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Dela Rosa, M.L., Diaz, K.I.M, Domingo, M.A., Dungca, M.K., Gabayan, M.
2H Pharmacy, Faculty of Pharmacy
University of Santo Tomas
Abstract
The experiment was done to determine the electrometric pH of the buffers
and samples with the use of the pH meter and to differentiate them by
colorimetric determination method through using different acid-base
indicators. The indicators used were thymol blue, which is a diprotic acid,
bromophenol blue, bromocresol green, bromocresol purple, phenol red,
methyl red, methyl orange, and phenolphthalein. The buffer that was
assigned to be made was an acetate buffer with a pH of 5 which was
checked by the pH meter. The samples used were the coconut juice and
distilled water. Distilled water had a pH of 5 given that one of the columns in
the pH meter matched into that of the distilled water. On the other hand, the
coconut juice which had a pH of 5.3 was determined by the pH meter by
using the electrometric determination method.
Introduction
All biological processes are
greatly affected by pH (Crisostomo
A.C.,et al.2010). The term pH is
used to identify the concentration
of [H+], thus the pH of a solution is
simply the negative logarithm of
[H+].
The pH of a solution is
greatly
dependent
on
the
concentration of [H+] ions, thus
buffers are required to maintain the
pH by binding the [H+] ions which
in turn stabilizes the changes in pH.
(Concepts in Biochemistry,n.d.)
A buffer solution is a mixture of a
weak acid and its conjugate base,
or a weak base and a conjugate
Henderson-Hasselbach
equation
was used given that it shows the
Procedure
Preparation of reagents and buffer:
500 mL of 6.0 M of HCl and
6.0 M of NaOH was prepared and
properly
labeled.
Acetic
acid
(CH3COOH) and sodium acetate
(Na2CH3COO)
were
used
in
preparing the 250 mL buffer. 6
grams of acetic acid was mixed
with 0.01 L of NaOH and was added
with water to fill up the 250mL
pK = 4.80 pH = 5.00
pH = pKa + log [X]/[Y]
5.00 = 4.70 + log (x)
X = 0.50
1.50 = 0.10
1
Y
Y = 0.06L
Y = 10-0.06
Y = 0.04
pH = pKa + log [salt]/[acid]
5.00 = 4.70 + log X
0.3 = log X
X = 0.50
container
was
Acetate
Electrometric Determination of pH
Calorimetric Determination of pH
A. Preparation
of
color
standards using the buffer
solutions:
Six test tubes was prepared and
labeled with the pH of the buffer
and acid-base indicator to be
added. 5 mL of the buffer was
placed in each test tube and 2
drops of an acid-base indicator was
added. The mixture was shaken
and the resulting color was noted.
The procedure was repeated using
pH
7.0
yellow
7.5
yellow
Distill
ed
Water
Sampl
e
Bromophenol
Blue
Bromocresol
Green
Bromocresol
Purple
Phenol Red
Methyl Red
yellow
yellow
blue
blue
blue
8.0
yello
w
blue
yellow
yellow
blue
blue
blue
blue
blue
blue
blue
yellow
yellow
yellow
purple
purple
yellow
yellow
pink
yellow
orange
orange
yellow
orange
yellow
yellow
orange
yellow
orange
Methyl
Orange
Phenolphthal
ein
yellow
yellow
orange
orange
orange
orange
orange
colorle
ss
colorle
ss
colorle
ss
colorle
ss
colorle
ss
purpl
e
red
yello
w
oran
ge
pink
yellow
yellow
pink
purpl
e
red
yello
w
oran
ge
pink
colorle
ss
colorle
ss
12.0
blue
yellow
yellow
blue
blue
blue
Conclusion
Determination of the pH of a
certain substance or solution
through acid-base color indicators
shows the different range of
varying colors when a color
indicator is added to a solution with
a certain pH. Chemical reactions
Concepts in Biochemistry,n.d,
Retrieved
from:
http://www.wiley.com/college/boyer
/0470003790/reviews/pH/ph_ioniza
tion_ph.htm
References:
Crisostomo
A.C.,et
al.(2010).
Laboratory Manual in General
Biochemistry,
Quezon
City,
Philippines.
Physical
Properties:
Water
Chemistry: pH, from Discovery of
Estuarine
Environments
(DOEE),n.d., para. 1 Retrieved
from:
http://omp.gso.uri.edu/ompweb/do
ee/science/physical/chph2.htm