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Choosing The Right

Creatine Supplement

Dai H. Le

Many athletes, bodybuilders, and others used synthetic creatine supplement to
gain muscle, mass, and strength. Not many may know, but 95% of the human bodys
creatine is located in the skeletal muscle and cannot produce enough creatine from the
food we obtained daily, such as meats and fish. It is a reason to why many companies
took advantage this million-dollar idea and manufactured synthetic creatine to give the
gym goers the supplement they need in their trainings. Currently, there are a lot of
creatine supplements in the market, almost too many for one is able to choose. Ergo, the
purpose of this project is to identify between a selections of creatine products to give the
consumer, like myself, the true facts of what he or she is actually consumed.
1) Determining the Melting Point:

The purpose of determining the melting point is to find out the purity of the
creatine supplement. The initial melting point of pure creatine is 255 Celsius.

a) Placed ample amount of creatine (Cr) powder on to watch glass.

b) Using the capillary tube to assimilate the creatine powder.
c) Place the thermometer and capillary tube with the assimilated creatine powder to
observe the melting point.
2) Thin-Layer Chromatography:

The key idea of Thin-Layer Chromatography (TLC) is to find the interaction of

each creatine products. By observing the mobility of each creatine supplements,
we can determine their solubility based on

a) Draw a straight line across the TLC (along the silica gel).
b) Take a spotter and dip it into the reaction flask, then put a spot in the middle of
the line.
c) Pour a small amount of solvent (water) into the developing chamber.
d) Submerge the TLC plate into the chamber and close the lid.
e) Observer for mobility.
3) Gas Chromatography Mass Spectrometry:

The method of GC-Mass Spectrometry is to separate the gas phase and stationary
phase. The idea of this process is to measure the quantitative and the qualitative
amount of the separated components in the creatine supplements.

a) Placed each creatine supplements in each individual tubes.

b) Extract the creatine supplements in a solvent (50 mL of ethanol)
c) Carefully extract the solute under fume hood.

d) Wait an ample time for the solute to dissolve in the solvent.

e) Test the solution with Gas chromatograph.
Melting Point:

Capillary (melting point) tubes

Watch glass
Melting point apparatus
Creatine Supplements

Gas Chromatography:

Gas Chromatograph
Solvent (Ethanol)
Creatine Supplements

TLC Chromatography:

Water solvent
Developing Chamber
Creatine Supplements