Summary:

Essentialknowledge4.B.1:Interactionsbetweenmoleculesaffecttheirstructureandfunction.

a.Changeinthestructureofamolecularsystemmayresultinachangeofthefunctionofthe
system.

b.Theshapeofenzymes,activesitesandinteractionwithspecificmoleculesareessentialfor
basicfunctioningoftheenzyme.
1.Foranenzymemediatedchemicalreactiontooccur,thesubstratemustbe
complementarytothesurfaceproperties(shapeandcharge)oftheactivesite.Inother
words,thesubstratemustfitintotheenzymesactivesite.
2.Cofactorsandcoenzymesaffectenzymefunctionthisinteractionrelatestoastructural
changethatalterstheactivityrateoftheenzyme.Theenzymemayonlybecomeactive
whenalltheappropriatecofactorsorcoenzymesarepresentandbindtotheappropriate
sitesontheenzyme.

c.Othermoleculesandtheenvironmentinwhichtheenzymeactscanenhanceorinhibitenzyme
activity.Moleculescanbindreversiblyorirreversiblytotheactiveorallostericsites,changing
theactivityoftheenzyme.

d.Thechangeinfunctionofanenzymecanbeinterpretedfromdataregardingthe
concentrationsofproductorsubstrateasafunctionoftime.Theserepresentationsdemonstrate
therelationshipbetweenanenzymesactivity,thedisappearanceofsubstrate,and/orpresenceof
acompetitiveinhibitor.

Artifact:


Standardslearned:4.B.1.b124.B.1.c.

Reflection:
IchosetomakeaquizletforthisartifactbecauseIhadtroubleretainingalloftheinformation
aboutenzymes,andIstillstrugglewiththis.Butbydoingthis,Ihadtogobacktomynotesand
decideformyselfwhattheimportantkeypointswere.OnethingthatIwasremindedabout
throughthisactivitywasthefactorsthataffectenzymesandhowtheyaffectthemdifferentlyas
well.Mostimportantly,Ireviewedthebasics,likewhatasubstrateandactivesitewere.A
substrateisthereactantthatanenzymeactsonandtheactivesiteisthespecificregionofthe
enzymemoleculethatactuallybindstothesubstrate.Ialsowentbackovertheirreversibleand

reversibleinhibitorsandhowreversibleinhibitionusesweakinteractionsandirreversibleuses
covalentbonds.