog will appear.

2. Select a desired particle distribution.
The manual analysis screen will appear.
%
x103/uL
1/5
Species WBC
RBC
PLT
Cancel
8-9

Not Ready
Top
Man. Ana
ID 123456789012345 01/01 12:10

[W]
(1)Dog
WBC
LD
T1
T2
30 fL
126 fL
180 fL
WBC
LYM%
OTHR%
EO%
LYM#
OTHR#
EO#
8.9
33131.2
8.5
60.5
2.9
0.7
5.3
x103/uL
%
%
%
x103/uL
x103/uL
x103/uL
3. Press the
button to select the discriminator.
4. Press the
button or the
discrimination position.
button to move the
1/3
Note:
When the discrimination positions are changed, these are
reverse-displayed, and the manual analysis flags are
displayed.
[W]
Move the discri. positions of WBC
[R]
Move the discri. positions of RBC
[P]
Move the discri. positions of PLT
The range within which each discrimination position can move is

shown below.
WBC
Discri. to Move
Lower Limit
Upper Limit
LD
6 fL (0ch)
T1
T1
LD
T2
T2
T1
UD (49ch)
WBC particle distribution covers 50 channels of 0 to 49 (6 fL per

channel), and the channel changes by one at a time.
The relation between WBC particle distribution channel and fL
display is as follows:
Discri. position (fL) = (Channel No. + 1) x 6
Discri. to Move
Lower Limit
Upper Limit
LD
5 fL (0ch)
UD
UD
LD
250fL(49ch)
RBC particle distribution covers 50 channels of 0 to 49 (5 fL per

The relation between RBC particle distribution channel and fL
8-10
Revised March 2006
RBC

PLT
Discri. to Move
Lower Limit
Upper Limit
LD
1 fL (0ch)
UD
UD
LD
40fL(39ch)
PLT particle distribution covers 40 channels of 0 to 39 (1 fL per

The relation between PLT particle distribution channel and fL
5. After Discri. movement, press the
button.
The analysis data is re-calculated on the basis of new Discrimination

position.
Note:
Analysis values changed by re-calculations are
reverse-displayed.
Not Ready
Top
Man. Ana.
ID 123456789012345 01/01 12:10

[W]
[P]
(1)Dog
ERROR
PLT
PDW
MPV
P-LCR
PLT
LD
UD
223
7.7
11.1
18.3
x103/uL
fL
fL
%
4 fL
20 fL
6. Check the re-calculated analysis data, and press the [Top] button.
The save confirmation dialog will appear.
[Save]
:Updates the results, and returns to the Analysis screen.
[Quit]
:Not update the results, and returns to the Analysis

screen.
[Cancel]
:Returns to the manual analysis screen.
3/3
Save results?
Save
Quit
Cancel
Revised October 2006
Caution!
When the [Save] button is pressed, the analysis data
is overwritten.
8-11
Not Ready
Top
Man. Ana.
ID 123456789012345 01/01 12:10

[W]
[P]
(5)OTHER1-RESEACHERROR
PLT
PDW
MPV
P-LCR
PLT
LD
UD
223
7.7
11.1
18.3
x103/uL
fL
fL
%
4 fL
20 fL
3/3
Save this Disc. pos.?
Not Ready
OK
Cancel
Note:
When the analysis data of the OTHER1 - OTHER13
mode is saved, the discrimination position save
confirmation dialog will appear.
[OK] : Store new discrimination positions, and returns to
the main screen.
From the next measurement, particle distributions of the
other mode are discriminated with the new positions.
[Cancel] : Not store new discrimination positions, and
returns to the main screen.
Top
Disc. pos.
OTHER1
RBC LD
10 fL
RBC UD
250 fL
PLT LD
4 fL
PLT UD
20 fL
Note:
Discrimination positions for the other mode can be set in
the Settings - Species menu.
Save
8.4 Output
The analysis data can be output on the built-in thermal printer or the host
computer.
[Print]:The built-in thermal printer starts to print analysis results and
setting values, etc.
Important!
The [HC Out] button is displayed only when the host
computer is connected. See Chapter 12.2 Possible
settings.
For host computer output, install software compatible
with pocH-100iV Diff onto the computer to enable
data transfer.
8-12
Revised March 2006
[HC Out]:Outputs the data to the host computer. It is possible to output

to either the serial port (RS-232C) or LAN (Ethernet), depending on the
user setting.
8.5 Histogram error flags

Various information can be obtained from the histograms. The pocH100iV Diff extracts the characteristics of the histogram and displays
them as histogram flags. When the histogram flags are displayed,
perform analysis again. If afterwards the flags are still displayed, the
sample is considered to correspond to one of the following.
Revised March 2006
Flag
Probable sample cause
Correction
WL
Incomplete lysing of red blood cells, presence of

nucleated red blood cells, increase of large platelets,
platelet aggregation or agglutination, precipitation of
fibrin, presence of proteins or lipids.
Check smear.
Warm sample and repeat analysis.
If incomplete lysing is suspected, perform a 1:5
dilution of the sample (50 L of whole blood added
to 200 L of diluent) and re-analyze. Adjust the
results for the dilution factor.
RL
Presence of fragmented red blood cells, increase of

large platelets, platelet aggregation or agglutination,
presence of micro-red blood cells.
Check smear.
Manual count.
PL
Effects of cryoglobulins, fragmented red blood cells,

or cellular fragments of white blood cells.
Check smear.
Manual count.
WU
Incomplete lysing of red blood cells, presence of

immature white blood cells, white blood cell
aggregation, platelet satellite phenomenon, etc.
Check smear.
Dilute sample and repeat analysis.
RU
Effects of cold agglutinin, inclusion of white blood

cells.
Check smear.
PU
Increase of large platelets, inclusion of fragmented

red blood cells, effects of cryoglobulins, platelet
aggregation or agglutinative, presence of micro-red
blood cells.
Check smear.
Manual count.
Take an another blood sample.
DW
(RBC)
Significant anisocytosis, etc.
Check smear.
DW
(PLT)
Inclusion of fragmented red blood cells,

nonuniformity in size of platelets, effects of
cryoglobulins, etc.
Check smear.
If cyroglobulins are suspected, first warm the
sample and repeat analysis. If error message
persists, perform a plasma replacement (remove
plasma and replace with equal volume of diluent)
and repeat analysis.
MP
(RBC)
Effects of anemia treatment or blood transfusion

causing the presence of cells of multiple sizes.
Check smear.
MP(PLT)
Platelet aggregation, low platelet count.
Check smear.
8-13

Flag
Probable sample cause
Correction
T1
Presence of immature white blood cells, incomplete

lysing of red blood cells, etc., causing the first two
WBC populations in the WBC-Histogram not to be
separated.
Check smear.
T2

separated.
Check smear.
F1, F2, F3

separated.
Check smear.
Revised March 2006
Note:
Since cat platelets are known to be difficult to discriminate
from the red blood cells, it is a common problem in the cell
counters and a PU flag is often reported.
8-14
Output
9. Output
Analysis results, quality control and calibration data can be output to
the built-in thermal printer or transmitted to the host computer.
The above-mentioned devices can be selected from the screen menu.
[Print]:The built-in thermal printer starts to print analysis results and
set values, etc.
[HC Out]:Output the data to the host computer. It is possible to
output to either the serial port (RS-232C) or LAN (Ethernet),
depending on the user setting.
Device activation/deactivation
Ready
Menu
Main
1. Check to see that the device is properly connected and switched on.
ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu

Str.Data
Chg.Reag
Calib.
Settings
Maint.
Not Ready
Top
Setting
Note:
If a password has been set-up, the password input screen
will appear. Enter the password.
Revised May 2006
Enter Password.
9-1
Output
Not Ready
Top
Settings
System
Date/Time
Species
Quality Control
User Information
The first HC output setting screen will appear.

Host Output
Built-in Printer
Network
Password Setting
Print Settings
Not Ready
Top
HC Out.
Connect
Disable
Auto Out.
Enable
Format
pocH
T. Rate
9600bps
4. Press the [Host Output] button.
5. Press the Connect column, and select either Disable, Serial or

LAN.
6. Press the [Save] button to finish the setting change.
Data Len. 8bits
Save
Not Ready
Top
HC Out.
Connect
Disable
Auto Out.
Enable
Format
pocH
T. Rate
9600bps
Press the [OK] button to update the changed settings, and to

return to the main screen.
Press the [Cancel] button to discard all changes made, and the
dialog will be closed.
7. Press the [Top] button in order not to update the changed settings
and to return to a main screen.
Data Len. 8bits

OK
Quit
Cancel
Revised March 2006
Store Setting data?
The store setting data confirmation message will be displayed.
9-2
Quality Control
10. Quality Control

The reliability of this instrument and reagents is ensured by quality
controls. With the use of quality controls the stability of the measured
values is monitored over an extended period of time, and problems can
be detected early or prevented.
A quality control should be performed:
Before analyzing samples
After replacement of the reagent
After maintenance
If there is any doubt about the accuracy of the analysis values
As required by regulation(s)
10.1 Control material

The control materials, EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L
and EIGHTCHECK-3WP-H are used. These are equivalent to Low,
Normal and High level.
Important!
Do not use any other control material than
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L
and EIGHTCHECK-3WP-H. This control blood is
specially developed for the analyzer's measuring
technology.
When opening a new lot, the previously entered
assay values or lot numbers must be erased.
Product name
EIGHTCHECK 3WP-N
(ECN-110B) 1.5 mL 12 vials

(ECN-120B) 4.6 mL 12 vials
EIGHTCHECK 3WP-L
(ECL-110B) 1.5 mL 12 vials

(ECL-120B) 4.6 mL 12 vials
EIGHTCHECK 3WP-H
(ECH-110B) 1.5 mL 12 vials

(ECH-120B) 4.6 mL 12 vials
Revised March 2006
Intended purpose
Control blood material is used to test the precision and accuracy of
automated and semi-automatic hematology analyzers. Do not use
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK3WP-H for the calibration of the system!
10-1
Quality Control
Do not inject or ingest.
The human blood used for the production of EIGHTCHECK-3WP-N,
EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H has been tested
with licensed reagents (Food and Drugs Administration's Bureau of
Biologic, USA) and has been found non-reactive for Hepatitis B Surface
Antigen, Hepatitis C Virus (HCV) and HIV (HIV1+HIV2) Antibody.
However, none of the test methods known today can guarantee that
products based on human blood are not infectious. In keeping with good
laboratory practice, this product should be considered potentially capable
of transmitting infectious disease.
Composition
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK3WP-H contain stabilized human red blood cells, fixed human white
blood cells and a platelet component in a medium containing
preservatives.
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK3WP-H are to be stored upright at 2-8C before and after opening.
When handled in this manner, the unopened product is guaranteed stable
until the expiration date stated on the package.
After opening, the product is stable for 7 days if returned to the
refrigerator promptly after use.
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK3WP-H have been tested and found to provide stable parameter values
after at least 12 hours at room temperature (25C).
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK3WP-H are intended to be used with SYSMEX reagents and analyzers
only.
Note:
If other control materials are used the product performance
of Sysmex instruments cannot be guaranteed.
10-2
Revised March 2006
Refer to the instrument manual for further information.
Quality Control
Procedure
Preparing Control Blood
1. Remove a vial of control material from the refrigerator and
equilibrate to room temperature (18-30C) for 15 minutes before use.
2. Place the vial between the palms and roll it back and forth 10 times
(see illustration).
3. Turn the vial upside down and roll 10 more times.
4. Repeat steps 2 and 3 eight times or for a total of 2 minutes. Examine
the bottom of the vial and assure thoroughly mixing by confirming
that there is no pellet of cells adhering to the bottom of the vial
before performing the analysis. If there still is pellet of cells, repeat
step 3.
5. Using the appropriate adapter, analyze the control blood sample in
the same manner as whole blood, according to procedures listed in
the instruments Instructions for Use. Wipe threads of cap and vial
with clean lint-free tissue before replacing cap. Recap vial tightly.
6. Store at 2-8C in an upright position.
Note:
Always check with current control materials insert
instructions.
Manual procedures
Reference method can be applied to EIGHTCHECK-3WP-N,
EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H. Refer to a manual
for clinical laboratory procedures.
Revised March 2006
Methodology
Principle of the method
EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and EIGHTCHECK3WP-H are to be used as hematology control bloods for the quality
control of any Sysmex Fully Automated and Semi-automated
hematology analyzers. EIGHTCHECK-3WP-N is for the normal level,
EIGHTCHECK-3WP-L is for the low abnormal level and
EIGHTCHECK-3WP-H is for the high abnormal level.
The WBC histogram of EIGHTCHECK-3WP-N, EIGHTCHECK-3WPL and EIGHTCHECK-3WP-H indicate three part distributions when the
instruments particle distribution analysis is used.
The use of stabilized cell preparation for controlling hematology
instrumentation is an established procedure. When handled like a patient
sample measured in QC mode and assayed on a properly calibrated
(HBC, HCT) and functioning instrument, EIGHTCHECK-3WP-N,
EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H will provide
values within the expected range indicated on the assay sheet.
10-3
Quality Control
Performance characteristics and limitations of the method
A manual differential analysis of white blood cells cannot be
accomplished with EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L
and EIGHTCHECK-3WP-H.
The PRP (platelet rich plasma) method to assay the platelet count cannot
be accomplished with EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L
and EIGHTCHECK-3WP-H.
Traceability of control materials
The mean assay values (range) of EIGHTCHECK-3WP-N,
EIGHTCHECK-3WP-L and EIGHTCHECK-3WP-H are derived from
replicate analyses on whole blood calibrated instruments using the
reagents recommended by the equipment manufacturer.
The measuring results for EIGHTCHECK-3WP-N, EIGHTCHECK3WP-L and EIGHTCHECK-3WP-H should be within the relevant
expected range.
The expected ranges listed represent estimates of inter-laboratory
variation, which is usually accounted for by instrument calibration,
maintenance and operating technique.
For this reason, the assay values given are guidelines only used for the
control of the measuring system and are not absolute assays for
calibration.
Disposal
Expired EIGHTCHECK-3WP-N, EIGHTCHECK-3WP-L and
EIGHTCHECK-3WP-H should not be disposed in general waste.
Disposal procedures should meet the requirements of all applicable local
regulations.
References
Henry, J.B. Clinical Diagnostic and Management by Laboratory
Methods. Ed.17. W.B. Saunders.
Philadelphia, PA 1984.
Wintrobe, M.M. 'Clinical Hematology', 8th Edition, Lea and Febiger,

Philadelphia, 1981.
Department of Labor, Occupational Safety and Health

Administration. 29 CFR PART 1910. 1030: Occupational Exposure
to Bloodborne Pathogens: Final Rule.
Revised March 2006
10-4
Quality Control
10.2 Control methods

This instrument has following two quality control methods. Choose the
control method meeting your laboratory's internal regulations.
X control
Two consecutive analyses are performed (repeated determination) and
the mean of them is compared with the expected range.
Levey-Jennings control
Only one control blood analysis is performed (detection is separate
operation) and compared with the expected range.
10.3 Quality control process flow
Select quality control method.
Set control blood information.
Set when the instrument

is purchased and as
needed.
Set when a new lot of

the control blood is
used.
Perform quality control analysis.

Perform daily.
Revised March 2006
Check and record quality

control results.
10-5
Quality Control
10.4 Preparations
If the instrument has not been started up, turn the main power switch ON
and wait until the status display shows Ready.
Control method selection
Ready
Menu
Main
Follow the following procedures to switch to the control method.

ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
Result
Shutdown
Not Ready
Top
Menu
Str.Data
Chg.Reag
Calib.
Settings
Maint.
Not Ready
Top
Settings
Enter Password.
Not Ready
Note:
appear. Enter the password.
Top
Settings
System
Host Output
Date/Time
Built-in Printer
Species
Network
Quality Control
Password Setting
User Information
Print Settings
10-6
3. Press the [Quality Control] button.

A current control method will be displayed on the quality control
setting screen.
Revised May 2006
QC
Quality Control
Not Ready
Set QC
QC Method
Data Out.
Top
4. Select the control method.

X:
X control
L-J
Print
L-J: L-J control
5. Select a data output method.

This setting allows automatical output of results when quality control
analysis is completed.
Quit
Disable:No output.
Print: Output to the built-in thermal printer.
HC: Output to the host computer.
Print+HC: Output to both the built-in thermal printer and the host
computer.
Important!
When outputting to the host computer, the instrument
outputs to the serial port (RS-232C) or LAN (Ethernet),
depending on the connection settings for host computer.
(See Chapter 12. Instrument Setup.)
6. Press the [Quit] button.

The quit confirmation dialog appears.
Press the [OK] button to update quality control settings and

return to the Main screen.
Press the [Cancel] button to close the dialog.
In the quality control setting screen, pressing the [Top] button will
display the quit confirmation dialog.
Press the [Cancel] button to close the dialog to continue the

settings.
Revised March 2006
10-7
Quality Control
Settings for control blood information
Before using new control blood, input the control blood information into
the instrument.
Contents of these settings are the control blood lot ID, expiration date,
TARGET value and LIMIT values for each control parameter.
These can be entered by the following two methods.
Entering from numerical keys dialog
Entering from handheld bar code reader (option)
Quality control determines from two aspects of the instrument status

from two factors:
from accuracy - whether or not the analysis results are within the range
of TARGETLIMIT, and from the changes in analysis results over the
time. TARGETLIMIT values are referred to as the control limit values.
TARGET value and LIMIT values are different for each lot of the
control blood.
The instrument automatically determines accuracy from the TARGET
value and LIMIT values that were input into the instrument beforehand.
Changes in the analysis results over the time must be recorded by the
customer, by printing out results or an other means.
We recommend creating a chart as shown below, where a gradual
decrease can be observed.
+ LIMIT
TARGET
- LIMIT
Revised March 2006
This instrument stores the control blood information in 3 locations

(files). Therefore, it is possible to use 3 lots of control blood with
different IDs at a time.
10-8
Quality Control
Entering from numerical keys dialog
Ready
Menu
Main
ID
The file selection screen will appear.

This instrument memorizes three QC files.
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
1. Press the [QC] button at the Ready status.
RUN
select
QC
Result
Shutdown
Not Ready
Top
QC Analy
File
Lot ID
[1234567890]
[01/01/2002]
[1234567890]
[01/01/2002]
[1234567890]
[01/01/2002]
Ready
Top
QC Analy
[1]
File
Lot ID
[1234567890]
Expire
[01/01/2002]
2. Press the display column of the file to use.

The quality control analysis starting screen for the selected file will
appear.
Expire
First quality control analysis:

When no quality control has been executed yet, the parameter
column is a blank.
Before performing QC analysis, input information on the control
blood (Lot ID, Expiration date, TARGET value, and LIMIT
value).
Place control blood

in tube adapter and
place it in the sample
position. Press [Run].

The QC file [1]setting screen will appear.
When the display column for the parameter to be set is pressed, the
numerical keys will be displayed, and the setting value can be input.
Run
Print
Settings
(1st analysis)
Not Ready
Top
Set QC
File[1]
Lot ID
1234567890
Select Input
parameters.
Expire
01/01/2002
Quit
Revised May 2006
Clear
4. Press the [Lot ID] column.

The numeric value input dialog will appear.
5. Input the lot ID.
Up to 10 digits can be input for the lot ID.
6. Press the [Expire] column.
The date input dialog will appear.
10-9
Quality Control
7. Input the expiration date.
Up to 10 digits can be input for the expiration date.
Note:
The operator must ensure the control blood is not beyond
expiration. The analyzer does not judge the expiration
dates entered in the QC file.
8. Press the [] or [] button.
When the [] button is pressed, the display changes from the first
QC file setting screen to the second screen. After the sixth screen,
pressing the [] button returns to the first screen. When the []
button is pressed, the display changes in reverse order.
Note:
There are six QC file setting screens.
Not Ready
Top
Set QC
File1
WBC
TARGET
LIMIT
6.7
X103/uL
RBC
X106/uL
HGB
0.0
0.0
HCT
0.0
0.0
MCV
0.0
0.0
fL
10. Input the TARGET value and the LIMIT value for each parameter.
Important!
There are 21 control parameters, and since not all of
these can be displayed on one screen, switch the
LCD screen by using the [] or [] button.
Save
Not Ready
Top
Set QC
TARGET
LIMIT
6.7
0.5
X103/uL
11. When the setting is completed, press the [Save] button. The quality
control setting storing confirmation dialog will appear.
Press the [OK] button to store the setting value, and either the X
first analysis start screen or the L-J analysis starting screen will
appear.
RBC
4.38
0.18
HGB
13.6
0.5
HCT
36.3
2.0
MCV
82.9
4.1
fL
X10 /uL
g/dL
In the quality control setting storing confirmation dialog, pressing the

[Top] button will display the quit confirmation dialog.
Save


settings.
Revised March 2006
Clear
The numeric input dialog will appear.
g/dL
Clear
File1
WBC
9. Press each of the parameter display columns.
10-10
Quality Control
Ready
Top
QC Analy
File1
WBC
TARGET
LIMIT
67
X103/uL
RBC
438
18
X106/uL
HGB
13.6
0.5
g/dL
HCT
36.3
2.0
Note:
To clear all of the set information, press the [Clear] button.
The deletion confirmation of quality control setting
message will be displayed.
MCV
82.9
4.1
fL
Press the [OK] button to return the control data to

default and to close the dialog.
Press the [Cancel] button to cancel control data

deletion and return to the previous screen.
N
A
Change
settings?
Cancel
OK
Entering from handheld bar code reader (option)

Ready
Menu
Main
ID
1. Press the [QC] button at the Ready status.

The file selection screen will appear.
This instrument memorizes three QC files.
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
When the quality control has not been executed yet, the parameter
column is a blank.
RUN
select
QC
Result
Shutdown
Not Ready
Top
QC Analy
File
Lot ID
[1234567890]
[01/01/2002]
[1234567890]
[01/01/2002]
[1234567890]
[01/01/2002]
Ready
Top
QC Analy
First of all, input information on the control blood (Lot ID,

Expiration date, TARGET value, and LIMIT value).
2. Press the display column of the file number to be used.
The quality control analysis starting screen for the selected file will
appear.
Expire
[1]
File
Lot ID
[1234567890]
Expire
[01/01/2002]
First quality control

The QC file setting 1 screen will appear.
Place control blood

in tube adapter and
Run
Settings
(1st analysis)
Revised May 2006
Print
10-11
Quality Control
Not Ready
Top
Set QC
4. Read the Lot ID bar code in the Assay Sheet.

The Lot ID will be displayed if correctly read. When the displayed
Lot ID is not correct, read the bar code again.
File[1]
Lot ID
1234567890
5. Read the expiration date bar code in the same way.
Select Input
parameters.
Expire
01/01/2002
Clear
Quit
Not Ready
Top
Set QC
File1
WBC
TARGET
LIMIT
6.7
X103/uL
RBC
X106/uL
HGB
0.0
0.0
HCT
0.0
0.0
MCV
0.0
0.0
fL
Clear
6. Press the [] or [] button.

When the [] button is pressed, the display changes from the first
QC file setting screen to the second screen. When the screen at the
sixth screen, pressing the [] button returns to the first screen.
When the [] button is pressed, the display changes in the reverse
order.
g/dL
Save
Note:
The QC file setting screens are consisted of sixth screens.
7. Read the each bar code in the Assay Sheet in the same way as 4.
Revised March 2006
In each parameter, bar code has the information of TARGET value

and LIMIT value. Only the item that appears in the display can be
input. When all parameters are input in the screen, switch the screen
by using [] button to continue input.
10-12
Quality Control
Not Ready
Top
Set QC
File1
WBC
TARGET
LIMIT
6.7
0.5
X103/uL
8. When the setting is completed, press the [Save] button. The quality
control setting storing confirmation dialog will appear.
Press the [OK] button to store the setting value, and either the X
first analysis start screen or the L-J analysis-starting screen will
appear.
RBC
4.38
0.18
HGB
13.6
0.5
HCT
36.3
2.0
MCV
82.9
4.1
fL
X10 /uL
g/dL
Save
Clear
Ready
In the quality control setting storing confirmation dialog, pressing the

[Top] button will display the quit confirmation dialog.


settings.
Top
QC Analy
File1
WBC
TARGET
LIMIT
67
X103/uL
RBC
438
18
X106/uL
HGB
13.6
0.5
g/dL
HCT
36.3
2.0
Note:
To clear all of the set information, press the [Clear] button.
The deletion confirmation of quality control setting message
will be displayed.
MCV
82.9
4.1
fL
Press the [OK] button to return the control data to default

and to close the dialog.
Press the [Cancel] button to cancel control data deletion

and return to the previous screen.
N
A
Change
settings?
Cancel
OK
10.5 Performing a quality control

Biological risks
Wear protective garments and gloves when you
analyze the control blood. After completion of work,
Ready
Menu
Main
ID
1. Press the [QC] button on the Main screen.

The analysis control file selection screen will appear.
Species (1)Dog
Set a mixed
sample, and
Press [RUN].
RUN
select
Result
Shutdown
Revised May 2006
QC
10-13
Quality Control
Not Ready
Top
QC Analy
File
Lot ID
The analysis starting screen will appear.

Expire
[1234567890]
[01/01/2002]
[1234567890]
[01/01/2002]
[1234567890]
[01/01/2002]
Ready
Important!
Before performing analysis, it is necessary to select a
control method and enter the control blood
information.
Top
QC Analy
[1]
File
Lot ID
[1234567890]
Expire
[01/0 1 / 2 0 0 2 ]
2. Press the display column of the analyzing file.
3. Ensure that the status display indicates Ready.
Place control blood

in tube adapter and
Run
Print
Settings
(1st analysis)
Important!
Press the top of the sample position to open it
automatically. If you attempt to force it open it, the
position. If there is an object such as a pencil
under the sample position, it cannot be opened
sufficiently.
5. Set the control blood adapter in the sample position.
Revised March 2006
Caution!
Be sure to use the supplied sample adapter when
analyzing control blood. Failure to do so may result in
permanent damage to the instrument.
10-14
Quality Control
6. Mix the control blood in accordance with the package insert of the
control blood. (See Section 10.1 Control material).
Important!
The control blood (EIGHTCHECK-3WP) remains
usable for the specified period of days in the package
insert. If it is used after this point, abnormal count
values may result.
7. Open the control blood cap carefully to avoid splashing of blood. Set
the control blood into the control blood adapter, and close the sample
position.
Revised March 2006
Control Blood
10-15
Quality Control
X Control
Ready
Top
QC Analy
[1]
File
Lot ID
[1234567890]
Expire
[01/0 1 / 2 0 0 2 ]
Press the [Run] button.
Place control blood

in tube adapter and
Important!
The analysis starts, the status display reads
Aspirating.
When sample aspiration is completed, status display
Aspirating changes to Running.
When Running is displayed, the sample position
door can be opened and the control blood can be
removed safely.
not be obtained.
Run
Print
Settings
Running
QC Analy
(1st analysis)
Top
The Analyzing screen will appear.
File [1] Lot [1234567890] Exp. [01/01/2002]

X1
X2
X
Judgment
WBC
RBC
HGB
HCT
MCV
MCH
MCHC
PLT
Not Ready
QC Analy
Top
File[1] Lot[1234567890] Exp.[01/01/2002]

X1
X2
X
Judgment
WBC
7.6
RBC
4.56
HGB
12.7
HCT
56.3
MCV
46.5
MCH
34.2
MCHC
85.4
PLT
278
Back
Next
Use the [] or [] button to scroll the screen pages.
To have the results from the first analysis printed out on the built-in
thermal printer, press the [Print] button.
Important!
The print format cannot be changed.
Revised March 2006
Print
After completion of the first analysis, these analysis results will be

displayed in the X1 column on the analysis result screen.
10-16
Quality Control
Ready
Top
QC Analy
[1]
File
Lot ID
[1234567890]
Expire
[01/01/2002]
Press the [Next] button to adopt data from the first analysis and
perform the second analysis. If the analysis results are not to be
adopted, you can perform a new analysis by pressing the [Back]
button.
In either case, remove the control blood, close the cap, and mix well.
Then, remove the cap again, set the control blood to the sample
position, and press the [Run] button.
When the [Top] button is pressed the quality control analysis

discontinuance confirmation message will be displayed.
Press the [OK] button to discard all analysis results and return to the
Main screen.
Press the [Cancel] button to return to the previous screen.
Place control blood

in tube adapter and
Run
Print
Settings
(2nd analysis)
Not Ready
Top
QC Analy
[1]
File
Lot ID
[1234567890]
Expire
[01/01/2002]
Place control blood

in tube adapter and
Run
Settings
QuitPrint
QC Analysis?
Not Ready
QC Analy
(2nd analysis)
OK
Cancel
Top
File[1] Lot[1234567890] Exp.[01/01/2002]

X1
X2
X Judgment
WBC
7.6
7.5
7.6
RBC
4.66
4.56
4.76
+
HGB
12.7
12.5
12.6
HCT
56.5
56.3
56.7
46.4
46.5
46.3
MCV
34.2
34.0
34.1
MCH
86.0
85.7
85.4
MCHC
280
279
278
PLT
Print
Back
When the second analysis is completed, the analysis results from this
analysis will be displayed in the X2 column on the analysis result
screen. The mean values of the analysis results from the first and the
second analyses will be displayed in the X column.
Press the [Print] button to print the results from the second analysis.
When the [Top] button is pressed, the quality control analysis

discontinuance confirmation message will be displayed.
Press the [OK] button to discard the analysis results and return to the
Main screen.
Press the [Cancel] button to return to the previous screen.
Press the [Quit] button to adopt the results of the two analyses, and
output the mean of the two analyses to the built-in thermal printer or
host computer, depending on the quality control data output settings.
If the results of the second analysis are not to be adopted, press the
[Back] button to perform a new second analysis.
Quit
When performing re-analysis, remove the control blood, close the

cap, and mix well. Then, remove the cap again, set the control blood
to the sample position, and press the [Run] button.
Revised March 2006
Note:
The analysis results are saved to the stored data.
10-17
Quality Control
Example:
Sample IDs when quality control analysis was performed with file
No. 3 will be shown as the following:
Ready
Menu
Main
ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
Error
ResultOK
QC03-1
X2 analysis results:
QC03-2
Mean of X1 and X2:
QC03 (The mean does not contain

histogram data.)
From the stored data screen, the result data can be printed out or
transferred to the host computer.
For parameters with a mean of the two analyses that is beyond the
control limit, a + is displayed in the Judgment column, and a - is
displayed if the mean is below the lower limit. Then an alarm sounds and
the QC error message is displayed. Press the [OK] button to turn off the
alarm.
RUN
Select
"QC (X) Error" occurred.
QC
X1 analysis results:
Shutdown
Important!
For details about actions to resolve the QC (X) error,
see Chapter 14. Troubleshooting.
Levey-Jennings Control
Ready
Top
QC Analy
[1]
File
Lot ID
[1234567890]
Expire
[01/0 1 / 2 0 0 2 ]
Place control blood

in tube adapter and
Run
Print
Settings
Running
QC Analy
(2nd analysis)
Top
Press the [Run] button.

Important!
When analysis starts, the status display reads
Aspirating.
When sample aspiration is completed, the status
display Aspirating changes to Running.
door can be opened and the control blood can be
removed safely.
not be obtained.
The Analyzing screen will appear.
10-18
Revised May 2006
File[1] Lot[1234567890] Exp.[01/01/2002]

Data
Judgment
WBC
RBC
HGB
HCT
MCV
MCH
MCHC
PLT
Quality Control
Not Ready
QC Analy
Top
File[1] Lot[1234567890] Exp. [01/01/2002]

Data
Judgment
WBC
7.6
+
RBC
4.56
HGB
12.7
HCT
56.3
46.5
MCV
34.2
MCH
85.4
MCHC
278
PLT
Print
Back
The analysis values are compared to the target range.

To print results on the built-in thermal printer, press the [Print]

button.
Next
Not Ready
Top
QC Analy
[1]
File
Lot ID
[1234567890]
Expire
[01/01/2002]
After completion of a single analysis, the results are displayed on the

LCD screen.
Important!
The print format cannot be changed.
When the [Top] button is pressed before defining the quality control
data, the quality control analysis discontinuance confirmation
message will be displayed.
Press the [OK] button to discard all analysis results, and complete
the quality control program. Then returning to the main screen.
Press the [Cancel] button to return to the analysis starting screen.
Then to continue the quality control analysis.
Press the [Quit] button to accept the analysis results, and output the
results to the built-in thermal printer or host computer, depending on
the quality control data output settings. If the results of the analysis
are not to be accepted, press the [Back] button to perform a new
analysis.
When performing re-analysis, remove the control blood, close the
cap, and mix well. Then, remove the cap again, set the control blood
in the sample position, and press the [Run] button.
Place control blood

in tube adapter and
Run
Settings
QuitPrint
QC Analysis?
(2nd analysis)
OK
Cancel
Note:
The analysis results are saved to stored data.
Example:
When quality control analysis was performed with file No. 3, the
data will be stored as:
Analysis results:
QC03
From the stored data screen for output, the result data can be printed
out or transferred to the host computer.
For parameters values that are beyond the control limit, a + is
displayed in the Judgment column, and a - is displayed if the value
is below the lower limit. Then an alarm sounds and the QC error message
is displayed. Press the [OK] button to turn off the alarm.
Revised March 2006
Important!
For details about actions to resolve the QC (L-J) error,
see Chapter 14. Troubleshooting.
10-19
Quality Control
10.6 Recording QC data
Revised March 2006
Always record the results of quality control, and print a hard copy for
your records.
10-20
Calibration
11. Calibration
Calibration is performed to compensate for any reproducible
inaccuracies of the system. The HGB and/or HCT values are corrected
by a calibration value.
In automatic calibration, the reference values of 5 samples are entered.
The instrument determines the calibration value automatically by
analyzing those 5 samples.
In manual calibration, the calibration value must be calculated
according to a designated formula and entered.
The instrument needs to be calibrated:
before initial operation (Initial calibration is performed based on the

Sysmex Corp. standard device at the time of shipment from the
factory.);
when quality control shows deviations in the same direction which

are determined repeatedly;
when a major component of the instrument has been replaced.
Caution!
Calibration does not need to be performed at
specific intervals.
Follow your internal laboratory regulations for
performing calibration, as required.
Abnormal QC data due to instrument problems,
reagent degradation, or deterioration of control
blood cannot be eliminated by calibration.
Calibration is to compensate the analysis results
in a way, and all the analysis results will be
compensated to add a specific bias.
Note:
Upon calibration, run a QC using control bloods and verify
that the analysis results will not exceed the control limits.
11.1 Samples used for calibration
Revised March 2006
For calibration, use five or more samples of fresh normal human blood
meeting the following conditions:
Blood from healthy person who is not taking any medication
Blood added with an appropriate amount of anticoagulant
Per-sample whole blood volume to exceed 2 mL
HGB value to exceed 10.0 g/dL
HCT value to be within 35.5% and 55.5%
11-1
Calibration
11.2 Establishing reference values

Reference values for calibration of HGB and HCT values are determined
on another calibrated instrument.
Recommended measuring methods:
HGB: Determination of hemoglobin concentration (DIN 58931)
HCT: Determination of the concentration of blood corpuscles in blood
(DIN 58933)
Important!
Each sample should be analyzed at least three times.
Mark or number the samples and note of the values determined.
11.3 Automatic calibration

Select mode and parameter
Ready
Menu
Main
ID
1. Press the [Menu] button at the Ready status.

1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data
The calibration menu screen will appear.
Maint.
Settings
Revised May 2006
Calib.
Chg.Reag
2. Press the [Calib.] button.
11-2
Calibration
Not Ready
Top
Calib.
Note:
appear. Enter the password.
Enter Password.
Not Ready
Top
Calib.
3. Press the [Auto Calib.] button.

The automatic calibration target value setting screen will appear.
Auto Calib.
Manual Calib.
Print Cal. His.
Not Ready
Top
Auto Cal.
HGB(g/dL)
HCT(%)
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
Select Calibration
parameters.
Next
Entering target values

Not Ready
Top
Auto Cal.
HGB(g/dL)
HCT(%)
14.2
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
4 Calbration
5
6
Select
parameters.
1
Quit
1. Enter the reference values determined into the Target column.

When the button for the Target is pressed, the numerical keys dialog
will be displayed.
After entering a target value, press the [Ent.] button. The entered
value will be defined.
C
Ent.
Important!
Set 0 for the parameter that is not being calibrated.
Revised March 2006
Next
11-3
Calibration
Not Ready
Top
Auto Cal.
HGB(g/dL)
The automatic calibration target value confirmation message will be

displayed.
HCT(%)
14.2
38.4
13.8
35.5
14.5
35.5
14.1
36.3
13.6
37.2
2. After completion of entering target values, press the [Next] button.
Select Calibration
parameters.
Next
Not Ready
Top
Auto Cal.
HGB(g/dL)
1
14.2
HCT(%)
1
38.4
13.8
35.5
14.5
35.5
14.1
36.3
13.6
37.2
Select Calibration
parameters.
OK
Set target value?
Cancel
Next
Press the [OK] button to define target values, and display the
automatic calibration analysis starting screen.
Press the [Cancel] button to close the dialog, and the setting can
be continued.
* Press the [Top] button to display the setting cancel
confirmation dialog.
changing any setting, and the [Cancel] button to close the
dialog and then to continue the setting.
Performing analyses
Ready
Top
Auto Cal.
Data
HGB HCT
Current Cal.(%)
New Cal.(%)
Quit
Running
Target
Data
HGB HCT HGB HCT
14. 2 38. 4 14. 5 39. 2
13. 8 35. 5 14. 1 36. 1
14. 5 35. 5
14. 1 36. 3
13. 6 37. 2
Current Cal.(%)
New Cal.(%)
11-4
98. 5 100. 5
Run
Auto Cal.
1
2
3
4
5
Calib.(%)
HGB HCT
Top
Calib.(%)
HGB HCT
97. 9 98. 0
97. 9 98. 3
98. 7 103. 1
96. 6 101. 2
Set the sample in the sample position, and press the [Run] button to start
analysis.
Important!
When analysis starts, the status display reads
Aspirating.
When sample aspiration is completed, the status
display Aspirating changes to Running.
door can be opened and the sample can be removed
safely.
not be obtained.
Revised March 2006
1
2
3
4
5
Target
HGB HCT
14. 2 38. 4
13. 8 35. 5
14. 5 35. 5
14. 1 36. 3
13. 6 37. 2
When all target values have been entered, the instrument is ready for
analysis.
Calibration
The automatic calibration analyzing screen will appear.
Important!
It is important to analyze the sample belonging to the
reference value. The values of the sample to be
analyzed are indicated by the underline cursor.
When an analysis was performed, its values are listed in the Data
column. In the Calib. (%) column, the calculated calibration values are
shown. When the analysis of more than one sample is completed, the
calibration value is calculated automatically for each analysis, and it is
displayed at the bottom of the Calib. (%) column.
The mean calibration value for each analysis and the current calibration
value are used to calculate and display the new calibration value.
Note:
During analysis, if the mean calibration value for each
analysis exceeds 105% or is less than 95%, or if the new
calibration value exceeds 120% or is less than 80%, an
alarm sounds and the automatic calibration error
Press the [Cancel] button, and analyze again.
Perform analysis for 5 samples.
Updating calibration values
Not Ready
Top
Auto Cal.
Target
HGB HCT
1 14. 2 38. 4
2 13. 8 35. 5
3 14. 5 35. 5
4 14. 1 36. 3
5 13. 6 37. 2
Data
Calib.(%)
HGB HCT
HGB HCT
14. 5 39. 2 97. 9 98. 0
14. 1 36. 1 97. 9 98. 3
14. 3 35. 9 101. 4 98. 9
14. 2 36. 2 99. 3 100. 3
13. 7 37. 5 99. 3 99. 2
Current Cal.(%)
New Cal.(%)
OK
The calibration value change confirmation message will appear.
Press the [OK] button to update the calibration value, and to return to
the Main screen.
Press the [Cancel] button to return to the Main screen without

updating the calibration value.
Cancel
Revised March 2006
Apply New Cal.?
98. 7 103. 1
97. 9 102. 0
After completion of all analysis, press the [Quit] button. The automatic
calibration complete confirmation dialog will appear.
11-5
Calibration
11.4 Manual calibration

Important!
Use fresh normal human blood of 5 or more samples.
Calculating the calibration value
1. Establish the reference values as described in Chapter 11.2
2. Calculate the mean value.
3. Analyze the samples.
(See Chapter 7. Sample Analysis).
4. Calculate the mean value.
5. Calculate the calibration value using the following formula.
New calibration value =
Previous calibration value
Average of values gained by reference method

Average of values gained by this instrument
Example:
Average of HGB values gained by the reference method = 14.0 g/dL
Average of HGB values gained by this instrument = 14.2 g/dL
Previous calibration value of HGB = 98.7%
Calculation of the new calibration value:
98.7 (14 /14.2) = 97.30% (97.3% rounded off)
The calibration value of HGB needs to be set at 97.3%.
Updating calibration values
Ready
Menu
Main
ID

Species (1)Dog
Set a mixed
sample, and
Press [RUN].
RUN
select
Result
Shutdown
Revised May 2006
QC
11-6
Calibration
Not Ready
Top
Menu
Str.Data
The calibration menu screen will appear.
Chg.Reag
Calib.
Maint.
Settings
Not Ready
Top
Calib.
Note:
Enter Password.
Not Ready
Calib.
Top
3. Press the [Manual Calib.] button.

The manual calibration setting screen will appear.
Auto Calib.
Manual Calib.
Print Cal. His.
Not Ready
Manu. Cal.
Top
Calib.
HGB
98.7
HCT
103.1
Select Calibration
parameter.
Revised May 2006
Quit
11-7
Calibration
Not Ready
Top
Manu. Cal.
Calib.
HGB
97.9
HCT
103.1
4. Press the calibration value display column for the calibrated

parameter.
The numerical keys dialog will be displayed.
5. Enter the calibration value.
Enter numerical values, and press the [Ent.] button.

The entered value is defined.
Ent.
Quit
Not Ready
Top
Manu. Cal.
Calib.
HGB
97.9
HCT
102.0
Set New Cal.?
Note:
If nothing is input (space), the calibration value cannot be
defined.
6. After completion of entering the calibration values, press the [Quit]
button.
The manual calibration setting confirmation dialog will be displayed.
Select Calibration
parameter.
OK
Important!
A calibration error is displayed, if the mean calibration
value for each analysis exceeds 105% or is less than
95%, or if the new calibration value exceeds 120% or
is less than 80%.
The calibration value can be calculated using the
following formula.
Cancel
New calibration value

Calibration value =
100
Current calibration value

Press the [OK] button to update the calibration values, and to

updating the calibration values.
Revised May 2006
11-8
Calibration
11.5 Printing calibration history
Ready
Menu
Main
An overview of the five most recent calibration histories can be printed

out on the built-in thermal printer.
ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data
The calibrating menu screen will appear.
Chg.Reag
Calib.
Maint.
Settings
Not Ready
Calib.
Top
Note:
Enter Password.
Not Ready
Calib.
Top
3. Press the [Print Cal. His.] button.

The built-in thermal printer will print the calibration history.
Auto Calib.
Manual Calib.
Revised May 2006
Print Cal. His.
11-9
Revised March 2006
Calibration
11-10
Instrument Setup
12. Instrument Setup

By changing individual settings, you can adapt the instrument to your
needs or existing laboratory conditions.
Note:
Upon initial operation, some settings need to be updated.
Example: Current date and time
12.1 General information

Ready
Menu
Main

ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data
Calib.

Chg.Reag
Maint.
Settings
Not Ready
Top
Settings
Note:
Revised May 2006
Enter Password.
12-1
Instrument Setup
Not Ready
Top
Settings
The screen for the selected parameter will appear.

When the [] and [] buttons are displayed on the bottom of the
screen, the setting contents are divided into two or more pages. Pages
can be switched by pressing the [] or [] button.
Host Output
System
Built-in Printer
Date/Time
Species
Network
Quality Control
3. Select the button to be changed from the Settings menu.
Password Setting
User Information
Not Ready
Pirnt Output
Auto Pri.
Data
Format
Type1
Print Settings
Menu
4. Change the setting, and press the [Save] button.

The setting storing confirmation dialog screen will appear.
Press the [OK] button to update the changed settings and to

Press the [Cancel] button to close the dialog, and the setting can
be continued.
Press the [Top] button to display the setting cancel confirmation

dialog.

changing any setting, and the [Cancel] button to close the dialog
and then to continue.
Revised March 2006
Save
12-2
Instrument Setup
12.2 Possible settings

Note:
The underlined items are the initial settings made before
shipment from the factory.
System setup
Note:
Any language change takes effect only after the next startup.
Basic instrument settings:
Parameter
Setting
Units
Type1 / Type2 / Type3 / Type4 / Type5 / Type6

Type1: Japan
Type2: General Export
Type3: Canada SI
Type4: Dutch SI
Type5: Standard SI
Type6: Hong Kong SI
Setting changes made for this parameter become
valid the next time the main switch is turned ON.
For details, see Chapter 15. Technical
Information.
Language
Japanese / English
Setting changes made for this parameter become
valid the next time the main switch is turned ON.
Blood collection
tube
STANDARD / SARSTEDT
STANDARD:Standard sample tube
SARSTEDT:S-MONOVETTE (SARSTEDT)
sample tube
1/2/3
1: Quiet
2: Medium
3: Loud
Revised March 2006
Alarm sound
volume
12-3
Instrument Setup
Parameter
Setting
Timbre
Type1 / Type2 / Type3 / Type4 / Type5 / Type6

Type1: High continuous beep
Type2: Repeated high beeps
Type3: Repeated high two-tone beeps
Type4: Low continuous beep
Type5: Repeated low beeps
Type6: Repeated low two-tone beeps
ISBT128
Enable / Disable
When Enable is selected, the ISBT128 bar code
will be loaded as a sample ID number with up to
13 characters.
Important!
It is not possible to convert the stored data between
Dutch SI units and other units for the following 3
parameters: HGB, MCH, MCHC.
Do not use the stored sample data which was stored
before the setting change. In addition, renew settings for
reference limits, and for quality control TARGET and
LIMIT values.
Date/Time
The calendar and clock of the instrument can be set here.
12-4
Parameter
Setting
Format
Choices: yy/mm/dd / mm/dd/yy / dd/mm/yy
Year
Numeric input: 2000 - 2037
Month
Day
Hour
Minute
Revised March 2006
Note:
When time changes to summer or winter time, respectively,
the clock must be manually corrected accordingly.
Instrument Setup
Species specific settings
You can set the folloing parameters for each species.
Species
Parameter
Dog, Cat, Cattle, Horse
Reference limits, Correction values
OTHER1 - 13
Species name, Reference limits,

Correction values, Discrimination positions
Reference limits:
The upper and lower mark limits for the analysis results can be entered
here. If the analysis result exceeds the Upper Limit (UL), a + flag is
added next to that parameter. If the result is below the Lower Limit (LL),
a - flag is added. (See the table below for the initial settings.)
Revised May 2006
Parameter
LL (Lower Limit)
UL (Upper Limit)
WBC
RBC
HGB
0.0
0.0
HCT
0.0
0.0
MCV
0.0
0.0
MCH
0.0
0.0
MCHC
0.0
0.0
PLT
0.0
0.0
RDW-SD
0.0
0.0
RDW-CV
0.0
0.0
LYM% (W-SCR)
0.0
0.0
OTHR% (W-MCR)
0.0
0.0
EO% (W-LCR)
0.0
0.0
LYM# (W-SCC)
OTHR# (W-MCC)
EO# (W-LCC)
MPV
0.0
0.0
PDW
0.0
0.0
P-LCR
0.0
0.0
12-5
Instrument Setup
Note:
If LL or UL that satisfy the following conditions was entered
in the Patient Limit setting screen, a beep sounds and the
entering is canceled.
LL is higher than UL.
UL is lower than LL.
Correction values:
The correction values for HGB and HCT can be set here.
(See the table below for the initial settings.)
Parameter
Dog
Cat
Cattle
Horse
OTHER
1 - 13
HGB
100.2 %
100.3 %
97.6 %
87.2 %
100.0 %
HCT
92.4 %
78.3 %
89.7 %
92.5 %
100.0 %
Discrimination positions:
The discrimination positions for WBC, RBC, PLT can be set here.
Ready
Menu
Main
ID
Icon1
Icon2Set a mixed
sample, and
Press [RUN].
RUN
Icon3
Result
Shutdown
W-LD
54 fL
W-T1
132 fL
W-T2
144 fL
R-LD
10 fL
R-UD
250 fL
P-LD
1 fL
P-UD
20 fL
Parameter
Species
Icon1
Dog
Icon2
Cat
Icon3
Cattle
Icon4
Horse
Revised May 2006
QC
Other Icon4
OTHER1 - OTHER13
The species assigned on the icons in the main screen can be set here.
Species (1)Dog
Parameter
12-6
Instrument Setup
QC settings
Here the QC method and the data output method can be selected.
QC method /Data output setting

Parameter
Selection
QC Method
Choices: (X) / L-J
Data Output
Choices: Disable / Print / HC / Print + HC
User information settings

If several pocH-100iV Diff analyzers are connected to the host
computer, a unique naming can be set to identify each instrument so that
the unique naming is transmitted to the host computer along with the
analysis result.
Parameter
Selection
Unique naming
Alphanumeric input: Up to 15 digits of

alphanumeric character
Host settings
The method of data output to the host computer can be set here.
For details, see Chapter 15. Technical Information.
Revised August 2006
Parameter
Setting
Connect
Choices: Disable / Serial / LAN
Automatic Output
Choices: Enable / Disable
Format
Choices: pocH / KX-21N / ASTM / K-1000
Transfer Rate
Choices: 1200 / 2400 / 4800 / 9600 / 19200 bps
Data Length
Choices: 7 bits / 8 bits
Stop bit
Choices: 1 bit / 2 bits
Parity
Choices: Even / Odd / Disable
Protocol
Choices: Class A / Class B
Transfer Interval
Choices: 0 s / 2 s / 3 s / 5 s / 7 s / 10 s / 15 s
RTS/CTS
Choices: Enable / Disable
ID Pad.
Choices: 0 pad / SpacePad.
RDW
Choices: RDW-SD / RDW-CV
ASTM Rev.
Choices: 1381-95 / 1381-02
12-7
Instrument Setup
Printer settings
The method of printing out analysis results can be set here. The print
header can be set as desired, using information such as the laboratory
name or instrument name. See Section 15.3 Print formats.
For entering numerals, see "Numerical keys dialog" in Chapter
"6.1 Screen display".
Parameter
Setting
Automatic Print
Choices: All Data / Error Data / Disable
Format
Choices: Type1 / Type2 / Type3

Type 1: Prints 19 analysis results and
the histograms.
Type 2: Prints 19 analysis results.
Type 3: Prints only CBC8 analysis
results.
[First print header line]
Alphanumeric input:
Up to 16 digits of alphanumeric characters
[Second print header line]
Alphanumeric input:
[Third print header line]
Alphanumeric input:
Network settings
Address and other necessary settings can be made here for use of LAN
(Ethernet) port to communicate host computer. In addition, the MAC
address can be checked at this screen. For details, consult network
administrator at your laboratory.
Instrument side
Parameter
Setting
IP Address
Numeric input:
0 - 255 (common to all columns)
Default: 0.0.0.0
Netmask
Numeric input:
Default: 255.255.255.0
Default Gateway
Numeric input:
Default: 0.0.0.0
12-8
Revised March 2006
Instrument Setup
Host side
Parameter
Setting
IP Address
Numeric input:
Default: 0.0.0.0
Port Number

Default: 3000
12.3 Password setting

To prevent unauthorized change of the important settings of the
instrument, a password may be activated. The following functions are
password-protected:
Calibration functions
Setting functions
When any of the above functions is selected, you will be prompted with
the password entry screen.
Key in the password and confirm with the [Ent.] button.
Important!
In case the password has been forgotten, contact
your Sysmex service representative.
Password setting procedure
Ready
Menu
Main
ID

Species (1)Dog
Set a mixed
sample, and
Press [RUN].
RUN
select
Result
Shutdown
Revised May 2006
QC
12-9
Instrument Setup
Not Ready
Top
Menu
Str.Data

Chg.Reag
Maint.
Settings
Calib.
Not Ready
Top
Settings
Note:
If a password has already been set-up, the password input
screen will appear. Enter the password. (No password is set
when the instrument is shipped; the password input screen
will not appear then.)
Enter Password.
Not Ready
Top
Settings
System
Date/Time
Species
Quality Control
User Information
Not Ready
Password
3. Press the [Password Setting] button.

The password setting screen will appear.
Host Output
Built-in Printer
Network
Password Setting
Print Settings
Top
4. Press the New Password column.

The alphanumeric input dialog will appear. Set the password by
using maximum 10 digits of numerals (0-9) or hyphens (-). Then
press [Enter] button.
New Password
Retype Password
5. Press the Retype Password column.

Enter the new password to be set again, and then press the [Enter]
button.
6. Press the [Quit] button.
Revised March 2006
Quit
12-10
Instrument Setup
Ready
Not Not
Ready
Top
Top
Password
Password
New
Password
New
Password
Important!
If the New Password and the Retype Password are
not identical, the password setting error message will
appear.
Re-enter the password into both boxes.
**********
**********
Retype
Password
Retype
Password
**********
**********
OKOK
Passwords
match.
Passwords
do do
notnot
match.
Not Ready
Top
Password
New Password
When the New Password and the Retype Password are identical, the
store setting data confirmation message will be displayed.
Press the [OK] button to update the changed settings and to

Press the [Cancel] button to cancel the setting changes, and close
dialog.
Press the [Top] button on the setting screen. The setting stop
changing the settings. Press the [Cancel] button to close the
dialog and continue the setting.
**********
Retype Password
**********
OK
Cancel
OK
Revised March 2006
Store
Setting
Passwords
dodata?
not match.
12-11
Instrument Setup
12.4 Print set values

Ready
Menu
Main
To print a list of the current settings, proceed as follows:

ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data

Chg.Reag
Maint.
Settings
Calib.
Not Ready
Top
Settings
Note:
Enter Password.
Not Ready
Top
Settings
System
Date/Time
Species
Quality Control
The setting data will be printed out.

Host Output
Built-in Printer
Network
Password Setting
Print Settings
Revised May 2006
User Information
3. Press the [Print Settings] button.
12-12
Cleaning and Maintenance
13. Cleaning and Maintenance

To ensure proper functioning of the instrument, it is necessary to
periodically clean and service the instrument. Perform maintenance
according to the schedule below and record the results in the
Maintenance Checklist (See Chapter 19. Appendix).
Biological risks
To avoid the risk of infections, wear protective
garments and gloves for all cleaning or maintenance
work. After completion of work, wash hands with
disinfectant.
13.1 Maintenance schedule

Daily
Clean TD chambers and diluted sample lines (Shutdown)

(see Section 13.3)
Every 2 weeks (or every 150 samples)
Clean transducer (see Section 13.4)
Every 3 months (or every 1500 samples)
Clean waste chamber (see Section 13.5)
Perform Auto Rinse (see Section 13.6)
Dispose waste fluid (see Section 13.7)
Clean the sample tube adapter (see Section 13.8)
Remove a clog from transducer aperture (see Section 13.9)
Clean aperture of TD chamber (see Section 13.10)
Drain reagent (see Section 13.11)
Calibrate LCD (see Section 13.12)
Setting Sequence (see Section 13.13)
Replace reagent (see Section 13.14)
System fuse replacement (see Section 13.15)
Replace thermal printer paper (see Section 13.16)
List of consumables (see Section 13.17)
Check instrument status (see Section 13.2)
Print error log (see Section 14.4)
Revised March 2006
As-needed maintenance
13-1
13.2 Check instrument status

Ready
Menu
Main
ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
If you should contact the Sysmex Service Representative regarding any

instrument problem, you may be asked to check the operation counter,
program version, and other information.
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data
The maintenance menu screen will appear.
Chg.Reag
Calib.
2. Press the [Maint.] button.
Maint.
Settings
Not Ready
Top
Maint.
3. Press the [Status Display] button.

The status display screen will appear. The following information is
displayed on this LCD screen.
Auto Rinse
Status Display
Clog Removal
Calibration LCD
Vacuum: Current vacuum valve
Drain TD Chamber
Print Error Log
HGB Convert: HGB convert value
Clean Transducer
Setting Seq.
Sensor: ON/OFF status of each sensor
Clean W. Chamber
Drain Reagent
SV: ON/OFF status of each solenoid valve
Counter: Number of cycles since instrument production (not

resettable)
Waste_C: Number of cycles since last cleaning of waste chamber
Detector: Number of cycles since last cleaning of transducer
Version: Program version
Running
Maint.
Vacuum
HGB Convert
Sensor
SV
Counter
Waste C.
Detector
Version
Top
0. 0130 MPa
2012 (255)
1 2 3 4 5 6 7 8
1 2 3 4 5 6 7 8 9 0
1 2 3 4 5 6 7 8 9 0
1 2 3 4 5 6 7 8 9 0
1 2 3 4 5 6 7 8 9 0
1105
727 (17/05/2000)
727 (17/05/2000)
00-00 (BIOS: 00-00)
Note:
When the [Run] button is pressed, analysis operation starts,
and the instrument status can be checked during operation.
Revised May 2006
Run
13-2
13.3 Clean TD (transducer) chambers and diluted sample lines (Shutdown)

Deposits in the instrument hydraulic lines could cause problems.
Therefore, the transducer chambers and diluted sample lines must be
cleaned. This process is identical to the Shutdown sequence at the end of
operation.
Run the Shutdown when a days analysis is completed.
Perform this procedure at least once every 24 hours if the instrument is
on continuously.
Caution!
If the instrument is used continuously without
performing the Shutdown sequence, protein build-up
of the internal parts may cause incorrect analysis
results or it may damage the instrument.
Not Ready
Top
Shutdown
< Shutdown >

It takes approx. 2 minutes to complete the Shutdown

sequence.
Execute Cancel
Execute Shutdown.
Ready
Menu
Main
ID
Note:
If 24 hours have passed and Shutdown has not been
performed, a message appears prompting the operator
to perform Shutdown.
Species (1)Dog
Set a mixed
sample, and
Press [RUN].
1. Press the [Shutdown] button at the Ready status. The Shutdown

confirmation message will appear.
When the [Cancel] button is pressed, the Shutdown is canceled, and
returns to the Main screen.
RUN
select
QC
Result
Shutdown
Not Ready
Top
Shutdown
< Shutdown >

The Shutdown will be executed, and Shutdown processing screen
will appear.
Revised May 2006
Execute Shutdown.
Note:
Once the Shutdown sequence is started, it cannot be
canceled anymore.
Execute Cancel
13-3

Running
Top
Shutdown
< Shutdown >
Please wait.
3. Check that the Shutdown completion screen appears, and turn OFF
the main power switch on the right side of the instrument.
< Shutdown >
Remove tube from sample position
and turn OFF the power.
Restart
13.4 Clean transducer

Not Ready
Menu
Start Up

position.
153
Clean Transducer.
When the main power switch is turned ON, and if either the counter
value exceeds 150, or if 2 weeks have passed since the last maintenance,
a message will appear prompting the operator to perform periodic
maintenance (transducer cleaning).
Press the [Cancel] button to continue start up without executing the
cleaning transducer operation. Until the transducer is cleaned, the
message is displayed at start-up.
When this message is displayed, perform the transducer cleaning
according to the following procedures.
Execute Cancel
Not Ready
Top
Maint.
Status Display
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Setting Seq.
Clean W. Chamber
Drain Reagent
Note:
Even when the above-mentioned message is not displayed,
cleaning transducer can be executed by pressing the [Maint.]
button of the menu screen, then pressing the [Clean
Transducer] button of the maintenance screen
Revised March 2006
Auto Rinse
13-4

1. Prepare an empty sample tube. Use the supplied pipette to add at
least 3 mL of CELLCLEAN (for a 13 mm diameter sample tube).
Caution!
There is a potential that the detergent may corrode
the container. It is recommended to use a sample
tube made of glass or prepare the detergent
immediately before cleaning.
Important!
Push the top of the sample position to open it
automatically. If you attempt to force it, the
sample position, it cannot be opened sufficiently.
position.
3. Set the adapter in the sample position.
Revised March 2006
4. Set the prepared CELLCLEAN in place and close the sample

position.
13-5

Not Ready
Top
Start Up

Cleaning transducer will be executed, and the cleaning transducer
screen will appear.
Then, Auto Rinse and background checks will be performed.

position.
153
Clean Transducer.
Execute Cancel
Note:
When executing the transducer cleaning from the
maintenance menu screen, the transducer cleaning starting
screen will be displayed after the preprocessing sequence is
executed. Then press the [Execute] button.
Warning!
CELLCLEAN is a strong alkaline cleaning material. It
should not come in contact with skin or clothing. If it
happens, wash skin or clothing with plenty of water to
avoid injury or damage.
Running
Maint.
< Clean Transducer >
6. After completion of cleaning transducer, the Main screen will

appear.
7. Remove the tube from the analysis position.
Important!
When the cleaning is performed, the detector counter
(number of cycles since last cleaning of transducer) is
automatically reset.
Revised March 2006
Please wait.
Top
13-6
13.5 Clean waste chamber

Not Ready
Start up
Menu

position.
1513
Clean W. Chamber.
Execute Cancel
Not Ready
When the main switch is turned ON, and if either the counter value
exceeds 1500, or if 3 months have passed since the last maintenance, a
message will appear prompting the operator to perform periodic
maintenance (waste chamber cleaning).
Press the [Cancel] button to continue start up without executing

waste chamber cleaning operation. The message will appear each
time the unit is started until the cleaning is performed.
When this message is displayed, perform the waste chamber cleaning

as shown in the following procedures.
Top
Maint.
Auto Rinse
Status Display
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Setting Seq.
Clean W. Chamber
Drain Reagent
Note:
Even when the above-mentioned message is not displayed,
waste chamber cleaning can be executed by pressing the
[Maint.] button of the menu screen, then pressing the [Clean
W. Chamber] button of the maintenance screen.
1. Prepare an empty sample tube. Use the supplied pipette to add at

least 3 mL of CELLCLEAN (for a 13 mm diameter sample tube).
Caution!
There is a potential that the detergent may corrode
the container. It is recommended to use a sample
tube made of glass or prepare the detergent
immediately before cleaning.
Revised March 2006
Important!
Press the top of the sample position to open it
automatically. If you attempt to force it open, the
sample position, it cannot be opened sufficiently.
position.
13-7

3. Set the adapter in the sample position.
4. Set the prepared CELLCLEAN in place and close the sample

position.
Not Ready
Menu
Start up

position.
1513
Clean W. Chamber.
Execute Cancel
Running
Maint.
< Clean W. Chamber >
Cleaning waste chamber will be executed, and the cleaning waste

chamber screen will appear.
Then, Auto Rinse and background check will be performed.
Warning!
6. After completion of waste chamber cleaning, the Main screen will
appear.
7. Remove the tube from the sample position.
Important!
When the cleaning is performed, the waste chamber
counter (number of cycles since last cleaning of
waste chamber) is automatically reset.
Revised March 2006
Please wait.
Menu
13-8
13.6 Perform Auto Rinse

With the Auto Rinse function, all lines are cleaned and the waste is
drained.
Ready
Menu
Main
Execute Auto Rinse to perform a background check, or when the unit has
been left idle without performing Shutdown.
ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data
Chg.Reag
Calib.
Settings
Maint.
Not Ready
Top
Maint.
Status Display
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Setting Seq.
Clean W. Chamber
Drain Reagent
Not Ready
Top
Maint.
< Auto Rinse >

Run Auto Rinse, press [Execute].
Revised May 2006
3. Press the [Auto Rinse] button.

The Auto Rinse starting confirmation dialog will appear.
Auto Rinse
Run Auto Rinse.
Execute Cancel

The Auto Rinse and the Background check will be executed.
It takes approx 2 minutes to complete the Auto Rinse and approx 2 to
7 minutes to complete the Background check.
Should any of the value be out of the acceptable limit, maximum two
extra Background checks will be performed.
If afterwards a value is still out of the acceptable limit, a continuous
alarm is sounded and the message "Blank Error" is displayed on the
LCD screen.
Important!
See Chapter 14.3 to deal with Blank error.
13-9

Running
Maint.
Top
5. When the Auto Rinse and Background check are normally

completed, the Main screen will appear.
Press the [Result] button and Background check result will appear.
< Auto Rinse >
Please wait.
Running
Maint.
Top
< Background check >
Please wait.
13.7 Dispose waste fluid
Biological risks
Always wear protective garments and gloves when
handling the waste fluid. After work is completed,
When handling (disposing) the waste fluid, dispose it
appropriately in accordance to local laws and
regulations, which considers for the disposal of
medical waste and infectious waste.
Revised March 2006
If the "Drain Error" message appears and the waste bottle is full, follow
the procedure below to dispose the waste fluid.
13-10

1. Remove the cap from the full waste bottle. Pull the float switch
straight out to remove it.
Float switch
2. Dispose the waste fluid and clean the waste bottle out with water.
3. Insert the float switch into the empty waste bottle, and apply the cap.
Check that the tube is not bent.
Waste bottle
Not Ready
Menu
Help
Discard waste
Discard waste, and perform the error
recover sequence.
[Execute] Execute recovery sequence.
[Cancel] Back to the Prev. screen.
4. Press the [Execute] button on the error help screen. The waste
processing screen will appear, and the waste discharge operation will
be performed.
To return to the Main screen without performing the waste discharge
operation, press the [Cancel] button.
When the process is finished, the Main screen will appear.
ERROR CODE : xxxxx. xxxxx. xxxxx

Execute
Cancel
Caution!
Be sure to empty the waste bottle and insert the float
switch carefully, or the waste discharge operation
may fail.
Ensure that the waste bottle is secure and properly
connected before operating the instrument.
Waste fluid may discolor some materials.
If the waste fluid is spilt, wipe off with the damp cloth
immediately.
Clean spills according to your policies and

procedures.
Revised March 2006
13-11
13.8 Clean the sample tube adapter

If sample has been spilt into the sample tube adapter that is set in the
sample position, remove and clean the adapter.
Perform this task before the main switch is turned ON, or else turn the
main switch OFF before performing it.
Biological risks
cleaning the sample tube adapter. After work is
completed, wash hands with disinfectant.
1. Check that the main power switch is turned OFF.

Revised March 2006
3. Remove the sample tube adapter.
13-12

4. Clean the sample tube adapter either with distilled water or with a
solution of CELLCLEAN diluted by 10 times or more. If the
CELLCLEAN is used for cleaning, be sure to clean the adapter
afterwards with distilled water.
Warning!
happens, rinse skin or clothing with plenty of water to
5. Set the cleaned sample tube adapter in the sample position.
6. When the adapter has been set, close the sample position.
13.9 Remove a clog from transducer aperture

If an "Aperture Clog" error occurs, the clog must be removed.
Ready
Top
Help
Aperture Clog
Clog in the aperture is detected.
Execute Clog Removal Sequence
in case clog was not removed.
[Execute] Execute Clog Removal.
[Cancel] Back to the prev. screen
1. Press the [Execute] button on the "Aperture Clog" error help screen.
The clog removal screen will appear, and automatic clog removal
will be performed.
To return to the Main screen without performing automatic clog
removal, press the [Cancel] button.
When the process is finished, the Main screen will appear.
ERROR CODE : 331010.0.0

Execute
Cancel
Not Ready
Top
Maint.
Status Display
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Setting Seq.
Clean W. Chamber
Drain Reagent
Revised March 2006
Auto Rinse
Note:
Even when no error has occurred, automatic clog removal can
be performed by selecting [Maint.] from the Menu screen, and
then pressing the [Clog Removal] button on the maintenance
menu screen.
13-13
13.10 Clean aperture of TD (Transducer) chamber (Drain TD Chamber)

Biological risks
Wear protective garments and gloves when cleaning
aperture of transducer chamber (draining TD
chamber). After completion of work, wash hands with
disinfectant.
Ready
Menu
Main
If clogging of the aperture cannot be removed by cleaning the transducer

(cleaning sequence), the aperture must be cleaned manually with a
transducer brush.
ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu

Str.Data
Chg.Reag
Calib.
Settings
Maint.
Not Ready
Top
Maint.
3. Press the [Drain TD Chamber] button.

The drain TD chamber screen will appear.
Status Display
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Setting Seq.
Clean W. Chamber
Drain Reagent
Revised May 2006
Auto Rinse
13-14

Not Ready
Menu
Maint.

The sample fluid is drained from the TD chamber and a message
appears prompting the operator to turn the main power switch OFF.
Preparation for draining the TD chamber is completed.
< Drain TD Chamber >

Drain sample from TD Chamber,
and then, turn OFF the power.
When the [Cancel] button is pressed, the cleaning will be

discontinued, and the display returns to the Main screen.
Execute Cancel
Drain sample.
Running
Maint.
Top
Please wait.

Drain TD Chamber.
Turn OFF the power, and
Clean the transducer following
operators manual (Chap. Maint.).
5. Turn the main switch OFF, and wait for approx. 30 seconds.
Then disconnect the power cord.
Revised March 2006
Warning!
To avoid electrical shock, disconnect the power cord
before servicing.
(The main switch is not the disconnection device.)
13-15
6. Turn the 2 screws on the right side of the instrument

counterclockwise, using the Phillips screwdriver supplied with the
instrument. Then open the right side cover.
Revised March 2006
7. Grasp the handle of the detector cover to open it
13-16

8. Check that the fluid has been drained.
Plug
Warning!
Do not open the detector cover while the main switch is
ON. There is a risk of personal injury by electric shock.
Cloth
Aperture
If the fluid could not be drained completely and it remains in the

transducer, remove the fluid from the upper area of the transducer
(A), using the pipette supplied with the instrument.
9. Several drops of residual fluid will drip from the transducer chamber.
Therefore, place a cloth against the chamber when removing the
plug.
10. Apply CELLCLEAN to the transducer brush supplied with the
instrument, and clean the aperture by gently dabbing the aperture 5 to
6 times.
Important!
Rinse the brush with water, to remove CELLCLEAN
completely before storing it.
Warning!
should not come in contact with skin or clothing. If this
11. Replace the transducer chamber plug.
Revised March 2006
Caution!
Reattach the transducer chamber plug securely.
Otherwise, reagent leakage might occur, which
causes a short circuit or electrical shock.
13-17

12. Close the detector cover and the right side cover, and tighten the
screws of each. Then turn the main switch ON.
Be sure to turn the main switch ON upon

completion of this cleaning procedures.
If the instrument is left without auto rinsing for a
long time after cleaning of the transducer chamber
with CELLCLEAN, it may result in instrument
malfunction.
Revised March 2006
Caution!
Securely close the detector cover and the right
side cover. Failure to do so may result in damage
to the instrument.
13-18
13.11 Drain reagent

Drains all reagents in the instrument. Perform reagent draining when the
instrument is to be moved or shipped.
Biological risks
draining reagents. After completion of work, wash
hands with disinfectant.
1. Remove the caps from pocH-pack D and pocH-pack LVD. Pull the
container spout kits straight out to remove them.
Container
spout kits
pocH-pack D
or
pocH-pack LVD
Caution!
Place the removed container spout kits on top of a
clean cloth, or inside a plastic bag, and take care to
prevent dust or other substances from adhering to
them.
Ready
Menu
Main

ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Not Ready
Menu
Shutdown
Top

Chg.Reag
Calib.
Settings
Maint.
Revised May 2006
Str.Data
13-19

Not Ready
Top
Maint.
4. Press the [Drain Reagent] button.

The drain reagent starting screen will appear.
Auto Rinse
Status Display
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Setting Seq.
Clean W. Chamber
Drain Reagent
Not Ready
Top
Maint.

The drain reagent procedure from the instrument will be executed,
and the drain reagent screen will appear.
< Drain Reagent >
Drain reagent from the device,

and then, turn OFF the power.
When the [Cancel] button is pressed, reagent will not be drained.

And the screen returns to the Main screen.
Execute Cancel
Drain reagent.
Running
Maint.
Top
< Drain Reagent >
Please wait.
6. Check that the drain reagent completion screen is displayed, and turn
OFF the main switch of the instrument.
< Drain Reagent >
Reagent has been drained.
7. Remove the waste bottle cap. Pull the float switch straight out to
remove it.
Turn OFF the power.
Revised March 2006
Note:
The instrument will be primed automatically the next time
it is started up.
Also it can be primed by performing Setting Sequence in
the maintenance menu.
13-20
13.12 Calibrate LCD

The LCD screen of this instrument is a touch panel system.
This section explains the calibration procedures of the touch panel input
positions. Calibrate the LCD if panel operation is not properly aligned.
Ready
Menu
Main
Calibrate according to the following procedures.

ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu

Str.Data
Chg.Reag
Calib.
Settings
Maint.
Not Ready
Top
Maint.
3. Press the [Calibration LCD] button.

The calibration LCD start confirmation dialog will appear.
Auto Rinse
Status Display
Press the [OK] button to display the calibration LCD screen.
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Setting Seq.
Clean W. Chamber
Drain Reagent
4. Press the center of + mark on the screen each time the + mark is
displayed.
< Calibration LCD >
Revised May 2006
Press center of plus mark.

(total 5 times)
The + mark will appear on the LCD in the following order: at the
center, the upper left, the lower left, the lower right, and the upper
right. After completion of all + marks input, the calibration LCD
confirmation dialog screen will appear.
13-21
< Calibration LCD >
Press the [OK] button to apply the calibration, and the Main screen
will appear.

applying calibration.

(total 5 times)
OK
Calibration LCD?
Cancel
< Calibration LCD >

(total 5 times)
Quit
Calibration Error!
Note:
If the input position was outside a regulated range, the
calibration error message will appear, and the
calibration will be discontinued.
When calibration is performed and a calibration error
occurs frequently, there may be a problem with the
touch panel. Contact your Sysmex service
representative.
13.13 Setting Sequence

Primes the instrument with reagents. Perform this operation when
installing the instrument after being drained.
Ready
Menu
Main
ID
1. Confirm that the pocH-pack D and pocH-pack LVD are connected to

the instrument.
Species (1)Dog
Set a mixed
sample, and
Press [RUN].
RUN
select
Result
Shutdown
Revised May 2006
QC
13-22

Not Ready
Top
Menu

Str.Data
Chg.Reag
Calib.
Settings
Maint.
Not Ready
Top
Maint.
4. Press the [Setting Seq.] button.

The setting sequence starting screen will appear.
Auto Rinse
Status Display
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Setting Seq.
Clean W. Chamber
Drain Reagent
Not Ready
Top
Maint.

The sequence setting will start, the setting sequence screen will
appear, and the reagents will be filled.
< Setting Seq. >
Setting Seq.
Check tubes connecting reagents.
Press [execute] button.
When the [Cancel] button is pressed, the setting sequence stops,

and the screen returns to the Main screen.
Run setting sequence.
Execute Cancel
Running
Maint.
Note:
The instrument will not perform an Auto Rinse in the next
time it is started up after completion of the setting sequence
setting.
Top
< Setting Seq. >
Revised March 2006
Please wait.
13-23
13.14 Replace reagent

Biological risks
replacing reagents. After completion of work, wash
hands with disinfectant.
During the operation, instrument will automatically stop and an error

dialog will appear if a reagent runs out.
To replace the reagent, follow the procedure below.
Error dialog
Reagent to be replaced
Replace pocH-pack D
pocH-pack D
Replace pocH-pack LVD
pocH-pack LVD
1. Prepare a new reagent, and check the expiration date.
Caution!
Leave the reagents at room temperature (15 30C) for at least 24 hours or longer before using.
If a reagent that has arrived recently is used, a
correct analysis result will not be obtained.
Use the reagent at 15 - 30C. When analyzed with

reagent of a temperature higher than 30C or
lower than 15C, a correct analysis result will not
be obtained.
Always use new reagents.
Do not mix any left-over reagents with the new

reagents.
Do not allow the reagents to freeze.
Take care to prevent dust, dirt, bacteria, or other

materials from entering the reagent after it is
opened. If such materials enter, correct analysis
may not be possible.
Shelf life after opening

60 days
pocH-pack LVD
90 days
Revised May 2006
pocH-pack D
13-24

2. Remove the cap from the new reagent bottle.
Container
spout kits
3. Remove the cap from the empty reagent bottle. Pull the container
spout kit straight out to remove it.
4. Insert the container spout kit immediately straight into the new
reagent bottle, then tighten the cap.
pocH-pack D
or
pocH-pack LVD
Ready
Menu
Main
ID
Caution!
Take care not to touch the pipe that enters the
reagent with your hands, or to allow dust or other
substances to adhere to them. If such substances
adhere to the pipe, first wash the substance off with
reagent before attaching the container spout kit.
Failure to do so may prevent correct analysis.
Take sufficient care to prevent the reagent from
spilling. If the reagent spills, immediately wipe it off
with a damp cloth or similar material. The reagent
may cause discolor the floor.
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog

To execute from the error help screen, press the [Execute] button.
(See step 8.)
RUN
These screens show replacing pocH-pack D.
select
QC
Result
Shutdown
Not Ready
Top
Help
Replace pocH-pack D
pocH-pack D Error.
Replace container pocH-pack D.
[Exec.] Reaspirating pocH-pack D.
[Cancel] Back to the prev. screen.
ERROR CODE : XXXXX.XXXXX.XXXXX
Execute Cancel
Not Ready
Menu
6. Press the [Chg.Reag] button.

Top
The reagent information screen will appear.
Revised May 2006
Str.Data
Chg.Reag
Calib.
Settings
Press the [Top] button to return to the Main screen without

replacing the reagent.
Maint.
13-25

Not Ready
Top
Chg. Reag.
7. Press the [pocH-pack D] button to replace pocH-pack D.

Press the [pocH-pack LVD] button to replace pocH-pack LVD.
The reagent replacing screen will appear.
Choose the reagent to replace.

pocH-pack D
pocH-pack LVD
Not Ready
Top
Chg. Reag.
The reagent replacing dialog screen will appear, and reagent

aspiration will be performed.
< Replace pocH-pack D >
Replace the reagent , and

Press [Execute].

Aspriate reagents
Execute Cancel
Running
Maint.
When the [Cancel] button is pressed, reagent replacement is not

started, and the screen returns to the Main screen.
Note:
If the reagent is not replaced upon request, results may not
be obtained.
Top
< Replace pocH-pack D >
Revised March 2006
Please wait.
13-26
13.15 System fuse replacement

Warning!
To avoid electrical shock, disconnect supply before
servicing. (See Section 13.17 List of consumables)
1. Check that the main power switch is OFF. Unplug the power cable
from the top left of the instrument rear side.
2. Push the lugs of the both sides of the fuse holder inside, and remove
the fuse holder.
Warning!
replace only with a fuse of the specified type and
current ratings.
3. Replace the fuse(s). See Section 13.17 List of consumables.

4. Put the fuse holder back in place.
5. Plug in the power cable, and turn the main power switch ON.
Revised March 2006
Check that the instrument starts normally.
13-27
13.16 Replace thermal printer paper

When the paper of the built-in thermal printer has run out, an error
message No Printer Paper is displayed.
Follow the procedure below to replace the thermal printer paper.
1. Push down the lever on the right side of the built-in printer, then
open the printer cover.
Caution, Hot!
Push
Printer cover
Caution!
touch with hands.
2. Remove any remaining paper. Insert new printer paper, and close the
printer cover so that it catches the paper.
Important!
Set the paper straight and in the correct direction.
If inserted at an angle, the paper may become
jammed.
The printer cover must be closed securely until a
"click" sound is heard. If the cover is not closed
completely, an error will occur.
Revised March 2006
Thermal head
13-28

3. Cut the paper protruding out from the top of the built-in printer. (Cut
by pulling it around toward the right rear of the instrument.)
13.17 List of consumables

Note:
It is recommended to always keep a sufficient amount of
the following supplies and consumables available. This will
assure that your instrument is available for use.
Reagents
Product code
Product name
Purpose
024-0971-4
pocH-pack D
Diluent
054-1821-2
pocH-pack LVD
Lyse reagent
834-0162-1
CELLCLEAN(CL-50)
Detergent
Product code
Product name
Reference section
041-0031-5
Transducer Brush No.3
Section 13.10 Clean aperture of TD

(Transducer) chamber (Drain TD Chamber))
041-0061-7
CL6040 (5 rolls)
Section 13.16 Replace thermal printer paper
041-0051-0
Fuse 250V 3.15A H

Time Lag
Section 13.15 System fuse replacement
041-0041-2
WASTE BOTTLE
ASSY POCH
(C2/WITHOUT FSW)
Section 13.7 Dispose waste fluid
Revised May 2006
Consumables
13-29
Revised March 2006
13-30
Troubleshooting
14. Troubleshooting
If the instrument shows any signs that indicate a malfunction during
operation, be sure to first check 14.1 General faults, instrument failure
on the following pages. If the corresponding item is not found, or if the
procedure listed under Action does not eliminate the signs of trouble,
Other errors are indicated by a beep and a message is then displayed
on the LCD screen.
If an error affects only a specific analysis result, it will be marked by
a flag.
Warning!
Disconnect the power cord before opening the
instrument.
Otherwise there is a risk of personal injury by
electrical shock and possible damage to the
instrument.
Important!
If you are unable to rectify the error, contact your
Sysmex service representative for assistance. Before
doing so, make a note of the exact ERROR CODE in
the Help screen to enable your service representative
to provide assistance quickly.
Revised March 2006
Important!
In case of a power failure during operation, turn the
main power switch to [O] OFF position.
14-1
Troubleshooting
14.1 General faults, instrument failure

Trouble
Action
The instrument is switched ON

but will not start.
Check if power cold is plugged in properly.

Use another appliance to check if the outlet is live.
Check fuses and circuit breakers, replace if necessary
(see Section 13.15 System fuse replacement).
The logo appears on the LCD

screen, however the Main screen
does not appear.
The program card may have come out of the slot.

Turn the main power switch OFF, and check that the program card is
securely inserted in the card slot on the right side of the instrument.
Then turn the main switch ON again.
After turning the main switch ON,

the LCD screen remains blank.
A memory error may have occurred. Turn the main switch OFF, wait 1 to
2 minutes, then turn the main power switch ON again.
Mechanical operation is heard, but

no display appears on LCD screen.
Check that the LCD screen contrast is set correctly.
Fluid leaks from the instrument.
Turn the main power switch OFF and wipe off the leaked fluid.
Revised March 2006
Biological risks
Wear protective garments and gloves when working. After
completion of work, wash hands with disinfectant.
14-2
Troubleshooting
14.2 Error messages

General
Revised March 2006
When an error occurs, a beep alarm sounds, the

button flashes, and
an error dialog appears on the LCD screen.
Press the [OK] button to stop the alarm beep and close the error
dialog screen.
Press the
button to display the steps for rectifying the error on
the LCD screen.
If multiple errors occur simultaneously, they are displayed ranked by
importance.
Press the [Detail] button.
The help screen of the error at the top of the list will be displayed.
Follow the instructions.
Press the [Detail] button to go to the next screen.
14-3
Troubleshooting
(page)
Aperture Clog ..................................................................................... 14-9
Blank Error ......................................................................................... 14-9
Drain Error ......................................................................................... 14-8
HC ACK Timeout ............................................................................ 14-14
HC Buffer Full.................................................................................. 14-13
HC NAK Retry................................................................................. 14-14
HC OFF-line..................................................................................... 14-14
HGB Error ........................................................................................ 14-10
LAN Buffer Full............................................................................... 14-13
LAN no Response ............................................................................ 14-14
No Printer Paper ............................................................................... 14-13
Piercer MC Error ................................................................................ 14-8
Piercer MT Error ................................................................................ 14-8
PinchV1 MC Error ............................................................................. 14-8
PinchV2 MC Error ............................................................................. 14-8
PinchV1 MT Error.............................................................................. 14-8
PLT Noise Error ............................................................................... 14-12
PLT Smpg Error.............................................................................. 14-10
PPMC Cont. Error ............................................................................ 14-12
Print Buffer Ful ................................................................................ 14-13
Printer Error...................................................................................... 14-13
QC Data Error .................................................................................. 14-11
QC (L-J) Error .................................................................................. 14-10
QC (X) Error .................................................................................... 14-10
RAM Error ....................................................................................... 14-11
RBC Noise Error .............................................................................. 14-12
RBC Smpg Error............................................................................. 14-10
R-Cover Open................................................................................... 14-12
Replace pocH-pack D......................................................................... 14-7
Replace pocH-pack LVD ................................................................... 14-7
ROM Error ....................................................................................... 14-11
Room Temp. (H) ................................................................................ 14-9
Room Temp. (L)................................................................................. 14-9
Settings Error.................................................................................... 14-11
Settings Seq. Err ............................................................................... 14-12
Stored Error ...................................................................................... 14-11
Syringe MC Error............................................................................... 14-8
Syringe MT Error ............................................................................... 14-8
Tube holder Error. ............................................................................ 14-11
Vacuum Error ..................................................................................... 14-7
Waste C. Error.................................................................................... 14-7
WBC Noise Error ............................................................................. 14-12
WBC Smpg Error............................................................................ 14-10
14-4
Revised March 2006
Alphabetical error massage index
Troubleshooting
Error massage index by function
Pressure errors
(page)
Vacuum Error......................................................................................14-7
Chamber errors
Waste C. Error.....................................................................................14-7
Replace pocH-pack D .........................................................................14-7
Replace pocH-pack LVD ....................................................................14-7
Drain Error ..........................................................................................14-8
Motor errors
Syringe MC Error................................................................................14-8
Syringe MT Error ................................................................................14-8
Piercer MC Error.................................................................................14-8
Piercer MT Error .................................................................................14-8
PinchV1 MT Error ..............................................................................14-8
PinchV1 MC Error ..............................................................................14-8
PinchV2 MC Error ..............................................................................14-8
Transducer errors
Aperture Clog......................................................................................14-9
Temperature errors
Room Temp. (H) .................................................................................14-9
Room Temp. (L)..................................................................................14-9
Analysis errors
Blank Error..........................................................................................14-9
HGB Error .........................................................................................14-10
QC (L-J) Error...................................................................................14-10
PLT Smpg Error...............................................................................14-10
RBC Smpg Error..............................................................................14-10
WBC Smpg Error.............................................................................14-10
QC (X) Error .....................................................................................14-10
Revised March 2006
Memory errors
Stored Error .......................................................................................14-11
Settings Error ....................................................................................14-11
ROM Error ........................................................................................14-11
RAM Error ........................................................................................14-11
QC Data Error ...................................................................................14-11
14-5
Troubleshooting
Other errors
(page)
Tube holder Error ............................................................................. 14-11
Settings Seq. Err ............................................................................... 14-12
R-Cover Open................................................................................... 14-12
PLT Noise Error ............................................................................... 14-12
PPMC Cont. Error ............................................................................ 14-12
RBC Noise Error .............................................................................. 14-12
WBC Noise Error ............................................................................. 14-12
Printer errors
Print Buffer Ful ................................................................................ 14-13
No Printer Paper ............................................................................... 14-13
Printer Error...................................................................................... 14-13
External output errors
Revised March 2006
HC OFF-line..................................................................................... 14-14
HC NAK Retry................................................................................. 14-14
HC Buffer Full.................................................................................. 14-13
HC ACK Timeout ............................................................................ 14-14
LAN Buffer Full............................................................................... 14-13
LAN no Response ............................................................................ 14-14
14-6
Troubleshooting
14.3 Error messages, possible causes and actions to resolve the error
Vacuum Error
Possible cause
Defective vacuum pump or air leakage in vacuum line.
Action to resolve
the error
Special notes, if
error remains
Check if any fluid was collected in the trap chamber.

Press the [Execute] button to clear the error and perform the recovery process. If
the pressure returns to normal, then Auto Rinse and background check are
performed in sequence, and the screen returns to the Main screen. However, an
error occurs again if the pressure has not returned to normal.
Press [Cancel] to return to the previous LCD screen.
Not ready until the error is resolved.
Waste C. Error
Possible cause
Clogging of waste chamber and tubing.

Waste tubing is kinked.
Pinch valve 2 malfunctions.
Pinch valve 2 tubing is blocked.
Defective pressure pump or air leaking in pressure line.
Operation of float switch is abnormal.
Action to resolve
the error
Check the Clogging of waste chamber and tubing.

Check the kinked waste tubing.
After checking, press the [Execute] button to clear the error, and the waste
chamber drain sequence is executed.
Special notes, if
error remains
Revised March 2006
Replace pocH-pack D
Replace pocH-pack LVD
Possible cause
pocH-pack D or pocH-pack LVD is insufficient.
Action to resolve
the error
Special notes, if
error remains
Replace the pocH-pack D or pocH-pack LVD with new one.

Replace with a new reagent and press the [OK] button. The error is cleared and the
reagent replacement sequence is executed.
See Section 13.14 Replace reagent.
However, an error occurs again if the reagent replacement sequence is not
completed correctly.
14-7
Troubleshooting
Drain Error
Possible cause
The waste bottle is full.
Action to resolve
the error
Dispose waste fluid (see Chapter 13.7).

After checking, press [Execute] button to clear the error, and the recovery
sequence is executed.
Special notes, if
error remains
Piercer MC Error
PinchV1 MC Error
PinchV2 MC Error
Syringe MC Error
Possible cause
Abnormality in the controller for each driving motor.
Action to resolve
the error
Turn the main switch OFF, then ON again.
Special notes, if
error remains
Piercer MT Error
Syringe MT Error
Possible cause
Operation of each driving motor is abnormal.
Action to resolve
the error
Special notes, if
error remains
PinchV1 MT Error
Operation of driving motor is abnormal. The tube is stuck or broken.
Action to resolve
the error
Special notes, if
error remains
Revised March 2006
Possible cause
14-8
Troubleshooting
Aperture Clog
Possible cause
Transducer chamber aperture is clogged, or air bubbles have entered the transducer
chamber.
Action to resolve
the error
Special notes, if
error remains
Remove the aperture clog.

Execute automatic clog removal (see Chapter 13.9).
Clean the transducer (see Chapter 13.4).
Clean the aperture with the brush (see Chapter 13.10).
Press [Execute] button to clear the error, and the Clog Removal is executed.
Ready for next analysis, however, the next sample may be impaired.
Room Temp. (H)

Room Temp. (L)
Possible cause
Temperature in the detector block is too high or low.

Detector block temperature is 9C or lower: Room Temp (L)
Detector block temperature is 41C or higher: Room Temp (H)
Action to resolve
the error
Check to see that the room temperature is between 15-30 C.
Special notes, if
error remains
The * sign (indicates the result is low in reliability) will appear on the left side of
analysis results.
If the temperature in the detector block is too high or low, perform shutdown of the
instrument. Leave the instrument at an appropriate room temperature for some
time, then turn the main switch ON again.
Possible cause
Instrument is not operated for several days.

Aperture clogged.
Dirty HGB flow cell.
Air trapped in the system.
Reagent faulty.
Action to resolve
the error
Clean the transducer (see Chapter 13.4).

Replace the reagent (see Chapter 13.14).
Press [Execute] button to clear the error, and the Auto Rinse is executed.
Special notes, if
error remains
Revised March 2006
Blank Error
14-9
Troubleshooting
HGB Error
Possible cause
Action to resolve
the error
Press [Execute] button to clear the error, and the Clean Transducer is executed.
Special notes, if
error remains
Dirty HGB flow cell.

Bubbles mixed in HGB flow cell.
Dirty WBC Transducer chamber.
QC (L-J) Error
QC (X) Error
Possible cause
Aspiration of control blood was incomplete.

Mixing of control blood was insufficient.
Control blood was deteriorated.
Instrument failure.
TARGET value or LIMIT value was input wrong.
Action to resolve
the error
Reanalyze the control blood.

Confirm the TARGET value and LIMIT value.
Special notes, if
error remains
Ready for next analysis, however, the following sample results may be incorrect.
Possible cause
Aperture partly clogged.

Air bubbles sticking in transducer aperture.
Effect of external electric noise interference.
Action to resolve
the error
Remove the aperture clog.

Execute automatic clog removal (see Chapter 13.9).
Transducer cleaning sequence
Apply CELLCLEAN in the transducer, and clean by Transducer cleaning
sequence (see Chapter 13.4).
Cleaning aperture with transducer brush (see Chapter 13.10).
Separate the source of the electric noise from the instrument.
Press [Execute] to clear the error, and the Clog Removal is executed.
Special notes, if
error remains
14-10
Revised March 2006
PLT Smpg Error

RBC Smpg Error
WBC Smpg Error
Troubleshooting
QC Data Error
Setting Error
Stored Error
Possible cause
An error occurred in the set values of the stored data or QC data due to momentary
power failure, sudden electric noise interference, etc.
Action to resolve
the error
Special notes, if
error remains
Follow the instructions on the screen to repair the data. Then restart the
instrument.
If repair is not successful, then initialize the data.
When the instrument is initialized, all stored data is deleted. If settings have been
initialized, make settings again.
If either repair or initialize is successful, the program will be started.
RAM Error
ROM Error
Possible cause
CPU malfunction due to momentary power failure, sudden electric noise interference,
etc.
Action to resolve
the error
Special notes, if
error remains
Applications cannot be started.

Notifies the operator of the error by an alarm sound because it cannot be displayed on
the screen.
Tube holder Error

Possible cause
The sample position was opened while the instrument was operating.
Action to resolve
the error
After closing the door, press the [Execute] button to clear the error, and the recovery
sequence is executed.
Special notes, if
error remains
Revised March 2006
Setting Seq. Err

Possible cause
At the time the reagent was aspirated inside the instrument (priming), a motor error,
chamber error, sample position error, or right side cover error occurred.
Action to resolve
the error
Special notes, if
error remains
Press the [Execute] button, then turn OFF the main switch. Check the tubes
connecting the reagents and waste bottle, then restart the instrument.
Press the [Cancel] button to return to the previous LCD screen.
14-11
Troubleshooting
R-Cover open
Possible cause
The right side cover was opened.
Action to resolve
the error
After closing the right side cover, press the [Execute] button to clear the error, and the
recovery sequence is executed. If the recovery process is completed correctly, then
Auto Rinse and background check are performed in sequence, and the screen returns to
the Main screen.
Special notes, if
error remains
PLT Noise Error

RBC Noise Error
WBC Noise Error
Possible cause
Effects of external electric noise interference. Control board malfunction.
Action to resolve
the error
Separate the source of electric noise from the instrument.
Special notes, if
error remains
PPMC Cont. Error

Possible cause
Error in the motor controller circuit.
Action to resolve
the error
Special notes, if
error remains
HC Buffer Full
LAN Buffer Full
Print Buffer Ful
Possible cause
The amount of data for output or printout is too large to be processed.
Action to resolve
the error
Special notes, if
error remains
14-12
Revised March 2006
Press [Back] button to clear the error.
Troubleshooting
No Printer Paper
Possible cause
No printer paper in the built-in thermal printer.
Action to resolve
the error
Special notes, if
error remains
After refilling paper in the built-in thermal printer, press [Retry] button to clear the
error, and the data under printing will be printed from the beginning by one sample
(see Chapter 13.16).
After refilling paper in the built-in thermal printer, press [Cancel] button to cancel
the data under printing, also discard the waiting data, and the error is cleared.
Printer Error
Possible cause
The built-in thermal printer has an error.

The printer cover is open.
Action to resolve
the error
Check if the paper is set correctly and close the cover (see Chapter 13.16).
After checking the built-in thermal printer, press [Retry] button to clear the error,
and the data under printing will be printed from the beginning by one sample.
After checking the built-in thermal printer, press [Cancel] button to cancel the data
under printing, also discard the waiting data, and the error is cleared.
Special notes, if
error remains
HC ACK Timeout
HC Off-line
HC NAK Retry
Possible cause
Computer connecting cable failure.

Computer main switch is not turned on, or computer not ready for communication.
Host computer serial interface error.
Action to resolve
the error
Inspect the host computer cable.

Inspect the connection cable to host.
Press [Retry] button to clear the error, and the transmission to the host computer
is restarted.
Press [Cancel] button to clear the error, and all the output queued strings to host
computer are deleted.

If the retry action does not resolve the problem, select the off-line in order to make the
instrument in ready mode.
Revised March 2006
Special notes, if
error remains
14-13
Troubleshooting
LAN no Response
Computer connecting cable failure.

Computer main switch not turned on, or computer not ready for communication.
LAN connector for host computer failure.
Action to resolve
the error
Check the host computer cable.

Check the connection cable to host.
Press [Retry] button to clear the error, and the transmission to LAN is restarted.
Press the [Cancel] button to clear the error. All data for LAN output is deleted.
Special notes, if
error remains

If the retry action does not resolve the problem, select the off-line in order to make the
instrument in ready mode.
Revised March 2006
Possible cause
14-14
Troubleshooting
14.4 Print error log

An overview of the latest 10 error messages can be printed out on the
built-in thermal printer.
Note:
Have the error messages printed out before contacting your
Sysmex service representative.
Ready
Menu
Main
ID

1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
QC
Result
Shutdown
Not Ready
Top
Menu
Str.Data
Chg.Reag
Calib.
Maint.
Settings
Not Ready
Top
Maint.
3. Select the [Print Error Log] button.

The built-in thermal printer will print out the error history.
Auto Rinse
Status Display
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Drain Reagent
Revised May 2006
Clean W. Chamber
14-15
Revised March 2006
Troubleshooting
14-16
Technical Information
15. Technical Information

15.1 Performance characteristics/Specifications
Conditions
Ambient temperature
Operating
Storage and
Transportation*
+15C to +30C
(+23C would be ideal)
-10C to + 60C
30% to 85%
95% or less
(Non condensing/Keep dry)
Relative humidity
Main Unit
dimensions
Width:185 mm (7.3 in)

Depth:460 mm (18.1 in)
Height:350 mm (13.8 in)
Weight:approx. 14 kg (30.8 lbs)
Power supply
100 to 240 VAC 10% (50/60 Hz)
Power consumption
150 VA or less
Analysis Parameters
see Chapter 1.3 Abbreviations and units used throughout this manual
Display range
WBC 0 - 999.9 ( 103/L)

RBC 0 - 99.99 ( 106/L)
HGB 0 .0 - 999.9 (g/dL)
PLT
Background limits
0.0 - 9999 ( 103/L)
WBC 0.3 (103/L)

RBC 0.02 ( 106/L)
HGB 0.1 (g/dL)
PLT
10 ( 103/L)
Analysis time
Approx. 125 seconds (After starting an analysis until displaying the analysis report)
Analysis principle
WBC: DC detection method

RBC/PLT: Hydrodynamic Focusing DC detection method
Revised March 2006
HGB: Non-cyanide HGB method

Required temperature
compensation
Approx. 512 BTU/h (Approx. 130 kcal/h)
Class of electric
shock protection
measures
Class I Equipment
EMC characteristics
Conforms with IEC 61326-1 (Class B, Group 1, Industrial environment)
* You have to perform Reagent Draining in prior to the storage or transportation.

15-1
Safety
Conforms with IEC 61010-1

(Overvoltage Category II, Pollution degree 2, Portable equipment)
Reproducibility
(With 95% reliability
limit)
WBC ( 4.0 103/L)

RBC ( 3.00
/L)
HGB
HCT
MCV
MCH
MCHC
PLT ( 100 103/L)
(for dog, cattle or horse
blood)
PLT ( 100 103/L)
(for cat blood)
106
LYM#
30.0% or less
30.0% or less
OTHR#
EO# ( 0.2
3.5% or less
2.0% or less
2.0% or less
2.0% or less
2.0% or less
2.0% or less
2.0% or less
10.0% or less
15.0% or less
103/L)
50.0% or less
30.0% or less
OTHR%
15.0% or less
EO% ( EO 2%)
50.0% or less
RDW-SD
4.0% or less
RDW-CV
6.0% or less
PDW(for dog, cattle or

horse blood)
12.0% or less
MPV (for dog, cattle or

horse blood)
5.0% or less
P-LCR (for dog, cattle or

horse blood)
20.0% or less
Revised May 2006
LYM% ( LYM 15%)
15-2
Linearity
1.0 - 99.9 (103/L)
0.3 (103/L) or less, or

5% or less
1.0 - 75.0 (103/L)
0.3 (103/L) or less, or

5% or less
RBC
0.3 - 13.00 (106/L)
0.03 (106/L) or less, or

5% or less
HGB
0.1 - 25.0 (g/dL)
0.2 (g/dL) or less, or

5% or less
HCT
10.0 - 60.0 (HCT%)
1 (HCT%) or less, or
5% or less
PLT
(RBC < 7.00 106/L)
10 - 1200 (103/L)
10 (103/L) or less, or
10% or less
WBC
(RBC < 7.00 106/L)
(for dog, cattle or horse
blood)
WBC
(RBC < 7.00 106/L)
(for cat blood)
Carry-over
WBC
RBC
HGB
HCT
PLT
Consumables
Reagents: pocH-pack D, pocH-pack LVD

Detergent: CELLCLEAN
Control material: EIGHTCHECK-3WP
Aspirated sample
volume
approx. 15 L
Number of analyses
that can be
performed with 1
reagent bottle
pocH-pack D: Approximately 30
3% or less
1.5% or less
1.5% or less
1.5% or less
5% or less
pocH-pack LVD: Approximately 235

(These figures assume 10 measurements per day, and include the background check,
shutdown, and other processes.)
Revised May 2006
Note:
The abnormal sample conditions listed here are known to affect test results. The majority of
the listed sample conditions are not measured quantitatively because these conditions vary
due to animal species, diagnosis, age, medications, etc. Laboratories can perform studies in
order to show how their specific animal species are affected by various conditions.
15-3
15.2 System limitations - interferences

WBC: false high white blood cell count
Cause:
Potential Detection:
Lyse resistant red blood cells
Abnormal WBC histogram (WL flag)
Cold agglutinins
Increased MCV, increased MCHC due to decreased

HCT and RBC
Platelet aggregation / cryoglobulin
Abnormal WBC histogram (WL flag) and Abnormal

PLT histogram (PU flag)
Nucleated red blood cells
Abnormal WBC histogram (WL flag)
RBC: false low red blood cell count

Cause:
Cold agglutinins
Increased MCV, increased MCHC due to decreased

HCT
Microcytosis (severe)
Low MCV
Fragmented red blood cells
Abnormal RBC histogram (RL flag) and Abnormal

HCT: false low haematocrit measurement

Cause:
Cold agglutinins
Increased MCV and increased MCHC
Abnormal RBC histogram (RL flag) and abnormal

HCT: false high haematocrit measurement

Cause:
Leukocytosis
Very high white blood cell count with low red blood
cell count present
PLT: false low platelet count

Cause:
Platelet aggregation
Abnormal PLT histogram (PU flag)
Giant platelets
Abnormal PLT histogram (PU flag)
15-4
Cause:
Microcytic red blood cells
Low MCV
Abnormal RBC histogram (RL flag) and abnormal

Revised May 2006
PLT: false high platelet count
15.3 Print formats

Output on built-in thermal printer
Shown are examples of printed results on the thermal built-in printer. For the setting procedures, see
Section 12.2 Possible settings.
Type 1
Species
(1)Dog
Manual Ana.
[S][W][R][P]
Species
Type 3
Species
(1)Dog
Manual Ana.
[S][W][R][P]
(1)Dog
Manual Ana.
[S][W][R][P]
LYM%
OTHR%
EO%
LYM#
OTHR#
EO#
Revised March 2006
LYM%
OTHR%
EO%
LYM#
OTHR#
EO#
Type 2
15-5
15.4 Connecting the bar code reader

A bar code reader is available as an option for the pocH-100iV Diff.
Part No.
Part name
Explanation
281-9576-1
TOUCH65-PRO
Bar code reader unit
265-9151-5
CAB320-DIN8
Connects the bar code reader to the

instrument.
281-9577-5
SPC-Touch 65/90
Desk Holder
Stand for the bar code reader
Revised March 2006
No.
15-6
Connection procedure
1. Turn the main switch OFF.
2. Insert the connection cable into the bar code reader unit.
3. Insert the other end of the connection cable into the connector port
labeled BR on the right side of the instrument.
4. Turn the main switch ON.
Revised March 2006
Press the trigger switch and check that the red LED is ON.
Trigger switch
15-7
Bar code reader settings
Make settings according to the menu sheet which is provided with the bar code reader.
Communications parameters were set at the time the unit was shipped from the factory. They do not need
to be set.
Make settings to match the bar code label which you use.
Set the ID character and check digit to Not transmit.
Hardware specifications
1. Symbologies which can be read: UPC-A/E, CODE39, CODE128, ITF, NW-7 (CODABAR)
2. Decoder: Built-in
3. Interface: RS-232C
4. Connector: DIN 8P
5. Power input: 5 V DC 5%
6. Connector pin arrangement
No.
Signal name
Signal direction
TxD (Transmit Data)
Reader Analyzer
RxD (Receive Data)
Reader Analyzer
RTS (Request To Send)
Reader Analyzer
CTS (Clear To Send)
Reader Analyzer
NC (Not Connected)
DTR (Data Terminal Ready)
Reader Analyzer
SG (0 V)
Reader Analyzer
+5 V (+5 V)
Reader Analyzer
Software
1. Data communication protocol
E
T
X
Order of transmission
15-8
Revised March 2006
S
T
X
2. Data transmission parameters
Parameter
Setting value
Baud rate
9600 bps
Data length
8 bits
Stop bit
1 bit
Parity
None
RTS/CTS
Valid
Transfer sequence
No protocol
Check digit
Not transmit
ID character
Not transmit
Preamble
STX (02H)
Postamble
ETX (03H)
ID Bar code Specifications

When using bar code labels, labels with specifications that match the bar code reader must be used.
This section explains the bar code label specifications.
Note:
Following is an explanation of the typical bar code label specifications. The actual
specifications may differ depending on the symbology, print quality, and number of digits.
Specifications for JAN codes will also be somewhat different. For details, contact the
Sysmex service representative.
1. Symbologies used
The symbologies and check digits which can be used are listed below.
(1) Sample ID numbers
Warning!
If the sample bar code is used, use a check digit whenever possible.
If a check digit is not used, the chances of the bar code being misread increase.
Symbology
ITF
Check digit
Number of digits
None
Max. 14 digits (sample ID)
Modulus 10
Revised March 2006
+ 1 digit (check digit) = Max. 15 digits

NW-7
None
Modulus 11
Weighted modulus 11
Modulus 16
15-9
Symbology
Check digit
CODE39
Number of digits
None
Modulus 43

CODE128
Modulus 103

JAN-8
Modulus 10
7 digits (sample ID)

+ 1 digit (check digit) = 8 digits
JAN-13
Modulus 10
12 digits (sample ID)

+ 1 digit (check digit) = 13 digits
2. Bar code element dimensions

Narrow:
Min. 0.20 mm
Narrow: Wide
2.2 - 3.0
Narrow space: Narrow bar
0.85 - 1.17
3. PCS (Print Contrast Signal) value

(Reflectivity of the space - Reflectivity of the black inked bar)
PSC =
Reflectivity of the space
Standard: PCS value 0.45
The analysis method conforms to JIS (Japanese Industrial Standards) X0501, Section 5.3: Optical
Characteristics of Bar code Symbols.
Standard: PCS value 0.45
4. Surface reflection
A laminated label cannot be read.
5. Irregularity and Roughness of Printing
Enlarging a single bar from a bar code with a microscope shows something like the figure below.
MAX
MIN
Bar element
S=
MAX - MIN
100%
MAX
Then the variation coefficient (S) must be 20% or less.
15-10
Revised March 2006
When the variation coefficient (S) in the width of a bar is defined:
6. Bar code label dimensions
Bar height
Space Effective bar code width Space
Space:
Min. 2.5 mm or 10 largest module value
Effective bar code width: Max. 48 mm (Optimum: 40 mm or less)
Bar height:
Min. 20 mm
7. Check digit
A check digit can be added to further increase the reliability of the ID number which is read.
Following is an example of the methods used to calculate the check digit of modulus 11 and weighted
modulus 11 for a sample ID number of 258416.
1) Modulus 11
(1) A weight is assigned to each digit.
Weight
14
30
40
16
12
(2) The results of multiplication are all added together. The result is S.
S = 14 + 30 + 40 +16 + 3 + 12 = 115
(3) A remainder is found by dividing S by 11. The complement of this remainder is then found.
The 11-complement becomes the check digit value to be added.
115 / 11 = 10 (remainder = 5)
11 - 5 = 6 The check digit is 6.
All alphabet and symbol characters other than numbers 0 to 9 are considered for calculation to be 0.
If the remainder is 0 when S is divided by 11, or if the result of check-digit calculation is 10, then the
check digit is 0.
2) Weighted modulus 11
With weighted modulus 11, there are two sets of weights for each digit. The check is performed first
using the first set of weights. If the result is 10, then the check is performed again using the second set
of digits. The result is assured to be 0 to 9. Except for the difference in weights, the calculation
method is exactly the same as for modulus 11.
Revised March 2006
(1) Weights are assigned to each digit.

Weight:
W12
W11
W10
W9
W8
W7
W6
W5
W4
W3
W2
W1
First Set:
10
Second Set:
10
15-11
Weight
16
20
40
12
12
(2) The results of multiplication are all added together. The result is S.
S = 16 + 20 + 40 + 12 + 6 + 12 = 106
(3) A remainder is found by dividing S by 11. The complement of this remainder is then found.
The 11-complement becomes the check digit value to be added.
106 / 11 = 9 (remainder = 7)
11 - 7 = 4 The check digit is 4.
All alphabet and symbol characters other than numbers 0 to 9 are considered for calculation to be 0.
If the remainder is 0 when S is divided by 11, or if the result of check-digit calculation is 0, then the
check digit is 0.
Revised March 2006
Note:
The weight of digits 13 to 15 is 0.
15-12
15.5 Interface protocol

Data output can be made in different formats service via the serial interface or the Ethernet.
15.6 Output format for host computer

Serial (RS-232C standard) and LAN (Ethernet) output can be used for output from this instrument to the
host computer.
Data is output from the interface ports labeled
Connect one of these ports to the host computer.
or
on the right side of the main unit.
Note:
To utilize the host computer output interface, install software compatible with
pocH-100iV Diff onto the computer to enable data reception.
Serial communications
Hardware
(1) Connector
Connect to the connector labeled
The instrument connector is a 9-pin D-SUB male connector.

Connect a 9-pin D-SUB female connector to the instrument.
Fixing screws for this connector are in inch-specification.
(2) Instrument connector signal

Pin No.
Signal name
Signal direction
1
2
Receive Data
(RxD)
From Host to pocH-100iV Diff
Transmit Data
(TxD)
To Host from pocH-100iV Diff
Data Terminal Ready
(DTR)
Signal Ground
(SG)
Data Set Ready
(DSR)
Request To Send
(RTS)
Clear To Send
(CTS)
Revised March 2006
15-13
(3) Communication format
Asynchronous full duplex mode is used. (The underlined items are initial settings.)
Setting
Setting type
1200 bps / 2400 bps / 4800 bps / 9600 bps / 19200 bps
Baud rate
Data length
7 bits / 8 bits
Stop bit
1 bit / 2 bits
Parity
Even / Odd / None
Protocol
Class A / Class B
Interval
0 s / 2 s / 3 s / 5 s / 7 s / 10 s / 15 s
RTS/CTS
Use / Not use
(4) Signal level

Conforms to EIA RS-232C V.24. as shown below.
Level
Data signal
Control signal
+3 V or more
Logic 0, Start bit
ON
-3 V or less
Logic 1, Stop bit
OFF
Software
(1) Communication format
1)
Code
Data: ASCII code
Control code: [STX], [ETX], [ACK], [NAK]
2)
Text structure
[STX] is sent at the start of the data, and [ETX] is sent at the end of the data.
S
T
X
E
T
X
3)
Communication protocol
Class A
This is unidirectional transmission which does not utilize responses from the host
computer.
15-14
Revised March 2006
There are two classes of communication protocols that can be selected according to the system
status.
Class B
Confirmation responses ([ACK], [NAK]) are received from the host computer in response
to the transmitted data. Data is transmitted alternately between the instrument and host
computer.
Host computer
pocH-100iV Diff
Analysis data is received.
Analysis data is sent.

Analysis data transmission
is completed correctly.
[ACK]
Repeats transmission a
maximum of 3 times. If [NAK] is
received in response to the third
transmission, the operator uses
the touch panel to select whether
to retransmit the same data or
cancel transmission.
4)
[NAK]
When an error does not

occur, [ACK] (06H) is sent.
When an error occurs, [NAK]
(15H) is sent.
Transmission errors
If an error occurs during transmission, transmission is interrupted and an error message is
displayed. The operator can select whether to retransmit ([OK] button) or cancel transmission
([CANCEL] button). A transmission error occurs in the following cases.
Host computer is off-line.
Control signal DSR is OFF.
There is no response from the host computer within 15 seconds after data is sent (Class B
only).
The host computer sends anything other than [ACK] or [NAK] after data is sent (Class B
only).
[NAK] is sent in response to the fourth transmission of the data (Class B only). (The
instrument automatically repeats data transmission a maximum of 3 times when it receives
[NAK].)
5)
Transmission timing, destination, and contents

Serial interface
Revised March 2006
Host computer
In normal analysis
Analysis data (A)
In QC analysis
QC data (A)
Analysis data includes the date, sample number, analysis data, flags, and other information.
QC data is X or L-J data.
Settings for connected devices are changed in the setting program.
15-15
LAN communication
Hardware
The physical layer and data link layer of this port conform to IEEE802.3.
1. Connector
Connect to the connector labeled
The instrument connector utilizes a twisted-pair cable (UTP cable category 5) for Ethernet use.
(Connector: RJ-45)
2. Connector signals
Pin No.
Signal name
Signal direction
TD+
TD-
RD+
NC
NC
RD-
NC
NC
Software
1. Network layer/ transport layer
Conforms to the TCP/IP protocol.
2. Session layer
The connection is made with the host computer as the server and the instrument as the client.
They are connected at start-up.
If the connection fails, a connection error occurs. The connection is attempted again after the
operator has corrected the error using the touch panel.
3. Presentation layer
ASCII code is used for the transmission data. [STX] is added to the start of the data, and [ETX] is
added to the end of the data.
E
T
X
15-16
Revised March 2006
S
T
X
Format
The same format is output through serial or LAN port, and includes 4 types of output formats:
pocH format
KX-21N format
ASTM format
K-1000 format
pocH format
pocH format includes an Analysis data format for output of sample data, and a QC data format for
output of QC data. Each has different text length and contents. (They are identified by the sample
distinction code.)
If the length of the transmitted data, including [STX] and [ETX], exceeds 256 bytes, it is divided into
multiple text blocks for transmission. The divided text blocks are sent in order, beginning from the head,
and no other data is mixed in between the blocks. The text sequence is expressed by text distinction code
II numerals.
Analysis data format
Revised March 2006
Parameter
No. of characters
Format
STX
(02H)
Text distinction code I
Fixed character: D
Text distinction code II
Text No.: 1
Sample distinction code
Fixed character: U
Instrument ID
40
(Instrument name)^(PS code)^(Serial No.)

40 characters, left-justified, with space padding.
Example:
pocH-100iV Diff^12345678^12345678901245
Year
4 digits, with zero padding. Example: 2001
Month
Day
Analysis information
Sample ID
15
Instrument ID, 15 characters, right-justified, with space

padding or zero padding (as set in ID Pad. of Host output
settings).
Example:
AB-12345, 0000000AB-12345
Particle distribution
analysis information
Species
A - Q
Example: Dog:A, Cat:B, OTHER10:J
WBC
4 significant digits, with decimal point excluded, with zero

padding + 1 digit of flag information.
Example:
Data (76), Flag (3) 00763
15-17
Parameter
No. of characters
Format
RBC

Example:
Data (76), Flag (3) 00763
HGB
Same as above
HCT
Same as above
MCV
Same as above
MCH
Same as above
MCHC
Same as above
PLT
Same as above
LYM% (W-SCR)
Same as above
OTHR% (W-MCR)
Same as above
EO% (W-LCR)
Same as above
LYM# (W-SCC)
Same as above
OTHR# (W-MCC)
Same as above
EO# (W-LCC)
Same as above
RDW-SD
Same as above
RDW-CV
Same as above
PDW
Same as above
MPV
Same as above
P-LCR
Same as above
ETX
(03H)
Total 171
Parameter
No. of characters
Format
STX
(02H)
Fixed character: D
Text No.: 2
WBC particle size data
100
50 sets of 2-digit hexadecimal data, converted to ASCII
RBC particle size data
100
ETX
(03H)
Total 204
15-18
No. of characters
Format
STX
(02H)
Fixed character: D
Text No.: 3
PLT particle size data
80
Revised March 2006
Parameter
Parameter
No. of characters
Format
WBC LD
WBC T1
2 sets of 2-digit hexadecimal data, converted to ASCII.
WBC T2
WBC UD
RBC LD
Same as above
RBC UD
Same as above
PLT LD
Same as above
PLT UD
Same as above
RESERVED
128
ETX
128 spaces (20H)

(03H)
Total 228
QC data format
Revised March 2006
Parameter
No. of characters
Format
STX
(02H)
Fixed character: D
Text No.: 1
Fixed character: C
Instrument ID
40
(Instrument name)^(PS code)^(Serial No.)

40 characters, left-justified, with space padding.
Example:
pocH-100iV Diff^12345678^12345678901245
Lot ID
10
10 characters, left-justified, with space padding
Data distinction code
QC method
X control: X, L-J control: L
Year
Month
Day
Hour
Minute
Data ID
1 digit QC file No. Example: 1
RESERVED
RESERVED
WBC
4 significant digits, with decimal point excluded, with

zero padding.
Example:
Data (76) 0076
LYM%
Same as above
OTHR%
Same as above
15-19
Parameter
No. of characters
Format
EO%
Same as above
LYM#
Same as above
OTHR#
Same as above
EO#
Same as above
RBC
Same as above
HGB
Same as above
HCT
Same as above
MCV
Same as above
MCH
Same as above
MCHC
Same as above
RDW-SD
Same as above
RDW-CV
Same as above
PLT
Same as above
PDW
Same as above
MPV
Same as above
P-LCR
Same as above
W-SMV
Same as above
W-LMV
Same as above
ETX
(03H)
Total 155
Parameter
No. of characters
Format
STX
(02H)
Fixed character: D
Text No.: 2
WBC particle size data
100
RBC particle size data
100
ETX
(03H)
Total 204
15-20
No. of characters
Format
STX
(02H)
Fixed character: D
Text No.: 3
PLT particle size data
80
WBC LD
WBC T1
Revised March 2006
Parameter
Parameter
No. of characters
Format
WBC T2
WBC UD
RBC LD
Same as above
RBC UD
Same as above
PLT LD
Same as above
PLT UD
Same as above
RESERVED
128
ETX
128 spaces (20H)
(03H)
Total 228
Details of pocH format

Instrument ID and particle size data are added to KX-21N format. For QC format, lot information is
added also. For other data, see the description of KX-21N format.
Histogram data and discriminator values
Histogram data is 2-digit hexadecimal data converted to ASCII. It is 50 bins of data for WBC and
RBC, and 40 bins of data for PLT. Discriminator values are "WBC LD," "WBC UD," "RBC LD,"
"RBC UD," "PLT LD," and "PLT UD." Like the histogram data, the discrete values are also 2-digit
hexadecimal data converted to ASCII.
Example:
ch[0]=123(0x7B)
ch[1]=45(0x2D)
ch[2]=56(0x38)
ch[49]=67(0x43)
The above data is arranged as shown below.
7B2D38...........................43
Instrument ID
Instrument ID is composed of [(Instrument name)^(PS code)]. It is a character string of 40 characters,
left-justified, with space padding. The PS code is fixed according to the instrument specifications.
Example:
Instrument name: pocH-100iV Diff
PS code: 1234
Revised March 2006
Serial No.: 123456 (serial number set at user information settings)
pocH-100iV Diff^1234^123456
15-21
KX-21N format
KX-21N format includes an Analysis data format for output of sample data, and a QC data format for
distinction code.)
Text distinction code II is usually 1.
However, if the text length becomes 256 bytes or more, the text will be divided into 2 or more blocks for
transmission. The sequence of these blocks will be expressed by the text distinction code II numerals.
([ETB] codes will not be used.)
15-22
No. of characters
Format
STX
(02H)
Fixed character: D
Text No.: 1
Fixed character: U
Year
Month
Day
Sample ID
15

padding or zero padding (as set in ID Pad of Host output
settings).
Example:
AB-12345
Particle distribution
analysis information
Species
A - Q
Example: Dog:A, Cat:B, OTHER10:J
WBC

Example:
Data (76), Flag (3) 00763
RBC
Same as above
HGB
Same as above
HCT
Same as above
MCV
Same as above
MCH
Same as above
MCHC
Same as above
PLT
Same as above
LYM% (W-SCR)
Same as above
OTHR% (W-MCR)
Same as above
EO% (W-LCR)
Same as above
Revised March 2006
Parameter
Parameter
No. of characters
Format
LYM# (W-SCC)
Same as above
OTHR# (W-MCC)
Same as above
EO# (W-LCC)
Same as above
RDW-SD
Same as above
RDW-CV
Same as above
PDW
Same as above
MPV
Same as above
P-LCR
Same as above
ETX
(03H)
Total 131
QC data format
Revised March 2006
Parameter
No. of characters
Format
STX
(02H)
Fixed character: D
Text No.: 1
Fixed character: C
QC method
X control: X, L-J control: L
Year
Month
Day
Hour
Minute
Data ID
RESERVED
RESERVED
WBC

zero padding.
Example:
Data (76) 0076
LYM%
Same as above
OTHR%
Same as above
EO%
Same as above
LYM#
Same as above
OTHR#
Same as above
EO#
Same as above
RBC
Same as above
15-23
Parameter
No. of characters
Format
HGB
Same as above
HCT
Same as above
MCV
Same as above
MCH
Same as above
MCHC
Same as above
RDW-SD
Same as above
RDW-CV
Same as above
PLT
Same as above
PDW
Same as above
MPV
Same as above
P-LCR
Same as above
W-SMV
Same as above
W-LMV
Same as above
ETX
(03H)
Total 105
Details of KX-21N format

Sample ID
If the sample ID is less than 15 digits in length, it is extended to 15 digits by right-justified space padding
or zero padding, according to the ID Pad. setting in HC output settings.
Example:
Space padding: 12345
12345
Zero padding: 12345000000000012345

Position of decimal point
The position of the decimal point differs depending on the units. The decimal point is not output to the
host computer, and only 4 significant digits are output. The significant digits are the same as for screen
display, and do not depend on the units. See the external specifications. The output sequence is as shown
from top to bottom in the above table. Data is sent, with zero padding, from the most significant digit.
Date format
Revised March 2006
The year-month-day sequence for the date is fixed, regardless of the date format set for the system
settings.
15-24
The format of the analysis information is fixed at zero (0), and cannot be changed.
Particle distribution analysis information
The particle distribution analysis information is composed of the following 5 elements.
1)
Parameter
No. of characters
WBC histogram information
WBC histogram flag
RBC histogram information
RBC histogram flag
PLT histogram information
PLT histogram flag
Histogram information
Histogram information contains the following information.
2)
Code
Explanation
Normal histogram
Abnormal histogram
Manually discriminated
Histogram flag
The histogram flags are composed of the following 10 types. Each type corresponds to a
flagging character for the LCD screen and printer.
The histogram flag is 0 for a normal histogram and is 1 - 2 for a histogram error.
Relationship of histogram flags to flagging characters
Revised March 2006
Histogram flag
Flagging character at LCD screen

and printer, and flagging parameter
at HC
Remarks
WBC
RBC
PLT
Normal
Normal
Normal
WL
RL
PL
High frequency at
L discriminator (WBC/RBC/PLT)
WU
RL
PU
High frequency at
H discriminator (WBC/RBC/PLT)
DW
DW
Cannot be analyzed because there is

not an intersection point at the
frequency level of 20% for DW
calculation. (RBC/PLT)
MP
MP
Multiple peaks (RBC/PLT)
T1
Normal (WBC/RBC/PLT)
T1 discriminator cannot be
determined. (WBC)
15-25
Histogram flag

at HC
WBC
RBC
Remarks
PLT
T2
determined. (WBC)
F1, F2
T1 relative frequency exceeded

specified value. (WBC)
F2, F3
Reliability of the data is low due to

high frequency level at T1 or T2
discriminator. (WBC)
Species
pocH outputs the analysis results with following species information.
Code Mode / Species
"0"
Background
"A"
(1) Dog
"B"
(2) Cat
"C"
(3) Cattle
"D"
(4) Horse
"E"
(5) OTHER1
"F"
(6) OTHER2
"G"
(7) OTHER3
"H"
(8) OTHER4
"I"
(9) OTHER5
"J"
(10) OTHER6
"K"
(11) OTHER7
"L"
(12) OTHER8
"N"
(14) OTHER10
"O"
(15) OTHER11
"P"
(16) OTHER12
"Q"
(17) OTHER13
Caution!
The analysis data of the code"E" - "Q" is for research.
15-26
Revised May 2006
"M" (13) OTHER9
Numerical data
Numerical data is composed of the following.
The data is 4 significant digits, with the decimal point excluded. It is output without zero-suppression.
Upper digit
Data
Lower digit
Flag
Flag details
Code
Explanation
Normal
Judgment on reference limits: +
Judgment on reference limits: -
Out of linearity limits
Low reliability
Data masking
In the case of data overflow, the data is changed to the following.

*0003
In the case of an analysis error, or analysis parameters with no data , the data is changed to the
following.
*0000
In the Cat mode, the data of PDW, MPV and P-LCR are output as 5 spaces (20H).
In the Cattle or Horse mode, the data of EO% and EO# are output as 5 spaces (20H).
Data ID
Revised May 2006
Data ID for the QC format is the QC file number. It is output as a single character, from 1 to 3.
15-27
K-1000 format
K-1000 format includes an Analysis data format for output of sample data, and a QC data format for
distinction code.)
The format for text distinction code II is normally 1.
If the text length exceeds 256 bytes when a modification is made in the future, it will be divided into two
or more blocks for transmission, and the sequence of blocks will be identified by the numerals of the text
distinction code II. (ETB code is not used.)
15-28
No. of characters
Format
STX
(02H)
Fixed character: D
Text No.: 1
Fixed character: U
Year
Month
Day
Sample ID
12

padding or zero padding (as set in ID Pad of Host
output settings).
Example:
AB-12345
Particle distribution analysis

information
RDW switching information
S or C
RDW-CV: C, RDW-SD: S
WBC

zero padding + 1 digit of flag information.
Example:
Data (76), Flag (3) 00763
RBC
Same as above
HGB
Same as above
HCT
Same as above
MCV
Same as above
MCH
Same as above
MCHC
Same as above
PLT
Same as above
Revised March 2006
Parameter
Parameter
No. of characters
Format
LYM% (W-SCR)
Same as above
OTHR% (W-MCR)
Same as above
EO% (W-LCR)
Same as above
LYM# (W-SCC)
Same as above
OTHR# (W-MCC)
Same as above
EO# (W-LCC)
Same as above
RDW-SD or RDW-CV
Same as above
PDW
Same as above
MPV
Same as above
P-LCR
Same as above
ETX
(03H)
Total 121
QC data format
Revised March 2006
Parameter
No. of characters
Format
STX
(02H)
Fixed character: D
Text No.: 1
Fixed character: C
X or L
Year
Month
Day
Hour
Minute
Data ID
RESERVED
S or C
RDW-CV: C, RDW-SD: S
WBC

zero padding.
Example:
Data (76) 0076
LYM%
Same as above
OTHR%
Same as above
EO%
Same as above
LYM#
Same as above
OTHR#
Same as above
EO#
Same as above
15-29
Parameter
No. of characters
Format
RBC
Same as above
HGB
Same as above
HCT
Same as above
MCV
Same as above
MCH
Same as above
MCHC
Same as above
RDW-SD or RDW-CV
Same as above
Select the output parameter (RDW-SD/RDW-CV)
in the RDW setting of Host output settings.
PLT
Same as above
PDW
Same as above
MPV
Same as above
P-LCR
Same as above
W-SMV
Same as above
W-LMV
Same as above
ETX
(03H)
Total 99
Position of decimal point

The decimal point is not output, numerical data must be obtained by adding a decimal point specified for
parameters by the host computer. This position of the decimal point complies with the national system of
units.
The output sequence is as shown from top to bottom in the above table. Data is sent, without zero
padding, from the most significant digit.
Revised March 2006
This format is fixed at zero (0).
15-30
Sample ID No.
Although the sample ID No. is a numeral with 15 digits, this format outputs 12 digits, deleting the upper
3 digits. - (2DH) may be inserted in the numeral field depending on usage. In this case, - is
included in the 12 digits.
Particle distribution analysis information
The particle distribution analysis information is composed of the following 5 elements.
1)
Parameter
No. of characters
WBC histogram information
WBC histogram flag
RBC histogram information
RBC histogram flag
PLT histogram information
PLT histogram flag
Histogram information
Histogram information contains the following information.
2)
Code
Explanation
Normal histogram
Abnormal histogram
Manually discriminated
Histogram flag
The histogram flags are composed of the following 10 types. Each type corresponds to a
flagging character for the LCD screen and printer.
The histogram flag is 0 for a normal histogram and is 1 - 2 for a histogram error.
Relationship of histogram flags to flagging characters
Revised March 2006
Histogram flag

at HC
Remarks
WBC
RBC
PLT
Normal
Normal
Normal
WL
RL
PL
High frequency at
L discriminator (WBC/RBC/PLT)
WU
RL
PU
High frequency at
H discriminator (WBC/RBC/PLT)
DW
DW
Cannot be analyzed because there is

not an intersection point at the
frequency level of 20% for DW
calculation. (RBC/PLT)
Normal (WBC/RBC/PLT)
15-31
Histogram flag

at HC
WBC
RBC
PLT
MP
MP
Remarks
Multiple peaks (RBC/PLT)
T1
determined. (WBC)
T2
determined. (WBC)
F1, F2
T1 relative frequency exceeded

specified value. (WBC)
F2, F3
Reliability of the data is low due to

high frequency level at T1 or T2
discriminator. (WBC)

Indicates the output status (RDW-CV or RDW-SD).
C :RDW-CV
S :RDW-SD
Numerical data
Numerical data is composed of the following.
The data is 4 significant digits, with the decimal point excluded. It is output without zero-suppression.
Upper digit
Flag
Revised March 2006
Data
Lower digit
15-32
Flag details
Code
Explanation
Normal
Judgment on reference limits: +
Judgment on reference limits: -
Out of linearity limits
Low reliability
Data masking
In the case of data overflow, the data is changed to the following.

*0003
In the case of an analysis error, or analysis parameters with no data, the data is changed to the
following.
*0000
In the Cat mode, the data of PDW, MPV and P-LCR are output as 5 spaces (20H).
In the Cattle or Horse mode, the data of EO% and EO# are output as 5 spaces (20H).
ASTM format
For details on ASTM format, see Section 15.7 ASTM communication specifications.
15.7 ASTM communication specifications

These specifications apply to the mode which conforms to ASTM*1 standards for communication
between pocH-100iV Diff and the host computer.
*1
ASTM (American Society for Testing and Materials)

One of the worlds largest volunteer non-profit organizations, created in 1898 for the purpose of
creating standard regulations for materials, products, and system services.
pocH-100iV Diff supports the following two modes to output data in the ASTM format.
(1) ASTM E1381-02 conforming mode
pocH supports ASTM E1381-02 conforming modes for both serial (RS-232C) and LAN (Ethernet)
connections.
The presentation layer conforms to ASTM E1394-97 and the other layers conform to ASTM E138102.
Revised August 2006
(2) ASTM E1381 non-conforming mode

pocH supports ASTM E1381 non-conforming mode for LAN (Ethernet) connections.
The presentation layer conforms to ASTM E1394-97 and the other layers conform to IEEE 802.3.
Note that it operates in the ASTM E1381 non-conforming mode when [ASTM Rev.] of the host
setting item is set to "1381-95" in the case of LAN (Ethernet) connection.
This mode conforms to the following standards.
ASTM E1381-02
Standard Specification for Low-Level Protocol to Transfer Messages Between
Clinical Laboratory Instruments and Computer Systems
15-33
ASTM E1394-97
Standard Specification for Transferring Information Between Clinical
Instruments and Computer Systems
Communication specifications
The communication specifications are based on a layer protocol.
1. Physical layer
Specifies the sending and receiving of signals between the instrument and host computer through
physical and electrical connections.
See the subsequent section, Physical layer (hardware).
2. Data link layer
Specifies the sending and receiving of data by link connections and for each frame between the
instrument and host computer.
See the subsequent section, Data link layer (transmission protocol)
3. Presentation layer
Specifies the messages that are sent and received by the instrument and host computer.
See the subsequent section, Presentation layer (pocH-100iV Diff communication).
Serial connection mode LAN connection mode
Presentation Layer
ASTM E1394-97
ASTM E1381-02
TCP/IP
RS-232C
Ethernet
Data Link Layer and Physical Layer
ASTM E1381-02 conforming mode

LAN connection mode
ASTM E1394-97
TCP/IP
Ethernet
Presentation Layer
Data Link Layer and Physical Layer
Physical layer (hardware)

1. Physical layer for serial connection
Physical layer of serial (RS-232C) connection for the ASTM E1381-02 conforming mode is described
in this section.
15-34
Revised August 2006
ASTM E1381 non-conforming mode
(1) Connector
A 25-pin D-SUB male connector is standard for ASTM, however pocH-100iV Diff uses a 9-pin DSUB male connector for input/output on the right side of the main unit.
Pin No.
Signal name
Signal direction
NC
Receive Data
RxD
Transmit Data
TxD
Data Terminal Ready
DTR
Signal Ground
SG
Data Set Ready
DSR
Request To Send
RTS
Clear To Send
CTS
NC
The control signal is not used with ASTM. In addition, do not connect any unused pins.
(2) Signal identification level
Level
Data signal
Control signal
+3 V or more
Logic 0, Start bit
ON
-3 V or less
Logic 1, Stop bit
OFF
(3) Connection cable

The analyzer side uses a 9-pin D-SUB female connector, with the following wire connections.
Analyzer side
Host computer side
DB-9
DB-9
DB-25
TxD
RxD
RxD
SG
SG
RTS
RTS
CTS
CTS
DTR
20
DTR
DSR
DSR
NC
NC
1
9
Revised August 2006
TxD
15-35
(4) Interface conditions
Setting
Setting types
Baud rate
1200 / 2400 / 4800 / 9600 / 19200 bps
Data length
7 bits / 8 bits
Stop bit
1 bit / 2 bits
Parity
None / Even / Odd
Establishing the settings underlined allows conformance with ASTM standards.

Note: However, 7-bit data length, even or odd parity, and 2 stop bits are recognized by ASTM for use
with special applications.
(5) Reference standard (ASTM E1381-02)
Except for the shape of the connector, the physical layer conforms to the standards of 5. Physical
Layer of ASTM E1381-02.
The shape of the connector to be used is a 9-pin D-SUB male connector.
(A 25-pin male connector is used in ASTM.)
2. Physical layer for LAN connection
Physical layer of LAN (Ethernet) connection is described in this section.
(1) Connector and Cable
LAN cable (CAT5 cable) is used to connect the analyzer and the host computer.
RJ-45F LAN connector is provided on the right side of the pocH-100iV Diff.
(2) Speed
The data transmission rate for instrument shall conform to IEEE802.3 and operate at least 10 Mbps.
(3) Establishment of a TCP connection
Before the data exchanging, Establishment of a TCP connection is performed.
Under the establishing procedure, pocH-100iV Diff operates as the client, and the host computer as the
server. pocH-100iV Diff requests a connection for the socket ( IP address and the port ) offered by the
host computer.
(4) Reference standard
When [ASTM Rev.] of the host setting item is set to "1381-02", the physical layer conforms to the
standards of "7. Pysical Layer for TCP/IP Data Exchange".
Revised August 2006
In the other cases, the physical layer conforms to IEEE 802.3.
15-36
Data link layer (transmission protocol)
The data layer uses a character-oriented protocol for transfer of messages between systems, according to
the standards of 6. Data Link Layer of ASTM E1381-95.
Following is an outline of the communication control procedure. For details, see ASTM E1381-95.
1. Communications status
The data link layer is composed for the following two communications statuses.
Neutral status
Linked status
Transition to each of these statuses is accomplished through the following 3 phases.

1)
Establishment phase
Establishes a communications line, and determines the direction of data transfer. In this way,
the sender and receiver are identified, and the change is made from neutral status to linked
status.
2)
Transfer phase
The sender transmits messages to the receiver, until all messages have been transferred.
3)
Termination phase
Releases the communications line. Changes both the sender and receiver from linked status to
neutral status.
Establishment phase
Neutral status
Linked status
Transfer phase
Termination phase
2. Establishment phase
1)
Sender sends [ENQ] to receiver. The receiver sends one of the following responses.
Returns [ACK] if communication is possible.
Returns [NAK] if communication is not possible.
If the response from the receiver is [NAK], the sender waits 10 seconds before sending [ENQ]
again.
Max. 15 sec
Max. 15 sec
Sender
Revised August 2006
Receiver
Sender
E
N
Q
A
C
K
Receiver
E
N
Q
10 sec
or more
E
N
Q
N
A
K
15-37
2)
If both sides send [ENQ], the host computer must yield authority to the analyzer.
Analyzer sends [ENQ] again after 1 second.
The host computer must wait 20 seconds before sending [ENQ] again.
Max. 1 sec
Analyzer
E
N
Q
Host computer
E
N
Q
E
N
Q
E
N
Q
Analyzer
Host
computer
E
N
Q
20 sec or more
E
N
Q
3. Transfer phase
During the transfer phase, the sender sends messages to the receiver. The transfer phase continues
until all messages have been sent.
1)
Messages are transferred in multiple frames. Each frame contains a maximum of 247
characters (including frame overhead). If the message is longer than 240 characters, it is
divided into 2 or more frames.
2)
Multiple messages cannot be included in a single text frame.
3)
If the message is 240 characters or less, it is transferred using a text frame with the following
structure.
[STX][F#][Text][ETX][CHK1][CHK2][CR][LF]
If the text is longer than 240 characters, it is divided into 2 or more frames. The intermediate
frame text termination code is [ETB], and the final frame text termination code is [ETX], as
shown below.
[STX][F#][Text][ETB][CHK1][CHK2][CR][LF]
[STX][F#][Text][ETB][CHK1][CHK2][CR][LF]
[STX][F#][Text][ETX][CHK1][CHK2][CR][LF]
15-38
Explanation
[STX]
Start of frame
[F#]
Frame number.
One of the numbers 0 - 7 is used, starting with 1 and repeating 2, 3, 4, 5, 6, 7,
0. In case of retransmission, the same frame number is sent.
[Text]
ASTM E1394-97 records are used. (See the subsequent section, Presentation
layer (pocH-100iV communication).)
For this reason, the codes below are not used.
0x00-0x06, 0x08, 0x0A, 0x0E-0x1F, 0x7F, 0xFF
[ETB]
Control code indicating end of text (for intermediate frame)
[ETX]
Control code indicating end of text (for final frame)
Revised August 2006
Code
Code
Explanation
[CHK1][CHK2]
Expressed by characters 0 - 9 and A - F. Characters beginning from

the character after [STX] and until [ETB] or [ETX] (including [ETB] or
[ETX]) are added in binary. The 2-digit numbers, which represent the least
significant 8 bits in hexadecimal code, are converted to ASCII characters 0
- 9 and A - F. The most significant digit is stored in CHK1 and the
least significant digit in CHK2.
[CR][LF]
Control code indicating end of frame
4)
After the sender sends the frame, sender waits until there is a response from the receiver.
Sender does not send the next frame if a response is not received.
5)
If the receiver has successfully received the frame, and is prepared to receive the next frame,
receiver responds with [ACK]. After the sender receives [ACK], sender advances a frame
number increment and sends either a new frame or the termination frame.
6)
If the receiver fails to receive the frame and is prepared to receive the same frame again,
receiver responds with [NAK]. After the sender receives [NAK], sender sends the most recent
frame again, using the same frame number. If a total of 6 attempts to send the frame fail, sender
transitions to the termination phase and must end sending of the message.
Max. 15 sec
Max. 15 sec
Sixth attempt
First attempt
Sender
...
Text frame #1
N
A
K
Receiver
E
O
T
Text frame #1
N
A
K
...
7)
The analyzer processes the response of [EOT] from the host computer as [ACK].
8)
During the transfer phase, a 15-second timer is set when the final character of the frame is sent
by the sender. Time out results if there is no response within 15 seconds. When the sender
times out, sender transitions to the termination phase. A 30-second timer is set when the
receiver first enters transfer status, and when receiver sends a response ([ACK], [NAK]) for
each frame. Time out results if there is no frame transmission or [EOT] response from the
sender within 30 seconds. When the receiver detects time out, receiver discards the incomplete
message that was being received, and transitions to the termination phase.
Max. 15 sec
Max. 30 sec
Max. 15 sec
Sixth attempt
First attempt
E
O
T
Revised August 2006
Sender
Text frame #1
Receiver
...
Text frame #2
A
C
K
E
O
T
...
15-39
4. Termination phase
During the termination phase, the status returns to neutral.
The sender sends [EOT] to inform the receiver that the message transmission has been completed.
When the sender sends [EOT], sender transitions to neutral status. When the receiver receives [EOT],
receiver transitions to neutral status.
5. Time out
The timer is used to detect a failure to coordinate between the sender and receiver. The timer is used
as a mean of recovery for communications line and communication destination device failures.
1)
During the establishment phase, the timer is set when the sender sends [ENQ]. Time out results
if a response of [ACK], [NAK], or [ENQ] is not received within 15 seconds. After time out, the
sender transitions to the termination phase.
2)
During the transfer phase, the timer is set when the final character of a frame is sent by the
sender. Time out results if no response is received within 15 seconds. After time out, the sender
transitions to the termination phase, and ends message transmission.
Presentation layer (pocH-100iV Diff communication)

Frame transfer sequence
(1) Output of analysis results
pocH-100iV Diff outputs analysis results as shown below.
Analyzer
T1
T2
E
N
Q
T1
T2
T2
T1
T2
Order (O)
Patient (P)
Header (H)
A
C
K
T1
A
C
K
A
C
K
A
C
K
Host computer
T1
T2
T1
T2
A
C
K
A
C
K
Header (H): Header record*1 frame

Patient (P):
Order (O): Order
information
record*1
record*1
T2
T1
T2
E
O
T
Terminate (L)
A
C
K
A
C
K
T1: Max. 15 seconds

frame
frame
T2: Max. 30 seconds

*1: See the subsequent section, Record details.
Result (R): Result record*1 frame

Terminate (L): Message-end code
T1
Result n (R)
A
C
K
Patient*2
T2
Result 2 (R)
Result 1 (R)
T1
*2: Patient means animal.

record*1
frame
Note: This figure is shown so that the data link layer conforms to the ASTM E1381-02 specifications.
In case of ASTM E1381 non-conforming mode, no treatment is performed when the ENQ, ACK and
EOT codes are received.
15-40
Revised August 2006
T2
Messages, records, and fields
1. Messages
In the presentation layer, all data is transmitted using messages. Messages are composed of record
arrays that start with message header record (H) and end with message termination record (L).
2. Records
A record is a series of text, beginning with an ASCII alphabet character referred to as the identifier,
and ending with [CR].
Record type
Record identifier
Level
Contents
Header
Contains information concerning the

message sender and receiver.
Patient information

animal.
Inquiry
Not used
Analysis order

analysis order.
Analysis results

analysis results.
Comments
1-4
Not used
Manufacturer information
1-4
Not used
Scientific information
N/A
Not used
Message-end code
Indicates the end of the message.
Smaller level numbers indicate higher levels.
A high-level record contains information that is common to all lower-level records.
All levels other than 0 must be located after higher levels. However, manufacturer information
(not used) and comment records can be inserted at any level. They are considered to be one level
lower than the preceding record.
3. Fields
Records are further divided by field delimiter into multiple fields.
A field is identified by its position within a record, and has a variable length.
The followings are used as delimiter.
Delimiter type
Code
Contents
Divides the fields within a record.
Repetition delimiter
Used to repeat the same field when the same type of component
exists multiple times in 1 field.
Component delimiter
Further divides 1 field into a number of sub-fields.
Escape delimiter
&
Used to embed a special character into the data.

(| &F&, \ &R&, ^ &S&, & &E&,
Hexadecimal &Xxxxx&)
Revised August 2006
Field delimiter
15-41
Record 1
Record 2
Field 1
Field 2
Record n
Field n
[CR]
Delimiter | or \
Record identifier
Component 1
Component 2
Component n
Delimiter ^
Record details
1. Header record
Example of transmission:
pocH100iV Diff Host computer
H|\^&|||pocH-100iV Diff^00-00^^^^Sysmex pocHVD 01^12345678||||||||E1394-97[CR]
Host computer pocH-100iV Diff
H|\^&|||||||||||E1394-97[CR]
15-42
Field name
Analyzer
Host computer
Host computer
Analyzer
7.1.1
Record type
7.1.2
Delimiter definition
|\^&
|\^&
7.1.3
Message control ID
Not used
Not used
7.1.4
Access password
Not used
Not used
7.1.5
Sender name or ID
Instrument name^
Software version^^^^
User instrument No.
^PS code
Not used
7.1.6
Sender address
Not used
Not used
7.1.7
Reserved
Not used
Not used
7.1.8
Sender telephone No.
Not used
Not used
7.1.9
Sender characteristics
Not used
Not used
7.1.10
Receiver ID
Not used
Not used
7.1.11
Comment
Not used
Not used
7.1.12
Process ID
Not used
Not used
7.1.13
Version No.
E1394-97
E1394-97
7.1.14
Date and time
Not used
Not used
Remarks
pocH-100iV Diff^0000^^^^Sysmex
pocHVD 01
^12345678
Fixed
Revised August 2006
ASTM
field
Detailed explanation of fields:
1)
7.1.2 Delimiter definitions

|\^& is used as a fixed character string. There is no need to use a delimiter between fields
7.1.1 and 7.1.2.
2)
7.1.5 Sender name or ID

The instrument name is fixed to pocH-100iV Diff. The instrument ID is fixed to 00-00 for
each software version. Each time the software version is upgraded, the instrument ID is
updated to 00-01, 00-02, and so on. The user instrument No. is the Serial No. set by
Settings - User information.
2. Patient records
pocH-100iV Diff Host computer
P|1[CR]
Not used.
Revised August 2006
ASTM
field
Field name
Analyzer
Host computer
Host computer
Analyzer
Sequence No.
Not used
8.1.1
Record type
8.1.2
Sequence No.
8.1.3
Patient ID assigned
by attending
physician
Not used
Not used
8.1.4
Patient ID assigned
by laboratory
Not used
Not used
8.1.5
Patient ID
Not used
Not used
8.1.6
Patient name
Not used
Not used
8.1.7
Mothers maiden
name
Not used
Not used
8.1.8
Date of birth
Not used
Not used
8.1.9
Patient sex
Not used
Not used
8.1.10
Patient race
Not used
Not used
8.1.11
Patient address
Not used
Not used
Remarks
Sequence No.
indicating the order
in which this type of
record appears in the
message. This
number begins with
1, and is reset to 1
each time a record at
a higher level appears
in the message.
15-43
15-44
Field name
Analyzer
Host computer
Host computer
Analyzer
8.1.12
Reserved field
Not used
Not used
8.1.13
Patient telephone No.
Not used
Not used
8.1.14
ID of attending
physician
Not used
Not used
8.1.15
Special field 1
Not used
Not used
8.1.16
Special field 2
Not used
Not used
8.1.17
Patient height
Not used
Not used
8.1.18
Patient weight
Not used
Not used
8.1.19
Presumed diagnosis
of patient
Not used
Not used
8.1.20
Drug treatment for

patient
Not used
Not used
8.1.21
Dietary treatment for

patient
Not used
Not used
8.1.22
Attending physician
field 1
Not used
Not used
8.1.23
Attending physician
field 2
Not used
Not used
8.1.24
Dates patient was

admitted and
discharged
Not used
Not used
8.1.25
Condition when
admitted
Not used
Not used
8.1.26
Location
Not used
Not used
8.1.27
Alternative
diagnosis code and
type of classifier
Not used
Not used
8.1.28
Alternative
diagnosis code and
classification
Not used
Not used
8.1.29
Patient religion
Not used
Not used
8.1.30
Marital status
Not used
Not used
8.1.31
Isolation status
Not used
Not used
8.1.32
Language
Not used
Not used
8.1.33
Hospital service
Not used
Not used
8.1.34
Hospital Institution
Not used
Not used
8.1.35
Dosage category
Not used
Not used
Remarks
Revised August 2006
ASTM
field
Detailed explanation of fields:
1)
8.1.2 Sequence No.

The sequence number starts with 1 and indicates the sequence position in which the record
appeared in the message. This number is reset to 1 when a higher-level record appears in the
message.
3. Order record
O|1||^^1234567890ABCDEÂ|^^^^WBC\^^^^RBC\^^^^HGB\^^^^HCT\^^^^MCV
\^^^^MCH\^^^^MCHC\^^^^PLT\^^^^LYM%\^^^ÔTHR%\^^^ÊO%\^^^^
LYM#\^^^ÔTHR#\^^^ÊO#\^^^^RDW-SD\^^^^RDW-CV\^^^^PDW
\^^^^MPV\^^^^P-LCR|||||||N|||||||(1)Dog|||||||F[CR]
Not used.
ASTM
field
9.4.1
9.4.2
9.4.3
9.4.4
Field name
Record type
Sequence No.
Specimen ID
Instrument
specimen ID
Analyzer
Host computer
O
Sequence No.
Host computer
Analyzer
Not used
Not used
Not used
^^Sample ID^
Sample ID
attributes
Not used
Not used
Remarks
Sequence No.
indicating the order in
which this type of
message. This number
begins with 1, and is
reset to 1 each time a
record at a higher level
appears in the
message.
A sample ID less than
15 digits is extended to
15 digits by space
padding or zero
padding in accordance
with the settings of ID
Pad. made by
Settings - Host
output.
Sample ID attributes
are as follows:
M: Manually input
Revised August 2006
A: Automatically
allocated
B: Read by ID reader
15-45
ASTM
field
9.4.5
Field name
Analysis parameter
ID
Analyzer
Host computer
^^^^Parameter
name
Host computer
Analyzer
Not used
Remarks
Parameter names:
WBC, RBC, HGB,
HCT, MCV, MCH,
MCHC, PLT,
LYM%, OTHR%,
EO%,
LYM#, OTHR#, EO#,
RDW-SD,RDW-CV,
PDW,MPV,P-LCR
W-SMV,W-LMV
Repetition delimiter is
used to output multiple
parameters.
Example:
^^^^
Parameter name1\
^^^^
Parameter name2\
^^^^
Parameter name3
* LYM%, OTHR%,
EO%, LYM#,
OTHR# and EO# are
output as W-SCR,
W-MCR, W-LCR,
W-SCC, W-MCC
and W-LCC
respectively, When
the analysis was
performed in the
OTHER1 OTHER13 mode.
9.4.6
9.4.7
9.4.8
9.4.9
9.4.10
15-46
Priority sequence
Date and time of
order
Date and time of
collection
Time specimen
collection end
Collection volume
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Revised August 2006
*W-SMV and
WLMV in the
analysis data are not
output.
*PDW, MPV and PLCR in the Cat
mode are not output.
*EO% and EO# in
the Cattle or
Horse mode are not
output.
ASTM
field
9.4.11
9.4.12
Analyzer
Host computer
Not used
N, Q
Host computer
Analyzer
Not used
Not used
Danger code
Related clinical
information
Date/time specimen
was received
Specimen
descriptor
Ordering physician
Physicians
telephone No.
User field 1
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Species
Not used
User field 2
Laboratory field 1
Laboratory field 2
Date/time results
were reported or
last modified
Fee for instrument
use
Analyzer ID
Report type
Reserved
Location or ward of
specimen collected
Hospital infection
flag
Specimen service
Specimen
institution
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
F
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Not used
Field name
Collector ID
Action code
Remarks
N: Normal sample
code
Q: QC data
9.4.13
9.4.14
9.4.15
9.4.16
9.4.17
9.4.18
9.4.19
9.4.20
9.4.21
9.4.22
9.4.23
9.4.24
9.4.25
9.4.26
9.4.27
9.4.28
9.4.29
9.4.30
9.4.31
example: (1)Dog,
(5)OTHERRESEARCH-
F: fixed
4. Result record
R|1|^^^^WBC^1|7.8|10*3/L||N||||||20011221163530 [CR]
R|2|^^^^RBC^1|3.50|10*6/L||L||||||20011221163530 [CR]
Revised August 2006
R|3|^^^^HGB^1|***.*|g/dL||A||||||20011221163530 [CR]
...
R|19|^^^^P-LCR^1|50.0|%||H||||||20011221163530 [CR]
Not used.
15-47
Caution!
The analysis data of the OTHER1 - OTHER13 mode is for research.
ASTM
field
Note:
PDW, MPV and P-LCR in the Cat mode are not output.
EO% and EO# in the Cattle mode are not output.

Field name
10.1.1
Record type
10.1.2
Sequence No.
10.1.3
Analysis parameter
ID
Analyzer
Host computer
Host computer
Analyzer
Not used
Sequence No.
Not used
Sequence No.
indicating the order
in which this type of
message. This
number begins with
1, and is reset to 1
each time a record at
a higher level appears
in the message.
^^^^Parameter
name^Dilution ratio
Not used
Parameter name:
See the analysis
parameter ID (ASTM
field) of analysis
order record.
Remarks
10.1.4
Data value
Data value
Not used
Data values:
These are output after
a decimal point is
added in the correct
position, according to
the units which are
set.
For data which is
masked for display,
the mask codes are
output in order of
priority, in the same
way as they appear
on the screen.
Example: +++.+
15-48
Revised August 2006
Dilution ratio:
1, WB mode
(fixed)
ASTM
field
10.1.5
Field name
Units
Analyzer
Host computer
Host computer
Analyzer
Remarks
Units
Not used
[Units]
These are output in
the format
determined by the
units setting.
Exponents are
expressed using *.
Example:
103/L 10*3/L
10.1.6
Reference ranges
10.1.7
Analysis result error

flag
Not used
Not used
L, H, >, N, A, W
Not used
A:
Data masked for
display
>:
Data with flag !
W:
Data with flag *
H:
Data with flag +
L:
Data with flag -
N:
Any (normal) data
other than the above
The order of priority
is, from highest to
lowest: A, >,
W, H, L, N.
Type of error
analysis
Not used
Not used
10.1.9
Analysis result status
Not used
Not used
10.1.10
Date of change to
reference value/units
Not used
Not used
10.1.11
Operator ID
Not used
Not used
10.1.12
Date and time

analysis started.
Not used
Not used
Revised August 2006
10.1.8
15-49
ASTM
field
10.1.13
Field name
Date and time
analysis completed.
Analyzer
Host computer
Host computer
Analyzer
Remarks
YYYYMMDD
hhmmss
Not used
The year-month-dayhour-time-second
sequence for the date
is fixed, regardless of
the date format set
for the system
settings.
YYYY:
Year (4 digits*1)
MM:
Month (2 digits*1)
DD:
Day (2 digits*1)
hh:
Hour (2 digits*1)
mm:
Time (2 digits*1)
ss:
Second (2 digits*1)
*1: With zero
padding.
10.1.14
Analyzer ID
Not used
Not used
Analyzer
Host computer
Host computer
Analyzer
5. Message-end code record

L|1|N[CR]
L|1|N[CR]
ASTM
field
Field name
Remarks
Record type
13.1.2
Sequence No.
Always 1
13.1.3
End code
N: Normal
termination
Revised August 2006
13.1.1
15-50
15.8 System of units

Type1 (Japan)
Revised August 2006
Item
Decimal
point
Unit
WBC
####
102 /L
RBC
####
104 /L
Type2 (For General Export)

Decimal
point
###.#
##.##
Unit
103 /L
106 /L
Type3 (Canada SI)

Decimal
point
###.#
##.##
109 /L
1012 /L
HGB
###.#
g/dL
###.#
g/dL
HCT
###.#
###.#
MCV
###.#
fL
###.#
fL
###.#
fL
MCH
###.#
pg
###.#
pg
###.#
pg
MCHC
###.#
g/dL
###.#
g /dL
####
g /L
PLT
###.#
104 /L
####
103 /L
####
109 /L
LYM%
###.#
###.#
#.###
OTHR%
###.#
###.#
#.###
EO%
###.#
###.#
#.###
LYM#
####
102 /L
###.#
103 /L
###.#
109 /L
OTHR#
####
102 /L
###.#
103 /L
###.#
109 /L
EO#
####
102 /L
###.#
103 /L
###.#
109 /L
W-SCR
###.#
###.#
#.###
W-MCR
###.#
###.#
#.###
W-LCR
###.#
###.#
#.###
W-SCC
####
102 /L
###.#
103 /L
###.#
109 /L
W-MCC
####
102 /L
###.#
103 /L
###.#
109 /L
W-LCC
####
102 /L
###.#
103 /L
###.#
109 /L
RDW-SD
###.#
fL
###.#
fL
###.#
fL
RDW-CV
###.#
###.#
MPV
###.#
fL
###.#
fL
###.#
fL
PDW
###.#
fL
###.#
fL
###.#
fL
P-LCR
###.#
###.#
####
Unit
#.###
g/L
L/L
#.###
#.###
15-51
Item
WBC
RBC
HGB
HCT
MCV
Decimal
point
###.#
##.##
###.#
#.###
###.#
Unit
109 /L
1012 /L
mmol /L
L/L
Type5 (Standard SI)

Decimal
point
###.#
##.##
####
Unit
109 /L
1012 /L
g/L
Type6 (Hong Kong SI)

Decimal
point
###.#
##.##
###.#
#.###
Unit
109 /L
1012 /L
g/dL
#.###
fL
###.#
fL
###.#
fL
MCH
####
amol
###.#
pg
###.#
pg
MCHC
###.#
mmol /L
####
g/L
###.#
g/dL
PLT
####
109 /L
####
109 /L
####
109 /L
LYM%
#.###
#.###
###.#
OTHR%
#.###
#.###
###.#
EO%
#.###
#.###
###.#
LYM#
###.#
109 /L
###.#
109 /L
###.#
109 /L
OTHR#
###.#
109 /L
###.#
109 /L
###.#
109 /L
EO#
###.#
109 /L
###.#
109 /L
###.#
109 /L
W-SCR
#.###
#.###
###.#
W-MCR
#.###
#.###
###.#
W-LCR
#.###
#.###
###.#
W-SCC
###.#
109 /L
###.#
109 /L
###.#
109 /L
W-MCC
###.#
109 /L
###.#
109 /L
###.#
109 /L
W-LCC
###.#
109 /L
###.#
109 /L
###.#
109 /L
RDW-SD
###.#
fL
###.#
fL
###.#
fL
###.#
RDW-CV
#.###
#.###
MPV
###.#
fL
###.#
fL
###.#
fL
PDW
###.#
fL
###.#
fL
###.#
fL
###.#
P-LCR
15-52
#.###
#.###
Revised August 2006
Type4 (Dutch SI)
15.9 Program card replacement procedure

The program card contains all programs for this instrument. Under
normal conditions, it will not be removed.
However, if the card is damaged, it must be replaced.
Following is an explanation of the procedures for removing, inserting,
and replacing the program card.
Removing the program card
1. Check that the main switch is OFF.
2. Press the button (once) at the top of the card slot on the right side of
the instrument.
The button will pop out.
3. Press the button again. Press it in until a "click" sound is heard.
Revised August 2006
The program card will be ejected.
4. Check that the button is button is "in", then remove the card.
15-53
Separating the adapter
1. When the program card is removed from the card slot, it is attached
to an adapter.
adapter
Pull the adapter to remove it.
Installing the program card

1. Insert the new program card into the adapter.
2. Insert into the card slot until a solid click is felt.
Checking the program
1. Turn the main switch ON.
2. Check that operation is normal, and that the Main screen appears.
15.10 Checking the program version

Ready
Menu
Main

ID
1
Set a mixed
sample, and
Press [RUN].
Species (1)Dog
RUN
select
Result
Not Ready
Menu
Top

Str.Data
Chg.Reag
Calib.
Settings
15-54
Shutdown
Maint.
Revised August 2006
QC
Not Ready
Top
Maint.
Auto Rinse
Status Display
Clog Removal
Calibration LCD
Drain TD Chamber
Print Error Log
Clean Transducer
Setting Seq.
Clean W. Chamber
Drain Reagent
Ready
The status display screen will appear. Check the version of the
program.
Top
Maint.
Vacuum
HGB Convert
Sensor
SV
3. Press the [Status Display] button.
0. 0130 MPa
2012 (255)
1 2 3 4 5 6 7 8
1 2 3 4 5 6 7 8 9 0
1 2 3 4 5 6 7 8 9 0
1 2 3 4 5 6 7 8 9 0
1 2 3 4 5 6 7 8 9 0
123456
123456 (17/05/2000)
123456 (17/05/2000)
00-00 (BIOS: 00-00)
Counter
Waste C.
Detector
Version
Run
15.11 Functional description

Detection principle
This section describes blood cell count detection principle and analysis
method used in this instrument.
Detection Principle:DC detection method, Hydrodynamic Focusing

DC detection method and non-cyanide
hemoglobin analysis method.
DC detection method
DC
DC Supply
Transducer Chamber
External Electrode +
Resistance
Blood Cell Suspension
Revised August 2006
Aperture Internal Electrode -
Blood sample is aspirated, measured to a predetermined volume, diluted

at the specified ratio, then fed into each transducer. The transducer
chamber has a minute hole called the aperture. On both side of the
aperture, there are the electrodes between which flows direct current.
Blood cells suspended in the diluted sample pass through the aperture,
causing direct current resistance to change between the electrodes. As
direct current resistance changes, the blood cell volume is detected as
electric pulses.
Blood cell count is calculated by counting the pulses, and a histogram of
blood cell volume is plotted by determining the pulse heights. Also,
analyzing a histogram makes it possible to obtain various analysis data.
15-55
Hydrodynamic focusing DC detection method
Inside the detector, the sample nozzle is positioned in front of the
aperture and in line with the center. After diluted sample is forced from
the sample nozzle into the conical chamber, it is surrounded by front
sheath reagent and passes through the aperture center.
Aperture
Front sheath reagent
Sample nozzle
The Hydro Dynamic Focusing DC detection method improves blood

count accuracy and reproducibility. And because the blood cells pass
through the aperture in a line, it also prevents the generation of abnormal
blood cell pulses.
Non-Cyanide Hemoglobin analysis method

To analyze hemoglobin by automated methods, the Cyanmethemoglobin
method or Oxyhemoglobin method have so far been the main stream.
Cyanmethemoglobin method was recommended as the international
standard method in 1966 by ICSH (International Committee for
Standardization in Haematology). This method, however, is so low in
hemoglobin conversion rate that it cannot be said an appropriate method
in the automated process in which multi-sample processing is the precondition. In addition, this method uses the reagent of cyanide
compound which is a poisonous substance and requires waste
processing; thus, it can hardly be called an environmentally favorable
method.
At present, this method cannot be said suitable for a fully-automated
instrument which is required to handle a large amount of waste.
The Oxyhemoglobin method, on the other hand, is faster in hemoglobin
conversion rate; in fact, blood hemoglobin is converted instantaneously
into oxyhemoglobin. Also, it does not contain poisonous substance as
cyanmethemoglobin method, making the method suitable for
automation. This method, however, is unable to convert methemoglobin
into oxyhemoglobin. Consequently, when a great amount of
methemoglobin is contained as in control blood, lower-than-real values
result, although usual human blood poses no problems.
Non-cyanide hemoglobin analysis method utilizes the advantages of both
of the above methods. Non-cyanide hemoglobin analysis method rapidly
converts blood hemoglobin as the Oxyhemoglobin method and contains
no poisonous substance, making it suitable for automated method.
Revised August 2006
Being capable of analyzing methemoglobin, this method can accurately

analyze control blood, etc. which contain methemoglobin.
15-56
Measuring unit hydraulic system block diagram
<Whole Blood Mode>
0.59 mL
WBC/HGB Lyse
0.72 mL
1:130 Diluted Sample (461 L)
WBC/HGB
TD Chamber
(Approx. 1:500)
Diluent
1.9 mL
RBC/PLT
Sample Chamber
(Approx. 1:1360)
1.935 mL
Whole Blood Sample (15 L)
Aspirating Pipette
1:130 Diluted Sample

(200 L)
Mixing Chamber
(Approx. 1:130)
WBC Counting Approx. 0.33 mL

RBC Counting Approx. 14.7 L
Whole Blood Sample (15 L)
Calculation of RBC constant

RBC constant (mean RBC volume, mean RBC hemoglobin, mean RBC hemoglobin concentration) is
calculated from RBC, HGB, and HCT.
1. Mean RBC Volume (MCV)
Calculation is made from RBC and HCT by the formula below:
MCV (fL) =
HCT (%)
6
RBC (10 /L)
10
2. Mean RBC Hemoglobin (MCH)

Calculation is made from RBC and HGB by the formula below:
MCH (pg) =
HGB (g/dL)
6
RBC (10 /L)
10
3. Mean RBC Hemoglobin Concentration (MCHC)

Calculation is made from HCT and HGB by the formula below:
HGB (g/dL)
HCT (%)
100
Revised August 2006
MCHC (g/dL) =
15-57
Electric system
The microprocessor in the main unit controls the hydraulic system's solenoid valves and syringe, thus
regulating the flow of samples, reagents, and waste in the hydraulic system.
Electric signals received from various transducers go through the analogue circuit for electrical
waveform-processing, and to the microcomputer. The microcomputer converts the analogue signals into
digital signals for the calculation.
The WBC, RBC, and PLT cell signals are sent to the respective waveform-processing circuits in the
analogue circuit, where the noise in signals is eliminated to acquire the required cell signals only. The
microcomputer converts the A/D-converted cell signals into particle distribution data, and outputs them
to the built-in printer or to the host computer.
Revised August 2006
To calculate HGB, absorbance of only the diluent (background) is deducted from samples' absorbance.
The beam that has passed through the fluid is detected by the photo diode. And the signals is
photoelectrically converted, A/D converted, and then sent to the HGB counting circuit for the calculation
of the absorbance.
15-58
Power Supply Unit
HGB
Amplification
Circuit
HGB
Detector
Temperature
Monitoring
Circuit
Pressure
Monitoring
Circuit
WBC,
RBC, PLT
Detection Circuit
Analogue Circuit
WBC,
RBC, PLT
Transducer
Transducer
Main Unit
Revised August 2006
Power Supply
Circuit
HGB
Conversion
Circuit
WBC, PLT
Amplification
Waveform
Processing
Circuit
RBC
Waveform
Processing
Circuit
Driver
Solenoid
Motor
A-D
Conversion
Circuit
PDA Unit
PDA Unit
RBC A-D
Conversion
Circuit
PLT, WBC A-D
Conversion
Circuit
InputOutput
Interface
Data
Logger
HGB Counting Circuit
Microcomputer Circuit
Printer
Interface
Interface
LCD
Interface
Serial Interface
Processor
Memory
Built-in
Printer
Operation
Unit
Display Unit
HC Output
Built-in Printer
Touch Panel
LCD Display
(Computer)
Electric diagram
15-59
Bus
Revised August 2006
15-60
Warranty
16. Warranty
All Sysmex instruments are warranted against defective material or
workmanship for a period of one year, commencing on the installation
date at the customer's premises.
This warranty does not cover any defect, malfunction or damage due to:
Accident, neglect or wilful mistreatment of the product;
Failure to use, operate, service or maintain the product in accordance

with the applicable Sysmex Instructions for Use;
Failure to use the appropriate reagents and consumables specified for

the product.
July 2005
16-1
July 2005
Warranty
16-2
Glossary
17. Glossary
CBC8-Parameter; Complete Blood Count
WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT
Resistance measuring method
This method for counting blood cells makes use of the fact that the blood
cells to be counted act as insulator in an electrolytic solution. The device
for counting the cells consists of an electric static field, limited by an
aperture. When blood cells pass through the aperture, an electrolyte
volume equivalent to that of the blood cell is displace, causing changes
in resistance, which are detected as measurable variable.
Photometric measuring method
An optical measuring method is used for the determination of
hemoglobin concentration. From the extinction, measured at the
maximum hemoglobin absorption of blood sample diluted and lysed
under standardized conditions, the HGB concentration is determined.
Discriminator
With discriminators or thresholds, the different populations of a multiple
counting result are demarcated against each other (discriminated).
Histogram
The graphical representation of quantified data in a line or column chart,
where the frequency of each measured value is represented by the size
(or height) of a rectangular over the corresponding data class of the
abscissa. In hematology, a histogram is the volume frequency
distribution of blood cells, from which statements on quantity and
quality of the sample can be derived.
Histogram parameter
Revised March 2006
LYM%, OTHR%, EO%, LYM#, OTHR#, EO#, RDW-SD, RDW-CV,

PDW, MPV, P-LCR
17-1
Revised March 2006
Glossary
17-2
Sysmex pocH-100i
Appendix
18. Appendix
Maintenance Record pocH-100iV Diff
Year:
Month:
Daily
Maintenance item
Day
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
Clean TD chambers
and hydraulic system
(Shutdown)
Signature
Maintenance item
Day
Clean TD chambers
(Shutdown)
Signature
Maintenance item
Day
Clean TD chambers
(Shutdown)
Signature
Every 2 weeks
Maintenance item
Week
Clean transducer
July 2005
Signature
18-1
Appendix
Every 3 months
Year:
Maintenance item
Month:
Month/Day & Signature
Clean waste chamber

As-needed maintenance
Maintenance item
Perform Auto Rinse
Dispose of waste fluid
Clean the sample tube adapter
Remove a clog from transducer aperture
Trap chamber checking and draining
Clean aperture of transducer chamber
Drain reagent
Calibrate LCD
Reagent Replenishing Record
pocH-pack D
Month/Day
Expiration
date
Signature
Lot ID
Month/Day
Expiration
date
Signature
July 2005
Lot ID
18-2
Appendix
pocH-pack LVD
Lot ID
Month/Day
Expiration
date
Signature
Lot ID
Month/Day
Expiration
date
Signature
Replace supplies
Maintenance item
Month/Day &
Signature
Month/Day &
Signature
Month/Day &
Signature
Month/Day &
Signature
Replace fuses
July 2005
Replace printer paper
18-3
Appendix
Optional parts list
Part No.
Part name
Explanation
TOUCH65-PRO
Bar code reader unit
265-9151-5
CAB320-D1N8
Connects the bar code reader to the

instrument.
281-9577-5
SPC-Touch 65/90 Desk Holder
Stand for the bar code reader
265-4728-3
POWER CORD NO.7650
POWER CORD for U.K.
442-3032-1
COLLECTION TUBE
(12-15 MM DIAMETER)
Color: black
July 2005
281-9576-1
18-4
GNU GENERAL PUBLIC LICENSE
19. GNU GENERAL PUBLIC LICENSE

A portion of the software used with this product is subject to the GNU General Public License.
Person wishing to obtain the source code or detailed information concerning the software used with this product
that is subject to the GNU General Public License should contact the Sysmex service representative.
For the software used with this product that is not subject to the GNU General Public License, the source code
may not be obtained, reverse engineered, decompiled, or disassembled.

Version 2, June 1991
Copyright (C) 1989, 1991 Free Software Foundation, Inc.
59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not
allowed.
Preamble
The licenses for most software are designed to take away your freedom to share and change it. By contrast, the
GNU General Public License is intended to guarantee your freedom to share and change free software--to make sure
the software is free for all its users. This General Public License applies to most of the Free Software Foundation's
software and to any other program whose authors commit to using it. (Some other Free Software Foundation software
is covered by the GNU Library General Public License instead.) You can apply it to your programs, too.
When we speak of free software, we are referring to freedom, not price. Our General Public Licenses are designed
to make sure that you have the freedom to distribute copies of free software (and charge for this service if you wish),
that you receive source code or can get it if you want it, that you can change the software or use pieces of it in new
free programs; and that you know you can do these things.
To protect your rights, we need to make restrictions that forbid anyone to deny you these rights or to ask you to surrender the rights.
These restrictions translate to certain responsibilities for you if you distribute copies of the software, or if you modify
it.
For example, if you distribute copies of such a program, whether gratis or for a fee, you must give the recipients all
the rights that you have. You must make sure that they, too, receive or can get the source code. And you must show
them these terms so they know their rights.
We protect your rights with two steps: (1) copyright the software, and (2) offer you this license which gives you
legal permission to copy, distribute and/or modify the software.
Also, for each author's protection and ours, we want to make certain that everyone understands that there is no warranty for this free software. If the software is modified by someone else and passed on, we want its recipients to know
that what they have is not the original, so that any problems introduced by others will not reflect on the original
authors' reputations.
Finally, any free program is threatened constantly by software patents. We wish to avoid the danger that redistributors of a free program will individually obtain patent licenses, in effect making the program proprietary. To prevent
this, we have made it clear that any patent must be licensed for everyone's free use or not licensed at all.
July 2005
The precise terms and conditions for copying, distribution and modification follow.
19-1

TERMS AND CONDITIONS FOR COPYING, DISTRIBUTION AND MODIFICATION
0. This License applies to any program or other work which contains a notice placed by the copyright holder saying
it may be distributed under the terms of this General Public License. The "Program", below, refers to any such program or work, and a "work based on the Program" means either the Program or any derivative work under copyright law: that is to say, a work containing the Program or a portion of it, either verbatim or with modifications and/
or translated into another language. (Hereinafter, translation is included without limitation in the term "modification".) Each licensee is addressed as "you".
Activities other than copying, distribution and modification are not covered by this License; they are outside its
scope. The act of running the Program is not restricted, and the output from the Program is covered only if its contents constitute a work based on the Program (independent of having been made by running the Program).
Whether that is true depends on what the Program does.
1. You may copy and distribute verbatim copies of the Program's source code as you receive it, in any medium, provided that you conspicuously and appropriately publish on each copy an appropriate copyright notice and disclaimer of warranty; keep intact all the notices that refer to this License and to the absence of any warranty; and
give any other recipients of the Program a copy of this License along with the Program.
You may charge a fee for the physical act of transferring a copy, and you may at your option offer warranty protection in exchange for a fee.
2. You may modify your copy or copies of the Program or any portion of it, thus forming a work based on the Program, and copy and distribute such modifications or work under the terms of Section 1 above, provided that you
also meet all of these conditions:
a) You must cause the modified files to carry prominent notices stating that you changed the files and the date of
any change.
b) You must cause any work that you distribute or publish, that in whole or in part contains or is derived from
the Program or any part thereof, to be licensed as a whole at no charge to all third parties under the terms of
this License.
c) If the modified program normally reads commands interactively when run, you must cause it, when started
running for such interactive use in the most ordinary way, to print or display an announcement including an
appropriate copyright notice and a notice that there is no warranty (or else, saying that you provide a warranty) and that users may redistribute the program under these conditions, and telling the user how to view a
copy of this License. (Exception: if the Program itself is interactive but does not normally print such an
announcement, your work based on the Program is not required to print an announcement.)
These requirements apply to the modified work as a whole. If identifiable sections of that work are not derived from
the Program, and can be reasonably considered independent and separate works in themselves, then this License, and
its terms, do not apply to those sections when you distribute them as separate works. But when you distribute the
same sections as part of a whole which is a work based on the Program, the distribution of the whole must be on the
terms of this License, whose permissions for other licensees extend to the entire whole, and thus to each and every
part regardless of who wrote it.
Thus, it is not the intent of this section to claim rights or contest your rights to work written entirely by you; rather,
the intent is to exercise the right to control the distribution of derivative or collective works based on the Program.
July 2005
In addition, mere aggregation of another work not based on the Program with the Program (or with a work based on
the Program) on a volume of a storage or distribution medium does not bring the other work under the scope of this
License.
19-2
3. You may copy and distribute the Program (or a work based on it, under Section 2) in object code or executable
form under the terms of Sections 1 and 2 above provided that you also do one of the following:
a) Accompany it with the complete corresponding machine-readable source code, which must be distributed
under the terms of Sections 1 and 2 above on a medium customarily used for software interchange; or,
b) Accompany it with a written offer, valid for at least three years, to give any third party, for a charge no more
than your cost of physically performing source distribution, a complete machine-readable copy of the corresponding source code, to be distributed under the terms of Sections 1 and 2 above on a medium customarily
used for software interchange; or,
c) Accompany it with the information you received as to the offer to distribute corresponding source code. (This
alternative is allowed only for noncommercial distribution and only if you received the program in object
code or executable form with such an offer, in accord with Subsection b above.)
The source code for a work means the preferred form of the work for making modifications to it. For an executable
work, complete source code means all the source code for all modules it contains, plus any associated interface definition files, plus the scripts used to control compilation and installation of the executable. However, as a special exception, the source code distributed need not include anything that is normally distributed (in either source or binary
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unless that component itself accompanies the executable.
If distribution of executable or object code is made by offering access to copy from a designated place, then offering
equivalent access to copy the source code from the same place counts as distribution of the source code, even though
third parties are not compelled to copy the source along with the object code.
4. You may not copy, modify, sublicense, or distribute the Program except as expressly provided under this License.
Any attempt otherwise to copy, modify, sublicense or distribute the Program is void, and will automatically terminate your rights under this License.
However, parties who have received copies, or rights, from you under this License will not have their licenses terminated so long as such parties remain in full compliance.
5. You are not required to accept this License, since you have not signed it. However, nothing else grants you permission to modify or distribute the Program or its derivative works. These actions are prohibited by law if you do
not accept this License. Therefore, by modifying or distributing the Program (or any work based on the Program),
you indicate your acceptance of this License to do so, and all its terms and conditions for copying, distributing or
modifying the Program or works based on it.
6. Each time you redistribute the Program (or any work based on the Program), the recipient automatically receives
a license from the original licensor to copy, distribute or modify the Program subject to these terms and conditions.
You may not impose any further restrictions on the recipients' exercise of the rights granted herein. You are not
responsible for enforcing compliance by third parties to this License.
7. If, as a consequence of a court judgment or allegation of patent infringement or for any other reason (not limited to
patent issues), conditions are imposed on you (whether by court order, agreement or otherwise) that contradict the
conditions of this License, they do not excuse you from the conditions of this License. If you cannot distribute so
as to satisfy simultaneously your obligations under this License and any other pertinent obligations, then as a consequence you may not distribute the Program at all. For example, if a patent license would not permit royalty-free
redistribution of the Program by all those who receive copies directly or indirectly through you, then the only way
you could satisfy both it and this License would be to refrain entirely from distribution of the Program.
July 2005
If any portion of this section is held invalid or unenforceable under any particular circumstance, the balance of the
section is intended to apply and the section as a whole is intended to apply in other circumstances.
19-3
It is not the purpose of this section to induce you to infringe any patents or other property right claims or to contest
validity of any such claims; this section has the sole purpose of protecting the integrity of the free software distribution system, which is implemented by public license practices. Many people have made generous contributions to the
wide range of software distributed through that system in reliance on consistent application of that system; it is up to
the author/donor to decide if he or she is willing to distribute software through any other system and a licensee cannot
impose that choice.
This section is intended to make thoroughly clear what is believed to be a consequence of the rest of this License.
8. If the distribution and/or use of the Program is restricted in certain countries either by patents or by copyrighted
interfaces, the original copyright holder who places the Program under this License may add an explicit geographical distribution limitation excluding those countries, so that distribution is permitted only in or among countries not
thus excluded. In such case, this License incorporates the limitation as if written in the body of this License.
9. The Free Software Foundation may publish revised and/or new versions of the General Public License from time
to time. Such new versions will be similar in spirit to the present version, but may differ in detail to address new
problems or concerns.
Each version is given a distinguishing version number. If the Program specifies a version number of this License
which applies to it and "any later version", you have the option of following the terms and conditions either of that
version or of any later version published by the Free Software Foundation. If the Program does not specify a version
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10. If you wish to incorporate parts of the Program into other free programs whose distribution conditions are different, write to the author to ask for permission. For software which is copyrighted by the Free Software Foundation,
write to the Free Software Foundation; we sometimes make exceptions for this. Our decision will be guided by the
two goals of preserving the free status of all derivatives of our free software and of promoting the sharing and reuse
of software generally.
NO WARRANTY
11. BECAUSE THE PROGRAM IS LICENSED FREE OF CHARGE, THERE IS NO WARRANTY FOR THE
PROGRAM, TO THE EXTENT PERMITTED BY APPLICABLE LAW. EXCEPT WHENOTHERWISE
STATED IN WRITING THE COPYRIGHT HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM "AS IS" WITHOUT WARRANTY OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS
FOR A PARTICULAR PURPOSE. THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF
THE PROGRAM IS WITH YOU. SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE
COST OF ALL NECESSARY SERVICING, REPAIR OR CORRECTION.
July 2005
12. IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING WILL ANY
COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MAY MODIFY AND/OR REDISTRIBUTE THE
PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR
INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF DATA OR DATA
BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS), EVEN IF SUCH HOLDER OR
OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES.
19-4
END OF TERMS AND CONDITIONS

How to Apply These Terms to Your New Programs
If you develop a new program, and you want it to be of the greatest possible use to the public, the best way to
achieve this is to make it free software which everyone can redistribute and change under these terms.
To do so, attach the following notices to the program. It is safest to attach them to the start of each source file to
most effectively convey the exclusion of warranty; and each file should have at least the "copyright" line and a pointer
to where the full notice is found.
<one line to give the program's name and a brief idea of what it does.>
Copyright (C) 19yy <name of author>
This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; either version 2 of the License, or (at your option) any
later version.
This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even
the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
General Public License for more details.
You should have received a copy of the GNU General Public License along with this program; if not, write to
the Free Software Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
Also add information on how to contact you by electronic and paper mail.
If the program is interactive, make it output a short notice like this when it starts in an interactive mode:
Gnomovision version 69, Copyright (C) 19yy name of author
Gnomovision comes with ABSOLUTELY NO WARRANTY; for details type `show w'.
This is free software, and you are welcome to redistribute it under certain conditions; type `show c' for details.
The hypothetical commands show w and show c should show the appropriate parts of the General Public
License. Of course, the commands you use may be called something other than show w and show c; they could
even be mouse-clicks or menu items--whatever suits your program.
You should also get your employer (if you work as a programmer) or your school, if any, to sign a "copyright disclaimer" for the program, if necessary. Here is a sample; alter the names:
Yoyodyne, Inc., hereby disclaims all copyright interest in the program Gnomovision (which makes passes at
compilers) written by James Hacker.
<signature of Ty Coon>, 1 April 1989
Ty Coon, President of Vice
July 2005
This General Public License does not permit incorporating your program into proprietary programs. If your program is a subroutine library, you may consider it more useful to permit linking proprietary applications with the
library. If this is what you want to do, use the GNU Library General Public License instead of this License.
19-5
July 2005
19-6
Index
20. Index
A
Abbreviations .................................................... 1-4
Abbreviations and unit used throughout this manual .. 1-4
Action message screen ........................................ 6-3
Additional special equipment ....................... 4-1, 4-3
Adjusting the contrast of the LCD screen .............. 5-12
Alphabetical error massage index ........................ 14-4
Ambient temperature .................................. 4-1, 4-2
Analysis ........................................................... 3-5
Analysis data ..................................................... 8-4
Analysis in whole blood (WB) mode ...................... 7-5
Analysis mode ................................................... 8-4
Analysis parameters ............................................ 1-5
Analyzing samples ............................................. 7-9
Appendix ........................................................ 18-1
As-needed maintenance ..................................... 13-1
ASTM communication specifications ................. 15-33
Automatic calibration ........................................ 11-2
Avoidance of biohazards ...................................... 2-2
Avoidance of Infections ....................................... 2-2
Clean aperture of TD (Transducer) chamber

(Drain TD Chamber) .................................. 13-14
Clean TD (transducer) chambers and
diluted sample lines (Shutdown) ..................... 13-3
Clean the sample tube adapter ........................... 13-12
Clean transducer .............................................. 13-4
Clean waste chamber ........................................ 13-7
Cleaning and Maintenance ................................. 13-1
Communication specifications .......................... 15-34
Composition ............................................. 4-1, 4-2
Connecting the bar code reader ........................... 15-6
Connection of power cable ................................... 5-8
Connection procedure ....................................... 15-7
Consumables ................................................. 13-29
Contact address .................................................. 1-2
Control blood .................................................... 2-5
Control material ............................................... 10-1
Control method selection ................................... 10-3
Control methods ............................................... 10-2
D
B
Background check ..................................... 3-5, 7-3
Bar code reader (optional) .................................... 5-7
Bar code reader settings ..................................... 15-8
Bar code safety .................................................. 2-2
Basic instrument settings ..................................... 5-8
Basic operation area ............................................ 6-3
Before installation .............................................. 5-1
Revised August 2006
Calculating the calibration value .......................... 11-6

Calculation of RBC constant ............................. 15-57
Calibrate LCD ............................................... 13-21
Calibration ...................................................... 11-1
CELLCLEAN..................................................... 4-3
Checking instrument status ................................. 13-2
Checking the program ..................................... 15-54
Checking the program version ........................... 15-54
Checks prior to operation ..................................... 7-2
Daily .............................................................. 13-1

Data processing area ........................................... 6-3
Date and time of analysis ..................................... 8-4
Date and time settings ......................................... 5-8
Date/Time ....................................................... 12-4
DC detection method ...................................... 15-55
Delivery, storage until installation ......................... 5-1
Description of the analysis results screen ................ 8-4
Design and function ............................................ 3-1
Detection principle ......................................... 15-55
Device activation/deactivation .............................. 9-1
Dialog screen ..................................................... 6-4
Discriminator .................................................. 17-1
Display ............................................................. 3-6
Display and output of analysis results ..................... 8-1
Display column .................................................. 6-4
Display of analysis results .................................. 7-13
Disposal of materials ........................................... 2-5
Dispose waste fluid ......................................... 13-10
Drain reagent ................................................. 13-19
20-1
Index
E
EIGHTCHECK-3WP ......................................... 4-4

Electric diagram ............................................ 15-59
Electric system .............................................. 15-58
Electromagnetic compatibility (EMC) .................... 2-2
End of operation (Shutdown) ............................. 7-14
Entering a sample ID .......................................... 7-7
Entering from handheld bar code reader (option) .... 10-8
Entering from numerical keys dialog ................... 10-6
Entering target values ....................................... 11-3
Error massage index by function ......................... 14-5
Error messages ................................................ 14-3
Error messages, possible causes and action
to resolve the error ...................................... 14-7
Establishing reference values ............................. 11-2
Every 2 weeks (or every 150 samples) ................. 13-1
Every 3 months (or every 1500 samples) .............. 13-1
Executing the stored data processing program ......... 8-7
Handling of reagent ............................................ 2-3

Hardware specifications .................................... 15-8
Histogram ...................................................... 17-1
Histogram error flags ........................................ 8-12
Histogram parameter ........................................ 17-1
Host settings ................................................... 12-7
Hydrodynamic Focusing DC detection method .... 15-56
F
Fifth analysis screen ........................................... 8-3
Finishing the stored data processing program .......... 8-8
First analysis screen ........................................... 8-1
Flowchart of analysis procedure ........................... 7-1
Format ......................................................... 15-17
Fourth analysis screen ......................................... 8-3
Front view (with the front lower panel removed) ...... 3-1
Functional description ............................ 3-5, 15-56
I
ID ................................................................... 8-4
ID Bar code Specifications ................................ 15-9
Initial operation ................................................. 5-1
Input from handheld bar code reader (option) .......... 7-8
Input via numerical keys dialog ............................ 7-8
Installation ....................................................... 2-2
Installing the program card .............................. 15-54
Instrument setup .............................................. 12-1
Instruments can be used .............................. 4-1, 4-3
Intended purpose ....................................... 4-1, 4-2
Interface protocol ........................................... 15-13
Introduction .............................................. 1-1, 7-1
L
LAN communication ...................................... 15-16
Latest sample .................................................... 8-1
Levey-Jennings control ......................... 10-2, 10-15
List of consumables ....................................... 13-29
List of provided parts for pocH-100iV Diff ............. 5-1
G
M
Main screen/Menu screen .................................... 6-1
Maintenance ..................................................... 2-5
Maintenance Record pocH-100iV Diff ................. 18-1
Maintenance schedule ....................................... 13-1
Manual analysis.................................................. 8-8
Manual analysis flag ........................................... 8-6
Manual calibration ........................................... 11-6
Measuring unit hydraulic system block diagram ... 15-57
Menu tree ......................................................... 6-7
Methodology ............................................ 4-1, 4-2
Revised August 2006
General faults, instrument failure ........................ 14-2

General Information ........................................... 2-1
General information ......................................... 12-1
Glossary ........................................................ 17-1
GNU GENERAL PUBLIC LICENSE ................. 19-1
20-2
Index
N
Names .............................................................. 1-4
Network settings .............................................. 12-8
Non-Cyanide Hemoglobin analysis method ......... 15-56
Numerical keys dialog ......................................... 6-5
O
Operation .......................................................... 6-1
Optional parts list ............................................. 18-4
Ordering of supplies and replacement parts ............. 1-2
Output ........................................... 3-6, 8-12, 9-1
Output format for host computer ....................... 15-13
Output on built-in thermal printer ........................ 15-5
Overview .......................................................... 3-1
Q
QC settings ..................................................... 12-7
Quality Control .................................................. 7-4
Quality control ................................................. 10-1
Quality control process flow ............................... 10-5
R
RBC histogram .................................................. 8-6
Reagents ........................................................... 4-1
Rear side .......................................................... 3-4
Recording QC data ......................................... 10-20
Relocation of discrimination position ..................... 8-9
Removal of fixing tape ........................................ 5-4
Remove a clog from transducer aperture ............. 13-13
Removing the program card ............................. 15-53
Replace reagent .............................................. 13-24
Replace thermal printer paper ........................... 13-28
Re-selection of species ........................................ 8-8
Resistance measuring method ............................. 17-1
Right view (with the right side panel opened) ........... 3-2
Revised August 2006
Paper feed ......................................................... 9-2

Parameter calculation .......................................... 3-6
Password setting .............................................. 12-9
Password setting procedure ................................ 12-9
Perform auto rinse ............................................ 13-9
Performance characteristics/Specifications ............ 15-1
Performing a quality control ............................. 10-13
Performing analyses .......................................... 11-4
Peripheral devices .............................................. 5-7
Personnel .......................................................... 2-8
Photometric measuring method ........................... 17-1
Physical layer (hardware) ................................. 15-34
PLT histogram ................................................... 8-6
pocH-pack D ..................................................... 4-1
pocH-pack LVD ................................................. 4-2
Possible settings ............................................... 12-3
Power cable ....................................................... 7-2
Preparation of installation location ......................... 5-4
Preparation of printer paper .................................. 5-6
Preparations .................................................... 10-6
Preparations for the next analysis ........................... 3-6
Presentation layer
(pocH-100iV Diff communication) ............... 15-40
Print and output of analysis results ....................... 7-13

Print error log ................................................ 14-15
Print formats ................................................... 15-5
Print set values ............................................... 12-12
Printer paper ...................................................... 7-2
Printer settings ................................................. 12-8
Printing calibration history ................................. 11-9
Procedure ....................................................... 10-1
Program card replacement procedure .................. 15-53
Putting the sticker ............................................... 5-5
20-3
Index
S
Safety information ............................................. 2-1

Safety information in this manual .......................... 1-3
Sample Analysis ................................................ 7-1
Sample analysis ................................................. 7-1
Sample aspiration .............................................. 3-5
Sample collection tube settings ........................... 5-10
Sample preparation ............................................ 7-5
Samples used for calibration .............................. 11-1
Screen display ................................................... 6-1
Second analysis screen ........................................ 8-2
Select mode and parameter ................................ 11-2
Selections ......................................................... 6-4
Self-check ........................................................ 7-3
Separating the adapter ..................................... 15-54
Serial communications .................................... 15-13
Service and maintenance ..................................... 1-2
Setting Sequence ........................................... 13-22
Settings for control blood information .................. 10-5
Signal tones ...................................................... 6-8
Software ........................................................ 15-8
Specified Conditions of Use ................................. 2-1
Specimen ................................................. 4-1, 4-2
Specimen requirements ....................................... 7-4
Starting ............................................................ 3-5
Storage and shelf life after first opening ................. 4-1
Stored data ....................................................... 8-7
Switching ON ................................................... 7-3
Symbols used on the label ................................... 4-5
System area ...................................................... 6-1
System fuse replacement ................................. 13-27
System limitations - interferences ....................... 15-4
System of units .............................................. 15-51
System setup ................................................... 12-3
Unit ................................................................ 1-4

Updating calibration values ..................... 11-5, 11-6
User information settings .................................. 12-7
W
Warning labels on the instrument .......................... 2-7
Warranty ........................................................ 16-1
WBC histogram ................................................. 8-6
Weekly .......................................................... 18-1
X
X control
............................................ 10-2, 10-13
Revised August 2006
Technical Information ...................................... 15-1

Third analysis screen .......................................... 8-2
Timer functions ................................................. 6-8
Troubleshooting .............................................. 14-1
20-4

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For Veterinary Use

AUTOMATED HEMATOLOGY ANALYZER

CHAPTER2: Safety Information

Display and Output of Analysis Results

CHAPTER10: Quality Control

Code No. 461-2406-6

Revised March 2006

Introduction ......................................................... 1-1

Safety information in this manual ........................................... 1-3

Safety Information ............................................... 2-1

Specified conditions of use ..................................................... 2-1

Design and Function ........................................... 3-1

Overview ................................................................................. 3-1

Reagents .............................................................. 4-1

pocH-pack D ........................................................................... 4-1

Initial Operation ................................................... 5-1

Delivery, storage until installation ........................................... 5-1

Sysmex pocH-100iV Diff Instructions for Use

Screen display ........................................................................ 6-1

Sample Analysis ..................................................7-1

Introduction ............................................................................. 7-1

Display and Output of Analysis Results ............8-1

Latest sample ......................................................................... 8-1

Quality Control ...................................................10-1

Control material .................................................................... 10-1

Samples used for calibration ................................................ 11-1

Sysmex pocH-100iV Diff Instructions for Use

Instrument Setup ............................................... 12-1

General information .............................................................. 12-1

Cleaning and Maintenance ............................... 13-1

Maintenance schedule .......................................................... 13-1

Troubleshooting ................................................ 14-1

General faults, instrument failure .......................................... 14-2

Revised August 2006

Sysmex pocH-100iV Diff Instructions for Use

Technical Information ...................................... 15-1

GNU GENERAL PUBLIC LICENSE ...................19-1

Revised March 2006

Sysmex pocH-100iV Diff Instructions for Use

Revised March 2006

Sysmex pocH-100iV Diff Instructions for Use

SYSMEX EUROPE GmbH

SYSMEX AMERICA, Inc.

SYSMEX ASIA PACIFIC PTE

Ordering of supplies and replacement parts

Revised March 2006

Please contact the Service Department of your local Sysmex

Sysmex pocH-100iV Diff Instructions for Use

1.1 Safety information in this manual

Caution, risk of danger, electric shock

Revised March 2006

Sysmex pocH-100iV Diff Instructions for Use

Sysmex, pocH-100iV Diff, pocH-pack and EIGHTCHECK are

CELLCLEAN is a registered trademark of SYSMEX

CAPIJECT is a registered trademark of Terumo Corporation.

ETHERNET is a registered trademark of Xerox Corporation.

MICROTAINER is a registered trademark of Becton Dickinson

SARSTEDT, S-MONOVETTE and MICROVETTE are registered

ISBT128 (International Society of Blood Transfusion) is copyrighted

Linux is a registered trademark of Linus Torvalds.

1.3 Abbreviations and units used throughout this manual

Complete Blood cell Count

deciliter (0.1 liter)

femtoliter (10 -15 liter)